Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,017,469	G→A	T354T (ACG→ACA)	fliK →	flagellar hook‑length control protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,017,469	0	G	A	98.4%	436.7 / ‑3.4	128	T354T (ACG→ACA)	fliK	flagellar hook‑length control protein
Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (59/67); total (59/69)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.98e-01

Only 100 of 139 total aligned reads displayed.

CTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCC  >  NC_000913/2017379‑2017558

cTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACt                                                                                         >  2:4263750/1‑93 (MQ=255)

 tttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGATGACGATACACtc                                                                                        >  2:4216992/1‑92 (MQ=255)

 tttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACtt                                                                                        >  2:1212779/1‑93 (MQ=255)

 tttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACtt                                                                                        >  1:1646615/1‑93 (MQ=255)

  ttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTc                                                                                       <  2:496101/93‑1 (MQ=255)

   tAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTcc                                                                                      <  1:480498/93‑1 (MQ=255)

   tAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTcc                                                                                      <  1:3789432/93‑1 (MQ=255)

   tAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTcc                                                                                      <  2:4581462/93‑1 (MQ=255)

    aGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATTAACCTCTGGCGGGGGAAGACGACGATACACTTCCg                                                                                     >  2:339113/1‑93 (MQ=255)

       ggTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGtt                                                                                  <  2:1274851/93‑1 (MQ=255)

          cagcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTccc                                                                               <  2:3686021/93‑1 (MQ=255)

            gcagcagGCCGCTTCCCAGCAACAGCAAATCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGt                                                                             >  1:4731432/1‑93 (MQ=255)

            gcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGt                                                                             >  1:2487941/1‑93 (MQ=255)

              agcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGtct                                                                           >  2:261875/1‑93 (MQ=255)

               gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGCACCTCTGGCGGGGGACGACGACGATCCGCTTGCGCTTCCCGtctc                                                                          >  2:2831346/1‑93 (MQ=255)

               gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGACCCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGtctc                                                                          <  2:2147342/93‑1 (MQ=255)

                 agGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCtt                                                                        >  2:70336/1‑93 (MQ=255)

                 agGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCtt                                                                        <  2:1599433/93‑1 (MQ=255)

                  ggCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCttt                                                                       >  2:1583023/1‑93 (MQ=255)

                  ggCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCttt                                                                       >  2:3123187/1‑93 (MQ=255)

                     cGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACa                                                                    >  1:1168398/1‑93 (MQ=255)

                     cGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACa                                                                    >  2:4024257/1‑93 (MQ=255)

                      gCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACaa                                                                   >  2:1488740/1‑93 (MQ=255)

                      gCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACaa                                                                   >  2:1488741/1‑93 (MQ=255)

                       cTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAg                                                                  <  2:3201949/93‑1 (MQ=255)

                       cTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAg                                                                  <  2:736911/93‑1 (MQ=255)

                         tCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAggg                                                                <  1:3993350/93‑1 (MQ=255)

                         tCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAggg                                                                >  1:557663/1‑93 (MQ=255)

                         tCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAggg                                                                <  1:1271554/93‑1 (MQ=255)

                            cagcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGTGCgt                                                             <  2:4053568/93‑1 (MQ=255)

                              gcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgtgt                                                           >  2:1819443/1‑93 (MQ=255)

                              gcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgtgt                                                           <  1:3876315/93‑1 (MQ=255)

                              gcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgtgt                                                           <  2:2570622/93‑1 (MQ=255)

                                aacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTaa                                                         <  2:3799703/93‑1 (MQ=255)

                                aacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTaa                                                         >  1:3203752/1‑93 (MQ=255)

                                aacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTaa                                                         >  1:4552281/1‑93 (MQ=255)

                                aacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTaa                                                         <  1:1012518/93‑1 (MQ=255)

                                 acagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAAc                                                        <  1:1381917/93‑1 (MQ=255)

                                    gcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAgg                                                     >  1:316285/1‑93 (MQ=255)

                                     caaAGCTAATGCACAGCAAACCATGAACCTCTAGCGGGGGAAGACGACAATAAACTTCCTGTTCCTGTGTTTTTACAAGGACGTGCAACAGGc                                                    >  2:3945442/1‑93 (MQ=255)

                                     caaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGc                                                    <  2:3892511/93‑1 (MQ=255)

