Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,043,272	A→G	S130G (AGT→GGT)	b1978 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,043,272	0	A	G	100.0%	708.2 / NA	217	S130G (AGT→GGT)	b1978	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (99/118); total (99/118)

Only 100 of 221 total aligned reads displayed.

GCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT  >  NC_000913/2043182‑2043361

 cGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGtt                                                                                        <  2:2827741/93‑1 (MQ=255)

 cGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGtt                                                                                        <  2:1077493/93‑1 (MQ=255)

  gTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCACACTCCACCAGTTTCGTACGTTTGCTCGAGGttt                                                                                       >  1:6247552/1‑93 (MQ=255)

    tGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTg                                                                                     <  2:3971230/93‑1 (MQ=255)

    tGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTg                                                                                     <  2:4745613/93‑1 (MQ=255)

     ggAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGa                                                                                    <  2:4877352/93‑1 (MQ=255)

     ggAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGa                                                                                    >  2:5772735/1‑93 (MQ=255)

      gAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGAt                                                                                   <  2:3131022/93‑1 (MQ=255)

       aaTCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATa                                                                                  >  1:2054331/1‑93 (MQ=255)

        aTCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATaa                                                                                 >  1:824575/1‑93 (MQ=255)

        aTCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATaa                                                                                 >  1:3512836/1‑93 (MQ=255)

         tCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAt                                                                                >  1:3757079/1‑93 (MQ=255)

             cccAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTcc                                                                            <  1:3143086/93‑1 (MQ=255)

              ccAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCg                                                                           >  1:330971/1‑93 (MQ=255)

              ccAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCg                                                                           >  2:1930367/1‑93 (MQ=255)

              ccAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCg                                                                           >  1:1905970/1‑93 (MQ=255)

              ccAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCg                                                                           >  1:1578136/1‑93 (MQ=255)

               cAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGc                                                                          <  2:5690089/93‑1 (MQ=255)

               cAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGc                                                                          <  1:2851391/93‑1 (MQ=255)

                aaaGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGcc                                                                         <  2:6008563/93‑1 (MQ=255)

                aaaGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGcc                                                                         >  2:2412261/1‑93 (MQ=255)

                 aaGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCa                                                                        >  2:2502020/1‑93 (MQ=255)

                 aaGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCAGCCa                                                                        >  2:2400882/1‑93 (MQ=255)

                  aGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAg                                                                       <  1:6345221/93‑1 (MQ=255)

                   gCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAgg                                                                      >  2:2023584/1‑93 (MQ=255)

                     gTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGt                                                                    <  1:1323222/93‑1 (MQ=255)

                     gTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGt                                                                    <  1:4858538/93‑1 (MQ=255)

                     gTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGt                                                                    <  2:2963499/93‑1 (MQ=255)

                        gCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgat                                                                 <  2:1769859/93‑1 (MQ=255)

                        gCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgat                                                                 >  1:3351564/1‑93 (MQ=255)

                         ccGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgatg                                                                >  2:5299458/1‑93 (MQ=255)

                          cGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGtgatga                                                               >  2:4947361/1‑93 (MQ=255)

                           gAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGaa                                                              >  1:4740471/1‑93 (MQ=255)

                            aaCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAc                                                             >  2:1668800/1‑93 (MQ=255)

                            aaCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAc                                                             <  1:5784315/93‑1 (MQ=255)

                            aaCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAc                                                             <  2:4224307/93‑1 (MQ=255)

                            aaCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAc                                                             >  2:341174/1‑93 (MQ=255)

                            aaCGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAc                                                             <  2:4360170/93‑1 (MQ=255)

                              cGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTg                                                           <  1:2105118/93‑1 (MQ=255)

                               gTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTgg                                                          >  2:3688999/1‑93 (MQ=255)

                                tttCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGa                                                         >  1:6703042/1‑93 (MQ=255)

                                  tCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATg                                                       >  1:3706475/1‑93 (MQ=255)

                                   cGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGt                                                      <  1:4728100/93‑1 (MQ=255)

                                       aTTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCg                                                  <  1:5220767/93‑1 (MQ=255)

                                         ttCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGc                                                <  2:1864710/93‑1 (MQ=255)

                                           cGGTGGCTGAGTTACGCAAACTCAACCAGTGTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGcac                                              <  1:1726136/93‑1 (MQ=255)

                                            ggTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGcaca                                             >  1:1064473/1‑93 (MQ=255)

