Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,017,469 G→A T354T (ACG→ACA)  fliK → flagellar hook‑length control protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,017,4690GA99.6% 867.8 / NA 242T354T (ACG→ACA) fliKflagellar hook‑length control protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (116/125);  total (116/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

CTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCC  >  NC_000913/2017379‑2017558
                                                                                          |                                                                                         
cTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACt                                                                                         >  1:125419/1‑93 (MQ=255)
cTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACt                                                                                         >  1:4728980/1‑93 (MQ=255)
cTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACt                                                                                         <  2:3027204/93‑1 (MQ=255)
cTTTAGTGGTCAGAAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACt                                                                                         <  2:6048094/93‑1 (MQ=255)
  ttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTc                                                                                       <  2:6559637/93‑1 (MQ=255)
    aGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTcct                                                                                     <  1:5886554/93‑2 (MQ=255)
     gTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCgg                                                                                    <  2:5757830/93‑1 (MQ=255)
     gTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCgg                                                                                    >  2:4900411/1‑93 (MQ=255)
      tGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGt                                                                                   >  1:3021575/1‑93 (MQ=255)
        gTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTc                                                                                 >  1:4837683/1‑93 (MQ=255)
         tCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTcc                                                                                >  1:2609990/1‑93 (MQ=255)
          cagcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTccc                                                                               >  1:4187170/1‑93 (MQ=255)
          cagcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTccc                                                                               <  1:921451/93‑1 (MQ=255)
            gcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGCAGACGACGATACACTTCCGGCTCCCGt                                                                             >  2:6180459/1‑93 (MQ=255)
            gcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGt                                                                             >  1:6500874/1‑93 (MQ=255)
             cagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGtc                                                                            <  2:727364/93‑1 (MQ=255)
               gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGtctc                                                                          <  2:2084629/93‑1 (MQ=255)
               gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGtctc                                                                          >  2:946078/1‑93 (MQ=255)
                cagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTctct                                                                         <  1:4434427/93‑1 (MQ=255)
                cagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTctct                                                                         <  2:167027/93‑1 (MQ=255)
                cagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTctct                                                                         <  1:6704518/93‑1 (MQ=255)
                 agGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCtt                                                                        <  1:5379970/93‑1 (MQ=255)
                  ggCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGCCGGCGATACACTTCCGGATCCCGTCTCttt                                                                       >  1:3891036/1‑93 (MQ=255)
                  ggCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCttt                                                                       >  1:1307985/1‑93 (MQ=255)
                   gCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTa                                                                      >  2:2832291/1‑93 (MQ=255)
                    ccGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTAc                                                                     <  1:4611803/93‑1 (MQ=255)
                     cGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACa                                                                    >  2:596918/1‑93 (MQ=255)
                      gCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACaa                                                                   >  2:468580/1‑93 (MQ=255)
                       cTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACTATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAg                                                                  <  1:4945404/93‑1 (MQ=255)
                       cTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAg                                                                  >  2:6387132/1‑93 (MQ=255)
                       cTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAg                                                                  <  1:691870/93‑1 (MQ=255)
                        ttCCCAGCAACAGCAAAGCCTACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAgg                                                                 <  2:4437079/93‑1 (MQ=255)
                        ttCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAgg                                                                 >  2:1635797/1‑93 (MQ=255)
                           ccAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCg                                                              <  1:1786959/93‑1 (MQ=255)
                             agcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgtg                                                            <  2:2175808/93‑1 (MQ=255)
                               caacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTa                                                          >  2:5244513/1‑93 (MQ=255)
                                   agcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAg                                                      >  2:872462/1‑93 (MQ=255)
                                   agcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAg                                                      >  2:5540387/1‑93 (MQ=255)
                                   agcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAg                                                      <  1:5060663/93‑1 (MQ=255)
                                    gcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAgg                                                     <  2:645531/93‑1 (MQ=255)
                                    gcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAAACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAgg                                                     >  1:6625878/1‑93 (MQ=255)
                                      aaaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCa                                                   <  2:30662/93‑1 (MQ=255)
