Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,908,347	T→C	G16G (GGT→GGC)	b1828 →	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,908,347	0	T	C	98.2%	511.7 / ‑1.2	166	G16G (GGT→GGC)	b1828	putative transport protein
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (89/74); total (91/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 179 total aligned reads displayed.

AATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACC  >  NC_000913/1908257‑1908435

aaTCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGgcgc                                                                                        >  1:3983535/1‑93 (MQ=255)

  tCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGa                                                                                      >  2:4414173/1‑93 (MQ=255)

     tACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGatat                                                                                   <  1:3450841/93‑1 (MQ=255)

     tACCCCTGTTGATTATTCAACAAAGATATAAAATTCCTATGCCAAAAGTTCAGGACGACGGCCTGCCATTGCCCCAGCGATACGGCGCGatat                                                                                   >  1:2370970/1‑93 (MQ=255)

      aCCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATAtt                                                                                  >  2:3125093/1‑93 (MQ=255)

       ccccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTa                                                                                 >  1:162632/1‑93 (MQ=255)

        cccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTaa                                                                                <  2:745112/93‑1 (MQ=255)

        cccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTaa                                                                                <  1:2228470/93‑1 (MQ=255)

         ccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTGAGGCAGAGGGCATGCAATTTCCCCAGCGAAACGGCGCGATATAAAc                                                                               >  1:1274192/1‑93 (MQ=255)

           tGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATCTTAACCa                                                                             <  2:2882624/93‑1 (MQ=255)

           tGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCa                                                                             >  2:2404838/1‑93 (MQ=255)

           tGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCa                                                                             >  2:3963685/1‑93 (MQ=255)

            gTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAt                                                                            >  2:1860112/1‑93 (MQ=255)

            gTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAt                                                                            <  1:2760379/93‑1 (MQ=255)

            gTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAt                                                                            >  1:2555267/1‑93 (MQ=255)

             ttGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAtt                                                                           >  2:1573329/1‑93 (MQ=255)

               gATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATtgt                                                                         >  2:4069157/1‑93 (MQ=255)

               gATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATtgt                                                                         <  1:258240/93‑1 (MQ=255)

               gATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATtgt                                                                         <  1:669241/93‑1 (MQ=255)

                  tattCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGAt                                                                      >  2:1438909/1‑93 (MQ=255)

                   attCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGAtt                                                                     <  1:1965122/93‑1 (MQ=255)

                   attCACCAAAGATATAAAATT‑CTATTCCAAAAGTTCAAGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGGTATTTACCCTTGTGATTg                                                                    >  1:3108971/1‑93 (MQ=255)

                    ttCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTg                                                                    <  1:1794197/93‑1 (MQ=255)

                    ttCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTg                                                                    <  2:1318016/93‑1 (MQ=255)

                     tCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTgg                                                                   <  1:3925690/93‑1 (MQ=255)

                      cACCAAAGATATAAAATTCCTATGCCAAAAGTTCGGGCCGACGTCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGt                                                                  <  2:1049274/93‑1 (MQ=255)

                      cACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGt                                                                  <  2:184471/93‑1 (MQ=255)

                      cACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGt                                                                  >  2:2163293/1‑93 (MQ=255)

                      cACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGt                                                                  >  1:3275525/1‑93 (MQ=255)

                       aCCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTa                                                                 <  2:1939938/93‑1 (MQ=255)

                       aCCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTa                                                                 >  1:4343914/1‑93 (MQ=255)

                        ccAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAt                                                                >  2:3148686/1‑93 (MQ=255)

                         cAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGTCGCGATATTAACCATTGTGATTGGTAtt                                                               >  2:4604927/1‑93 (MQ=255)

                         cAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAtt                                                               >  2:3841243/1‑93 (MQ=255)

                          aaaGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAttt                                                              >  1:3492496/1‑93 (MQ=255)

                          aaaGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAttt                                                              >  2:646359/1‑93 (MQ=255)

                          aaaGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAttt                                                              <  1:4601888/93‑1 (MQ=255)

                          aaaGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAttt                                                              >  1:4469295/1‑93 (MQ=255)

                          aaaGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAttt                                                              >  1:2849019/1‑93 (MQ=255)

