Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,044,033	(C)_5→6	coding (1149/7152 nt)	b1978 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,044,028	1	.	C	95.9%	455.4 / 11.7	145	coding (1144/7152 nt)	b1978	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (5/1); new base C (92/47); total (97/48)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 167 total aligned reads displayed.

TCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑A‑‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACC  >  NC_000913/2043936‑2044114

tCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACt‑at‑a                                                                                           >  1:3962764/1‑93 (MQ=255)

 cTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑A‑‑c                                                                                        >  2:3741498/1‑93 (MQ=255)

  tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑A‑‑cc                                                                                       <  1:3391778/93‑1 (MQ=255)

  tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑A‑‑cc                                                                                       >  1:4375111/1‑93 (MQ=255)

  tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑A‑‑cc                                                                                       >  1:67831/1‑93 (MQ=255)

    tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑A‑‑cc‑cc                                                                                    >  2:1585009/1‑93 (MQ=255)

     gAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑A‑‑cc‑ccc                                                                                   >  2:1410132/1‑93 (MQ=255)

       aCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑A‑‑cc‑cccct                                                                                 >  2:1207698/1‑91 (MQ=255)

       aCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑A‑‑cc‑cccct                                                                                 <  1:2237856/93‑3 (MQ=255)

         aGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑A‑‑cc‑ccccttc                                                                               >  2:4663947/1‑89 (MQ=255)

         aGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑A‑‑cc‑ccccttc                                                                               <  2:144424/93‑5 (MQ=255)

           tattatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTccc                                                                              <  1:2261775/93‑1 (MQ=255)

            attatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCg                                                                             >  1:1959791/1‑93 (MQ=255)

            attatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCg                                                                             <  2:3251477/93‑1 (MQ=255)

               atGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCtg                                                                          >  1:2737755/1‑93 (MQ=255)

                   cGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCtgatga                                                                      <  1:70088/93‑1 (MQ=255)

                    gATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGAc                                                                     >  2:3650849/1‑93 (MQ=255)

                     aTGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGAcc                                                                    >  1:3513046/1‑93 (MQ=255)

                     aTGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGAcc                                                                    >  2:4269400/1‑93 (MQ=255)

                      tGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCt                                                                   >  2:1810320/1‑93 (MQ=255)

                      tGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCt                                                                   <  2:3604316/93‑1 (MQ=255)

                      tGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCt                                                                   <  2:1354033/93‑1 (MQ=255)

                       gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCtt                                                                  >  1:3696016/1‑93 (MQ=255)

                       gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCtt                                                                  >  2:265481/1‑93 (MQ=255)

                        aaGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTc                                                                 <  1:2643615/93‑1 (MQ=255)

                        aaGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTc                                                                 <  1:112732/93‑1 (MQ=255)

                         aGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCa                                                                <  2:3036865/93‑1 (MQ=255)

                          gTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAg                                                               >  2:2634408/1‑93 (MQ=255)

                           tGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAgc                                                              <  1:865284/93‑1 (MQ=255)

                             gCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAgcgc                                                            <  1:1200916/93‑1 (MQ=255)

                             gCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAgcgc                                                            >  1:1384410/1‑93 (MQ=255)

                              cccTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGcgcg                                                           >  2:1611477/1‑93 (MQ=255)

                              cccTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGcgcg                                                           >  2:4517229/1‑93 (MQ=255)

                               ccTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCgg                                                          >  2:4302608/1‑93 (MQ=255)

                               ccTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCgg                                                          >  2:458765/1‑93 (MQ=255)

                                ctcctCGATAAAGACGATCGTCAAATTAATCCTCATGCCATCACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTCCCCGCTGATGACCTTCAGCGCGGa                                                         <  1:4143418/89‑1 (MQ=255)

                                cTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑ATCC‑‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGa                                                         <  1:4143417/93‑1 (MQ=255)

                                cTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGa                                                         >  1:1511415/1‑93 (MQ=255)

                                 tGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAg                                                        <  1:4480921/93‑1 (MQ=255)

                                  gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGAGCTTCACCGCGGAGc                                                       >  2:81585/1‑93 (MQ=255)

                                  gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAga                                                       >  1:2024034/1‑92 (MQ=255)

                                  gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGc                                                       >  1:570276/1‑93 (MQ=255)

                                     cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAAc                                                    <  2:3931771/93‑1 (MQ=255)

                                     cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAAc                                                    <  1:3056058/93‑1 (MQ=255)

                                      taTAAAGATGCTCGGCAAAGAAATCCTCGTGCCATATCCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACg                                                   <  2:1625471/92‑1 (MQ=255)

                                      gATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACg                                                   >  2:1943540/1‑93 (MQ=255)

                                       aTAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGc                                                  >  1:4063234/1‑93 (MQ=255)