                                             gTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACaa                                            <  2:5431769/93‑1 (MQ=255)

                                             gTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACaa                                            >  2:5817924/1‑93 (MQ=255)

                                              tGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAg                                           <  1:1184180/93‑1 (MQ=255)

                                               ggCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGt                                          <  1:6048726/93‑1 (MQ=255)

                                                 cTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTa                                        <  2:104591/93‑1 (MQ=255)

                                                 cTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTa                                        <  2:6684991/93‑1 (MQ=255)

                                                 cTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTa                                        <  1:2398351/93‑1 (MQ=255)

                                                 cTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTa                                        <  1:2502517/93‑1 (MQ=255)

                                                 cTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTa                                        >  1:2933468/1‑93 (MQ=255)

                                                 cTGAGTGACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGGCCCGGCACAAGTTa                                        <  2:2846873/93‑1 (MQ=255)

                                                  tGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAg                                       >  1:3375030/1‑93 (MQ=255)

                                                  tGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAg                                       <  2:5138457/93‑1 (MQ=255)

                                                   gAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGt                                      >  1:4338392/1‑93 (MQ=255)

                                                   gAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGt                                      <  2:1443854/93‑1 (MQ=255)

                                                    aGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTg                                     >  2:4676655/1‑93 (MQ=255)

                                                     gTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGa                                    >  2:1618348/1‑93 (MQ=255)

                                                       tACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaa                                  >  1:5235426/1‑93 (MQ=255)

                                                          gCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACCAGTTAGTGaaaaaa                               >  1:4033946/1‑93 (MQ=255)

                                                          gCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaa                               <  2:5481461/93‑1 (MQ=255)

                                                           cAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaaa                              >  2:2307836/1‑93 (MQ=255)

                                                            aaaCTCAGCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGaaaaaaaa                             >  1:6606840/1‑93 (MQ=255)

                                                              aCTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAtt                           <  2:6390901/93‑1 (MQ=255)

                                                                tCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTaa                         <  2:4174728/93‑1 (MQ=255)

                                                                tCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTaa                         <  2:6573070/93‑1 (MQ=255)

                                                                tCAACCAGTTTCGTACGTTTGCTCGAGGCTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTaa                         >  1:6194507/1‑93 (MQ=255)

                                                                 cAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAc                        <  2:4999334/93‑1 (MQ=255)

                                                                  aaCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTaaac                       >  2:6059557/1‑91 (MQ=255)

                                                                  aaCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcc                       >  2:3507061/1‑93 (MQ=255)

                                                                   aCCAGTTTAGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGAGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAacc                      >  1:6568730/1‑93 (MQ=255)

                                                                    ccAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcccc                     >  1:6425693/1‑93 (MQ=255)

                                                                    ccAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAcccc                     <  1:4304084/93‑1 (MQ=255)

                                                                      aGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgc                   >  2:6184318/1‑93 (MQ=255)

                                                                      aGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgc                   <  2:4129003/93‑1 (MQ=255)

                                                                      aGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgc                   >  1:4980231/1‑93 (MQ=255)

                                                                        tttCGTACGTTTGCTCGAGGTTTTGATAATTGCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgccg                 <  1:699825/93‑1 (MQ=255)

                                                                           cGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCccgccgccg              <  1:2204722/93‑1 (MQ=255)

                                                                            gTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCgg             <  1:5484633/93‑1 (MQ=255)

                                                                             tACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCggg            >  1:1280065/1‑93 (MQ=255)

                                                                              aCGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGt           >  1:4424015/1‑93 (MQ=255)

                                                                               cGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCgggca          >  2:2287861/1‑91 (MQ=255)

                                                                               cGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTa          >  1:4546833/1‑93 (MQ=255)

                                                                                gTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTaa         >  2:1963914/1‑93 (MQ=255)

                                                                                gTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTaa         <  2:3195508/93‑1 (MQ=255)

                                                                                 tttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTCACCCCGCCTCCGGGTAAt        >  1:4921632/1‑93 (MQ=255)

                                                                                 tttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAAt        >  2:3758513/1‑93 (MQ=255)

                                                                                 tttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAAt        <  2:386821/93‑1 (MQ=255)

                                                                                  ttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATa       >  2:1481396/1‑93 (MQ=255)

                                                                                  ttGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATa       <  1:360188/93‑1 (MQ=255)

