Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,111,589	A→G	M254T (ATG→ACG)	galF ←	putative a UDP‑gal transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,111,589	0	A	G	100.0%	568.6 / NA	171	M254T (ATG→ACG)	galF	putative a UDP‑gal transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (62/109); total (62/109)

Only 100 of 179 total aligned reads displayed.

CCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGT  >  NC_000913/2111499‑2111678

ccTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGtt                                                                                         <  2:2679323/93‑1 (MQ=255)

ccTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGtt                                                                                         <  2:2016660/93‑1 (MQ=255)

ccTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGtt                                                                                         <  1:651348/93‑1 (MQ=255)

ccTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGtt                                                                                         <  1:4513885/93‑1 (MQ=255)

  ttcttTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCGGGTCATCAGCGTTga                                                                                       >  2:739011/1‑92 (MQ=255)

  ttcttTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGc                                                                                       <  2:3642942/93‑1 (MQ=255)

   tcttTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCa                                                                                      <  2:2728657/93‑1 (MQ=255)

    cttTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCAt                                                                                     <  2:1233174/93‑1 (MQ=255)

    cttTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGACCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCAt                                                                                     >  1:2098050/1‑93 (MQ=255)

     tttCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATc                                                                                    >  1:3471739/1‑93 (MQ=255)

      ttCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCa                                                                                   >  2:3244335/1‑93 (MQ=255)

      ttCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCa                                                                                   >  2:342143/1‑93 (MQ=255)

       tCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCaa                                                                                  <  1:841101/93‑1 (MQ=255)

        cAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAAc                                                                                 >  2:3044192/1‑93 (MQ=255)

         aGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACg                                                                                <  1:1431308/93‑1 (MQ=255)

         aGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACg                                                                                <  1:251237/93‑1 (MQ=255)

          ggTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACgg                                                                               <  1:2133977/93‑1 (MQ=255)

           gTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGa                                                                              <  2:2650769/93‑1 (MQ=255)

           gTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGa                                                                              >  2:4680574/1‑93 (MQ=255)

            ttGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGAt                                                                             >  1:477965/1‑93 (MQ=255)

            ttGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGAt                                                                             <  2:1698285/93‑1 (MQ=255)

             tGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGAtt                                                                            <  1:3773189/93‑1 (MQ=255)

             tGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGAtt                                                                            <  1:3410787/93‑1 (MQ=255)

                gTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTACCTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGtt                                                                         <  2:4622650/93‑1 (MQ=255)

                gTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGtt                                                                         <  1:3316716/93‑1 (MQ=255)

                gTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGtt                                                                         >  2:3228691/1‑93 (MQ=255)

                 tAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGttt                                                                        <  2:2789573/93‑1 (MQ=255)

                  aGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGtttt                                                                       <  1:3819791/93‑1 (MQ=255)

                   ggCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGttttt                                                                      <  1:299911/93‑1 (MQ=255)

                     ccATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTc                                                                    <  1:2217275/93‑1 (MQ=255)

                     ccATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTc                                                                    <  2:88880/93‑1 (MQ=255)

                      cATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCg                                                                   >  2:1978857/1‑93 (MQ=255)

                      cATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCg                                                                   >  2:564893/1‑93 (MQ=255)

                         aCTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCa                                                                <  1:3088174/93‑1 (MQ=255)

                           ttCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGc                                                              >  1:2587135/1‑93 (MQ=255)

                           ttCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGc                                                              <  1:683676/93‑1 (MQ=255)

                            tCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCt                                                             <  2:2156403/93‑1 (MQ=255)

                             cacaAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTc                                                            <  2:3912448/93‑1 (MQ=255)

                              acaAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCg                                                           <  2:1853316/93‑1 (MQ=255)

                              acaAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCg                                                           <  1:2255480/93‑1 (MQ=255)

                                aaaCGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGc                                                         <  1:1698944/93‑1 (MQ=255)

                                aaaCGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGc                                                         <  1:1472180/93‑1 (MQ=255)

                                  aCGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCaa                                                       >  2:2768049/1‑93 (MQ=255)

                                  aCGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCaa                                                       <  1:2737165/93‑1 (MQ=255)

                                    gCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATa                                                     <  2:2704322/93‑1 (MQ=255)

                                    gCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATa                                                     >  2:1109874/1‑93 (MQ=255)

