Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,980,940	+C	coding (1/363 nt) coding (627/627 nt)	araH ← araH ←	ABC‑type arabinose transport system permease component ABC‑type arabinose transport system permease component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,980,939	1	.	C	99.3%	449.7 / ‑3.9	142	coding (2/363 nt)	araH	ABC‑type arabinose transport system permease component
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (71/70); total (71/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 158 total aligned reads displayed.

AGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑G‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGT  >  NC_000913/1980849‑1981026

aGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑G‑cc                                                                                        >  2:2944022/1‑92 (MQ=255)

     aatTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTc                                                                                   <  2:1001401/93‑1 (MQ=255)

     aatTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTc                                                                                   >  2:1902971/1‑93 (MQ=255)

      atTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCg                                                                                  >  1:9/1‑93 (MQ=255)

      atTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCg                                                                                  >  1:3631755/1‑93 (MQ=255)

      atTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCACCAGACGCGCG‑GCC‑TCTTCg                                                                                  >  1:10/1‑93 (MQ=255)

       ttCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGt                                                                                 >  2:3516755/1‑93 (MQ=255)

         ccGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTc                                                                               <  2:4555385/93‑1 (MQ=255)

          cGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTcc                                                                              >  2:3596912/1‑93 (MQ=255)

          cGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTcc                                                                              >  1:1584759/1‑93 (MQ=255)

          cGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTcc                                                                              <  1:2430056/93‑1 (MQ=255)

           gccTATCGCTGATACAAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACATCCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTccc                                                                             <  2:2244300/91‑1 (MQ=255)

             cTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTAGACCCGCCAGAGGCGCG‑GCC‑TCTGCGTGCCCGc                                                                           >  2:3638342/1‑93 (MQ=255)

                tCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCaa                                                                        >  1:3105833/1‑93 (MQ=255)

                  gCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATc                                                                      <  1:1965630/93‑1 (MQ=255)

                  gCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑CCTTCGTTCCCGCCAATc                                                                      >  2:1868967/1‑93 (MQ=255)

                   cTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCg                                                                     >  1:1842609/1‑93 (MQ=255)

                    tGAAACCAGCCATGAGAGAACAAAGATAAGAATTTTGGTGCGAACAACCGGAACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGc                                                                    <  2:56179/93‑1 (MQ=255)

                     gATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGcc                                                                   >  1:227625/1‑93 (MQ=255)

                     gATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGcc                                                                   <  1:3045863/93‑1 (MQ=255)

                      aTACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCa                                                                  <  2:504672/93‑1 (MQ=255)

                       tACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAg                                                                 <  1:2693746/93‑1 (MQ=255)

                        aCCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAgg                                                                <  2:1823437/93‑1 (MQ=255)

                         ccAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAggg                                                               <  2:262373/93‑1 (MQ=255)

                          cAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAgggc                                                              >  1:120803/1‑92 (MQ=255)

                          cAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGgt                                                              <  2:2520770/93‑1 (MQ=255)

                           aGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCTCCAGGgtg                                                             >  2:1710323/1‑93 (MQ=255)

                           aGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGgtg                                                             <  1:4291636/93‑1 (MQ=255)

                           aGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGgtg                                                             <  1:2815597/93‑1 (MQ=255)

                            ggCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGgtgt                                                            >  1:3569267/1‑93 (MQ=255)

                            ggCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGgtgt                                                            <  2:3229968/93‑1 (MQ=255)

                            ggCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGgtgt                                                            >  1:1805322/1‑93 (MQ=255)

                            ggCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGgtgt                                                            <  2:817548/93‑1 (MQ=255)

                             gCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGtt                                                           <  1:1910767/93‑1 (MQ=255)

                              ccTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTa                                                          <  1:2590759/93‑1 (MQ=255)

                              ccTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTa                                                          <  2:4748408/93‑1 (MQ=255)

