Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,718,191 A→G T98A (ACT→GCT)  slyB → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,718,1910AG100.0% 402.9 / NA 124T98A (ACT→GCT) slyBputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (51/73);  total (51/73)

Only 100 of 129 total aligned reads displayed.

TGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAACTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTA  >  NC_000913/1718103‑1718280
                                                                                        |                                                                                         
tGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTgg                                                                                       >  1:1113280/1‑93 (MQ=255)
tGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTgg                                                                                       <  1:2769250/93‑1 (MQ=255)
tGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTgg                                                                                       >  1:2401942/1‑93 (MQ=255)
tGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTgg                                                                                       <  2:2164553/93‑1 (MQ=255)
  ttCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTc                                                                                     <  1:1944194/93‑1 (MQ=255)
  ttCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTc                                                                                     <  1:2538488/93‑1 (MQ=255)
     ttGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAgg                                                                                  <  2:1493625/93‑1 (MQ=255)
     ttGGTGGTTTCCTAGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAgg                                                                                  <  2:1142201/93‑1 (MQ=255)
      tggtggTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAggg                                                                                 <  1:3519338/93‑1 (MQ=255)
       ggtggtTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGc                                                                                <  2:2307134/93‑1 (MQ=255)
          ggtTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTa                                                                             >  2:4589783/1‑93 (MQ=255)
           gtTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTAc                                                                            <  1:415367/93‑1 (MQ=255)
              tCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACaga                                                                         >  2:287793/1‑93 (MQ=255)
              tCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACaga                                                                         <  1:4162641/93‑1 (MQ=255)
               ccTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACagag                                                                        <  1:1725802/93‑1 (MQ=255)
                cTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGt                                                                       >  1:2180115/1‑93 (MQ=255)
                cTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGAGGCGTAGCTGGTCAGGGCGTACagagg                                                                       >  1:3001500/1‑92 (MQ=255)
                  ggggAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGc                                                                     <  1:637044/93‑1 (MQ=255)
                   gggAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCa                                                                    <  2:1034034/93‑1 (MQ=255)
                   gggAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCa                                                                    >  2:715321/1‑93 (MQ=255)
                   gggAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCa                                                                    <  1:4672723/93‑1 (MQ=255)
                       aTACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGa                                                                >  1:4300967/1‑93 (MQ=255)
                       aTACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGGGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGa                                                                >  1:504357/1‑93 (MQ=255)
                           tGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAAcga                                                            >  1:2800511/1‑91 (MQ=255)
                            gTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACaaa                                                           >  1:2823051/1‑93 (MQ=255)
                              tggtggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAAc                                                         <  1:1903784/93‑1 (MQ=255)
                               ggtggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACg                                                        >  2:4182197/1‑93 (MQ=255)
                                 tggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCa                                                      <  1:3825205/93‑1 (MQ=255)
                                 tggCGGAACCGGGAGTTCTCTGGCTACTGCAGCAGGAGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGGGCAATGAAGAAAACGCa                                                      >  1:2979424/1‑93 (MQ=255)
                                  ggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCTTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAg                                                     <  1:3238629/93‑1 (MQ=255)
                                  ggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAg                                                     <  2:1557715/93‑1 (MQ=255)
                                  ggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAg                                                     <  1:422091/93‑1 (MQ=255)
                                    gggAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAggg                                                   <  2:182916/92‑1 (MQ=255)
                                    cGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAggg                                                   <  1:1095769/93‑1 (MQ=255)
                                    cGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAggg                                                   <  2:4114853/93‑1 (MQ=255)
                                     ggAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGgt                                                  <  1:3738109/93‑1 (MQ=255)
                                     ggAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGgt                                                  >  2:4186638/1‑93 (MQ=255)
                                     ggAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGgt                                                  <  2:3913136/93‑1 (MQ=255)
                                      gAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGgtg                                                 <  2:2750638/93‑1 (MQ=255)
                                      gAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGgtg                                                 <  2:4662579/93‑1 (MQ=255)
                                      gAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGgtg                                                 <  1:176786/93‑1 (MQ=255)
                                      gAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTCTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGgtg                                                 <  2:3639818/93‑1 (MQ=255)