                                                                                     cTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCa    <  1:6594455/93‑1 (MQ=255)

                                                                                      tCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAg   >  1:2688825/1‑93 (MQ=255)

                                                                                      tCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAg   <  1:5576507/93‑1 (MQ=255)

                                                                                       cGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGt  >  1:3059606/1‑93 (MQ=255)

                                                                                       cGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGt  <  1:5641160/93‑1 (MQ=255)

GCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT  >  NC_000913/2043182‑2043361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACA  >  NC_000913/2043179‑2043365

GGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAG                                                                                               <  SRR4302536.2822119/94‑1 (MQ=60)

 GAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGG                                                                                              <  SRR4302536.3929737/94‑1 (MQ=60)

 GAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGG                                                                                              <  SRR4302536.4696946/94‑1 (MQ=60)

   GCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTT                                                                                            <  SRR4302536.6477267/94‑1 (MQ=60)

    CGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCACACTCCACCAGTTTCGTACGTTTGCTCGAGGTTT                                                                                           >  SRR4302536.6433657/1‑94 (MQ=60)

    CGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTT                                                                                           <  SRR4302536.1897123/94‑1 (MQ=60)

    CGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTT                                                                                           <  SRR4302536.3064928/94‑1 (MQ=60)

    CGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTT                                                                                           <  SRR4302536.6254592/94‑1 (MQ=60)

     GTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTT                                                                                          >  SRR4302536.5244714/1‑94 (MQ=60)

      TTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTG                                                                                         <  SRR4302536.6888074/94‑1 (MQ=60)

         GAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATA                                                                                      >  SRR4302536.2116200/1‑94 (MQ=60)

          AATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAA                                                                                     >  SRR4302536.848842/1‑94 (MQ=60)

          AATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAA                                                                                     >  SRR4302536.3617724/1‑94 (MQ=60)

           ATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAT                                                                                    >  SRR4302536.3869317/1‑94 (MQ=60)

           ATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAAT                                                                                    >  SRR4302536.746446/1‑94 (MQ=60)

           ATCGGCCCAAAGCGTTGCCGAACGTTTCGGTAATTCGGTGGCTGAGCTACGGAAACTCAACCAGTTTCGTAGGTTTGCTCGAGGTTTTTGTAAT                                                                                    >  SRR4302536.3976448/1‑94 (MQ=60)

            TCGTCCCAAAGCGTGGCCGAACGTTTCGTTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATG                                                                                   <  SRR4302536.1685647/94‑1 (MQ=60)

            TCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATG                                                                                   <  SRR4302536.4876929/94‑1 (MQ=60)

             CGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGT                                                                                  <  SRR4302536.4542765/94‑1 (MQ=60)

             CGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGT                                                                                  <  SRR4302536.5833965/94‑1 (MQ=60)

              GGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTC                                                                                 <  SRR4302536.21876/94‑1 (MQ=60)

               GCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCC                                                                                >  SRR4302536.440560/1‑94 (MQ=60)

                CCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCG                                                                               <  SRR4302536.3235116/94‑1 (MQ=60)

                CCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCG                                                                               >  SRR4302536.339757/1‑94 (MQ=60)

                CCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCG                                                                               >  SRR4302536.1963352/1‑94 (MQ=60)

                CCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCG                                                                               >  SRR4302536.1624377/1‑94 (MQ=60)

                 CCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGC                                                                              <  SRR4302536.4939038/94‑1 (MQ=60)

                 CCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGC                                                                              >  SRR4302536.1155800/1‑94 (MQ=60)

                 CCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACCCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGCTTTGATAATGTCCGC                                                                              >  SRR4302536.889740/1‑94 (MQ=60)

                  CAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCC                                                                             <  SRR4302536.2935684/94‑1 (MQ=60)

                   AAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCA                                                                            >  SRR4302536.4852828/1‑94 (MQ=60)

                     AGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGG                                                                          <  SRR4302536.6534250/94‑1 (MQ=60)

                        GTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTG                                                                       <  SRR4302536.5004755/94‑1 (MQ=60)

                        GTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTG                                                                       >  SRR4302536.122461/1‑94 (MQ=60)

                        GTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTG                                                                       <  SRR4302536.1362725/94‑1 (MQ=60)

                        GTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTG                                                                       <  SRR4302536.5768196/94‑1 (MQ=60)

                        GTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTG                                                                       <  SRR4302536.361394/94‑1 (MQ=60)

                        GTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTG                                                                       <  SRR4302536.3567548/94‑1 (MQ=60)

