Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,920,902 T→C Q31R (CAG→CGG)  b1838 ← protein phosphatase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,920,9020TC99.5% 670.8 / NA 209Q31R (CAG→CGG) b1838protein phosphatase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (98/110);  minor base A (1/0);  total (99/110)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

CTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTC  >  NC_000913/1920812‑1920991
                                                                                          |                                                                                         
cTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGc                                                                                         <  2:3038258/93‑1 (MQ=255)
 tttGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGcc                                                                                        >  1:283933/1‑92 (MQ=255)
 tttGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCt                                                                                        <  1:2977279/93‑1 (MQ=255)
  ttGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTc                                                                                       >  1:100019/1‑93 (MQ=255)
   tGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCa                                                                                      <  2:1232527/93‑1 (MQ=255)
    gCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCaa                                                                                     <  2:4257797/93‑1 (MQ=255)
    gCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCaa                                                                                     <  2:507687/93‑1 (MQ=255)
      ggCCCACGATTGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGa                                                                                   >  2:3147517/1‑93 (MQ=255)
      ggCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGa                                                                                   >  1:4219031/1‑93 (MQ=255)
          cACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAAc                                                                               >  1:1598455/1‑93 (MQ=255)
          cACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAAc                                                                               <  1:1252998/93‑1 (MQ=255)
           aCGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAAcc                                                                              <  1:2724355/93‑1 (MQ=255)
            cgatcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCg                                                                             >  2:4576160/1‑93 (MQ=255)
             gatcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgt                                                                            >  1:4036619/1‑93 (MQ=255)
              atcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCGACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgtg                                                                           <  2:3424603/93‑1 (MQ=255)
              atcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgtg                                                                           >  2:4698915/1‑93 (MQ=255)
              atcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgtg                                                                           <  2:1672080/93‑1 (MQ=255)
                  atAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGtata                                                                       <  1:3104540/93‑1 (MQ=255)
                  atAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGtata                                                                       >  2:3463810/1‑93 (MQ=255)
                  atAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGtata                                                                       >  1:3026114/1‑93 (MQ=255)
                   tAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTatat                                                                      >  2:2263693/1‑93 (MQ=255)
                   tAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGAGTatat                                                                      >  2:922094/1‑93 (MQ=255)
                   tAACGCCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTatat                                                                      <  1:3540897/93‑1 (MQ=255)
                    aaCGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATc                                                                     <  2:646828/93‑1 (MQ=255)
                     aCGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCg                                                                    >  2:1588036/1‑93 (MQ=255)
                      cgcctcCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGc                                                                   <  1:3705154/90‑1 (MQ=255)
                      cGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGc                                                                   <  1:489108/93‑1 (MQ=255)
                      cGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGc                                                                   >  1:3906358/1‑93 (MQ=255)
                       gTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGcc                                                                  >  1:97835/1‑93 (MQ=255)
                       gTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGcc                                                                  <  2:4477557/93‑1 (MQ=255)
                       gTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGcc                                                                  <  2:247810/93‑1 (MQ=255)
                       gTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGcc                                                                  >  2:2980344/1‑93 (MQ=255)
                       gTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGcc                                                                  >  2:391527/1‑93 (MQ=255)
                        tctcCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCa                                                                 <  2:1226157/93‑1 (MQ=255)
                          tcCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGa                                                               >  1:182393/1‑93 (MQ=255)
                          tcCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGa                                                               >  2:4307795/1‑93 (MQ=255)
                          tcCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGa                                                               <  1:2643913/93‑1 (MQ=255)
                             cACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAg                                                            >  1:642914/1‑93 (MQ=255)
                              aCTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGc                                                           <  1:376366/93‑1 (MQ=255)
                                 gagaTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAg                                                        <  2:4134350/93‑1 (MQ=255)