                                       tGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCa                                                  <  2:1222938/93‑1 (MQ=255)

                                         gatatAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtc                                                <  2:2965224/92‑1 (MQ=255)

                                          atatAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtca                                               <  1:3987418/93‑1 (MQ=255)

                                          atatAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtca                                               <  1:1119172/93‑1 (MQ=255)

                                           tataGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcag                                              >  1:1171815/1‑93 (MQ=255)

                                             taGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcagtc                                            <  1:4032791/93‑1 (MQ=255)

                                              aGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcagtca                                           <  2:402976/93‑1 (MQ=255)

                                              aGCCCATTTTTTTGCCGCAGGCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcagtca                                           <  1:2347792/93‑1 (MQ=255)

                                               gCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcagtcag                                          >  1:1637454/1‑93 (MQ=255)

                                               gCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcagtcag                                          >  1:147064/1‑93 (MQ=255)

                                                cccATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGc                                         <  1:3661251/93‑1 (MQ=255)

                                                 ccATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCt                                        <  2:1695389/93‑1 (MQ=255)

                                                 ccATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCt                                        >  1:1883057/1‑93 (MQ=255)

                                                 ccATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCt                                        >  2:85583/1‑93 (MQ=255)

                                                  cATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTg                                       >  1:4606174/1‑93 (MQ=255)

                                                  cATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTg                                       >  2:795757/1‑93 (MQ=255)

                                                    tttttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGaa                                     <  1:688329/93‑1 (MQ=255)

                                                    tttttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGaa                                     <  1:957247/93‑1 (MQ=255)

                                                    tttttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTAGCCAGCTCGGCAATAGCATCAGTCAGTTGaa                                     >  1:4092645/1‑93 (MQ=255)

                                                     ttttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAAt                                    >  1:858416/1‑93 (MQ=255)

                                                     ttttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAAt                                    <  2:3675421/93‑1 (MQ=255)

                                                      tttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATa                                   <  2:4384362/93‑1 (MQ=255)

                                                      tttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATa                                   <  2:4598952/93‑1 (MQ=255)

                                                      tttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATa                                   <  2:1649250/93‑1 (MQ=255)

                                                      tttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATa                                   <  2:258715/93‑1 (MQ=255)

                                                       ttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATAc                                  <  1:1806887/93‑1 (MQ=255)

                                                        tttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACg                                 >  2:2975560/1‑93 (MQ=255)

                                                         ttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGt                                <  1:3415853/93‑1 (MQ=255)

                                                           gCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTcc                              >  2:502424/1‑93 (MQ=255)

                                                            ccGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTccc                             <  2:4726962/93‑1 (MQ=255)

                                                              gCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCa                           <  2:4558441/93‑1 (MQ=255)

                                                              gCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCa                           <  2:3493559/93‑1 (MQ=255)

                                                               cAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCac                          <  2:2536963/93‑2 (MQ=255)

                                                                aGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCacg                         >  1:3205049/1‑91 (MQ=255)

                                                                aGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCacg                         >  1:3205048/1‑91 (MQ=255)

                                                                  tCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCa                       >  2:3667027/1‑93 (MQ=255)

                                                                   cGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCAc                      <  2:1362133/93‑1 (MQ=255)

                                                                    gTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCAcc                     >  2:1733716/1‑93 (MQ=255)

                                                                    gTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCAcc                     <  2:733607/93‑1 (MQ=255)

                                                                     tAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCa                    >  2:3228194/1‑93 (MQ=255)

                                                                     tAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCa                    <  2:3145312/93‑1 (MQ=255)

                                                                     tAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCa                    >  1:4254074/1‑93 (MQ=255)

                                                                        cTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGc                 <  1:3425725/93‑1 (MQ=255)

                                                                        cTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGc                 >  2:1714688/1‑93 (MQ=255)

                                                                          gTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTg               >  1:1572611/1‑93 (MQ=255)

                                                                           tCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGa              <  2:2134063/93‑1 (MQ=255)

                                                                            cGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAg             <  2:1740972/93‑1 (MQ=255)

                                                                             gCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCCGGCTGAGt            >  1:4304986/1‑93 (MQ=255)

                                                                             gCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGt            >  1:317142/1‑93 (MQ=255)

                                                                               cGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAc          <  2:568897/93‑1 (MQ=255)

                                                                                   catcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTc      <  1:1314272/93‑1 (MQ=255)

