Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,729,644 T→C M845T (ATG→ACG)  lhr → Lhr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,729,6440TC97.4% 479.6 / ‑1.4 155M845T (ATG→ACG) lhrLhr
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base C (79/71);  total (82/73)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

CTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTAT  >  NC_000913/1729554‑1729732
                                                                                          |                                                                                        
cTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGc                                                                                        >  1:2494670/1‑93 (MQ=255)
cTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGc                                                                                        <  1:758796/93‑1 (MQ=255)
 tCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGCTGTTTATGATCTTCCCGCACTGGTCGGTTTGAcgcg                                                                                       >  2:939562/1‑93 (MQ=255)
 tCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGAcgcg                                                                                       >  1:1867934/1‑93 (MQ=255)
  cAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGt                                                                                      <  1:4390149/93‑1 (MQ=255)
   aGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTc                                                                                     >  1:4070603/1‑93 (MQ=255)
    gggATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCCGCAAATTATTTATAATCTTCCCGCCCTGGAACGTTTGACGCGTCg                                                                                    >  2:3488296/1‑93 (MQ=255)
        tatCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTg                                                                                <  1:4460475/93‑1 (MQ=255)
          tCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGaa                                                                              <  2:300080/93‑1 (MQ=255)
          tCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGaa                                                                              >  2:4479354/1‑93 (MQ=255)
          tCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGaa                                                                              <  2:4317657/93‑1 (MQ=255)
           ccGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAAc                                                                             <  1:3553316/93‑1 (MQ=255)
            cGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACg                                                                            >  2:2611089/1‑93 (MQ=255)
             ggATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACgg                                                                           <  2:3467197/93‑1 (MQ=255)
               aTTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTg                                                                         <  2:1584508/93‑1 (MQ=255)
                ttttCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTgg                                                                        <  2:448571/93‑1 (MQ=255)
                 tttCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACggcggc                                                                       >  1:1422052/1‑93 (MQ=255)
                 tttCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGc                                                                       <  2:198114/93‑1 (MQ=255)
                 tttCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGc                                                                       <  2:3727241/93‑1 (MQ=255)
                  ttCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCg                                                                      >  2:1228525/1‑93 (MQ=255)
                    ccGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGaa                                                                    >  2:1452071/1‑93 (MQ=255)
                     cGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGANGNNTCGCCTGAACGGTGGCGaca                                                                   >  1:1606253/1‑91 (MQ=255)
                     cGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGaaa                                                                   >  1:4310890/1‑93 (MQ=255)
                      ggTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAAt                                                                  >  1:560338/1‑93 (MQ=255)
                       gTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAAtt                                                                 >  1:3152712/1‑93 (MQ=255)
                       gTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAAtt                                                                 >  1:3152713/1‑93 (MQ=255)
                        tCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTc                                                                <  2:1083542/93‑1 (MQ=255)
                         cATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCa                                                               >  1:2018353/1‑93 (MQ=255)
                         cATTCTCGAAACCCTACGCGAAGGTCTGCAAGAAGTTTATGATCTTCCCGCACTGGGACGTTTGTCGCGTCGCCTGAACGGTGGGGAAATTCa                                                               >  2:1035572/1‑93 (MQ=255)
                          aTTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAACTTCaa                                                              >  2:1513329/1‑93 (MQ=255)
                          aTTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCaa                                                              >  2:2285140/1‑93 (MQ=255)
                           ttCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCaaa                                                             >  2:3799909/1‑93 (MQ=255)
                            tctcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAat                                                            <  1:2008802/93‑1 (MQ=255)
                            tctcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGAAGCGTCGCCTGAACGGTGGCGAAATTCAAat                                                            <  1:3124044/93‑1 (MQ=255)
                                  aaCCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGa                                                      >  2:4657648/1‑93 (MQ=255)
                                  aaCCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGa                                                      >  2:4244197/1‑93 (MQ=255)
                                    cccTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATg                                                    >  1:1976639/1‑93 (MQ=255)
                                    cccTACGCGAATGGCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGCACGGTGGCGAAATTCAACTATCCGaat                                                    >  1:3305158/1‑91 (MQ=255)
