Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,162,354 A→G E19E (GAA→GAG)  baeR → BaeR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,162,3540AG97.7% 411.1 / ‑1.1 131E19E (GAA→GAG) baeRBaeR
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (59/69);  total (60/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

Only 100 of 134 total aligned reads displayed.

AGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAACCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTA  >  NC_000913/2162264‑2162443
                                                                                          |                                                                                         
agTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGcc                                                                                         <  2:2804945/93‑1 (MQ=255)
agTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGcc                                                                                         <  1:1621180/93‑1 (MQ=255)
   tACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGaa                                                                                      >  2:4037326/1‑93 (MQ=255)
   tACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGaa                                                                                      <  2:3837539/93‑1 (MQ=255)
   tACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGaa                                                                                      <  2:1162342/93‑1 (MQ=255)
   tACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGaa                                                                                      <  1:2784881/93‑1 (MQ=255)
    aCCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAg                                                                                     >  2:547291/1‑93 (MQ=255)
     ccGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGc                                                                                    <  2:2789272/93‑1 (MQ=255)
     ccGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGc                                                                                    <  2:152913/93‑1 (MQ=255)
     ccGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGc                                                                                    <  1:557087/93‑1 (MQ=255)
      cGCTGGAGCGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCt                                                                                   <  1:1387462/93‑1 (MQ=255)
       gCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTg                                                                                  >  2:970201/1‑93 (MQ=255)
         tGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTggg                                                                                <  1:3916486/93‑1 (MQ=255)
         tGGAACGGGATTTACAGAGAGAAGTAAGACCGAGTTACCAATAGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGCGCCGACGCTggg                                                                                <  2:4200326/93‑1 (MQ=255)
          ggAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTgggg                                                                               <  1:3300655/93‑1 (MQ=255)
           gAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGc                                                                              >  2:4487170/1‑93 (MQ=255)
            aaCGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCa                                                                             <  1:2833999/93‑1 (MQ=255)
             aCGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAg                                                                            >  2:1302541/1‑93 (MQ=255)
              cGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGt                                                                           <  2:1700994/93‑1 (MQ=255)
              agggATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACCCACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGt                                                                           <  1:1266250/92‑1 (MQ=255)
               gggATTTTAAGAGAGAAGTAAGACCGAATTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGtt                                                                          <  2:3199504/93‑1 (MQ=255)
               gggATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGtt                                                                          >  2:2469263/1‑93 (MQ=255)
               gggATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGtt                                                                          >  2:2728658/1‑93 (MQ=255)
               gggATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGtt                                                                          <  1:272781/93‑1 (MQ=255)
               gggATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGCATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGtg                                                                          >  1:2689879/1‑92 (MQ=255)
                ggATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTg                                                                         >  2:351508/1‑93 (MQ=255)
                ggATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTg                                                                         >  1:2249895/1‑93 (MQ=255)
                 gATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGCAGATGGGGGAGTTGc                                                                        >  2:2694221/1‑93 (MQ=255)
                    ttACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCa                                                                     <  1:100157/93‑1 (MQ=255)
                    ttACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCa                                                                     >  2:3223293/1‑93 (MQ=255)
                     tACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCAt                                                                    <  2:408128/93‑1 (MQ=255)
                      aCAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCAtt                                                                   <  2:929010/93‑1 (MQ=255)
                        agagagAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGa                                                                 <  2:2434745/93‑1 (MQ=255)
                        agagagAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGa                                                                 >  1:4593738/1‑93 (MQ=255)
                         gagagaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGAt                                                                <  1:1102587/93‑1 (MQ=255)
                          agagaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGAtt                                                               <  1:427779/93‑1 (MQ=255)
                          agagaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGCGCAGTTGCTCATTGAtt                                                               >  2:4536600/1‑93 (MQ=255)
                           gagaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTa                                                              >  1:1366456/1‑93 (MQ=255)
                             gaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATc                                                            <  1:1408749/93‑1 (MQ=255)
                             gaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATc                                                            <  1:4332885/93‑1 (MQ=255)
                                gTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGc                                                         <  1:1855587/93‑1 (MQ=255)
                                   tGACCGAGTTACCAATCGACGAACACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCTTTGATTATCTGCGtg                                                      >  1:1422672/1‑93 (MQ=255)
                                   tGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCggg                                                      >  1:795243/1‑91 (MQ=255)
                                   tGACCGAGTCACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGtg                                                      <  2:3259424/93‑1 (MQ=255)
