Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,081,054 A→G T93A (ACC→GCC)  sbcB → exonuclease I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,081,0540AG98.7% 497.5 / ‑2.9 158T93A (ACC→GCC) sbcBexonuclease I
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (79/77);  total (79/79)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01

Only 100 of 178 total aligned reads displayed.

GCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGAT  >  NC_000913/2080964‑2081143
                                                                                          |                                                                                         
gCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgcc                                                                                         <  1:4170781/93‑1 (MQ=255)
  cGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgt                                                                                       <  2:1626274/93‑1 (MQ=255)
    tATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtac                                                                                     >  2:2329932/1‑93 (MQ=255)
     attaattaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtacc                                                                                    >  2:3867660/1‑93 (MQ=255)
      ttaattaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtaccg                                                                                   <  2:2831977/93‑1 (MQ=255)
         attaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAg                                                                                >  1:3902852/1‑93 (MQ=255)
          ttaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGa                                                                               <  2:4316047/93‑1 (MQ=255)
                gTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGtc                                                                         >  1:1796935/1‑92 (MQ=255)
                gTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTa                                                                         >  2:1397712/1‑93 (MQ=255)
                gTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTa                                                                         >  2:1429413/1‑93 (MQ=255)
                gTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTa                                                                         >  1:1796806/1‑93 (MQ=255)
                 tATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTAt                                                                        <  2:711892/93‑1 (MQ=255)
                   ttACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTc                                                                      <  1:823082/93‑1 (MQ=255)
                   ttACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTc                                                                      >  1:2088787/1‑93 (MQ=255)
                   ttACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTc                                                                      <  1:274833/93‑1 (MQ=255)
                     aCCCCGCAAGAAGCCCGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCCCTCGCTTTTTGCCGTACCGAAGACCTGTATTCTg                                                                    <  1:2275852/93‑1 (MQ=255)
                      ccccGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTgg                                                                   >  2:3864322/1‑93 (MQ=255)
                        ccGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGc                                                                 <  1:1059816/93‑1 (MQ=255)
                        ccGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGc                                                                 <  1:1474503/93‑1 (MQ=255)
                          gCAGGAAGCACGGGCGAAAGGAGAAAACGAAGGCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTa                                                               >  2:3941995/1‑93 (MQ=255)
                          gCAGGAAGCACGGGCGAAAGGAGAAAAAGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTa                                                               >  2:4614829/1‑93 (MQ=255)
                           cAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTac                                                              <  1:4625816/93‑1 (MQ=255)
                           cAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTac                                                              >  1:3742193/1‑93 (MQ=255)
                             ggAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaa                                                            >  2:3479146/1‑93 (MQ=255)
                             ggAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaa                                                            >  1:1224273/1‑93 (MQ=255)
                             ggAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaa                                                            >  1:4006968/1‑93 (MQ=255)
                              gAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaac                                                           >  2:1687434/1‑93 (MQ=255)
                              gAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaac                                                           >  1:20315/1‑93 (MQ=255)
                                aGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaacac                                                         >  2:2536224/1‑92 (MQ=255)
                                 gCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAAt                                                        <  2:4041661/93‑1 (MQ=255)
                                   aCGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAtgt                                                      <  1:3418836/93‑1 (MQ=255)
                                    cGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAtgtg                                                     >  2:1081572/1‑93 (MQ=255)
                                     gggCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGc                                                    <  1:1490340/93‑1 (MQ=255)
                                        cGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGtt                                                 <  1:524534/93‑1 (MQ=255)
                                         gAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGttt                                                <  2:4194098/93‑1 (MQ=255)
                                         gAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGttt                                                >  1:3042572/1‑93 (MQ=255)
                                          aaaGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTc                                               >  1:669619/1‑93 (MQ=255)
                                           aaGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcg                                              >  2:396204/1‑93 (MQ=255)
                                            aGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcga                                             <  1:109375/93‑1 (MQ=255)
