Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,750,491 T→C Y455C (TAC→TGC)  b1673 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,750,4910TC99.6% 725.0 / ‑4.8 224Y455C (TAC→TGC) b1673hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (117/106);  total (117/107)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

Only 100 of 229 total aligned reads displayed.

ACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAG  >  NC_000913/1750401‑1750580
                                                                                           |                                                                                         
aCAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCAc                                                                                         <  2:4399540/93‑1 (MQ=255)
  aaTGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCActc                                                                                       >  1:3948833/1‑93 (MQ=255)
  aaTGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCActc                                                                                       >  2:2167181/1‑93 (MQ=255)
  aaTGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCActc                                                                                       <  2:315300/93‑1 (MQ=255)
  aaTGGAACCGACCTGCGCCGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCActc                                                                                       <  1:2748002/93‑1 (MQ=255)
   aTGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCActct                                                                                      >  2:5418198/1‑93 (MQ=255)
   aTGGAACCGACCTGCGCTGACCCTTAATTGGCAAGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCActct                                                                                      <  2:4429408/93‑1 (MQ=255)
     ggAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTc                                                                                    <  2:630870/93‑1 (MQ=255)
     ggAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTc                                                                                    <  2:3343526/93‑1 (MQ=255)
      gAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCa                                                                                   >  2:6657897/1‑93 (MQ=255)
       aaCCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑ATTGTTTTTCGCGTAGCCCCAGCACTCTTCAc                                                                                  >  1:4599667/1‑93 (MQ=255)
        aCCGACCTGCGCCGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCAcc                                                                                 >  1:6587761/1‑93 (MQ=255)
          cGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCa                                                                               >  1:1802565/1‑93 (MQ=255)
            aCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCGCTCTTCACCCaaa                                                                             >  2:5873578/1‑93 (MQ=255)
            aCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCaaa                                                                             >  2:3437197/1‑93 (MQ=255)
            aCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCaaa                                                                             <  2:5259419/93‑1 (MQ=255)
            aCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCaaa                                                                             >  2:810790/1‑93 (MQ=255)
            aCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCaaa                                                                             >  1:1445947/1‑93 (MQ=255)
             ccTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAAt                                                                            <  2:1390481/93‑1 (MQ=255)
               tGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTc                                                                          >  1:5359820/1‑93 (MQ=255)
                   cTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGc                                                                      >  1:498798/1‑93 (MQ=255)
                   cTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGc                                                                      <  2:6468500/93‑1 (MQ=255)
                   cTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TGTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGc                                                                      <  2:3555864/93‑1 (MQ=255)
                    tGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCg                                                                     >  2:620049/1‑93 (MQ=255)
                       cGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTc                                                                  <  2:4294121/93‑1 (MQ=255)
                       cGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTc                                                                  <  2:4213743/93‑1 (MQ=255)
                       cGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTc                                                                  >  2:6631059/1‑93 (MQ=255)
                        gCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCt                                                                 <  1:4422345/93‑1 (MQ=255)
                        gCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCt                                                                 >  2:2294382/1‑93 (MQ=255)
                        gCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCt                                                                 >  1:307726/1‑93 (MQ=255)
                          ttCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTgg                                                               >  2:4958066/1‑92 (MQ=255)
                           tCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCg                                                              >  1:4121707/1‑93 (MQ=255)
                             aTTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGAt                                                            >  2:190323/1‑93 (MQ=255)
                              ttagCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATg                                                           >  2:2719552/4‑93 (MQ=255)
                              ttGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTCCTGCGATg                                                           <  1:1723393/93‑1 (MQ=255)
                              ttGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGGTCTGCGATg                                                           >  1:2931972/1‑93 (MQ=255)
                               tGGCATGGTGAATCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATgg                                                          <  2:5082108/93‑1 (MQ=255)
                               tGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATgg                                                          >  1:1027712/1‑93 (MQ=255)
                               tGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATgg                                                          <  1:5698497/93‑1 (MQ=255)
