Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,718,191 A→G T98A (ACT→GCT)  slyB → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,718,1910AG100.0% 732.5 / NA 224T98A (ACT→GCT) slyBputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (110/114);  total (110/114)

Only 100 of 229 total aligned reads displayed.

GCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAACTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTA  >  NC_000913/1718101‑1718280
                                                                                          |                                                                                         
gCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCt                                                                                         <  2:2802616/93‑1 (MQ=255)
 cTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTg                                                                                        >  2:2317453/1‑93 (MQ=255)
  tGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACAGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTgg                                                                                       >  2:3569385/1‑93 (MQ=255)
    ttCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTc                                                                                     >  1:5268613/1‑93 (MQ=255)
     tCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCa                                                                                    <  2:4675460/93‑1 (MQ=255)
      cTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAg                                                                                   <  1:5591563/93‑1 (MQ=255)
       ttGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAgg                                                                                  >  1:5860160/1‑93 (MQ=255)
         ggtggtTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGc                                                                                <  1:375296/93‑1 (MQ=255)
          gtggtTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCg                                                                               >  1:1644226/1‑93 (MQ=255)
            ggtTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTa                                                                             <  2:5505976/93‑1 (MQ=255)
            ggtTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTa                                                                             >  2:4184637/1‑93 (MQ=255)
               ttCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACag                                                                          <  1:5636503/93‑1 (MQ=255)
                 ccTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACagag                                                                        <  1:3685454/93‑1 (MQ=255)
                  cTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGt                                                                       <  1:2684338/93‑1 (MQ=255)
                  cTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGt                                                                       <  1:4957150/93‑1 (MQ=255)
                   tGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTg                                                                      >  2:1662992/1‑93 (MQ=255)
                    ggggAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGc                                                                     <  2:6488859/93‑1 (MQ=255)
                     gggAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCa                                                                    >  2:2939611/1‑93 (MQ=255)
                      ggAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCaa                                                                   <  2:5781243/93‑1 (MQ=255)
                        aaTACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATg                                                                 <  1:3152811/93‑1 (MQ=255)
                         aTACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGa                                                                >  1:2400576/1‑93 (MQ=255)
                         aTACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGa                                                                >  2:3696107/1‑93 (MQ=255)
                          tACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGaa                                                               <  2:654390/93‑1 (MQ=255)
                           aCTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAAc                                                              >  2:3313445/1‑93 (MQ=255)
                            cTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACa                                                             >  2:1149644/1‑93 (MQ=255)
                              gTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACaaa                                                           <  1:6469728/93‑1 (MQ=255)
                               ttGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACaaaa                                                          <  2:1908787/93‑1 (MQ=255)
                               ttGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACaaaa                                                          <  1:5933873/93‑1 (MQ=255)
                                   tggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCa                                                      <  2:3157966/93‑1 (MQ=255)
                                   tggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCa                                                      >  1:2112061/1‑93 (MQ=255)
                                   gggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCa                                                      <  2:4944921/92‑1 (MQ=255)
                                     gCGGAGCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAgg                                                    <  2:693845/93‑1 (MQ=255)
                                     gCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAgg                                                    <  1:4429323/93‑1 (MQ=255)
                                     gCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAgg                                                    >  2:1281105/1‑93 (MQ=255)
                                      cGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAggg                                                   <  1:1011742/93‑1 (MQ=255)
                                      cGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAggg                                                   <  1:5494484/93‑1 (MQ=255)
                                       ggAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAgggg                                                  >  1:3055828/1‑92 (MQ=255)
                                        gAACCGGGCGTTCTCTGGCTACTGCAGCAGGTGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCGATGAACAAAACGCAgggcg                                                 >  1:1779048/1‑91 (MQ=255)