                                     ccTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGt                                                   >  1:3562248/1‑93 (MQ=255)
                                        aCGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAAc                                                <  2:4286646/93‑1 (MQ=255)
                                         cgcgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACg                                               <  2:2149135/93‑1 (MQ=255)
                                          gcgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGa                                              >  2:3961240/1‑93 (MQ=255)
                                          gcgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGa                                              <  1:1575712/93‑1 (MQ=255)
                                          gcgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGa                                              <  1:4243357/93‑1 (MQ=255)
                                           cgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAc                                             >  1:2704416/1‑93 (MQ=255)
                                            gAATGTCTGCAAGATGTTTATGATCTTCCCGCCCTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAcc                                            >  1:3667545/1‑93 (MQ=255)
                                            gAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAcc                                            >  1:338601/1‑93 (MQ=255)
                                            gAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAcc                                            >  1:2476253/1‑93 (MQ=255)
                                            gAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAcc                                            <  1:3820666/93‑1 (MQ=255)
                                             aaagTCTGCAAGATGTATATGATCTTCCCGCACTGGAACGTTTGATGCGACGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCa                                           >  2:4214977/4‑93 (MQ=255)
                                             aaTGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCTGATGTAACGACCa                                           >  2:1247646/1‑93 (MQ=255)
                                              aTGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAacac                                          >  2:2026969/1‑93 (MQ=255)
                                              aTGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCAc                                          <  1:4720238/93‑1 (MQ=255)
                                               tGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACt                                         >  1:1924556/1‑93 (MQ=255)
                                                gtttGCTAGATGTTTATGATCTTCCCGCACTGGAACGTCTGACGCGTCGCCTGTACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTa                                        <  2:4087201/90‑1 (MQ=255)
                                                gTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTa                                        <  1:2659616/93‑1 (MQ=255)
                                                 tCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTAc                                       <  1:3707103/93‑1 (MQ=255)
                                                 tCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAAAGACGACTAc                                       >  1:98180/1‑93 (MQ=255)
                                                   tGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGc                                     >  2:4350664/1‑93 (MQ=255)
                                                    gCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCTGATGTAACGACCACTACGcc                                    >  1:2676439/1‑93 (MQ=255)
                                                     cAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGccc                                   >  1:1481593/1‑93 (MQ=255)
                                                      aaGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGTACGGTGGCGAAATTCAAATATCCGATGTACCGACCACTACGcccc                                  >  2:622534/1‑92 (MQ=255)
                                                      aaGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCt                                  >  1:1244751/1‑93 (MQ=255)
                                                      aaGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCt                                  >  2:2386268/1‑93 (MQ=255)
                                                       aGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGATCGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTc                                 <  2:1647932/93‑1 (MQ=255)
                                                       aGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTc                                 >  1:1064643/1‑93 (MQ=255)
                                                        gATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCg                                >  2:3239364/1‑93 (MQ=255)
                                                         aTGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGc                               >  1:3012757/1‑93 (MQ=255)
                                                            tttATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCtt                            <  1:4213947/93‑1 (MQ=255)
                                                            tttATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCtt                            <  1:1181744/93‑1 (MQ=255)
                                                            tttATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCtt                            >  2:1169875/1‑93 (MQ=255)
                                                                tGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGc                        <  2:2840970/93‑1 (MQ=255)
                                                                 gATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGcc                       >  1:3466626/1‑93 (MQ=255)
                                                                 gATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGcc                       >  1:447198/1‑93 (MQ=255)
                                                                   tCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCcac                     <  1:129126/93‑1 (MQ=255)
                                                                    cTTCCCGCACTGGAACGTTTGACGCGTCGCCTGATCGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCcaca                    <  2:3939930/93‑1 (MQ=255)
                                                                     ttCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCTGATGTAACGACAACTACGCCCCCACTTTTCTCCACaa                   >  1:3725430/1‑93 (MQ=255)
                                                                     ttCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACaa                   <  1:1014965/93‑1 (MQ=255)
                                                                     ttCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACaa                   <  1:961832/93‑1 (MQ=255)