                                                                                    atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc     >  1:2980763/1‑93 (MQ=255)

                                                                                    atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc     <  2:662641/93‑1 (MQ=255)

                                                                                    atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc     <  2:1775124/93‑1 (MQ=255)

CCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGT  >  NC_000913/2111499‑2111678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTT  >  NC_000913/2111497‑2111679

CCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGT                                                                                           >  SRR4302544.1558384/1‑94 (MQ=60)

  CCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTG                                                                                         <  SRR4302544.4095187/94‑1 (MQ=60)

  CCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTG                                                                                         <  SRR4302544.4559102/94‑1 (MQ=60)

  CCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTG                                                                                         <  SRR4302544.657796/94‑1 (MQ=60)

     TCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGACCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCAT                                                                                      >  SRR4302544.2118236/1‑94 (MQ=60)

      CTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATC                                                                                     >  SRR4302544.3504848/1‑94 (MQ=60)

      CTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATC                                                                                     <  SRR4302544.1480178/94‑1 (MQ=60)

       TTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCA                                                                                    >  SRR4302544.962639/1‑94 (MQ=60)

         TCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAAC                                                                                  <  SRR4302544.849400/94‑1 (MQ=60)

           AGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGG                                                                                <  SRR4302544.1445549/94‑1 (MQ=60)

           AGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGG                                                                                <  SRR4302544.253878/94‑1 (MQ=60)

            GGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGA                                                                               <  SRR4302544.2154393/94‑1 (MQ=60)

             GTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGAT                                                                              >  SRR4302544.482646/1‑94 (MQ=60)

              TTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATT                                                                             <  SRR4302544.3983069/94‑1 (MQ=60)

               TGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTG                                                                            <  SRR4302544.1613071/94‑1 (MQ=60)

               TGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTG                                                                            <  SRR4302544.3810585/94‑1 (MQ=60)

               TGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTG                                                                            <  SRR4302544.3443392/94‑1 (MQ=60)

                  GTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTT                                                                         <  SRR4302544.3348314/94‑1 (MQ=60)

                    AGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTT                                                                       <  SRR4302544.3857656/94‑1 (MQ=60)

                     GGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTT                                                                      >  SRR4302544.3190927/1‑94 (MQ=60)

                     GGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTT                                                                      <  SRR4302544.303014/94‑1 (MQ=60)

                      GCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTC                                                                     <  SRR4302544.4695047/94‑1 (MQ=60)

                      GCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTC                                                                     <  SRR4302544.3208627/94‑1 (MQ=60)

                       CCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCG                                                                    <  SRR4302544.3660132/94‑1 (MQ=60)

                       CCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCG                                                                    <  SRR4302544.2238130/94‑1 (MQ=60)

                         ATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCC                                                                  <  SRR4302544.4366621/94‑1 (MQ=60)

                           ACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAG                                                                >  SRR4302544.2835574/1‑94 (MQ=60)

                           ACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAG                                                                <  SRR4302544.3117597/94‑1 (MQ=60)

                            CTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGC                                                               >  SRR4302544.2612020/1‑94 (MQ=60)

                             TTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCT                                                              <  SRR4302544.690474/94‑1 (MQ=60)

                               CACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCG                                                            >  SRR4302544.2606870/1‑94 (MQ=60)

                                ACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGG                                                           <  SRR4302544.2276699/94‑1 (MQ=60)

                                 CAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGC                                                          >  SRR4302544.2141352/1‑94 (MQ=60)

                                  AAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCA                                                         <  SRR4302544.1715711/94‑1 (MQ=60)

                                  AAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCA                                                         <  SRR4302544.1486759/94‑1 (MQ=60)

                                    ACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAAT                                                       <  SRR4302544.1197896/94‑1 (MQ=60)

                                    ACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAAT                                                       <  SRR4302544.3280116/94‑1 (MQ=60)

                                    ACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAAT                                                       <  SRR4302544.2763550/94‑1 (MQ=60)

                                           CATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCA                                                <  SRR4302544.849675/94‑1 (MQ=60)

                                            ATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAG                                               >  SRR4302544.1183503/1‑94 (MQ=60)

                                            ATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAG                                               <  SRR4302544.4027112/94‑1 (MQ=60)

                                            ATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAG                                               <  SRR4302544.1130335/94‑1 (MQ=60)

                                              ATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTC                                             >  SRR4302544.1415533/1‑94 (MQ=60)