                          aaaGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAttt                                                              >  1:1742537/1‑93 (MQ=255)

                           aaGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTc                                                             <  2:4687334/93‑1 (MQ=255)

                             gATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGa                                                           <  2:2319005/93‑1 (MQ=255)

                             gATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGa                                                           <  1:4548586/93‑1 (MQ=255)

                              atatAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGAt                                                          <  1:3322423/93‑1 (MQ=255)

                              atatAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGAt                                                          <  2:368075/93‑1 (MQ=255)

                               tataAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATg                                                         <  2:4393868/93‑1 (MQ=255)

                                ataAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATgg                                                        >  1:3685994/1‑93 (MQ=255)

                                 taAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTCACCATTGTGATTGGTATTTGGTTGGc                                                       >  1:3573866/1‑93 (MQ=255)

                                 taAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGc                                                       >  1:3573867/1‑93 (MQ=255)

                                 taAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGc                                                       >  2:2243335/1‑93 (MQ=255)

                                  aaaaTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGcc                                                      >  1:2966780/1‑93 (MQ=255)

                                  aaaaTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGcc                                                      <  2:1066061/93‑1 (MQ=255)

                                   aaaTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCg                                                     >  1:1439484/1‑93 (MQ=255)

                                     aTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTc                                                   <  1:411330/93‑1 (MQ=255)

                                     aTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTc                                                   <  1:4179199/93‑1 (MQ=255)

                                       tCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCt                                                 >  1:2932946/1‑93 (MQ=255)

                                        tcTATGCCAAAAGTTCAGTCAGACGGCCTGCCAGTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCtt                                                <  2:1145834/92‑1 (MQ=255)

                                        ccTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCtt                                                >  1:1954265/1‑93 (MQ=255)

                                         cTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTg                                               >  2:2031603/1‑93 (MQ=255)

                                          ctgcccAAAAGTTCAGGCCCACGGCCTTCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGCGATTGGTATTACGATGGCCGTCCTTGa                                              <  1:1012095/89‑1 (MQ=255)

                                            tGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGCCg                                            >  2:1511062/1‑93 (MQ=255)

                                            tGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACg                                            >  2:1511066/1‑93 (MQ=255)

                                            tGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACg                                            >  1:100263/1‑93 (MQ=255)

                                                aaaaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGCCGgcgc                                        >  2:4276625/1‑93 (MQ=255)

                                                aaaaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGgcgc                                        >  2:4720209/1‑93 (MQ=255)

                                                aaaaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGgcgc                                        >  2:2491694/1‑93 (MQ=255)

                                                 aaaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCa                                       >  2:1014262/1‑93 (MQ=255)

                                                  aaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGTCGCGATATTAACCATTGTGATTGGCATTTCGATGGGCGTCCTTGACGGCGCaa                                      >  2:518183/1‑93 (MQ=255)

                                                  aaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCaa                                      <  1:761152/93‑1 (MQ=255)

                                                  aaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCaa                                      <  1:559806/93‑1 (MQ=255)

                                                   aGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAAt                                     <  1:579154/93‑1 (MQ=255)

                                                   aGTCCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAAt                                     <  2:2241762/93‑1 (MQ=255)

                                                       cAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGcc                                 <  2:3199664/93‑1 (MQ=255)

                                                         ggCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCaa                               <  1:43391/93‑1 (MQ=255)

                                                         ggCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCaa                               <  2:2908301/93‑1 (MQ=255)

                                                          gCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAAc                              <  1:618921/93‑1 (MQ=255)

                                                           ccGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACg                             <  1:3493550/93‑1 (MQ=255)

                                                             gACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTc                           <  2:3002378/93‑1 (MQ=255)

                                                             caCGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATGTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTc                           <  1:2249285/92‑1 (MQ=255)

                                                              aCGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCg                          >  2:1648921/1‑93 (MQ=255)

                                                                ggCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGcc                        >  1:1598965/1‑93 (MQ=255)

                                                                 gCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGccc                       >  1:3235750/1‑93 (MQ=255)

                                                                 gCCTGCCATTGCCCCAGCGATACGGCGCCATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGccc                       <  1:1657557/93‑1 (MQ=255)