                                      aaaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCa                                                   >  2:1977740/1‑93 (MQ=255)
                                       aaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTGTTTACAAGGGCGTGTAACAGGCaa                                                  <  1:6465481/93‑1 (MQ=255)
                                       aaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCaa                                                  >  2:1359019/1‑93 (MQ=255)
                                       aaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCaa                                                  >  2:831301/1‑93 (MQ=255)
                                       aaGCCAACGCACAACAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCaa                                                  <  2:1152499/93‑1 (MQ=255)
                                        aGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAAc                                                 <  1:4865995/93‑1 (MQ=255)
                                         gCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACa                                                <  2:3326161/93‑1 (MQ=255)
                                           cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgc                                              <  1:5962630/93‑1 (MQ=255)
                                           cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgc                                              <  1:3558274/93‑1 (MQ=255)
                                             aCGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgcgg                                            >  2:5835524/1‑93 (MQ=255)
                                              cGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgcggc                                           <  2:4232887/93‑1 (MQ=255)
                                              cGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgcggc                                           >  1:2326325/1‑93 (MQ=255)
                                               gCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgcggcg                                          >  1:3179745/1‑93 (MQ=255)
                                                cacaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGt                                         <  2:1580606/93‑1 (MQ=255)
                                                 acaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGtt                                        >  2:1878942/1‑93 (MQ=255)
                                                   aGCAACCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGa                                      <  1:2823720/93‑1 (MQ=255)
                                                    gCAAACCATGAACCTCGGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGat                                     <  2:2336922/93‑1 (MQ=255)
                                                     cAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGata                                    >  2:4751625/1‑93 (MQ=255)
                                                     cAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGata                                    <  2:35642/93‑1 (MQ=255)
                                                      aaaCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGatat                                   <  2:4828838/93‑1 (MQ=255)
                                                      aaaCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGatat                                   <  1:2664424/93‑1 (MQ=255)
                                                      aaaCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGatat                                   >  2:5114764/1‑93 (MQ=255)
                                                        aCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAttt                                 <  2:3093536/93‑1 (MQ=255)
                                                         ccATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtttt                                <  2:3429521/93‑1 (MQ=255)
                                                          cATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                               >  2:3146371/1‑93 (MQ=255)
                                                          cATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                               <  2:3881127/93‑1 (MQ=255)
                                                            tGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                             >  1:6192413/1‑93 (MQ=255)
                                                             gAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGcc                            <  1:1890631/93‑1 (MQ=255)
                                                             gAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGcc                            >  2:2551602/1‑93 (MQ=255)
                                                              aaCCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCt                           <  1:3682449/93‑1 (MQ=255)
                                                              aaCCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCt                           <  2:2632290/93‑1 (MQ=255)
                                                               aCCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTa                          <  1:62339/93‑1 (MQ=255)
                                                                 ctctGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAc                        <  2:3539822/93‑1 (MQ=255)
                                                                 ctctGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAc                        >  2:5427838/1‑93 (MQ=255)
                                                                 ctctGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAc                        <  1:4351161/93‑1 (MQ=255)
                                                                     ggCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCa                    >  2:2431189/1‑93 (MQ=255)
                                                                      gggggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCag                   <  1:2402163/91‑1 (MQ=255)
                                                                        gggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                 <  2:1536889/93‑1 (MQ=255)
                                                                         ggggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAgg                >  1:380704/1‑93 (MQ=255)
                                                                         ggggAAGACGACGATACACTTCCGGTTCCCGCCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAGCGTCAGAgg                >  2:3292785/1‑93 (MQ=255)
                                                                          gggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGt               >  2:69481/1‑93 (MQ=255)
                                                                          gggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGt               <  2:3804342/93‑1 (MQ=255)
                                                                            gAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAg             <  1:5680539/93‑1 (MQ=255)
                                                                             aaGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGc            <  2:2866743/93‑1 (MQ=255)
                                                                              aGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCa           >  1:2875329/1‑93 (MQ=255)
                                                                              aGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCa           >  1:1625745/1‑93 (MQ=255)
                                                                               gacgacGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAc          >  2:1049696/1‑93 (MQ=255)
