Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,799,569 G→A N342N (AAC→AAT)  thrS ← threonine tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,799,5690GA100.0% 464.7 / NA 134N342N (AAC→AAT) thrSthreonine tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (62/72);  total (62/72)

Only 100 of 137 total aligned reads displayed.

GCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTA  >  NC_000913/1799479‑1799658
                                                                                          |                                                                                         
gCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGAtt                                                                                         <  2:3048275/93‑1 (MQ=255)
gCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGAtt                                                                                         <  1:490639/93‑1 (MQ=255)
gCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGAtt                                                                                         >  2:1877197/1‑93 (MQ=255)
  cATGCAGCGAACCTGACGGCTCGTTACGGGGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGa                                                                                       >  1:327230/1‑93 (MQ=255)
   aTGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGaa                                                                                      >  1:3624784/1‑93 (MQ=255)
      cAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATATGACTTCAGCCCCTGATTGAAAAt                                                                                   >  2:2599222/1‑93 (MQ=255)
       aGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAAtt                                                                                  >  2:4574347/1‑93 (MQ=255)
        gCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAAttt                                                                                 <  2:2643191/93‑1 (MQ=255)
        gCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAAttt                                                                                 <  2:491977/93‑1 (MQ=255)
        gCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAAttt                                                                                 >  2:463568/1‑93 (MQ=255)
           aaCCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTa                                                                              <  2:2629514/93‑1 (MQ=255)
           aaCCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTa                                                                              <  1:1622775/93‑1 (MQ=255)
           aaCCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTa                                                                              <  2:3410436/93‑1 (MQ=255)
           aaCCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGAAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTa                                                                              <  1:2567889/93‑1 (MQ=255)
            aCCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTAc                                                                             >  2:4548847/1‑93 (MQ=255)
             ccTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACg                                                                            >  1:127719/1‑93 (MQ=255)
             ccTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACg                                                                            >  1:1259751/1‑93 (MQ=255)
                gACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGa                                                                         >  1:4416818/1‑93 (MQ=255)
                 aCGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGAc                                                                        <  2:643879/93‑1 (MQ=255)
                   ggCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGAccc                                                                      >  1:4256815/1‑93 (MQ=255)
                    gCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCg                                                                     <  1:2705523/93‑1 (MQ=255)
                     cTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCgg                                                                    <  2:1356068/93‑1 (MQ=255)
                     cTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCgg                                                                    >  1:458836/1‑93 (MQ=255)
                           aCGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGtt                                                              <  2:3255569/93‑1 (MQ=255)
                            cGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTc                                                             <  1:1379169/93‑1 (MQ=255)
                             ggTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCa                                                            <  1:3411472/93‑1 (MQ=255)
                               gggCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGCCTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATc                                                          <  2:338469/92‑1 (MQ=255)
                                ggCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCg                                                         >  2:3726588/1‑93 (MQ=255)
                                 gCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCgg                                                        <  1:2366738/93‑1 (MQ=255)
                                  cAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGc                                                       <  1:2127049/93‑1 (MQ=255)
                                   aGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCt                                                      >  2:2183141/1‑93 (MQ=255)
                                   aGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCt                                                      <  1:4162543/93‑1 (MQ=255)
                                    gCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCtt                                                     <  1:1550174/93‑1 (MQ=255)
                                     cTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTa                                                    >  2:4091332/1‑93 (MQ=255)
                                     cTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTa                                                    <  2:1247757/93‑1 (MQ=255)
                                      tACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTaa                                                   <  1:4471898/93‑1 (MQ=255)
                                      tACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTaa                                                   <  2:1274268/93‑1 (MQ=255)
                                       aCCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAAt                                                  >  1:1359946/1‑93 (MQ=255)
                                       aCCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAAt                                                  <  1:995754/93‑1 (MQ=255)
                                          aaaCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCa                                               >  2:937755/1‑93 (MQ=255)
                                          aaaCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCa                                               >  1:2119097/1‑93 (MQ=255)
                                           aaCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAg                                              <  2:2540429/93‑1 (MQ=255)
                                           aaCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAg                                              <  2:2499105/93‑1 (MQ=255)
                                           aaCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAg                                              >  2:4411207/1‑93 (MQ=255)