                          TGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGAT                                                                     >  SRR4302536.3450990/1‑94 (MQ=60)

                            CCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGA                                                                   <  SRR4302536.484850/94‑1 (MQ=60)

                            CCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGA                                                                   >  SRR4302536.3738017/1‑94 (MQ=60)

                             CGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAA                                                                  >  SRR4302536.4883113/1‑94 (MQ=60)

                              GAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAAC                                                                 >  SRR4302536.687923/1‑94 (MQ=60)

                               AACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACT                                                                <  SRR4302536.5956480/94‑1 (MQ=60)

                               AACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACT                                                                <  SRR4302536.3154330/94‑1 (MQ=60)

                                ACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTG                                                               >  SRR4302536.4039513/1‑94 (MQ=60)

                                 CGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGG                                                              <  SRR4302536.5941833/94‑1 (MQ=60)

                                 CGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGG                                                              <  SRR4302536.2168395/94‑1 (MQ=60)

                                  GTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGA                                                             >  SRR4302536.6902114/1‑94 (MQ=60)

                                  GTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGA                                                             >  SRR4302536.711438/1‑94 (MQ=60)

                                    TTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATG                                                           >  SRR4302536.3817288/1‑94 (MQ=60)

                                      CGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTC                                                         >  SRR4302536.2531841/1‑94 (MQ=60)

                                      CGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTC                                                         <  SRR4302536.6418723/94‑1 (MQ=60)

                                      CGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTC                                                         <  SRR4302536.2007773/94‑1 (MQ=60)

                                      CGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTC                                                         <  SRR4302536.4870365/94‑1 (MQ=60)

                                          ATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGG                                                     <  SRR4302536.5376325/94‑1 (MQ=60)

                                              CGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACA                                                 >  SRR4302536.3843723/1‑94 (MQ=60)

                                              CGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACA                                                 >  SRR4302536.1096308/1‑94 (MQ=60)

                                              CGGTGGCTGAGTTACGCAAACTCAACCAGTGTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACA                                                 <  SRR4302536.1775979/94‑1 (MQ=60)

                                                GTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAG                                               <  SRR4302536.1410167/94‑1 (MQ=60)

                                                GTGGCTGAGTTACGCAAACTCAACCAGTTGCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAG                                               <  SRR4302536.6794997/94‑1 (MQ=60)

                                                 TGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGT                                              >  SRR4302536.6689232/1‑94 (MQ=60)

                                                 TGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGT                                              <  SRR4302536.1219819/94‑1 (MQ=60)

                                                  GGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTT                                             <  SRR4302536.6229141/94‑1 (MQ=60)

                                                   GCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTA                                            >  SRR4302536.3020032/1‑94 (MQ=60)

                                                    CTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAG                                           <  SRR4302536.2577966/94‑1 (MQ=60)

                                                    CTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAG                                           >  SRR4302536.6790560/1‑94 (MQ=60)

                                                    CTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAG                                           >  SRR4302536.3475301/1‑94 (MQ=60)

                                                    CTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAG                                           <  SRR4302536.2470948/94‑1 (MQ=60)

                                                     TGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGT                                          <  SRR4302536.3372318/94‑1 (MQ=60)

                                                     TGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGT                                          >  SRR4302536.4468330/1‑94 (MQ=60)

                                                       AGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGA                                        >  SRR4302536.570164/1‑94 (MQ=60)

                                                         TTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAA                                      <  SRR4302536.3467324/94‑1 (MQ=60)

                                                         TTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAA                                      <  SRR4302536.1640532/94‑1 (MQ=60)

                                                         TTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAA                                      >  SRR4302536.5391412/1‑94 (MQ=60)

                                                            CGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACCAGTTAGTGAAAAAA                                   >  SRR4302536.4153582/1‑94 (MQ=60)

                                                            CGCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAA                                   <  SRR4302536.6591704/94‑1 (MQ=60)

                                                             GCAAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAA                                  >  SRR4302536.2331656/1‑94 (MQ=60)

                                                              CAAACTCAGCCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAA                                 >  SRR4302536.6803303/1‑94 (MQ=60)

                                                               AAACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAAA                                <  SRR4302536.2586278/94‑1 (MQ=60)

                                                                AACTCAACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATT                               >  SRR4302536.5083370/1‑94 (MQ=60)

                                                                  CTCAACCAGTTTCGTACGTTTGCTCGAGGCTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAA                             >  SRR4302536.6379017/1‑94 (MQ=60)