                                        aCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTc                                               >  2:3003704/1‑93 (MQ=255)
                                         ccGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCg                                              >  1:3923323/1‑93 (MQ=255)
                                         ccGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCg                                              >  2:3073712/1‑93 (MQ=255)
                                          cGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGa                                             <  1:360495/93‑1 (MQ=255)
                                          cGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGa                                             <  2:2304098/93‑1 (MQ=255)
                                           gggCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAg                                            >  2:2410320/1‑93 (MQ=255)
                                            ggCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGc                                           <  2:3560433/93‑1 (MQ=255)
                                             gCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCt                                          >  1:787833/1‑93 (MQ=255)
                                              cGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTg                                         >  2:1703482/1‑93 (MQ=255)
                                              cGTTCTCCGGCTACGGCAGCAGGCGCTGTGGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTg                                         <  2:4711082/93‑1 (MQ=255)
                                               gTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTgg                                        <  1:3207822/93‑1 (MQ=255)
                                                ttCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGa                                       >  2:2575177/1‑93 (MQ=255)
                                                ttCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGa                                       >  2:138471/1‑93 (MQ=255)
                                                ttCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGa                                       >  2:2917847/1‑93 (MQ=255)
                                                 tctcTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGaa                                      <  2:4609667/93‑1 (MQ=255)
                                                 tctcTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGaa                                      <  2:4609668/93‑1 (MQ=255)
                                                 tctcTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGaa                                      >  1:1767466/1‑93 (MQ=255)
                                                  ctctGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGaaa                                     <  1:3960047/93‑1 (MQ=255)
                                                     tGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCGATGAACAAAACGCAGGGTGTCGAGCTGGAAATTc                                  <  2:2403599/93‑1 (MQ=255)
                                                        cTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTa                               >  1:1696591/1‑93 (MQ=255)
                                                        cTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTa                               >  2:2664182/1‑93 (MQ=255)
                                                        cTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTa                               <  1:966520/93‑1 (MQ=255)
                                                         tACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTaa                              >  1:3267785/1‑93 (MQ=255)
                                                          aCTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTaaa                             <  2:1965883/93‑1 (MQ=255)
                                                            tGCAGCAGGCGCTGTTGCAGGTTGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGa                           <  2:3625780/93‑1 (MQ=255)
                                                               agcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGa                        <  2:1718118/93‑1 (MQ=255)
                                                               agcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGa                        <  2:1469457/93‑1 (MQ=255)
                                                                gcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGAt                       <  2:4451555/93‑1 (MQ=255)
                                                                gcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGAt                       <  1:1597851/93‑1 (MQ=255)
                                                                  agGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGTAGGGTGTCGAGCTGGAAATTCGTAAAGACGATgg                     <  2:1265241/93‑1 (MQ=255)
                                                                  agGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATgg                     >  2:2035395/1‑93 (MQ=255)
                                                                  agGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATgg                     >  2:4661637/1‑93 (MQ=255)
                                                                  agGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATgg                     >  1:3421849/1‑93 (MQ=255)
                                                                   ggCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATgga                    >  1:3811578/1‑92 (MQ=255)
                                                                   ggCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATgga                    >  1:3811579/1‑92 (MQ=255)
                                                                   ggCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGt                    >  1:1356088/1‑93 (MQ=255)
                                                                   ggCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGt                    <  1:2711804/93‑1 (MQ=255)
                                                                    gcgcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTa                   <  2:2581148/93‑1 (MQ=255)
                                                                     cgcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTaa                  <  1:2998585/93‑1 (MQ=255)
                                                                     cgcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTaa                  <  2:3331022/93‑1 (MQ=255)
                                                                      gcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAAt                 <  2:4248083/93‑1 (MQ=255)
                                                                       cTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATa                <  1:3473759/93‑1 (MQ=255)
                                                                       cTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATa                >  1:4636613/1‑93 (MQ=255)
                                                                       cTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATa                <  1:1262303/93‑1 (MQ=255)
                                                                       cTGTGGCAGGTGGCGTAGCTGGTGAGGGCGTACAGACTGGAATGAACAAAACGCAGGGTGTCGAGCTGCAAATTCGTAAAGACGATGGTAATa                <  1:4742659/93‑1 (MQ=255)
                                                                        tGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATAc               >  2:3540438/1‑93 (MQ=255)
