Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,078,049	A→G	F189L (TTC→CTC)	yeeA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,078,049	0	A	G	99.2%	392.9 / ‑5.2	121	F189L (TTC→CTC)	yeeA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (47/73); total (47/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 128 total aligned reads displayed.

GCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGG  >  NC_000913/2077959‑2078138

 cgcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGc                                                                                        <  2:2994844/93‑1 (MQ=255)

 cgcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGc                                                                                        <  2:2511242/93‑1 (MQ=255)

 cccAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGc                                                                                        <  1:952024/91‑1 (MQ=255)

    aaTCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGa                                                                                     >  1:2690337/1‑93 (MQ=255)

    aaTCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGa                                                                                     <  1:3435369/93‑1 (MQ=255)

    aaTCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGa                                                                                     <  1:3435368/93‑1 (MQ=255)

     aTCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAt                                                                                    >  2:3897524/1‑93 (MQ=255)

     aTCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCAAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAt                                                                                    <  1:3093449/93‑1 (MQ=255)

      tCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAtt                                                                                   >  2:2636551/1‑93 (MQ=255)

      tCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAtt                                                                                   <  1:2907867/93‑1 (MQ=255)

      tCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAtt                                                                                   <  2:3261347/93‑1 (MQ=255)

      tCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAtt                                                                                   <  1:3304871/93‑1 (MQ=255)

      tCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAtt                                                                                   <  1:4432256/93‑1 (MQ=255)

      tCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAtt                                                                                   >  1:596493/1‑93 (MQ=255)

      tCAGTCCACGCAGTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGAtt                                                                                   <  1:4035706/93‑1 (MQ=255)

      acAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAtt                                                                                   <  1:1109946/92‑1 (MQ=255)

       cAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTg                                                                                  <  1:1821057/93‑1 (MQ=255)

         gTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAt                                                                                >  1:47317/1‑93 (MQ=255)

         gTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAt                                                                                <  2:3037361/93‑1 (MQ=255)

         gTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAt                                                                                >  2:4093624/1‑93 (MQ=255)

          tCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATa                                                                               <  2:2101592/93‑1 (MQ=255)

          tCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATa                                                                               <  1:3264431/93‑1 (MQ=255)

             aCGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAc                                                                            <  1:4690601/93‑1 (MQ=255)

             aCGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAc                                                                            >  1:3001004/1‑93 (MQ=255)

              cGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAcc                                                                           <  2:4160895/93‑1 (MQ=255)

               gCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAccc                                                                          >  2:1647531/1‑93 (MQ=255)

                 aTTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTACGTTCGGTGAGAGTGCAGATTGAGAGCCCCgt                                                                        >  1:1381430/1‑92 (MQ=255)

                 aTTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGa                                                                        >  1:1381429/1‑93 (MQ=255)

                  ttttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGAt                                                                       <  2:2779290/93‑1 (MQ=255)

                  ttttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGAt                                                                       >  2:158222/1‑93 (MQ=255)

                  ttttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGAt                                                                       >  2:380584/1‑93 (MQ=255)

                   tttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGAtt                                                                      <  2:2936254/93‑1 (MQ=255)

                         ggCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTc                                                                <  1:91586/93‑1 (MQ=255)

                         ggCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTc                                                                <  2:721711/93‑1 (MQ=255)

                          gCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCg                                                               <  2:3730311/93‑1 (MQ=255)

                           cATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCgg                                                              <  2:1245382/93‑1 (MQ=255)

                            aTCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGt                                                             >  2:1128366/1‑93 (MQ=255)

                            aTCGGTCAGGAGTTTTTGCAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGt                                                             <  2:736090/93‑1 (MQ=255)

                             tCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTc                                                            >  2:118437/1‑93 (MQ=255)

                              cGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCa                                                           <  2:1178813/93‑1 (MQ=255)

                              cGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCa                                                           <  1:667653/93‑1 (MQ=255)

                                gTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGa                                                         >  1:2014218/1‑93 (MQ=255)

                                gTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGCAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGa                                                         >  2:1718517/1‑93 (MQ=255)

                                 tCATGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGAc                                                        <  1:2076388/93‑1 (MQ=255)

                                  cAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACt                                                       >  1:2684141/1‑93 (MQ=255)

