Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,920,902 T→C Q31R (CAG→CGG)  b1838 ← protein phosphatase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,920,9020TC99.6% 730.5 / ‑5.1 228Q31R (CAG→CGG) b1838protein phosphatase 1
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (117/110);  total (117/111)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

Only 100 of 232 total aligned reads displayed.

CTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTC  >  NC_000913/1920812‑1920991
                                                                                          |                                                                                         
 tttGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCt                                                                                        <  1:4313016/93‑1 (MQ=255)
  ttGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTc                                                                                       >  1:4697092/1‑93 (MQ=255)
       gCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGAc                                                                                  <  2:3988329/93‑1 (MQ=255)
        cccACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACa                                                                                 <  1:3296530/93‑1 (MQ=255)
          cACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAAc                                                                               <  2:876904/93‑1 (MQ=255)
          cACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAAc                                                                               >  2:3135779/1‑93 (MQ=255)
          cACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCCCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAAc                                                                               >  1:1769016/1‑93 (MQ=255)
           aCGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAAcc                                                                              >  2:2525116/1‑93 (MQ=255)
            cgatcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCg                                                                             <  2:5985845/93‑1 (MQ=255)
             gatcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgt                                                                            >  1:5416085/1‑93 (MQ=255)
             gatcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgt                                                                            >  1:3413498/1‑93 (MQ=255)
             gatcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgg                                                                            >  2:2885171/1‑92 (MQ=255)
              atcgatAACGTCTCCCACTGTGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgtg                                                                           >  1:969537/1‑93 (MQ=255)
              atcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgtg                                                                           <  2:1808787/93‑1 (MQ=255)
              atcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgtg                                                                           <  2:3404741/93‑1 (MQ=255)
              atcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgtg                                                                           >  1:1722840/1‑93 (MQ=255)
               tcgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCgtgt                                                                          <  1:1895192/93‑1 (MQ=255)
                cgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGta                                                                         <  2:3962420/93‑1 (MQ=255)
                cgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGta                                                                         >  2:1400462/1‑93 (MQ=255)
                cgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGta                                                                         <  1:3566380/93‑1 (MQ=255)
                cgatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGta                                                                         <  2:3872326/93‑1 (MQ=255)
                 gatAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGtat                                                                        >  1:3889895/1‑93 (MQ=255)
                  atAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGtata                                                                       >  2:6173634/1‑93 (MQ=255)
                  atAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGtata                                                                       >  2:3762126/1‑93 (MQ=255)
                   tAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTatat                                                                      >  1:2424353/1‑93 (MQ=255)
                    aaCGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGAGAACCGTGTATATc                                                                     >  1:821454/1‑93 (MQ=255)
                    aaCGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATc                                                                     >  1:1568086/1‑93 (MQ=255)
                     aCGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCg                                                                    >  1:3476266/1‑93 (MQ=255)
                     aCGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCg                                                                    >  1:5011253/1‑93 (MQ=255)
                        tctcCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCa                                                                 <  2:4526295/93‑1 (MQ=255)
                        tctcCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCa                                                                 >  2:3442199/1‑93 (MQ=255)
                         ctcCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAg                                                                >  2:650997/1‑93 (MQ=255)
                            ccACTGAGATAAGTAAATCTCGCCACGTATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGaaa                                                             <  2:2907627/93‑1 (MQ=255)
                            ccACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGaaa                                                             <  2:3240614/93‑1 (MQ=255)
                               cTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGcc                                                          <  2:1432752/93‑1 (MQ=255)
                               cTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGcc                                                          >  1:1424082/1‑93 (MQ=255)
                                tGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCa                                                         >  2:3273539/1‑93 (MQ=255)