                                                                     AACCAGTTTAGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGAGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAAACC                          >  SRR4302536.6764226/1‑94 (MQ=60)

                                                                      ACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCC                         <  SRR4302536.1333046/94‑1 (MQ=60)

                                                                      ACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCC                         >  SRR4302536.6617749/1‑94 (MQ=60)

                                                                      ACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCC                         <  SRR4302536.2862617/94‑1 (MQ=60)

                                                                      ACCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCC                         <  SRR4302536.3446055/94‑1 (MQ=60)

                                                                       CCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCG                        <  SRR4302536.4432793/94‑1 (MQ=60)

                                                                       CCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCG                        <  SRR4302536.5041669/94‑1 (MQ=60)

                                                                       CCAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCG                        <  SRR4302536.2160671/94‑1 (MQ=60)

                                                                        CAGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGC                       >  SRR4302536.5129785/1‑94 (MQ=60)

                                                                         AGTTTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCC                      <  SRR4302536.6182101/94‑1 (MQ=60)

                                                                           TTTCGTACGTTTGCTCGAGGTTTTGATAATTGCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGC                    <  SRR4302536.720265/94‑1 (MQ=60)

                                                                            TTCGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCC                   <  SRR4302536.4574046/94‑1 (MQ=60)

                                                                              CGTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGG                 <  SRR4302536.2271290/94‑1 (MQ=60)

                                                                               GTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGG                <  SRR4302536.5648373/94‑1 (MQ=60)

                                                                               GTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGG                >  SRR4302536.5384869/1‑94 (MQ=60)

                                                                               GTACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGG                >  SRR4302536.1318378/1‑94 (MQ=60)

                                                                                TACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGT               >  SRR4302536.3575565/1‑94 (MQ=60)

                                                                                TACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGT               >  SRR4302536.4556526/1‑94 (MQ=60)

                                                                                 ACGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTA              >  SRR4302536.4683192/1‑94 (MQ=60)

                                                                                  CGTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAA             <  SRR4302536.2311670/94‑1 (MQ=60)

                                                                                   GTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTCACCCCGCCTCCGGGTAAT            >  SRR4302536.5069532/1‑94 (MQ=60)

                                                                                   GTTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAAT            >  SRR4302536.6467486/1‑94 (MQ=60)

                                                                                    TTTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATA           <  SRR4302536.3022384/94‑1 (MQ=60)

                                                                                     TTGCTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAG          <  SRR4302536.369709/94‑1 (MQ=60)

                                                                                        CTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAG       <  SRR4302536.6790619/94‑1 (MQ=60)

                                                                                        CTCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAG       >  SRR4302536.2769106/1‑94 (MQ=60)

                                                                                         TCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT      <  SRR4302536.5742490/94‑1 (MQ=60)

                                                                                         TCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT      <  SRR4302536.5250893/94‑1 (MQ=60)

                                                                                         TCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT      >  SRR4302536.3149396/1‑94 (MQ=60)

                                                                                         TCGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGT      <  SRR4302536.2947264/94‑1 (MQ=60)

                                                                                          CGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTG     <  SRR4302536.5808759/94‑1 (MQ=60)

                                                                                          CGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTG     <  SRR4302536.5640953/94‑1 (MQ=60)

                                                                                          CGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTG     <  SRR4302536.3940623/94‑1 (MQ=60)

                                                                                          CGAGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTG     <  SRR4302536.4450491/94‑1 (MQ=60)

                                                                                          CGAGGTTTTGATAATGTCCGCCAGGGGGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACACCGCCGCCGGGTAATAGCAGTG     >  SRR4302536.4672816/1‑94 (MQ=60)

                                                                                            AGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGAC   <  SRR4302536.5524818/94‑1 (MQ=60)

                                                                                            AGGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGAC   >  SRR4302536.862001/1‑94 (MQ=60)

                                                                                             GGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACA  >  SRR4302536.198033/1‑94 (MQ=60)

GGAGCGTTGGAATCGGCCCAAAGCGTTGCCGAACGTTTCGGTATTTCGGTGGCTGAGTTACGCAAACTCAACCAGTTTCGTACGTTTGCTCGAAGTTTTGATAATGTCCGCCAGGGTGATGAACTGGATGTCCCGGCACAAGTTAGTGAAAAAAAATTAACCCCGCCGCCGGGTAATAGCAGTGACA  >  NC_000913/2043179‑2043365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`