                                   gaTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGat                                                      <  1:1401989/93‑1 (MQ=255)
                                   gaTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGat                                                      >  1:4271378/1‑93 (MQ=255)
                                    aTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGata                                                     <  2:2898031/93‑1 (MQ=255)
                                    aTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGata                                                     <  2:223554/93‑1 (MQ=255)
                                    aTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGata                                                     <  1:3232773/93‑1 (MQ=255)
                                    aTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGata                                                     <  1:665051/93‑1 (MQ=255)
                                    aTAAGTAAATCGCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAAACGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGata                                                     <  1:317310/93‑1 (MQ=255)
                                     tAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGTGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGatat                                                    <  2:1473147/93‑1 (MQ=255)
                                     tAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGatat                                                    <  2:206111/93‑1 (MQ=255)
                                      aaGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCATGACAACCGTGTATATCGCCAGAAAGCCAGATATg                                                   <  1:4189940/93‑1 (MQ=255)
                                      aaGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATg                                                   <  2:1783986/93‑1 (MQ=255)
                                       aGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGt                                                  >  2:4436128/1‑93 (MQ=255)
                                          aaaTCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGc                                               <  2:316958/93‑1 (MQ=255)
                                             tctcGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGGCAt                                            <  2:4645679/93‑1 (MQ=255)
                                              ctcGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCAtt                                           <  2:3965499/93‑1 (MQ=255)
                                               tcGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtg                                          >  1:4489326/1‑93 (MQ=255)
                                               ccGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtg                                          <  2:1139373/92‑1 (MQ=255)
                                                 gCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtgat                                        <  1:227439/93‑1 (MQ=255)
                                                  ccACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtgatg                                       >  1:314503/1‑93 (MQ=255)
                                                   cACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtgatga                                      <  2:539750/93‑1 (MQ=255)
                                                   cACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtgatga                                      >  1:2097979/1‑93 (MQ=255)
                                                   cACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtgatga                                      >  1:1456564/1‑93 (MQ=255)
                                                    aCGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGAc                                     >  1:3337929/1‑93 (MQ=255)
                                                          cAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCaa                               >  2:3117935/1‑93 (MQ=255)
                                                           aaaaCGACAATGCCATAATTTGCGGCGCAACCCCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAt                              <  2:3637207/93‑1 (MQ=255)
                                                            aaaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAtt                             >  2:1950555/1‑93 (MQ=255)
                                                              aCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATtct                           <  1:1210975/93‑1 (MQ=255)
                                                               cGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATtctc                          >  2:1859396/1‑93 (MQ=255)
                                                               cGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATtctc                          <  1:2953599/93‑1 (MQ=255)
                                                                gACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTctct                         <  2:2814864/93‑1 (MQ=255)
                                                                 aCAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTg                        >  2:2170896/1‑93 (MQ=255)
                                                                  cAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGa                       <  1:135264/93‑1 (MQ=255)
                                                                  cAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGa                       <  2:740084/93‑1 (MQ=255)
                                                                  cAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGa                       >  1:1310575/1‑93 (MQ=255)
                                                                  cAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGa                       <  2:767341/93‑1 (MQ=255)
                                                                   aaTGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGAt                      <  2:1666782/93‑1 (MQ=255)
                                                                    aTGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATa                     >  2:1515027/1‑93 (MQ=255)
                                                                     tGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATaa                    >  2:4724118/1‑93 (MQ=255)
                                                                       ccATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAAc                  <  2:2680669/93‑1 (MQ=255)
                                                                         ctAATTTGCGGCGCAGCCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACgg                <  2:577610/92‑1 (MQ=255)
                                                                         aTAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACgg                <  1:1768305/93‑1 (MQ=255)
                                                                         aTAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACgg                <  2:4204310/93‑1 (MQ=255)
                                                                          tAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACggg               <  2:3886612/93‑1 (MQ=255)
