Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,078,049 A→G F189L (TTC→CTC)  yeeA ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,078,0490AG100.0% 627.0 / NA 188F189L (TTC→CTC) yeeAhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (82/106);  total (82/106)

Only 100 of 196 total aligned reads displayed.

GCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCG  >  NC_000913/2077959‑2078137
                                                                                          |                                                                                        
gcgcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTg                                                                                        <  2:3735191/93‑1 (MQ=255)
 cgcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGc                                                                                       <  2:4812195/93‑1 (MQ=255)
 cgcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGc                                                                                       <  2:685127/93‑1 (MQ=255)
 cgcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGc                                                                                       <  2:268032/93‑1 (MQ=255)
 cgcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGc                                                                                       <  2:1512460/93‑1 (MQ=255)
  gcAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCa                                                                                      >  1:1428206/1‑93 (MQ=255)
    aaTCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGa                                                                                    >  2:4422303/1‑93 (MQ=255)
    aaTCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGa                                                                                    <  2:4175688/93‑1 (MQ=255)
    aaTCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGa                                                                                    <  2:146012/93‑1 (MQ=255)
    aaTCAGTCCACGCATTTTCACGGCATCGGTCAGGAGGTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGATCGGTGGGAGTGCAGa                                                                                    >  1:6528050/1‑93 (MQ=255)
     aTCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAt                                                                                   <  2:5022507/93‑1 (MQ=255)
      tCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAtt                                                                                  <  1:2490261/93‑1 (MQ=255)
      tCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAtt                                                                                  >  1:5439146/1‑93 (MQ=255)
         gTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAt                                                                               >  1:639675/1‑93 (MQ=255)
         gTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAt                                                                               <  2:5541373/93‑1 (MQ=255)
         gTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAt                                                                               >  1:5177061/1‑93 (MQ=255)
         gTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAt                                                                               <  1:4982556/93‑1 (MQ=255)
         gTCCACGCATCTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAt                                                                               <  2:2152413/93‑1 (MQ=255)
          tCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATa                                                                              <  1:1344857/93‑1 (MQ=255)
           ccACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAg                                                                             >  2:5121370/1‑93 (MQ=255)
            cACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGa                                                                            <  2:3508205/93‑1 (MQ=255)
            cACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGa                                                                            <  1:4712366/93‑1 (MQ=255)
            cACGCATTTTCACGGCATCGGCCAGGCGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGCTGAGAGTGCAGATTGATAGa                                                                            >  2:6390750/1‑93 (MQ=255)
             aCGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAc                                                                           >  2:6082001/1‑93 (MQ=255)
              cGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAcc                                                                          <  2:1603524/93‑1 (MQ=255)
              cGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAcc                                                                          <  2:2018904/93‑1 (MQ=255)
               gCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAccg                                                                         >  2:271833/1‑92 (MQ=255)
               gCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGGGCAGGTTGATAGAccc                                                                         >  1:104006/1‑93 (MQ=255)
                 aTTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGTCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGa                                                                       <  1:5211714/93‑1 (MQ=255)
                 aTTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCgt                                                                       >  1:3179359/1‑92 (MQ=255)
                 aTTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGa                                                                       <  2:5441125/93‑1 (MQ=255)
                 aTTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGa                                                                       <  2:4944249/93‑1 (MQ=255)
                  ttttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGCGCAGATTGATAGACCCGAt                                                                      >  1:1039744/1‑93 (MQ=255)
                     tCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATtat                                                                   >  1:4715908/1‑93 (MQ=255)
                     tCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATtat                                                                   >  2:4040228/1‑93 (MQ=255)
                      cACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATtata                                                                  >  2:5167486/1‑93 (MQ=255)
                       gcGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATAc                                                                 <  1:2438585/92‑1 (MQ=255)
                       aCGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATAc                                                                 <  2:3064426/93‑1 (MQ=255)
                        cGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACt                                                                <  2:1559492/93‑1 (MQ=255)
                             tCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTc                                                           <  2:4247993/93‑1 (MQ=255)
