Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,749,776 T→C A693A (GCA→GCG)  b1673 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,749,7760TC99.2% 418.9 / ‑4.5 132A693A (GCA→GCG) b1673hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (60/71);  total (60/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-01

Only 100 of 136 total aligned reads displayed.

CGCCTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCTGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTCTTT  >  NC_000913/1749686‑1749865
                                                                                          |                                                                                         
cGCCTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGtgccgc                                                                                         >  2:2662356/1‑93 (MQ=255)
   cTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAg                                                                                      >  2:3064083/1‑93 (MQ=255)
   cTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAg                                                                                      >  1:625602/1‑93 (MQ=255)
    tCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCCGa                                                                                     >  2:4663819/1‑93 (MQ=255)
    tCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGa                                                                                     <  1:789625/93‑1 (MQ=255)
    tCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGa                                                                                     >  2:1162616/1‑93 (MQ=255)
    tCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGa                                                                                     >  2:167184/1‑93 (MQ=255)
    tCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGa                                                                                     >  2:453516/1‑93 (MQ=255)
      gTCAACTTCGCTGCCTTTCGCTAACGTCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATc                                                                                   <  1:4429716/93‑1 (MQ=255)
      gTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATc                                                                                   <  2:410937/93‑1 (MQ=255)
       tCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATcg                                                                                  <  1:4146333/93‑1 (MQ=255)
       tCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATcg                                                                                  <  1:4146334/93‑1 (MQ=255)
       tCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATcg                                                                                  >  1:4629125/1‑93 (MQ=255)
        cAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATcgg                                                                                 <  2:3782953/93‑1 (MQ=255)
         aaCTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATcggc                                                                                <  1:3570822/93‑1 (MQ=255)
           cTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATcggcgg                                                                              >  1:2818662/1‑93 (MQ=255)
            ttCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCggcggc                                                                             >  1:3696850/1‑93 (MQ=255)
             tCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCa                                                                            >  1:1740191/1‑93 (MQ=255)
              cgcgGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCaa                                                                           <  1:1038740/93‑1 (MQ=255)
              cGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCaa                                                                           <  2:775499/93‑1 (MQ=255)
               gCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAat                                                                          >  1:4388578/1‑93 (MQ=255)
                 tGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAatat                                                                        >  1:1573936/1‑93 (MQ=255)
                 tGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAatat                                                                        >  2:8632/1‑93 (MQ=255)
                  gACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATc                                                                       <  2:4540353/93‑1 (MQ=255)
                   aCTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCt                                                                      <  2:96571/93‑1 (MQ=255)
                   aCTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCt                                                                      <  2:96573/93‑1 (MQ=255)
                     tttCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTc                                                                    <  2:2783750/93‑1 (MQ=255)
                     tttCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTc                                                                    >  1:1053156/1‑93 (MQ=255)
                      ttCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTcc                                                                   <  2:2718364/93‑1 (MQ=255)
                      ttCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTcc                                                                   <  1:4016950/93‑1 (MQ=255)
                      ttCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTcc                                                                   <  1:3270018/93‑1 (MQ=255)
                      ttCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTcc                                                                   <  1:4519348/93‑1 (MQ=255)
                       tCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCa                                                                  <  1:3670158/93‑1 (MQ=255)
                       tCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCa                                                                  >  1:2928440/1‑93 (MQ=255)
                        cGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAg                                                                 <  1:2309531/93‑1 (MQ=255)
                        cGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAg                                                                 >  1:684806/1‑93 (MQ=255)
                         gttAACGGCAATTCATCCTGATGGTTCATCTTACGCATTCCTTACGCAGGCAGTAGATTGTGTGCCTCGAGATCGGCGGCAATATCTTCCAGc                                                                <  2:1800579/91‑1 (MQ=255)
                           tAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGccc                                                              >  1:2927476/1‑93 (MQ=255)
                            aaCGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGcccc                                                             <  1:2021547/93‑1 (MQ=255)
                             aCGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGccccc                                                            >  1:1327151/1‑92 (MQ=255)
                              cGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAg                                                           <  1:1384437/93‑1 (MQ=255)
                                gCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCTGCCAGATCGGCGGCAATATCTTCCAGCCCCAGAc                                                         <  2:1587986/93‑1 (MQ=255)
                                  cattcattcGGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGc                                                       <  1:1583164/92‑1 (MQ=255)
