Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,028,708 A→G V78A (GTG→GCG)  vsr ← DNA mismatch endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,028,7080AG98.4% 399.9 / ‑2.7 127V78A (GTG→GCG) vsrDNA mismatch endonuclease
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (73/52);  total (74/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 130 total aligned reads displayed.

CCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCACTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACG  >  NC_000913/2028618‑2028796
                                                                                          |                                                                                        
ccGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCt                                                                                        <  1:1843202/93‑1 (MQ=255)
  gAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCttt                                                                                      <  2:410850/93‑1 (MQ=255)
    gTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGTCAGAACTCGGTTCGAGTCGCAGGCGCTTTaa                                                                                    >  1:1385800/1‑93 (MQ=255)
     ttCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTaaa                                                                                   >  1:759505/1‑93 (MQ=255)
         tGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGa                                                                               <  2:1230999/93‑1 (MQ=255)
         tGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGa                                                                               >  1:712936/1‑93 (MQ=255)
         tGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGa                                                                               >  2:1454533/1‑93 (MQ=255)
          gCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGAt                                                                              <  2:1011034/93‑1 (MQ=255)
          gCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGAt                                                                              <  2:3572417/93‑1 (MQ=255)
          gCAAGCGACTGATATCGCGGCGATCGCGCCCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGAt                                                                              >  1:4542886/1‑93 (MQ=255)
          gCAAGCGACTGATATCGCGGCGAACGCGCTCAAAATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGAt                                                                              <  1:4195260/93‑1 (MQ=255)
           cAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATa                                                                             >  1:3113857/1‑93 (MQ=255)
           cAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATa                                                                             <  2:248007/93‑1 (MQ=255)
           cAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATa                                                                             >  1:4399501/1‑93 (MQ=255)
              gCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCa                                                                          <  2:1328199/93‑1 (MQ=255)
               cGACTGATATCGCGTCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCTGTTCGAGTCGCAGGCGCGTTAAACAGATAGCAg                                                                         <  1:2924499/93‑1 (MQ=255)
                gACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGt                                                                        >  1:1908103/1‑93 (MQ=255)
                 aCTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtg                                                                       >  1:2285225/1‑93 (MQ=255)
                  cTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtga                                                                      >  1:4274728/1‑93 (MQ=255)
                  cTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtga                                                                      >  1:1332641/1‑93 (MQ=255)
                   tGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgat                                                                     <  2:2743334/93‑1 (MQ=255)
                   tGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGggat                                                                     >  1:1250717/1‑93 (MQ=255)
                   tGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAAAAGATAGCAGtgat                                                                     <  1:4537363/93‑1 (MQ=255)
                      tatCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgatgat                                                                  >  2:4745299/1‑93 (MQ=255)
                      tatCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgatgat                                                                  >  2:427013/1‑93 (MQ=255)
                      tatCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgatgat                                                                  >  1:2907473/1‑93 (MQ=255)
                        tCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGtgatgatga                                                                <  2:3504942/93‑1 (MQ=255)
                          gcggcgATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGatgatgatg                                                              >  2:2468574/1‑93 (MQ=255)
                          gcggcgATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGatgatgatg                                                              >  1:4254761/1‑93 (MQ=255)
                            ggcgATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGcc                                                            <  1:3316249/93‑1 (MQ=255)
                              cgATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAg                                                          >  1:2243172/1‑93 (MQ=255)
                                atggcgcTCAACATATTTACCTATCTGCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGaa                                                        <  1:1927222/90‑1 (MQ=255)
                                aTCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGaa                                                        >  2:488498/1‑93 (MQ=255)
                                 tCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAag                                                       >  1:2928492/1‑93 (MQ=255)
                                 tCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAag                                                       >  2:62373/1‑93 (MQ=255)
                                  cgcgCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAagc                                                      <  1:3163138/93‑1 (MQ=255)
                                   gcgcTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAagca                                                     >  1:3327822/1‑93 (MQ=255)
                                   gcgcTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAagca                                                     >  2:393669/1‑93 (MQ=255)
                                    cgcTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAagcag                                                    >  2:665320/1‑93 (MQ=255)
                                    cgcTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAagcag                                                    >  1:3842704/1‑93 (MQ=255)
                                     gcTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAagcagc                                                   <  1:1012447/93‑1 (MQ=255)
                                       tCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCa                                                 <  1:1979066/93‑1 (MQ=255)
                                        cAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAt                                                <  1:1626491/93‑1 (MQ=255)
                                        cAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAt                                                <  2:1871559/93‑1 (MQ=255)
                                        cAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAt                                                <  2:1871560/93‑1 (MQ=255)
