Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,043,272 A→G S130G (AGT→GGT)  b1978 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,043,2720AG100.0% 518.1 / NA 158S130G (AGT→GGT) b1978hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (66/92);  total (66/92)

Only 100 of 162 total aligned reads displayed.

GCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT  >  NC_000913/2043182‑2043361
                                                                                          |                                                                                         
gCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGt                                                                                         >  1:2271484/1‑93 (MQ=255)
gCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGt                                                                                         >  1:2271483/1‑93 (MQ=255)
 cGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGtt                                                                                        <  1:2641937/93‑1 (MQ=255)
 cGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGtt                                                                                        <  1:2604475/93‑1 (MQ=255)
  gTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGTGGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGGGGttt                                                                                       >  1:1031996/1‑93 (MQ=255)
  gTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGttt                                                                                       >  2:474574/1‑93 (MQ=255)
  gTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGttt                                                                                       <  2:1392319/93‑1 (MQ=255)
  gTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGttt                                                                                       <  2:2734840/93‑1 (MQ=255)
   ttGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGtttt                                                                                      <  2:1540852/93‑1 (MQ=255)
       aaTCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATa                                                                                  >  2:2998133/1‑93 (MQ=255)
       aaTCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATa                                                                                  >  2:2998134/1‑93 (MQ=255)
        aTCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATaa                                                                                 >  1:3629664/1‑93 (MQ=255)
        aTCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATaa                                                                                 <  2:2866168/93‑1 (MQ=255)
        aTCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATaa                                                                                 <  1:1295340/93‑1 (MQ=255)
        aTCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATaa                                                                                 <  2:426311/93‑1 (MQ=255)
         tCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTTGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAt                                                                                >  1:517572/1‑93 (MQ=255)
         tCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAt                                                                                <  1:506208/93‑1 (MQ=255)
         tCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAt                                                                                >  1:2960424/1‑93 (MQ=255)
         tCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAt                                                                                <  2:4085732/93‑1 (MQ=255)
         tCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAt                                                                                <  1:1691238/93‑1 (MQ=255)
           ggCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGTCTGAGTTTCGCAAACTCAACCAGTTTCGTACGTTTGCGCGAGTTTTTGATAATGt                                                                              >  2:4212418/1‑93 (MQ=255)
           ggCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGt                                                                              <  1:172975/93‑1 (MQ=255)
            gCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTc                                                                             <  1:4107128/93‑1 (MQ=255)
            gCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTc                                                                             <  2:4201004/93‑1 (MQ=255)
             cccAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTcc                                                                            <  2:1274673/93‑1 (MQ=255)
             cccAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTcc                                                                            <  1:3582868/93‑1 (MQ=255)
                  aGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAg                                                                       <  1:4103662/93‑1 (MQ=255)
                  aGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAg                                                                       >  2:1480002/1‑93 (MQ=255)
                    cGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAggg                                                                     <  2:994967/93‑1 (MQ=255)
                     gTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGt                                                                    >  1:3939288/1‑93 (MQ=255)
                      ttGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtg                                                                   <  1:308991/93‑1 (MQ=255)
                      ttGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtg                                                                   <  1:4065593/93‑1 (MQ=255)
                      ttGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtg                                                                   >  2:542764/1‑93 (MQ=255)
                       tGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtga                                                                  <  1:959649/93‑1 (MQ=255)
                        gCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgat                                                                 <  1:3095282/93‑1 (MQ=255)
                         ccGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgatg                                                                >  2:3588755/1‑93 (MQ=255)
                         ccGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgatg                                                                >  2:2927615/1‑93 (MQ=255)
                         ccGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGATTCATACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgatg                                                                >  2:1673306/1‑93 (MQ=255)
                          cGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgatga                                                               >  1:3215794/1‑93 (MQ=255)
                          cGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgatga                                                               <  1:1983553/93‑1 (MQ=255)
                          cGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgatga                                                               >  2:2361053/1‑93 (MQ=255)
                           gAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGaa                                                              >  1:2441926/1‑93 (MQ=255)
                           gAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGaa                                                              >  2:3154343/1‑93 (MQ=255)
                           gAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGaa                                                              <  1:4648763/93‑1 (MQ=255)
                           gAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTCGATAATGTCCGCCAGGGTGATGaa                                                              >  1:2411429/1‑93 (MQ=255)
                            aaCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAc                                                             <  2:2554867/93‑1 (MQ=255)
                            aaCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAc                                                             <  1:786073/93‑1 (MQ=255)
                             aCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACt                                                            <  2:2511313/93‑1 (MQ=255)
                             aCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACt                                                            <  2:4660556/93‑1 (MQ=255)
