Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,799,569	G→A	N342N (AAC→AAT)	thrS ←	threonine tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,799,569	0	G	A	99.6%	886.3 / NA	237	N342N (AAC→AAT)	thrS	threonine tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (102/134); minor base C (1/0); total (103/134)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

GCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTA  >  NC_000913/1799479‑1799658

gCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGAtt                                                                                         >  1:4318020/1‑93 (MQ=255)

 ccATGAAACGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTg                                                                                        <  1:4097299/93‑1 (MQ=255)

   aTGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGaa                                                                                      >  2:4061208/1‑93 (MQ=255)

     gCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGaaaa                                                                                    <  1:4309068/93‑1 (MQ=255)

       aGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAAtt                                                                                  <  1:3646996/93‑1 (MQ=255)

       aGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAAtt                                                                                  <  2:1047851/93‑1 (MQ=255)

       aGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAAtt                                                                                  <  2:5542960/93‑1 (MQ=255)

        gCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAAttt                                                                                 >  2:1982839/1‑93 (MQ=255)

        gCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAAttt                                                                                 <  2:1741287/93‑1 (MQ=255)

           aaCGTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTa                                                                              <  1:5630914/93‑1 (MQ=255)

           aaCCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTa                                                                              >  2:3931731/1‑93 (MQ=255)

            aCCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTAc                                                                             <  1:2166151/93‑1 (MQ=255)

            aCCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTAc                                                                             >  1:4852003/1‑93 (MQ=255)

            aCCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTAc                                                                             <  1:688536/93‑1 (MQ=255)

             ccTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACg                                                                            <  1:5482706/93‑1 (MQ=255)

                gACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGa                                                                         >  1:477527/1‑93 (MQ=255)

                 aCGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGAc                                                                        <  1:12415/93‑1 (MQ=255)

                 aCGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGAc                                                                        >  1:1121503/1‑93 (MQ=255)

                     cTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCgg                                                                    <  2:637870/93‑1 (MQ=255)

                      tCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCggg                                                                   >  1:4963844/1‑93 (MQ=255)

                      tCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCggg                                                                   <  2:6182720/93‑1 (MQ=255)

                       cGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGc                                                                  <  1:2602537/93‑1 (MQ=255)

                         ttACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAg                                                                >  1:6277576/1‑93 (MQ=255)

                          tACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGt                                                               >  1:303582/1‑93 (MQ=255)

                             ggTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCa                                                            <  2:1861999/93‑1 (MQ=255)

                              gTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCAt                                                           <  2:5149314/93‑1 (MQ=255)

                              gTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCAt                                                           >  1:1297096/1‑93 (MQ=255)

                              gTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCAt                                                           >  1:5226893/1‑93 (MQ=255)

                               tGGCAGCTACCAATCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGCTCATc                                                          <  1:4221361/93‑1 (MQ=255)

                               tGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATc                                                          >  2:6589546/1‑93 (MQ=255)

                                 gCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCgg                                                        <  1:3273825/93‑1 (MQ=255)

                                 gCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCgg                                                        >  2:4234131/1‑93 (MQ=255)

                                 gCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCgg                                                        >  1:2830583/1‑93 (MQ=255)

                                 gCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCgg                                                        >  2:2996199/1‑93 (MQ=255)

                                 gCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCgg                                                        <  1:514500/93‑1 (MQ=255)

                                  cAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGc                                                       >  2:308477/1‑93 (MQ=255)

                                  cAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGc                                                       >  2:4666132/1‑93 (MQ=255)

                                   cgCTACCAAACTCGGCCATACGCAGCCGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCt                                                      <  2:1411593/92‑1 (MQ=255)

                                       aCCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAAt                                                  <  1:271111/93‑1 (MQ=255)

                                        ccAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATg                                                 >  2:5012847/1‑93 (MQ=255)

                                         cAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGc                                                <  2:5305831/93‑1 (MQ=255)

                                          aaaCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCa                                               >  2:2064855/1‑93 (MQ=255)

                                          aaaCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCa                                               <  1:3104847/93‑1 (MQ=255)

                                          aaaCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCa                                               <  2:3225153/93‑1 (MQ=255)

                                           aaCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAg                                              >  2:229166/1‑93 (MQ=255)

                                            aCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGt                                             <  2:1541709/93‑1 (MQ=255)

                                             cTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTa                                            <  2:4991104/93‑1 (MQ=255)

