Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,081,054 A→G T93A (ACC→GCC)  sbcB → exonuclease I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,081,0540AG99.6% 718.4 / ‑4.9 224T93A (ACC→GCC) sbcBexonuclease I
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (98/125);  total (99/125)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.92e-01

Only 100 of 246 total aligned reads displayed.

GCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGAT  >  NC_000913/2080964‑2081143
                                                                                          |                                                                                         
gCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgcc                                                                                         <  2:1942494/93‑1 (MQ=255)
gCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgcc                                                                                         >  2:3306200/1‑93 (MQ=255)
  cGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgt                                                                                       <  1:4203977/93‑1 (MQ=255)
  cGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgt                                                                                       <  2:5707278/93‑1 (MQ=255)
     attaattaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtacc                                                                                    <  1:6715086/93‑1 (MQ=255)
       taattaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGa                                                                                  <  1:6467873/93‑1 (MQ=255)
        aattaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGaa                                                                                 <  1:4859910/93‑1 (MQ=255)
        aattaCCGGTATTACACCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGaa                                                                                 <  2:6651504/93‑1 (MQ=255)
         attaCCGGTATTACCCCGCAGGAAGCACGGGCGTAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAg                                                                                <  2:4880119/93‑1 (MQ=255)
         attaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAg                                                                                >  1:3408499/1‑93 (MQ=255)
         attaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAg                                                                                >  1:1377359/1‑93 (MQ=255)
          ttaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGa                                                                               <  1:5218400/93‑1 (MQ=255)
            aCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGAcc                                                                             <  2:6595652/93‑1 (MQ=255)
            aCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGAcc                                                                             <  2:5792880/93‑1 (MQ=255)
             ccGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCt                                                                            >  1:6263300/1‑93 (MQ=255)
              cGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTg                                                                           <  1:5967126/93‑1 (MQ=255)
                  aTTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTAtt                                                                       <  1:1671288/93‑1 (MQ=255)
                  aTTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTAtt                                                                       >  1:3653178/1‑93 (MQ=255)
                   ttACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTc                                                                      <  1:1080669/93‑1 (MQ=255)
                   ttACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTc                                                                      >  1:4420356/1‑93 (MQ=255)
                   ttACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACAGAAGACCTGTATTc                                                                      >  1:1132760/1‑93 (MQ=255)
                    tACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCt                                                                     >  2:3007630/1‑93 (MQ=255)
                     aCCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTg                                                                    <  2:1085853/93‑1 (MQ=255)
                      ccccGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTgg                                                                   <  1:324607/93‑1 (MQ=255)
                       cccGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTggg                                                                  >  2:3622350/1‑93 (MQ=255)
                       cccGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATCCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTggg                                                                  <  1:1382494/93‑1 (MQ=255)
                         cGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCt                                                                >  2:4889567/1‑93 (MQ=255)
                         cGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCt                                                                <  2:484231/93‑1 (MQ=255)
                          gCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTa                                                               >  1:4930910/1‑93 (MQ=255)
                            aGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTaca                                                             >  2:1256218/1‑93 (MQ=255)
                             ggAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTGACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaa                                                            >  2:3810199/1‑93 (MQ=255)
                             ggAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaa                                                            >  2:2478415/1‑93 (MQ=255)
                             ggAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaa                                                            >  1:3095174/1‑93 (MQ=255)
                             ggAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaa                                                            >  1:2756345/1‑93 (MQ=255)
                               aaGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTCGGCTacaaca                                                          >  1:1289680/1‑93 (MQ=255)
                                 gCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAt                                                        <  2:1443629/93‑1 (MQ=255)
                                 gCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAt                                                        <  1:5358627/93‑1 (MQ=255)
                                 gCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAt                                                        >  2:2805715/1‑93 (MQ=255)
                                   aCGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAtgt                                                      <  2:3331768/93‑1 (MQ=255)
                                    cGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAtgtg                                                     <  2:3183776/93‑1 (MQ=255)
                                     gggCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGc                                                    <  2:4080469/93‑1 (MQ=255)
                                      ggCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGACGACCTGTATTCTGGGCTACAACAATGTGCg                                                   >  2:5171017/1‑93 (MQ=255)
                                      ggCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCg                                                   >  2:3371028/1‑93 (MQ=255)
                                        cGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGtt                                                 <  2:4156388/93‑1 (MQ=255)