                                             cTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTa                                            <  1:3360187/93‑1 (MQ=255)
                                              tCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTAt                                           <  1:358020/93‑1 (MQ=255)
                                                ggCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTc                                         <  2:3462191/93‑1 (MQ=255)
                                                  ccATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCAc                                       <  1:3941594/93‑1 (MQ=255)
                                                  ccATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCAc                                       <  2:3878600/93‑1 (MQ=255)
                                                  ccATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCAc                                       <  2:4285691/93‑1 (MQ=255)
                                                   cATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACg                                      <  2:4512762/93‑1 (MQ=255)
                                                   cATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTAATCGGCTTAATGCAGTATTCACg                                      >  1:1188066/1‑93 (MQ=255)
                                                    aTACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACgg                                     >  2:26520/1‑93 (MQ=255)
                                                    aTACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACgg                                     >  1:1941224/1‑93 (MQ=255)
                                                    aTACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACgg                                     <  2:4543754/93‑1 (MQ=255)
                                                     tACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGt                                    >  2:136518/1‑93 (MQ=255)
                                                      aCGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTCCGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGtt                                   <  1:1616809/93‑1 (MQ=255)
                                                        gCNGCGGCAGATCGCGATAAGNNTTNAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTtct                                 <  1:696877/93‑1 (MQ=255)
                                                        gCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTtct                                 >  2:2804176/1‑93 (MQ=255)
                                                        gCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTtct                                 <  2:54412/93‑1 (MQ=255)
                                                         cAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTtctc                                >  1:451651/1‑93 (MQ=255)
                                                          aGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACTCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCa                               <  2:366023/93‑1 (MQ=255)
                                                            cGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCaga                             <  1:482722/93‑1 (MQ=255)
                                                            cGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCaga                             >  2:1520804/1‑93 (MQ=255)
                                                             ggCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCagaa                            >  1:515627/1‑93 (MQ=255)
                                                             ggCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCagaa                            <  2:1246117/93‑1 (MQ=255)
                                                                aGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGTCTTAATGCAGTATTCAAGGTTCTCagaagag                         >  1:3948065/1‑92 (MQ=255)
                                                                aGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAt                         <  2:4480681/93‑1 (MQ=255)
                                                                aGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAt                         >  1:3948066/1‑93 (MQ=255)
                                                                 gATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCGCGGTTCTCAGAAGAtg                        >  1:1738011/1‑93 (MQ=255)
                                                                 gATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAtg                        >  2:3744610/1‑93 (MQ=255)
                                                                 gATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAtg                        >  2:25474/1‑93 (MQ=255)
                                                                  aTCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAtgt                       <  2:876076/93‑1 (MQ=255)
                                                                  aTCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAtgt                       <  2:836086/93‑1 (MQ=255)
                                                                  aTCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAtgt                       >  1:196868/1‑93 (MQ=255)
                                                                   tCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAtgtg                      <  1:3096884/93‑1 (MQ=255)
                                                                    cgcgATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATgtgg                     <  1:2815787/93‑1 (MQ=255)
                                                                     gcgATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATgtggt                    <  1:4477437/93‑1 (MQ=255)
                                                                     gcgATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATgtggt                    <  1:3001627/93‑1 (MQ=255)
                                                                        aTAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGaa                 >  2:3170872/1‑93 (MQ=255)
                                                                        aTAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGaa                 >  1:297177/1‑93 (MQ=255)
                                                                           aGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACAt              >  1:539695/1‑93 (MQ=255)
                                                                           aGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACAt              >  2:2017173/1‑93 (MQ=255)
                                                                            gACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACAtt             <  1:1264455/93‑1 (MQ=255)
                                                                            gACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACAtt             >  1:200634/1‑93 (MQ=255)
                                                                             aCTTAAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGATCATCGGCTTCATGCAGTATTCACGGTCCTCAGAAGATGTGGTGAACAATg            >  2:2586442/1‑93 (MQ=255)
                                                                               ttCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCa          >  2:4137876/1‑93 (MQ=255)
                                                                               ttCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCa          <  1:4635096/93‑1 (MQ=255)
                                                                                 cAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATc        >  2:2209731/1‑93 (MQ=255)
                                                                                 cAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATc        <  2:1010268/93‑1 (MQ=255)
                                                                                   gCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCtt      >  2:3911393/1‑93 (MQ=255)