                                                                          ttGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACca             <  2:1315843/93‑1 (MQ=255)
                                                                          ttGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACca             <  1:461982/93‑1 (MQ=255)
                                                                            gCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAAGGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACcatc           >  1:813990/1‑93 (MQ=255)
                                                                            gCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACcatc           >  1:813840/1‑93 (MQ=255)
                                                                            gCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACcatc           >  2:498867/1‑93 (MQ=255)
                                                                              aGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACcatcat         <  1:3410906/93‑1 (MQ=255)
                                                                               ggTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAtg        >  2:792632/1‑93 (MQ=255)
                                                                                gTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAtgg       <  2:4357429/93‑1 (MQ=255)
                                                                                gTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAtgg       <  2:622398/93‑1 (MQ=255)
                                                                                 tGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGGGTCGAGCTGGAAATTCGTAAAGACGATGGTAACACCATCAtgga      >  2:4125745/1‑92 (MQ=255)
                                                                                  ggCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAtggtg     >  1:4316928/1‑93 (MQ=255)
                                                                                     gTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTa  >  2:3485644/1‑93 (MQ=255)
                                                                                        |                                                                                         
TGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAACTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTA  >  NC_000913/1718103‑1718280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAACTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACA  >  NC_000913/1718098‑1718282
                                                                                             |                                                                                           
GGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGAGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAG                                                                                             >  SRR4302544.3107884/1‑94 (MQ=60)
 GTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGC                                                                                            >  SRR4302544.4731081/1‑94 (MQ=60)
 GTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTAGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGC                                                                                            >  SRR4302544.4461312/1‑94 (MQ=60)
    CTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGG                                                                                         >  SRR4302544.1124384/1‑94 (MQ=60)
    CTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGG                                                                                         >  SRR4302544.2424730/1‑94 (MQ=60)
     TGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGT                                                                                        <  SRR4302544.2795895/94‑1 (MQ=60)
       TTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCA                                                                                      <  SRR4302544.2562866/94‑1 (MQ=60)
       TTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCA                                                                                      <  SRR4302544.1963018/94‑1 (MQ=60)
           TGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGC                                                                                  <  SRR4302544.3552948/94‑1 (MQ=60)
               GGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTAC                                                                              <  SRR4302544.526519/94‑1 (MQ=60)
                GTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACA                                                                             <  SRR4302544.419608/94‑1 (MQ=60)
                   TCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAG                                                                          <  SRR4302544.4203876/94‑1 (MQ=60)
                    CCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGT                                                                         <  SRR4302544.1742735/94‑1 (MQ=60)
                    CCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGT                                                                         >  SRR4302544.2200757/1‑94 (MQ=60)
                    CCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGC                                                                         >  SRR4302544.3444406/1‑94 (MQ=60)
                    CCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGAGGCGTAGCTGGTCAGGGCGTACAGAGG                                                                         >  SRR4302544.3030276/1‑94 (MQ=60)
                       GGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA                                                                      <  SRR4302544.643323/94‑1 (MQ=60)
                        GGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAA                                                                     <  SRR4302544.4718908/94‑1 (MQ=60)
                           AATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGA                                                                  >  SRR4302544.4344846/1‑94 (MQ=60)
                           AATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGGGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGA                                                                  >  SRR4302544.509316/1‑94 (MQ=60)
                            ATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAA                                                                 >  SRR4302544.948218/1‑94 (MQ=60)
                               CTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACGA                                                              >  SRR4302544.2827496/1‑94 (MQ=60)
                                TGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAA                                                             >  SRR4302544.2850331/1‑94 (MQ=60)
                                   TGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACG                                                          <  SRR4302544.1922184/94‑1 (MQ=60)
                                     GTGGCGGAACCGGGAGTTCTCTGGCTACTGCAGCAGGAGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGGGCAATGAAGAAAACGCA                                                        >  SRR4302544.3007998/1‑94 (MQ=60)
                                      TGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAG                                                       <  SRR4302544.3863138/94‑1 (MQ=60)
                                       GGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCTTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGG                                                      <  SRR4302544.3269626/94‑1 (MQ=60)
                                       GGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGG                                                      <  SRR4302544.4702016/94‑1 (MQ=60)