                                 gagaTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAg                                                        >  1:117813/1‑93 (MQ=255)
                                  agaTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGTAAGCCAGa                                                       <  1:6564848/93‑1 (MQ=255)
                                  agaTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGa                                                       <  1:4847074/93‑1 (MQ=255)
                                  agaTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGa                                                       >  2:4991144/1‑93 (MQ=255)
                                  agaTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGGGTATATCGCCAGAAAGCCAGa                                                       >  1:5066485/1‑93 (MQ=255)
                                   gaTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGat                                                      <  1:5259237/93‑1 (MQ=255)
                                   gaTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGat                                                      <  2:1268651/93‑1 (MQ=255)
                                    aTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGata                                                     <  1:2078651/93‑1 (MQ=255)
                                    aTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGata                                                     <  1:6241099/93‑1 (MQ=255)
                                     tAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGatat                                                    <  2:4764020/93‑1 (MQ=255)
                                     tAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGatat                                                    <  1:5674725/93‑1 (MQ=255)
                                       aGTAAATCTCGCCGCGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGt                                                  <  2:1335252/93‑1 (MQ=255)
                                       aGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGt                                                  >  1:3002110/1‑93 (MQ=255)
                                        gTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTc                                                 >  1:1539560/1‑93 (MQ=255)
                                        gTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTc                                                 <  2:4193195/93‑1 (MQ=255)
                                        gTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTc                                                 >  1:2717214/1‑93 (MQ=255)
                                            aTCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCa                                             <  2:724826/93‑1 (MQ=255)
                                            aTCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGGGTATATCGCCAGAAAGCCAGATATGTCGcct                                             >  1:4967606/1‑92 (MQ=255)
                                             tctcGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCCAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCAt                                            <  2:4697456/93‑1 (MQ=255)
                                              ctcGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCAtt                                           <  1:1337112/93‑1 (MQ=255)
                                              ctcGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCAtt                                           <  2:2302520/93‑1 (MQ=255)
                                               tcGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtg                                          >  2:585112/1‑93 (MQ=255)
                                               tcGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATAGCGCCAGAAAGCCAGATATGTCGCCATtg                                          >  2:6450355/1‑93 (MQ=255)
                                                cGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtga                                         <  2:1471283/93‑1 (MQ=255)
                                                 gCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtgat                                        >  1:3236394/1‑93 (MQ=255)
                                                   cACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtgatga                                      >  2:5391896/1‑93 (MQ=255)
                                                   cACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATtgatga                                      <  2:2159843/93‑1 (MQ=255)
                                                     cGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGAcc                                    <  1:4028810/93‑1 (MQ=255)
                                                      ggATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGAccc                                   >  2:5783747/1‑93 (MQ=255)
                                                        aTCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGc                                 >  1:2463900/1‑93 (MQ=255)
                                                         tCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCa                                >  1:2658750/1‑93 (MQ=255)
                                                          cAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCaa                               <  1:4253154/93‑1 (MQ=255)
                                                           aaaaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAt                              >  1:4149680/1‑93 (MQ=255)
                                                           aaaaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAt                              >  2:5107862/1‑93 (MQ=255)
                                                           aaaaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAt                              >  2:1168558/1‑93 (MQ=255)
                                                           aaaaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAt                              >  1:4593995/1‑93 (MQ=255)
                                                            aaaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAtt                             >  2:2742245/1‑93 (MQ=255)
                                                            aaaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAtt                             >  1:917578/1‑93 (MQ=255)
                                                             aaCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATtc                            >  1:685559/1‑93 (MQ=255)
                                                              aCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATtct                           >  2:3521853/1‑93 (MQ=255)
                                                              aCGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATtct                           >  2:112132/1‑93 (MQ=255)
                                                                gACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTctct                         <  1:6222261/93‑1 (MQ=255)