                                   aGGAGTTTTTGTAGATGGCTTTCCAGGCGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACtt                                                      <  2:764851/93‑1 (MQ=255)

                                   aGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACtt                                                      >  2:4148881/1‑93 (MQ=255)

                                   aGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACtt                                                      >  2:3474173/1‑93 (MQ=255)

                                   aGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACtt                                                      <  2:177673/93‑1 (MQ=255)

                                   aGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACtt                                                      <  1:2672587/93‑1 (MQ=255)

                                   aGGAGTGTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTGCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACtt                                                      <  2:177672/93‑1 (MQ=255)

                                    ggAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACttt                                                     >  2:3096331/1‑93 (MQ=255)

                                      aGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTc                                                   <  2:3342673/93‑1 (MQ=255)

                                       gTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCg                                                  >  1:2633632/1‑93 (MQ=255)

                                         ttttGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGcc                                                <  2:1157534/93‑1 (MQ=255)

                                           ttGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAg                                              >  1:1057155/1‑93 (MQ=255)

                                           ttGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAg                                              <  2:511616/93‑1 (MQ=255)

                                             gTAGATTGCTGTCCATAGGTGGGCGTTTTAGTAAGGTCGGTGAGAGTGCAGATTGATAGACCCGATTATTCTCGGTCAGACTTTTCGCTAGtt                                            <  2:1031952/93‑1 (MQ=255)

                                              tAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGCGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTg                                           >  1:4665834/1‑93 (MQ=255)

                                               aGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGa                                          >  1:2197920/1‑93 (MQ=255)

                                                 aTGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAAt                                        <  1:1231840/93‑1 (MQ=255)

                                                  tGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATg                                       <  1:2618504/93‑1 (MQ=255)

                                                   ggCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATgc                                      <  1:3255312/93‑1 (MQ=255)

                                                      tttCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGcc                                   >  1:2169275/1‑93 (MQ=255)

                                                       ttCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCa                                  >  1:4420848/1‑93 (MQ=255)

                                                        tCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAg                                 <  2:1394320/93‑1 (MQ=255)

                                                         ccAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGt                                <  1:3499701/93‑1 (MQ=255)

                                                         ccAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGt                                <  2:1774240/93‑1 (MQ=255)

                                                          cAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTg                               <  2:2806912/93‑1 (MQ=255)

                                                           aGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTgg                              <  1:1514683/93‑1 (MQ=255)

                                                           aGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTgg                              >  2:3258620/1‑93 (MQ=255)

                                                            gACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCGGTTGCATGCGCCAGTggt                             >  1:1993722/1‑92 (MQ=255)

                                                            gACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGa                             >  1:3758430/1‑93 (MQ=255)

                                                            gACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGa                             >  2:1924483/1‑93 (MQ=255)

                                                            gACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGa                             >  1:3758431/1‑93 (MQ=255)

                                                             aCGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGAt                            >  2:4014542/1‑93 (MQ=255)

                                                             aCGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGAt                            <  1:4719384/93‑1 (MQ=255)

                                                              cGTGGGCGTTCGAGTAAGTTCGGTGAGAGGGCAGATTGATAGTCCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATg                           <  2:1808295/93‑1 (MQ=255)

                                                               gTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGa                          >  2:1229586/1‑93 (MQ=255)

                                                                 gggCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAAc                        <  2:2141004/93‑1 (MQ=255)

                                                                   gCGTTCGAGTAAGTTCGGTGAGAGTGCCGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGc                      <  1:1308687/93‑1 (MQ=255)

                                                                   gCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGc                      <  2:3747147/93‑1 (MQ=255)

                                                                    cGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGcc                     <  1:2662691/93‑1 (MQ=255)

                                                                     gTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGccc                    <  2:1689850/93‑1 (MQ=255)

                                                                      ttCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCg                   >  1:1248567/1‑93 (MQ=255)

                                                                      ttCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCg                   >  1:667652/1‑93 (MQ=255)

                                                                       tCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGt                  >  1:1935418/1‑93 (MQ=255)

                                                                        cGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGtt                 <  1:3179537/93‑1 (MQ=255)

                                                                        cGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGtt                 >  1:2967113/1‑93 (MQ=255)