                             aCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACt                                                            >  1:564709/1‑93 (MQ=255)
                             aCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACt                                                            <  2:2657648/93‑1 (MQ=255)
                              cGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTg                                                           <  2:2499005/93‑1 (MQ=255)
                                tttCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGa                                                         <  2:2154751/93‑1 (MQ=255)
                                    ggTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTc                                                     >  1:3515030/1‑93 (MQ=255)
                                      tATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTccc                                                   >  2:667755/1‑93 (MQ=255)
                                       aTTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTACCg                                                  >  1:273213/1‑93 (MQ=255)
                                         ttCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGc                                                <  2:3798327/93‑1 (MQ=255)
                                          tCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGca                                               >  2:1267552/1‑93 (MQ=255)
                                          tCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGca                                               <  1:2124755/93‑1 (MQ=255)
                                            ggTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGcaca                                             <  2:2342064/93‑1 (MQ=255)
                                             gTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACaa                                            >  2:3222360/1‑93 (MQ=255)
                                              tGGCTGAGTTACGCAAACTCAGCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAg                                           <  1:2832196/93‑1 (MQ=255)
                                                gCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGtt                                         <  1:3409252/93‑1 (MQ=255)
                                                  tGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAg                                       <  1:4226872/93‑1 (MQ=255)
                                                  tGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAg                                       >  1:1726549/1‑93 (MQ=255)
                                                    aGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCTCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTg                                     <  1:4410927/93‑1 (MQ=255)
                                                    aGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTg                                     <  1:4338402/93‑1 (MQ=255)
                                                     gTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGa                                    <  2:1928030/93‑1 (MQ=255)
                                                        aCGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCAGGCACAAGTTAGTGaaaa                                 >  2:1323923/1‑93 (MQ=255)
                                                         cGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaa                                <  1:1825304/93‑1 (MQ=255)
                                                            aaaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaaaa                             >  1:1391093/1‑93 (MQ=255)
                                                             aaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAt                            >  2:1122682/1‑93 (MQ=255)
                                                             aaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAt                            >  1:152325/1‑93 (MQ=255)
                                                             aaCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAt                            <  1:2147010/93‑1 (MQ=255)
                                                              aCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAtt                           >  2:3682195/1‑93 (MQ=255)
                                                                ccAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTaa                         <  2:4078336/92‑1 (MQ=255)
                                                                 cAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAc                        <  1:2522662/93‑1 (MQ=255)
                                                                  aaCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcc                       <  1:3975385/93‑1 (MQ=255)
                                                                  aaCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcc                       <  2:495821/93‑1 (MQ=255)
                                                                  aaCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcc                       >  1:129292/1‑93 (MQ=255)
                                                                   aCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCTGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAccc                      <  1:2948484/93‑1 (MQ=255)
                                                                    ccAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcccc                     >  1:964328/1‑93 (MQ=255)
                                                                     cAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcccag                    >  2:606839/1‑91 (MQ=255)
                                                                       gTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgcc                  >  1:3497407/1‑93 (MQ=255)
                                                                       gTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAATAAACccggc                  >  1:1185587/1‑91 (MQ=255)
                                                                          tCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCTCGGCACAAGTTAGTGAAAAAAAATTAACCccgccgcc               >  1:33278/1‑93 (MQ=255)
                                                                           cGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgccgccg              >  1:729323/1‑93 (MQ=255)
                                                                            gTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCgg             >  2:2892179/1‑93 (MQ=255)
                                                                             tACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCggg            >  2:4089534/1‑93 (MQ=255)
                                                                               cGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTa          >  2:3925255/1‑93 (MQ=255)
                                                                                gTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCCCCGGGTaa         >  2:1338785/1‑93 (MQ=255)
                                                                                    gCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGc     <  1:1781735/93‑1 (MQ=255)
                                                                                     cTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCa    <  2:391543/93‑1 (MQ=255)
                                                                                     cTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCa    <  2:1851029/93‑1 (MQ=255)
                                                                                     cTCGAGGTTTTGATAATGTCCGCCAGGGGCATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCa    <  1:4747746/93‑1 (MQ=255)
                                                                                      tCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAg   >  2:3896694/1‑93 (MQ=255)
                                                                                      tCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAg   <  1:2648284/93‑1 (MQ=255)
                                                                                      tCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAg   <  1:1563336/93‑1 (MQ=255)
                                                                                       cGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGt  <  2:4415322/93‑1 (MQ=255)
                                                                                       cGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGt  <  1:4495100/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT  >  NC_000913/2043182‑2043361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACA  >  NC_000913/2043179‑2043365