                                             cTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTa                                            <  2:250560/93‑1 (MQ=255)

                                              tCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTAt                                           <  2:4765180/93‑1 (MQ=255)

                                              tCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTAt                                           <  2:6152908/93‑1 (MQ=255)

                                              gcGGCCATACGCAGCGCCAGATCGCGATAAGACTTCAGCCTCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTAt                                           <  1:165386/92‑1 (MQ=255)

                                                 gCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCa                                        >  1:5015955/1‑93 (MQ=255)

                                                   cATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACg                                      <  2:125711/93‑1 (MQ=255)

                                                   cATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACg                                      >  1:1581559/1‑93 (MQ=255)

                                                    aTACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACgg                                     >  1:722030/1‑93 (MQ=255)

                                                     tACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGt                                    >  1:5850148/1‑93 (MQ=255)

                                                     tACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGt                                    >  2:5487487/1‑93 (MQ=255)

                                                     tACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGt                                    <  1:5201862/93‑1 (MQ=255)

                                                       cGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTtc                                  >  1:899623/1‑93 (MQ=255)

                                                       cGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTtc                                  <  1:2104512/93‑1 (MQ=255)

                                                         cAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTtctc                                <  1:1078235/93‑1 (MQ=255)

                                                           gCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCag                              >  2:565407/1‑93 (MQ=255)

                                                            cGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCaga                             <  1:3413614/93‑1 (MQ=255)

                                                               cAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCagaaga                          <  1:1451667/93‑1 (MQ=255)

                                                                 gATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAtg                        >  1:447098/1‑93 (MQ=255)

                                                                  aTCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAtgt                       >  2:3590286/1‑93 (MQ=255)

                                                                  aTCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAtgt                       <  1:2305662/93‑1 (MQ=255)

                                                                  aTCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAtgt                       <  2:3432219/93‑1 (MQ=255)

                                                                  aTCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAtgt                       <  2:2116787/93‑1 (MQ=255)

                                                                   tCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAtgtg                      >  2:5948954/1‑93 (MQ=255)

                                                                   tCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAtgtg                      <  1:6014228/93‑1 (MQ=255)

                                                                   tCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAtgtg                      >  1:1029905/1‑93 (MQ=255)

                                                                     gggATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATgtggt                    <  2:5762065/91‑1 (MQ=255)

                                                                     gcgATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATgtggt                    >  2:776722/1‑93 (MQ=255)

                                                                       gATAAGACTCCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGa                  <  2:2689880/93‑1 (MQ=255)

                                                                        aTAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGaa                 <  2:3662826/93‑1 (MQ=255)

                                                                         tAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAAc                >  1:6205349/1‑93 (MQ=255)

                                                                         tAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAAc                >  1:6110736/1‑93 (MQ=255)

                                                                         tAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAAc                <  2:4011840/93‑1 (MQ=255)

                                                                         tAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAAc                <  2:5322800/93‑1 (MQ=255)

                                                                           aGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACAt              >  1:1951055/1‑93 (MQ=255)

                                                                           aGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACAt              >  2:4380277/1‑93 (MQ=255)

                                                                            gACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACAtt             >  2:628199/1‑93 (MQ=255)

                                                                            gACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACAtt             <  1:4441489/93‑1 (MQ=255)

                                                                            gACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACAtt             >  1:4608914/1‑93 (MQ=255)

                                                                             aCTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTg            >  1:4027874/1‑93 (MQ=255)

                                                                             aCTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTg            <  2:1458985/93‑1 (MQ=255)

                                                                               ttCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCa          <  2:2262701/93‑1 (MQ=255)

                                                                                  aGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCt       <  1:2438615/93‑1 (MQ=255)

                                                                                  aGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCt       >  2:5794333/1‑93 (MQ=255)

                                                                                  aGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCt       <  2:353710/93‑1 (MQ=255)

                                                                                  aGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCt       <  1:1943948/93‑1 (MQ=255)

                                                                                   gCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCtt      >  1:3520857/1‑93 (MQ=255)

                                                                                   gCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCtt      >  2:3251769/1‑93 (MQ=255)

                                                                                   gCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCtt      >  2:1660659/1‑93 (MQ=255)

                                                                                     cccTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTg    <  2:2831908/93‑1 (MQ=255)

                                                                                      ccTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGt   >  2:679777/1‑93 (MQ=255)