                                               TAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCA                                            <  SRR4302544.4072963/94‑1 (MQ=60)

                                                AGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAG                                           >  SRR4302544.148470/1‑94 (MQ=60)

                                                AGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAG                                           <  SRR4302544.665011/94‑1 (MQ=60)

                                                AGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAG                                           >  SRR4302544.1653579/1‑94 (MQ=60)

                                                AGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAG                                           >  SRR4302544.43379/1‑94 (MQ=60)

                                                AGCCCATTTTTTTGCCGCAGGCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAG                                           <  SRR4302544.2370033/94‑1 (MQ=60)

                                                 GCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGC                                          <  SRR4302544.2464032/94‑1 (MQ=60)

                                                  CCCATTTTTTTTCCGCAGTCGTAACTGTCTCCGTTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCT                                         <  SRR4302544.1493845/94‑1 (MQ=60)

                                                  CCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCT                                         >  SRR4302544.1901258/1‑94 (MQ=60)

                                                  CCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCT                                         <  SRR4302544.3697772/94‑1 (MQ=60)

                                                  CCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCT                                         <  SRR4302544.820784/94‑1 (MQ=60)

                                                   CCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTG                                        >  SRR4302544.4651939/1‑94 (MQ=60)

                                                     ATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAA                                      >  SRR4302544.519645/1‑94 (MQ=60)

                                                     ATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTAGCCAGCTCGGCAATAGCATCAGTCAGTTGAA                                      >  SRR4302544.4133360/1‑94 (MQ=60)

                                                      TTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAAT                                     <  SRR4302544.695180/94‑1 (MQ=60)

                                                      TTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAAT                                     >  SRR4302544.866906/1‑94 (MQ=60)

                                                      TTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAAT                                     <  SRR4302544.966655/94‑1 (MQ=60)

                                                       TTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATA                                    <  SRR4302544.3779652/94‑1 (MQ=60)

                                                        TTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATAC                                   <  SRR4302544.1985616/94‑1 (MQ=60)

                                                         TTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACG                                  <  SRR4302544.1824545/94‑1 (MQ=60)

                                                           TTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTC                                <  SRR4302544.3448500/94‑1 (MQ=60)

                                                           TTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTC                                <  SRR4302544.2235932/94‑1 (MQ=60)

                                                           TTGCCGCAGGCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTC                                <  SRR4302544.3448499/94‑1 (MQ=60)

                                                                 CAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACG                          >  SRR4302544.3235612/1‑94 (MQ=60)

                                                                 CAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACG                          >  SRR4302544.3235613/1‑94 (MQ=60)

                                                                      GTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCA                     >  SRR4302544.4297446/1‑94 (MQ=60)

                                                                      GTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCA                     <  SRR4302544.3416649/94‑1 (MQ=60)

                                                                       TAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTTTTTTTTCGCGAGCTCGGCAATAGCATCAGTCAGGTGAATAggtgcacacccactag                    >  SRR4302544.270732/1‑78 (MQ=60)

                                                                       TAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAG                    >  SRR4302544.4158285/1‑94 (MQ=60)

                                                                         ACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGC                  >  SRR4302544.3116966/1‑94 (MQ=60)

                                                                          CTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCT                 <  SRR4302544.3458477/94‑1 (MQ=60)

                                                                           TGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTG                >  SRR4302544.1588160/1‑94 (MQ=60)

                                                                              CGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCCGGCTGAGT             >  SRR4302544.4348892/1‑94 (MQ=60)

                                                                              CGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGT             >  SRR4302544.320440/1‑94 (MQ=60)

                                                                                CCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAC           <  SRR4302544.3454030/94‑1 (MQ=60)

                                                                                CCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAC           >  SRR4302544.3827779/1‑94 (MQ=60)

                                                                                CCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAC           <  SRR4302544.1670775/94‑1 (MQ=60)

                                                                                     CATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCC      <  SRR4302544.1327195/94‑1 (MQ=60)

                                                                                     CATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCC      >  SRR4302544.3009350/1‑94 (MQ=60)

                                                                                      ATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCA     >  SRR4302544.1789548/1‑94 (MQ=60)

                                                                                      ATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCA     <  SRR4302544.2270304/94‑1 (MQ=60)

                                                                                        CAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGT   >  SRR4302544.2728480/1‑94 (MQ=60)

                                                                                         AGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTT  >  SRR4302544.2713404/1‑94 (MQ=60)

CCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTT  >  NC_000913/2111497‑2111679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`