                                       aTAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGc                                                  >  1:311086/1‑93 (MQ=255)

                                        tAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGcc                                                 <  1:4614848/93‑1 (MQ=255)

                                        tAAAGACGATCGGCAAAGGAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGcc                                                 >  1:3560338/1‑93 (MQ=255)

                                        aaaaGACCAACGGCAAAGTAATCCTCAAGCCAAAACCGCTTGACTTAACTAAT‑C‑‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGcc                                                 <  2:2121920/92‑1 (MQ=255)

                                          aggACGATCGGCAAAGTAATCCGCAGGCAAAAGCCGATGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAg                                               <  1:1013736/91‑1 (MQ=255)

                                          aaGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAg                                               <  1:536855/93‑1 (MQ=255)

                                          aaGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAg                                               <  1:4199855/93‑1 (MQ=255)

                                          aaGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCAAg                                               >  2:1632336/1‑93 (MQ=255)

                                           aGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAgg                                              >  2:2354883/1‑93 (MQ=255)

                                            gACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAggg                                             >  1:1102943/1‑93 (MQ=255)

                                             aCGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGt                                            <  2:509873/93‑1 (MQ=255)

                                              cGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTa                                           <  1:3027107/93‑1 (MQ=255)

                                                aTCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTaaa                                         <  2:4376879/93‑1 (MQ=255)

                                                aTCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTaaa                                         >  1:2459115/1‑93 (MQ=255)

                                                 tCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAAc                                        >  2:2311508/1‑93 (MQ=255)

                                                  cGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACa                                       <  1:1287745/93‑1 (MQ=255)

                                                   ggCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAg                                      <  2:634575/93‑1 (MQ=255)

                                                    gCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAgg                                     <  1:3031597/93‑1 (MQ=255)

                                                      aaaGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGc                                   <  2:529414/93‑1 (MQ=255)

                                                       aaGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCg                                  >  2:1479762/1‑93 (MQ=255)

                                                       aaGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCg                                  >  1:500484/1‑93 (MQ=255)

                                                         gTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaa                                >  2:954145/1‑93 (MQ=255)

                                                          tAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaa                               >  2:2161206/1‑93 (MQ=255)

                                                           aaTCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaaa                              >  2:678443/1‑93 (MQ=255)

                                                           aaTCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaaa                              >  1:3512420/1‑93 (MQ=255)

                                                           aaTCATCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGaaaa                              <  2:2921177/93‑1 (MQ=255)

                                                            aTCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAAt                             >  1:2994322/1‑93 (MQ=255)

                                                             tCCTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGAGCGAAAATg                            >  1:2907172/1‑93 (MQ=255)

                                                              ccTCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGa                           <  2:362801/93‑1 (MQ=255)

                                                                tCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGaca                         >  1:1874221/1‑93 (MQ=255)

                                                                tCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGaca                         >  2:2711260/1‑93 (MQ=255)

                                                                tCATGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGaca                         >  2:4240447/1‑93 (MQ=255)

                                                                  aTGCCATAACCGCTGGACTTAACT‑AT‑A‑‑CCACCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACAcc                       >  1:3016330/1‑93 (MQ=255)

                                                                   tGCCATAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACAccc                      >  2:238210/1‑93 (MQ=255)

                                                                      cATAACCGCTGGACTTAACT‑AT‑ACCCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGt                    <  2:3159898/93‑1 (MQ=255)

                                                                       aTAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGttt                  >  2:254094/1‑93 (MQ=255)

                                                                       aTAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGttt                  >  2:2526407/1‑93 (MQ=255)

                                                                        tAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtttt                 >  2:3766724/1‑93 (MQ=255)

                                                                        tAACCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGtttt                 >  1:780834/1‑93 (MQ=255)

                                                                         aaCCGCTGGACTTAACT‑AT‑A‑‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGc               >  1:4529681/1‑93 (MQ=255)

                                                                          aCCGCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGc               <  2:2653789/93‑1 (MQ=255)

                                                                             gCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGt            >  2:3353622/1‑93 (MQ=255)

                                                                             gCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGt            <  1:4278365/93‑1 (MQ=255)

                                                                             gCTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGt            >  1:1502826/1‑93 (MQ=255)

                                                                              cTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTc           >  2:3444609/1‑93 (MQ=255)

                                                                              cTGGACTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTc           >  2:1852778/1‑93 (MQ=255)

                                                                                  aCTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAtt       <  2:123070/93‑1 (MQ=255)

                                                                                  aCTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAtt       >  1:941929/1‑93 (MQ=255)

                                                                                  aCTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAtt       >  1:941928/1‑93 (MQ=255)

                                                                                   cTTAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAttt      <  1:2511102/93‑1 (MQ=255)

                                                                                    ttAACT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAtttt     >  2:2002266/1‑93 (MQ=255)