                                                                       cccGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGt                 <  2:2570323/93‑1 (MQ=255)
                                                                       cccGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGt                 >  1:713310/1‑93 (MQ=255)
                                                                        ccGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTACCGACCACTACGCCCTCGCCTTTCGCCACAAGtt                <  1:246257/93‑1 (MQ=255)
                                                                        ccGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGtt                >  2:1070392/1‑93 (MQ=255)
                                                                          gCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTa              <  2:3839471/93‑1 (MQ=255)
                                                                          gCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTa              >  2:3791168/1‑93 (MQ=255)
                                                                          gCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTa              >  1:3064184/1‑93 (MQ=255)
                                                                              tGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGt          >  1:3429100/1‑93 (MQ=255)
                                                                              tGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGt          <  2:824198/93‑1 (MQ=255)
                                                                               ggAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATcc                                                        <  1:2781510/46‑1 (MQ=255)
                                                                               ggAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATcc                                                        >  2:2781510/1‑46 (MQ=255)
                                                                                gAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAGCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTc        <  2:4064575/93‑1 (MQ=255)
                                                                                gAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTGCGCCCTCGCCTTTCGCCACAAGTTTATTGTTc        <  2:593305/93‑1 (MQ=255)
                                                                                gAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTc        <  1:4051466/93‑1 (MQ=255)
                                                                                   cGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGc     <  2:2258854/93‑1 (MQ=255)
                                                                                    gTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTTTTCGGCt    >  1:1508246/1‑93 (MQ=255)
                                                                                    gTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCt    <  1:181494/93‑1 (MQ=255)
                                                                                    gTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCt    >  1:292403/1‑93 (MQ=255)
                                                                                     tttGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTa   >  1:568157/1‑93 (MQ=255)
                                                                                     tttGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTa   <  1:1025071/93‑1 (MQ=255)
                                                                                      ttGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTAt  >  2:293599/1‑93 (MQ=255)
                                                                                          |                                                                                        
CTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTAT  >  NC_000913/1729554‑1729732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTAT  >  NC_000913/1729551‑1729732
                                                                                             |                                                                                        
TCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGAC                                                                                          <  SRR4302544.2904007/94‑1 (MQ=60)
  GCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGC                                                                                        <  SRR4302544.565483/94‑1 (MQ=60)
  GCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGC                                                                                        >  SRR4302544.2518574/1‑94 (MQ=60)
   CTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCG                                                                                       <  SRR4302544.766197/94‑1 (MQ=60)
   CTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCG                                                                                       >  SRR4302544.1885990/1‑94 (MQ=60)
     CAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTC                                                                                     >  SRR4302544.4111116/1‑94 (MQ=60)
     CAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTC                                                                                     >  SRR4302544.4111115/1‑94 (MQ=60)
     CAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTC                                                                                     <  SRR4302544.4434576/94‑1 (MQ=60)
     CAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTC                                                                                     >  SRR4302544.316637/1‑94 (MQ=60)
      AGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCC                                                                                    >  SRR4302544.3789665/1‑94 (MQ=60)
       GGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGC                                                                                   <  SRR4302544.1561477/94‑1 (MQ=60)
           TATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGA                                                                               <  SRR4302544.4505375/94‑1 (MQ=60)
           TATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGA                                                                               <  SRR4302544.2697595/94‑1 (MQ=60)
              CCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACG                                                                            <  SRR4302544.3587356/94‑1 (MQ=60)
                GGATTTTCCGTTCGTTCTCGAACCCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGT                                                                          <  SRR4302544.4267943/94‑1 (MQ=60)
                GGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGT                                                                          <  SRR4302544.1981607/94‑1 (MQ=60)
                GGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGT                                                                          <  SRR4302544.1996276/94‑1 (MQ=60)