                                                                               gacgacGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAc          >  2:5901378/1‑93 (MQ=255)
                                                                                  gacgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGt       >  2:6598016/1‑93 (MQ=255)
                                                                                  gacgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGt       >  1:1516823/1‑93 (MQ=255)
                                                                                   acgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTa      >  1:5149486/1‑93 (MQ=255)
                                                                                   acgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCCAACGTCAGAGGTAGCACCGTa      <  1:3101866/93‑1 (MQ=255)
                                                                                    cgaTACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTaa     <  2:4377943/93‑1 (MQ=255)
                                                                                    cgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTaa     >  2:4294377/1‑93 (MQ=255)
                                                                                      aTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAta   >  2:6434383/1‑92 (MQ=255)
                                                                                      aTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATc   <  2:6292740/93‑1 (MQ=255)
                                                                                      aTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATc   <  2:1810581/93‑1 (MQ=255)
                                                                                       tACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATcc  >  2:4106330/1‑93 (MQ=255)
                                                                                          |                                                                                         
CTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCC  >  NC_000913/2017379‑2017558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCG  >  NC_000913/2017376‑2017561
                                                                                             |                                                                                            
AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACA                                                                                              >  SRR4302536.1670138/1‑94 (MQ=60)
  GCTTTAGTGGTCAGCAGCAGGCTGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACT                                                                                            <  SRR4302536.3141783/94‑1 (MQ=60)
  GCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACT                                                                                            >  SRR4302536.128729/1‑94 (MQ=60)
  GCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACT                                                                                            <  SRR4302536.2420461/94‑1 (MQ=60)
  GCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACT                                                                                            <  SRR4302536.4372417/94‑1 (MQ=60)
  GCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACT                                                                                            >  SRR4302536.4871269/1‑94 (MQ=60)
   CTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTT                                                                                           >  SRR4302536.5264569/1‑94 (MQ=60)
   CTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTT                                                                                           >  SRR4302536.2797611/1‑94 (MQ=60)
    TTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTC                                                                                          <  SRR4302536.3356676/94‑1 (MQ=60)
      TAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCG                                                                                        >  SRR4302536.5211010/1‑94 (MQ=60)
      TAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCG                                                                                        <  SRR4302536.2512757/94‑1 (MQ=60)
       AGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCTG                                                                                       <  SRR4302536.6061872/94‑1 (MQ=60)
        GTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGT                                                                                      >  SRR4302536.3110355/1‑94 (MQ=60)
         TGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTT                                                                                     >  SRR4302536.316196/1‑94 (MQ=60)
          GGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTC                                                                                    >  SRR4302536.4983289/1‑94 (MQ=60)
           GTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCC                                                                                   >  SRR4302536.2688308/1‑94 (MQ=60)
            TCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCC                                                                                  >  SRR4302536.4311589/1‑94 (MQ=60)
             CAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCG                                                                                 <  SRR4302536.948606/94‑1 (MQ=60)
              AGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGT                                                                                >  SRR4302536.6694631/1‑94 (MQ=60)
                CAGCAGGCCGCTTCCCAGCAACAGCAAAGCCANCGNACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCT                                                                              <  SRR4302536.5260588/94‑1 (MQ=60)
                CAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCT                                                                              >  SRR4302536.5908732/1‑94 (MQ=60)
                CAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTACGCTTCCCGTCT                                                                              >  SRR4302536.4528561/1‑94 (MQ=60)
                CACCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAGCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCT                                                                              <  SRR4302536.5789275/94‑1 (MQ=60)
                 AGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGCTACACTTCCGGTTCCCGTCTC                                                                             >  SRR4302536.269531/1‑94 (MQ=60)
                 AGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTGCCGGTTCCCGTCTC                                                                             >  SRR4302536.2919220/1‑94 (MQ=60)
                  GCAGTCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCT                                                                            <  SRR4302536.6833132/94‑1 (MQ=60)
                   CAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTT                                                                           <  SRR4302536.4567441/94‑1 (MQ=60)
                   CAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTT                                                                           <  SRR4302536.6903624/94‑1 (MQ=60)
                    AGGCCGCTTCCCAGCAACAGCAAAGCCAACGCAGAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGATTACGGTTCCCGTCTCTTT                                                                          >  SRR4302536.2993997/1‑94 (MQ=60)
