Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,162,354 A→G E19E (GAA→GAG)  baeR → BaeR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,162,3540AG100.0% 773.3 / NA 234E19E (GAA→GAG) baeRBaeR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (112/122);  total (112/122)

Only 100 of 238 total aligned reads displayed.

AGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAACCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTA  >  NC_000913/2162264‑2162443
                                                                                          |                                                                                         
   tACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTCTTTTGATCGTGGAAGATGAGCCGaa                                                                                      >  1:2237096/1‑93 (MQ=255)
   tACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGaa                                                                                      >  1:764891/1‑93 (MQ=255)
   tACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGaa                                                                                      <  2:4123155/93‑1 (MQ=255)
    aCCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTATCAATCGACGATAACACACTGCGTATTTTGATCGATGAAGATGAGCCGAAg                                                                                     >  1:6725782/1‑93 (MQ=255)
    aCCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAg                                                                                     >  2:1696913/1‑93 (MQ=255)
     ccGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGc                                                                                    <  2:2960520/93‑1 (MQ=255)
     ccGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGc                                                                                    <  1:6438534/93‑1 (MQ=255)
     ccGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGc                                                                                    <  2:2466956/93‑1 (MQ=255)
      cGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCt                                                                                   <  2:2629674/93‑1 (MQ=255)
         tGGAACGGGATTTACAGAGAGCAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTggg                                                                                >  2:2723470/1‑93 (MQ=255)
         aggtaCGTGATGTACAGAGAGAAGTATGACCGAGTTAGCAATCGACGAAAACACACCGCGTATCTTGATCGTGGAAGATGAGCCGAAGCTggg                                                                                <  1:102246/89‑1 (MQ=255)
          ggAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTgggg                                                                               <  2:5049185/93‑1 (MQ=255)
           gAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGc                                                                              <  1:3116815/93‑1 (MQ=255)
             aCGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAg                                                                            >  2:638746/1‑93 (MQ=255)
             aCGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAg                                                                            >  1:396539/1‑93 (MQ=255)
              cGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGt                                                                           <  2:1432607/93‑1 (MQ=255)
               gggATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGtt                                                                          <  1:2343674/93‑1 (MQ=255)
                  aTTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCt                                                                       >  1:207169/1‑93 (MQ=255)
                   tttACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTc                                                                      <  2:5559471/93‑1 (MQ=255)
                   tttACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTc                                                                      <  1:4337155/93‑1 (MQ=255)
                   tttACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTc                                                                      >  1:1213757/1‑93 (MQ=255)
                         gagagaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGAt                                                                <  2:5324421/93‑1 (MQ=255)
                         gagagaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGAt                                                                <  1:3335643/93‑1 (MQ=255)
                          agagaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGAtt                                                               <  1:6358045/93‑1 (MQ=255)
                           gagaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTa                                                              >  2:2376712/1‑93 (MQ=255)
                           gagaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTa                                                              >  2:1177174/1‑93 (MQ=255)
                            agaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTAt                                                             <  2:649635/93‑1 (MQ=255)
                             gaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATc                                                            >  2:3873893/1‑93 (MQ=255)
                             gaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATc                                                            <  2:5778167/93‑1 (MQ=255)
                             gaAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATc                                                            <  2:5801998/93‑1 (MQ=255)
                              aaGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCt                                                           >  2:3100613/1‑93 (MQ=255)
                              aaGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCt                                                           >  1:1284807/1‑93 (MQ=255)
                              aaGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCt                                                           <  2:6716896/93‑1 (MQ=255)
                               aGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTg                                                          <  1:3699281/93‑1 (MQ=255)
                               aGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTg                                                          <  1:5403408/93‑1 (MQ=255)
                               aGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTg                                                          >  1:6465106/1‑93 (MQ=255)
                                 tATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCg                                                        <  2:1716547/93‑1 (MQ=255)
                                    gACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGtgc                                                     >  1:940103/1‑93 (MQ=255)
                                    gACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGtgc                                                     <  2:87339/93‑1 (MQ=255)
                                     aCCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGtgct                                                    <  2:4412757/93‑1 (MQ=255)
                                     aCCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGtgct                                                    <  1:4414109/93‑1 (MQ=255)
                                     aCCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTAGCTCATTGATTATCTGCGtgct                                                    >  1:1632873/1‑93 (MQ=255)
                                      ccGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGtgctg                                                   >  2:5447652/1‑93 (MQ=255)
                                      ccGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGtgctg                                                   <  1:426751/93‑1 (MQ=255)
                                        gAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCg                                                 >  1:4127753/1‑93 (MQ=255)
                                          gTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAg                                               >  2:1958375/1‑93 (MQ=255)
