Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,731,175 T→C R1355R (CGT→CGC)  lhr → Lhr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,731,1750TC98.2% 333.3 / ‑2.5 108R1355R (CGT→CGC) lhrLhr
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (54/52);  total (55/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01

Only 100 of 115 total aligned reads displayed.

GTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCG  >  NC_000913/1731085‑1731264
                                                                                          |                                                                                         
gTCGTTATTTCAGGGTGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATTCTCGACCGCTGCGGCATCATCAGTCGCCa                                                                                         <  1:3670508/93‑1 (MQ=255)
gTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCa                                                                                         >  1:151645/1‑93 (MQ=255)
 tCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAg                                                                                        <  1:4487001/93‑1 (MQ=255)
 tCGTTATTGCAGATGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAg                                                                                        <  1:4487002/93‑1 (MQ=255)
 tCGTAATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAg                                                                                        <  2:4193945/93‑1 (MQ=255)
     tATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCgg                                                                                    >  2:2252677/1‑93 (MQ=255)
      aTTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGt                                                                                   <  2:3772962/93‑1 (MQ=255)
       ttGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTg                                                                                  >  2:1216655/1‑93 (MQ=255)
         gCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGAt                                                                                <  1:1397513/93‑1 (MQ=255)
          cAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATa                                                                               >  1:3336491/1‑93 (MQ=255)
           aGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCTCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAg                                                                              >  2:1434931/1‑93 (MQ=255)
            ggTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGc                                                                             <  2:1342134/93‑1 (MQ=255)
            ggTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGc                                                                             <  1:1231115/93‑1 (MQ=255)
             gTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGcc                                                                            >  1:508663/1‑93 (MQ=255)
             gTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGcc                                                                            >  1:508662/1‑93 (MQ=255)
             gTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGcc                                                                            >  1:2699302/1‑93 (MQ=255)
             gTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGcc                                                                            <  1:252005/93‑1 (MQ=255)
             gTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGCGATAGcc                                                                            <  2:229249/93‑1 (MQ=255)
              tGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGTCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCg                                                                           <  2:3136198/93‑1 (MQ=255)
              gggAGCCACTAAGGGATGCTGAATGGATGGTGGCGCGAGCGGAAAATGTGCTCGCCCGCTACGGCATCATCACTCGCCAGGCGGTGATAGCCg                                                                           <  2:4027676/92‑1 (MQ=255)
                gAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGaa                                                                         <  1:4610546/93‑1 (MQ=255)
                 aGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGaaa                                                                        >  2:1140332/1‑93 (MQ=255)
                  gCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGaaaa                                                                       >  1:2643624/1‑93 (MQ=255)
                  gCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGCGACAGCCGaaaa                                                                       >  2:1835226/1‑93 (MQ=255)
                  gCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCCGGGATAGCCGaaaa                                                                       >  1:1085475/1‑93 (MQ=255)
                    cACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGCAAata                                                                     >  1:2951218/1‑93 (MQ=255)
                    cACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAata                                                                     >  2:4548995/1‑93 (MQ=255)
                    cACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAAGCGGTGATAGCCGAAAata                                                                     >  2:2467655/1‑93 (MQ=255)
                      gtagaCGATACCGAAAGGATGCTGGCGGTGGCGGAAAATATGCTCGACCGCTACGGCATCATAAGTCGCCAGGCGGTGATAGCCGAAAATATc                                                                   <  2:339781/89‑1 (MQ=255)
                      cTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATc                                                                   <  1:4708464/93‑1 (MQ=255)
                       tAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATcc                                                                  <  1:1496738/93‑1 (MQ=255)
                       tAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATcc                                                                  >  1:3985169/1‑93 (MQ=255)
                       tAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAACATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATcc                                                                  >  2:1077706/1‑93 (MQ=255)
                        aaaCGATACCGAAAGGATGCTTGCGGTGGCGGAAAATATGCTCGACCGCTAGGGCATCAACCGTCGCCAGGCGGTGATAGCCGAAAATAGccc                                                                 <  2:1700047/93‑1 (MQ=255)
                        aaaCGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATccc                                                                 <  1:4126944/93‑1 (MQ=255)
                         aaCGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTCCGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCt                                                                >  1:1454426/1‑93 (MQ=255)
                         aaCGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCt                                                                >  1:1454427/1‑93 (MQ=255)
                         aaCGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCt                                                                >  1:4279313/1‑93 (MQ=255)
                         aaCGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCt                                                                >  1:1354293/1‑93 (MQ=255)
                         aaCGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCG‑AAATATCCCTg                                                               <  1:2106865/93‑1 (MQ=255)
                          aCGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGCCGGTGATAGCCGAAAATATCCCTg                                                               >  2:4039013/1‑93 (MQ=255)
                          aCGATACCCAAAGGATTCTGGCCCTCGCGGAAAATATGCTCGACCCCTACGGCATCATCAGTCGCCAGGCGCTCATAGCCGAAAATATCCCTg                                                               <  1:3632223/93‑1 (MQ=255)