                                                                                             |                                                                                             
GGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAG                                                                                               <  SRR4302544.2253224/94‑1 (MQ=60)
GGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAG                                                                                               <  SRR4302544.1991059/94‑1 (MQ=60)
GGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAG                                                                                               <  SRR4302544.1991058/94‑1 (MQ=60)
 GAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGG                                                                                              <  SRR4302544.4780775/94‑1 (MQ=60)
 GAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGG                                                                                              <  SRR4302544.2887748/94‑1 (MQ=60)
  AGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGT                                                                                             >  SRR4302544.2292861/1‑94 (MQ=60)
  AGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGT                                                                                             >  SRR4302544.2292862/1‑94 (MQ=60)
  AGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGT                                                                                             <  SRR4302544.1589813/94‑1 (MQ=60)
    CGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGTGGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGGGGTTT                                                                                           >  SRR4302544.1042266/1‑94 (MQ=60)
    CGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTT                                                                                           <  SRR4302544.2629495/94‑1 (MQ=60)
    CGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTT                                                                                           <  SRR4302544.2667264/94‑1 (MQ=60)
      TTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTG                                                                                         <  SRR4302544.66459/94‑1 (MQ=60)
      TTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTG                                                                                         <  SRR4302544.547660/94‑1 (MQ=60)
       TGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGA                                                                                        <  SRR4302544.1323026/94‑1 (MQ=60)
         GAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATA                                                                                      >  SRR4302544.4523431/1‑94 (MQ=60)
         GAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATA                                                                                      >  SRR4302544.2028739/1‑94 (MQ=60)
          AATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAA                                                                                     >  SRR4302544.3665827/1‑94 (MQ=60)
           ATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTTGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAT                                                                                    >  SRR4302544.522685/1‑94 (MQ=60)
           ATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAT                                                                                    >  SRR4302544.2988842/1‑94 (MQ=60)
           ATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAT                                                                                    <  SRR4302544.1308096/94‑1 (MQ=60)
            TCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATG                                                                                   <  SRR4302544.1707927/94‑1 (MQ=60)
            TCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATG                                                                                   <  SRR4302544.511183/94‑1 (MQ=60)
              GGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTC                                                                                 <  SRR4302544.174682/94‑1 (MQ=60)
               GCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCC                                                                                <  SRR4302544.3397909/94‑1 (MQ=60)
               GCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCA                                                                                <  SRR4302544.4147967/94‑1 (MQ=60)
                CCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCG                                                                               <  SRR4302544.3617247/94‑1 (MQ=60)
                  CAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCC                                                                             <  SRR4302544.3751898/94‑1 (MQ=60)
                  CAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCC                                                                             <  SRR4302544.2339494/94‑1 (MQ=60)
                  CAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCC                                                                             >  SRR4302544.4700362/1‑94 (MQ=60)
                  CAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCC                                                                             >  SRR4302544.1482727/1‑94 (MQ=60)
                   AAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCA                                                                            >  SRR4302544.2652077/1‑94 (MQ=60)
                     AGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGG                                                                          >  SRR4302544.4339507/1‑94 (MQ=60)
                     AGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGG                                                                          <  SRR4302544.4144480/94‑1 (MQ=60)
                       CGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGT                                                                        >  SRR4302544.3978415/1‑94 (MQ=60)
                       CGTTGCCGAACGTTTCGGGATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGGTTGGTCGAGGTTTTGATAATGTCCGCCAGGGG                                                                        >  SRR4302544.3978416/1‑94 (MQ=60)
                         TTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGA                                                                      <  SRR4302544.4106073/94‑1 (MQ=60)
                         TTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGA                                                                      <  SRR4302544.312203/94‑1 (MQ=60)
                          TGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGAT                                                                     <  SRR4302544.969074/94‑1 (MQ=60)
                           GCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATG                                                                    <  SRR4302544.3124758/94‑1 (MQ=60)
                            CCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGA                                                                   >  SRR4302544.3246471/1‑94 (MQ=60)
                             CGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAA                                                                  <  SRR4302544.2002814/94‑1 (MQ=60)
                             CGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAA                                                                  >  SRR4302544.2465209/1‑94 (MQ=60)
                             CGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTCGATAATGTCCGCCAGGGTGATGAA                                                                  >  SRR4302544.2434351/1‑94 (MQ=60)
                              GAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAC                                                                 <  SRR4302544.4694780/94‑1 (MQ=60)
                               AACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACT                                                                >  SRR4302544.570232/1‑94 (MQ=60)
                               AACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACT                                                                <  SRR4302544.793676/94‑1 (MQ=60)
                                 CGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGG                                                              >  SRR4302544.1706043/1‑94 (MQ=60)
                                   TTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGAT                                                            <  SRR4302544.2695058/94‑1 (MQ=60)