                                  aaTTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGc                                                       <  1:1583163/93‑1 (MQ=255)
                                  aaTTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAAATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGc                                                       >  1:4290969/1‑93 (MQ=255)
                                    ttCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTg                                                     <  1:3736093/93‑1 (MQ=255)
                                    ttCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTg                                                     <  2:4156915/93‑1 (MQ=255)
                                     tCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGc                                                    <  1:2725618/93‑1 (MQ=255)
                                     tCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGc                                                    >  1:1823491/1‑93 (MQ=255)
                                     tCATCCTGATGGTTCATCTTACCCATTCCTCACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGc                                                    <  2:3920171/93‑1 (MQ=255)
                                      cATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCa                                                   >  1:2228868/1‑93 (MQ=255)
                                       aTCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCaa                                                  >  1:4407453/1‑93 (MQ=255)
                                       aTCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCaa                                                  <  2:2014760/93‑1 (MQ=255)
                                        tCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGGTGCAAt                                                 >  2:3259899/1‑93 (MQ=255)
                                          cTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGt                                               <  1:1318382/93‑1 (MQ=255)
                                          cTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGt                                               >  2:3988586/1‑93 (MQ=255)
                                          cTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGt                                               <  2:3559390/93‑1 (MQ=255)
                                          cTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCAAGATCGGCGGCAATAAATTCCAGCCCCAGACGCTGCCATGt                                               >  2:860380/1‑93 (MQ=255)
                                           tGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATggt                                              >  2:2640669/1‑91 (MQ=255)
                                           tGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTACAGCCTCAAAAGCTGCAATCtt                                              >  1:1930196/1‑93 (MQ=255)
                                            gATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCTCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGttt                                             <  2:3340292/93‑1 (MQ=255)
                                            gATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGttt                                             >  2:135856/1‑93 (MQ=255)
                                            gATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGttt                                             >  2:135855/1‑93 (MQ=255)
                                             aTGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTc                                            <  2:295423/93‑1 (MQ=255)
                                             aTGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTc                                            <  2:4620362/93‑1 (MQ=255)
                                              tGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTcg                                           <  1:2242183/93‑1 (MQ=255)
                                               ggTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTcgc                                          <  1:3843816/93‑1 (MQ=255)
                                               ggTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTcgc                                          <  2:2069127/93‑1 (MQ=255)
                                                  tCATCTTACCCATGCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCggg                                       <  1:98270/93‑1 (MQ=255)
                                                   cATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGt                                      <  2:2817/93‑1 (MQ=255)
                                                     tCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTg                                    >  2:3487508/1‑93 (MQ=255)
                                                      cTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTgg                                   <  2:4522568/93‑1 (MQ=255)
                                                       ttccccATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGa                                  <  1:2851706/90‑1 (MQ=255)
                                                       ttACCCATTCCTTACGCAGGCAGTAGATTGTGTCCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGa                                  <  1:2449529/93‑1 (MQ=255)
                                                         aCCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGAGGGTTGGACa                                >  1:1127417/1‑93 (MQ=255)
                                                           ccATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAAc                              >  1:3915165/1‑93 (MQ=255)
                                                            cATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAAcc                             <  1:1845066/93‑1 (MQ=255)
                                                             aTTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCa                            >  2:4378415/1‑93 (MQ=255)
                                                               tCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAg                          >  2:3161055/1‑93 (MQ=255)
                                                                   tACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCtgt                      <  2:1159652/93‑1 (MQ=255)
                                                                    aCGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCtgtg                     <  2:3449752/93‑1 (MQ=255)
                                                                     cGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTgg                    <  2:4062801/93‑1 (MQ=255)
                                                                     cGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTgg                    <  2:43174/93‑1 (MQ=255)
                                                                      gcagccagTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTggg                   <  2:1631809/93‑1 (MQ=255)
                                                                       caggcagTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGt                  <  1:2083833/93‑1 (MQ=255)
                                                                        aggcagTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAATCTGTGGGTc                 <  1:955448/93‑1 (MQ=255)
                                                                         ggcagTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTcc                >  1:516414/1‑93 (MQ=255)
                                                                         ggcagGAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTcc                <  2:513955/93‑1 (MQ=255)
                                                                          gcagTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTccc               <  2:1498411/93‑1 (MQ=255)
                                                                           cagTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCa              <  1:3545396/93‑1 (MQ=255)