                                                                 aCAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTg                        >  1:5766958/1‑93 (MQ=255)
                                                                  cAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGa                       >  2:1080656/1‑93 (MQ=255)
                                                                   aaTGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGGCCCGCAATTCTCTGAt                      <  1:437920/93‑1 (MQ=255)
                                                                   aaTGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGAt                      <  1:2537165/93‑1 (MQ=255)
                                                                   aaTGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGAt                      >  1:1834965/1‑93 (MQ=255)
                                                                   aaTGCCATAATGTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGAt                      <  1:2226032/93‑1 (MQ=255)
                                                                     tGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATaa                    <  1:5974360/93‑1 (MQ=255)
                                                                       ccATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAAc                  <  2:5237955/93‑1 (MQ=255)
                                                                        cATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACg                 >  2:4028026/1‑93 (MQ=255)
                                                                        cATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACg                 >  2:6355085/1‑93 (MQ=255)
                                                                         aTAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACgg                <  1:1610289/93‑1 (MQ=255)
                                                                         aTAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACgg                <  2:5579120/93‑1 (MQ=255)
                                                                          tAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACggg               <  1:52978/93‑1 (MQ=255)
                                                                            aTTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGgcg             >  2:1657020/1‑93 (MQ=255)
                                                                             tttGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGgcgc            >  1:6118300/1‑93 (MQ=255)
                                                                              ttGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCa           <  2:3901573/93‑1 (MQ=255)
                                                                                 cggcgCAACCGCTCAAGACAGCCGTGTATATCGCCAGAAAGCCAGATATGTCGCCGTTGATGGCCCGCAATTCTCTGATAAACGGGCGCAGGc        <  1:3188067/93‑1 (MQ=255)
                                                                                  ggcgCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCt       <  2:3717553/93‑1 (MQ=255)
                                                                                    cgcAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGt     >  1:4011493/1‑93 (MQ=255)
                                                                                       aaCCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTtc  >  2:1699623/1‑93 (MQ=255)
                                                                                       aaCCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTtc  >  1:1079537/1‑93 (MQ=255)
                                                                                          |                                                                                         
CTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTC  >  NC_000913/1920812‑1920991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCAT  >  NC_000913/1920809‑1920993
                                                                                             |                                                                                           
AAACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACC                                                                                             <  SRR4302536.6568654/94‑1 (MQ=60)
AAACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACC                                                                                             <  SRR4302536.2167721/94‑1 (MQ=60)
AAACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACC                                                                                             <  SRR4302536.2028982/94‑1 (MQ=60)
AAACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACC                                                                                             <  SRR4302536.5184013/94‑1 (MQ=60)
AAACTTTGCGGCCCACGATCGATAACGTCTCCCACCGAGATAAGTAAATCTCGCGACGGATCAAAACGACAATGCCATCATTTGCGGGGCAACC                                                                                             >  SRR4302536.2061328/1‑94 (MQ=60)
 AACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCG                                                                                            <  SRR4302536.3521861/94‑1 (MQ=60)
 AACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCG                                                                                            <  SRR4302536.2291410/94‑1 (MQ=60)
   CTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCT                                                                                          >  SRR4302536.14701/1‑94 (MQ=60)
   CTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACAACAATGCCATAATTTGCGGCGCAACCGCT                                                                                          <  SRR4302536.5118666/94‑1 (MQ=60)
   CTTTGCGACCCACGAACGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCT                                                                                          <  SRR4302536.1020299/94‑1 (MQ=60)
    TTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTC                                                                                         <  SRR4302536.4442105/94‑1 (MQ=60)
    TTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTC                                                                                         >  SRR4302536.4838326/1‑94 (MQ=60)
      TGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAA                                                                                       >  SRR4302536.2572279/1‑94 (MQ=60)
      TGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAA                                                                                       <  SRR4302536.184943/94‑1 (MQ=60)
        CGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGA                                                                                     >  SRR4302536.5968805/1‑94 (MQ=60)
           CCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAA                                                                                  <  SRR4302536.3393639/94‑1 (MQ=60)
            CCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCCCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAAC                                                                                 >  SRR4302536.1822549/1‑94 (MQ=60)
              ACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCG                                                                               >  SRR4302536.3523552/1‑94 (MQ=60)
               CGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGT                                                                              >  SRR4302536.3515068/1‑94 (MQ=60)
               CGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGT                                                                              >  SRR4302536.5577956/1‑94 (MQ=60)
                GATCGATAACGTCTCCCACTGTGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTG                                                                             >  SRR4302536.998152/1‑94 (MQ=60)
                GATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTG                                                                             <  SRR4302536.5207915/94‑1 (MQ=60)
                GATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTG                                                                             <  SRR4302536.1765350/94‑1 (MQ=60)
                GATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTG                                                                             >  SRR4302536.1772606/1‑94 (MQ=60)
                 ATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGT                                                                            <  SRR4302536.4047087/94‑1 (MQ=60)
                 ATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGT                                                                            <  SRR4302536.538084/94‑1 (MQ=60)
                  TCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTA                                                                           <  SRR4302536.412441/94‑1 (MQ=60)
                  TCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTA                                                                           <  SRR4302536.1952239/94‑1 (MQ=60)
                   CGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTAT                                                                          <  SRR4302536.3673013/94‑1 (MQ=60)
                   CGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTAT                                                                          <  SRR4302536.1604063/94‑1 (MQ=60)
                   CGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTAT                                                                          >  SRR4302536.4005568/1‑94 (MQ=60)
                   CGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTAT                                                                          >  SRR4302536.528724/1‑94 (MQ=60)
                    GATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATA                                                                         <  SRR4302536.944510/94‑1 (MQ=60)
                    GATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATA                                                                         >  SRR4302536.1928088/1‑94 (MQ=60)
                     ATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATAT                                                                        >  SRR4302536.3634781/1‑94 (MQ=60)
                     ATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATAT                                                                        <  SRR4302536.6177081/94‑1 (MQ=60)
                     ATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATAT                                                                        >  SRR4302536.2497645/1‑94 (MQ=60)
                      TAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGAGAACCGTGTATATC                                                                       >  SRR4302536.845628/1‑94 (MQ=60)
                      TAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATC                                                                       >  SRR4302536.1614086/1‑94 (MQ=60)
                       AACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCG                                                                      <  SRR4302536.2683390/94‑1 (MQ=60)
                       AACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCG                                                                      >  SRR4302536.3579885/1‑94 (MQ=60)
                       AACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCG                                                                      >  SRR4302536.5161552/1‑94 (MQ=60)
                       AACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCG                                                                      >  SRR4302536.3159139/1‑94 (MQ=60)
                           gccCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAG                                                                  <  SRR4302536.1744023/91‑1 (MQ=60)
                           TCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAG                                                                  <  SRR4302536.3426161/94‑1 (MQ=60)
                            CTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGA                                                                 >  SRR4302536.4485434/1‑94 (MQ=60)
                              CCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAA                                                               >  SRR4302536.892403/1‑94 (MQ=60)
                               CCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATGTGCGGCGCAACCCCTCAAGACAACCGTGTATATCGCCAGAAAG                                                              <  SRR4302536.2527284/94‑1 (MQ=60)
                                CACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGC                                                             >  SRR4302536.2386841/1‑94 (MQ=60)
                                CACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGC                                                             >  SRR4302536.4379311/1‑94 (MQ=60)
                                 ACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCC                                                            >  SRR4302536.1466398/1‑94 (MQ=60)
                                  CTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCA                                                           <  SRR4302536.487751/94‑1 (MQ=60)
                                   TGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAG                                                          >  SRR4302536.120911/1‑94 (MQ=60)
                                    GAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGGGTATATCGCCAGAAAGCCAGA                                                         >  SRR4302536.5218214/1‑94 (MQ=60)
                                     AGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGTAAGCCAGAT                                                        <  SRR4302536.6760248/94‑1 (MQ=60)