                                        cGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGtt                                                 <  2:4638922/93‑1 (MQ=255)
                                        cGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCAGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGtt                                                 <  1:801332/93‑1 (MQ=255)
                                         gAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGttt                                                <  2:1690558/93‑1 (MQ=255)
                                         gAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGttt                                                >  1:3752565/1‑93 (MQ=255)
                                         gAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGttt                                                >  2:3682313/1‑93 (MQ=255)
                                          aaaGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTc                                               <  1:2378009/93‑1 (MQ=255)
                                          aaaGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTc                                               <  1:4694604/93‑1 (MQ=255)
                                           aaGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcg                                              >  2:2238613/1‑93 (MQ=255)
                                            aGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcga                                             <  1:4295147/93‑1 (MQ=255)
                                             ggAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgac                                            <  2:1168635/93‑1 (MQ=255)
                                                gaAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacgac                                         <  2:208428/93‑1 (MQ=255)
                                                    aCGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAg                                     >  2:3248235/1‑93 (MQ=255)
                                                    aCGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAg                                     <  2:2654656/93‑1 (MQ=255)
                                                     cGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGt                                    <  2:621055/93‑1 (MQ=255)
                                                      gAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTc                                   <  1:4968883/93‑1 (MQ=255)
                                                       aaGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGTCGAAGTca                                  <  1:6526896/93‑1 (MQ=255)
                                                        aGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTcac                                 >  2:4951434/1‑93 (MQ=255)
                                                        aGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTcac                                 >  2:2334269/1‑93 (MQ=255)
                                                        aGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTcac                                 >  1:5338332/1‑93 (MQ=255)
                                                         gCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTcaca                                <  1:3916928/93‑1 (MQ=255)
                                                              gTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCaa                           >  1:5609382/1‑93 (MQ=255)
                                                              gTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCaa                           <  1:5581720/93‑1 (MQ=255)
                                                               tttGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAAc                          <  2:5873903/93‑1 (MQ=255)
                                                                  gccgccCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtt                       <  2:5149145/93‑1 (MQ=255)
                                                                    cgccCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtttt                     <  1:6116913/93‑1 (MQ=255)
                                                                      cccGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtttttt                   <  1:568202/93‑1 (MQ=255)
                                                                       ccGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTa                  >  2:1006893/1‑93 (MQ=255)
                                                                       ccGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTa                  >  1:2916620/1‑93 (MQ=255)
                                                                        cGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAt                 <  2:1773854/93‑1 (MQ=255)
                                                                        cGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAt                 >  2:6638728/1‑93 (MQ=255)
                                                                        cGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAt                 <  2:3944847/93‑1 (MQ=255)
                                                                         gTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATc                >  2:3071974/1‑93 (MQ=255)
                                                                          tATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg               <  2:1393962/93‑1 (MQ=255)
                                                                          tATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg               >  1:221827/1‑93 (MQ=255)
                                                                          tATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg               <  1:2634620/93‑1 (MQ=255)
                                                                          tATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg               <  1:5522703/93‑1 (MQ=255)
                                                                           aTTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGt              >  2:3555075/1‑93 (MQ=255)
                                                                           aTTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGt              <  1:2786704/93‑1 (MQ=255)
                                                                             tCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTaa            <  2:2000267/93‑1 (MQ=255)
                                                                              cACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAt           >  2:93913/1‑93 (MQ=255)
                                                                              cACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAt           <  2:6315863/93‑1 (MQ=255)
                                                                              cACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAt           <  2:3847026/93‑1 (MQ=255)
                                                                              cACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAt           <  1:3553172/93‑1 (MQ=255)
                                                                               aCTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtt          >  2:3170952/1‑93 (MQ=255)
                                                                               aCTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtt          <  1:2328403/93‑1 (MQ=255)
                                                                                cTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAttt         >  2:5295581/1‑93 (MQ=255)
                                                                                 tCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTc        <  1:360441/93‑1 (MQ=255)
                                                                                  cGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCt       <  1:4731735/93‑1 (MQ=255)