                                        gAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGgtg                                                 <  1:1074905/93‑1 (MQ=255)
                                        gAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGgtg                                                 <  2:6387767/93‑1 (MQ=255)
                                         aaCCGGGCGTTCTTTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGgtgt                                                <  2:4116705/93‑1 (MQ=255)
                                         aaCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGgtgt                                                >  2:511167/1‑93 (MQ=255)
                                          aCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTc                                               >  2:2499086/1‑93 (MQ=255)
                                           ccGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCg                                              >  1:5024541/1‑93 (MQ=255)
                                            cGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGa                                             <  1:1177469/93‑1 (MQ=255)
                                             gggCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAg                                            >  1:4884761/1‑93 (MQ=255)
                                             gggCGTTCTCTGGCTACTGCAGCAGGAGCTGTTGCAGGTGGCGTGGCTGGTCAGGGCGTACAGAGGGGAATGAACAAAACGCAGGGGGTCGAg                                            >  1:1838096/1‑93 (MQ=255)
                                              ggCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGc                                           >  2:6500759/1‑93 (MQ=255)
                                               gCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCt                                          <  2:5795230/93‑1 (MQ=255)
                                               gCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCt                                          <  1:1669904/93‑1 (MQ=255)
                                                 gTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTATAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTgg                                        >  2:2040945/1‑93 (MQ=255)
                                                 gTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTgg                                        >  1:3356574/1‑93 (MQ=255)
                                                 gTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTgg                                        >  2:175589/1‑93 (MQ=255)
                                                 gTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGGGTCGAGCTgg                                        >  1:4632370/1‑93 (MQ=255)
                                                  ttCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGa                                       >  1:6095803/1‑93 (MQ=255)
                                                   tctcTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGaa                                      >  1:2223944/1‑93 (MQ=255)
                                                    ctctGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGGGCAATGAACAAAACGCAGGGTGTCGAGCTGGaaa                                     >  2:160640/1‑93 (MQ=255)
                                                     tctGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAAt                                    <  2:2767847/93‑1 (MQ=255)
                                                         gCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGt                                >  2:5901760/1‑93 (MQ=255)
                                                          cTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTa                               >  1:3204585/1‑93 (MQ=255)
                                                          cTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTa                               <  1:2825197/93‑1 (MQ=255)
                                                            aCTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTaaa                             <  1:184534/93‑1 (MQ=255)
                                                              tGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCTGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGa                           >  2:5835499/1‑93 (MQ=255)
                                                              tGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGa                           <  1:6577235/93‑1 (MQ=255)
                                                               gcagcaGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGAc                          <  2:734943/93‑1 (MQ=255)
                                                               gcagcaGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGAc                          <  2:4566791/93‑1 (MQ=255)
                                                               gcagcaGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGAc                          >  2:592239/1‑93 (MQ=255)
                                                               gcagcaGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGAc                          >  1:4718889/1‑93 (MQ=255)
                                                                 agcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGa                        >  1:4701794/1‑93 (MQ=255)
                                                                 agcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGa                        >  1:5037420/1‑93 (MQ=255)
                                                                  gcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGAt                       <  2:1385428/93‑1 (MQ=255)
                                                                  gcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGAt                       >  2:4492586/1‑93 (MQ=255)
                                                                  gcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGAt                       >  1:4140852/1‑93 (MQ=255)
                                                                  gcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGAt                       <  2:1256484/93‑1 (MQ=255)
                                                                    agGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATgg                     <  1:3399027/93‑1 (MQ=255)
                                                                    agGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATgg                     <  2:4303513/93‑1 (MQ=255)
                                                                    agGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATgg                     >  1:1873495/1‑93 (MQ=255)
                                                                    agGCGCTGTGGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATgg                     <  1:1693002/93‑1 (MQ=255)
                                                                     ggCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATgga                    >  2:6007029/1‑92 (MQ=255)