                                            aGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcga                                             >  1:4130004/1‑93 (MQ=255)
                                            aGGAGAAAACGAAGCCGCGTTTGACGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcga                                             <  2:2602983/93‑1 (MQ=255)
                                             ggAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgac                                            <  2:4645439/93‑1 (MQ=255)
                                             ggAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgac                                            >  2:3809167/1‑93 (MQ=255)
                                              gagaAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacg                                           <  2:486254/93‑1 (MQ=255)
                                              gagaAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacg                                           >  1:3988717/1‑93 (MQ=255)
                                               agaAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacga                                          >  1:1703630/1‑93 (MQ=255)
                                                gaAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacgac                                         <  2:3259814/93‑1 (MQ=255)
                                                gaAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacgac                                         >  2:3552210/1‑93 (MQ=255)
                                                  aaaCGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacgacga                                       >  1:3168572/1‑93 (MQ=255)
                                                   agcGAATCCGGGTTTGCCGCCTGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGaa                                      <  2:2971930/91‑1 (MQ=255)
                                                   aaCGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGaa                                      <  1:4428814/93‑1 (MQ=255)
                                                   aaCGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGaa                                      <  2:3357630/93‑1 (MQ=255)
                                                     cGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGt                                    <  2:1262966/93‑1 (MQ=255)
                                                      gAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTc                                   <  2:1085566/93‑1 (MQ=255)
                                                      gAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTc                                   <  1:3074313/93‑1 (MQ=255)
                                                       aaGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTca                                  >  2:3425518/1‑93 (MQ=255)
                                                        aGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTcac                                 >  1:3867288/1‑93 (MQ=255)
                                                          ccGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCacac                               <  2:552801/93‑1 (MQ=255)
                                                            gcgTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGc                             <  2:4444069/93‑1 (MQ=255)
                                                              gTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCaa                           <  1:1997417/93‑1 (MQ=255)
                                                               tttGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAAc                          <  1:2095727/93‑1 (MQ=255)
                                                               tttGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAAc                          >  2:2181966/1‑93 (MQ=255)
                                                                  gccgccCGTATGCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtt                       <  2:2292365/93‑1 (MQ=255)
                                                                   ccgccCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAttt                      >  1:3614112/1‑93 (MQ=255)
                                                                     gccCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAttttt                    >  1:1636149/1‑93 (MQ=255)
                                                                     gccCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACCTCACAttttt                    >  2:1913632/1‑93 (MQ=255)
                                                                      cccGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtttttt                   >  2:3510006/1‑93 (MQ=255)
                                                                       ccGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTa                  <  2:933796/93‑1 (MQ=255)
                                                                       ccGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTa                  <  1:557997/93‑1 (MQ=255)
                                                                        cGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAt                 >  1:3101948/1‑93 (MQ=255)
                                                                        cGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAt                 <  1:2291500/93‑1 (MQ=255)
                                                                        cGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAt                 <  2:923380/93‑1 (MQ=255)
                                                                         ggaTTCGCTCGCTTTTTGCCGTCACGAAGACCCGGATTCTTGGCTACAACAATGTGCGTTTCGACGACGAAGTCACCCGCAACATTTTTTATc                <  1:4480375/91‑1 (MQ=255)
                                                                         gTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATc                >  1:1153633/1‑93 (MQ=255)
                                                                         gTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATc                >  2:3100828/1‑93 (MQ=255)
                                                                          tATTCACTCACTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg               <  2:2099086/93‑1 (MQ=255)
                                                                           aTTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGACGTCACACGCAACATTTTTTATCGt              >  1:2652419/1‑93 (MQ=255)
                                                                            ttCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTa             >  1:1567206/1‑93 (MQ=255)
                                                                            ttCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTa             >  1:3478547/1‑93 (MQ=255)
                                                                              cACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGaa                                      >  2:891058/1‑66 (MQ=255)
                                                                              cACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGaa                                      <  1:891058/66‑1 (MQ=255)