                                                                         gAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtg                >  1:2341055/1‑93 (MQ=255)

                                                                         gAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtg                >  2:448195/1‑93 (MQ=255)

                                                                          aGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtgt               <  2:2433141/93‑1 (MQ=255)

                                                                           gTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtgtg              <  1:910726/93‑1 (MQ=255)

                                                                           gTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtgtg              <  1:1858199/93‑1 (MQ=255)

                                                                             aaGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGc            >  1:2504913/1‑93 (MQ=255)

                                                                                 tCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGa        >  1:1954812/1‑93 (MQ=255)

                                                                                  cGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGAt       <  2:2539579/93‑1 (MQ=255)

                                                                                   ggTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGAtc      >  1:4346600/1‑92 (MQ=255)

                                                                                     tGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATAcc    <  1:3361449/93‑1 (MQ=255)

                                                                                       agagTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCgg  <  2:278702/93‑1 (MQ=255)

GCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGG  >  NC_000913/2077959‑2078138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAA  >  NC_000913/2077957‑2078142

GGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGT                                                                                              <  SRR4302544.786479/94‑1 (MQ=60)

GGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGT                                                                                              <  SRR4302544.4182135/94‑1 (MQ=60)

 GGTGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTG                                                                                             <  SRR4302544.1389733/94‑1 (MQ=60)

  GCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGC                                                                                            <  SRR4302544.1694015/94‑1 (MQ=60)

   CCCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCA                                                                                           <  SRR4302544.961386/94‑1 (MQ=60)

     CAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGA                                                                                         >  SRR4302544.2716138/1‑94 (MQ=60)

      AATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAT                                                                                        <  SRR4302544.3468208/94‑1 (MQ=60)

      AATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAT                                                                                        <  SRR4302544.3468207/94‑1 (MQ=60)

       ATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATT                                                                                       >  SRR4302544.602342/1‑94 (MQ=60)

       ATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCAAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATT                                                                                       <  SRR4302544.3122914/94‑1 (MQ=60)

        TCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTG                                                                                      <  SRR4302544.4476970/94‑1 (MQ=60)

        TCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTG                                                                                      <  SRR4302544.3336353/94‑1 (MQ=60)

        TCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTG                                                                                      <  SRR4302544.2935849/94‑1 (MQ=60)

        TCAGTCCACGCAGTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTG                                                                                      <  SRR4302544.4075898/94‑1 (MQ=60)

        ACAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTG                                                                                      <  SRR4302544.1121011/94‑1 (MQ=60)

         CAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGA                                                                                     <  SRR4302544.1838813/94‑1 (MQ=60)

          AGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAT                                                                                    >  SRR4302544.47791/1‑94 (MQ=60)

          AGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAT                                                                                    <  SRR4302544.1287009/94‑1 (MQ=60)

            TCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAG                                                                                  <  SRR4302544.3295601/94‑1 (MQ=60)

             CCACGCAGTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGA                                                                                 <  SRR4302544.290240/94‑1 (MQ=60)

              CACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAC                                                                                >  SRR4302544.3029772/1‑94 (MQ=60)

               ACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACC                                                                               <  SRR4302544.4736943/94‑1 (MQ=60)

                  CATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTACGTTCGGTGAGAGTGCAGATTGAgagccccgt                                                                            >  SRR4302544.1395224/1‑85 (MQ=60)

                  CATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGA                                                                            <  SRR4302544.4226344/94‑1 (MQ=60)

                  CATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGA                                                                            >  SRR4302544.1395223/1‑94 (MQ=60)

                      tttactgcgTCGGTTAGGAGTTTTTGTAGATGGCTTTCCATACGTGGGGGTTCGAGTAAGGTCGTGGAGAGTGCAGATTGATAGACCCGATTAT                                                                        <  SRR4302544.1160247/85‑1 (MQ=60)

                        CACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATAC                                                                      <  SRR4302544.70842/94‑1 (MQ=60)

                           GGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCG                                                                   <  SRR4302544.92442/94‑1 (MQ=60)

                                CGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAG                                                              <  SRR4302544.674246/94‑1 (MQ=60)

                                CGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAG                                                              >  SRR4302544.4557159/1‑94 (MQ=60)