                                                                          tAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACggg               <  1:2232955/93‑1 (MQ=255)
                                                                          tAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACggg               <  1:4595707/93‑1 (MQ=255)
                                                                          tAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACggg               <  1:4683088/93‑1 (MQ=255)
                                                                           aaTTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGgc              >  1:3420236/1‑93 (MQ=255)
                                                                             tttGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGgcgc            >  1:3496360/1‑93 (MQ=255)
                                                                             tttGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGgcgc            >  1:2405387/1‑93 (MQ=255)
                                                                             tttGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGgcgc            <  2:489260/93‑1 (MQ=255)
                                                                              ttGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCa           <  1:3888692/93‑1 (MQ=255)
                                                                              ttGCGGCGCAACCGCGCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGCCCCGCAATTCTCTGATAAACGGGCGCa           <  1:688971/93‑1 (MQ=255)
                                                                               tGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAg          <  2:1901612/93‑1 (MQ=255)
                                                                                gcggcgCAACCACTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAgg         >  2:3769027/1‑93 (MQ=255)
                                                                                 cggcgCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGc        <  2:1380287/93‑1 (MQ=255)
                                                                                 cggcgCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGc        <  1:2492299/93‑1 (MQ=255)
                                                                                     gcAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGtt    <  1:1988206/93‑1 (MQ=255)
                                                                                      cAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGttt   <  1:576125/93‑1 (MQ=255)
                                                                                       aaCCGCTCAAGACATCCGTGTATATCGTCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTtc  <  2:213981/93‑1 (MQ=255)
                                                                                       aaCCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTtc  >  1:1040224/1‑93 (MQ=255)
                                                                                          |                                                                                         
CTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTC  >  NC_000913/1920812‑1920991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCA  >  NC_000913/1920809‑1920992
                                                                                             |                                                                                          
AAACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACC                                                                                            <  SRR4302544.1312255/94‑1 (MQ=60)
  ACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGC                                                                                          >  SRR4302544.1048599/1‑94 (MQ=60)
  ACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGC                                                                                          <  SRR4302544.4414363/94‑1 (MQ=60)
    TTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTC                                                                                        <  SRR4302544.3005837/94‑1 (MQ=60)
    TTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTC                                                                                        >  SRR4302544.100938/1‑94 (MQ=60)
      TGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAA                                                                                      <  SRR4302544.95496/94‑1 (MQ=60)
        CGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGA                                                                                    >  SRR4302544.3486687/1‑94 (MQ=60)
        CGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGA                                                                                    >  SRR4302544.4261738/1‑94 (MQ=60)
         GGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGAC                                                                                   >  SRR4302544.1358956/1‑94 (MQ=60)
         GGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGAC                                                                                   >  SRR4302544.831623/1‑94 (MQ=60)
          GCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACAGCTCAAGACA                                                                                  >  SRR4302544.2774959/1‑94 (MQ=60)
            CCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAAC                                                                                >  SRR4302544.3418090/1‑94 (MQ=60)
            CCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAAC                                                                                >  SRR4302544.1614211/1‑94 (MQ=60)
             CACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACC                                                                               <  SRR4302544.1265327/94‑1 (MQ=60)
              ACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCG                                                                              <  SRR4302544.2750588/94‑1 (MQ=60)
               CGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGT                                                                             >  SRR4302544.4076821/1‑94 (MQ=60)
                GATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTG                                                                            >  SRR4302544.3766268/1‑94 (MQ=60)
                GATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTG                                                                            >  SRR4302544.2428596/1‑94 (MQ=60)
                  TCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTA                                                                          >  SRR4302544.2440076/1‑94 (MQ=60)
                  TCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTA                                                                          >  SRR4302544.227790/1‑94 (MQ=60)
                   CGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTAT                                                                         <  SRR4302544.3233385/94‑1 (MQ=60)
                    GATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATA                                                                        >  SRR4302544.3055106/1‑94 (MQ=60)