                  ATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGG                                                                        >  SRR4302544.2902522/1‑94 (MQ=60)
                   TTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGCGGC                                                                       >  SRR4302544.1436206/1‑94 (MQ=60)
                       CCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGANGNNTCGCCTGAACGGTGGCGACA                                                                   >  SRR4302544.1622084/1‑94 (MQ=60)
                       CCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAA                                                                   >  SRR4302544.4354823/1‑94 (MQ=60)
                        CGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAAT                                                                  >  SRR4302544.565812/1‑94 (MQ=60)
                         GGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATT                                                                 >  SRR4302544.3182701/1‑94 (MQ=60)
                         GGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATT                                                                 >  SRR4302544.3182700/1‑94 (MQ=60)
                           TCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCA                                                               >  SRR4302544.2037952/1‑94 (MQ=60)
                           TCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCA                                                               <  SRR4302544.2489416/94‑1 (MQ=60)
                               TCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATA                                                           <  SRR4302544.2028319/94‑1 (MQ=60)
                               TCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGAAGCGTCGCCTGAACGGTGGCGAAATTCAAATA                                                           <  SRR4302544.3153740/94‑1 (MQ=60)
                                  CGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCC                                                        <  SRR4302544.364547/94‑1 (MQ=60)
                                   atcggtctcggcattcctgctgaaccgctcttccgatcttatatctTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCG                                                       <  SRR4302544.2808285/48‑1 (MQ=60)
                                      ACGCTACGCGAATGTCTGCAAGATGTTTATGATCTACCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATG                                                    <  SRR4302544.3995616/94‑1 (MQ=60)
                                      ACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATG                                                    >  SRR4302544.1995851/1‑94 (MQ=60)
                                      ACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATG                                                    >  SRR4302544.3336644/1‑94 (MQ=60)
                                      ACCCTACGCGAATGGCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGCACGGTGGCGAAATTCAACTATCCGAat                                                    >  SRR4302544.3336643/1‑92 (MQ=60)
                                       CCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGT                                                   >  SRR4302544.3596398/1‑94 (MQ=60)
                                         CTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAA                                                 <  SRR4302544.729591/94‑1 (MQ=60)
                                          TACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAAC                                                <  SRR4302544.1267084/94‑1 (MQ=60)
                                             GCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAC                                             <  SRR4302544.1591289/94‑1 (MQ=60)
                                             GCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAC                                             >  SRR4302544.2730418/1‑94 (MQ=60)
                                             GCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAC                                             <  SRR4302544.4286619/94‑1 (MQ=60)
                                              CGAATGTCTGCAAGATGTTTATGATCTTCCCGCCCTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACC                                            >  SRR4302544.3704119/1‑94 (MQ=60)
                                              CGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACC                                            >  SRR4302544.342112/1‑94 (MQ=60)
                                              CGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACC                                            >  SRR4302544.2499935/1‑94 (MQ=60)
                                               GAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCA                                           <  SRR4302544.3858538/94‑1 (MQ=60)
                                                 ATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT                                         <  SRR4302544.4652550/94‑1 (MQ=60)
                                                 ATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT                                         <  SRR4302544.4766845/94‑1 (MQ=60)
                                                 ATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACT                                         >  SRR4302544.1943172/1‑94 (MQ=60)
                                                   GTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTAC                                       <  SRR4302544.2685123/94‑1 (MQ=60)
                                                   GTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAAAGACGACTAC                                       >  SRR4302544.99078/1‑94 (MQ=60)
                                                    TCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACG                                      <  SRR4302544.3743989/94‑1 (MQ=60)
                                                     CTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGC                                     >  SRR4302544.3492813/1‑94 (MQ=60)
                                                      TGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCTGATGTAACGACCACTACGCC                                    >  SRR4302544.2702091/1‑94 (MQ=60)
                                                       GCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCC                                   >  SRR4302544.1496232/1‑94 (MQ=60)
                                                        CAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCT                                  <  SRR4302544.1862787/94‑1 (MQ=60)
                                                        CAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCT                                  >  SRR4302544.1257020/1‑94 (MQ=60)
                                                        CAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCC                                  >  SRR4302544.2354120/1‑94 (MQ=60)
                                                         AAGATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTC                                 >  SRR4302544.1075225/1‑94 (MQ=60)
