Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,908,347 T→C G16G (GGT→GGC)  b1828 → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,908,3470TC99.6% 760.5 / ‑5.6 238G16G (GGT→GGC) b1828putative transport protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (138/99);  total (139/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 245 total aligned reads displayed.

AATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCT  >  NC_000913/1908257‑1908436
                                                                                          |                                                                                         
aaTCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGgcgc                                                                                         >  1:1695909/1‑93 (MQ=255)
 aTCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGcgcg                                                                                        <  2:1555591/93‑1 (MQ=255)
   cATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGat                                                                                      <  2:484636/93‑1 (MQ=255)
     tACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGatat                                                                                    <  2:619059/93‑1 (MQ=255)
     tACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGatat                                                                                    <  2:427851/93‑1 (MQ=255)
     tACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGatat                                                                                    >  1:1878884/1‑93 (MQ=255)
     tACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGatat                                                                                    >  2:215435/1‑93 (MQ=255)
     tACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGatat                                                                                    <  2:1179187/93‑1 (MQ=255)
     tACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGAGATACGGCGCGatat                                                                                    >  2:5163486/1‑93 (MQ=255)
      aCCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATAtt                                                                                   >  2:4968605/1‑93 (MQ=255)
       ccccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTa                                                                                  <  2:4197870/93‑1 (MQ=255)
       ccccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTa                                                                                  >  1:3194720/1‑93 (MQ=255)
       ccccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTa                                                                                  >  1:889001/1‑93 (MQ=255)
         ccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAAc                                                                                >  2:834385/1‑93 (MQ=255)
         ccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAAc                                                                                <  1:4503244/93‑1 (MQ=255)
         ccTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAAc                                                                                <  2:2628273/93‑1 (MQ=255)
          cTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAAcc                                                                               >  1:3758991/1‑93 (MQ=255)
           tGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCa                                                                              >  1:5546851/1‑93 (MQ=255)
            gTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAt                                                                             >  1:5482840/1‑93 (MQ=255)
             ttGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAtt                                                                            >  1:2774989/1‑93 (MQ=255)
             ttGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAtt                                                                            <  1:5258880/93‑1 (MQ=255)
              tGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATtg                                                                           <  2:2361312/93‑1 (MQ=255)
               gATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATtgt                                                                          <  1:6114137/93‑1 (MQ=255)
               gATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATtgt                                                                          >  1:937929/1‑93 (MQ=255)
                  tattCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGAt                                                                       >  2:4600872/1‑93 (MQ=255)
                  tattCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGAt                                                                       <  2:5154793/93‑1 (MQ=255)
                   tttCACCAAAGATATAAAATGCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGAtt                                                                      <  1:6501404/92‑1 (MQ=255)
                   attCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGAtt                                                                      <  2:792965/93‑1 (MQ=255)
                   attCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGAtt                                                                      <  2:1796987/93‑1 (MQ=255)
                   attCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGAtt                                                                      <  1:5013713/93‑1 (MQ=255)
                    ttCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTg                                                                     >  1:1644836/1‑93 (MQ=255)
                    ttCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTCCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTg                                                                     <  1:3985540/93‑1 (MQ=255)
                     tCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTgg                                                                    >  1:2951495/1‑93 (MQ=255)
                      cACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGt                                                                   <  2:1008394/93‑1 (MQ=255)
                      cACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGt                                                                   <  1:4898007/93‑1 (MQ=255)
                        ccAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAt                                                                 <  1:2155065/93‑1 (MQ=255)
                        ccAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAt                                                                 >  2:3657294/1‑93 (MQ=255)
                         cAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAtt                                                                <  2:138694/93‑1 (MQ=255)
                         cAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAtt                                                                >  1:5889964/1‑93 (MQ=255)