                                        cAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAt                                                <  1:3138268/93‑1 (MQ=255)
                                        cAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAt                                                <  2:3136936/93‑1 (MQ=255)
                                         aaCATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATg                                               >  2:1477892/1‑93 (MQ=255)
                                         aaCATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATg                                               >  1:2022812/1‑93 (MQ=255)
                                          aCATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATgg                                              >  1:1700356/1‑93 (MQ=255)
                                          aCATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATgg                                              <  1:2559202/93‑1 (MQ=255)
                                          aCATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATgg                                              <  1:3271185/93‑1 (MQ=255)
                                          aCATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATCCCAGAAGCAGCCATgg                                              >  2:3880327/1‑93 (MQ=255)
                                           cATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATggg                                             >  1:1169358/1‑93 (MQ=255)
                                               tttACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTaaa                                         >  1:282751/1‑93 (MQ=255)
                                                 tACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAata                                       >  1:821526/1‑93 (MQ=255)
                                                   ccTATCTTCTCCAGGCAGAACTCGGTTCGAGACGCACGCGCGTTAAACAGAGAGCAGTTATGATGATGCCAGAAGCAGCCATGGGTAAATATc                                     >  2:2623276/1‑93 (MQ=255)
                                                    cTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCa                                    <  1:352616/93‑1 (MQ=255)
                                                    cTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCa                                    >  2:1530582/1‑93 (MQ=255)
                                                    cTACCTGCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCa                                    <  2:3186258/93‑1 (MQ=255)
                                                     tATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGGAAATATAAc                                   >  2:38014/1‑93 (MQ=255)
                                                     tATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCACTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTCAATATCAc                                   >  2:3306374/1‑93 (MQ=255)
                                                       tcttctCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGc                                 <  2:128939/93‑1 (MQ=255)
                                                       tcttctCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGc                                 <  1:2060737/93‑1 (MQ=255)
                                                         ttctCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAg                               >  2:4206449/1‑93 (MQ=255)
                                                           ctcCAGCCAGATCTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCg                             <  2:693987/93‑1 (MQ=255)
                                                           ctcCAGCCAGACCTCGGTTCGAGTCGCAGGCACTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCg                             <  2:3698624/93‑1 (MQ=255)
                                                            tcCAGGCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGa                            >  2:3957964/1‑93 (MQ=255)
                                                            tcCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGa                            <  2:1860321/93‑1 (MQ=255)
                                                            tcCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGa                            >  2:2507683/1‑93 (MQ=255)
                                                            cccagccagAACTCGGTTTGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGa                            <  1:2300610/92‑1 (MQ=255)
                                                              cagccagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGata                          >  2:4111605/1‑93 (MQ=255)
                                                              cagccagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGata                          >  1:1666945/1‑93 (MQ=255)
                                                              cagccagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGata                          >  2:1029388/1‑93 (MQ=255)
                                                              cagccagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGata                          <  1:3349269/93‑1 (MQ=255)
                                                                gctagAACTCGGTTCGTGTCGCAGGCGCTTTAAACAGATAGCAGTTATGATGATGCCAGAAGCAGCCATGGGGTATTATCACGCAGCGATAtt                        >  2:4216675/4‑93 (MQ=255)
                                                                 ccagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtc                       >  2:2634784/1‑93 (MQ=255)
                                                                 ccagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtc                       <  1:1434676/93‑1 (MQ=255)
                                                                  cagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtca                      >  2:2958169/1‑93 (MQ=255)
                                                                  cagAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtca                      >  1:3098294/1‑93 (MQ=255)
                                                                   agAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtcat                     >  2:1264186/1‑93 (MQ=255)
                                                                   agAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtcat                     >  1:3750338/1‑93 (MQ=255)
                                                                    gAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATtcatc                    >  1:2552349/1‑93 (MQ=255)
                                                                      aCTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCaa                  >  2:4161764/1‑93 (MQ=255)
                                                                      aCTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCaa                  <  1:3103963/93‑1 (MQ=255)
                                                                      aCTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCaa                  <  2:2964326/93‑1 (MQ=255)
                                                                       cTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAAc                 <  1:1532300/93‑1 (MQ=255)
                                                                        tCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACg                >  1:2111887/1‑93 (MQ=255)
                                                                         cGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGa               >  2:1421091/1‑93 (MQ=255)
                                                                          ggTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGAc              >  2:4397041/1‑93 (MQ=255)
                                                                           gTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACa             >  1:83064/1‑93 (MQ=255)
                                                                               gAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAAt         >  2:4100591/1‑93 (MQ=255)
                                                                                  tCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCg      <  1:4162240/93‑1 (MQ=255)
                                                                                  tCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCg      <  1:4052066/93‑1 (MQ=255)