                                                                   cTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTg                     >  2:4367875/1‑93 (MQ=255)

                                                                   cTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTg                     >  1:2618393/1‑93 (MQ=255)

                                                                   cTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTg                     <  2:4629565/93‑1 (MQ=255)

                                                                    tGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGc                    >  1:3911617/1‑93 (MQ=255)

                                                                       cATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCcaa                 >  1:209006/1‑93 (MQ=255)

                                                                        aTTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCcaac                >  2:1746288/1‑93 (MQ=255)

                                                                         ttGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCcaaca               <  1:1418411/93‑1 (MQ=255)

                                                                            ccccAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATc            <  2:2014022/93‑1 (MQ=255)

                                                                              ccAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGc          >  2:3926308/1‑93 (MQ=255)

                                                                                 gCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCAc       >  1:387869/1‑93 (MQ=255)

                                                                                 gCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCAc       >  2:3548250/1‑93 (MQ=255)

                                                                                   gATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACgg     >  2:2101366/1‑93 (MQ=255)

                                                                                   gATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACCGCGCAATCGCCAACGGCGCCCTGCCAACAATCGCCACgg     >  2:2609114/1‑93 (MQ=255)

                                                                                    aTACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGa    <  2:4018543/93‑1 (MQ=255)

                                                                                    aTACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGa    >  1:2288702/1‑93 (MQ=255)

                                                                                     tACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGAc   <  1:2341855/93‑1 (MQ=255)

                                                                                      aCGGCGCGATATCAACCATTGTGCTTGGTATTACGATGGCCGTCATTGACGGCGCAATCGCCACCGTCGCCCTGCCAACAATCGCCACGGAcc  <  1:2186195/93‑1 (MQ=255)

AATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACC  >  NC_000913/1908257‑1908435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCT  >  NC_000913/1908255‑1908436

AGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCG                                                                                          <  SRR4302544.928642/94‑1 (MQ=60)

AGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCG                                                                                          <  SRR4302544.504311/94‑1 (MQ=60)

 GAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGC                                                                                         >  SRR4302544.4023182/1‑94 (MQ=60)

 GAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGC                                                                                         <  SRR4302544.1585482/94‑1 (MQ=60)

   ATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGA                                                                                       <  SRR4302544.2201645/94‑1 (MQ=60)

    TCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGAT                                                                                      <  SRR4302544.2634032/94‑1 (MQ=60)

     CATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATA                                                                                     >  SRR4302544.467105/1‑94 (MQ=60)

      ATACCCCTGTTGATTATTCAACAAAGATATAAAATTCCTATGCCAAAAGTTCAGGACGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATAT                                                                                    >  SRR4302544.2393406/1‑94 (MQ=60)

       TACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATT                                                                                   <  SRR4302544.3483795/94‑1 (MQ=60)

       TACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATT                                                                                   >  SRR4302544.268841/1‑94 (MQ=60)

        ACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTA                                                                                  >  SRR4302544.164221/1‑94 (MQ=60)

         CCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAA                                                                                 >  SRR4302544.375066/1‑94 (MQ=60)

          CCCTGTTTATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAAC                                                                                <  SRR4302544.3231368/94‑1 (MQ=60)

          CCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTGAGGCAGAGGGCATGCAATTTCCCCAGCGAAACGGCGCGATATAAAC                                                                                >  SRR4302544.1286737/1‑94 (MQ=55)

          CCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAAC                                                                                <  SRR4302544.2249439/94‑1 (MQ=60)

             TGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAT                                                                             <  SRR4302544.3291340/94‑1 (MQ=60)

             TGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAT                                                                             >  SRR4302544.2579800/1‑94 (MQ=60)

              GTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATT                                                                            <  SRR4302544.634304/94‑1 (MQ=60)

              GTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATT                                                                            <  SRR4302544.2786940/94‑1 (MQ=60)

                 GATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTG                                                                         <  SRR4302544.990450/94‑1 (MQ=60)

                 GATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTG                                                                         <  SRR4302544.260975/94‑1 (MQ=60)

                 GATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTG                                                                         >  SRR4302544.700727/1‑94 (MQ=60)

