Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,776,191 T→C R325R (CGT→CGC)  b1695 → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,776,1910TC99.3% 442.3 / ‑4.4 140R325R (CGT→CGC) b1695putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (70/69);  total (71/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.71e-01

Only 100 of 147 total aligned reads displayed.

GCGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGA  >  NC_000913/1776101‑1776280
                                                                                          |                                                                                         
 cgcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCAcc                                                                                        >  2:1848595/1‑93 (MQ=255)
 cgcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCAcc                                                                                        <  2:1829512/93‑1 (MQ=255)
  gcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGGACGCACCg                                                                                       >  2:1828507/1‑93 (MQ=255)
  gcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCg                                                                                       >  1:3900612/1‑93 (MQ=255)
  gcAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCg                                                                                       >  1:3837315/1‑93 (MQ=255)
    aaCATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCa                                                                                     >  1:1292667/1‑93 (MQ=255)
    aaCATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCa                                                                                     >  2:1768767/1‑93 (MQ=255)
    aaCATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCa                                                                                     >  1:3299042/1‑93 (MQ=255)
     aCATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCaa                                                                                    <  1:113678/93‑1 (MQ=255)
     aCATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCaa                                                                                    >  2:3528224/1‑93 (MQ=255)
      cATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAAt                                                                                   <  1:1843205/93‑1 (MQ=255)
       aTGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATg                                                                                  >  2:1889216/1‑93 (MQ=255)
       aTGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATg                                                                                  >  1:2154334/1‑93 (MQ=255)
       aTGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATg                                                                                  <  2:1334973/93‑1 (MQ=255)
       aTGGTGCTGAAAGTGGCATGACAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATg                                                                                  <  1:1933100/93‑1 (MQ=255)
        tGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATgg                                                                                 <  2:3741987/93‑1 (MQ=255)
         ggTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGa                                                                                >  2:1918331/1‑93 (MQ=255)
          gTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGaa                                                                               >  2:4387822/1‑93 (MQ=255)
          gTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGaa                                                                               <  2:3064306/93‑1 (MQ=255)
          gTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGaa                                                                               >  1:301288/1‑93 (MQ=255)
            gCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGt                                                                             >  2:3794162/1‑93 (MQ=255)
             cTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTc                                                                            <  1:646258/93‑1 (MQ=255)
              tGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCa                                                                           >  1:4632804/1‑93 (MQ=255)
               gAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAt                                                                          >  1:4071293/1‑93 (MQ=255)
               gAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAt                                                                          <  1:3500158/93‑1 (MQ=255)
                aaaGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAtt                                                                         <  2:319247/93‑1 (MQ=255)
                aaaGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAtt                                                                         >  1:3106366/1‑93 (MQ=255)
                aaaGTGGCATGGCAAGCCGATCAGCAGCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAtt                                                                         <  1:3766872/93‑1 (MQ=255)
                  aGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtga                                                                       <  2:2504172/93‑1 (MQ=255)
                  aGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtga                                                                       <  2:3289579/93‑1 (MQ=255)
                     ggcatggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtgatga                                                                    <  1:3762710/93‑1 (MQ=255)
                     ggcatggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATtgatga                                                                    <  1:651194/93‑1 (MQ=255)
                      gcatggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTgatgat                                                                   <  2:3889188/93‑1 (MQ=255)
                       catggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGatgatg                                                                  <  1:2002679/93‑1 (MQ=255)
                        atggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGc                                                                 >  1:1910151/1‑93 (MQ=255)
                        atggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGc                                                                 >  1:4623882/1‑93 (MQ=255)
                         tggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCg                                                                >  2:4675393/1‑93 (MQ=255)
                         tggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCg                                                                >  1:2687587/1‑93 (MQ=255)
                         tggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAACTCATTGATGATGCg                                                                >  2:2163837/1‑93 (MQ=255)
                         tcgcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCg                                                                <  2:13061/91‑1 (MQ=255)
                          ggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGa                                                               >  1:909223/1‑93 (MQ=255)
                          ggcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGa                                                               <  1:4514105/93‑1 (MQ=255)
                           gcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGAt                                                              <  2:2414247/93‑1 (MQ=255)
                           gcaAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGAt                                                              >  1:4746746/1‑93 (MQ=255)