                                 GGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGA                                                             >  SRR4302544.2033790/1‑94 (MQ=60)

                                  GTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGAC                                                            >  SRR4302544.1970806/1‑94 (MQ=60)

                                  GTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGAC                                                            <  SRR4302544.2691234/94‑1 (MQ=60)

                                   TCATGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACT                                                           <  SRR4302544.2096341/94‑1 (MQ=60)

                                   TCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACT                                                           >  SRR4302544.2709862/1‑94 (MQ=60)

                                     AGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTT                                                         >  SRR4302544.3532850/1‑94 (MQ=60)

                                     AGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTT                                                         <  SRR4302544.2698206/94‑1 (MQ=60)

                                        AGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCG                                                      >  SRR4302544.2658875/1‑94 (MQ=60)

                                            TTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAG                                                  >  SRR4302544.1067648/1‑94 (MQ=60)

                                               GTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGCGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTG                                               >  SRR4302544.4711976/1‑94 (MQ=60)

                                                TAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGA                                              >  SRR4302544.2218638/1‑94 (MQ=60)

                                                   ATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATG                                           <  SRR4302544.1244025/94‑1 (MQ=60)

                                                    TGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGC                                          <  SRR4302544.2643634/94‑1 (MQ=60)

                                                     GGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCG                                         <  SRR4302544.3286439/94‑1 (MQ=60)

                                                       CTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCC                                       >  SRR4302544.2189850/1‑94 (MQ=60)

                                                        TTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCA                                      >  SRR4302544.4465456/1‑94 (MQ=60)

                                                           CCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTG                                   <  SRR4302544.3533085/94‑1 (MQ=60)

                                                             AGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCGGTTGCATGCGCCAGTGGT                                 >  SRR4302544.2013081/1‑94 (MQ=60)

                                                             AGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGA                                 <  SRR4302544.1529703/94‑1 (MQ=60)

                                                             AGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGA                                 >  SRR4302544.3795736/1‑94 (MQ=60)

                                                             AGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGA                                 >  SRR4302544.3795737/1‑94 (MQ=60)

                                                               ACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATG                               <  SRR4302544.4765989/94‑1 (MQ=60)

                                                                     GCGTTCGAGTAAGTTCGGTGAGAGTGCCGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCC                         <  SRR4302544.1321553/94‑1 (MQ=60)

                                                                      CGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC                        <  SRR4302544.2688219/94‑1 (MQ=60)

                                                                       GTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCG                       >  SRR4302544.674245/1‑94 (MQ=60)

                                                                       GTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCG                       >  SRR4302544.1260861/1‑94 (MQ=60)

                                                                       GTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCG                       <  SRR4302544.3354281/94‑1 (MQ=60)

                                                                        TTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGT                      >  SRR4302544.1954151/1‑94 (MQ=60)

                                                                         TCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTT                     >  SRR4302544.2995577/1‑94 (MQ=60)

                                                                          CGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTG                    >  SRR4302544.2363234/1‑94 (MQ=60)

                                                                          CGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTG                    <  SRR4302544.3209799/94‑1 (MQ=60)

                                                                          CGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTG                    <  SRR4302544.3209798/94‑1 (MQ=60)

                                                                            AGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTG                  <  SRR4302544.2151961/94‑1 (MQ=60)

                                                                             GTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGG                 <  SRR4302544.919730/94‑1 (MQ=60)

                                                                             GTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGG                 <  SRR4302544.1876166/94‑1 (MQ=60)

                                                                              TAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGC                >  SRR4302544.2528928/1‑94 (MQ=60)

                                                                               AAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCC               <  SRR4302544.515321/94‑1 (MQ=60)

                                                                                  TTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGA            >  SRR4302544.1973772/1‑94 (MQ=60)

                                                                                    CGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATC          >  SRR4302544.4390781/1‑94 (MQ=60)

                                                                                       TGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCG       <  SRR4302544.3393530/94‑1 (MQ=60)

                                                                                         AGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGA     >  SRR4302544.3079819/1‑94 (MQ=60)

                                                                                            GTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAA  >  SRR4302544.4412945/1‑94 (MQ=60)

                                                                                            GTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAA  >  SRR4302544.3641168/1‑94 (MQ=60)

GGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAA  >  NC_000913/2077957‑2078142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`