                                                                                  tCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCg      <  1:4052064/93‑1 (MQ=255)
                                                                                  tCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCg      <  1:2413672/93‑1 (MQ=255)
                                                                                  tCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCg      <  1:3670119/93‑1 (MQ=255)
                                                                                    gCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCggc    >  1:4713782/1‑92 (MQ=255)
                                                                                     cAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGAc   >  2:2072172/1‑93 (MQ=255)
                                                                                      aGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACg  <  2:2180145/93‑1 (MQ=255)
                                                                                          |                                                                                        
CCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCACTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACG  >  NC_000913/2028618‑2028796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCACTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTCCG  >  NC_000913/2028615‑2028800
                                                                                             |                                                                                            
CAGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCG                                                                                              <  SRR4302544.3662153/94‑1 (MQ=60)
CAGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCG                                                                                              <  SRR4302544.56753/94‑1 (MQ=60)
CAGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCG                                                                                              >  SRR4302544.1370395/1‑94 (MQ=60)
CAGCCGAGTTCCTGCAAGCGACTGATAGCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCG                                                                                              <  SRR4302544.56754/94‑1 (MQ=60)
  GCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCT                                                                                            <  SRR4302544.1181404/94‑1 (MQ=60)
  GCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCT                                                                                            <  SRR4302544.1181545/94‑1 (MQ=60)
  GCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCT                                                                                            <  SRR4302544.1757335/94‑1 (MQ=60)
   CCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTT                                                                                           <  SRR4302544.1861069/94‑1 (MQ=60)
     GAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTA                                                                                         >  SRR4302544.1775357/1‑94 (MQ=60)
      AGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGTCAGAACTCGGTTCGAGTCGCAGGCGCTTTAA                                                                                        >  SRR4302544.1399636/1‑94 (MQ=60)
       GTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAA                                                                                       >  SRR4302544.766914/1‑94 (MQ=60)
           CTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGA                                                                                   >  SRR4302544.719998/1‑94 (MQ=60)
            TGCAAGCGACTGATATCGCGGCGATCGCGCCCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGAT                                                                                  >  SRR4302544.4588278/1‑94 (MQ=60)
             GCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATA                                                                                 >  SRR4302544.4443968/1‑94 (MQ=60)
             GCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATA                                                                                 >  SRR4302544.3143478/1‑94 (MQ=60)
             GCAAGCGACTGATATCGCGGCGAACGCGCTCAAAATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATA                                                                                 <  SRR4302544.4236896/94‑1 (MQ=60)
                  CGACTGATATCGCGTCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCTGTTCGAGTCGCAGGCGCGTTAAACAGATAGCAGT                                                                            <  SRR4302544.2952608/94‑1 (MQ=60)
                  CGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGT                                                                            >  SRR4302544.1926550/1‑94 (MQ=60)
                  CGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGT                                                                            >  SRR4302544.1389050/1‑94 (MQ=60)
                   GACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTG                                                                           >  SRR4302544.698091/1‑94 (MQ=60)
                   GACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTG                                                                           >  SRR4302544.2306767/1‑94 (MQ=60)
                    ACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGA                                                                          >  SRR4302544.1345762/1‑94 (MQ=60)
                    ACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGA                                                                          >  SRR4302544.4318371/1‑94 (MQ=60)
                     CTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGGGAT                                                                         >  SRR4302544.1263027/1‑94 (MQ=60)
                      TGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAAAAGATAGCAGTGATG                                                                        <  SRR4302544.4582727/94‑1 (MQ=60)
                        ATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGAT                                                                      >  SRR4302544.2935454/1‑94 (MQ=60)
                        ATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGAA                                                                      <  SRR4302544.4240575/94‑1 (MQ=60)
                            CGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATG                                                                  >  SRR4302544.4298138/1‑94 (MQ=60)
                            CGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATG                                                                  <  SRR4302544.3608787/94‑1 (MQ=60)
                               GGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCA                                                               <  SRR4302544.3347841/94‑1 (MQ=60)
                               GGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCA                                                               >  SRR4302544.3151130/1‑94 (MQ=60)
                                GCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAG                                                              >  SRR4302544.2264272/1‑94 (MQ=60)
                                   ATGGCGCTCAACATATTTACCTATCTGCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAG                                                           <  SRR4302544.1945871/94‑1 (MQ=60)
                                   ATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAG                                                           >  SRR4302544.2956633/1‑94 (MQ=60)
                                     CGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCA                                                         >  SRR4302544.3359512/1‑94 (MQ=60)
                                     CGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCA                                                         <  SRR4302544.3193228/94‑1 (MQ=60)