                             aaGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTc                                                            >  1:8015/1‑93 (MQ=255)
                             aaGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTc                                                            >  2:417820/1‑93 (MQ=255)
                                ccGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCaaa                                                         <  2:4365683/93‑1 (MQ=255)
                                  gATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATc                                                       >  2:2428403/1‑93 (MQ=255)
                                  gATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATCGATGATGCGATTCAAATc                                                       <  1:165773/93‑1 (MQ=255)
                                   aTCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCa                                                      <  2:3382869/93‑1 (MQ=255)
                                   aTCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCa                                                      >  2:4697014/1‑93 (MQ=255)
                                    tCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCAt                                                     >  2:620216/1‑93 (MQ=255)
                                        cATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGc                                                 <  1:2064572/93‑1 (MQ=255)
                                        cATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGc                                                 >  2:1748940/1‑93 (MQ=255)
                                         aTCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCATTGGAAGTCATTGATGATGCGATTCAAATCATGGGCg                                                <  2:2946463/93‑1 (MQ=255)
                                          tCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCgg                                               >  2:4016778/1‑93 (MQ=255)
                                           cAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGt                                              <  1:2616144/93‑1 (MQ=255)
                                            aGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTc                                             >  2:2743064/1‑93 (MQ=255)
                                            aGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTc                                             >  1:756851/1‑93 (MQ=255)
                                              tCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTg                                           <  1:1653545/93‑1 (MQ=255)
                                                aCTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTggg                                         >  1:2492415/1‑93 (MQ=255)
                                                 cTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTgggt                                        >  1:2704594/1‑92 (MQ=255)
                                                   gcgcACCAGCGCTGCGCTGGCAAAGCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCta                                      <  2:681007/93‑1 (MQ=255)
                                                   gcgcACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCta                                      <  2:681008/93‑1 (MQ=255)
                                                     gcACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCtata                                    <  1:1164949/93‑1 (MQ=255)
                                                     gcACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCtata                                    <  2:3507249/93‑1 (MQ=255)
                                                      cACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATAc                                   >  2:1843406/1‑93 (MQ=255)
                                                         cAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGa                                <  1:1494845/93‑1 (MQ=255)
                                                          aGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGAt                               <  2:418651/93‑1 (MQ=255)
                                                          aGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGAt                               >  1:4316510/1‑93 (MQ=255)
                                                           gcgcGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATg                              <  2:4587908/93‑1 (MQ=255)
                                                             gcggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAg                            <  1:3807607/93‑1 (MQ=255)
                                                             gcggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAg                            >  2:2526301/1‑93 (MQ=255)
                                                              cggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAgg                           >  2:4517502/1‑93 (MQ=255)
                                                              cggcgCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAgg                           >  1:616289/1‑93 (MQ=255)
                                                                  gcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgcg                       >  2:2880153/1‑93 (MQ=255)
                                                                  gcTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgcg                       <  2:1287825/93‑1 (MQ=255)
                                                                   cTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGgcgcgc                      >  2:3404589/1‑93 (MQ=255)
                                                                      gCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGtc                   <  2:62073/93‑1 (MQ=255)
                                                                         aaaCTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTcc                >  1:3021447/1‑93 (MQ=255)
                                                                           actgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCg              >  1:1198249/1‑93 (MQ=255)
                                                                           actgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCg              <  1:2758890/93‑1 (MQ=255)
                                                                           actgtactgtGCACGCACCGCAAGGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCg              >  2:2307115/1‑93 (MQ=255)
                                                                            ctgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGc             <  1:1580661/93‑1 (MQ=255)
                                                                            ctgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGc             >  2:1027357/1‑93 (MQ=255)
                                                                             tgtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCt            >  2:3725775/1‑93 (MQ=255)
                                                                              gtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCtt           <  1:2512241/93‑1 (MQ=255)
                                                                              gtactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCtt           >  1:3554546/1‑93 (MQ=255)
                                                                               tactgtGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTc          >  2:4515330/1‑93 (MQ=255)
                                                                               tactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTc          <  1:4437879/93‑1 (MQ=255)
                                                                               tactgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTc          <  2:2466971/93‑1 (MQ=255)
                                                                                actgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCt         >  1:3102106/1‑93 (MQ=255)
                                                                                 ctgtGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTg        <  2:515458/93‑1 (MQ=255)