                                       aaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCaa                                                  <  2:2448629/93‑1 (MQ=255)

                                        aGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAAc                                                 >  2:3893389/1‑93 (MQ=255)

                                        aGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAAc                                                 <  2:785845/93‑1 (MQ=255)

                                         gCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACa                                                <  2:4374196/93‑1 (MQ=255)

                                           cAACGCACAGCAAACCATGAGCCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgc                                              <  1:1484110/93‑1 (MQ=255)

                                           cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgc                                              <  1:28969/93‑1 (MQ=255)

                                            aaCGCACAGCAAACCATGACCCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgcg                                             <  2:3368014/93‑1 (MQ=255)

                                               gCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgcggcg                                          >  1:4463536/1‑93 (MQ=255)

                                               gCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgcggcg                                          <  2:2150161/93‑1 (MQ=255)

                                                cacaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGt                                         >  1:269521/1‑93 (MQ=255)

                                                 acaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGtt                                        >  1:1945910/1‑93 (MQ=255)

                                                   aGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGa                                      <  1:4185416/93‑1 (MQ=255)

                                                    gCAAACCATGAACCTCTGGCGGGTGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGat                                     <  1:3528981/93‑1 (MQ=255)

                                                     cAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGata                                    <  2:261350/93‑1 (MQ=255)

                                                      aaaCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGatat                                   >  1:3950463/1‑93 (MQ=255)

                                                       aaCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                  >  2:723827/1‑93 (MQ=255)

                                                       aaCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                  >  1:1530638/1‑93 (MQ=255)

                                                       aaCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                  >  2:1121396/1‑93 (MQ=255)

                                                        aCCATGAACGTCTGGCGGGGGAAGACGGCGATCCACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAttt                                 <  1:2229887/93‑1 (MQ=255)

                                                        aCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAttt                                 >  2:3782493/1‑93 (MQ=255)

                                                         ccATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtttt                                <  1:4128007/93‑1 (MQ=255)

                                                         ccATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtttt                                <  1:4319676/93‑1 (MQ=255)

                                                         ccATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtttt                                <  1:3061152/93‑1 (MQ=255)

                                                         ccATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtttt                                <  2:519907/93‑1 (MQ=255)

                                                          cATGAACCTCTGGTGGGGGAAGACGACGATACACTTCAGGTTCCCGTCGCTTTACAAGGGCGTGTAACAGGCAACAGCGGCTTTGATATTTTc                               <  2:2490189/93‑1 (MQ=255)

                                                          cATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                               >  2:1567677/1‑93 (MQ=255)

                                                          cATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                               >  1:699873/1‑93 (MQ=255)

                                                           aTGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCg                              >  1:3658885/1‑93 (MQ=255)

                                                           aTGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCg                              >  2:3593853/1‑93 (MQ=255)

                                                            tGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                             >  1:3662426/1‑93 (MQ=255)

                                                               aCCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTa                          <  1:2684490/93‑1 (MQ=255)

                                                                 ctctGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAc                        <  2:3806337/93‑1 (MQ=255)

                                                                 ctctGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAc                        <  1:4576439/93‑1 (MQ=255)

                                                                 ctctGGCGGGGGAAGACGACGATACACTTCCGGGTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAc                        <  1:4576437/93‑1 (MQ=255)

                                                                    tGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTc                     <  2:2808689/93‑1 (MQ=255)

                                                                       cGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCaga                  <  2:2442860/93‑1 (MQ=255)

                                                                       cGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCaga                  <  1:2199777/93‑1 (MQ=255)

                                                                        gggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                 >  1:4085676/1‑93 (MQ=255)

                                                                        gggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                 <  1:137903/93‑1 (MQ=255)

                                                                        gggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                 >  1:95908/1‑93 (MQ=255)

                                                                        gggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                 >  1:1461509/1‑93 (MQ=255)

                                                                        gggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                 >  1:369239/1‑93 (MQ=255)

                                                                        ggggaAAGACGATGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGTCGTTGATATTTTCGCCTAACGTCagag                 <  2:580864/93‑1 (MQ=255)

                                                                         ggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAgg                <  1:2779651/93‑1 (MQ=255)

                                                                           ggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTa              <  2:4449251/93‑1 (MQ=255)

                                                                           ggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTa              >  1:1867493/1‑93 (MQ=255)

                                                                            gAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAg             >  1:1036865/1‑93 (MQ=255)