                                                                                   gCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCtt      >  1:579041/1‑93 (MQ=255)
                                                                                   gCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCtt      >  2:685553/1‑93 (MQ=255)
                                                                                   gCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCtt      >  1:2115730/1‑93 (MQ=255)
                                                                                    ccccTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCttt     <  2:4453707/93‑1 (MQ=255)
                                                                                    ccccTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCttt     <  2:3350015/93‑1 (MQ=255)
                                                                                      ccTGATTGAATATTTGTACGTGACCCGGGCAGTGCATTGGCGTAATGCAGTATTCACGCTGCTCAGGAGATGTGGTGAACATTGCATCTTTGt   <  2:3927549/93‑1 (MQ=255)
                                                                                      ccTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGt   >  1:2499797/1‑93 (MQ=255)
                                                                                       cTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTa  <  1:185788/93‑1 (MQ=255)
                                                                                       cTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTa  >  2:2188221/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTA  >  NC_000913/1799479‑1799658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTT  >  NC_000913/1799476‑1799661
                                                                                             |                                                                                            
CAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGA                                                                                              >  SRR4302544.2544716/1‑94 (MQ=60)
CAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGA                                                                                              >  SRR4302544.2544564/1‑94 (MQ=60)
CAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGA                                                                                              >  SRR4302544.2096828/1‑94 (MQ=60)
CAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGA                                                                                              <  SRR4302544.4307539/94‑1 (MQ=60)
 AGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGAT                                                                                             >  SRR4302544.1941747/1‑94 (MQ=60)
  GGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATT                                                                                            <  SRR4302544.3907259/94‑1 (MQ=60)
  GGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATT                                                                                            <  SRR4302544.1894346/94‑1 (MQ=60)
   GCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTG                                                                                           <  SRR4302544.495459/94‑1 (MQ=60)
    CCATGCAGCGAACCTGACGGCTCGTTACGGGGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGA                                                                                          >  SRR4302544.330634/1‑94 (MQ=60)
     CATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAA                                                                                         >  SRR4302544.3660902/1‑94 (MQ=60)
              AACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTAC                                                                                <  SRR4302544.1638762/94‑1 (MQ=60)
              AACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGAAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTAC                                                                                <  SRR4302544.2592559/94‑1 (MQ=60)
               ACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACG                                                                               >  SRR4302544.128895/1‑94 (MQ=60)
               ACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACG                                                                               >  SRR4302544.1272145/1‑94 (MQ=60)
                CCTGACGGCTCGTTACGGTGGCAGCTACCAACCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGT                                                                              <  SRR4302544.3509259/94‑1 (MQ=60)
                 CTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTG                                                                             >  SRR4302544.191965/1‑94 (MQ=60)
                 CTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTG                                                                             >  SRR4302544.2574066/1‑94 (MQ=60)
                  TGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGA                                                                            >  SRR4302544.4461401/1‑94 (MQ=60)
                    ACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACC                                                                          <  SRR4302544.3153105/94‑1 (MQ=60)
                     CGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCC                                                                         >  SRR4302544.4300214/1‑94 (MQ=60)
                       GCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGG                                                                       >  SRR4302544.463349/1‑94 (MQ=60)
                       GCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGG                                                                       <  SRR4302544.2731540/94‑1 (MQ=60)
                         TCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGC                                                                     <  SRR4302544.4532117/94‑1 (MQ=60)
                               CGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCA                                                               <  SRR4302544.1392938/94‑1 (MQ=60)
                                GGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCAT                                                              <  SRR4302544.3444078/94‑1 (MQ=60)
                                    GCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGC                                                          <  SRR4302544.2389132/94‑1 (MQ=60)
                                     CAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCT                                                         <  SRR4302544.2147443/94‑1 (MQ=60)
                                      AGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTT                                                        <  SRR4302544.4203777/94‑1 (MQ=60)
                                       GCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTA                                                       <  SRR4302544.1565616/94‑1 (MQ=60)
                                         TACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAAT                                                     >  SRR4302544.1373448/1‑94 (MQ=60)
                                         TACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAAT                                                     <  SRR4302544.4516873/94‑1 (MQ=60)
                                          ACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATG                                                    <  SRR4302544.1005603/94‑1 (MQ=60)
                                           CCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGC                                                   >  SRR4302544.2079327/1‑94 (MQ=60)
                                            CAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCA                                                  >  SRR4302544.2139442/1‑94 (MQ=60)