                                                                                  tgtgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTgg       >  1:2743279/1‑93 (MQ=255)
                                                                                  tgtgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTgg       <  2:3246823/93‑1 (MQ=255)
                                                                                   gtgCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGc      >  1:1057550/1‑93 (MQ=255)
                                                                                     gcacgcacCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGt    >  1:4256886/1‑93 (MQ=255)
                                                                                      cACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTg   >  1:3550392/1‑93 (MQ=255)
                                                                                      cACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTg   >  1:4180356/1‑93 (MQ=255)
                                                                                      cACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTg   >  2:2725715/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGA  >  NC_000913/1776101‑1776280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTC  >  NC_000913/1776099‑1776284
                                                                                            |                                                                                             
ATGCGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCA                                                                                              >  SRR4302544.1107989/1‑94 (MQ=60)
   CGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCG                                                                                           >  SRR4302544.3875368/1‑94 (MQ=60)
   CGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCG                                                                                           >  SRR4302544.3939357/1‑94 (MQ=60)
    GCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGC                                                                                          <  SRR4302544.1604959/94‑1 (MQ=60)
     CAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCA                                                                                         >  SRR4302544.3330466/1‑94 (MQ=60)
     CAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCA                                                                                         >  SRR4302544.1305386/1‑94 (MQ=60)
     CAACATGGGTCTGAAAGTGGCATGACAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCA                                                                                         <  SRR4302544.1206277/94‑1 (MQ=60)
       ACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAAT                                                                                       <  SRR4302544.114714/94‑1 (MQ=60)
        CATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATG                                                                                      >  SRR4302544.2174821/1‑94 (MQ=60)
        CATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATG                                                                                      <  SRR4302544.1861072/94‑1 (MQ=60)
         ATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGG                                                                                     <  SRR4302544.1951810/94‑1 (MQ=60)
         ATGGTGCTGAAAGTGGCATGACAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGG                                                                                     <  SRR4302544.1951811/94‑1 (MQ=60)
          agggcCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGA                                                                                    <  SRR4302544.1077927/89‑1 (MQ=60)
           GGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAA                                                                                   >  SRR4302544.304410/1‑94 (MQ=60)
               CTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCA                                                                               >  SRR4302544.4678724/1‑94 (MQ=60)
               CTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCA                                                                               <  SRR4302544.652640/94‑1 (MQ=60)
               CTGAAAGTGGCATGGACAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCA                                                                               <  SRR4302544.4236015/94‑1 (MQ=60)
                TGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCAT                                                                              >  SRR4302544.4111815/1‑94 (MQ=60)
                 GAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATT                                                                             <  SRR4302544.3533549/94‑1 (MQ=60)
                 GAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATT                                                                             >  SRR4302544.3135937/1‑94 (MQ=60)
                  AAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTG                                                                            <  SRR4302544.1437921/94‑1 (MQ=60)
                  AAAGTGGCATGGCAAGCCGATCAGCAGCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTG                                                                            <  SRR4302544.3804229/94‑1 (MQ=60)
                      TGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGA                                                                        <  SRR4302544.864111/94‑1 (MQ=60)
                       GGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGAT                                                                       <  SRR4302544.657641/94‑1 (MQ=60)
                       GGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGAT                                                                       <  SRR4302544.3952340/94‑1 (MQ=60)
                       GGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGAT                                                                       <  SRR4302544.3800051/94‑1 (MQ=60)
                       GGCATGGAAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGAT                                                                       <  SRR4302544.1817994/94‑1 (MQ=60)
                         CATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGC                                                                     <  SRR4302544.2022130/94‑1 (MQ=60)
                         CATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGC                                                                     >  SRR4302544.4669751/1‑94 (MQ=60)
                         CATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGC                                                                     >  SRR4302544.4669750/1‑94 (MQ=60)
                         CATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGC                                                                     >  SRR4302544.1928611/1‑94 (MQ=60)
                          ATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCG                                                                    >  SRR4302544.2713350/1‑94 (MQ=60)
                           TGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGA                                                                   >  SRR4302544.918210/1‑94 (MQ=60)
                            GGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGAT                                                                  >  SRR4302544.4793510/1‑94 (MQ=60)
                            GGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGAT                                                                  <  SRR4302544.4559322/94‑1 (MQ=60)
                              CAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTC                                                                >  SRR4302544.8089/1‑94 (MQ=60)