                                 gCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCa                                                        >  2:1514250/1‑93 (MQ=255)
                                 gCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCa                                                        <  2:4630209/93‑1 (MQ=255)
                                   aTGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGtata                                                      <  1:5550143/93‑1 (MQ=255)
                                   aTGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCata                                                      >  1:4971830/1‑93 (MQ=255)
                                     ggTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATg                                                    >  2:1581804/1‑93 (MQ=255)
                                     ggTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATg                                                    <  1:5114681/93‑1 (MQ=255)
                                      gTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGa                                                   <  1:2033376/93‑1 (MQ=255)
                                        gAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAc                                                 <  1:1164548/93‑1 (MQ=255)
                                         aaCCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAcc                                                >  2:6355773/1‑93 (MQ=255)
                                            cGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATc                                             <  2:319647/93‑1 (MQ=255)
                                            cGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATc                                             >  1:3815161/1‑93 (MQ=255)
                                             ggATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCa                                            <  2:1845324/93‑1 (MQ=255)
                                             ggATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCa                                            <  1:4164942/93‑1 (MQ=255)
                                              gATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAg                                           >  1:2403436/1‑93 (MQ=255)
                                               aTAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGc                                          <  2:2246867/93‑1 (MQ=255)
                                                tAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCCCTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGcc                                         <  1:3207147/93‑1 (MQ=255)
                                                tAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGcc                                         <  2:4686739/93‑1 (MQ=255)
                                                tAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGcc                                         <  1:3972949/93‑1 (MQ=255)
                                                 tagcAAACGGCCGCCCGAGTTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGcc                                         <  1:6153736/90‑1 (MQ=255)
                                                 aGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCa                                        <  1:3416989/93‑1 (MQ=255)
                                                 aGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCa                                        >  2:3892048/1‑93 (MQ=255)
                                                  gCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAg                                       <  1:240496/93‑1 (MQ=255)
                                                  gCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAg                                       >  1:6531632/1‑93 (MQ=255)
                                                   ccAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAgg                                      >  1:2160780/1‑93 (MQ=255)
                                                    cAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGt                                     <  1:2146158/93‑1 (MQ=255)
                                                     aaaCGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTg                                    >  2:5512960/1‑93 (MQ=255)
                                                     aaaCGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTg                                    >  2:4758731/1‑93 (MQ=255)
                                                       aCGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGAc                                  >  1:2248256/1‑93 (MQ=255)
                                                          gCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCa                               <  2:3672932/93‑1 (MQ=255)
                                                            gACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATTGCATATGAACCATCAGCCAGGTGACTCAGc                             >  2:2943671/1‑93 (MQ=255)
                                                            gACCAGAG‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGc                             >  2:4521330/1‑93 (MQ=255)
                                                                agag‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAc                         >  1:4759628/1‑93 (MQ=255)
                                                                 gag‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAcc                        >  1:5826812/1‑93 (MQ=255)
                                                                 gag‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAcc                        >  2:2384351/1‑93 (MQ=255)
                                                                   g‑TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCca                      >  2:5221944/1‑93 (MQ=255)
                                                                     tttATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCcac                     <  1:1093103/93‑1 (MQ=255)
                                                                      ttATTTTTCGCGTAGCCGCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCcaca                    <  1:1994607/93‑1 (MQ=255)
                                                                      ttATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCcaca                    >  1:3301936/1‑93 (MQ=255)
                                                                       tATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCacac                   >  1:1368123/1‑93 (MQ=255)
                                                                        aTTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACg                  >  1:5386978/1‑93 (MQ=255)
                                                                         tttttCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACgc                 >  1:1526931/1‑92 (MQ=255)
                                                                         tttttCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGa                 <  1:3539367/93‑1 (MQ=255)
                                                                         tttttCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGa                 <  1:230422/93‑1 (MQ=255)