                              ccTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTa                                                          >  2:4674892/1‑93 (MQ=255)

                               cTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTAc                                                         >  1:4151167/1‑93 (MQ=255)

                                 gagagaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGa                                                       <  1:3343637/93‑1 (MQ=255)

                                   gagaAAAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGAcc                                                     <  2:11795/93‑1 (MQ=255)

                                    agaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCa                                                    >  1:1012693/1‑93 (MQ=255)

                                    agaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCa                                                    >  1:1384650/1‑93 (MQ=255)

                                     gaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCaa                                                   <  1:3742657/93‑1 (MQ=255)

                                     gaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCaa                                                   >  1:2131407/1‑93 (MQ=255)

                                      aaCAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCaaa                                                  >  2:617650/1‑93 (MQ=255)

                                       aCAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAg                                                 <  1:3580351/93‑1 (MQ=255)

                                         aaaGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggt                                               <  1:4686099/93‑1 (MQ=255)

                                         aaaGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggt                                               <  2:208052/93‑1 (MQ=255)

                                         aaaGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggg                                               >  1:4464705/1‑92 (MQ=255)

                                          aaGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtg                                              >  1:2881069/1‑93 (MQ=255)

                                           aGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtgg                                             <  1:4637003/93‑1 (MQ=255)

                                            gATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtggt                                            >  1:364987/1‑93 (MQ=255)

                                            gATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtggt                                            >  2:2139437/1‑93 (MQ=255)

                                            gATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggaggt                                            >  2:4031165/1‑93 (MQ=255)

                                            aataataATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtggt                                            <  1:341316/92‑1 (MQ=255)

                                            aataagaATTTTGTGGCGAACAACCTGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtggt                                            <  1:3210758/92‑1 (MQ=255)

                                             ataataATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGtt                                           <  1:390848/93‑1 (MQ=255)

                                              taataaTTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGttt                                          <  2:612037/93‑1 (MQ=255)

                                               aataatTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGgttt                                         >  1:1262938/1‑93 (MQ=255)

                                                ataatTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTa                                        <  1:1419936/93‑1 (MQ=255)

                                                ataatTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTa                                        <  2:3311744/93‑1 (MQ=255)

                                                 taatTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTAt                                       <  1:1361188/93‑1 (MQ=255)

                                                   atTTTGGGCCGAACACCCGGTACACCCGCCAGACGCGCG‑C‑CTTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTc                                     <  1:4020003/93‑1 (MQ=255)

                                                    ttttGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTca                                    <  1:2586319/93‑1 (MQ=255)

                                                    ttttGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTca                                    >  1:3262317/1‑93 (MQ=255)

                                                       tGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTcagca                                 <  2:1999036/93‑1 (MQ=255)

                                                              aacaacCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAAcc                          >  2:4459186/1‑93 (MQ=255)

                                                               acaacCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCa                         >  1:2041511/1‑93 (MQ=255)

                                                                 aacCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCaaa                       >  2:1459039/1‑93 (MQ=255)

                                                                  acCGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAg                      >  2:4138794/1‑93 (MQ=255)

                                                                   ccGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGa                     >  2:4084352/1‑93 (MQ=255)

                                                                    cGGTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGAt                    >  1:1592274/1‑93 (MQ=255)

                                                                     ggTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATa                   <  1:612477/93‑1 (MQ=255)

                                                                     ggTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATa                   <  2:248238/93‑1 (MQ=255)

                                                                     ggTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGCTTTATTCAGCAGCAAACCAAAGATa                   >  2:2757725/1‑93 (MQ=255)

                                                                      gTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAACCCAAAGATaa                  <  2:2498181/93‑1 (MQ=255)

                                                                      gTACACCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATaa                  >  1:2156733/1‑93 (MQ=255)

                                                                       tACCCCCGCCAGCCGCGCGAC‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAAt                 <  2:4480296/93‑1 (MQ=255)