                                                                              cACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAt           <  1:331861/93‑1 (MQ=255)
                                                                              cACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACAAGCAACATTTTTTATCGTAAt           >  2:1839182/1‑93 (MQ=255)
                                                                               ccTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtt          <  1:642966/92‑1 (MQ=255)
                                                                               aCTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtt          <  2:2283659/93‑1 (MQ=255)
                                                                               aCTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtt          <  1:3006615/93‑1 (MQ=255)
                                                                               aCTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtt          >  2:3312977/1‑93 (MQ=255)
                                                                                ctagCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTCTCGACGACCAAGTCACACGCAACAGTTTTTATCGTAAttt         >  2:413172/4‑93 (MQ=255)
                                                                                cTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAttt         >  2:1769010/1‑93 (MQ=255)
                                                                                cTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAttt         >  2:1961313/1‑93 (MQ=255)
                                                                                 tCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTc        >  1:2794186/1‑93 (MQ=255)
                                                                                 tCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTc        >  2:1721234/1‑93 (MQ=255)
                                                                                 tCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTc        <  2:704995/93‑1 (MQ=255)
                                                                                  cGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtttat       >  2:3673517/1‑91 (MQ=255)
                                                                                  cGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCt       <  1:3902469/93‑1 (MQ=255)
                                                                                   gCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTa      <  1:789732/93‑1 (MQ=255)
                                                                                   gCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTa      <  1:4173973/93‑1 (MQ=255)
                                                                                   gCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTa      <  1:4312110/93‑1 (MQ=255)
                                                                                   gCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTa      <  2:672359/93‑1 (MQ=255)
                                                                                       tttGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGAt  >  1:62322/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGAT  >  NC_000913/2080964‑2081143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTT  >  NC_000913/2080961‑2081147
                                                                                             |                                                                                             
GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTG                                                                                               <  SRR4302544.832597/94‑1 (MQ=60)
GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTG                                                                                               <  SRR4302544.4321557/94‑1 (MQ=60)
GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTG                                                                                               <  SRR4302544.4631482/94‑1 (MQ=60)
 GAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCTAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGC                                                                                              <  SRR4302544.2408976/94‑1 (MQ=60)
  AGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCC                                                                                             >  SRR4302544.113932/1‑94 (MQ=60)
  AGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCC                                                                                             >  SRR4302544.3066601/1‑94 (MQ=60)
  AGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCC                                                                                             <  SRR4302544.2904971/94‑1 (MQ=60)
   GCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCG                                                                                            >  SRR4302544.3944033/1‑94 (MQ=60)
   GCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCG                                                                                            <  SRR4302544.4212099/94‑1 (MQ=60)
    CCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGT                                                                                           <  SRR4302544.3420870/94‑1 (MQ=60)
    CCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGT                                                                                           <  SRR4302544.2909162/94‑1 (MQ=60)
       TATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACC                                                                                        <  SRR4302544.1864034/94‑1 (MQ=60)
        AATAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCG                                                                                       <  SRR4302544.3508010/94‑1 (MQ=60)
           AATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAG                                                                                    >  SRR4302544.1697388/1‑94 (MQ=60)
           AATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAG                                                                                    >  SRR4302544.3941621/1‑94 (MQ=60)
               ACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCT                                                                                <  SRR4302544.4217026/94‑1 (MQ=60)
                 CGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGT                                                                              <  SRR4302544.2628408/94‑1 (MQ=60)
                  GGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTC                                                                             >  SRR4302544.1814507/1‑94 (MQ=60)
                  GGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTA                                                                             >  SRR4302544.1814376/1‑94 (MQ=60)
                    TATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATT                                                                           >  SRR4302544.792618/1‑94 (MQ=60)
                     ATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTC                                                                          >  SRR4302544.2108890/1‑94 (MQ=60)