                                                                          ttttCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGAt                <  1:6016837/93‑1 (MQ=255)
                                                                          ttttCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGAt                <  1:2841199/93‑1 (MQ=255)
                                                                               gcgTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGcc           <  1:6426085/93‑1 (MQ=255)
                                                                               gcgTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGcc           >  2:5025073/1‑93 (MQ=255)
                                                                               gcgTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGcc           >  2:695858/1‑93 (MQ=255)
                                                                                 gTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAg         >  1:1747296/1‑93 (MQ=255)
                                                                                 gTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAg         >  1:3900840/1‑93 (MQ=255)
                                                                                 gTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAg         >  1:6659046/1‑93 (MQ=255)
                                                                                   aGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAc       <  1:3772730/93‑1 (MQ=255)
                                                                                   aGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAc       <  1:4341980/93‑1 (MQ=255)
                                                                                   aGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAc       <  1:1889494/93‑1 (MQ=255)
                                                                                   aGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAc       >  1:6269963/1‑93 (MQ=255)
                                                                                   aGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAc       <  2:2060440/93‑1 (MQ=255)
                                                                                    gCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACg      <  2:6389201/93‑1 (MQ=255)
                                                                                     ccccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACgg     <  1:906860/93‑1 (MQ=255)
                                                                                      cccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACACAGCTCACCCACACGATGCGCCAGACGgt    <  2:3073618/93‑1 (MQ=255)
                                                                                       ccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgta   >  2:6026694/1‑93 (MQ=255)
                                                                                       ccAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGgta   <  2:3976133/93‑1 (MQ=255)
                                                                                           |                                                                                         
ACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAG‑TTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAG  >  NC_000913/1750401‑1750580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAAA  >  NC_000913/1750399‑1750584
                                                                                            |                                                                                             
TAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCA                                                                                              <  SRR4302536.6265640/94‑1 (MQ=60)
TAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCA                                                                                              >  SRR4302536.2579315/1‑94 (MQ=60)
TAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCA                                                                                              >  SRR4302536.5663374/1‑94 (MQ=60)
TAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGCTTATTTTTCGCGTAGCCCCAGCA                                                                                              >  SRR4302536.1208134/1‑94 (MQ=60)
 AACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCTGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCAC                                                                                             <  SRR4302536.6568530/94‑1 (MQ=60)
 AACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCAC                                                                                             >  SRR4302536.1778306/1‑94 (MQ=60)
  ACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACT                                                                                            >  SRR4302536.5123422/1‑94 (MQ=60)
   CAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTC                                                                                           >  SRR4302536.4066118/1‑94 (MQ=60)
    AATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCT                                                                                          <  SRR4302536.246866/94‑1 (MQ=60)
    AATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCT                                                                                          >  SRR4302536.117207/1‑94 (MQ=60)
    AATGGAACCGACCTGCGCCGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCT                                                                                          <  SRR4302536.2829829/94‑1 (MQ=60)
      TGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAACCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTC                                                                                        >  SRR4302536.1823524/1‑94 (MQ=60)
        GAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGATTGTTTTTCGCGTAGCCCCAGCACTCTTCAC                                                                                      >  SRR4302536.4737776/1‑94 (MQ=60)
         AACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACC                                                                                     >  SRR4302536.1416197/1‑94 (MQ=60)
         AACCGACCTGCGCCGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACC                                                                                     >  SRR4302536.6783741/1‑94 (MQ=60)
           CCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCA                                                                                   >  SRR4302536.1856978/1‑94 (MQ=60)
             GACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAA                                                                                 >  SRR4302536.1488797/1‑94 (MQ=60)
               CCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATT                                                                               <  SRR4302536.3955664/94‑1 (MQ=60)
                CTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTC                                                                              >  SRR4302536.5519730/1‑94 (MQ=60)
                CTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTC                                                                              >  SRR4302536.1788701/1‑94 (MQ=60)
