Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,750,491 T→C Y455C (TAC→TGC)  b1673 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,750,4910TC98.6% 433.6 / ‑3.7 139Y455C (TAC→TGC) b1673hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (69/67);  total (71/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 149 total aligned reads displayed.

ACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAG  >  NC_000913/1750401‑1750580
                                                                                          |                                                                                         
 cAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCAct                                                                                        <  2:1087091/93‑1 (MQ=255)
  aaTGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCActc                                                                                       >  1:3899941/1‑93 (MQ=255)
   aTGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCActct                                                                                      >  1:1037326/1‑93 (MQ=255)
    tGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCtt                                                                                     >  2:1720097/1‑93 (MQ=255)
    tGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCtt                                                                                     >  2:104923/1‑93 (MQ=255)
       aaCCGAACTGAGCTGCCGCTTCATTGGCATGGTGAACAGGATAGCGAAACGGCGACCAGAGTTTATTTTTCGAGTAGCGCCAGCACTCTTCAc                                                                                  >  2:2832858/1‑93 (MQ=255)
         ccGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCAccc                                                                                >  1:4389269/1‑93 (MQ=255)
          cGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCa                                                                               <  2:450161/93‑1 (MQ=255)
           gACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCaa                                                                              >  1:2756600/1‑93 (MQ=255)
            aCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCaaa                                                                             >  1:3616686/1‑93 (MQ=255)
            aCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCaaa                                                                             >  1:2272493/1‑93 (MQ=255)
              cTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAtt                                                                           >  1:3380178/1‑93 (MQ=255)
               tGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTc                                                                          >  1:234848/1‑93 (MQ=255)
               tGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTc                                                                          >  1:555742/1‑93 (MQ=255)
               tGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTc                                                                          <  1:999298/93‑1 (MQ=255)
                 cgcTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCa                                                                        <  2:779908/93‑1 (MQ=255)
                  gcTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAg                                                                       <  1:4350794/93‑1 (MQ=255)
                   cTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGc                                                                      >  2:533197/1‑93 (MQ=255)
                     gACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGt                                                                    <  1:4301662/93‑1 (MQ=255)
                     gACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGt                                                                    >  1:1364102/1‑93 (MQ=255)
                     gACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGt                                                                    <  1:2812232/93‑1 (MQ=255)
                       cGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTc                                                                  <  1:1093668/93‑1 (MQ=255)
                        gCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCt                                                                 <  2:2087632/93‑1 (MQ=255)
                        gCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCt                                                                 >  1:3305433/1‑93 (MQ=255)
                        gCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCt                                                                 >  2:1454458/1‑93 (MQ=255)
                        gCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCt                                                                 <  1:202976/93‑1 (MQ=255)
                         cTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTg                                                                >  2:905290/1‑93 (MQ=255)
                          ttCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGc                                                               <  2:4627687/93‑1 (MQ=255)
                          ttCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGc                                                               <  1:1614113/93‑1 (MQ=255)
                              ttGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATg                                                           <  2:1497277/93‑1 (MQ=255)
                                ggCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGc                                                         >  1:1622914/1‑93 (MQ=255)
                                  cATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCat                                                       >  2:3062701/1‑93 (MQ=255)
                                   aTGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCata                                                      >  2:3923574/1‑93 (MQ=255)
                                    tGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCatat                                                     <  1:918755/93‑1 (MQ=255)
                                         aaCCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAcc                                                >  1:4538459/1‑93 (MQ=255)
                                         aaCCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAcc                                                <  2:3511843/93‑1 (MQ=255)
                                          aCCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCa                                               >  1:3090696/1‑93 (MQ=255)
                                          aCCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCa                                               <  1:3672271/93‑1 (MQ=255)
                                          aCCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCa                                               <  1:3672394/93‑1 (MQ=255)
                                          aCCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCa                                               <  2:3380343/93‑1 (MQ=255)
                                          aCCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGAACTCTTCACCCAAATTCTAGCGTTCTGCGATGGCATATGAAcaa                                               >  2:2490946/1‑91 (MQ=255)
                                            cGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCACATTCCAGCGTTCTGCGATGGCATATGAACCATc                                             >  1:4577663/1‑93 (MQ=255)
                                            cGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATc                                             <  2:4372090/93‑1 (MQ=255)
                                            cGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATc                                             <  2:1454011/93‑1 (MQ=255)
                                            cGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATc                                             <  1:2273813/93‑1 (MQ=255)