                                                                                  cGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCt       >  2:2174557/1‑93 (MQ=255)
                                                                                   gCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTa      >  2:2414165/1‑93 (MQ=255)
                                                                                    cTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTAc     <  1:6017670/93‑1 (MQ=255)
                                                                                    cTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTAc     <  2:6438738/93‑1 (MQ=255)
                                                                                     tttttGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACg    >  2:6181083/1‑93 (MQ=255)
                                                                                     tttttGCCGTACCGAAGACATGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACg    <  2:3996179/93‑1 (MQ=255)
                                                                                      ttttGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGa   <  1:1509238/93‑1 (MQ=255)
                                                                                       tttGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGAt  <  1:6687799/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGAT  >  NC_000913/2080964‑2081143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTT  >  NC_000913/2080961‑2081147
                                                                                             |                                                                                             
GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTG                                                                                               <  SRR4302536.5647712/94‑1 (MQ=60)
GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTG                                                                                               >  SRR4302536.3807138/1‑94 (MQ=60)
GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTG                                                                                               <  SRR4302536.3900930/94‑1 (MQ=60)
   GCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCG                                                                                            <  SRR4302536.1419866/94‑1 (MQ=60)
    CCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGT                                                                                           <  SRR4302536.6663424/94‑1 (MQ=60)
     CGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTA                                                                                          >  SRR4302536.5437890/1‑94 (MQ=60)
     CGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTA                                                                                          >  SRR4302536.762654/1‑94 (MQ=60)
     CGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTA                                                                                          <  SRR4302536.4328940/94‑1 (MQ=60)
      GTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTAC                                                                                         >  SRR4302536.3724292/1‑94 (MQ=60)
      GTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTAC                                                                                         >  SRR4302536.248769/1‑94 (MQ=60)
       TATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACC                                                                                        >  SRR4302536.5999053/1‑94 (MQ=60)
        ATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCG                                                                                       <  SRR4302536.6914430/94‑1 (MQ=60)
          TAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAA                                                                                     <  SRR4302536.6660924/94‑1 (MQ=60)
          TAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAAGAAGCCGCGTTTGCCGCCCGTAGTCACTCGCTTTTTGCCGTACCGAA                                                                                     >  SRR4302536.5026206/1‑94 (MQ=60)
           AATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAG                                                                                    >  SRR4302536.1418385/1‑94 (MQ=60)
           AATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAG                                                                                    >  SRR4302536.3509884/1‑94 (MQ=60)
           AATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAG                                                                                    <  SRR4302536.5006164/94‑1 (MQ=60)
            ATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGA                                                                                   >  SRR4302536.689454/1‑94 (MQ=60)
             TTACCGGTATTCCCCCGCAGGAAGCGCGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGAC                                                                                  <  SRR4302536.3697853/94‑1 (MQ=60)
             TTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGAC                                                                                  <  SRR4302536.5373896/94‑1 (MQ=60)
               ACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCT                                                                                >  SRR4302536.6449894/1‑94 (MQ=60)
                CCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTG                                                                               <  SRR4302536.5540115/94‑1 (MQ=60)
                 CGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGT                                                                              <  SRR4302536.6144968/94‑1 (MQ=60)
                  GGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTA                                                                             >  SRR4302536.3819727/1‑94 (MQ=60)
                    TATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATT                                                                           >  SRR4302536.3762392/1‑94 (MQ=60)
                    TAATACCCCGCTGGAAGGACGGGCGATAGGAGAAAACGAAGCCGCGTTTGCCGCGCGTATTCGCTCGCTTTTTGCCGTACCGAAGACCTGTATT                                                                           <  SRR4302536.4610299/94‑1 (MQ=60)
                     ATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTC                                                                          >  SRR4302536.4552723/1‑94 (MQ=60)
                     ATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTC                                                                          <  SRR4302536.1719763/94‑1 (MQ=60)
                     ATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACAGAAGACCTGTATTC                                                                          >  SRR4302536.1166822/1‑94 (MQ=60)
                      TTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCT                                                                         <  SRR4302536.1113026/94‑1 (MQ=60)
                       TACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTG                                                                        <  SRR4302536.1360907/94‑1 (MQ=60)
                       TACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTG                                                                        <  SRR4302536.4541796/94‑1 (MQ=60)
                        ACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGG                                                                       >  SRR4302536.2425900/1‑94 (MQ=60)
                         CCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGG                                                                      <  SRR4302536.333216/94‑1 (MQ=60)
                          CCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATCCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGC                                                                     <  SRR4302536.1423672/94‑1 (MQ=60)