                                                                               gacgacGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAc          <  2:3680656/93‑1 (MQ=255)

                                                                               gacgacGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAc          <  1:3074780/93‑1 (MQ=255)

                                                                                 cgacgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCg        >  1:2249298/1‑93 (MQ=255)

                                                                                 cgacgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCg        <  2:3150720/93‑1 (MQ=255)

                                                                                 cgacgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCg        <  2:1813011/93‑1 (MQ=255)

                                                                                   acgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTa      >  1:2463078/1‑93 (MQ=255)

                                                                                    cgaTACACTTCCGGCTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTaa     >  2:2659955/1‑93 (MQ=255)

                                                                                     gaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAt    >  2:2311829/1‑93 (MQ=255)

                                                                                     gaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAt    <  1:643608/93‑1 (MQ=255)

                                                                                       tACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATcc  >  1:1557372/1‑93 (MQ=255)

                                                                                       tACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATcc  <  2:1887699/93‑1 (MQ=255)

                                                                                       tACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATcc  <  1:909799/93‑1 (MQ=255)

CTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCC  >  NC_000913/2017379‑2017558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCG  >  NC_000913/2017376‑2017561

AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACA                                                                                              <  SRR4302544.3479512/94‑1 (MQ=60)

AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACA                                                                                              <  SRR4302544.4560073/94‑1 (MQ=60)

 AGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACAC                                                                                             >  SRR4302544.2276838/1‑94 (MQ=60)

 AGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACAC                                                                                             <  SRR4302544.2231336/94‑1 (MQ=60)

 AGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACAC                                                                                             >  SRR4302544.4365300/1‑94 (MQ=60)

   CTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTT                                                                                           <  SRR4302544.1411994/94‑1 (MQ=60)

   CTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTT                                                                                           >  SRR4302544.1662850/1‑94 (MQ=60)

      TAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCG                                                                                        <  SRR4302544.485203/94‑1 (MQ=60)

      TAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCG                                                                                        <  SRR4302544.3826952/94‑1 (MQ=60)

         TGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTT                                                                                     <  SRR4302544.3844933/94‑1 (MQ=60)

              AGCAGCAGGCCGCTTCCCAGCAACAGCAAATCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGT                                                                                >  SRR4302544.4778104/1‑94 (MQ=60)

              AGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGT                                                                                >  SRR4302544.2511770/1‑94 (MQ=60)

                CAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCT                                                                              <  SRR4302544.788728/94‑1 (MQ=60)

                  GCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCT                                                                            <  SRR4302544.4689548/94‑1 (MQ=60)

                      GCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTAC                                                                        >  SRR4302544.539507/1‑94 (MQ=60)

                       CCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACA                                                                       >  SRR4302544.1180050/1‑94 (MQ=60)

                        CGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAA                                                                      >  SRR4302544.3180334/1‑94 (MQ=60)

                        CGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAA                                                                      <  SRR4302544.2482165/94‑1 (MQ=60)

                           TTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGG                                                                   >  SRR4302544.3541727/1‑94 (MQ=60)

                           TTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGG                                                                   >  SRR4302544.563116/1‑94 (MQ=60)

                            TCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGC                                                                  <  SRR4302544.4033098/94‑1 (MQ=60)

                            TCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGC                                                                  <  SRR4302544.1284062/94‑1 (MQ=60)

                             CCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCG                                                                 <  SRR4302544.2161987/94‑1 (MQ=60)

                             CCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGAGCG                                                                 >  SRR4302544.2835530/1‑94 (MQ=60)

                                AGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGT                                                              >  SRR4302544.127850/1‑94 (MQ=60)

                                 GCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTA                                                             <  SRR4302544.3914804/94‑1 (MQ=60)

                                  CAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAA                                                            >  SRR4302544.3234294/1‑94 (MQ=60)

                                  CAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAA                                                            >  SRR4302544.4597722/1‑94 (MQ=60)

                                   AACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAAC                                                           <  SRR4302544.1022610/94‑1 (MQ=60)

                                    ACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACA                                                          <  SRR4302544.1395719/94‑1 (MQ=60)

                                      AGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGG                                                        >  SRR4302544.319570/1‑94 (MQ=60)

                                             CCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGC                                                 <  SRR4302544.1847145/94‑1 (MQ=60)

                                              CAACGCACAGCAAACCATGAGCCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCG                                                <  SRR4302544.1498774/94‑1 (MQ=60)