                                                                     ggCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGt                    >  2:5246157/1‑93 (MQ=255)
                                                                     ggCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGt                    >  1:5717306/1‑93 (MQ=255)
                                                                       cgcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTaa                  >  2:4379319/1‑93 (MQ=255)
                                                                        gcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAACCGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAAt                 <  1:691234/93‑1 (MQ=255)
                                                                        gcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAAt                 >  1:3425570/1‑93 (MQ=255)
                                                                        gcTGTTGCAGGTGGCGTAGCTGGTCAGGCCGTACAGAGTGCAATGAACAAATCGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAAt                 <  1:621724/93‑1 (MQ=255)
                                                                           gTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATAcc              >  2:795850/1‑93 (MQ=255)
                                                                           gTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATAcc              <  1:2519226/93‑1 (MQ=255)
                                                                            ttGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACca             >  2:4107815/1‑93 (MQ=255)
                                                                              gCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACcatc           >  2:3299107/1‑93 (MQ=255)
                                                                               cAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATAtcatca          <  1:4220426/93‑1 (MQ=255)
                                                                                 ggTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAtg        >  2:6066329/1‑93 (MQ=255)
                                                                                 ggTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGCGTCGAGCTGGAAATCCGAAAGAACAAGGGAAAACCCATCAtg        >  2:3836326/1‑93 (MQ=255)
                                                                                  gTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAACTTCGTAAAGACGATGGTAATACCATCAtgg       >  1:3312127/1‑93 (MQ=255)
                                                                                   tGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAtggt      <  1:6344165/93‑1 (MQ=255)
                                                                                     gCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAtggtgg    >  2:10466/1‑93 (MQ=255)
                                                                                      cGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATggtggt   >  2:3761454/1‑93 (MQ=255)
                                                                                       gTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTa  >  1:759496/1‑93 (MQ=255)
                                                                                       gTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTa  >  2:642316/1‑93 (MQ=255)
                                                                                       gTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTa  >  1:2316101/1‑93 (MQ=255)
                                                                                       gTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTa  >  1:1285802/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAACTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTA  >  NC_000913/1718101‑1718280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAACTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAG  >  NC_000913/1718098‑1718283
                                                                                             |                                                                                            
GGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGACCCGGGCGTCCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAG                                                                                              <  SRR4302536.5557103/94‑1 (MQ=60)
GGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAG                                                                                              <  SRR4302536.15093/94‑1 (MQ=60)
GGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAG                                                                                              <  SRR4302536.3270838/94‑1 (MQ=60)
GGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAC                                                                                              >  SRR4302536.5093030/1‑94 (MQ=60)
  TGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCT                                                                                            <  SRR4302536.4304273/94‑1 (MQ=60)
    CTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGG                                                                                          >  SRR4302536.1171625/1‑94 (MQ=60)
     TGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGT                                                                                         <  SRR4302536.2208631/94‑1 (MQ=60)
      GTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTC                                                                                        >  SRR4302536.5425639/1‑94 (MQ=60)
      GTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTC                                                                                        >  SRR4302536.608910/1‑94 (MQ=60)
       TTGTTGGTGGTTTCCTGGGGAATCCTGTTGGTGGAGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCA                                                                                       <  SRR4302536.3843876/94‑1 (MQ=60)
         CTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGG                                                                                     <  SRR4302536.5757839/94‑1 (MQ=60)
         CTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGG                                                                                     >  SRR4302536.6034747/1‑94 (MQ=60)
           TGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGC                                                                                   <  SRR4302536.1822539/94‑1 (MQ=60)
            GGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCG                                                                                  >  SRR4302536.1692054/1‑94 (MQ=60)
            GGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCG                                                                                  <  SRR4302536.385185/94‑1 (MQ=60)
              TGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTA                                                                                <  SRR4302536.1782488/94‑1 (MQ=60)
               GGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTAC                                                                               <  SRR4302536.2857478/94‑1 (MQ=60)
                 TTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAG                                                                             >  SRR4302536.5173829/1‑94 (MQ=60)
                  TTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGA                                                                            <  SRR4302536.5803947/94‑1 (MQ=60)
                   TCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAG                                                                           <  SRR4302536.6903255/94‑1 (MQ=60)
                   TCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAG                                                                           >  SRR4302536.4308715/1‑94 (MQ=60)
                    CCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGT                                                                          <  SRR4302536.3795631/94‑1 (MQ=60)
                    CCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGT                                                                          >  SRR4302536.1912197/1‑94 (MQ=60)
                    CCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGT                                                                          <  SRR4302536.1848033/94‑1 (MQ=60)
                     CTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTG                                                                         <  SRR4302536.2764522/94‑1 (MQ=60)
                     CTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTG                                                                         <  SRR4302536.5223735/94‑1 (MQ=60)
                     CTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTG                                                                         <  SRR4302536.5106103/94‑1 (MQ=60)
                      TGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGC                                                                        >  SRR4302536.3318794/1‑94 (MQ=60)
                        GGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAA                                                                      >  SRR4302536.1737094/1‑94 (MQ=60)
                           AATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGA                                                                   >  SRR4302536.2473228/1‑94 (MQ=60)
                           AATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGA                                                                   <  SRR4302536.3245139/94‑1 (MQ=60)
                             TACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGAGCAATGAAC                                                                 >  SRR4302536.104351/1‑94 (MQ=60)
                              ACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACA                                                                <  SRR4302536.4869429/94‑1 (MQ=60)
                              ACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACA                                                                >  SRR4302536.4835311/1‑94 (MQ=60)
                               CTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAA                                                               >  SRR4302536.4844207/1‑94 (MQ=60)
                               CTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAA                                                               <  SRR4302536.547338/94‑1 (MQ=60)
                                 GTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAA                                                             <  SRR4302536.6662828/94‑1 (MQ=60)
                                  TTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAAC                                                            <  SRR4302536.3779986/94‑1 (MQ=60)
                                  TTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAAC                                                            <  SRR4302536.3255535/94‑1 (MQ=60)
                                  TTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAAC                                                            >  SRR4302536.1985884/1‑94 (MQ=60)
                                  TTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAAC                                                            <  SRR4302536.6110625/94‑1 (MQ=60)
                                   TGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACG                                                           <  SRR4302536.2967642/94‑1 (MQ=60)
                                     GTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCA                                                         >  SRR4302536.2175530/1‑94 (MQ=60)
                                       GGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGG                                                       <  SRR4302536.4272681/94‑1 (MQ=60)
                                       GGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGG                                                       <  SRR4302536.3463089/94‑1 (MQ=60)
                                       GGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGG                                                       >  SRR4302536.505928/1‑94 (MQ=60)
                                        GCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGG                                                      <  SRR4302536.4562076/94‑1 (MQ=60)
                                        GCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGG                                                      <  SRR4302536.890415/94‑1 (MQ=60)
                                         CGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGT                                                     <  SRR4302536.1041605/94‑1 (MQ=60)
                                         CGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGT                                                     <  SRR4302536.5658457/94‑1 (MQ=60)
                                         CGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGT                                                     <  SRR4302536.3523628/94‑1 (MQ=60)
                                         CGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGG                                                     >  SRR4302536.3145559/1‑94 (MQ=60)
                                          GGAGCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTG                                                    <  SRR4302536.3148135/94‑1 (MQ=60)
                                          GGAACCGGGCGTTCTCTGGCTACTGCAGCAGGTGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCGATGAACAAAACGCAGGGCG                                                    >  SRR4302536.1832837/1‑94 (MQ=60)
                                          GGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTG                                                    <  SRR4302536.2647055/94‑1 (MQ=60)
                                          GGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTG                                                    >  SRR4302536.2406639/1‑94 (MQ=60)