                   CGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCCCCCAAATTCCAG                                                                           <  SRR4302536.6771377/94‑1 (MQ=60)
                   CGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAG                                                                           >  SRR4302536.5882838/1‑94 (MQ=60)
                    GCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCGGC                                                                          >  SRR4302536.5536298/1‑94 (MQ=60)
                    GCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGC                                                                          >  SRR4302536.511671/1‑94 (MQ=60)
                    GCTGACGCTTCATTGGCATGGAGAACCGGATAGCCAAACGGCGACCAGAGCTTATTTTTCGCGTAGCCTCAGCACTCTTCACCCAAATTCCAGC                                                                          >  SRR4302536.2016844/1‑94 (MQ=60)
                       GACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGAT                                                                       >  SRR4302536.3542995/1‑94 (MQ=60)
                         CGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCTAGCGTTCT                                                                     <  SRR4302536.6804056/94‑1 (MQ=60)
                         CGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCT                                                                     <  SRR4302536.2059116/94‑1 (MQ=60)
                         CGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCT                                                                     >  SRR4302536.315902/1‑94 (MQ=60)
                         CGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCT                                                                     <  SRR4302536.927032/94‑1 (MQ=60)
                          GCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTG                                                                    <  SRR4302536.4554789/94‑1 (MQ=60)
                           CTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGC                                                                   >  SRR4302536.3132293/1‑94 (MQ=60)
                            TTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCG                                                                  >  SRR4302536.51147/1‑94 (MQ=60)
                            TTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCG                                                                  >  SRR4302536.4243916/1‑94 (MQ=60)
                            TTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCG                                                                  <  SRR4302536.4240982/94‑1 (MQ=60)
                              CATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGAT                                                                <  SRR4302536.6444100/94‑1 (MQ=60)
                               ATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATG                                                               <  SRR4302536.210875/94‑1 (MQ=60)
                               ATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGGTCTGCGATG                                                               >  SRR4302536.3018485/1‑94 (MQ=60)
                                TTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGG                                                              >  SRR4302536.1058151/1‑94 (MQ=60)
                                TTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTCCTGCGATGG                                                              <  SRR4302536.1773171/94‑1 (MQ=60)
                                 TGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGC                                                             <  SRR4302536.5867938/94‑1 (MQ=60)
                                 TGGCATGGGGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGC                                                             <  SRR4302536.801034/94‑1 (MQ=60)
                                    CATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATA                                                          >  SRR4302536.3307583/1‑94 (MQ=60)
                                    CATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATA                                                          >  SRR4302536.5121167/1‑94 (MQ=60)
                                     ATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGTATAT                                                         <  SRR4302536.5715483/94‑1 (MQ=60)
                                       GGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGA                                                       <  SRR4302536.5267529/94‑1 (MQ=60)
                                        GTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAA                                                      <  SRR4302536.2094608/94‑1 (MQ=60)
                                         TGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAC                                                     <  SRR4302536.2768514/94‑1 (MQ=60)
                                         GGAACCCGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAC                                                     <  SRR4302536.3911407/94‑1 (MQ=60)
                                          GAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACC                                                    >  SRR4302536.6099250/1‑94 (MQ=60)
                                          GAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACC                                                    <  SRR4302536.1199602/94‑1 (MQ=60)
                                          GAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACC                                                    <  SRR4302536.2445533/94‑1 (MQ=60)
                                           AACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCA                                                   <  SRR4302536.4325369/94‑1 (MQ=60)
                                            ACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCAT                                                  >  SRR4302536.448290/1‑94 (MQ=60)
                                             CCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATC                                                 >  SRR4302536.3928858/1‑94 (MQ=60)
                                               GGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAG                                               >  SRR4302536.2476158/1‑94 (MQ=60)
                                               GGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAG                                               <  SRR4302536.4072838/94‑1 (MQ=60)
                                               GGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAG                                               <  SRR4302536.4288683/94‑1 (MQ=60)
                                                  tagcaaacggccgcccgagtTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCA                                            <  SRR4302536.6337069/74‑1 (MQ=60)
                                                  TAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCCCTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCA                                            <  SRR4302536.3301215/94‑1 (MQ=60)