                                     AGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGAT                                                        >  SRR4302536.1121061/1‑94 (MQ=60)
                                     AGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGAT                                                        <  SRR4302536.4992936/94‑1 (MQ=60)
                                     AGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGAT                                                        >  SRR4302536.4678047/1‑94 (MQ=60)
                                      GATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATA                                                       <  SRR4302536.5415966/94‑1 (MQ=60)
                                       ATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATAT                                                      <  SRR4302536.2141244/94‑1 (MQ=60)
                                       ATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATAT                                                      <  SRR4302536.6427020/94‑1 (MQ=60)
                                        TAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATG                                                     <  SRR4302536.5843429/94‑1 (MQ=60)
                                         AAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGT                                                    >  SRR4302536.3090393/1‑94 (MQ=60)
                                          AGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTC                                                   >  SRR4302536.1584832/1‑94 (MQ=60)
                                          AGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTC                                                   >  SRR4302536.2798254/1‑94 (MQ=60)
                                            TAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGC                                                 >  SRR4302536.950620/1‑94 (MQ=60)
                                            TAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGC                                                 >  SRR4302536.4773407/1‑94 (MQ=60)
                                              AATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCA                                               >  SRR4302536.5886375/1‑94 (MQ=60)
                                              AATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCA                                               >  SRR4302536.4542872/1‑94 (MQ=60)
                                              AATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGGGTATATCGCCAGAAAGCCAGATATGTCGCCT                                               >  SRR4302536.5116828/1‑94 (MQ=60)
                                                 CTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTG                                            <  SRR4302536.1377000/94‑1 (MQ=60)
                                                   CGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGAT                                          >  SRR4302536.3331264/1‑94 (MQ=60)
                                                    GCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATG                                         <  SRR4302536.6653662/94‑1 (MQ=60)
                                                    GCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATG                                         <  SRR4302536.4183129/94‑1 (MQ=60)
                                                     CCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGA                                        >  SRR4302536.2682557/1‑94 (MQ=60)
                                                      CACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGAC                                       >  SRR4302536.2764071/1‑94 (MQ=60)
                                                       ACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACC                                      <  SRR4302536.5451762/94‑1 (MQ=60)
                                                        CGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCC                                     <  SRR4302536.4148307/94‑1 (MQ=60)
                                                        CGGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCC                                     >  SRR4302536.3319631/1‑94 (MQ=60)
                                                         GGATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCG                                    >  SRR4302536.1372140/1‑94 (MQ=60)
                                                         GAATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCG                                    <  SRR4302536.1895725/94‑1 (MQ=60)
                                                          GATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGC                                   >  SRR4302536.2538298/1‑94 (MQ=60)
                                                           ATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCA                                  >  SRR4302536.2738331/1‑94 (MQ=60)
                                                           ATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCA                                  <  SRR4302536.6659162/94‑1 (MQ=60)
                                                           ATCAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCA                                  >  SRR4302536.2029372/1‑94 (MQ=60)
                                                             CAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAT                                >  SRR4302536.4731903/1‑94 (MQ=60)
                                                             CAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAT                                <  SRR4302536.4379873/94‑1 (MQ=60)
                                                             CAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAT                                >  SRR4302536.6192703/1‑94 (MQ=60)
                                                             CAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAAT                                >  SRR4302536.4272825/1‑94 (MQ=60)
                                                             CAAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCACTGATGACCCGCAAT                                >  SRR4302536.4282181/1‑94 (MQ=60)
                                                              AAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATT                               >  SRR4302536.944627/1‑94 (MQ=60)
                                                              AAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATT                               <  SRR4302536.2981634/94‑1 (MQ=60)
                                                              AAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATT                               >  SRR4302536.838745/1‑94 (MQ=60)
                                                              AAAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATT                               >  SRR4302536.6411721/1‑94 (MQ=60)