                    GATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATA                                                                        <  SRR4302544.4244722/94‑1 (MQ=60)
                     ATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATAT                                                                       <  SRR4302544.2631046/94‑1 (MQ=60)
                     ATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATAT                                                                       <  SRR4302544.3134088/94‑1 (MQ=60)
                     ATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATAT                                                                       >  SRR4302544.4492270/1‑94 (MQ=60)
                     ATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATAT                                                                       >  SRR4302544.508396/1‑94 (MQ=60)
                     ATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATAT                                                                       >  SRR4302544.434796/1‑94 (MQ=60)
                      TAACGCCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATC                                                                      <  SRR4302544.3574788/94‑1 (MQ=60)
                       AACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCG                                                                     >  SRR4302544.139450/1‑94 (MQ=60)
                       AACGTCCCCCACTGAGATAAGTAACTCTCGCCACGGCTCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCG                                                                     <  SRR4302544.125186/94‑1 (MQ=60)
                        ACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGC                                                                    >  SRR4302544.3945172/1‑94 (MQ=60)
                         CGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCC                                                                   <  SRR4302544.493902/94‑1 (MQ=60)
                         CGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCC                                                                   >  SRR4302544.98732/1‑94 (MQ=60)
                         CGCCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCC                                                                   <  SRR4302544.3742026/94‑1 (MQ=60)
                            CTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGA                                                                >  SRR4302544.184197/1‑94 (MQ=60)
                             TCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAA                                                               <  SRR4302544.2669262/94‑1 (MQ=60)
                              CCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAA                                                              >  SRR4302544.4683177/1‑94 (MQ=60)
                               CCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAG                                                             >  SRR4302544.610574/1‑94 (MQ=60)
                               CCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAG                                                             <  SRR4302544.3151940/94‑1 (MQ=60)
                               CCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAG                                                             >  SRR4302544.649265/1‑94 (MQ=60)
                                 ACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCC                                                           <  SRR4302544.380204/94‑1 (MQ=60)
                                  CTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCA                                                          >  SRR4302544.4744197/1‑94 (MQ=60)
                                     AGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGAT                                                       >  SRR4302544.4314980/1‑94 (MQ=60)
                                      GATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATA                                                      <  SRR4302544.1415955/94‑1 (MQ=60)
                                       ATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATAT                                                     <  SRR4302544.671617/94‑1 (MQ=60)
                                       ATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATAT                                                     <  SRR4302544.320608/94‑1 (MQ=60)
                                       ATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATAT                                                     <  SRR4302544.671618/94‑1 (MQ=60)
                                       ATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATAT                                                     <  SRR4302544.3263712/94‑1 (MQ=60)
                                       ATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATAT                                                     <  SRR4302544.3263713/94‑1 (MQ=60)
                                       ATAAGTAAATCGCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAAACGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATAT                                                     <  SRR4302544.320610/94‑1 (MQ=60)
                                         AAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCATGACAACCGTGTATATCGCCAGAAAGCCAGATATGT                                                   <  SRR4302544.4231444/94‑1 (MQ=60)
                                         AAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGT                                                   >  SRR4302544.4591468/1‑94 (MQ=60)
                                         AAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGT                                                   >  SRR4302544.1829891/1‑94 (MQ=60)
                                           GTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCG                                                 >  SRR4302544.1511952/1‑94 (MQ=60)
                                                 CTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTG                                           >  SRR4302544.4534404/1‑94 (MQ=60)
                                                    GCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATG                                        >  SRR4302544.317770/1‑94 (MQ=60)
                                                    GCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATG                                        <  SRR4302544.229799/94‑1 (MQ=60)
                                                     CCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGA                                       >  SRR4302544.2118164/1‑94 (MQ=60)
                                                     CCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGA                                       >  SRR4302544.1471030/1‑94 (MQ=60)
                                                     CCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGA                                       >  SRR4302544.1471029/1‑94 (MQ=60)
                                                      CACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGAC                                      >  SRR4302544.3369733/1‑94 (MQ=60)
                                                          GATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGC                                  <  SRR4302544.1659397/94‑1 (MQ=60)