                    AGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGCCGGCGATACACTTCCGGATCCCGTCTCTTT                                                                          >  SRR4302536.4006736/1‑94 (MQ=60)
                    AGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTT                                                                          >  SRR4302536.1347032/1‑94 (MQ=60)
                    AGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTT                                                                          <  SRR4302536.5540506/94‑1 (MQ=60)
                    AGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTT                                                                          >  SRR4302536.4912274/1‑94 (MQ=60)
                    AGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATAAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGGTCCCGTCTCTTT                                                                          >  SRR4302536.3203881/1‑94 (MQ=60)
                     GGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTA                                                                         >  SRR4302536.6662258/1‑94 (MQ=60)
                     GGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTA                                                                         <  SRR4302536.21522/94‑1 (MQ=60)
                       CCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACA                                                                       >  SRR4302536.2429121/1‑94 (MQ=60)
                       CCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACA                                                                       <  SRR4302536.4750338/94‑1 (MQ=60)
                          CTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACTATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGG                                                                    <  SRR4302536.5094023/94‑1 (MQ=60)
                          CTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGG                                                                    <  SRR4302536.6455676/94‑1 (MQ=60)
                          CTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGG                                                                    <  SRR4302536.712093/94‑1 (MQ=60)
                          CTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGGTTCCGTCTCTTTACAAAG                                                                    >  SRR4302536.489056/1‑94 (MQ=60)
                           TTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGG                                                                   <  SRR4302536.3311567/94‑1 (MQ=60)
                            TCACAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGC                                                                  <  SRR4302536.1392379/94‑1 (MQ=60)
                              CCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGT                                                                <  SRR4302536.1840957/94‑1 (MQ=60)
                                AGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGT                                                              <  SRR4302536.819572/94‑1 (MQ=60)
                                AGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGT                                                              <  SRR4302536.5856636/94‑1 (MQ=60)
                                AGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGT                                                              <  SRR4302536.4812349/94‑1 (MQ=60)
                                AGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGT                                                              >  SRR4302536.1862443/1‑94 (MQ=60)
                                AGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGT                                                              <  SRR4302536.2927180/94‑1 (MQ=60)
                                 GCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTA                                                             <  SRR4302536.6143632/94‑1 (MQ=60)
                                    acatctaacccacCGCACAGCCAACCATGAACCTGTGCCGGGGGAAGACGACGATACGCTGCCGTTTCGCGTCTCTTTACAAGGGCGagtgacg                                                          <  SRR4302536.1945912/81‑8 (MQ=52)
                                    ACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACA                                                          <  SRR4302536.4633401/94‑1 (MQ=60)
                                     CAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAG                                                         <  SRR4302536.5382507/94‑1 (MQ=60)
                                     CAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAG                                                         <  SRR4302536.4672128/94‑1 (MQ=60)
                                      AGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGG                                                        <  SRR4302536.5212248/94‑1 (MQ=60)
                                      AGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAAACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGG                                                        >  SRR4302536.6822954/1‑94 (MQ=60)
                                        CAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGTAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCA                                                      <  SRR4302536.4261981/94‑1 (MQ=60)
                                         AAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAA                                                     >  SRR4302536.6723859/1‑94 (MQ=60)
                                          AAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTGTTTACAAGGGCGTGTAACAGGCAAC                                                    <  SRR4302536.6658464/94‑1 (MQ=60)
                                           AGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACA                                                   <  SRR4302536.5012426/94‑1 (MQ=60)
                                              CAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCG                                                <  SRR4302536.6140339/94‑1 (MQ=60)
                                              CAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCG                                                <  SRR4302536.3664677/94‑1 (MQ=60)
                                              CAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCG                                                >  SRR4302536.6137411/1‑94 (MQ=60)
                                                ACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGC                                              >  SRR4302536.2396932/1‑94 (MQ=60)
                                                 CGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCG                                             >  SRR4302536.3272977/1‑94 (MQ=60)
                                                 CGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCG                                             <  SRR4302536.405058/94‑1 (MQ=60)
                                                 CGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCG                                             <  SRR4302536.1654318/94‑1 (MQ=60)
                                                    ACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTG                                          <  SRR4302536.4422856/94‑1 (MQ=60)
                                                    ACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTG                                          <  SRR4302536.13543/94‑1 (MQ=60)
                                                     CAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGA                                         >  SRR4302536.5585410/1‑94 (MQ=60)