                                                               AAACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTC                              >  SRR4302536.705635/1‑94 (MQ=60)
                                                                AACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCT                             <  SRR4302536.2377483/94‑1 (MQ=60)
                                                                AACGACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCT                             <  SRR4302536.6870593/94‑1 (MQ=60)
                                                                   GACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTG                          >  SRR4302536.5938529/1‑94 (MQ=60)
                                                                   GACAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTG                          <  SRR4302536.6407661/94‑1 (MQ=60)
                                                                     CAATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGAT                        >  SRR4302536.1890279/1‑94 (MQ=60)
                                                                      AATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGGCCCGCAATTCTCTGATA                       <  SRR4302536.449422/94‑1 (MQ=60)
                                                                      AATGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATA                       <  SRR4302536.2613601/94‑1 (MQ=60)
                                                                      AATGCCATAATGTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATA                       <  SRR4302536.2293362/94‑1 (MQ=60)
                                                                        TGCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAA                     <  SRR4302536.6152459/94‑1 (MQ=60)
                                                                         GCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAAC                    >  SRR4302536.553063/1‑94 (MQ=60)
                                                                         GCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAAC                    <  SRR4302536.5692530/94‑1 (MQ=60)
                                                                         GCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAAC                    <  SRR4302536.1414414/94‑1 (MQ=60)
                                                                         GCCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAAC                    <  SRR4302536.1794287/94‑1 (MQ=60)
                                                                          CCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACG                   >  SRR4302536.2206913/1‑94 (MQ=60)
                                                                          CCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACG                   <  SRR4302536.5085990/94‑1 (MQ=60)
                                                                          CCATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACG                   >  SRR4302536.3194716/1‑94 (MQ=60)
                                                                           CATAATTTGCGGCGCAACCTCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGG                  <  SRR4302536.3424445/94‑1 (MQ=60)
                                                                           CATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGG                  <  SRR4302536.1493543/94‑1 (MQ=60)
                                                                           CATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGG                  <  SRR4302536.3721594/94‑1 (MQ=60)
                                                                           CATAATGTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGG                  <  SRR4302536.5071755/94‑1 (MQ=60)
                                                                            ATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGG                 <  SRR4302536.1657248/94‑1 (MQ=60)
                                                                            ATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGG                 <  SRR4302536.3341013/94‑1 (MQ=60)
                                                                            ATAATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGG                 <  SRR4302536.4432166/94‑1 (MQ=60)
                                                                             TAATTTGCGGCGCACCCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGC                <  SRR4302536.1619632/94‑1 (MQ=60)
                                                                             TAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGC                <  SRR4302536.54441/94‑1 (MQ=60)
                                                                               ATTTGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGC              >  SRR4302536.6300658/1‑94 (MQ=60)
                                                                                  TGCGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGG           <  SRR4302536.3970933/94‑1 (MQ=60)
                                                                                    CGGCGCAACCGCTCAAGACAGCCGTGTATATCGCCAGAAAGCCAGATATGTCGCCGTTGATGGCCCGCAATTCTCTGATAAACGGGCGCAGGCT         <  SRR4302536.3281540/94‑1 (MQ=60)
                                                                                    CGGCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCT         >  SRR4302536.5584198/1‑94 (MQ=60)
                                                                                      GCGCAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGT       >  SRR4302536.4130531/1‑94 (MQ=60)
                                                                                         CAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTC    >  SRR4302536.1111859/1‑94 (MQ=60)
                                                                                         CAACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTC    >  SRR4302536.6405053/1‑94 (MQ=60)
                                                                                          AACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCA   >  SRR4302536.5415680/1‑94 (MQ=60)
                                                                                          AACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCA   >  SRR4302536.3613466/1‑94 (MQ=60)
                                                                                           ACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCAT  >  SRR4302536.3744910/1‑94 (MQ=60)
                                                                                           ACCGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCAT  <  SRR4302536.3687832/94‑1 (MQ=60)
                                                                                             |                                                                                           
AAACTTTGCGGCCCACGATCGATAACGTCTCCCACTGAGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCAT  >  NC_000913/1920809‑1920993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