                           CCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCT                                                                    <  SRR4302536.4139757/94‑1 (MQ=60)
                            CGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTA                                                                   <  SRR4302536.5756683/94‑1 (MQ=60)
                            CGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTA                                                                   >  SRR4302536.5079062/1‑94 (MQ=60)
                              CAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACA                                                                 >  SRR4302536.4554519/1‑94 (MQ=60)
                               NNGAANCACGGGCGAAAGGAGAAAACNAAGCCGCGTTTGNCGCCCGTTTNCACTNNCTNTNNGNCGTACCGAAGACCTGTATNCTGGGCTACAA                                                                >  SRR4302536.5573532/1‑94 (MQ=60)
                               AGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAC                                                                >  SRR4302536.2364356/1‑94 (MQ=60)
                               AGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAA                                                                >  SRR4302536.3185607/1‑94 (MQ=60)
                               AGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAA                                                                >  SRR4302536.2838387/1‑94 (MQ=60)
                                 GAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACA                                                              <  SRR4302536.5498922/94‑1 (MQ=60)
                                 GAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACA                                                              <  SRR4302536.3410621/94‑1 (MQ=60)
                                 GAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTCGGCTACAACA                                                              >  SRR4302536.1328259/1‑94 (MQ=60)
                                  AAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAT                                                             >  SRR4302536.3459067/1‑94 (MQ=60)
                                    GCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATG                                                           <  SRR4302536.5518516/94‑1 (MQ=60)
                                    GCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATG                                                           <  SRR4302536.1432835/94‑1 (MQ=60)
                                    GCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATG                                                           >  SRR4302536.5760208/1‑94 (MQ=60)
                                      ACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTG                                                         >  SRR4302536.3794687/1‑94 (MQ=60)
                                      ACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTG                                                         <  SRR4302536.6696963/94‑1 (MQ=60)
                                       CGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGC                                                        >  SRR4302536.6910747/1‑94 (MQ=60)
                                        GGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCG                                                       <  SRR4302536.2734444/94‑1 (MQ=60)
                                        GGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCG                                                       <  SRR4302536.4278905/94‑1 (MQ=60)
                                           CGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT                                                    <  SRR4302536.1283999/94‑1 (MQ=60)
                                           CGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT                                                    >  SRR4302536.397393/1‑94 (MQ=60)
                                           CGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT                                                    >  SRR4302536.3864688/1‑94 (MQ=60)
                                           CGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCAGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT                                                    <  SRR4302536.824825/94‑1 (MQ=60)
                                            GAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTC                                                   <  SRR4302536.5080599/94‑1 (MQ=60)
                                             AAATGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCG                                                  <  SRR4302536.1029070/94‑1 (MQ=60)
                                             AAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCG                                                  <  SRR4302536.2450044/94‑1 (MQ=60)
                                             AAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCG                                                  <  SRR4302536.4835775/94‑1 (MQ=60)
                                             AAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCG                                                  >  SRR4302536.3678264/1‑94 (MQ=60)
                                              AAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGA                                                 >  SRR4302536.2944846/1‑94 (MQ=60)
                                              AAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGA                                                 >  SRR4302536.4860524/1‑94 (MQ=60)
                                               AGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGAC                                                <  SRR4302536.4423512/94‑1 (MQ=60)
                                                    AAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGA                                           >  SRR4302536.950483/1‑94 (MQ=60)
                                                    AAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGA                                           >  SRR4302536.4351310/1‑94 (MQ=60)
                                                    AAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGA                                           >  SRR4302536.1132750/1‑94 (MQ=60)
                                                      AACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAG                                         <  SRR4302536.5729631/94‑1 (MQ=60)
                                                       ACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGT                                        >  SRR4302536.3160700/1‑94 (MQ=60)
                                                        CGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTC                                       >  SRR4302536.3182869/1‑94 (MQ=60)
                                                        CGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTC                                       <  SRR4302536.5112015/94‑1 (MQ=60)
                                                         GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCA                                      >  SRR4302536.546052/1‑94 (MQ=60)
                                                         GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCA                                      <  SRR4302536.5118144/94‑1 (MQ=60)
                                                         GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCA                                      >  SRR4302536.1764195/1‑94 (MQ=60)
                                                          AAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGTCGAAGTCAC                                     <  SRR4302536.6721302/94‑1 (MQ=60)
                                                          AAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCAC                                     >  SRR4302536.5497808/1‑94 (MQ=60)
                                                            GCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACAC                                   <  SRR4302536.4033370/94‑1 (MQ=60)