                                                                                      ccTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGt   >  2:2437691/1‑93 (MQ=255)

                                                                                       cTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTa  >  1:2424590/1‑93 (MQ=255)

                                                                                       cTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTa  <  2:6485327/93‑1 (MQ=255)

GCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTA  >  NC_000913/1799479‑1799658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTT  >  NC_000913/1799476‑1799661

CAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGA                                                                                              >  SRR4302536.212273/1‑94 (MQ=60)

 AGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGGTCGCGATAAGACTTCAGCACCTGAT                                                                                             >  SRR4302536.1336124/1‑94 (MQ=60)

  GGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATT                                                                                            <  SRR4302536.4373124/94‑1 (MQ=60)

  GGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATT                                                                                            >  SRR4302536.4447305/1‑94 (MQ=60)

    CCATGAAACGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGA                                                                                          <  SRR4302536.4218785/94‑1 (MQ=60)

       TGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAA                                                                                       <  SRR4302536.2472374/94‑1 (MQ=60)

        GCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAAT                                                                                      <  SRR4302536.4437992/94‑1 (MQ=60)

          AGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTT                                                                                    <  SRR4302536.3756031/94‑1 (MQ=60)

            CGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGT                                                                                  <  SRR4302536.1968100/94‑1 (MQ=60)

             GAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTA                                                                                 >  SRR4302536.4594304/1‑94 (MQ=60)

              AACGTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTAC                                                                                <  SRR4302536.5798189/94‑1 (MQ=60)

              AACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTAC                                                                                >  SRR4302536.5155945/1‑94 (MQ=60)

              AACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTAC                                                                                >  SRR4302536.4998004/1‑94 (MQ=60)

              AACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTAC                                                                                <  SRR4302536.1604971/94‑1 (MQ=60)

               ACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACG                                                                               <  SRR4302536.708688/94‑1 (MQ=60)

               ACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACG                                                                               <  SRR4302536.2231319/94‑1 (MQ=60)

                CCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGT                                                                              <  SRR4302536.5646415/94‑1 (MQ=60)

                CCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGT                                                                              <  SRR4302536.347126/94‑1 (MQ=60)

                  TGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGA                                                                            >  SRR4302536.489959/1‑94 (MQ=60)

                   GACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGAC                                                                           >  SRR4302536.2082730/1‑94 (MQ=60)

                   GACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGAC                                                                           >  SRR4302536.1155183/1‑94 (MQ=60)

                    ACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACC                                                                          <  SRR4302536.12774/94‑1 (MQ=60)

                     CGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCC                                                                         <  SRR4302536.3160766/94‑1 (MQ=60)

                      GGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCG                                                                        <  SRR4302536.1212077/94‑1 (MQ=60)

                       gccccgtcCGGTGGCAGCTACCAAACTCGGCCATACGGAGCGGCAGATCGCGATAAAACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGG                                                                       <  SRR4302536.3252427/86‑1 (MQ=60)

                        CTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGG                                                                      >  SRR4302536.5112959/1‑94 (MQ=60)

                          CGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCA                                                                    <  SRR4302536.2680663/94‑1 (MQ=60)

                          CGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGAATTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCA                                                                    >  SRR4302536.737876/1‑94 (MQ=60)

                           GTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAG                                                                   >  SRR4302536.6464630/1‑94 (MQ=60)

                           GTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAG                                                                   <  SRR4302536.2356318/94‑1 (MQ=60)

                            TTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGT                                                                  >  SRR4302536.311643/1‑94 (MQ=60)

                             TACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTT                                                                 <  SRR4302536.6468665/94‑1 (MQ=60)

                              ACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTC                                                                <  SRR4302536.3556148/94‑1 (MQ=60)

                               CGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCA                                                               <  SRR4302536.3742289/94‑1 (MQ=60)

                               CGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCA                                                               <  SRR4302536.4560662/94‑1 (MQ=60)

                                GGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCAT                                                              >  SRR4302536.5382638/1‑94 (MQ=60)

                                GGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCAT                                                              >  SRR4302536.1335862/1‑94 (MQ=60)

                                  TGGCAGCTACCAATCTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGCTCATCG                                                            <  SRR4302536.4346958/94‑1 (MQ=60)

                                  TGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCG                                                            <  SRR4302536.389910/94‑1 (MQ=60)

                                  TGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCG                                                            >  SRR4302536.4111044/1‑94 (MQ=60)