                                             ggATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCa                                            >  2:1580015/1‑93 (MQ=255)
                                             ggATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCa                                            >  2:1343628/1‑93 (MQ=255)
                                             ggATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCa                                            <  1:3769037/93‑1 (MQ=255)
                                             ggATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCa                                            <  2:3581391/93‑1 (MQ=255)
                                             ggATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCa                                            <  1:845098/93‑1 (MQ=255)
                                              gATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAg                                           <  2:2675326/93‑1 (MQ=255)
                                              gATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAg                                           <  2:1373947/93‑1 (MQ=255)
                                                tAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGcc                                         <  2:2797186/93‑1 (MQ=255)
                                                tAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGcc                                         >  2:2025525/1‑93 (MQ=255)
                                                  gCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGGACTCTTCACTCAAATTCCGGCGTTCGGCGCTGGCATATGAACCATCAGCGAg                                       >  1:2711725/1‑93 (MQ=255)
                                                   ccAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAgg                                      >  1:917898/1‑93 (MQ=255)
                                                    cAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGt                                     <  1:360128/93‑1 (MQ=255)
                                                     aaaCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTg                                    >  1:996912/1‑93 (MQ=255)
                                                     aaaCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTg                                    >  1:4427253/1‑93 (MQ=255)
                                                      accGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGa                                   <  1:2572264/91‑1 (MQ=255)
                                                      aaCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGa                                   >  2:1966937/1‑93 (MQ=255)
                                                      aaCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGa                                   <  1:2572263/93‑1 (MQ=255)
                                                        cGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACt                                 >  2:2909897/1‑93 (MQ=255)
                                                         ggCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTc                                <  2:2213240/93‑1 (MQ=255)
                                                          gCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCa                               <  1:2632741/93‑1 (MQ=255)
                                                              ccAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTc                           >  1:481027/1‑93 (MQ=255)
                                                               cAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCa                          >  2:1148298/1‑93 (MQ=255)
                                                                agagTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAc                         >  2:557447/1‑93 (MQ=255)
                                                                agagTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAc                         >  2:937736/1‑93 (MQ=255)
                                                                 gagTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAcc                        >  2:2473031/1‑93 (MQ=255)
                                                                    tttATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCcac                     >  2:2270299/1‑93 (MQ=255)
                                                                    tttATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCcac                     <  2:1244617/93‑1 (MQ=255)
                                                                      tATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCacac                   <  2:3684436/93‑1 (MQ=255)
                                                                       aTTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACg                  >  2:3277551/1‑93 (MQ=255)
                                                                        tttttCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGa                 <  2:4583711/93‑1 (MQ=255)
                                                                         ttttCGCGTAGCCCCAGCCCTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGAt                <  2:1025270/93‑1 (MQ=255)
                                                                         ttttCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGAt                <  2:604358/93‑1 (MQ=255)
                                                                              gcgTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGcc           >  1:370457/1‑93 (MQ=255)
                                                                              gcgTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGcc           >  2:186314/1‑93 (MQ=255)
                                                                              gcgTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGcc           >  1:2338599/1‑93 (MQ=255)
                                                                               cgTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCa          <  2:2310796/93‑1 (MQ=255)
                                                                               cgTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCa          >  2:2662378/1‑93 (MQ=255)
                                                                                gTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAg         >  1:650668/1‑93 (MQ=255)
                                                                                gTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAg         <  1:2005207/93‑1 (MQ=255)
                                                                                 tAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGa        <  1:3066230/93‑1 (MQ=255)
                                                                                  aGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAc       >  2:2334643/1‑93 (MQ=255)
                                                                                   gCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACg      <  1:929412/93‑1 (MQ=255)
                                                                                   gCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACg      <  1:1129038/93‑1 (MQ=255)
                                                                                    ccccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATAAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACgg     <  1:1542645/93‑1 (MQ=255)
                                                                                     cgcAGCACTCTTCACCCAAATCCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACGCGATGCGCCAGACGgt    <  2:325042/91‑1 (MQ=255)
                                                                                     cccAGCACTCTTCCCCCAAATTCCAGCGTTCTGCGATGGCATATGAGCCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgt    <  2:383281/93‑1 (MQ=255)
                                                                                     cccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgt    <  2:1787630/93‑1 (MQ=255)
                                                                                     cccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgt    >  1:2592422/1‑93 (MQ=255)
                                                                                      ccAGCACTCTTCGCCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgta   <  2:1651558/93‑1 (MQ=255)
                                                                                      ccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgtg   >  1:2870038/1‑92 (MQ=255)
                                                                                      ccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgta   >  2:3583792/1‑93 (MQ=255)
                                                                                      ccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgta   <  1:716469/93‑1 (MQ=255)
                                                                                      ccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgta   >  1:3859374/1‑93 (MQ=255)
                                                                                      ccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgta   >  2:1983597/1‑93 (MQ=255)
                                                                                       cAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgtag  >  2:340231/1‑93 (MQ=255)
                                                                                          |                                                                                         
ACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAG  >  NC_000913/1750401‑1750580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAA  >  NC_000913/1750399‑1750583
                                                                                            |                                                                                            
TAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCA                                                                                             >  SRR4302544.994954/1‑94 (MQ=60)
 AACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCAC                                                                                            <  SRR4302544.3581485/94‑1 (MQ=60)
  ACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACT                                                                                           <  SRR4302544.2603994/94‑1 (MQ=60)
   CAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTC                                                                                          >  SRR4302544.3938678/1‑94 (MQ=60)
    AATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCACACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCT                                                                                         >  SRR4302544.999715/1‑94 (MQ=60)
    AATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCT                                                                                         <  SRR4302544.2351437/94‑1 (MQ=60)
    AATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCT                                                                                         >  SRR4302544.1047637/1‑94 (MQ=60)
      TGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTC                                                                                       >  SRR4302544.1573585/1‑94 (MQ=60)
       GGAACCGACCTGCCCGGACGCTTCATTGGCATGGTGAACCGGATAGGCCAACGGCGACCGGAGTGTATTTTTCGCGGAGCCCCAGCACTCTTCA                                                                                      <  SRR4302544.378913/94‑1 (MQ=60)
          ACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCC                                                                                   >  SRR4302544.4433694/1‑94 (MQ=60)
            CGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAA                                                                                 >  SRR4302544.2783134/1‑94 (MQ=60)
             GACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAA                                                                                >  SRR4302544.3652452/1‑94 (MQ=60)
             GACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAA                                                                                >  SRR4302544.2487261/1‑94 (MQ=60)
             GACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAA                                                                                >  SRR4302544.2293887/1‑94 (MQ=60)
               CCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATT                                                                              >  SRR4302544.4292901/1‑94 (MQ=60)
               CCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATT                                                                              >  SRR4302544.3412449/1‑94 (MQ=60)
                CTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTC                                                                             >  SRR4302544.561181/1‑94 (MQ=60)
                CTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTC                                                                             >  SRR4302544.237289/1‑94 (MQ=60)
                 TGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCC                                                                            <  SRR4302544.1009190/94‑1 (MQ=60)
                 TGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCC                                                                            <  SRR4302544.1009191/94‑1 (MQ=60)
                   CGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAG                                                                          <  SRR4302544.632797/94‑1 (MQ=60)
                    GCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGC                                                                         <  SRR4302544.4394999/94‑1 (MQ=60)
                      TGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGT                                                                       >  SRR4302544.1377660/1‑94 (MQ=60)
                       GACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTT                                                                      <  SRR4302544.4345545/94‑1 (MQ=60)
                       GACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTT                                                                      <  SRR4302544.2839372/94‑1 (MQ=60)
                         CGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCT                                                                    >  SRR4302544.3336918/1‑94 (MQ=60)
                         CGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCT                                                                    <  SRR4302544.1104549/94‑1 (MQ=60)
                         CGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGCTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAACTTCCAGCGTTCT                                                                    >  SRR4302544.1910805/1‑94 (MQ=60)
                          GCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTG                                                                   <  SRR4302544.205041/94‑1 (MQ=60)
                          GCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTG                                                                   >  SRR4302544.1155082/1‑94 (MQ=60)
                            TTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCG                                                                 <  SRR4302544.1630025/94‑1 (MQ=60)
                             TCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGA                                                                >  SRR4302544.2819864/1‑94 (MQ=60)
                              CATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGAT                                                               <  SRR4302544.2071677/94‑1 (MQ=60)
                                 TGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGC                                                            >  SRR4302544.1638904/1‑94 (MQ=60)
                                      TGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATG                                                       <  SRR4302544.927824/94‑1 (MQ=60)