                           cGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTgg                                                              <  1:4407083/93‑1 (MQ=255)
                            gATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGc                                                             >  1:3545734/1‑93 (MQ=255)
                            gATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGc                                                             >  1:1484178/1‑93 (MQ=255)
                            gATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGc                                                             >  2:2660115/1‑93 (MQ=255)
                             aTACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCg                                                            >  2:19563/1‑93 (MQ=255)
                             aTACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCAAAAATATCCCTGGCg                                                            <  1:1247376/93‑1 (MQ=255)
                                ccGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGt                                                         <  2:2779875/93‑1 (MQ=255)
                                  gAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGttt                                                       >  1:1103063/1‑93 (MQ=255)
                                   aaaGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTc                                                      >  1:445040/1‑93 (MQ=255)
                                    aaGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTcc                                                     >  1:4203158/1‑93 (MQ=255)
                                       gATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATc                                                  >  1:4677687/1‑93 (MQ=255)
                                          gctggcgctggcGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGAt                                               <  1:3693507/93‑1 (MQ=255)
                                               cgctggcgGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCaaa                                          <  1:3471318/93‑1 (MQ=255)
                                               cgctggcgGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCaaa                                          <  1:3603838/93‑1 (MQ=255)
                                               cgctggcgGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCGGGCGGGTTTCCATCGATGCaaa                                          <  2:4128492/93‑1 (MQ=255)
                                               cgctggcgGAAAAGATTCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCaaa                                          <  2:112983/93‑1 (MQ=255)
                                                gctggcgGAAAAAATGCTCGACCGCTACGGCATCATCAGTCGCAAGGCGGTGATAGCCGAAAATATCCCTGGCGGATTTCCATCGATGCAAAc                                         <  2:4210317/93‑1 (MQ=255)
                                                 ctggcgGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACg                                        >  1:4027150/1‑93 (MQ=255)
                                                    gcgGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCtt                                     <  1:4740637/93‑1 (MQ=255)
                                                     cgGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCttt                                    >  2:1515613/1‑93 (MQ=255)
                                                      ggAAAATATGCTCGACCGCTATGGCTTCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTTCATCGATGCAAACGCTCTg                                   >  2:4220493/1‑93 (MQ=255)
                                                       gAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGt                                  >  1:2792857/1‑93 (MQ=255)
                                                       gAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGt                                  <  1:2282610/93‑1 (MQ=255)
                                                        aaaaTATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTc                                 >  1:3934696/1‑93 (MQ=255)
                                                        aaaaTATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTc                                 <  2:4255011/93‑1 (MQ=255)
                                                        aaaaTATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTc                                 <  1:2864361/93‑1 (MQ=255)
                                                        aaaaTATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGGTTCCATCGATGCAAACGCTTTGTc                                 >  2:158448/1‑93 (MQ=255)
                                                         aaaTATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCg                                >  1:718593/1‑93 (MQ=255)
                                                          aaTATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGa                               <  2:446747/93‑1 (MQ=255)
                                                           atatGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGaa                              <  1:371856/93‑1 (MQ=255)
                                                            tatGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAg                             >  2:168831/1‑93 (MQ=255)
                                                              tGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTa                           >  2:1319607/1‑93 (MQ=255)
                                                              tGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTa                           >  1:2067170/1‑93 (MQ=255)
                                                                  cGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGa                       <  2:4627050/93‑1 (MQ=255)
                                                                  cGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGa                       >  1:2283807/1‑93 (MQ=255)
                                                                   gACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGaa                      <  1:2633931/93‑1 (MQ=255)
                                                                    aCCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGTGGGTTTCCATCGATGCAAACGTTTTGTCGAAGTATGGaaa                     >  1:4336456/1‑92 (MQ=255)
                                                                    aCCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGCTTCCATCGATGCAAACGCTTTGTCGACGTATGGAAg                     >  2:3906922/1‑93 (MQ=255)
                                                                      cGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGAc                   <  1:215101/93‑1 (MQ=255)
                                                                      cGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGAc                   <  1:1598081/93‑1 (MQ=255)
                                                                        cTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTc                 <  2:4035399/93‑1 (MQ=255)
                                                                         tACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTcc                <  1:330585/93‑1 (MQ=255)
                                                                         tACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTcc                >  1:744947/1‑93 (MQ=255)
                                                                         tACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTcc                <  2:3885363/93‑1 (MQ=255)
                                                                          aCGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCg               >  2:687789/1‑93 (MQ=255)
                                                                          aCGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCg               <  1:4509791/93‑1 (MQ=255)
                                                                          aCGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCg               >  1:3000788/1‑93 (MQ=255)