                                       cGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGtgctgc                                                  >  2:3351300/1‑93 (MQ=255)
                                        gAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCg                                                 >  1:738142/1‑93 (MQ=255)
                                          gTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAg                                               >  1:3018363/1‑93 (MQ=255)
                                           ttACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGc                                              <  1:2446873/93‑1 (MQ=255)
                                             aCCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTa                                            <  2:898058/93‑1 (MQ=255)
                                              ccAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTAt                                           >  1:4441312/1‑93 (MQ=255)
                                              ccAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTAt                                           >  1:1716471/1‑93 (MQ=255)
                                               cAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATg                                          <  1:1875911/93‑1 (MQ=255)
                                               cAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATg                                          >  1:2117833/1‑93 (MQ=255)
                                                aaTCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATgc                                         <  1:437451/93‑1 (MQ=255)
                                                aaTCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATgc                                         >  2:4504985/1‑93 (MQ=255)
                                                aaTCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATgc                                         <  1:4512787/93‑1 (MQ=255)
                                                aaTCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAACCGAAGCTGGGGCAGTTGCTCATGGATTATCCGCGTGCTGCGAGCCAtac                                         >  2:2861892/1‑91 (MQ=255)
                                                  tCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATgcgc                                       >  1:511573/1‑93 (MQ=255)
                                                  tCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAACCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATgcgc                                       <  2:4045837/93‑1 (MQ=255)
                                                    gacgaAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCg                                     >  1:2522982/1‑93 (MQ=255)
                                                        aaaaCACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGc                                 >  2:1438321/1‑93 (MQ=255)
                                                         aaaCACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCt                                >  1:263930/1‑93 (MQ=255)
                                                          aaCACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCtt                               <  2:514001/93‑1 (MQ=255)
                                                          aaCACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCtt                               >  2:535883/1‑93 (MQ=255)
                                                          aaCACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCtt                               <  1:23184/93‑1 (MQ=255)
                                                            cacacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTAt                             <  2:1817848/93‑1 (MQ=255)
                                                            cacacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTAt                             <  1:4678246/93‑1 (MQ=255)
                                                             acacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATc                            <  1:745931/93‑1 (MQ=255)
                                                             acacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATc                            >  2:2346391/1‑93 (MQ=255)
                                                              cacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCa                           <  1:233814/93‑1 (MQ=255)
                                                              cacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCa                           <  2:4655689/93‑1 (MQ=255)
                                                              cacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCa                           <  2:4655690/93‑1 (MQ=255)
                                                               cccGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAg                          <  2:4534078/92‑1 (MQ=255)
                                                                 cgcgTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGcc                        >  2:2732869/1‑93 (MQ=255)
                                                                  gcgTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCa                       >  2:2323536/1‑93 (MQ=255)
                                                                  gcgTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCa                       <  2:4555083/93‑1 (MQ=255)
                                                                   cgTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGcccc                      >  2:3414048/1‑91 (MQ=255)
                                                                   cgTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCAc                      <  1:3191609/93‑1 (MQ=255)
                                                                   cgTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCAc                      <  1:2471199/93‑1 (MQ=255)
                                                                    gTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACg                     >  2:2978838/1‑93 (MQ=255)
                                                                    gTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACg                     >  1:2454850/1‑93 (MQ=255)
                                                                     tATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACgg                    <  1:4173314/93‑1 (MQ=255)
                                                                     tATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACgg                    <  1:3364763/93‑1 (MQ=255)
                                                                     tATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACgg                    >  1:2454954/1‑93 (MQ=255)
                                                                      aTTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGc                   <  1:790001/93‑1 (MQ=255)
                                                                         ttGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGAt                >  2:3482325/1‑93 (MQ=255)
                                                                          tGATGGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATc               <  1:1044732/93‑1 (MQ=255)
                                                                               gTGGAAGATGAACCGAAGCTGGCGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTa          >  1:4035052/1‑93 (MQ=255)
                                                                                tGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTAc         >  2:887624/1‑93 (MQ=255)
                                                                                 ggAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACt        <  1:3721854/93‑1 (MQ=255)
                                                                                 ggAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACt        >  1:3664783/1‑93 (MQ=255)
                                                                                  gAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTg       >  2:2530119/1‑93 (MQ=255)
                                                                                  gAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTg       <  2:652501/93‑1 (MQ=255)