                                                           ATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCA                                 >  SRR4302544.1116787/1‑94 (MQ=60)
                                                              AAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATT                              <  SRR4302544.2734908/94‑1 (MQ=60)
                                                                AACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCT                            <  SRR4302544.1846139/94‑1 (MQ=60)
                                                                AACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCT                            <  SRR4302544.2883441/94‑1 (MQ=60)
                                                                 ACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTC                           <  SRR4302544.1222964/94‑1 (MQ=60)
                                                                  CGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCT                          <  SRR4302544.2421365/94‑1 (MQ=60)
                                                                  CGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCT                          <  SRR4302544.2981970/94‑1 (MQ=60)
                                                                   GACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTG                         <  SRR4302544.3928529/94‑1 (MQ=60)
                                                                    ACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGA                        >  SRR4302544.1323458/1‑94 (MQ=60)
                                                                     CAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGAT                       >  SRR4302544.2982166/1‑94 (MQ=60)
                                                                     CAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGAT                       <  SRR4302544.136538/94‑1 (MQ=60)
                                                                      AATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATA                      >  SRR4302544.4096510/1‑94 (MQ=60)
                                                                        TGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAA                    >  SRR4302544.4026892/1‑94 (MQ=60)
                                                                         GCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAAC                   <  SRR4302544.3664201/94‑1 (MQ=60)
                                                                          CCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACG                  >  SRR4302544.3864548/1‑94 (MQ=60)
                                                                          CCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACG                  >  SRR4302544.1563690/1‑94 (MQ=60)
                                                                          CCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACG                  <  SRR4302544.3550091/94‑1 (MQ=60)
                                                                          CCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACG                  <  SRR4302544.2031109/94‑1 (MQ=60)
                                                                          CCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACG                  <  SRR4302544.3147515/94‑1 (MQ=60)
                                                                            ATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGG                >  SRR4302544.3093301/1‑94 (MQ=60)
                                                                            ATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGG                <  SRR4302544.1785614/94‑1 (MQ=60)
                                                                             TAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGC               <  SRR4302544.2253956/94‑1 (MQ=60)
                                                                             TAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGC               <  SRR4302544.4729380/94‑1 (MQ=60)
                                                                             TAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGC               <  SRR4302544.4641416/94‑1 (MQ=60)
                                                                             TAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGC               >  SRR4302544.3452939/1‑94 (MQ=60)
                                                                              AATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGCGCG              >  SRR4302544.2184450/1‑94 (MQ=60)
                                                                               ATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGC             >  SRR4302544.2428221/1‑94 (MQ=60)
                                                                               ATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGC             >  SRR4302544.3529697/1‑94 (MQ=60)
                                                                                TTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCA            <  SRR4302544.3617530/94‑1 (MQ=60)
                                                                                 TTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAG           <  SRR4302544.3927319/94‑1 (MQ=60)
                                                                                 TTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAG           <  SRR4302544.1294709/94‑1 (MQ=60)
                                                                                 TTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAG           <  SRR4302544.695828/94‑1 (MQ=60)
                                                                                 TTGCGGCGCAACCGCGCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGCCCCGCAATTCTCTGATAAACGGGCGCAG           <  SRR4302544.695829/94‑1 (MQ=60)
                                                                                    CGGCGCAACCGCTCAAGCCAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCT        <  SRR4302544.4268609/94‑1 (MQ=60)
                                                                                    CGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCT        <  SRR4302544.2516166/94‑1 (MQ=60)
                                                                                        GCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTT    <  SRR4302544.2007507/94‑1 (MQ=60)
                                                                                         CAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTC   <  SRR4302544.581756/94‑1 (MQ=60)
                                                                                         CAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTC   >  SRR4302544.1050549/1‑94 (MQ=60)
                                                                                          AACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCA  <  SRR4302544.3051993/94‑1 (MQ=60)
                                                                                          AACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCA  >  SRR4302544.999289/1‑94 (MQ=60)
                                                                                             |                                                                                          
AAACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCA  >  NC_000913/1920809‑1920992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