                      TTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCT                                                                         <  SRR4302544.277718/94‑1 (MQ=60)
                      TTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCT                                                                         <  SRR4302544.3708581/94‑1 (MQ=60)
                      TTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCT                                                                         <  SRR4302544.831131/94‑1 (MQ=60)
                       TACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTG                                                                        <  SRR4302544.1599022/94‑1 (MQ=60)
                       TAACCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTAACTCGCTCTTTGCCGTACCGAAGACCTGTATTCTG                                                                        >  SRR4302544.889112/1‑94 (MQ=60)
                        ACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGG                                                                       <  SRR4302544.2297283/94‑1 (MQ=60)
                        ACCCCGCAAGAAGCCCGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCCCTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGG                                                                       <  SRR4302544.2297282/94‑1 (MQ=60)
                         CCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGG                                                                      >  SRR4302544.532096/1‑94 (MQ=60)
                         CCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGG                                                                      <  SRR4302544.3296392/94‑1 (MQ=60)
                         CCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGG                                                                      <  SRR4302544.3145008/94‑1 (MQ=60)
                           CCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCT                                                                    <  SRR4302544.1070341/94‑1 (MQ=60)
                           CCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCT                                                                    >  SRR4302544.4742981/1‑94 (MQ=60)
                           CCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCT                                                                    <  SRR4302544.1489096/94‑1 (MQ=60)
                            CGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTA                                                                   <  SRR4302544.2285007/94‑1 (MQ=60)
                             GCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTAC                                                                  >  SRR4302544.3779374/1‑94 (MQ=60)
                              CAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACA                                                                 <  SRR4302544.4671691/94‑1 (MQ=60)
                              CAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACA                                                                 >  SRR4302544.2024127/1‑94 (MQ=60)
                               AGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAA                                                                >  SRR4302544.1236383/1‑94 (MQ=60)
                               AGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAA                                                                >  SRR4302544.4046838/1‑94 (MQ=60)
                                GGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAAC                                                               >  SRR4302544.20520/1‑94 (MQ=60)
                                AGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAAC                                                               <  SRR4302544.1202322/94‑1 (MQ=60)
                                 GAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACA                                                              >  SRR4302544.4478619/1‑94 (MQ=60)
                                  AAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAA                                                             >  SRR4302544.3817898/1‑94 (MQ=60)
                                   AGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAT                                                            <  SRR4302544.928053/94‑1 (MQ=60)
                                     CACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGT                                                          >  SRR4302544.2070643/1‑94 (MQ=60)
                                      ACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTG                                                         <  SRR4302544.4430181/94‑1 (MQ=60)
                                      ACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTG                                                         <  SRR4302544.3451523/94‑1 (MQ=60)
                                        GGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCG                                                       <  SRR4302544.1505077/94‑1 (MQ=60)
                                        GGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCG                                                       <  SRR4302544.1743365/94‑1 (MQ=60)
                                           CGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT                                                    <  SRR4302544.529724/94‑1 (MQ=60)
                                           CGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT                                                    >  SRR4302544.3071680/1‑94 (MQ=60)
                                            GAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTC                                                   >  SRR4302544.676235/1‑94 (MQ=60)
                                              AAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGA                                                 >  SRR4302544.4170946/1‑94 (MQ=60)
                                               AGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGAC                                                <  SRR4302544.110375/94‑1 (MQ=60)
                                                GGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACG                                               >  SRR4302544.4028419/1‑94 (MQ=60)
                                                 GAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGA                                              >  SRR4302544.1720438/1‑94 (MQ=60)
                                                    AAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGA                                           >  SRR4302544.3198726/1‑94 (MQ=60)
                                                     AAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAA                                          <  SRR4302544.3924921/94‑1 (MQ=60)
                                                     AAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAA                                          <  SRR4302544.604049/94‑1 (MQ=60)
                                                      tgaaccgctcttccgatcttatatcTTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAG                                         <  SRR4302544.899883/69‑1 (MQ=60)