                                           GAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGT                                                   <  SRR4302536.1107078/94‑1 (MQ=60)
                                             ACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCG                                                 >  SRR4302536.5175148/1‑94 (MQ=60)
                                             ACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCG                                                 >  SRR4302536.1715430/1‑94 (MQ=60)
                                              CCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGA                                                <  SRR4302536.6286283/94‑1 (MQ=60)
                                               CGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAG                                               >  SRR4302536.5031703/1‑94 (MQ=60)
                                               CGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAG                                               <  SRR4302536.1212918/94‑1 (MQ=60)
                                               CGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAG                                               >  SRR4302536.5475317/1‑94 (MQ=60)
                                               CGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAG                                               >  SRR4302536.3299507/1‑94 (MQ=60)
                                               CGGGCGTTCTCTGGCTACTGCAGCAGGAGCTGTTGCAGGTGGCGTGGCTGGTCAGGGCGTACAGAGGGGAATGAACAAAACGCAGGGGGTCGAG                                               >  SRR4302536.1893523/1‑94 (MQ=60)
                                                  GCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTG                                            <  SRR4302536.1718341/94‑1 (MQ=60)
                                                   CGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCATGGCGTACAGAGTGCAATtatcaaaaagcagagcgtcgaggctg                                           >  SRR4302536.2398181/1‑68 (MQ=60)
                                                   CGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGG                                           >  SRR4302536.4858997/1‑94 (MQ=60)
                                                   CGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGG                                           >  SRR4302536.3456203/1‑94 (MQ=60)
                                                   CGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGGGTCGAGCTGG                                           >  SRR4302536.4771585/1‑94 (MQ=60)
                                                    GTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGA                                          >  SRR4302536.6277555/1‑94 (MQ=60)
                                                     TTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGTGTGTCGAGCTGGAA                                         >  SRR4302536.4427568/1‑94 (MQ=60)
                                                     TTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAA                                         >  SRR4302536.2291213/1‑94 (MQ=60)
                                                         CTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTC                                     >  SRR4302536.3617162/1‑94 (MQ=60)
                                                         CTGGCTACTGCAGCAGGCGCTGTTGCAGGGGGCGTAGCTGGGCAGGGCGAACAGAGTGCAAGGAACAAAACGCAGGGTGACGAGCgggaagttg                                     >  SRR4302536.2001536/1‑85 (MQ=60)
                                                            GCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTA                                  >  SRR4302536.6618721/1‑94 (MQ=60)
                                                            GCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTA                                  >  SRR4302536.3298577/1‑94 (MQ=60)
                                                             CTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAA                                 <  SRR4302536.2908834/94‑1 (MQ=60)
                                                             CTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAA                                 >  SRR4302536.3425236/1‑94 (MQ=60)
                                                              TACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAA                                <  SRR4302536.2306804/94‑1 (MQ=60)
                                                               ACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAG                               >  SRR4302536.5178060/1‑94 (MQ=60)
                                                               ACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAG                               <  SRR4302536.6258703/94‑1 (MQ=60)
                                                               ACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAG                               <  SRR4302536.189404/94‑1 (MQ=60)
                                                                CTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGA                              >  SRR4302536.2395917/1‑94 (MQ=60)
                                                                 TGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGAC                             >  SRR4302536.4860862/1‑94 (MQ=60)
                                                                 TGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGAC                             <  SRR4302536.6772979/94‑1 (MQ=60)
                                                                 TGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGAC                             <  SRR4302536.1382908/94‑1 (MQ=60)
                                                                   CAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGA                           >  SRR4302536.4843173/1‑94 (MQ=60)
                                                                   CAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGA                           >  SRR4302536.2536483/1‑94 (MQ=60)
                                                                   CAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGA                           >  SRR4302536.5188432/1‑94 (MQ=60)
                                                                    AGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGAT                          >  SRR4302536.4263681/1‑94 (MQ=60)
                                                                     GCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATG                         <  SRR4302536.5173398/94‑1 (MQ=60)
                                                                     GCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATG                         <  SRR4302536.2113655/94‑1 (MQ=60)
                                                                      CAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGG                        >  SRR4302536.1929887/1‑94 (MQ=60)
                                                                      CAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGG                        >  SRR4302536.5716001/1‑94 (MQ=60)