                                                                           cGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCgg              <  2:4700050/93‑1 (MQ=255)
                                                                           cGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCgg              <  2:75217/93‑1 (MQ=255)
                                                                             gCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGc            >  1:1181440/1‑93 (MQ=255)
                                                                                   tcaGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTa      <  2:313052/93‑1 (MQ=255)
                                                                                   tcaGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTa      >  1:3105722/1‑93 (MQ=255)
                                                                                   tcaGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTa      <  1:1443759/93‑1 (MQ=255)
                                                                                    caGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGGATGGACTAGTCCGGGCGAATTAt     >  2:1053858/1‑93 (MQ=255)
                                                                                     aGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATg    <  1:545421/93‑1 (MQ=255)
                                                                                     aGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATg    <  1:2815661/93‑1 (MQ=255)
                                                                                       tcgccAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCg  >  2:2109995/1‑93 (MQ=255)
                                                                                       tcgccAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCg  >  1:230575/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCG  >  NC_000913/1731085‑1731264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGGTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCGAGGT  >  NC_000913/1731082‑1731268
                                                                                             |                                                                                             
CTGGTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGC                                                                                               <  SRR4302544.2832176/94‑1 (MQ=60)
CTGGTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGC                                                                                               >  SRR4302544.2628187/1‑94 (MQ=60)
  GGTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCA                                                                                             <  SRR4302544.2585014/94‑1 (MQ=60)
  GGTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCA                                                                                             >  SRR4302544.153112/1‑94 (MQ=60)
   GTCGTTATTTCAGGGTGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATTCTCGACCGCTGCGGCATCATCAGTCGCCAG                                                                                            <  SRR4302544.3707117/94‑1 (MQ=60)
    TCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGG                                                                                           <  SRR4302544.4532074/94‑1 (MQ=60)
    TCGTTATTGCAGATGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGG                                                                                           <  SRR4302544.4532075/94‑1 (MQ=60)
         ATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTG                                                                                      <  SRR4302544.2461446/94‑1 (MQ=60)
            GCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATA                                                                                   <  SRR4302544.1411442/94‑1 (MQ=60)
            GCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATA                                                                                   >  SRR4302544.3368280/1‑94 (MQ=60)
               GGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCC                                                                                >  SRR4302544.2725240/1‑94 (MQ=60)
               GGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCC                                                                                >  SRR4302544.513680/1‑94 (MQ=60)
               GGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCC                                                                                >  SRR4302544.513679/1‑94 (MQ=60)
               GGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCC                                                                                <  SRR4302544.1243292/94‑1 (MQ=60)
                GTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCG                                                                               <  SRR4302544.3980172/94‑1 (MQ=60)
                GTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCG                                                                               <  SRR4302544.254652/94‑1 (MQ=60)
                   GAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAA                                                                            <  SRR4302544.4656335/94‑1 (MQ=60)
                    AGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAA                                                                           >  SRR4302544.2668970/1‑94 (MQ=60)
                    AGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCCGGGATAGCCGAAAA                                                                           >  SRR4302544.1096278/1‑94 (MQ=60)
                      CCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGCAAATA                                                                         >  SRR4302544.2979569/1‑94 (MQ=60)
                      CCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATA                                                                         >  SRR4302544.2979568/1‑94 (MQ=60)
                         CTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCC                                                                      >  SRR4302544.4024834/1‑94 (MQ=60)
                         CTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCC                                                                      <  SRR4302544.4754984/94‑1 (MQ=60)
                          TAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCC                                                                     <  SRR4302544.1511539/94‑1 (MQ=60)
                           AAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTCCGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCT                                                                    >  SRR4302544.1468877/1‑94 (MQ=60)
                           AAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCT                                                                    >  SRR4302544.4323014/1‑94 (MQ=60)
                           AAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCT                                                                    <  SRR4302544.4167873/94‑1 (MQ=60)
                           AAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCT                                                                    >  SRR4302544.1468878/1‑94 (MQ=60)
                           AAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCT                                                                    >  SRR4302544.1367720/1‑94 (MQ=60)
                            AACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCG‑AAATATCCCTGG                                                                  <  SRR4302544.2127137/94‑1 (MQ=60)
                             ACGATACCCAAAGGATTCTGGCCCTCGCGGAAAATATGCTCGACCCCTACGGCATCATCAGTCGCCAGGCGCTCATAGCCGAAAATATCCCTGG                                                                  <  SRR4302544.3668414/94‑1 (MQ=60)