                                   GGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGG                                                           >  SRR4302536.2914383/1‑94 (MQ=60)

                                    GCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGC                                                          <  SRR4302536.527699/94‑1 (MQ=60)

                                    GCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGC                                                          <  SRR4302536.3369998/94‑1 (MQ=60)

                                      AGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTT                                                        >  SRR4302536.2638311/1‑94 (MQ=60)

                                      AGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTT                                                        <  SRR4302536.2755393/94‑1 (MQ=60)

                                         TACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAAT                                                     <  SRR4302536.1691341/94‑1 (MQ=60)

                                          ACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATG                                                    <  SRR4302536.278336/94‑1 (MQ=60)

                                            CAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCA                                                  <  SRR4302536.2505363/94‑1 (MQ=60)

                                             AAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAG                                                 <  SRR4302536.5991145/94‑1 (MQ=60)

                                             AAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAG                                                 <  SRR4302536.3195488/94‑1 (MQ=60)

                                             AAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGGTCATCAGCTTAATGCAG                                                 >  SRR4302536.1466489/1‑94 (MQ=60)

                                              AACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGT                                                <  SRR4302536.767356/94‑1 (MQ=60)

                                              AACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGT                                                <  SRR4302536.789716/94‑1 (MQ=60)

                                                CTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTAT                                              <  SRR4302536.4781664/94‑1 (MQ=60)

                                                 GCGGCCATACGCAGCGCCAGATCGCGATAAGACTTCAGCCTCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATT                                             <  SRR4302536.169701/94‑1 (MQ=60)

                                                   GGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCA                                           >  SRR4302536.5166350/1‑94 (MQ=60)

                                                   GGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCA                                           >  SRR4302536.2036801/1‑94 (MQ=60)

                                                    GCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCAC                                          <  SRR4302536.6230790/94‑1 (MQ=60)

                                                     CCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACG                                         >  SRR4302536.1627873/1‑94 (MQ=60)

                                                      CATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGG                                        >  SRR4302536.2004957/1‑94 (MQ=60)

                                                      CATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGG                                        >  SRR4302536.743161/1‑94 (MQ=60)

                                                      CATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGG                                        <  SRR4302536.1894058/94‑1 (MQ=60)

                                                       ATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGT                                       >  SRR4302536.4442271/1‑94 (MQ=60)

                                                       ATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGT                                       >  SRR4302536.6024441/1‑94 (MQ=60)

                                                       ATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGT                                       <  SRR4302536.1150566/94‑1 (MQ=60)

                                                        TACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTT                                      <  SRR4302536.5356940/94‑1 (MQ=60)

                                                         ACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTC                                     <  SRR4302536.495081/94‑1 (MQ=60)

                                                         ACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTC                                     >  SRR4302536.926125/1‑94 (MQ=60)

                                                          CGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCT                                    <  SRR4302536.2167771/94‑1 (MQ=60)

                                                          CGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCT                                    <  SRR4302536.2585571/94‑1 (MQ=60)

                                                           GCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTC                                   <  SRR4302536.5211005/94‑1 (MQ=60)

                                                            CAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCA                                  <  SRR4302536.4421235/94‑1 (MQ=60)

                                                            CAGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCA                                  <  SRR4302536.1110518/94‑1 (MQ=60)

                                                             AGCGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAG                                 <  SRR4302536.3329478/94‑1 (MQ=60)

                                                               CGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAA                               <  SRR4302536.3515186/94‑1 (MQ=60)

                                                               CGGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAA                               <  SRR4302536.281253/94‑1 (MQ=60)

                                                                GGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAG                              <  SRR4302536.5301006/94‑1 (MQ=60)

                                                                GGCAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAG                              >  SRR4302536.4224563/1‑94 (MQ=60)

                                                                  CAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAT                            <  SRR4302536.6092468/94‑1 (MQ=60)

                                                                  CAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAT                            <  SRR4302536.1494666/94‑1 (MQ=60)

                                                                  CAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAT                            <  SRR4302536.6323607/94‑1 (MQ=60)

                                                                  CAGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGAT                            >  SRR4302536.5880050/1‑94 (MQ=60)

                                                                   AGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATG                           <  SRR4302536.6209309/94‑1 (MQ=60)

                                                                   AGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATG                           >  SRR4302536.458854/1‑94 (MQ=60)

                                                                   AGATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATG                           >  SRR4302536.1090023/1‑94 (MQ=60)