                                                                                     tAACT‑AT‑A‑‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTac   >  1:2374108/1‑93 (MQ=255)

                                                                                      aaCT‑AT‑AC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTac   >  1:1935147/1‑93 (MQ=255)

TCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACT‑AT‑A‑‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACC  >  NC_000913/2043936‑2044114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGGC  >  NC_000913/2043943‑2044126

ACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑CC‑CCCctt                                                                                            <  SRR4302544.2258912/94‑4 (MQ=60)

   GTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCC                                                                                          >  SRR4302544.273910/1‑94 (MQ=60)

   GTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCC                                                                                          >  SRR4302544.2645711/1‑94 (MQ=60)

    TATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCG                                                                                         <  SRR4302544.2283053/94‑1 (MQ=60)

    TATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCG                                                                                         >  SRR4302544.1978808/1‑94 (MQ=60)

     ATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGC                                                                                        >  SRR4302544.3071906/1‑94 (MQ=60)

      TTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCT                                                                                       <  SRR4302544.4700747/94‑1 (MQ=60)

       TATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTG                                                                                      >  SRR4302544.2764141/1‑94 (MQ=60)

            CGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGAC                                                                                 <  SRR4302544.70773/94‑1 (MQ=60)

             GATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTACCTAT‑ACCC‑CCCTTCCCGCTGATGACC                                                                                <  SRR4302544.4223087/94‑1 (MQ=60)

             GATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACC                                                                                >  SRR4302544.3546562/1‑94 (MQ=60)

               TGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTT                                                                              >  SRR4302544.3732827/1‑94 (MQ=60)

               TGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTT                                                                              >  SRR4302544.2403135/1‑94 (MQ=60)

                 AAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCA                                                                            <  SRR4302544.113762/94‑1 (MQ=60)

                 AAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCA                                                                            <  SRR4302544.2668961/94‑1 (MQ=60)

                    TGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCG                                                                         <  SRR4302544.873844/94‑1 (MQ=60)

                    TGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCACTGGACTTAACTAT‑A‑CC‑CCCTTCCCGCTGATGACCTTCAGCGC                                                                        >  SRR4302544.4079306/1‑94 (MQ=60)

                     GGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGC                                                                        >  SRR4302544.1398237/1‑94 (MQ=60)

                      GCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCG                                                                       <  SRR4302544.1212813/94‑1 (MQ=60)

                       CCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGG                                                                      >  SRR4302544.4161426/1‑94 (MQ=60)

                       CCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGG                                                                      >  SRR4302544.1125816/1‑94 (MQ=60)

                        CCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGA                                                                     >  SRR4302544.1526393/1‑94 (MQ=60)

                         ctccTCGATAAAGACGATCGTCAAATTAATCCTCATGCCATCACCGCTGGACTTAACTAT‑ACCC‑CCCTCCCCGCTGATGACCTTCAGCGCGGAG                                                                    <  SRR4302544.4184436/90‑1 (MQ=60)

                         CTGTTCGATAAAGCCGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAG                                                                    <  SRR4302544.1144606/94‑1 (MQ=60)

                         CTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATCC‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAG                                                                    <  SRR4302544.4184435/94‑1 (MQ=60)

                          TGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGC                                                                   <  SRR4302544.4525962/94‑1 (MQ=60)

                          TGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGC                                                                   >  SRR4302544.3732158/1‑94 (MQ=60)

                          TGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGC                                                                   >  SRR4302544.575850/1‑94 (MQ=60)

                          TGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGA                                                                   >  SRR4302544.2043665/1‑94 (MQ=60)

                              CGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACG                                                               <  SRR4302544.3085234/94‑1 (MQ=60)

                               GATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGC                                                              >  SRR4302544.314317/1‑94 (MQ=60)

                               GATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGC                                                              >  SRR4302544.4103696/1‑94 (MQ=60)

                                ATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCC                                                             <  SRR4302544.2366592/94‑1 (MQ=60)

                                ATAAAGACGATCGGCAAAGGAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCC                                                             >  SRR4302544.3594471/1‑94 (MQ=60)

                                 TAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCA                                                            >  SRR4302544.841890/1‑94 (MQ=60)

                                 TAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCA                                                            <  SRR4302544.4660660/94‑1 (MQ=60)

                                   AGGACGATCGGCAAAGTAATCCGCAGGCAAAAGCCGATGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGG                                                          <  SRR4302544.1023836/94‑1 (MQ=60)

                                   AAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGG                                                          <  SRR4302544.542162/94‑1 (MQ=60)

                                   AAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGG                                                          <  SRR4302544.542163/94‑1 (MQ=60)

                                   AAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGG                                                          <  SRR4302544.4241766/94‑1 (MQ=60)

                                    AGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGG                                                         >  SRR4302544.1113930/1‑94 (MQ=60)