                                       GGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGG                                                      <  SRR4302544.426400/94‑1 (MQ=60)
                                         CGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGT                                                    >  SRR4302544.258015/1‑94 (MQ=60)
                                         CGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGT                                                    <  SRR4302544.1106673/94‑1 (MQ=60)
                                          GGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTG                                                   <  SRR4302544.3775266/94‑1 (MQ=60)
                                           GAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGT                                                  <  SRR4302544.178536/94‑1 (MQ=60)
                                             ACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCG                                                >  SRR4302544.3962295/1‑94 (MQ=60)
                                               CGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAG                                              >  SRR4302544.1750750/1‑94 (MQ=60)
                                               CGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAG                                              <  SRR4302544.364183/94‑1 (MQ=60)
                                                 GGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCT                                            >  SRR4302544.795452/1‑94 (MQ=60)
                                                    GTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGA                                         <  SRR4302544.3238415/94‑1 (MQ=60)
                                                     TTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAA                                        >  SRR4302544.1784765/1‑94 (MQ=60)
                                                     TTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAA                                        <  SRR4302544.3001203/94‑1 (MQ=60)
                                                       CTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAAT                                      <  SRR4302544.3999429/94‑1 (MQ=60)
                                                            GCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTA                                 >  SRR4302544.1713334/1‑94 (MQ=60)
                                                             CTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAA                                <  SRR4302544.975998/94‑1 (MQ=60)
                                                             CTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAA                                <  SRR4302544.426429/94‑1 (MQ=60)
                                                             CTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAA                                >  SRR4302544.3298968/1‑94 (MQ=60)
                                                                CTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGA                             >  SRR4302544.360391/1‑94 (MQ=60)
                                                                  GCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACG                           <  SRR4302544.309067/94‑1 (MQ=60)
                                                                     GCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATG                        <  SRR4302544.1613599/94‑1 (MQ=60)
                                                                      CAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGG                       >  SRR4302544.3454568/1‑94 (MQ=60)
                                                                      CAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGG                       <  SRR4302544.3859362/94‑1 (MQ=60)
                                                                       AGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGT                      >  SRR4302544.1369539/1‑94 (MQ=60)
                                                                       AGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGA                      >  SRR4302544.3849357/1‑94 (MQ=60)
                                                                       AGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGA                      >  SRR4302544.3849356/1‑94 (MQ=60)
                                                                        GGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTA                     <  SRR4302544.2737887/94‑1 (MQ=60)
                                                                          CGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAAT                   <  SRR4302544.3027336/94‑1 (MQ=60)
                                                                           GCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATA                  >  SRR4302544.4682550/1‑94 (MQ=60)
                                                                            CTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATAC                 <  SRR4302544.3506883/94‑1 (MQ=60)
                                                                            CTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATAC                 <  SRR4302544.1395116/94‑1 (MQ=60)
                                                                            CTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATAC                 <  SRR4302544.1274728/94‑1 (MQ=60)
                                                                            CTGTGGCAGGTGGCGTAGCTGGTGAGGGCGTACAGACTGGAATGAACAAAACGCAGGGTGTCGAGCTGCAAATTCGTAAAGACGATGGTAATAC                 <  SRR4302544.4789401/94‑1 (MQ=60)
                                                                               TTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCAT              <  SRR4302544.466520/94‑1 (MQ=60)
                                                                                TGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAAGGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATC             >  SRR4302544.821935/1‑94 (MQ=60)
                                                                                TGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATC             >  SRR4302544.821785/1‑94 (MQ=60)
                                                                                TGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATC             <  SRR4302544.4082901/94‑1 (MQ=60)
                                                                                  CAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAT           <  SRR4302544.2169146/94‑1 (MQ=60)
                                                                                   AGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATG          <  SRR4302544.3443511/94‑1 (MQ=60)
                                                                                      TGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTG       >  SRR4302544.4360900/1‑94 (MQ=60)
                                                                                           TAGCTGGTCAGGGCGTGCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACA  >  SRR4302544.4669531/1‑94 (MQ=60)
                                                                                           TAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACA  <  SRR4302544.1911369/94‑1 (MQ=60)
                                                                                             |                                                                                           
GGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAACTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACA  >  NC_000913/1718098‑1718282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