                                      CGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTC                                                         >  SRR4302544.3548577/1‑94 (MQ=60)
                                         TATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCG                                                      <  SRR4302544.1771984/94‑1 (MQ=60)
                                         TATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTACCG                                                      >  SRR4302544.276086/1‑94 (MQ=60)
                                           TTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGC                                                    <  SRR4302544.2977018/94‑1 (MQ=60)
                                             TCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCAC                                                  <  SRR4302544.2145133/94‑1 (MQ=60)
                                                GTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAG                                               <  SRR4302544.500977/94‑1 (MQ=60)
                                                GTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAG                                               <  SRR4302544.4157544/94‑1 (MQ=60)
                                                 TGGCTGAGTTACGCAAACTCAGCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGT                                              <  SRR4302544.2859564/94‑1 (MQ=60)
                                                   GCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTA                                            <  SRR4302544.3441845/94‑1 (MQ=60)
                                                    CTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAG                                           >  SRR4302544.1743484/1‑94 (MQ=60)
                                                     TGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGT                                          <  SRR4302544.4269905/94‑1 (MQ=60)
                                                     TGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGT                                          <  SRR4302544.3121318/94‑1 (MQ=60)
                                                      GAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTG                                         <  SRR4302544.4077396/94‑1 (MQ=60)
                                                       AGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCTCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGA                                        <  SRR4302544.4455465/94‑1 (MQ=60)
                                                       AGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGA                                        >  SRR4302544.1743880/1‑94 (MQ=60)
                                                       AGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGA                                        <  SRR4302544.4382553/94‑1 (MQ=60)
                                                            CGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAA                                   <  SRR4302544.1843082/94‑1 (MQ=60)
                                                             CCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAA                                  <  SRR4302544.2268903/94‑1 (MQ=60)
                                                              CAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAA                                 >  SRR4302544.1404979/1‑94 (MQ=60)
                                                               AAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAT                                >  SRR4302544.153802/1‑94 (MQ=60)
                                                                AACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATT                               <  SRR4302544.2167464/94‑1 (MQ=60)
                                                                   TCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAC                            >  SRR4302544.245112/1‑94 (MQ=60)
                                                                    CAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACC                           <  SRR4302544.2595061/94‑1 (MQ=60)
                                                                    CAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACC                           <  SRR4302544.2546901/94‑1 (MQ=60)
                                                                    CAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACC                           >  SRR4302544.130481/1‑94 (MQ=60)
                                                                    CAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGCGATGAACTGGATGTCCCGGCACAGGTTAGTGAAAAAAAATTAAAC                           >  SRR4302544.3602365/1‑94 (MQ=60)
                                                                     AACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCC                          <  SRR4302544.4014943/94‑1 (MQ=60)
                                                                     AACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCC                          <  SRR4302544.3537727/94‑1 (MQ=60)
                                                                     AACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCC                          >  SRR4302544.3284695/1‑94 (MQ=60)
                                                                      ACCAGTTTCGTTCGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCC                         <  SRR4302544.1220209/94‑1 (MQ=60)
                                                                      ACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCTGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCC                         <  SRR4302544.2976804/94‑1 (MQ=60)
                                                                      ACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCC                         >  SRR4302544.973795/1‑94 (MQ=60)
                                                                       CCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCG                        >  SRR4302544.4132578/1‑94 (MQ=60)
                                                                         AGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCC                      >  SRR4302544.3530759/1‑94 (MQ=60)
                                                                         AGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCC                      >  SRR4302544.816108/1‑94 (MQ=60)
                                                                         AGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAATAAACCCGGC                      >  SRR4302544.1197372/1‑94 (MQ=60)
                                                                            TTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCTCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCC                   >  SRR4302544.33599/1‑94 (MQ=60)
                                                                             TCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCG                  >  SRR4302544.736468/1‑94 (MQ=60)
                                                                               GTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGG                >  SRR4302544.1864580/1‑94 (MQ=60)
                                                                                       GCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCA        <  SRR4302544.1799161/94‑1 (MQ=60)
                                                                                        CTCGAGGTTTTGATAATGTCCGCCAGGGGCATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAG       <  SRR4302544.4794514/94‑1 (MQ=60)
                                                                                         TCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT      <  SRR4302544.2673681/94‑1 (MQ=60)
                                                                                         TCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT      <  SRR4302544.1578848/94‑1 (MQ=60)
                                                                                          CGAGGTTTTGATAATGTCCGGCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTG     <  SRR4302544.2907382/94‑1 (MQ=60)
                                                                                          CGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTG     <  SRR4302544.4540217/94‑1 (MQ=60)
                                                                                             GGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACA  >  SRR4302544.842923/1‑94 (MQ=60)
                                                                                             |                                                                                             
GGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACA  >  NC_000913/2043179‑2043365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