Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F80 I0 R1	1148	65.4	3296958	93.8%	3092546	131.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,551,327	A→C	11.3%	intergenic (+44/+82)	groL → / ← pykA	chaperonin GroEL/pyruvate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,551,327	0	A	C	11.3%	200.8 / 20.3	80	intergenic (+44/+82)	groL/pykA	chaperonin GroEL/pyruvate kinase II
Reads supporting (aligned to +/- strand): ref base A (39/32); new base C (5/4); total (44/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.82e-01

TGATGATCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGATGGTGTGG  >  NC_002947/1551180‑1551472

tgatgaTCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCgaga                                                                                                                                                 >  2:581233/1‑150 (MQ=255)

  atgatCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCTCGCGAAc                                                                                                                                               >  1:1008737/1‑150 (MQ=255)

    gatCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCg                                                                                                                                             <  2:824435/150‑1 (MQ=255)

    gatCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCg                                                                                                                                             >  2:1633551/1‑150 (MQ=255)

     atCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCTCGCGAACCgg                                                                                                                                            <  2:1008737/150‑1 (MQ=255)

       caccacCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCTCGCGAACCgggg                                                                                                                                          <  1:70944/150‑1 (MQ=255)

         ccaccGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCt                                                                                                                                        >  1:712594/1‑150 (MQ=255)

          caccGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCtt                                                                                                                                       >  1:1114862/1‑150 (MQ=255)

                    aTGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCt                                                                                                                             >  2:1512713/1‑150 (MQ=255)

                          gCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCggg                                                                                                                       <  2:712594/150‑1 (MQ=255)

                                 tGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAACAAGCGCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCtt                                                                                                                >  1:766381/1‑150 (MQ=255)

                                    cGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCgc                                                                                                             <  1:1494378/150‑1 (MQ=255)

                                          aCAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCgcagcctt                                                                                                       >  1:1454412/1‑147 (MQ=255)

                                            aaGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAg                                                                                                     >  1:614557/1‑150 (MQ=255)

                                            aaGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCATCTTCGCAGCACAAg                                                                                                     >  2:1140099/1‑150 (MQ=255)

                                              gCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACTAGGc                                                                                                   >  2:538564/1‑150 (MQ=255)

                                              gCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGc                                                                                                   >  2:570368/1‑150 (MQ=255)

                                               ccAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCt                                                                                                  >  2:1632522/1‑150 (MQ=255)

                                                        ggCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAg                                                                                         <  2:284313/150‑1 (MQ=255)

                                                         gCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGc                                                                                        >  1:1272669/1‑150 (MQ=255)

                                                            tGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCTGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCtt                                                                                     <  1:1633551/150‑1 (MQ=255)

                                                            tGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCtt                                                                                     >  2:1512350/1‑150 (MQ=255)

                                                            tGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCtt                                                                                     >  2:66475/1‑150 (MQ=255)

                                                             gCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTtg                                                                                    <  2:614557/150‑1 (MQ=255)

                                                               cTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTtgtg                                                                                  >  1:1528666/1‑150 (MQ=255)

                                                                tGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACTAGGCTGCGCTTGAGCCTTGTGc                                                                                 <  1:538564/150‑1 (MQ=255)

                                                                       ggtggcatgggtggcatgGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGaaa                                                                          <  2:1114862/150‑1 (MQ=255)

                                                                           gcatgggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATAAAAAAGCCCCGGTTCTCGCGAACCGGGGCtttttgat                                                                                                                                 >  1:631968/1‑88 (MQ=25)

                                                                           gcatgggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATAAAAAAGCCCCGGTTCTCGCGAACCGGGGCTTTTTGAt                                                                                                                                 <  2:631968/91‑1 (MQ=25)

                                                                            catgggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGt                                                                     <  2:1272669/150‑1 (MQ=255)

                                                                              tgggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCgg                                                                                                                                            >  1:971088/1‑77 (MQ=255)

                                                                              tgggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCgg                                                                                                                                            <  2:971088/77‑1 (MQ=255)

                                                                                ggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCgg                                                                                                                        <  2:629311/95‑1 (MQ=255)

                                                                                ggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCgg                                                                                                                        >  1:629311/1‑95 (MQ=255)

                                                                                   ggcatgtgCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGCACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCaa                                                              <  1:1451985/150‑1 (MQ=255)

                                                                                      atgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCg                                                           >  2:89415/1‑150 (MQ=255)

                                                                                         ggcggcATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGAcc                                                        <  2:884322/150‑1 (MQ=255)

                                                                                             gcATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCggg                                                                                                                       <  1:510864/83‑1 (MQ=255)

                                                                                             gcATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCggg                                                                                                                       >  2:510864/1‑83 (MQ=255)