                                                                        acacCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGTTTTTATTCAGCAGCAAACCAAAGATAATc                <  1:4141269/93‑1 (MQ=255)

                                                                         cacCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCa               >  2:4531464/1‑93 (MQ=255)

                                                                         cacCCGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCa               >  1:748284/1‑93 (MQ=255)

                                                                          ccccGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAg              <  1:2386458/92‑1 (MQ=255)

                                                                           cccGCCAGACGCGCGCC‑C‑TCTTCGTTCCCGCAAAACGCCAGGGTGTCACGACCAAAGGTGGTTTTATTCAGCAGCAATCTAAAGATAATCAGa             <  2:3635529/93‑1 (MQ=255)

                                                                           cccGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGa             >  2:1301839/1‑93 (MQ=255)

                                                                           cccGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGa             <  1:555940/93‑1 (MQ=255)

                                                                            ccGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGac            <  2:3074489/93‑1 (MQ=255)

                                                                            ccGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGac            <  2:3816237/93‑1 (MQ=255)

                                                                             cGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGaca           >  2:4362532/1‑93 (MQ=255)

                                                                             cGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGaca           >  1:1811702/1‑93 (MQ=255)

                                                                             cGCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGaca           <  1:3619900/93‑1 (MQ=255)

                                                                              gCCAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGacac          <  2:3744173/93‑1 (MQ=255)

                                                                              cccAGACGCGCG‑GCC‑TCTTCGTCCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGacac          <  1:3693486/92‑1 (MQ=255)

                                                                                cAGACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACAcgc        <  1:1659327/93‑1 (MQ=255)

                                                                                  gACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGa      >  2:4277992/1‑93 (MQ=255)

                                                                                  gACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGa      <  1:3987762/93‑1 (MQ=255)

                                                                                  gACGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGa      <  1:4457160/93‑1 (MQ=255)

                                                                                   aCGCGCG‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGAc     <  2:864664/93‑1 (MQ=255)

                                                                                     gcgcg‑GCC‑TCTTCGTGCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACgg   <  2:2352535/93‑1 (MQ=255)

                                                                                      cgcg‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGt  <  2:3986039/93‑1 (MQ=255)

                                                                                      cgcg‑GCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGt  <  2:3571834/93‑1 (MQ=255)

AGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCG‑G‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGT  >  NC_000913/1980849‑1981026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGG‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  NC_000913/1980853‑1981033

TAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTC                                                                                          <  SRR4302544.3626107/94‑1 (MQ=60)

 AATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCG                                                                                         >  SRR4302544.9/1‑94 (MQ=60)

 AATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCG                                                                                         >  SRR4302544.3667943/1‑94 (MQ=60)

 AATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCACCAGACGCGCGGCC‑TCTTCG                                                                                         >  SRR4302544.10/1‑94 (MQ=60)

     CCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCC                                                                                     >  SRR4302544.1600409/1‑94 (MQ=60)

      CGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCC                                                                                    <  SRR4302544.2453199/94‑1 (MQ=60)

         CTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCC                                                                                 >  SRR4302544.4209319/1‑94 (MQ=60)

           ATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAA                                                                               >  SRR4302544.3135394/1‑94 (MQ=60)

              GCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCG                                                                            <  SRR4302544.1984718/94‑1 (MQ=60)

              GCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCG                                                                            >  SRR4302544.1860474/1‑94 (MQ=60)

               CTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGC                                                                           >  SRR4302544.1408383/1‑94 (MQ=60)

                TGATACCAGTCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCC                                                                          <  SRR4302544.3139924/94‑1 (MQ=60)

                TGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCC                                                                          >  SRR4302544.229988/1‑94 (MQ=60)

                 GATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCA                                                                         <  SRR4302544.3074983/94‑1 (MQ=60)

                   TACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGG                                                                       <  SRR4302544.2719596/94‑1 (MQ=60)

                     CCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGC                                                                     >  SRR4302544.121902/1‑94 (MQ=60)