                              CGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGC                                                                 >  SRR4302544.3579684/1‑94 (MQ=60)
                              CGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGC                                                                 >  SRR4302544.1498842/1‑94 (MQ=60)
                              CGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGC                                                                 <  SRR4302544.4451595/94‑1 (MQ=60)
                                ATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCAAAAATATCCCTGGCGG                                                               <  SRR4302544.1259661/94‑1 (MQ=60)
                                    CGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTT                                                           >  SRR4302544.1114050/1‑94 (MQ=60)
                                     GAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTC                                                          >  SRR4302544.449485/1‑94 (MQ=60)
                                      AAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCC                                                         >  SRR4302544.4245231/1‑94 (MQ=60)
                                         GGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATC                                                      >  SRR4302544.4723931/1‑94 (MQ=60)
                                             GCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATG                                                  <  SRR4302544.3730304/94‑1 (MQ=60)
                                                  CGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAAC                                             <  SRR4302544.3504420/94‑1 (MQ=60)
                                                  CGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAAC                                             <  SRR4302544.3638764/94‑1 (MQ=60)
                                                  CGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAAC                                             <  SRR4302544.1421694/94‑1 (MQ=60)
                                                   GCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACG                                            >  SRR4302544.4067264/1‑94 (MQ=60)
                                                       GCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTT                                        <  SRR4302544.4787362/94‑1 (MQ=60)
                                                        CGGAAAATATGCTCGACTGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTG                                       <  SRR4302544.4787056/94‑1 (MQ=60)
                                                         GGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAGTATCCCTGTCGGGGTTCCATCGACGCAAACGCTTTGT                                      >  SRR4302544.2819751/1‑94 (MQ=60)
                                                         GGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGT                                      >  SRR4302544.2819750/1‑94 (MQ=60)
                                                         GGAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGT                                      >  SRR4302544.1754686/1‑94 (MQ=60)
                                                          GAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTC                                     <  SRR4302544.3602299/94‑1 (MQ=60)
                                                          GAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTC                                     >  SRR4302544.3973772/1‑94 (MQ=60)
                                                          GAAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTC                                     <  SRR4302544.2304117/94‑1 (MQ=60)
                                                           AAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCG                                    <  SRR4302544.2892015/94‑1 (MQ=60)
                                                           AAAATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCG                                    >  SRR4302544.725686/1‑94 (MQ=60)
                                                              ATATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAG                                 <  SRR4302544.375649/94‑1 (MQ=60)
                                                                ATGCTCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTA                               >  SRR4302544.2087025/1‑94 (MQ=60)
                                                                    TCGACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGA                           >  SRR4302544.2305332/1‑94 (MQ=60)
                                                                      GACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGTGGGTTTCCATCGATGCAAACGTTTTGTCGAAGTATGGAAA                         >  SRR4302544.4380589/1‑94 (MQ=60)
                                                                      GACCGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAG                         <  SRR4302544.2659175/94‑1 (MQ=60)
                                                                         CGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACT                      <  SRR4302544.217312/94‑1 (MQ=60)
                                                                         CGCTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACT                      <  SRR4302544.1613832/94‑1 (MQ=60)
                                                                           CTACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCC                    >  SRR4302544.752238/1‑94 (MQ=60)
                                                                            TACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCG                   <  SRR4302544.334021/94‑1 (MQ=60)
                                                                            TACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCG                   >  SRR4302544.3029554/1‑94 (MQ=60)
                                                                             ACGGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGG                  <  SRR4302544.4554998/94‑1 (MQ=60)
                                                                               GGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGC                >  SRR4302544.4493921/1‑94 (MQ=60)
                                                                               GGCATCATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGC                >  SRR4302544.1193198/1‑94 (MQ=60)
                                                                                     ATCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTA          >  SRR4302544.3135279/1‑94 (MQ=60)
                                                                                      TCAGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTAT         <  SRR4302544.1458126/94‑1 (MQ=60)
                                                                                        AGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGC       <  SRR4302544.2842840/94‑1 (MQ=60)
                                                                                        AGTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGC       <  SRR4302544.550778/94‑1 (MQ=60)
                                                                                         GTCGCCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCG      >  SRR4302544.232976/1‑94 (MQ=60)
                                                                                             CCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCGAGGT  <  SRR4302544.1007355/94‑1 (MQ=60)
                                                                                             CCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCGAGGT  <  SRR4302544.1007354/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTGGTCGTTATTGCAGGTGGAGCCACTAAACGATACCGAAAGGATGCTGGCGCTGGCGGAAAATATGCTCGACCGCTACGGCATCATCAGTCGTCAGGCGGTGATAGCCGAAAATATCCCTGGCGGGTTTCCATCGATGCAAACGCTTTGTCGAAGTATGGAAGACTCCGGGCGAATTATGCGAGGT  >  NC_000913/1731082‑1731268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