                                                                    GATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCAGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGT                          >  SRR4302536.3289007/1‑94 (MQ=60)

                                                                     ATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTG                         >  SRR4302536.1060426/1‑94 (MQ=60)

                                                                     ATCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTG                         <  SRR4302536.2375624/94‑1 (MQ=60)

                                                                      TCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGG                        <  SRR4302536.6193521/94‑1 (MQ=60)

                                                                      TCGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGG                        <  SRR4302536.6440244/94‑1 (MQ=60)

                                                                       CGCGATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGT                       <  SRR4302536.5377895/94‑1 (MQ=60)

                                                                           ATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAAC                   >  SRR4302536.6292891/1‑94 (MQ=60)

                                                                           ATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAAC                   >  SRR4302536.6390227/1‑94 (MQ=60)

                                                                           ATAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAAC                   >  SRR4302536.4170705/1‑94 (MQ=60)

                                                                            TAAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACA                  >  SRR4302536.1007752/1‑94 (MQ=60)

                                                                             AAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACAT                 >  SRR4302536.2009731/1‑94 (MQ=60)

                                                                             AAGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACAT                 >  SRR4302536.3087775/1‑94 (MQ=60)

                                                                              AGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATT                <  SRR4302536.4647906/94‑1 (MQ=60)

                                                                              AGACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATT                >  SRR4302536.4747354/1‑94 (MQ=60)

                                                                               GACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTG               <  SRR4302536.4574830/94‑1 (MQ=60)

                                                                               GACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTG               >  SRR4302536.4147339/1‑94 (MQ=60)

                                                                               GACTTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTG               <  SRR4302536.5179828/94‑1 (MQ=60)

                                                                                  TTCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCAT            <  SRR4302536.129570/94‑1 (MQ=60)

                                                                                   TCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATC           <  SRR4302536.4412031/94‑1 (MQ=60)

                                                                                   TCAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATC           <  SRR4302536.2218519/94‑1 (MQ=60)

                                                                                    CAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCT          <  SRR4302536.4569335/94‑1 (MQ=60)

                                                                                    CAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCT          <  SRR4302536.4645577/94‑1 (MQ=60)

                                                                                    CAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCT          >  SRR4302536.5078204/1‑94 (MQ=60)

                                                                                    CAGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGGTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCT          >  SRR4302536.2800458/1‑94 (MQ=60)

                                                                                     AGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTT         <  SRR4302536.2512330/94‑1 (MQ=60)

                                                                                     AGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTT         <  SRR4302536.3607686/94‑1 (MQ=60)

                                                                                     AGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTT         >  SRR4302536.3626052/1‑94 (MQ=60)

                                                                                     AGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTT         <  SRR4302536.5424210/94‑1 (MQ=60)

                                                                                     AGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTT         <  SRR4302536.2002396/94‑1 (MQ=60)

                                                                                     AGCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATGGCATCTT         <  SRR4302536.2265619/94‑1 (MQ=60)

                                                                                      GCCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTT        <  SRR4302536.5032069/94‑1 (MQ=60)

                                                                                       CCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTG       <  SRR4302536.1405857/94‑1 (MQ=60)

                                                                                       CCCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTG       <  SRR4302536.1266660/94‑1 (MQ=60)

                                                                                        CCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGT      >  SRR4302536.5668969/1‑94 (MQ=60)

                                                                                        CCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGT      >  SRR4302536.5482791/1‑94 (MQ=60)

                                                                                        CCCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGT      <  SRR4302536.6901200/94‑1 (MQ=60)

                                                                                         CCTGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTA     >  SRR4302536.2497885/1‑94 (MQ=60)

                                                                                           TGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGT   >  SRR4302536.6417577/1‑94 (MQ=60)

                                                                                           TGATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGT   >  SRR4302536.6811329/1‑94 (MQ=60)

                                                                                            GATTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTT  >  SRR4302536.4877190/1‑94 (MQ=60)

CAGGCCATGCAGCGAACCTGACGGCTCGTTACGGTGGCAGCTACCAAACTCGGCCATACGCAGCGGCAGATCGCGATAAGACTTCAGCCCCTGGTTGAAAATTTGTACGTGACCCGGGCAGTTCATCGGCTTAATGCAGTATTCACGGTTCTCAGAAGATGTGGTGAACATTGCATCTTTGTAGTT  >  NC_000913/1799476‑1799661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`