                                     CGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCA                                                         >  SRR4302544.3359513/1‑94 (MQ=60)
                                     CGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCA                                                         <  SRR4302544.4003295/94‑1 (MQ=60)
                                      GCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAG                                                        >  SRR4302544.3880827/1‑94 (MQ=60)
                                        GCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCC                                                      <  SRR4302544.1022539/94‑1 (MQ=60)
                                          TCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAT                                                    <  SRR4302544.1998294/94‑1 (MQ=60)
                                          TCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCAT                                                    <  SRR4302544.1998293/94‑1 (MQ=60)
                                           CAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATG                                                   >  SRR4302544.2042436/1‑94 (MQ=60)
                                           CAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATG                                                   <  SRR4302544.1642515/94‑1 (MQ=60)
                                           CAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATG                                                   <  SRR4302544.3168089/94‑1 (MQ=60)
                                            AACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGG                                                  >  SRR4302544.3118795/1‑94 (MQ=60)
                                            AACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGG                                                  >  SRR4302544.1717135/1‑94 (MQ=60)
                                             ACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGG                                                 <  SRR4302544.2583782/94‑1 (MQ=60)
                                             ACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGG                                                 >  SRR4302544.1181020/1‑94 (MQ=60)
                                             ACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGG                                                 <  SRR4302544.3302385/94‑1 (MQ=60)
                                              CATTTTTACCTATCTTCTCCAGCCAGATCTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGT                                                <  SRR4302544.2896086/94‑1 (MQ=60)
                                                 TTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAA                                             >  SRR4302544.285701/1‑94 (MQ=60)
                                                   TTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATA                                           >  SRR4302544.829558/1‑94 (MQ=60)
                                                       CTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCAC                                       <  SRR4302544.356226/94‑1 (MQ=60)
                                                          TCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCA                                    <  SRR4302544.2080555/94‑1 (MQ=60)
                                                               TCCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGAT                               >  SRR4302544.4467052/1‑94 (MQ=60)
                                                               CCCAGCCAGAACTCGGTTTGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGAT                               <  SRR4302544.2322332/94‑1 (MQ=60)
                                                                CCAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATA                              >  SRR4302544.1683360/1‑94 (MQ=60)
                                                                 CAGCCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATAT                             <  SRR4302544.3381226/94‑1 (MQ=60)
                                                                    CCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCA                          >  SRR4302544.3127796/1‑94 (MQ=60)
                                                                    CCAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCA                          <  SRR4302544.1448955/94‑1 (MQ=60)
                                                                     CAGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCAT                         >  SRR4302544.3787587/1‑94 (MQ=60)
                                                                      AGAACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATC                        >  SRR4302544.2576849/1‑94 (MQ=60)
                                                                        AACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAA                      <  SRR4302544.2427722/94‑1 (MQ=60)
                                                                         ACTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAAC                     <  SRR4302544.3133507/94‑1 (MQ=60)
                                                                          CTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACG                    >  SRR4302544.2132192/1‑94 (MQ=60)
                                                                          CTCGGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACG                    <  SRR4302544.1547514/94‑1 (MQ=60)
                                                                             GGTTCGAGTCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACA                 >  SRR4302544.83858/1‑94 (MQ=60)
                                                                                     TCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGG         <  SRR4302544.4203472/94‑1 (MQ=60)
                                                                                     TCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGG         <  SRR4302544.4092418/94‑1 (MQ=60)
                                                                                     TCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGG         <  SRR4302544.4092416/94‑1 (MQ=60)
                                                                                     TCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGG         <  SRR4302544.3706723/94‑1 (MQ=60)
                                                                                     TCGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGG         <  SRR4302544.2436629/94‑1 (MQ=60)
                                                                                      CGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGC        >  SRR4302544.4760347/1‑94 (MQ=60)
                                                                                      CGCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGA        >  SRR4302544.2163452/1‑94 (MQ=60)
                                                                                       GCAGGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGAC       <  SRR4302544.1635760/94‑1 (MQ=60)
                                                                                          GGCGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTC    >  SRR4302544.671427/1‑94 (MQ=60)
                                                                                            CGCTTTAAACAGATATCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCAGCAACGACAAAATCCGGACGTCCG  >  SRR4302544.2975143/1‑94 (MQ=60)
                                                                                            CGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTCCG  >  SRR4302544.191735/1‑94 (MQ=60)
                                                                                            CGCTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTCCG  >  SRR4302544.1756085/1‑94 (MQ=60)
                                                                                             |                                                                                            
CAGCCGAGTTCCTGCAAGCGACTGATATCGCGGCGATCGCGCTCAACATTTTTACCTATCTTCTCCAGCCAGAACTCGGTTCGAGTCGCAGGCACTTTAAACAGATAGCAGTGATGATGATGCCAGAAGCAGCCATGGGTAAATATCACGCAGCGATATTCATCAACGACAAAATCCGGACGTCCG  >  NC_000913/2028615‑2028800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