                           aaGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTc                                                              <  1:4474357/93‑1 (MQ=255)
                            aGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCg                                                             >  1:1377228/1‑93 (MQ=255)
                              atatAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGAt                                                           >  2:5876966/1‑93 (MQ=255)
                              atatAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGAt                                                           <  2:3317472/93‑1 (MQ=255)
                               tataAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATg                                                          <  2:2211753/93‑1 (MQ=255)
                               tataAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATg                                                          >  2:198947/1‑93 (MQ=255)
                                 taAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGc                                                        >  1:575795/1‑93 (MQ=255)
                                 taAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGc                                                        >  1:5414116/1‑93 (MQ=255)
                                   aaaTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCg                                                      >  1:3511708/1‑93 (MQ=255)
                                    aaTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGt                                                     >  1:4844414/1‑93 (MQ=255)
                                    aaTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGt                                                     <  2:5444194/93‑1 (MQ=255)
                                     aTTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTc                                                    >  2:1104609/1‑93 (MQ=255)
                                      ttCTTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTcc                                                   <  1:2356332/93‑1 (MQ=255)
                                      ttCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTcc                                                   <  1:2238106/93‑1 (MQ=255)
                                      ttCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTcc                                                   <  2:4463475/93‑1 (MQ=255)
                                       tCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCt                                                  <  2:2844606/93‑1 (MQ=255)
                                       tCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCt                                                  >  1:6285979/1‑93 (MQ=255)
                                          tATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGa                                               >  2:5396071/1‑93 (MQ=255)
                                          tATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGa                                               >  2:5978451/1‑93 (MQ=255)
                                          tATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGACGTCCTTGa                                               >  1:2854420/1‑93 (MQ=255)
                                           aTGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGAc                                              >  2:3950373/1‑93 (MQ=255)
                                           aTGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGAc                                              >  1:5946298/1‑93 (MQ=255)
                                           aTGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGAc                                              >  1:3216009/1‑93 (MQ=255)
                                            tGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACg                                             >  2:5816337/1‑93 (MQ=255)
                                              ccAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGgc                                           <  1:695088/93‑1 (MQ=255)
                                              ccAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGgc                                           >  2:2110634/1‑93 (MQ=255)
                                               cAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGgcg                                          >  1:1227381/1‑93 (MQ=255)
                                                aaaaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGgcgc                                         >  2:3769057/1‑93 (MQ=255)
                                                 aaaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCa                                        >  1:4064058/1‑93 (MQ=255)
                                                 aaaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCa                                        >  1:3617421/1‑93 (MQ=255)
                                                 aaaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCa                                        >  2:4638609/1‑93 (MQ=255)
                                                 aaaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGACGTCCTTGACGGCGCa                                        >  2:6674146/1‑93 (MQ=255)
                                                  aaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCaa                                       >  2:4949253/1‑93 (MQ=255)
                                                  aaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCaa                                       <  1:4232946/93‑1 (MQ=255)
                                                  aaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCaa                                       <  1:5715155/93‑1 (MQ=255)
                                                   aGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAAt                                      <  2:1397376/93‑1 (MQ=255)
                                                    gTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATc                                     >  2:3073532/1‑93 (MQ=255)
                                                    gTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATc                                     >  1:4589611/1‑93 (MQ=255)
                                                     ttCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCg                                    >  2:6416187/1‑93 (MQ=255)
                                                       cAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGcc                                  <  1:1060415/93‑1 (MQ=255)
                                                          gCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAAc                               <  2:2960268/93‑1 (MQ=255)
                                                          gCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAAc                               <  2:4730458/93‑1 (MQ=255)
                                                           ccGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACg                              <  1:1469058/93‑1 (MQ=255)