                                             AAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAG                                                 <  SRR4302544.2846247/94‑1 (MQ=60)
                                              AACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGT                                                >  SRR4302544.128491/1‑94 (MQ=60)
                                                CTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTAT                                              <  SRR4302544.3392252/94‑1 (MQ=60)
                                                 TCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATT                                             <  SRR4302544.361692/94‑1 (MQ=60)
                                                     CCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACG                                         <  SRR4302544.3980754/94‑1 (MQ=60)
                                                     CCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACG                                         >  SRR4302544.1663653/1‑94 (MQ=60)
                                                     CCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTAATCGGCTTAATGCAGTATTCACG                                         >  SRR4302544.1199864/1‑94 (MQ=60)
                                                      CATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGG                                        >  SRR4302544.1960010/1‑94 (MQ=60)
                                                         ACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTCCGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTC                                     <  SRR4302544.1632745/94‑1 (MQ=60)
                                                          CGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCT                                    <  SRR4302544.3926043/94‑1 (MQ=60)
                                                           GCNGCGGCAGATCGCGATAAGNNTTNAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTC                                   <  SRR4302544.703818/94‑1 (MQ=60)
                                                           GCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTC                                   >  SRR4302544.456128/1‑94 (MQ=60)
                                                             AGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAG                                 <  SRR4302544.4155053/94‑1 (MQ=60)
                                                               CGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAA                               >  SRR4302544.520723/1‑94 (MQ=60)
                                                               CGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAA                               <  SRR4302544.487451/94‑1 (MQ=60)
                                                                  CAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGTCTTAATGCAGTATTCAAGGTTCTCAGAAGAG                            >  SRR4302544.3987298/1‑94 (MQ=60)
                                                                  CAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAT                            >  SRR4302544.3987299/1‑94 (MQ=60)
                                                                   AGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCCGTTCATCGGCTTGATGCAGTATTCACGGTTCTCAGAAGCTG                           >  SRR4302544.2358235/1‑94 (MQ=60)
                                                                   AGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCGCGGTTCTCAGAAGATG                           >  SRR4302544.1755032/1‑94 (MQ=60)
                                                                   AGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATG                           <  SRR4302544.607728/94‑1 (MQ=60)
                                                                    GATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGT                          >  SRR4302544.198856/1‑94 (MQ=60)
                                                                      TCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGG                        <  SRR4302544.3126374/94‑1 (MQ=60)
                                                                       CGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGT                       <  SRR4302544.2842971/94‑1 (MQ=60)
                                                                        GCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTG                      <  SRR4302544.3030403/94‑1 (MQ=60)
                                                                        GCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTG                      <  SRR4302544.4522455/94‑1 (MQ=60)
                                                                         CGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGA                     <  SRR4302544.4778393/94‑1 (MQ=60)
                                                                          GATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAA                    >  SRR4302544.300249/1‑94 (MQ=60)
                                                                           ATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAAC                   >  SRR4302544.4508765/1‑94 (MQ=60)
                                                                             AAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACAT                 >  SRR4302544.545018/1‑94 (MQ=60)
                                                                              AGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATT                >  SRR4302544.202665/1‑94 (MQ=60)
                                                                               GACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTG               <  SRR4302544.1276894/94‑1 (MQ=60)
                                                                                 CTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCA             >  SRR4302544.1460563/1‑94 (MQ=60)
                                                                                  TTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCAT            <  SRR4302544.4681029/94‑1 (MQ=60)
                                                                                     AGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTT         >  SRR4302544.2136053/1‑94 (MQ=60)
                                                                                     AGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTT         >  SRR4302544.584699/1‑94 (MQ=60)
                                                                                        CCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGT      >  SRR4302544.2523761/1‑94 (MQ=60)
                                                                                          CTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAG    <  SRR4302544.187634/94‑1 (MQ=60)
                                                                                          CTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAG    >  SRR4302544.1331962/1‑94 (MQ=60)
                                                                                          CTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAG    <  SRR4302544.52568/94‑1 (MQ=60)
                                                                                          CTGATGGAAAATTTGTACGTGACCCGGTCAGGTCATCGGGGTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAG    <  SRR4302544.187635/94‑1 (MQ=60)
                                                                                           TGATTGAAAATTTGTACGTGACCCGGGCAGGTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGT   >  SRR4302544.4643950/1‑94 (MQ=60)
                                                                                            GATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTT  >  SRR4302544.292939/1‑94 (MQ=60)
                                                                                             |                                                                                            
CAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTT  >  NC_000913/1799476‑1799661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