                                                                             gTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGGCGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGc            <  2:2006341/93‑1 (MQ=255)
                                                                              tAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGcc           >  2:2356789/1‑93 (MQ=255)
                                                                              tAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGcc           <  1:519003/93‑1 (MQ=255)
                                                                              tAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGcc           >  1:3891285/1‑93 (MQ=255)
                                                                               aGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGccc          >  1:2464471/1‑93 (MQ=255)
                                                                                    gtgtgCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTc     >  2:2060696/1‑93 (MQ=255)
                                                                                    gtgtgCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTc     <  2:1772859/93‑1 (MQ=255)
                                                                                     tgtgCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTCt    <  2:1660675/93‑1 (MQ=255)
                                                                                       tgccgcCAGATCGGCTGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTCttt  >  2:1185369/1‑93 (MQ=255)
                                                                                       tgccgcCAGATCGGCGGCAATATCTTCCAGCCCCTGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTCttt  <  2:2142199/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGCCTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCTGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTCTTT  >  NC_000913/1749686‑1749865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCGCCTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCTGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTCTTTGTAG  >  NC_000913/1749684‑1749869
                                                                                            |                                                                                             
TTCGCCTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCG                                                                                              >  SRR4302544.1750236/1‑94 (MQ=60)
    CCTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAG                                                                                          >  SRR4302544.1225655/1‑94 (MQ=60)
    CCTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAG                                                                                          >  SRR4302544.631751/1‑94 (MQ=60)
      TCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGAT                                                                                        <  SRR4302544.797260/94‑1 (MQ=60)
        GTCAACTTCGCTGCCTTTCGCTAACGTCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCG                                                                                      <  SRR4302544.4474414/94‑1 (MQ=60)
        GTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCG                                                                                      >  SRR4302544.4675018/1‑94 (MQ=60)
         TCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGG                                                                                     <  SRR4302544.4187383/94‑1 (MQ=60)
         TCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGG                                                                                     <  SRR4302544.4187384/94‑1 (MQ=60)
          CAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGC                                                                                    >  SRR4302544.311209/1‑94 (MQ=60)
           AACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCG                                                                                   <  SRR4302544.3605061/94‑1 (MQ=60)
            ACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGG                                                                                  >  SRR4302544.2845878/1‑94 (MQ=60)
            ACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGG                                                                                  <  SRR4302544.1868702/94‑1 (MQ=60)
             CTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGC                                                                                 >  SRR4302544.3733667/1‑94 (MQ=60)
              TTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCA                                                                                >  SRR4302544.1757229/1‑94 (MQ=60)
                CGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAAT                                                                              >  SRR4302544.4433001/1‑94 (MQ=60)
                CGCGGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAAT                                                                              <  SRR4302544.1049062/94‑1 (MQ=60)
                 GCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATA                                                                             >  SRR4302544.3686444/1‑94 (MQ=60)
                  CTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATAT                                                                            >  SRR4302544.1589495/1‑94 (MQ=60)
                      CTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTC                                                                        >  SRR4302544.1063598/1‑94 (MQ=60)
                        TTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCA                                                                      <  SRR4302544.4056906/94‑1 (MQ=60)
                        TTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCA                                                                      <  SRR4302544.4564602/94‑1 (MQ=60)
                        TTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCA                                                                      >  SRR4302544.2956580/1‑94 (MQ=60)
                        TTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCA                                                                      <  SRR4302544.3301212/94‑1 (MQ=60)
                         TCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAG                                                                     <  SRR4302544.1753893/94‑1 (MQ=60)
                         TCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAG                                                                     >  SRR4302544.691617/1‑94 (MQ=60)
                         TCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAG                                                                     <  SRR4302544.3706762/94‑1 (MQ=60)
                          CGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGC                                                                    <  SRR4302544.2331363/94‑1 (MQ=60)
                            CTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCC                                                                  >  SRR4302544.2955613/1‑94 (MQ=60)
                              AACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCC                                                                >  SRR4302544.1340203/1‑94 (MQ=60)
                              AACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCA                                                                <  SRR4302544.2041165/94‑1 (MQ=60)
                              AACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCA                                                                <  SRR4302544.2041164/94‑1 (MQ=60)