                               ggTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAg                                                         <  2:3941479/93‑1 (MQ=255)
                               ggTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAg                                                         >  2:4474911/1‑93 (MQ=255)
                                 tCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGAc                                                       <  1:3947107/93‑1 (MQ=255)
                                 gcAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGAc                                                       <  2:5249947/92‑1 (MQ=255)
                                  cAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACt                                                      >  2:4869460/1‑93 (MQ=255)
                                   aGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACtt                                                     >  1:857363/1‑93 (MQ=255)
                                   aGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACtt                                                     >  2:1217062/1‑93 (MQ=255)
                                   aGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACtt                                                     >  2:1287213/1‑93 (MQ=255)
                                   aGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACtt                                                     <  1:1856491/93‑1 (MQ=255)
                                      aGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTc                                                  <  2:1682398/93‑1 (MQ=255)
                                         ttttGTAGATGGCTTTCCAGACGTGGGCGTTGGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGcc                                               <  1:3349731/93‑1 (MQ=255)
                                         ttttGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGcc                                               <  2:3346708/93‑1 (MQ=255)
                                          tttGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCa                                              <  1:2189085/93‑1 (MQ=255)
                                          tttGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCa                                              >  1:4333917/1‑93 (MQ=255)
                                            tGTAGAGGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGt                                            <  2:748663/93‑1 (MQ=255)
                                             gTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGtt                                           >  2:3704383/1‑93 (MQ=255)
                                               aGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTGCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGa                                         <  2:4678604/93‑1 (MQ=255)
                                                gATGGCTTTCCAGACGCGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTGAGACTTTTCTCCAGGTGaa                                        >  1:1396646/1‑93 (MQ=255)
                                                  tGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATg                                      >  2:5402266/1‑93 (MQ=255)
                                                  tGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATg                                      >  2:6013704/1‑93 (MQ=255)
                                                  tGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATg                                      <  2:6294170/93‑1 (MQ=255)
                                                   ggCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATgc                                     >  2:645721/1‑93 (MQ=255)
                                                      tttCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGcc                                  <  2:3913929/93‑1 (MQ=255)
                                                        tCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAg                                >  2:5305307/1‑93 (MQ=255)
                                                         ccAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGt                               <  2:1753085/93‑1 (MQ=255)
                                                           aGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTgg                             >  2:4491110/1‑93 (MQ=255)
                                                            gACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGa                            <  1:2272057/93‑1 (MQ=255)
                                                            gACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGa                            >  1:3829340/1‑93 (MQ=255)
                                                            gACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGa                            >  2:4966542/1‑93 (MQ=255)
                                                             aCGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGAt                           <  1:1448291/93‑1 (MQ=255)
                                                             aCGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGAt                           >  1:6180617/1‑93 (MQ=255)
                                                             aCGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGAt                           >  1:559567/1‑93 (MQ=255)
                                                             aCGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGAt                           >  1:1293410/1‑93 (MQ=255)
                                                               gTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGa                         >  1:955519/1‑93 (MQ=255)
                                                                tGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGaa                        >  1:1517055/1‑93 (MQ=255)
                                                                 gggCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAAc                       <  2:2473049/93‑1 (MQ=255)
                                                                 gggCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAAc                       >  2:2320291/1‑93 (MQ=255)
                                                                   gCGTTCGAGTAAGTTCGGTGAGAGCGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGc                     >  1:207602/1‑93 (MQ=255)
                                                                      ttCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCg                  <  2:4502149/93‑1 (MQ=255)
                                                                      ttCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCg                  >  1:775630/1‑93 (MQ=255)
                                                                      ttCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCg                  >  1:5774721/1‑93 (MQ=255)
                                                                        cGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGtt                <  2:5683741/93‑1 (MQ=255)
                                                                         gAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGTCAGTGGATGAACGCCCGTtg               <  2:2896140/93‑1 (MQ=255)