                                 GCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAA                                                             >  SRR4302544.2955566/1‑94 (MQ=60)
                                   CGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATC                                                           <  SRR4302544.3191927/94‑1 (MQ=60)
                                    GATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATCGATGATGCGATTCAAATCA                                                          <  SRR4302544.167400/94‑1 (MQ=60)
                                       CAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGG                                                       >  SRR4302544.2075627/1‑94 (MQ=60)
                                          CATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCG                                                    <  SRR4302544.2084415/94‑1 (MQ=60)
                                             CAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTC                                                 >  SRR4302544.764239/1‑94 (MQ=60)
                                             CAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTC                                                 <  SRR4302544.2641254/94‑1 (MQ=60)
                                               GTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTG                                               >  SRR4302544.3645866/1‑94 (MQ=60)
                                                TCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGG                                              <  SRR4302544.1669853/94‑1 (MQ=60)
                                                 CACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGG                                             >  SRR4302544.2516283/1‑94 (MQ=60)
                                                  ACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGT                                            >  SRR4302544.2730596/1‑94 (MQ=60)
                                                   CTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCT                                           <  SRR4302544.3106713/94‑1 (MQ=60)
                                                       GCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATAC                                       <  SRR4302544.1176559/94‑1 (MQ=60)
                                                       GCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATAC                                       <  SRR4302544.4763293/94‑1 (MQ=60)
                                                        CACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACC                                      <  SRR4302544.4427573/94‑1 (MQ=60)
                                                           CAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGAT                                   <  SRR4302544.1509627/94‑1 (MQ=60)
                                                           CAGCGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGAT                                   >  SRR4302544.4360479/1‑94 (MQ=60)
                                                              CGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAG                                <  SRR4302544.625311/94‑1 (MQ=60)
                                                              CGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAG                                <  SRR4302544.757261/94‑1 (MQ=60)
                                                              CGCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATAATGGGCGGTCTGGGCTATACCGATGAG                                <  SRR4302544.3555401/94‑1 (MQ=60)
                                                               GCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGG                               <  SRR4302544.3845341/94‑1 (MQ=60)
                                                               GCGGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGG                               >  SRR4302544.622339/1‑94 (MQ=60)
                                                                 GGCGCTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCG                             <  SRR4302544.2697074/94‑1 (MQ=60)
                                                                     CTGGCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCG                         >  SRR4302544.3839916/1‑94 (MQ=60)
                                                                        GCAAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCT                      >  SRR4302544.2767718/1‑94 (MQ=60)
                                                                          AAAACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCC                    >  SRR4302544.3050402/1‑94 (MQ=60)
                                                                            AACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCG                  >  SRR4302544.1210126/1‑94 (MQ=60)
                                                                             ACTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGC                 <  SRR4302544.2785440/94‑1 (MQ=60)
                                                                              CTGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCT                <  SRR4302544.1596283/94‑1 (MQ=60)
                                                                               TGTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTT               >  SRR4302544.3588603/1‑94 (MQ=60)
                                                                                GTACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTC              <  SRR4302544.2536353/94‑1 (MQ=60)
                                                                                 TACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCT             <  SRR4302544.4482628/94‑1 (MQ=60)
                                                                                 TACTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCT             >  SRR4302544.3131633/1‑94 (MQ=60)
                                                                                   CTGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGG           >  SRR4302544.2769717/1‑94 (MQ=60)
                                                                                    TGTGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGC          >  SRR4302544.1068047/1‑94 (MQ=60)
                                                                                      TGCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGT        >  SRR4302544.4300285/1‑94 (MQ=60)
                                                                                       GCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTG       >  SRR4302544.4221757/1‑94 (MQ=60)
                                                                                       GCACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTG       >  SRR4302544.3584396/1‑94 (MQ=60)
                                                                                        CACGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGA      >  SRR4302544.2225275/1‑94 (MQ=60)
                                                                                          CGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATG    >  SRR4302544.269119/1‑94 (MQ=60)
                                                                                          CGCACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATG    <  SRR4302544.3628895/94‑1 (MQ=60)
                                                                                            CACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTC  >  SRR4302544.535457/1‑94 (MQ=60)
                                                                                            CACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTC  >  SRR4302544.541026/1‑94 (MQ=60)
                                                                                            |                                                                                             
ATGCGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAGCATCAGTCACTGCGCACCAGCGCGGCGCTGGCAAAACTGTACTGTGCACGTACCGCAATGGAAGTCATTGATGATGCGATTCAAATCATGGGCGGTCTGGGCTATACCGATGAGGCGCGCGTCTCCCGCTTCTGGCGTGATGTC  >  NC_000913/1776099‑1776284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