                              AACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCA                                                                >  SRR4302544.548735/1‑94 (MQ=60)
                                CGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGA                                                              <  SRR4302544.1398264/94‑1 (MQ=60)
                                   CAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAAATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGC                                                           >  SRR4302544.4334790/1‑94 (MQ=60)
                                    cattcattcgGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCT                                                          <  SRR4302544.1598804/84‑1 (MQ=60)
                                    AATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCT                                                          <  SRR4302544.1598803/94‑1 (MQ=60)
                                     ATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTG                                                         <  SRR4302544.2316139/94‑1 (MQ=60)
                                      TTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGC                                                        >  SRR4302544.1841258/1‑94 (MQ=60)
                                      TTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGC                                                        <  SRR4302544.3773232/94‑1 (MQ=60)
                                       TCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCA                                                       <  SRR4302544.2751865/94‑1 (MQ=60)
                                       TCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCA                                                       >  SRR4302544.2249839/1‑94 (MQ=60)
                                        CATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAA                                                      >  SRR4302544.4451968/1‑94 (MQ=60)
                                           CCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGT                                                   >  SRR4302544.3870748/1‑94 (MQ=60)
                                            CTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTT                                                  <  SRR4302544.1331344/94‑1 (MQ=60)
                                            CTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTACAGCCTCAAAAGCTGCAATCTT                                                  >  SRR4302544.1948873/1‑94 (MQ=60)
                                                TGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGC                                              <  SRR4302544.2263275/94‑1 (MQ=60)
                                                 GGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCG                                             <  SRR4302544.3881954/94‑1 (MQ=60)
                                                 GGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCG                                             >  SRR4302544.2703478/1‑94 (MQ=60)
                                                    TCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGT                                          <  SRR4302544.99171/94‑1 (MQ=60)
                                                    TCATCTTACCCATGCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGT                                          <  SRR4302544.99170/94‑1 (MQ=60)
                                                      ATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTG                                        >  SRR4302544.928957/1‑94 (MQ=60)
                                                         TTCCCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGAC                                     <  SRR4302544.2879258/94‑1 (MQ=60)
                                                         TTACCCATTCCTTACGCAGGCAGTAGATTGTGTCCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGAC                                     <  SRR4302544.2472893/94‑1 (MQ=60)
                                                          TACCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGAGGGTTGGACA                                    >  SRR4302544.1138655/1‑94 (MQ=60)
                                                            CCCATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAAC                                  >  SRR4302544.3954058/1‑94 (MQ=60)
                                                              CATTCCTTACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCA                                <  SRR4302544.1862941/94‑1 (MQ=60)
                                                                     TACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTG                         <  SRR4302544.4447471/94‑1 (MQ=60)
                                                                     TACGCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTG                         <  SRR4302544.3964978/94‑1 (MQ=60)
                                                                        GCAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGT                      >  SRR4302544.294539/1‑94 (MQ=60)
                                                                         CAGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTC                     <  SRR4302544.2103874/94‑1 (MQ=60)
                                                                          AGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAATCTGTGGGTCC                    <  SRR4302544.964838/94‑1 (MQ=60)
                                                                          AGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCC                    >  SRR4302544.521521/1‑94 (MQ=60)
                                                                          AGGCAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGGTGGACAACCAAGCTGTGGGTCC                    >  SRR4302544.4246532/1‑94 (MQ=60)
                                                                             CAGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAG                 <  SRR4302544.3579342/94‑1 (MQ=60)
                                                                              AGTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGC                >  SRR4302544.4630141/1‑94 (MQ=60)
                                                                               GTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCC               >  SRR4302544.3929939/1‑94 (MQ=60)
                                                                               GTAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGAAGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCC               >  SRR4302544.1578321/1‑94 (MQ=60)
                                                                                TAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCC              >  SRR4302544.2488004/1‑94 (MQ=60)
                                                                                TAGATTGTGTGCCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCC              <  SRR4302544.524127/94‑1 (MQ=60)
                                                                                           CCGCCAGATCGGCGGCTATCTCTTCCAGCCCCAGACGCTGCCATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTCTTTGTA   <  SRR4302544.4237754/94‑1 (MQ=60)
                                                                                           CCGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTCTTTGTA   <  SRR4302544.2337901/94‑1 (MQ=60)
                                                                                            CGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTCTTTGTAG  <  SRR4302544.3411206/94‑1 (MQ=60)
                                                                                            |                                                                                             
TTCGCCTCGTCAACTTCGCTGACTTTCGCTAACGGCAATTCATCCTGATGGTTCATCTTACCCATTCCTTACGCAGGCAGTAGATTGTGTGCTGCCAGATCGGCGGCAATATCTTCCAGCCCCAGACGCTGCAATGTTTCGCGGGTTGGACAACCAAGCTGTGGGTCCCAGCCCATTTCTTTGTAG  >  NC_000913/1749684‑1749869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