                                                                                  gAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCACCCACGGCGATCAGGTACTg       >  2:3542744/1‑93 (MQ=255)
                                                                                    aGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGcc     >  1:2598201/1‑93 (MQ=255)
                                                                                     gATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCg    >  2:617867/1‑93 (MQ=255)
                                                                                     gATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCg    >  1:1553222/1‑93 (MQ=255)
                                                                                     gATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCg    >  1:1430529/1‑93 (MQ=255)
                                                                                      aTGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTAGTGCCGt   >  1:4052699/1‑93 (MQ=255)
                                                                                       tGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTa  <  2:1451277/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAACCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTA  >  NC_000913/2162264‑2162443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGAGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAACCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTATGTG  >  NC_000913/2162261‑2162447
                                                                                             |                                                                                             
TAGAGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAG                                                                                               <  SRR4302544.1934584/94‑1 (MQ=60)
 AGAGTTACCGCTGGAGCGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGC                                                                                              <  SRR4302544.2069881/94‑1 (MQ=60)
 AGAGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGC                                                                                              <  SRR4302544.4471767/94‑1 (MQ=60)
 AGAGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGC                                                                                              >  SRR4302544.3808232/1‑94 (MQ=60)
  GAGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCC                                                                                             <  SRR4302544.1990507/94‑1 (MQ=60)
   AGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCG                                                                                            <  SRR4302544.1637147/94‑1 (MQ=60)
      TACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAG                                                                                         <  SRR4302544.3539626/94‑1 (MQ=60)
      TACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAG                                                                                         <  SRR4302544.2811678/94‑1 (MQ=60)
        CCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCT                                                                                       <  SRR4302544.562532/94‑1 (MQ=60)
         CGCTGGAGCGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTG                                                                                      <  SRR4302544.1401318/94‑1 (MQ=60)
            TGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGG                                                                                   <  SRR4302544.3955385/94‑1 (MQ=60)
             GGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGC                                                                                  <  SRR4302544.3332094/94‑1 (MQ=60)
               AACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAG                                                                                <  SRR4302544.2861381/94‑1 (MQ=60)
                 CGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGCATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTG                                                                              >  SRR4302544.2715673/1‑94 (MQ=60)
                 AGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACCCACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTT                                                                              <  SRR4302544.1278704/94‑1 (MQ=60)
                  GGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTG                                                                             <  SRR4302544.275651/94‑1 (MQ=60)
                  GGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTG                                                                             >  SRR4302544.2271037/1‑94 (MQ=60)
                     ATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTC                                                                          >  SRR4302544.1854714/1‑94 (MQ=60)
                       TTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCAT                                                                        <  SRR4302544.101077/94‑1 (MQ=60)
                          CAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGA                                                                     >  SRR4302544.4639434/1‑94 (MQ=60)
                            GAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATT                                                                   <  SRR4302544.1113568/94‑1 (MQ=60)
                             AGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTA                                                                  >  SRR4302544.4047979/1‑94 (MQ=60)
                             AGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTA                                                                  >  SRR4302544.1380041/1‑94 (MQ=60)
                             AGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTA                                                                  <  SRR4302544.432127/94‑1 (MQ=60)
                                GAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCT                                                               <  SRR4302544.4376996/94‑1 (MQ=60)
                                GAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCT                                                               <  SRR4302544.1422775/94‑1 (MQ=60)
                                 AAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTG                                                              >  SRR4302544.3203280/1‑94 (MQ=60)
                                   GTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCG                                                            <  SRR4302544.1873533/94‑1 (MQ=60)
                                     ATGACCGAGTTACCAATCGACGAACACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCTTTGATTATCTGCGTG                                                          >  SRR4302544.1436829/1‑94 (MQ=60)
                                     ATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGGG                                                          >  SRR4302544.802944/1‑94 (MQ=60)
                                          CGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCG                                                     >  SRR4302544.745365/1‑94 (MQ=60)
                                            AGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAG                                                   >  SRR4302544.3047294/1‑94 (MQ=60)
                                              TTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCT                                                 <  SRR4302544.2470201/94‑1 (MQ=60)
                                               TACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTA                                                >  SRR4302544.1219064/1‑94 (MQ=60)
                                                ACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTAT                                               <  SRR4302544.468841/94‑1 (MQ=60)
                                                ACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTAT                                               >  SRR4302544.4486087/1‑94 (MQ=60)