                                                           ccGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACg                              <  2:3951732/93‑1 (MQ=255)
                                                              aCGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCg                           >  2:6357507/1‑93 (MQ=255)
                                                              aCGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCg                           >  2:2502820/1‑93 (MQ=255)
                                                                ggCCTGCCATTGCCCCAGCGATACGGCGCGATACTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGcc                         <  2:6546707/93‑1 (MQ=255)
                                                                 gCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGCTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGccc                        <  1:3951756/93‑1 (MQ=255)
                                                                  ccTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCt                       <  1:313332/93‑1 (MQ=255)
                                                                  ccTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCt                       <  1:1803149/93‑1 (MQ=255)
                                                                  ccTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCt                       >  1:1274443/1‑93 (MQ=255)
                                                                  ccTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCt                       >  1:3795905/1‑93 (MQ=255)
                                                                    tGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGc                     >  1:5595343/1‑93 (MQ=255)
                                                                     gCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGcc                    >  2:1917604/1‑93 (MQ=255)
                                                                         ttGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCcaaca                >  1:3026003/1‑93 (MQ=255)
                                                                         ttGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCcaaca                >  1:2208878/1‑93 (MQ=255)
                                                                              ccAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGc           <  1:255825/93‑1 (MQ=255)
                                                                              ccAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGc           >  2:2873844/1‑93 (MQ=255)
                                                                                   gATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACgg      >  2:5671261/1‑93 (MQ=255)
                                                                                     tACGGAGCGATATTAACCATTGTGATTGATATTTCAAAGGACGTCCTTGACGGCGCAATCGCCAACGACGAACTGCCAACAATCGCCAAGGAc    <  1:6725245/93‑1 (MQ=255)
                                                                                       cGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCt  <  2:2414321/93‑1 (MQ=255)
                                                                                          |                                                                                         
AATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCT  >  NC_000913/1908257‑1908436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCAT  >  NC_000913/1908254‑1908440
                                                                                             |                                                                                             
TAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGC                                                                                               >  SRR4302536.1857563/1‑94 (MQ=60)
 AGAGTCATCCCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCAAAAAGTTCAGGCGTACGGCCTGCCATTGCCCCAGCGATACGGCG                                                                                              <  SRR4302536.1774078/94‑1 (MQ=60)
 AGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCG                                                                                              <  SRR4302536.3424802/94‑1 (MQ=60)
 AGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCG                                                                                              >  SRR4302536.3643239/1‑94 (MQ=60)
 AGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCG                                                                                              >  SRR4302536.3679785/1‑94 (MQ=60)
 AGAATCATACCCCTGTTGATTAGTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCG                                                                                              >  SRR4302536.720711/1‑94 (MQ=60)
  GAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGC                                                                                             >  SRR4302536.1745000/1‑94 (MQ=60)
     TCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGAT                                                                                          <  SRR4302536.3109833/94‑1 (MQ=60)
      CATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATA                                                                                         >  SRR4302536.491150/1‑94 (MQ=60)
       ATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATAT                                                                                        >  SRR4302536.1935415/1‑94 (MQ=60)
         ACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTA                                                                                      >  SRR4302536.2941645/1‑94 (MQ=60)
         ACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTA                                                                                      >  SRR4302536.915178/1‑94 (MQ=60)
         ACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTA                                                                                      >  SRR4302536.3288366/1‑94 (MQ=60)
         ACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTA                                                                                      >  SRR4302536.1330569/1‑94 (MQ=60)
         ACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTA                                                                                      <  SRR4302536.1682654/94‑1 (MQ=60)
          CCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAA                                                                                     >  SRR4302536.6681082/1‑94 (MQ=60)
           CCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAAC                                                                                    <  SRR4302536.3256449/94‑1 (MQ=60)
           CCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAAC                                                                                    <  SRR4302536.905233/94‑1 (MQ=60)
            CCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACC                                                                                   <  SRR4302536.4638382/94‑1 (MQ=60)
            CCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACC                                                                                   >  SRR4302536.3871279/1‑94 (MQ=60)
            CCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACC                                                                                   >  SRR4302536.635508/1‑94 (MQ=60)
             CTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCA                                                                                  >  SRR4302536.5712117/1‑94 (MQ=60)
              TGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCAT                                                                                 >  SRR4302536.5646549/1‑94 (MQ=60)
               GTTGATTATTCACTAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATT                                                                                <  SRR4302536.2110777/94‑1 (MQ=60)
               GTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAAGCATT                                                                                >  SRR4302536.2897351/1‑94 (MQ=60)
               GTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATT                                                                                >  SRR4302536.5730588/1‑94 (MQ=60)
               GTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATT                                                                                >  SRR4302536.2857447/1‑94 (MQ=60)
                TTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTG                                                                               <  SRR4302536.5415599/94‑1 (MQ=60)
                TTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTG                                                                               >  SRR4302536.1741668/1‑94 (MQ=60)
                 TGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGT                                                                              >  SRR4302536.1831265/1‑94 (MQ=60)
                 TGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGT                                                                              >  SRR4302536.2993650/1‑94 (MQ=60)
                 TGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGT                                                                              >  SRR4302536.965579/1‑94 (MQ=60)
                  GATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTG                                                                             >  SRR4302536.1194632/1‑94 (MQ=60)
                  GATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTG                                                                             <  SRR4302536.6296370/94‑1 (MQ=60)
                  GATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTG                                                                             <  SRR4302536.78177/94‑1 (MQ=60)
                   ATTATTCAGAAAAGAAATAAAATTCCTAGGCAAAAAGTTCGGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGA                                                                            <  SRR4302536.3302586/94‑1 (MQ=60)
                   ATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGGCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGA                                                                            >  SRR4302536.4047458/1‑94 (MQ=60)
                      TTTCACCAAAGATATAAAATGCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTG                                                                         <  SRR4302536.6695166/94‑1 (MQ=60)
                      ATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTG                                                                         >  SRR4302536.1692673/1‑94 (MQ=60)
                      ATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTG                                                                         <  SRR4302536.5164073/94‑1 (MQ=60)
                       TTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGG                                                                        >  SRR4302536.3038481/1‑94 (MQ=60)
                       TTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTCCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGG                                                                        <  SRR4302536.4103847/94‑1 (MQ=60)
                         CACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTA                                                                      <  SRR4302536.5045313/94‑1 (MQ=60)
                         CACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTA                                                                      >  SRR4302536.2549876/1‑94 (MQ=60)
                          AGCAAAGATATAAAATGCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAT                                                                     <  SRR4302536.2293738/94‑1 (MQ=60)
                          ACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTAT                                                                     <  SRR4302536.6107399/94‑1 (MQ=60)
                           CCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATT                                                                    >  SRR4302536.6065367/1‑94 (MQ=60)
                           CCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATT                                                                    <  SRR4302536.2219833/94‑1 (MQ=60)
                            CAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTT                                                                   >  SRR4302536.5035197/1‑94 (MQ=60)
                            CAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTT                                                                   >  SRR4302536.6880577/1‑94 (MQ=60)
                             AAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTC                                                                  <  SRR4302536.706735/94‑1 (MQ=60)
                             AAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTC                                                                  >  SRR4302536.3217217/1‑94 (MQ=60)
                              AAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCG                                                                 <  SRR4302536.5770011/94‑1 (MQ=60)
                              AAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCG                                                                 >  SRR4302536.1418254/1‑94 (MQ=60)
                              AAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCG                                                                 <  SRR4302536.5939263/94‑1 (MQ=60)
                              AAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCG                                                                 <  SRR4302536.4608766/94‑1 (MQ=60)
                              AAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCG                                                                 <  SRR4302536.576706/94‑1 (MQ=60)
                               AGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGA                                                                >  SRR4302536.2372732/1‑94 (MQ=60)