                 GATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTG                                                                         <  SRR4302544.675848/94‑1 (MQ=60)

                 GATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTG                                                                         <  SRR4302544.1626733/94‑1 (MQ=60)

                    TATTCACCAAAGATATAAAATT‑CTATTCCAAAAGTTCAAGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGGTATTTACCCTTGTGATTG                                                                     >  SRR4302544.3138558/1‑94 (MQ=60)

                     ATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTG                                                                     <  SRR4302544.1984202/94‑1 (MQ=60)

                     ATTCACCAAAGATATAAAATTCCTATGCCAAAAGTGCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTG                                                                     <  SRR4302544.1984201/94‑1 (MQ=60)

                      TTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGG                                                                    <  SRR4302544.1811742/94‑1 (MQ=60)

                       TCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGT                                                                   <  SRR4302544.3964683/94‑1 (MQ=60)

                       TCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGT                                                                   >  SRR4302544.3306750/1‑94 (MQ=60)

                       TCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGT                                                                   >  SRR4302544.282220/1‑94 (MQ=60)

                        CACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTA                                                                  >  SRR4302544.4388087/1‑94 (MQ=60)

                         ACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAT                                                                 <  SRR4302544.1424524/94‑1 (MQ=60)

                           CAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTT                                                               >  SRR4302544.2876539/1‑94 (MQ=60)

                           CAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTT                                                               >  SRR4302544.1759597/1‑94 (MQ=60)

                           CAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTT                                                               >  SRR4302544.4514245/1‑94 (MQ=60)

                           CAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTT                                                               >  SRR4302544.1670560/1‑94 (MQ=60)

                           CAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTT                                                               >  SRR4302544.3525795/1‑94 (MQ=60)

                            AAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTC                                                              <  SRR4302544.4647633/94‑1 (MQ=60)

                            AAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTC                                                              <  SRR4302544.4347547/94‑1 (MQ=60)

                             AAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGCCCTGCCATTGCGCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCG                                                             <  SRR4302544.2431853/94‑1 (MQ=60)

                               GATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGAT                                                           <  SRR4302544.4594009/94‑1 (MQ=60)

                                ATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATG                                                          <  SRR4302544.1772987/94‑1 (MQ=60)

                                ATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATG                                                          <  SRR4302544.3354071/94‑1 (MQ=60)

                                 TATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGG                                                         >  SRR4302544.3722741/1‑94 (MQ=60)

                                  ATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTCACCATTGTGATTGGTATTTGGTTGGC                                                        >  SRR4302544.3608140/1‑94 (MQ=60)

                                  ATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGC                                                        >  SRR4302544.3608141/1‑94 (MQ=60)

                                  ATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTAGATGGC                                                        >  SRR4302544.3053228/1‑94 (MQ=60)

                                   TAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCC                                                       >  SRR4302544.2995244/1‑94 (MQ=60)

                                   TAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCC                                                       >  SRR4302544.1447433/1‑94 (MQ=60)

                                    AAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCG                                                      >  SRR4302544.1453813/1‑94 (MQ=60)

                                       ATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCC                                                   <  SRR4302544.4220592/94‑1 (MQ=60)

                                       ATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCC                                                   <  SRR4302544.415525/94‑1 (MQ=60)

                                        TTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCT                                                  >  SRR4302544.2961129/1‑94 (MQ=60)

                                        TTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCT                                                  >  SRR4302544.2238259/1‑94 (MQ=60)

                                         TCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTT                                                 >  SRR4302544.1973222/1‑94 (MQ=60)

                                         TCCTATGCCAAAAGTTCAGGCCGACGGCCTACCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTT                                                 <  SRR4302544.1272964/94‑1 (MQ=60)

                                          CCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTG                                                >  SRR4302544.1735311/1‑94 (MQ=60)

                                            ctgcCCAAAAGTTCAGGCCCACGGCCTTCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGCGATTGGTATTACGATGGCCGTCCTTGAC                                              <  SRR4302544.1022181/90‑1 (MQ=60)

                                            TATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGAC                                              <  SRR4302544.2303095/94‑1 (MQ=60)

                                             ATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGCTTGGTATTTCGATGGCCGTCCTTGACG                                             >  SRR4302544.403173/1‑94 (MQ=60)