                      CAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTG                                                                    <  SRR4302544.4001097/94‑1 (MQ=60)

                       AGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGT                                                                   <  SRR4302544.2842774/94‑1 (MQ=60)

                       AGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGT                                                                   >  SRR4302544.1822962/1‑94 (MQ=60)

                       AGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGT                                                                   >  SRR4302544.3603492/1‑94 (MQ=60)

                       AGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGT                                                                   <  SRR4302544.4335462/94‑1 (MQ=60)

                       AGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGCGT                                                                   >  SRR4302544.3603493/1‑94 (MQ=60)

                        GGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTT                                                                  <  SRR4302544.3320277/94‑1 (MQ=60)

                         GCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTA                                                                 <  SRR4302544.1929229/94‑1 (MQ=60)

                          CCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTAC                                                                >  SRR4302544.4192271/1‑94 (MQ=60)

                          CCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTAC                                                                <  SRR4302544.2615680/94‑1 (MQ=60)

                          CCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCCGTACACCCGCCAGACGCGCGC‑CATCCTCGTTCCCGCCAATCGCCAGGGagattt                                                                >  SRR4302544.738391/1‑88 (MQ=60)

                           CTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACG                                                               >  SRR4302544.2698607/1‑94 (MQ=60)

                             GAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGAC                                                             >  SRR4302544.4068875/1‑94 (MQ=60)

                             GAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGAC                                                             <  SRR4302544.3375522/94‑1 (MQ=60)

                               GAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCA                                                           >  SRR4302544.1022787/1‑94 (MQ=60)

                               GAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCA                                                           >  SRR4302544.1022786/1‑94 (MQ=60)

                               GAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCA                                                           >  SRR4302544.1398479/1‑94 (MQ=60)

                                AGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAA                                                          >  SRR4302544.2151813/1‑94 (MQ=60)

                                 GAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAA                                                         <  SRR4302544.3779843/94‑1 (MQ=60)

                                   ACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGG                                                       >  SRR4302544.3776279/1‑94 (MQ=60)

                                   ACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGG                                                       <  SRR4302544.3614697/94‑1 (MQ=60)

                                    CAAAGATAATAATTTTTGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGG                                                      >  SRR4302544.3690513/1‑94 (MQ=60)

                                    CAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGT                                                      >  SRR4302544.2559706/1‑94 (MQ=60)

                                    CAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGG                                                      >  SRR4302544.4509630/1‑94 (MQ=60)

                                     AAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTG                                                     <  SRR4302544.4732416/94‑1 (MQ=60)

                                     AAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTG                                                     >  SRR4302544.2908865/1‑94 (MQ=60)

                                       AGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGT                                                   <  SRR4302544.2372096/94‑1 (MQ=60)

                                       AGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGT                                                   >  SRR4302544.368718/1‑94 (MQ=60)

                                       AGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGT                                                   <  SRR4302544.4682943/94‑1 (MQ=60)

                                        aataagaattttgtgGCGAACAACCTGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTT                                                  <  SRR4302544.3241381/79‑1 (MQ=60)

                                        GATAATAATTTTGGTGCGAACAACCGGTACCCCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTT                                                  <  SRR4302544.2100827/94‑1 (MQ=60)

                                        AATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTT                                                  <  SRR4302544.344848/94‑1 (MQ=60)

                                         ATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTT                                                 <  SRR4302544.394813/94‑1 (MQ=60)

                                         ATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGGGTTACGACCAAAGGTGGTTT                                                 >  SRR4302544.2871649/1‑94 (MQ=60)

                                          TAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTT                                                <  SRR4302544.1329437/94‑1 (MQ=60)

                                          TAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGGTTT                                                >  SRR4302544.1275364/1‑94 (MQ=60)

                                            ATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTAT                                              <  SRR4302544.1434072/94‑1 (MQ=60)

                                             TAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATT                                             <  SRR4302544.1374704/94‑1 (MQ=60)