                               AGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGA                                                                >  SRR4302536.2231626/1‑94 (MQ=60)
                                GATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGAT                                                               <  SRR4302536.205228/94‑1 (MQ=60)
                                 ATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATG                                                              <  SRR4302536.681833/94‑1 (MQ=60)
                                 ATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATG                                                              >  SRR4302536.5642753/1‑94 (MQ=60)
                                 ATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATG                                                              <  SRR4302536.4449956/94‑1 (MQ=60)
                                   ATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGC                                                            >  SRR4302536.590419/1‑94 (MQ=60)
                                   ATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGC                                                            >  SRR4302536.5575917/1‑94 (MQ=60)
                                   ATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGC                                                            <  SRR4302536.52946/94‑1 (MQ=60)
                                   ATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGC                                                            >  SRR4302536.1379255/1‑94 (MQ=60)
                                    TAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCC                                                           >  SRR4302536.330277/1‑94 (MQ=60)
                                     AAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCG                                                          >  SRR4302536.3616557/1‑94 (MQ=60)
                                      AAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGT                                                         >  SRR4302536.4990204/1‑94 (MQ=60)
                                      AAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGT                                                         >  SRR4302536.1265718/1‑94 (MQ=60)
                                        ATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCC                                                       >  SRR4302536.4689086/1‑94 (MQ=60)
                                         tgctgatgataaaaatgcaggccatcgccgttaCATTGCCGCAGCGATACGGCGCGATATTATCCATGGTGATTGGTATTTCGATGGCCGTCCT                                                      <  SRR4302536.914945/61‑1 (MQ=54)
                                         TTCTTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCT                                                      <  SRR4302536.2427752/94‑1 (MQ=60)
                                         TTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCT                                                      >  SRR4302536.6473308/1‑94 (MQ=60)
                                         TTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCT                                                      <  SRR4302536.2305820/94‑1 (MQ=60)
                                          TCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTT                                                     <  SRR4302536.5491043/94‑1 (MQ=60)
                                          TCCTATGCCAAAAGTTCAGGACGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACTATTGTGATTGGTCTTTCGATGGTCGTTCTT                                                     >  SRR4302536.5158932/1‑94 (MQ=60)
                                            CTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGACGTCCTTGA                                                   >  SRR4302536.2938830/1‑94 (MQ=60)
                                             TATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGAC                                                  <  SRR4302536.6453230/94‑1 (MQ=60)
                                             TATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGAC                                                  >  SRR4302536.6123456/1‑94 (MQ=60)
                                             TATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGAC                                                  >  SRR4302536.3310336/1‑94 (MQ=60)
                                                 CCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCG                                              >  SRR4302536.951566/1‑94 (MQ=60)
                                                 CCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCG                                              >  SRR4302536.1264244/1‑94 (MQ=60)
                                                 CCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCG                                              <  SRR4302536.715394/94‑1 (MQ=60)
                                                 CCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCG                                              <  SRR4302536.6096260/94‑1 (MQ=60)
                                                  CAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGC                                             >  SRR4302536.4823979/1‑94 (MQ=60)
                                                  CAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGC                                             >  SRR4302536.3660987/1‑94 (MQ=60)
                                                   AAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGGCGTCCTTGACGGCGCA                                            >  SRR4302536.6545538/1‑94 (MQ=60)
                                                   AAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCA                                            >  SRR4302536.4184511/1‑94 (MQ=60)
                                                   AAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCA                                            >  SRR4302536.1467143/1‑94 (MQ=60)
                                                   AAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCA                                            >  SRR4302536.3725567/1‑94 (MQ=60)
                                                    AAAGTTTAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAA                                           >  SRR4302536.1628325/1‑94 (MQ=60)
                                                     AAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAAT                                          <  SRR4302536.5885112/94‑1 (MQ=60)
                                                     AAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAAT                                          <  SRR4302536.4358911/94‑1 (MQ=60)
                                                      AGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATC                                         >  SRR4302536.4727392/1‑94 (MQ=60)
                                                          CAGGCCGACGGCCTGTCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCA                                     <  SRR4302536.4168670/94‑1 (MQ=60)
                                                          CAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCA                                     <  SRR4302536.1092130/94‑1 (MQ=60)