                                          GAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACC                                                   >  SRR4302544.4583833/1‑94 (MQ=60)
                                           AACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCA                                                  >  SRR4302544.3120142/1‑94 (MQ=60)
                                            ACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCAT                                                 <  SRR4302544.3709023/94‑1 (MQ=60)
                                            ACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCAT                                                 <  SRR4302544.3708898/94‑1 (MQ=60)
                                             CCGGATAGCCAAACGGCGGCCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCACATTCCCGCGTTCTGCGATGGCATATGAACCATC                                                >  SRR4302544.2659323/1‑94 (MQ=60)
                                             CCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCACATTCCAGCGTTCTGCGATGGCATATGAACCATC                                                >  SRR4302544.4623268/1‑94 (MQ=60)
                                              CGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCA                                               <  SRR4302544.2295221/94‑1 (MQ=60)
                                               GGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAG                                              <  SRR4302544.853441/94‑1 (MQ=60)
                                               GGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAG                                              <  SRR4302544.3806410/94‑1 (MQ=60)
                                               GGATAGCAAAACGGGGTGCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCAAATTTCCAGCGTTCTGCGATGGCATATGAACCATCAG                                              <  SRR4302544.853440/94‑1 (MQ=60)
                                                GATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGC                                             <  SRR4302544.2952019/94‑1 (MQ=60)
                                                  TAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCA                                           >  SRR4302544.927367/1‑94 (MQ=60)
                                                   AGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGGACTCTTCACTCAAATTCCGGCGTTCGGCGCTGGCATATGAACCATCAGCGAG                                          >  SRR4302544.2737806/1‑94 (MQ=60)
                                                    GCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGG                                         >  SRR4302544.926959/1‑94 (MQ=60)
                                                      CAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTG                                       <  SRR4302544.363814/94‑1 (MQ=60)
                                                      CAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTG                                       >  SRR4302544.4471925/1‑94 (MQ=60)
                                                      CAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTG                                       >  SRR4302544.1006775/1‑94 (MQ=60)
                                                        ACCGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGAC                                     <  SRR4302544.2596978/94‑1 (MQ=60)
                                                        AACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGAC                                     <  SRR4302544.2596977/94‑1 (MQ=60)
                                                        AACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGAC                                     <  SRR4302544.3497413/94‑1 (MQ=60)
                                                         ACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACT                                    <  SRR4302544.3810719/94‑1 (MQ=60)
                                                            GCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAG                                 <  SRR4302544.2657977/94‑1 (MQ=60)
                                                            GCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAG                                 <  SRR4302544.4792636/94‑1 (MQ=60)
                                                            GCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAG                                 >  SRR4302544.3145093/1‑94 (MQ=60)
                                                               ACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTC                              >  SRR4302544.485735/1‑94 (MQ=60)
                                                                            TTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGC                 >  SRR4302544.3656254/1‑94 (MQ=60)
                                                                               CGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCC              >  SRR4302544.2360757/1‑94 (MQ=60)
                                                                               CGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCC              >  SRR4302544.374239/1‑94 (MQ=60)
                                                                                 CGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAG            >  SRR4302544.657104/1‑94 (MQ=60)
                                                                                  GTAGCCCCAGCACTCTTCGCCCAAATTCCAGCGTTCTGCGATGGCAGATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGA           <  SRR4302544.4420814/94‑1 (MQ=60)
                                                                                  GTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGA           <  SRR4302544.2024688/94‑1 (MQ=60)
                                                                                   TAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAC          <  SRR4302544.3095462/94‑1 (MQ=60)
                                                                                     GCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGG        <  SRR4302544.4566697/94‑1 (MQ=60)
                                                                                     GCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGG        <  SRR4302544.1140294/94‑1 (MQ=60)
                                                                                     GCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGG        <  SRR4302544.938570/94‑1 (MQ=60)
                                                                                      CCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGT       >  SRR4302544.2617363/1‑94 (MQ=60)
                                                                                      CCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATAAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGT       <  SRR4302544.1557991/94‑1 (MQ=60)
                                                                                       CCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTG      >  SRR4302544.2897740/1‑94 (MQ=60)
                                                                                       CCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTA      >  SRR4302544.3897690/1‑94 (MQ=60)
                                                                                       CCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTA      >  SRR4302544.373780/1‑94 (MQ=60)
                                                                                        CCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAG     <  SRR4302544.723547/94‑1 (MQ=60)
                                                                                        CCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAG     <  SRR4302544.2662493/94‑1 (MQ=60)
                                                                                           GCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAA  <  SRR4302544.1754250/94‑1 (MQ=60)
                                                                                            |                                                                                            
TAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAA  >  NC_000913/1750399‑1750583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