                                                                                             gcATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCg                                                    >  2:1445295/1‑150 (MQ=255)

                                                                                              cATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGt                                                                                                                           <  1:702763/78‑1 (MQ=255)

                                                                                              cATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGt                                                                                                                           >  2:702763/1‑78 (MQ=255)

                                                                                               atgatgTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGa                                                  <  1:581233/150‑1 (MQ=255)

                                                                                               atgatgTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGa                                                  <  1:1512350/150‑1 (MQ=255)

                                                                                                  atgTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGa                                                                                          >  2:185733/1‑107 (MQ=255)

                                                                                                  atgTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGa                                                                                          >  2:185479/1‑107 (MQ=255)

                                                                                                  atgTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGa                                                                                          <  1:185733/107‑1 (MQ=255)

                                                                                                  atgTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGa                                                                                          <  1:185479/107‑1 (MQ=255)

                                                                                                     tAAGCCCGAATTACCCACCTGCACCAGCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCg                                            >  2:1335405/1‑150 (MQ=255)

                                                                                                     tAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCa                                                       >  2:648574/1‑139 (MQ=255)

                                                                                                     tAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCa                                                       <  1:648574/139‑1 (MQ=255)

                                                                                                         ccagccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGt                                        >  2:486422/1‑150 (MQ=255)

                                                                                                         ccagccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGt                                        >  2:926795/1‑150 (MQ=255)

                                                                                                             ccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAg                                    <  1:66475/150‑1 (MQ=255)

                                                                                                                  ccccccTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCt                               <  1:570368/150‑1 (MQ=255)

                                                                                                                   cccccTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGa                                                                       >  1:401628/1‑109 (MQ=255)

                                                                                                                   cccccTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCtt                              >  2:459959/1‑150 (MQ=255)

                                                                                                                   cccccTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCtt                              >  2:847667/1‑150 (MQ=255)

                                                                                                                   cccccTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTCTCGTGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGa                                                                       <  2:401628/109‑1 (MQ=255)

                                                                                                                    ccccTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAAc                                                             <  2:600702/118‑1 (MQ=255)

                                                                                                                    ccccTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAAc                                                             >  1:600702/1‑118 (MQ=255)

                                                                                                                             cATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCGTTTTTATTGCTGTCGGGGCCTCTCCGCAGCACACGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGGGGATGAACCGACAAGCGGATCACAAACGTGAAGGATATTAATGcccgtg                    >  2:1569865/1‑146 (MQ=255)

                                                                                                                             aataaaaaaGCCCCGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTg                    >  2:1606851/5‑150 (MQ=255)

                                                                                                                              aTCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGt                                        <  1:613691/129‑1 (MQ=255)

                                                                                                                              aTCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGt                                        >  2:613691/1‑129 (MQ=255)

                                                                                                                               tCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGt                  <  1:926795/150‑1 (MQ=255)

                                                                                                                                 aaaaaGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGc                                                                                        >  1:312862/1‑78 (MQ=255)

                                                                                                                                 aaaaaGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGc                                                                                        <  2:312862/78‑1 (MQ=255)

                                                                                                                                  aaaaGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCAt                           >  2:1557165/1‑138 (MQ=255)

                                                                                                                                  aaaaGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCAt                           <  1:1557165/138‑1 (MQ=255)

                                                                                                                                  aaaaGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGcc               >  2:267064/1‑150 (MQ=255)

                                                                                                                                   aaaGCCCCGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAg                                                                      >  1:1398489/1‑94 (MQ=255)

                                                                                                                                   aaaGCCCCGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAg                                                                      <  2:1398489/94‑1 (MQ=255)

                                                                                                                                     aGCCCCGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCAcc            >  1:858505/1‑150 (MQ=255)

                                                                                                                                     aGCCCCGGTTCGCGAGAACCGTGGCTTTTTTATTGCTGTCGGGGc                                                                                                                     <  2:1006225/45‑1 (MQ=255)

                                                                                                                                     aGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGc                                                                                                                     >  1:1006225/1‑45 (MQ=255)

                                                                                                                                      gCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGATGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGAGCTTCATGCCATGGGTGCCACCg           >  1:690330/1‑150 (MQ=255)

                                                                                                                                       ccccGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGa          <  2:1528666/150‑1 (MQ=255)

                                                                                                                                         ccGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTtgtg                                                                                  >  2:1314438/1‑76 (MQ=255)

                                                                                                                                         ccGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTtgtg                                                                                  <  1:1314438/76‑1 (MQ=255)

                                                                                                                                               cgcgAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGATGGTGTgg  <  1:267064/150‑1 (MQ=255)

TGATGATCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGATGGTGTGG  >  NC_002947/1551180‑1551472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A