                                                     CAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCCTTACACGGGGGTGTAACAGGCAAGCGAGGCGTTGA                                         >  SRR4302536.37792/1‑94 (MQ=60)
                                                      AGCAACCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGAT                                        <  SRR4302536.2907336/94‑1 (MQ=60)
                                                      AGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGAT                                        >  SRR4302536.2094264/1‑94 (MQ=60)
                                                      AGCAAACCATGAACCTCTGGCGGGGGAAGACCACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGAT                                        <  SRR4302536.5035732/94‑1 (MQ=60)
                                                       GCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATA                                       >  SRR4302536.1722722/1‑94 (MQ=60)
                                                       GAAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATA                                       <  SRR4302536.3899762/94‑1 (MQ=60)
                                                         AAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATT                                     <  SRR4302536.2744147/94‑1 (MQ=60)
                                                          AACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTT                                    >  SRR4302536.1335445/1‑94 (MQ=60)
                                                              ATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGC                                >  SRR4302536.6376872/1‑94 (MQ=60)
                                                                GAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCT                              <  SRR4302536.1947494/94‑1 (MQ=60)
                                                                 caccgCTGGCGGGGGAAGACGACGATCCGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGCAACAGGCACCAGCGGCGTTGATATTTTCGCCTA                             <  SRR4302536.2816397/89‑1 (MQ=60)
                                                                 AACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTA                             <  SRR4302536.3792536/94‑1 (MQ=60)
                                                                  ACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAA                            <  SRR4302536.64047/94‑1 (MQ=60)
                                                                    CTCTGTCGGTGCAAGACGACGCTACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACG                          <  SRR4302536.6391692/94‑1 (MQ=60)
                                                                    CTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACG                          <  SRR4302536.4481344/94‑1 (MQ=60)
                                                                        GGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAG                      <  SRR4302536.4984954/94‑1 (MQ=60)
                                                                        GGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAG                      <  SRR4302536.75839/94‑1 (MQ=60)
                                                                         GGGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGA                     <  SRR4302536.2474854/94‑1 (MQ=60)
                                                                           GGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGG                   <  SRR4302536.6620160/94‑1 (MQ=60)
                                                                           GGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGG                   >  SRR4302536.390728/1‑94 (MQ=60)
                                                                           GGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGAGATTTTCGCCTAACGACAGAGG                   >  SRR4302536.3855092/1‑94 (MQ=60)
                                                                            GGGGAAGACGACGATACACTCCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGT                  >  SRR4302536.6735546/1‑94 (MQ=60)
                                                                             GGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTA                 <  SRR4302536.2517819/94‑1 (MQ=60)
                                                                             GGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTA                 <  SRR4302536.3984571/94‑1 (MQ=60)
                                                                              GTAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAG                <  SRR4302536.4435115/94‑1 (MQ=60)
                                                                               GAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGC               <  SRR4302536.5849431/94‑1 (MQ=60)
                                                                                AAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCA              >  SRR4302536.2960220/1‑94 (MQ=60)
                                                                                AAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCA              >  SRR4302536.6918277/1‑94 (MQ=60)
                                                                                AAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCA              >  SRR4302536.1673077/1‑94 (MQ=60)
                                                                                 AGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAC             >  SRR4302536.69952/1‑94 (MQ=60)
                                                                                   ACGACGATACACTCCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCG           <  SRR4302536.4285724/94‑1 (MQ=60)
                                                                                    CGACGATACACTTCCGGTTCCCGTCTCTTTACAATGGCATGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGGAGCACCGT          <  SRR4302536.1827405/94‑1 (MQ=60)
                                                                                    CGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGT          >  SRR4302536.1561479/1‑94 (MQ=60)
                                                                                    CGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGT          >  SRR4302536.3140602/1‑94 (MQ=60)
                                                                                     GACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTA         >  SRR4302536.5303142/1‑94 (MQ=60)
                                                                                      ACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCCAACGTCAGAGGTAGCACCGTAA        <  SRR4302536.3192414/94‑1 (MQ=60)
                                                                                       CGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAT       >  SRR4302536.6169643/1‑94 (MQ=60)
                                                                                        GATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATC      <  SRR4302536.503285/94‑1 (MQ=60)
                                                                                           ACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGC   <  SRR4302536.2580437/94‑1 (MQ=60)
                                                                                            CACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCG  <  SRR4302536.3691731/94‑1 (MQ=60)
                                                                                            CACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCG  <  SRR4302536.4948865/94‑1 (MQ=60)
                                                                                             |                                                                                            
AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCG  >  NC_000913/2017376‑2017561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