                                                            GCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACAC                                   <  SRR4302536.5672719/94‑1 (MQ=60)
                                                               GCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCA                                >  SRR4302536.6140472/1‑94 (MQ=60)
                                                                CGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAA                               >  SRR4302536.5776043/1‑94 (MQ=60)
                                                                 GTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAAC                              <  SRR4302536.5747823/94‑1 (MQ=60)
                                                                   TTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAT                            <  SRR4302536.2851897/94‑1 (MQ=60)
                                                                   TTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAT                            <  SRR4302536.2432533/94‑1 (MQ=60)
                                                                   TTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAT                            <  SRR4302536.1331208/94‑1 (MQ=60)
                                                                   TTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACCACGAAGTCACACGCAACAT                            <  SRR4302536.5354502/94‑1 (MQ=60)
                                                                    TGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATT                           >  SRR4302536.4968252/1‑94 (MQ=60)
                                                                    TGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATT                           >  SRR4302536.5845617/1‑94 (MQ=60)
                                                                       CGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTT                        <  SRR4302536.6299236/94‑1 (MQ=60)
                                                                        GCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTT                       <  SRR4302536.6834769/94‑1 (MQ=60)
                                                                        CCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTT                       <  SRR4302536.6365779/94‑1 (MQ=60)
                                                                         CCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTA                      >  SRR4302536.3002708/1‑94 (MQ=60)
                                                                         CCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTA                      <  SRR4302536.3564634/94‑1 (MQ=60)
                                                                         CCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTA                      >  SRR4302536.2005027/1‑94 (MQ=60)
                                                                         CCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTA                      <  SRR4302536.582669/94‑1 (MQ=60)
                                                                           CGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATC                    >  SRR4302536.279141/1‑94 (MQ=60)
                                                                           CGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATC                    >  SRR4302536.1543099/1‑94 (MQ=60)
                                                                            GTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCG                   >  SRR4302536.157465/1‑94 (MQ=60)
                                                                            GTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCG                   >  SRR4302536.227709/1‑94 (MQ=60)
                                                                            GTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGCTTCGACGACGAAGTCACACGCAACATTTTTTATCG                   >  SRR4302536.653437/1‑94 (MQ=60)
                                                                             TATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGT                  <  SRR4302536.5687378/94‑1 (MQ=60)
                                                                             TATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGT                  <  SRR4302536.2713632/94‑1 (MQ=60)
                                                                              ATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTA                 <  SRR4302536.2869460/94‑1 (MQ=60)
                                                                                TCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAT               >  SRR4302536.4550681/1‑94 (MQ=60)
                                                                                 CACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATT              <  SRR4302536.3659404/94‑1 (MQ=60)
                                                                                  ACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTT             <  SRR4302536.2399065/94‑1 (MQ=60)
                                                                                   CTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTT            >  SRR4302536.6277159/1‑94 (MQ=60)
                                                                                    TCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCT           >  SRR4302536.6337837/1‑94 (MQ=60)
                                                                                    TCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCT           <  SRR4302536.3198911/94‑1 (MQ=60)
                                                                                    TCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCT           <  SRR4302536.369969/94‑1 (MQ=60)
                                                                                     CGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTA          <  SRR4302536.4874118/94‑1 (MQ=60)
                                                                                       CTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACG        >  SRR4302536.614158/1‑94 (MQ=60)
                                                                                       CTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACG        <  SRR4302536.6197084/94‑1 (MQ=60)
                                                                                         TTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGAT      <  SRR4302536.1553710/94‑1 (MQ=60)
                                                                                         TTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGAT      <  SRR4302536.4891126/94‑1 (MQ=60)
                                                                                          TTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATC     >  SRR4302536.2466190/1‑94 (MQ=60)
                                                                                          TTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATC     <  SRR4302536.6886422/94‑1 (MQ=60)
                                                                                            TGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCT   <  SRR4302536.4727781/94‑1 (MQ=60)
                                                                                            TGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCAT   >  SRR4302536.2150917/1‑94 (MQ=60)
                                                                                             GCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTT  <  SRR4302536.1665210/94‑1 (MQ=60)
                                                                                             GCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTT  >  SRR4302536.6450661/1‑94 (MQ=60)
                                                                                             GCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTT  <  SRR4302536.5132251/94‑1 (MQ=60)
                                                                                             |                                                                                             
GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTT  >  NC_000913/2080961‑2081147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