Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F91 I0 R1
|
918 |
53.4 |
2573728 |
94.2% |
2424451 |
136.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947 |
1,551,327 |
A→C |
17.0% |
intergenic (+44/+82) |
groL → / ← pykA |
chaperonin GroEL/pyruvate kinase II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947 | 1,551,327 | 0 | A | C | 17.0%
| 106.6
/ 22.5
| 59 | intergenic (+44/+82) | groL/pykA | chaperonin GroEL/pyruvate kinase II |
| Reads supporting (aligned to +/- strand): ref base A (26/23); new base C (5/5); total (31/28) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01 |
ATCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGATGGT > NC_002947/1551185‑1551468
|
atCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCgg < 2:290904/150‑1 (MQ=255)
tCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCggg < 2:1215939/150‑1 (MQ=255)
cagcgccGAAGCCATGGTTTGCGACCTGCCGGAAGCCGAGCCAGCTGCTGGCATGCCTGGTATTGGTGGCATGGGTGGCCTGCCCGGCATGATGTAAGCCAGCCTTGCACTCCTGCATCATCAAAAAGCCCCGGTTCTCGCGCACCgggg < 2:1241830/145‑1 (MQ=255)
caccacCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCgggg < 1:416957/150‑1 (MQ=255)
caccacCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATAAAAAAGCCCCGGTTCTCGCGAACCgggg < 2:567322/150‑1 (MQ=255)
aaGCCATGGTTGCCGGCCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTa < 2:306339/150‑1 (MQ=255)
gCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTAtt < 2:691934/150‑1 (MQ=255)
ggTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCga > 1:1105747/1‑126 (MQ=255)
ggTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCga < 2:1105747/126‑1 (MQ=255)
ccGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCgggg < 2:608114/150‑1 (MQ=255)
ggAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCgca > 1:182667/1‑150 (MQ=255)
aGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCGACAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTgc < 1:487421/150‑1 (MQ=255)
tgctgGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCt < 2:182667/150‑1 (MQ=255)
ctgGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTg > 1:712186/1‑150 (MQ=255)
ccTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTtgt < 1:309387/150‑1 (MQ=255)
ccTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTtgt > 1:165228/1‑150 (MQ=255)
atatGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCTCGCGAACCGGGGc > 2:1181291/1‑92 (MQ=255)
atatGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCTCGCGAACCGGGGc < 1:1181291/92‑1 (MQ=255)
tatGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCtgt < 2:712186/150‑1 (MQ=255)
gggtggcatgggtggcatGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGaa > 1:558192/1‑150 (MQ=255)
gcatgggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAg > 2:42954/1‑150 (MQ=255)
tgggtggcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTgg > 1:552909/1‑150 (MQ=255)
atgggAGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCACCCGGTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTTAGCCTTGTGCTGGGAAATGAGTGGATCAACCg > 2:500547/1‑150 (MQ=255)
tgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGa > 1:3116/1‑150 (MQ=255)
gatgTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATc > 1:78771/1‑150 (MQ=255)
gTAAGCCAGCCTTCCCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCAcc < 2:442005/150‑1 (MQ=255)
tAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGTCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAAATAGTGGTGCAACCGACCAGCGGATCACCg > 1:914053/1‑150 (MQ=255)
tAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAACCTTTTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCg > 1:914143/1‑150 (MQ=255)
aaGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGAt > 1:464384/1‑144 (MQ=255)
aaGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGAt > 1:463874/1‑144 (MQ=255)
aaGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGAt < 2:464384/144‑1 (MQ=255)
aaGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGAt < 2:463874/144‑1 (MQ=255)
atccagccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGAc < 1:500547/148‑1 (MQ=255)
ccagccTTACCCCCCTGCACCATAAAGACATGCTGGCCAGCGATGACCGTGGCGTCTTTATTGCTGTCGGGGCCTTTCCGAATTCCCGGTCGGCGCCGGAGCCTTGTGCTGTGAAACGAGTGGATCAACCCGCCAACGGAGCACCGACGt > 1:1254847/1‑150 (MQ=255)
ttACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGa > 1:821904/1‑150 (MQ=255)
tACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTgcgc > 2:953183/1‑89 (MQ=255)
tACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTgcgc < 1:953183/89‑1 (MQ=255)
aCCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGc < 2:864906/65‑1 (MQ=255)
aCCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGc > 1:864906/1‑65 (MQ=255)
cccccTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCtt > 2:165237/1‑150 (MQ=255)
ccccTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGc > 2:1277300/1‑42 (MQ=255)
ccccTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGc < 1:1277300/42‑1 (MQ=255)
tGCACCATCAAAAAGCCCCGGTTCGCGAGAACGGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGc < 2:558192/150‑1 (MQ=255)
cATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTg < 2:1254847/150‑1 (MQ=255)
aTCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTgg > 2:548318/1‑150 (MQ=255)
aaaGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATGGCTGTCGGGGCCTCTTCGCAGCACAAGGCGGCGCTCGAGCCTTGGGCAGTGAAACGAGTGGATTAACCGACCATCGGATCACCGACGTGCATGAACTTCATACCATTGGTGCCa > 2:902775/1‑150 (MQ=255)
aaGCCCCGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCAc > 2:862993/1‑150 (MQ=255)
aaGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCAc < 2:78771/150‑1 (MQ=255)
aaGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCAc < 1:42954/150‑1 (MQ=255)
gCCCCGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATGAACCGACCAGCGGATCAGCGACGTGCCGGATCTTCATGCCCTTGGTGCCACCg > 1:58685/1‑150 (MQ=255)
ccccGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGa > 1:1226837/1‑94 (MQ=255)
ccccGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGa < 2:1226837/94‑1 (MQ=255)
ccccGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGc < 1:889013/122‑1 (MQ=255)
ccccGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGc > 2:889013/1‑122 (MQ=255)
ccccGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGa > 1:236327/1‑150 (MQ=255)
cccGGTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGAt > 2:321531/1‑150 (MQ=255)
cccGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCaa < 1:155338/97‑1 (MQ=255)
cccGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCaa > 2:155338/1‑97 (MQ=255)
cccGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGAt > 1:132134/1‑150 (MQ=255)
ccGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGATg > 2:563065/1‑150 (MQ=255)
ggTTCTCGCGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGATGgt < 1:321531/150‑1 (MQ=255)
|
ATCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGATGGT > NC_002947/1551185‑1551468
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