                                             ATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACG                                             >  SRR4302544.101183/1‑94 (MQ=60)

                                                CCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCG                                          >  SRR4302544.2977777/1‑94 (MQ=60)

                                                CCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCG                                          <  SRR4302544.2028429/94‑1 (MQ=60)

                                                   AAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAA                                       >  SRR4302544.3501336/1‑94 (MQ=60)

                                                    AAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAAT                                      <  SRR4302544.565276/94‑1 (MQ=60)

                                                    AAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAAT                                      <  SRR4302544.768574/94‑1 (MQ=60)

                                                     AGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATC                                     <  SRR4302544.584812/94‑1 (MQ=60)

                                                     AGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATC                                     >  SRR4302544.3386612/1‑94 (MQ=60)

                                                       TTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGC                                   >  SRR4302544.3786477/1‑94 (MQ=60)

                                                         CAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCA                                 >  SRR4302544.3960945/1‑94 (MQ=60)

                                                          AGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAA                                >  SRR4302544.2487918/1‑94 (MQ=60)

                                                           GGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAAC                               <  SRR4302544.43818/94‑1 (MQ=60)

                                                            GCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACG                              <  SRR4302544.624997/94‑1 (MQ=60)

                                                            GCCGACGGCCTGCCATTGCCCCAGCGATAAGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCAGTCCTTGACGGCGCAATAGCCAACG                              >  SRR4302544.4419286/1‑94 (MQ=60)

                                                             CCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGT                             <  SRR4302544.3526861/94‑1 (MQ=60)

                                                              CGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTC                            >  SRR4302544.4582397/1‑94 (MQ=60)

                                                               CACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATGTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCG                           <  SRR4302544.2270423/94‑1 (MQ=60)

                                                                 CGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCC                         >  SRR4302544.3582230/1‑94 (MQ=60)

                                                                 CGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCC                         >  SRR4302544.1614726/1‑94 (MQ=60)

                                                                  GGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCC                        >  SRR4302544.3266723/1‑94 (MQ=60)

                                                                   GCCTGCCATTGCCCCAGCGATACGGCGCCATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCT                       <  SRR4302544.1673891/94‑1 (MQ=60)

                                                                    CCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTG                      >  SRR4302544.2643523/1‑94 (MQ=60)

                                                                     CTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGC                     >  SRR4302544.3950475/1‑94 (MQ=60)

                                                                     CCGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGACGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGC                     >  SRR4302544.4036245/1‑94 (MQ=60)

                                                                       GCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCA                   <  SRR4302544.3929004/94‑1 (MQ=60)

                                                                        CCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAA                  >  SRR4302544.211156/1‑94 (MQ=60)

                                                                           TTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAA               <  SRR4302544.1432532/94‑1 (MQ=60)

                                                                              CCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCG            >  SRR4302544.234525/1‑94 (MQ=60)

                                                                              CCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCG            >  SRR4302544.4618322/1‑94 (MQ=60)

                                                                               CCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGC           >  SRR4302544.4196859/1‑94 (MQ=60)

                                                                                CCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCC          >  SRR4302544.62027/1‑94 (MQ=60)

                                                                                  CGCGATCCGGCGCGATATTAACCATTGTGGTTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCAC        <  SRR4302544.3643344/94‑1 (MQ=60)

                                                                                  AGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCAC        >  SRR4302544.391811/1‑94 (MQ=60)

                                                                                    CGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCCACGTCGCCCTGCCAACAATCGCCACGG      >  SRR4302544.2381301/1‑94 (MQ=60)

                                                                                     GATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGA     >  SRR4302544.2310276/1‑94 (MQ=60)

                                                                                       TACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACC   <  SRR4302544.2364040/94‑1 (MQ=60)

                                                                                       TACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACC   <  SRR4302544.3525823/94‑1 (MQ=60)

                                                                                        ACGGCGCGATATCAACCATTGTGCTTGGTATTACGATGGCCGTCATTGACGGCGCAATCGCCACCGTCGCCCTGCCAACAATCGCCACGGACCT  <  SRR4302544.2206867/94‑1 (MQ=60)

AGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCT  >  NC_000913/1908255‑1908436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`