                                                           GATGTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGC                               >  SRR4302544.3041640/1‑94 (MQ=60)
                                                              GTTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTT                            >  SRR4302544.2732645/1‑94 (MQ=60)
                                                               TTTATGATCTTCCCGCACTGGAACGTTTGACGGGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTT                           <  SRR4302544.3130012/94‑1 (MQ=60)
                                                               TTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTT                           <  SRR4302544.1193503/94‑1 (MQ=60)
                                                               TTTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTT                           <  SRR4302544.4256441/94‑1 (MQ=60)
                                                                TTATGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTC                          <  SRR4302544.1045704/94‑1 (MQ=60)
                                                                   TGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCC                       >  SRR4302544.3499693/1‑94 (MQ=60)
                                                                   TGATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCC                       >  SRR4302544.451651/1‑94 (MQ=60)
                                                                    GATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAAAACTACGCCCTCGCCTTTCGCCA                      <  SRR4302544.858908/94‑1 (MQ=60)
                                                                     ATCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCAC                     >  SRR4302544.4487620/1‑94 (MQ=60)
                                                                      TCTTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACA                    <  SRR4302544.130315/94‑1 (MQ=60)
                                                                       CTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCTGATGTAACGACAACTACGCCCCCACTTTTCTCCACAA                   >  SRR4302544.3762480/1‑94 (MQ=60)
                                                                        TTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAG                  <  SRR4302544.971278/94‑1 (MQ=60)
                                                                        TTCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAG                  <  SRR4302544.1025088/94‑1 (MQ=60)
                                                                         TCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGT                 >  SRR4302544.720376/1‑94 (MQ=60)
                                                                         TCCCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAAT                 <  SRR4302544.1774309/94‑1 (MQ=60)
                                                                           CCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTACCGACCACTACGCCCTCGCCTTTCGCCACAAGTTT               <  SRR4302544.248836/94‑1 (MQ=60)
                                                                           CCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTT               >  SRR4302544.3510325/1‑94 (MQ=60)
                                                                           CCGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTT               >  SRR4302544.492427/1‑94 (MQ=60)
                                                                            CGCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTA              >  SRR4302544.3093405/1‑94 (MQ=60)
                                                                             GCACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTAT             <  SRR4302544.1127487/94‑1 (MQ=60)
                                                                              CACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATT            <  SRR4302544.3027983/94‑1 (MQ=60)
                                                                               ACTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCATCGCCTTTCGCCACAAGTTTATTG           <  SRR4302544.571997/94‑1 (MQ=60)
                                                                                CTGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGT          >  SRR4302544.3461874/1‑94 (MQ=60)
                                                                                 TGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTT         >  SRR4302544.4425872/1‑94 (MQ=60)
                                                                                 TGGAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTT         >  SRR4302544.2564755/1‑94 (MQ=60)
                                                                                   GAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCG       <  SRR4302544.3245157/94‑1 (MQ=60)
                                                                                   GAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCG       <  SRR4302544.3737854/94‑1 (MQ=60)
                                                                                   GAACGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCG       <  SRR4302544.4091814/94‑1 (MQ=60)
                                                                                    AACGTTGGACGCGTCGCATGAACGGTGGCGAAATTCAAATATCCGATGTAGCGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGG      <  SRR4302544.614077/94‑1 (MQ=60)
                                                                                      CGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTTTTCGGCT    >  SRR4302544.1523198/1‑94 (MQ=60)
                                                                                      CGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCT    >  SRR4302544.295428/1‑94 (MQ=60)
                                                                                      CGTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGACCTCGCCTTTCGCCACAAGTTTATTGTTCGGCT    >  SRR4302544.1523197/1‑94 (MQ=60)
                                                                                       GTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCTATGTAACGACCACTACGCCCTCACCTTTCGCCACAAGTTTATTGTTCGGCTA   >  SRR4302544.1419757/1‑94 (MQ=60)
                                                                                       GTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTA   >  SRR4302544.573707/1‑94 (MQ=60)
                                                                                       GTTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTA   <  SRR4302544.183290/94‑1 (MQ=60)
                                                                                        TTTGACGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTAT  <  SRR4302544.1035304/94‑1 (MQ=60)
                                                                                             |                                                                                        
TCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTAT  >  NC_000913/1729551‑1729732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