                                                  TAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCA                                            <  SRR4302536.4090871/94‑1 (MQ=60)
                                                   AGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAG                                           >  SRR4302536.6726186/1‑94 (MQ=60)
                                                   AGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAG                                           <  SRR4302536.3518684/94‑1 (MQ=60)
                                                   AGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAG                                           >  SRR4302536.4878596/1‑94 (MQ=60)
                                                    GCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGG                                          <  SRR4302536.2283194/94‑1 (MQ=60)
                                                    GCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGG                                          >  SRR4302536.1880007/1‑94 (MQ=60)
                                                    GCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGG                                          >  SRR4302536.2225774/1‑94 (MQ=60)
                                                    GCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGG                                          <  SRR4302536.246896/94‑1 (MQ=60)
                                                     CCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGT                                         >  SRR4302536.4267021/1‑94 (MQ=60)
                                                     CCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGT                                         >  SRR4302536.2659214/1‑94 (MQ=60)
                                                     CCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGT                                         <  SRR4302536.4267206/94‑1 (MQ=60)
                                                      CAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTG                                        <  SRR4302536.1086705/94‑1 (MQ=60)
                                                      CAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTG                                        <  SRR4302536.2910546/94‑1 (MQ=60)
                                                      CAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTG                                        <  SRR4302536.2210573/94‑1 (MQ=60)
                                                      CAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTG                                        >  SRR4302536.3433846/1‑94 (MQ=60)
                                                      CAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTG                                        >  SRR4302536.4040589/1‑94 (MQ=60)
                                                        AACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGAC                                      >  SRR4302536.2316312/1‑94 (MQ=60)
                                                        AACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGAC                                      <  SRR4302536.4686992/94‑1 (MQ=60)
                                                        AACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGAC                                      >  SRR4302536.2356116/1‑94 (MQ=60)
                                                         ACGGCGGCAAGAGTTTATTTTTCGCGTAGCCAAAGCACTCTTCACCCAAATACCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACT                                     <  SRR4302536.4463366/94‑1 (MQ=60)
                                                         ACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTTCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACT                                     <  SRR4302536.526151/94‑1 (MQ=60)
                                                         ACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACT                                     <  SRR4302536.1653725/94‑1 (MQ=60)
                                                            tcgtCCAGAGTTTATTTTTCGCGTAGCCTCTGCTCTCTTCACCCAAATTCCAGCGTTCAGCGATGGCATATGAGCCACCAGCCAGGTGAATCAG                                  <  SRR4302536.6152388/90‑1 (MQ=57)
                                                             CGACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGC                                 >  SRR4302536.3130375/1‑94 (MQ=60)
                                                             CGACCAGAGATTATTTTTCGCGTAGCCCCAGCACTCTTCTGCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTAACTCAGC                                 >  SRR4302536.703470/1‑94 (MQ=60)
                                                              GACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCT                                >  SRR4302536.3569184/1‑94 (MQ=60)
                                                               ACCAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTC                               >  SRR4302536.4997019/1‑94 (MQ=60)
                                                                 CAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAC                             >  SRR4302536.4902953/1‑94 (MQ=60)
                                                                 CAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAC                             >  SRR4302536.2275294/1‑94 (MQ=60)
                                                                 CAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAC                             >  SRR4302536.1522130/1‑94 (MQ=60)
                                                                 CAGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAC                             >  SRR4302536.1464975/1‑94 (MQ=60)
                                                                  AGAGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACC                            >  SRR4302536.6000405/1‑94 (MQ=60)
                                                                    AGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCA                          >  SRR4302536.4647560/1‑94 (MQ=60)
                                                                    AGTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCA                          >  SRR4302536.5604841/1‑94 (MQ=60)
                                                                     GTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCAC                         >  SRR4302536.5168080/1‑94 (MQ=60)
                                                                     GTTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCAC                         <  SRR4302536.181526/94‑1 (MQ=60)
                                                                     GTTTATTTTTCGCGTAGCCCCAGCAATCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCAC                         >  SRR4302536.6655210/1‑94 (MQ=60)
                                                                      TTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACA                        <  SRR4302536.1125905/94‑1 (MQ=60)
                                                                      TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACA                        >  SRR4302536.3399338/1‑94 (MQ=60)