                                                          CAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCA                                     >  SRR4302536.3189474/1‑94 (MQ=60)
                                                          CAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCA                                     >  SRR4302536.4403831/1‑94 (MQ=60)
                                                          CAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGCCCGTCCTTGACGGCGCAATCGCCA                                     <  SRR4302536.3742875/94‑1 (MQ=60)
                                                           AGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGGCGTCCTTGACGGCGCGATCGCCAA                                    >  SRR4302536.1775779/1‑94 (MQ=60)
                                                           AGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAA                                    >  SRR4302536.3299135/1‑94 (MQ=60)
                                                           AGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAA                                    >  SRR4302536.4068971/1‑94 (MQ=60)
                                                           AGGCCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAA                                    >  SRR4302536.4620005/1‑94 (MQ=60)
                                                              CCGACGGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGT                                 <  SRR4302536.1512522/94‑1 (MQ=60)
                                                                   GGCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCC                            >  SRR4302536.5655879/1‑94 (MQ=60)
                                                                    GCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCT                           >  SRR4302536.1312604/1‑94 (MQ=60)
                                                                    GCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCT                           >  SRR4302536.4276424/1‑94 (MQ=60)
                                                                    GCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCT                           <  SRR4302536.5688659/94‑1 (MQ=60)
                                                                    GCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCT                           >  SRR4302536.3909124/1‑94 (MQ=60)
                                                                    GCCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGCTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCT                           <  SRR4302536.4069121/94‑1 (MQ=60)
                                                                     CCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTG                          <  SRR4302536.1857577/94‑1 (MQ=60)
                                                                     CCTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTG                          <  SRR4302536.321635/94‑1 (MQ=60)
                                                                      CTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGC                         >  SRR4302536.5761694/1‑94 (MQ=60)
                                                                      CTGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGC                         >  SRR4302536.4076516/1‑94 (MQ=60)
                                                                       TGCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCC                        >  SRR4302536.2405436/1‑94 (MQ=60)
                                                                        GCCATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCA                       >  SRR4302536.3777731/1‑94 (MQ=60)
                                                                          CATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAAC                     >  SRR4302536.5482104/1‑94 (MQ=60)
                                                                           ATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACA                    >  SRR4302536.1920612/1‑94 (MQ=60)
                                                                           ATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACA                    >  SRR4302536.2275577/1‑94 (MQ=60)
                                                                           ATTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACA                    >  SRR4302536.3114915/1‑94 (MQ=60)
                                                                            TTGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAA                   <  SRR4302536.3890895/94‑1 (MQ=60)
                                                                             TGCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAAT                  <  SRR4302536.589568/94‑1 (MQ=60)
                                                                              GCCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATC                 <  SRR4302536.2640263/94‑1 (MQ=60)
                                                                               CCCCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCG                <  SRR4302536.626940/94‑1 (MQ=60)
                                                                                 CCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCC              <  SRR4302536.262662/94‑1 (MQ=60)
                                                                                 CCAGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGACGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCC              >  SRR4302536.5115937/1‑94 (MQ=60)
                                                                                   AGCGATACGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCAC            <  SRR4302536.4105098/94‑1 (MQ=60)
                                                                                        TACGGAGCGATATTAACCATTGTGATTGATATTTCAAAGGACGTCCTTGACGGCGCAATCGCCAACGACGAACTGCCAACAATCGCCAAGGACC       <  SRR4302536.6925011/94‑1 (MQ=53)
                                                                                          CGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTT     >  SRR4302536.4413827/1‑94 (MQ=60)
                                                                                          CGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTT     <  SRR4302536.2759302/94‑1 (MQ=60)
                                                                                          CGGCGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTT     >  SRR4302536.1543229/1‑94 (MQ=60)
                                                                                             CGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGTGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCAT  <  SRR4302536.2618370/94‑1 (MQ=60)
                                                                                             CGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCAT  <  SRR4302536.2568356/94‑1 (MQ=60)
                                                                                             |                                                                                             
TAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCAT  >  NC_000913/1908254‑1908440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