                                                      AACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAG                                         <  SRR4302544.4473500/94‑1 (MQ=60)
                                                       ACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGT                                        <  SRR4302544.639742/94‑1 (MQ=60)
                                                        cgnancncnnTTTGCCGNCCNNNTTNACTCGCTTTTTGCCGTACCGAAGACCTGTATTNTGGGCTACAACAATGTGCGTTTCGACGACGAAGTC                                       <  SRR4302544.838154/84‑1 (MQ=60)
                                                         GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCA                                      <  SRR4302544.3103612/94‑1 (MQ=60)
                                                         GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCA                                      <  SRR4302544.823260/94‑1 (MQ=60)
                                                          AAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCAC                                     >  SRR4302544.4138158/1‑94 (MQ=60)
                                                          AAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCAC                                     >  SRR4302544.3905688/1‑94 (MQ=60)
                                                           AGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACA                                    <  SRR4302544.4180795/94‑1 (MQ=60)
                                                                 GTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAAC                              <  SRR4302544.2016809/94‑1 (MQ=60)
                                                                  TTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACA                             >  SRR4302544.4037526/1‑94 (MQ=60)
                                                                  TTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACA                             <  SRR4302544.2115895/94‑1 (MQ=60)
                                                                     GCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTT                          >  SRR4302544.3649642/1‑94 (MQ=60)
                                                                       CGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTT                        >  SRR4302544.1652253/1‑94 (MQ=60)
                                                                          CCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAT                     <  SRR4302544.86976/94‑1 (MQ=60)
                                                                          CCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAT                     >  SRR4302544.3131474/1‑94 (MQ=60)
                                                                          CCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAT                     <  SRR4302544.563454/94‑1 (MQ=60)
                                                                          CCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAAAATTTTTTAT                     >  SRR4302544.1312176/1‑94 (MQ=60)
                                                                           CGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATC                    >  SRR4302544.1165107/1‑94 (MQ=60)
                                                                           CGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATC                    <  SRR4302544.2313101/94‑1 (MQ=60)
                                                                            GGATTCGCTCGCTTTTTGCCGTCACGAAGACCCGGATTCTTGGCTACAACAATGTGCGTTTCGACGACGAAGTCACCCGCAACATTTTTTATCG                   <  SRR4302544.4525412/94‑1 (MQ=60)
                                                                             TATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGACGTCACACGCAACATTTTTTATCGT                  >  SRR4302544.2677852/1‑94 (MQ=60)
                                                                             TATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGT                  >  SRR4302544.1508125/1‑94 (MQ=60)
                                                                             TATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGT                  >  SRR4302544.2677853/1‑94 (MQ=60)
                                                                              ATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTA                 >  SRR4302544.1582738/1‑94 (MQ=60)
                                                                              ATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTA                 >  SRR4302544.3511719/1‑94 (MQ=60)
                                                                                 CACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATT              <  SRR4302544.335313/94‑1 (MQ=60)
                                                                                  CCTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTT             <  SRR4302544.649317/94‑1 (MQ=60)
                                                                                  ACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTT             <  SRR4302544.3035435/94‑1 (MQ=60)
                                                                                   CTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTC            >  SRR4302544.2821094/1‑94 (MQ=60)
                                                                                     CGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTA          <  SRR4302544.3941236/94‑1 (MQ=60)
                                                                                      GCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTAC         <  SRR4302544.797370/94‑1 (MQ=60)
                                                                                      GCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTAC         <  SRR4302544.4356053/94‑1 (MQ=60)
                                                                                      GCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTAC         <  SRR4302544.4215315/94‑1 (MQ=60)
                                                                                         TTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGAT      >  SRR4302544.62959/1‑94 (MQ=60)
                                                                                          TTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATC     <  SRR4302544.3093225/94‑1 (MQ=60)
                                                                                            TGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCT   >  SRR4302544.3627708/1‑94 (MQ=60)
                                                                                             GCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTT  <  SRR4302544.3683330/94‑1 (MQ=60)
                                                                                             GCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTT  <  SRR4302544.317857/94‑1 (MQ=60)
                                                                                             |                                                                                             
GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTT  >  NC_000913/2080961‑2081147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