                                                ACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTAT                                               >  SRR4302544.1733346/1‑94 (MQ=60)
                                                 CCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATG                                              >  SRR4302544.2138171/1‑94 (MQ=60)
                                                  CAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGC                                             <  SRR4302544.1894048/94‑1 (MQ=60)
                                                   AATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCG                                            <  SRR4302544.441849/94‑1 (MQ=60)
                                                   AATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCG                                            <  SRR4302544.4558004/94‑1 (MQ=60)
                                                   AATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCG                                            >  SRR4302544.2371197/1‑94 (MQ=60)
                                                    ATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGC                                           >  SRR4302544.516619/1‑94 (MQ=60)
                                                      CGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCG                                         >  SRR4302544.2547225/1‑94 (MQ=60)
                                                           AAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCT                                    >  SRR4302544.266719/1‑94 (MQ=60)
                                                            AAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTT                                   >  SRR4302544.68868/1‑94 (MQ=60)
                                                            AAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTT                                   >  SRR4302544.2490793/1‑94 (MQ=60)
                                                             AACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTA                                  <  SRR4302544.23410/94‑1 (MQ=60)
                                                              ACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTAT                                 <  SRR4302544.765703/94‑1 (MQ=60)
                                                               CACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATC                                <  SRR4302544.1473998/94‑1 (MQ=60)
                                                               CACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATC                                <  SRR4302544.4724496/94‑1 (MQ=60)
                                                                ACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCA                               <  SRR4302544.753245/94‑1 (MQ=60)
                                                                 CACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAG                              <  SRR4302544.236249/94‑1 (MQ=60)
                                                                   CCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCC                            >  SRR4302544.2144530/1‑94 (MQ=60)
                                                                      CGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACG                         >  SRR4302544.2478268/1‑94 (MQ=60)
                                                                      CGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACG                         <  SRR4302544.3222021/94‑1 (MQ=60)
                                                                      CGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACG                         <  SRR4302544.2494818/94‑1 (MQ=60)
                                                                      CGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACG                         <  SRR4302544.2649258/94‑1 (MQ=60)
                                                                       GTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGG                        >  SRR4302544.2372600/1‑94 (MQ=60)
                                                                       GTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGG                        >  SRR4302544.2478374/1‑94 (MQ=60)
                                                                        TATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTGTCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGC                       >  SRR4302544.4446301/1‑94 (MQ=60)
                                                                        TATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGC                       >  SRR4302544.4319980/1‑94 (MQ=60)
                                                                        TATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGC                       <  SRR4302544.4214647/94‑1 (MQ=60)
                                                                        TATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGC                       <  SRR4302544.3396881/94‑1 (MQ=60)
                                                                         ATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCG                      <  SRR4302544.797642/94‑1 (MQ=60)
                                                                             TGATGGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCA                  <  SRR4302544.1055094/94‑1 (MQ=60)
                                                                                TCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGT               <  SRR4302544.1410509/94‑1 (MQ=60)
                                                                                 CGTGGAAGATGAACCGAAGCTGGCGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTA              >  SRR4302544.4075242/1‑94 (MQ=60)
                                                                                   TGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGCTCAGGTACT            >  SRR4302544.2782684/1‑94 (MQ=60)
                                                                                   TGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACT            >  SRR4302544.3701329/1‑94 (MQ=60)
                                                                                    GGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTG           <  SRR4302544.3758870/94‑1 (MQ=60)
                                                                                      AAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCC         >  SRR4302544.2623178/1‑94 (MQ=60)
                                                                                       AGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCG        >  SRR4302544.1568680/1‑94 (MQ=60)
                                                                                       AGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCG        >  SRR4302544.1444758/1‑94 (MQ=60)
                                                                                        GATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTAGTGCCGT       >  SRR4302544.4093058/1‑94 (MQ=60)
                                                                                           GAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTATG    >  SRR4302544.4736915/1‑94 (MQ=60)
                                                                                           GAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTATG    <  SRR4302544.1211352/94‑1 (MQ=60)
                                                                                            AGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCCCGGCGATCAGGTACTGCCGTATGT   >  SRR4302544.814990/1‑94 (MQ=60)
                                                                                            AGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTATGT   >  SRR4302544.814991/1‑94 (MQ=60)
                                                                                             GCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTATGTG  <  SRR4302544.2194102/94‑1 (MQ=60)
                                                                                             |                                                                                             
TAGAGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAACCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTATGTG  >  NC_000913/2162261‑2162447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