                                              CAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCG                                                <  SRR4302544.1498775/94‑1 (MQ=60)

                                              CAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCG                                                <  SRR4302544.29245/94‑1 (MQ=60)

                                                 CGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCG                                             >  SRR4302544.4508458/1‑94 (MQ=60)

                                                  GCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGT                                            >  SRR4302544.272365/1‑94 (MQ=60)

                                                   CACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTT                                           >  SRR4302544.1964752/1‑94 (MQ=60)

                                                      AGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGAT                                        <  SRR4302544.4226865/94‑1 (MQ=60)

                                                       GCAAACCATGAACCTCTGGCGGGTGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATA                                       <  SRR4302544.3562719/94‑1 (MQ=60)

                                                        CAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAT                                      >  SRR4302544.4215134/1‑94 (MQ=60)

                                                        CAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAT                                      >  SRR4302544.3989722/1‑94 (MQ=60)

                                                         AAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATT                                     >  SRR4302544.1545837/1‑94 (MQ=60)

                                                          ATCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTT                                    <  SRR4302544.3721815/94‑1 (MQ=60)

                                                           ACCATGAACGTCTGGCGGGGGAAGACGGCGATCCACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTT                                   <  SRR4302544.2250865/94‑1 (MQ=60)

                                                            CCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTC                                  <  SRR4302544.4363678/94‑1 (MQ=60)

                                                            CCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTC                                  >  SRR4302544.706844/1‑94 (MQ=60)

                                                            CCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTC                                  <  SRR4302544.4168940/94‑1 (MQ=60)

                                                            CCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTC                                  <  SRR4302544.3090361/94‑1 (MQ=60)

                                                             CATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCG                                 >  SRR4302544.3695388/1‑94 (MQ=60)

                                                              ATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGC                                >  SRR4302544.3698955/1‑94 (MQ=60)

                                                                 ATCCTCTGGCGGGGGAAGACGACGATACACTACCAGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTA                             <  SRR4302544.2552159/94‑1 (MQ=60)

                                                                  ACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAA                            <  SRR4302544.2710215/94‑1 (MQ=60)

                                                                    CTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACG                          <  SRR4302544.4622041/94‑1 (MQ=60)

                                                                    CTCTGGCGGGGGAAGACGACGATACACTTCCGGGTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACG                          <  SRR4302544.4622039/94‑1 (MQ=60)

                                                                          CGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAG                    >  SRR4302544.96789/1‑94 (MQ=60)

                                                                          CGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAG                    >  SRR4302544.4126313/1‑94 (MQ=60)

                                                                          CGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAG                    >  SRR4302544.1476007/1‑94 (MQ=60)

                                                                          CGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAG                    >  SRR4302544.373008/1‑94 (MQ=60)

                                                                          CGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAG                    <  SRR4302544.2220511/94‑1 (MQ=60)

                                                                           GGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGG                   <  SRR4302544.139206/94‑1 (MQ=60)

                                                                            GGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGT                  <  SRR4302544.2806393/94‑1 (MQ=60)

                                                                             GGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTA                 >  SRR4302544.997479/1‑94 (MQ=60)

                                                                             GGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTA                 <  SRR4302544.4508617/94‑1 (MQ=60)

                                                                             GGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTA                 >  SRR4302544.1885541/1‑94 (MQ=60)

                                                                              GGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAG                <  SRR4302544.4118450/94‑1 (MQ=60)

                                                                              GGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAG                >  SRR4302544.1047175/1‑94 (MQ=60)

                                                                                  GACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACC            <  SRR4302544.3104083/94‑1 (MQ=60)

                                                                                   ACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCG           >  SRR4302544.2270436/1‑94 (MQ=60)

                                                                                     GACGATCCACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTA         <  SRR4302544.2594389/94‑1 (MQ=60)

                                                                                     GACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTA         >  SRR4302544.2486601/1‑94 (MQ=60)

                                                                                        GATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATC      <  SRR4302544.649967/94‑1 (MQ=60)

                                                                                         ATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCC     >  SRR4302544.1572860/1‑94 (MQ=60)

                                                                                          TACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCG    <  SRR4302544.918795/94‑1 (MQ=60)

                                                                                            CACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCG  >  SRR4302544.3576702/1‑94 (MQ=60)

AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCG  >  NC_000913/2017376‑2017561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`