                                               ATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCA                                           >  SRR4302544.3293472/1‑94 (MQ=60)

                                               ATTTTGGGCCGAACACCCGGTACACCCGCCAGACGCGCGC‑CTTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCA                                           <  SRR4302544.4060012/94‑1 (MQ=60)

                                                TTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAG                                          <  SRR4302544.2611194/94‑1 (MQ=60)

                                                TTTTGGGGCGAACAACCGGTACCCCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAG                                          <  SRR4302544.3522319/94‑1 (MQ=60)

                                                TTTGGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAG                                          <  SRR4302544.2224801/94‑1 (MQ=60)

                                                   TGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAG                                       >  SRR4302544.1560645/1‑94 (MQ=60)

                                                       GCGAACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAA                                   >  SRR4302544.1660850/1‑94 (MQ=60)

                                                          AACAACCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCA                                >  SRR4302544.2061211/1‑94 (MQ=60)

                                                               CCGGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGAT                           >  SRR4302544.1607967/1‑94 (MQ=60)

                                                                 GGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAA                         >  SRR4302544.2177229/1‑94 (MQ=60)

                                                                 GGTACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAA                         <  SRR4302544.618487/94‑1 (MQ=60)

                                                                    ACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGTTTTTATTCAGCAGCAAACCAAAGATAATCA                      <  SRR4302544.4182267/94‑1 (MQ=60)

                                                                    ACACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCA                      >  SRR4302544.755605/1‑94 (MQ=60)

                                                                     CACCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAG                     <  SRR4302544.3036247/94‑1 (MQ=60)

                                                                      CCCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGA                    <  SRR4302544.2409061/94‑1 (MQ=60)

                                                                       CCCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGAC                   <  SRR4302544.561380/94‑1 (MQ=60)

                                                                       CCCGCCAAACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGGGGTTTTATTCAGGAGCAAACCAAAAATAATCAGAC                   >  SRR4302544.1075652/1‑94 (MQ=60)

                                                                        CCGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACA                  >  SRR4302544.1829394/1‑94 (MQ=60)

                                                                         CGCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACAC                 <  SRR4302544.3655964/94‑1 (MQ=60)

                                                                          GCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCACAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACG                >  SRR4302544.1937182/1‑94 (MQ=60)

                                                                          GCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACG                <  SRR4302544.793177/94‑1 (MQ=60)

                                                                          GCCAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACG                >  SRR4302544.581839/1‑94 (MQ=60)

                                                                          CCCAGACGCGCGGCC‑TCTTCGTCCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACG                <  SRR4302544.3730283/94‑1 (MQ=60)

                                                                            CAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCG              <  SRR4302544.182382/94‑1 (MQ=60)

                                                                            CAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCG              <  SRR4302544.1675671/94‑1 (MQ=60)

                                                                            CAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCG              >  SRR4302544.192683/1‑94 (MQ=60)

                                                                            CAGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCG              >  SRR4302544.4483159/1‑94 (MQ=60)

                                                                             AGACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGA             >  SRR4302544.3656626/1‑94 (MQ=60)

                                                                              GACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGAC            <  SRR4302544.4502040/94‑1 (MQ=60)

                                                                              GACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGAC            <  SRR4302544.4027459/94‑1 (MQ=60)

                                                                               ACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACG           <  SRR4302544.4584326/94‑1 (MQ=60)

                                                                               ACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACG           >  SRR4302544.542115/1‑94 (MQ=60)

                                                                               ACGCGCGGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACG           >  SRR4302544.194760/1‑94 (MQ=60)

                                                                                      C‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGTCA   >  SRR4302544.2469012/1‑94 (MQ=60)

                                                                                      C‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCA   >  SRR4302544.2495607/1‑94 (MQ=60)

                                                                                        C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  SRR4302544.4151037/1‑94 (MQ=60)

                                                                                        C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  <  SRR4302544.4031139/94‑1 (MQ=60)

TAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGG‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  NC_000913/1980853‑1981033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`