                                                                      CAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGG                        >  SRR4302536.5818019/1‑94 (MQ=60)
                                                                       AGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGT                       >  SRR4302536.5887324/1‑94 (MQ=60)
                                                                       AGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGT                       <  SRR4302536.3500123/94‑1 (MQ=60)
                                                                       AGGCGCTGTGGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGT                       <  SRR4302536.1742017/94‑1 (MQ=60)
                                                                        GGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTA                      <  SRR4302536.1213814/94‑1 (MQ=60)
                                                                         GCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAA                     <  SRR4302536.1899831/94‑1 (MQ=60)
                                                                         GCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAA                     <  SRR4302536.4757569/94‑1 (MQ=60)
                                                                          CGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAGTTCGTAAAGACGATGGTAAT                    <  SRR4302536.6712233/94‑1 (MQ=60)
                                                                          CGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAAT                    >  SRR4302536.3527527/1‑94 (MQ=60)
                                                                          CGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAAT                    >  SRR4302536.157905/1‑94 (MQ=60)
                                                                           GCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAACCGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATA                   <  SRR4302536.711444/94‑1 (MQ=60)
                                                                           GCTGTTGCAGGTGGCGTAGCTGGTCAGGCCGTACAGAGTGCAATGAACAAATCGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATA                   <  SRR4302536.640189/94‑1 (MQ=60)
                                                                              GTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGGAATTCGTAAAGACGATGGTAATACCA                >  SRR4302536.746679/1‑94 (MQ=60)
                                                                              GTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCA                <  SRR4302536.2595177/94‑1 (MQ=60)
                                                                                  CAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATATCATCAT            <  SRR4302536.4345984/94‑1 (MQ=60)
                                                                                  CAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAT            <  SRR4302536.2988797/94‑1 (MQ=60)
                                                                                  CAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAT            <  SRR4302536.6431707/94‑1 (MQ=60)
                                                                                   AGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATG           <  SRR4302536.5981924/94‑1 (MQ=60)
                                                                                   AGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATG           <  SRR4302536.684580/94‑1 (MQ=60)
                                                                                    GGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGTGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGG          <  SRR4302536.6379589/94‑1 (MQ=60)
                                                                                    GGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAACTTCGTAAAGACGATGGTAATACCATCATGG          >  SRR4302536.3409990/1‑94 (MQ=60)
                                                                                    GGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGG          <  SRR4302536.5920507/94‑1 (MQ=60)
                                                                                      TGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTG        <  SRR4302536.5784573/94‑1 (MQ=60)
                                                                                      TGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTG        <  SRR4302536.6533173/94‑1 (MQ=60)
                                                                                      TGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTG        <  SRR4302536.4429032/94‑1 (MQ=60)
                                                                                       GGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGG       <  SRR4302536.4244788/94‑1 (MQ=60)
                                                                                         CGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTA     >  SRR4302536.1324274/1‑94 (MQ=60)
                                                                                         CGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTA     >  SRR4302536.2386391/1‑94 (MQ=60)
                                                                                         CGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTA     >  SRR4302536.781767/1‑94 (MQ=60)
                                                                                          GTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTAC    >  SRR4302536.675841/1‑94 (MQ=60)
                                                                                          GTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTAC    >  SRR4302536.504933/1‑94 (MQ=60)
                                                                                           TAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACA   >  SRR4302536.4113555/1‑94 (MQ=60)
                                                                                           TAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACA   >  SRR4302536.4532669/1‑94 (MQ=60)
                                                                                            AGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAG  <  SRR4302536.5619063/94‑1 (MQ=60)
                                                                                            AGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAG  >  SRR4302536.4884421/1‑94 (MQ=60)
                                                                                            AGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAG  >  SRR4302536.3373202/1‑94 (MQ=60)
                                                                                             |                                                                                            
GGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAACTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAG  >  NC_000913/1718098‑1718283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