                                                                         gAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtg               <  1:3522014/93‑1 (MQ=255)
                                                                          aGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtgt              >  1:1678788/1‑93 (MQ=255)
                                                                           gTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtgtg             <  1:4805553/93‑1 (MQ=255)
                                                                           gTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTtgtg             >  1:6080561/1‑93 (MQ=255)
                                                                           gTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGCtgtg             >  1:6665323/1‑93 (MQ=255)
                                                                             aaGTGCGGTGAGAGTTCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGc           <  2:4452121/93‑1 (MQ=255)
                                                                              aGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGcc          <  2:365252/93‑1 (MQ=255)
                                                                               gtccGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCa         <  1:4173930/90‑1 (MQ=255)
                                                                               gTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCa         >  1:4634494/1‑93 (MQ=255)
                                                                               gTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCa         >  2:2370407/1‑93 (MQ=255)
                                                                                ttCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAg        >  2:6186859/1‑93 (MQ=255)
                                                                                  cGGTGAGAGTGCATATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGAt      <  2:1682347/93‑1 (MQ=255)
                                                                                  cGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGAt      >  2:4030944/1‑93 (MQ=255)
                                                                                   ggTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATa     <  1:4485955/93‑1 (MQ=255)
                                                                                    gTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATAc    >  1:4816661/1‑93 (MQ=255)
                                                                                      gagaGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCg  >  1:6292658/1‑93 (MQ=255)
                                                                                      gagaGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCg  >  1:2565429/1‑93 (MQ=255)
                                                                                          |                                                                                        
GCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCG  >  NC_000913/2077959‑2078137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAA  >  NC_000913/2077956‑2078142
                                                                                             |                                                                                             
CGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAG                                                                                               >  SRR4302536.5882583/1‑94 (MQ=60)
CGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAG                                                                                               <  SRR4302536.5940588/94‑1 (MQ=60)
CGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAG                                                                                               <  SRR4302536.1564219/94‑1 (MQ=60)
CGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTCCGGTGAGAG                                                                                               >  SRR4302536.6150532/1‑94 (MQ=60)
 GGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGT                                                                                              <  SRR4302536.6796741/94‑1 (MQ=60)
  gacttaatcagtccagatatcatcacggtatcgttcaggtaTTTTTGTAAATGACTTTCCAGGCGTTGGCGTTCGAGTAAGTTCGGTGAGATTG                                                                                             <  SRR4302536.3885961/53‑1 (MQ=26)
  GGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTG                                                                                             <  SRR4302536.2755725/94‑1 (MQ=60)
    CGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCA                                                                                           >  SRR4302536.1470629/1‑94 (MQ=60)
     GCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAG                                                                                          <  SRR4302536.5386634/94‑1 (MQ=60)
      CAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGGTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGATCGGTGGGAGTGCAGA                                                                                         >  SRR4302536.6722485/1‑94 (MQ=60)
       AATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGAT                                                                                        <  SRR4302536.253338/94‑1 (MQ=60)
        ATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATT                                                                                       >  SRR4302536.5601814/1‑94 (MQ=60)
        ATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATT                                                                                       <  SRR4302536.5079227/94‑1 (MQ=60)
        ATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATT                                                                                       >  SRR4302536.6586391/1‑94 (MQ=60)
         TCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTG                                                                                      <  SRR4302536.2565381/94‑1 (MQ=60)
           AGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAT                                                                                    >  SRR4302536.142493/1‑94 (MQ=60)
           AGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAT                                                                                    >  SRR4302536.658555/1‑94 (MQ=60)
           AGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGAT                                                                                    >  SRR4302536.5331498/1‑94 (MQ=60)
            GTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATA                                                                                   <  SRR4302536.5132169/94‑1 (MQ=60)
             TCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAG                                                                                  <  SRR4302536.1384948/94‑1 (MQ=60)
             TCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAG                                                                                  <  SRR4302536.4536580/94‑1 (MQ=60)
             TCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAG                                                                                  >  SRR4302536.4775918/1‑94 (MQ=60)
              CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGA                                                                                 <  SRR4302536.3465421/94‑1 (MQ=60)
               CACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCCGACGTGGGCGTTCGAGTAAGTTCGGTGAGCGTGCAGATTGATAGAC                                                                                >  SRR4302536.6613227/1‑94 (MQ=60)
               CACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGAC                                                                                <  SRR4302536.4854109/94‑1 (MQ=60)
                 CGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGGGCAGGTTGATAGACCC                                                                              >  SRR4302536.106784/1‑94 (MQ=60)
                   CATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGT                                                                            >  SRR4302536.3272576/1‑94 (MQ=60)
                    ATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGTCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGAT                                                                           <  SRR4302536.5367038/94‑1 (MQ=60)
                    ATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGCGCAGATTGATAGACCCGAT                                                                           >  SRR4302536.1070622/1‑94 (MQ=60)
                     TTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATT                                                                          <  SRR4302536.4193693/94‑1 (MQ=60)
                       TTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTAT                                                                        >  SRR4302536.4857777/1‑94 (MQ=60)
                        TCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATA                                                                       >  SRR4302536.1352193/1‑94 (MQ=60)
                         CACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATAC                                                                      <  SRR4302536.6731433/94‑1 (MQ=60)
                          GCGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACT                                                                     <  SRR4302536.2512299/94‑1 (MQ=60)
                          ACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACT                                                                     <  SRR4302536.146832/94‑1 (MQ=60)
                          ACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACT                                                                     >  SRR4302536.3327781/1‑94 (MQ=60)
                                TCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCA                                                               <  SRR4302536.5359834/94‑1 (MQ=60)
                                 CGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAG                                                              >  SRR4302536.4864355/1‑94 (MQ=60)
                                    TCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACT                                                           <  SRR4302536.4064348/94‑1 (MQ=60)
                                    TCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACT                                                           >  SRR4302536.435670/1‑94 (MQ=60)
                                    TCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACT                                                           >  SRR4302536.2429948/1‑94 (MQ=60)
                                    TCAGGAGTTTGTGTAGATGGCTTTCCAGACGGGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACT                                                           <  SRR4302536.4954017/94‑1 (MQ=60)
                                     CAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTT                                                          >  SRR4302536.882562/1‑94 (MQ=60)
                                     CAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTT                                                          <  SRR4302536.155157/94‑1 (MQ=60)
                                      AGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTT                                                         <  SRR4302536.1912421/94‑1 (MQ=60)
                                        GAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTC                                                       <  SRR4302536.1766991/94‑1 (MQ=60)
                                           TTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCC                                                    <  SRR4302536.817969/94‑1 (MQ=60)
                                            TTTTGTAGATGGCTTTCCAGACGTGGGCGTTGGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCA                                                   <  SRR4302536.3449062/94‑1 (MQ=60)
                                            TTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCA                                                   <  SRR4302536.3952704/94‑1 (MQ=60)
                                            TTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCA                                                   >  SRR4302536.4463717/1‑94 (MQ=60)
                                             TTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAG                                                  <  SRR4302536.2255106/94‑1 (MQ=60)
                                               TGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTT                                                >  SRR4302536.2168434/1‑94 (MQ=60)
                                                GTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTG                                               <  SRR4302536.2268657/94‑1 (MQ=60)
                                                 TAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGA                                              >  SRR4302536.6340852/1‑94 (MQ=60)
                                                  AGATGGCTTTCCAGACGCGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTGAGACTTTTCTCCAGGTGAA                                             >  SRR4302536.1438221/1‑94 (MQ=60)
                                                   GATGGCTTTCCAGAGGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAAT                                            <  SRR4302536.1702493/94‑1 (MQ=60)
                                                     TGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGC                                          <  SRR4302536.3627586/94‑1 (MQ=60)
                                                        CTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCC                                       >  SRR4302536.642117/1‑94 (MQ=60)
                                                          TTCCAGACGTGGGAGTTCGAGTAAGTTAGGTGAGAGAGCAGATTGATAGAACCGATTATACTCGGTCAGACTTTTCGCCAGCTGAATGgcaccg                                     >  SRR4302536.4822869/1‑88 (MQ=60)
                                                             CAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGG                                  >  SRR4302536.4648448/1‑94 (MQ=60)
                                                             CAGACGTGGGCGTTCGAGTAAGTTCGGTAAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGG                                  <  SRR4302536.5323581/94‑1 (MQ=60)