                                                                      TTTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACA                        <  SRR4302536.3943283/94‑1 (MQ=60)
                                                                       TTATTTTTCGCGTAGCCGCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACAC                       <  SRR4302536.2054621/94‑1 (MQ=60)
                                                                       TTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACAC                       <  SRR4302536.1404408/94‑1 (MQ=60)
                                                                       TTATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACAC                       >  SRR4302536.1408899/1‑94 (MQ=60)
                                                                        TATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACG                      >  SRR4302536.5547756/1‑94 (MQ=60)
                                                                         ATTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGC                     >  SRR4302536.1571863/1‑94 (MQ=60)
                                                                          TTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGAT                    <  SRR4302536.236562/94‑1 (MQ=60)
                                                                          TTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGAT                    <  SRR4302536.3645189/94‑1 (MQ=60)
                                                                          TTTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGAT                    <  SRR4302536.6675965/94‑1 (MQ=60)
                                                                           TTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATG                   <  SRR4302536.6196226/94‑1 (MQ=60)
                                                                           TTTTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATG                   <  SRR4302536.2925266/94‑1 (MQ=60)
                                                                             TTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCG                 <  SRR4302536.5749197/94‑1 (MQ=60)
                                                                             TTCGCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCG                 <  SRR4302536.2976317/94‑1 (MQ=60)
                                                                                GCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCA              <  SRR4302536.2086474/94‑1 (MQ=60)
                                                                                GCGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCA              <  SRR4302536.6618153/94‑1 (MQ=60)
                                                                                 TGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAG             >  SRR4302536.1798193/1‑94 (MQ=60)
                                                                                 CGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAG             >  SRR4302536.6856947/1‑94 (MQ=60)
                                                                                 CGTAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAG             >  SRR4302536.4016805/1‑94 (MQ=60)
                                                                                   TAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAC           <  SRR4302536.2213711/94‑1 (MQ=60)
                                                                                   TAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAC           >  SRR4302536.6456768/1‑94 (MQ=60)
                                                                                   GAGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGAC           <  SRR4302536.5583828/94‑1 (MQ=60)
                                                                                    AGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACG          <  SRR4302536.1946320/94‑1 (MQ=60)
                                                                                    AGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACG          <  SRR4302536.4471986/94‑1 (MQ=60)
                                                                                    AGCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACG          <  SRR4302536.3885336/94‑1 (MQ=60)
                                                                                     GCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGG         >  SRR4302536.6513542/1‑94 (MQ=60)
                                                                                     GCCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGG         <  SRR4302536.3846616/94‑1 (MQ=60)
                                                                                      CCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGT        <  SRR4302536.6277956/94‑1 (MQ=60)
                                                                                      CCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGT        <  SRR4302536.933576/94‑1 (MQ=60)
                                                                                      CCCCAGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGT        <  SRR4302536.326799/94‑1 (MQ=60)
                                                                                          AGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTA    <  SRR4302536.4578705/94‑1 (MQ=60)
                                                                                          AGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTA    <  SRR4302536.6448480/94‑1 (MQ=60)
                                                                                          AGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTA    <  SRR4302536.1991152/94‑1 (MQ=60)
                                                                                          AGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTA    >  SRR4302536.5133377/1‑94 (MQ=60)
                                                                                          AGCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTA    >  SRR4302536.1781286/1‑94 (MQ=60)
                                                                                           GCACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCGAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAA   <  SRR4302536.5335813/94‑1 (MQ=60)
                                                                                            CACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAAA  >  SRR4302536.40595/1‑94 (MQ=60)
                                                                                            CACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAAA  >  SRR4302536.4178110/1‑94 (MQ=60)
                                                                                            CACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAAA  >  SRR4302536.4302469/1‑94 (MQ=60)
                                                                                            CACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAAA  <  SRR4302536.682505/94‑1 (MQ=60)
                                                                                            |                                                                                             
TAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCACCCACACGATGCGCCAGACGGTAGTAAA  >  NC_000913/1750399‑1750584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