                                    AGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGG                                                         >  SRR4302544.3593389/1‑94 (MQ=60)

                                       CGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAA                                                      <  SRR4302544.3056109/94‑1 (MQ=60)

                                        GATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAA                                                     >  SRR4302544.2482582/1‑94 (MQ=60)

                                        CATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAA                                                     <  SRR4302544.692059/94‑1 (MQ=60)

                                           CGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAG                                                  <  SRR4302544.1300427/94‑1 (MQ=60)

                                            GGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGG                                                 >  SRR4302544.4420544/1‑94 (MQ=60)

                                             GCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGG                                                <  SRR4302544.4531437/94‑1 (MQ=60)

                                             GCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGG                                                <  SRR4302544.3060642/94‑1 (MQ=60)

                                              CAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGC                                               <  SRR4302544.585527/94‑1 (MQ=60)

                                               AAAGTAATCCTCATGCCATAACCGGTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCG                                              >  SRR4302544.3172248/1‑94 (MQ=60)

                                               AAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCG                                              <  SRR4302544.2169477/94‑1 (MQ=60)

                                               AAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCG                                              >  SRR4302544.505401/1‑94 (MQ=60)

                                                AAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGA                                             >  SRR4302544.4252262/1‑94 (MQ=60)

                                                   TAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAA                                          >  SRR4302544.3545926/1‑94 (MQ=60)

                                                   TAATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAA                                          >  SRR4302544.3545927/1‑94 (MQ=60)

                                                    AATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAAT                                         <  SRR4302544.1892735/94‑1 (MQ=60)

                                                    AATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAAT                                         >  SRR4302544.2007538/1‑94 (MQ=60)

                                                    AATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAAT                                         >  SRR4302544.3023031/1‑94 (MQ=60)

                                                     ATCCTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGAGCGAAAATG                                        >  SRR4302544.2935152/1‑94 (MQ=60)

                                                        CTCATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACA                                     >  SRR4302544.1892338/1‑94 (MQ=60)

                                                          CATGCCATAACCGCTGGACTTAACTAT‑A‑CCACCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACC                                   >  SRR4302544.3045248/1‑94 (MQ=60)

                                                           ATGCCATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCC                                  >  SRR4302544.164718/1‑94 (MQ=60)

                                                               CATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTT                              >  SRR4302544.2969682/1‑94 (MQ=60)

                                                               CATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTT                              >  SRR4302544.3024556/1‑94 (MQ=60)

                                                                ATAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTT                             >  SRR4302544.788406/1‑94 (MQ=60)

                                                                 TAACCGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTG                            >  SRR4302544.901722/1‑94 (MQ=60)

                                                                 TAACCGCTGGACTTAACTAT‑A‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGC                           >  SRR4302544.4574999/1‑94 (MQ=60)

                                                                     CGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGT                        >  SRR4302544.1334707/1‑94 (MQ=60)

                                                                     CGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGT                        >  SRR4302544.1517700/1‑94 (MQ=60)

                                                                     CGCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGT                        >  SRR4302544.1517699/1‑94 (MQ=60)

                                                                      GCTGGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTC                       <  SRR4302544.4322054/94‑1 (MQ=60)

                                                                         GGACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGAT                    <  SRR4302544.3056915/94‑1 (MQ=60)

                                                                          GACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATT                   >  SRR4302544.951205/1‑94 (MQ=60)

                                                                          GACTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATT                   >  SRR4302544.951206/1‑94 (MQ=60)

                                                                            CTTAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTT                 <  SRR4302544.2535201/94‑1 (MQ=60)

                                                                             TTAACTAT‑A‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTAC               >  SRR4302544.2396580/1‑94 (MQ=60)

                                                                              TAACTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTAC               >  SRR4302544.1953877/1‑94 (MQ=60)

                                                                                 CTAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTG            >  SRR4302544.956242/1‑94 (MQ=60)

                                                                                  TAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGG           <  SRR4302544.2730034/94‑1 (MQ=60)

                                                                                  TAT‑ACCC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGG           <  SRR4302544.3520464/94‑1 (MQ=60)

                                                                                           CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTG    >  SRR4302544.1892120/1‑94 (MQ=60)

                                                                                            CCGTCCCGCTCATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGG   <  SRR4302544.1223734/94‑1 (MQ=60)

                                                                                             CTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGGC  <  SRR4302544.1661563/94‑1 (MQ=60)

                                                                                             CTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGGC  >  SRR4302544.4376397/1‑94 (MQ=60)

ACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTAT‑A‑CC‑CCCTTCCCGCTGATGACCTTCAGCGCGGAGCAACGCCAGGGTAAACAGGGCGAAAATGACACCCGTTTTGCCGTCGATTTTACCTGGCAACCTGGC  >  NC_000913/2043943‑2044126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`