                                                              AGACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGA                                 >  SRR4302536.3943401/1‑94 (MQ=60)
                                                               GACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGAT                                <  SRR4302536.2633166/94‑1 (MQ=60)
                                                               GACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGAT                                <  SRR4302536.2340892/94‑1 (MQ=60)
                                                               GACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGAT                                >  SRR4302536.573826/1‑94 (MQ=60)
                                                               GACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGAT                                >  SRR4302536.6364700/1‑94 (MQ=60)
                                                               GACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGAT                                >  SRR4302536.1332083/1‑94 (MQ=60)
                                                                ACGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATG                               <  SRR4302536.1491198/94‑1 (MQ=60)
                                                                 CGTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGA                              >  SRR4302536.983766/1‑94 (MQ=60)
                                                                  GTGGGCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAA                             >  SRR4302536.1561716/1‑94 (MQ=60)
                                                                     GGCGTTCGAGTAAGTTCGGTGAGAGCGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGC                          >  SRR4302536.213079/1‑94 (MQ=60)
                                                                      GCGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAG                                                                                  >  SRR4302536.6378642/1‑37 (MQ=60)
                                                                       CGTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC                        <  SRR4302536.6142289/94‑1 (MQ=60)
                                                                        GTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCG                       >  SRR4302536.798354/1‑94 (MQ=60)
                                                                        GTTCGAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCG                       >  SRR4302536.5946553/1‑94 (MQ=60)
                                                                            GAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGT                   <  SRR4302536.3627254/94‑1 (MQ=60)
                                                                            GAGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGT                   >  SRR4302536.1727452/1‑94 (MQ=60)
                                                                             ATTAAGTTCGGTGAGAGTGCAGATTGATAGTCCTGATTATACTCGGTCAGACTTTTCGCTAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTG                  <  SRR4302536.2718713/94‑1 (MQ=60)
                                                                             AGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTG                  <  SRR4302536.2056434/94‑1 (MQ=60)
                                                                             AGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTG                  <  SRR4302536.1447314/94‑1 (MQ=60)
                                                                             AGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTG                  >  SRR4302536.6261940/1‑94 (MQ=60)
                                                                             AGTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGCTGTG                  >  SRR4302536.6863411/1‑94 (MQ=60)
                                                                              GTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGG                 <  SRR4302536.4950194/94‑1 (MQ=60)
                                                                              GTAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGG                 >  SRR4302536.1189681/1‑94 (MQ=60)
                                                                               TAAGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGC                >  SRR4302536.5880752/1‑94 (MQ=60)
                                                                                 AGTTCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCA              >  SRR4302536.4773772/1‑94 (MQ=60)
                                                                                  GTCCGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAG             <  SRR4302536.4297922/94‑1 (MQ=60)
                                                                                     CGGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATA          <  SRR4302536.765620/94‑1 (MQ=60)
                                                                                      GGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATAC         <  SRR4302536.4620681/94‑1 (MQ=60)
                                                                                      GGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATAC         <  SRR4302536.5754497/94‑1 (MQ=60)
                                                                                      GGTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATAC         >  SRR4302536.4961614/1‑94 (MQ=60)
                                                                                       GTGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACC        <  SRR4302536.969038/94‑1 (MQ=60)
                                                                                        TGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCG       >  SRR4302536.2642612/1‑94 (MQ=60)
                                                                                        TGAGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCG       >  SRR4302536.6480146/1‑94 (MQ=60)
                                                                                          AGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGT     >  SRR4302536.4268048/1‑94 (MQ=60)
                                                                                          AGAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGT     >  SRR4302536.4181048/1‑94 (MQ=60)
                                                                                           GAGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTA    <  SRR4302536.2727229/94‑1 (MQ=60)
                                                                                            AGTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAA   <  SRR4302536.2685781/94‑1 (MQ=60)
                                                                                             GTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAA  <  SRR4302536.859317/94‑1 (MQ=60)
                                                                                             GTGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGGAAA  >  SRR4302536.3896560/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGGGCGCAATCAGTCCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAGACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAA  >  NC_000913/2077956‑2078142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