                                          gTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAg                                               >  1:5604388/1‑93 (MQ=255)
                                            tACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCt                                             <  2:6174860/93‑1 (MQ=255)
                                             aCCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTa                                            <  2:2232908/93‑1 (MQ=255)
                                             aCCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTa                                            <  2:2668672/93‑1 (MQ=255)
                                              ccAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTAt                                           <  1:1919761/93‑1 (MQ=255)
                                                aaTCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATgc                                         >  1:3268445/1‑93 (MQ=255)
                                                aaTCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATgc                                         <  1:3869497/93‑1 (MQ=255)
                                                aaTCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATgc                                         >  2:6644755/1‑93 (MQ=255)
                                                 aTCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATgcg                                        >  1:4853150/1‑93 (MQ=255)
                                                      cgaAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGAc                                   <  1:114626/93‑1 (MQ=255)
                                                       gaAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACg                                  <  2:1321344/93‑1 (MQ=255)
                                                        aaaaCACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGc                                 >  1:5854268/1‑93 (MQ=255)
                                                        aaaaCACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGc                                 <  1:5322940/93‑1 (MQ=255)
                                                         aaaCACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCt                                <  2:4944677/93‑1 (MQ=255)
                                                           acacacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTa                              >  2:3417062/1‑93 (MQ=255)
                                                             acacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTtctc                            >  2:1620085/1‑93 (MQ=255)
                                                             acacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATc                            >  2:4182744/1‑93 (MQ=255)
                                                             acacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATc                            <  1:5054673/93‑1 (MQ=255)
                                                             acacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATc                            <  2:3566378/93‑1 (MQ=255)
                                                             acacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATc                            >  1:1623954/1‑93 (MQ=255)
                                                             acacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATc                            <  1:4616300/93‑1 (MQ=255)
                                                             acacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATc                            <  2:194335/93‑1 (MQ=255)
                                                             acacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCACTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATc                            >  2:2886619/1‑93 (MQ=255)
                                                              cacCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCa                           >  1:3632373/1‑93 (MQ=255)
                                                                 cgcgTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGcc                        <  1:5271723/93‑1 (MQ=255)
                                                                  gcgTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCa                       <  1:6306703/93‑1 (MQ=255)
                                                                   cgTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCAc                      <  1:2530101/93‑1 (MQ=255)
                                                                    gTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACg                     >  1:1543180/1‑93 (MQ=255)
                                                                     tATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACgg                    >  1:4490117/1‑93 (MQ=255)
                                                                      aTTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGc                   >  2:4161821/1‑93 (MQ=255)
                                                                      aTTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGc                   >  1:781245/1‑93 (MQ=255)
                                                                        tttGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGa                 <  2:525037/93‑1 (MQ=255)
                                                                         ttGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGAt                <  1:5713182/93‑1 (MQ=255)
                                                                         ttGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGAt                >  2:3167084/1‑93 (MQ=255)
                                                                          tGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATc               <  1:453567/93‑1 (MQ=255)
                                                                           gATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCa              <  2:807450/93‑1 (MQ=255)
                                                                           gATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCa              >  2:3050873/1‑93 (MQ=255)
                                                                            aTCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAg             >  1:1260123/1‑93 (MQ=255)
                                                                            aTCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAg             >  1:5462561/1‑93 (MQ=255)
                                                                             tCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAgg            <  2:700668/93‑1 (MQ=255)
                                                                             tCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAgg            <  2:6255217/93‑1 (MQ=255)
                                                                              cGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGt           <  1:5528265/93‑1 (MQ=255)
                                                                              cGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGt           <  2:6075615/93‑1 (MQ=255)
                                                                               gTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTa          >  2:4300300/1‑93 (MQ=255)
                                                                               gTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTa          <  2:5085554/93‑1 (MQ=255)
                                                                               gTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTa          >  1:2929581/1‑93 (MQ=255)
                                                                               gTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTa          <  2:4889242/93‑1 (MQ=255)
                                                                                tGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTAc         >  1:4660465/1‑93 (MQ=255)
                                                                                 ggAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACt        >  2:373881/1‑93 (MQ=255)
                                                                                     gATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCg    >  2:2792172/1‑93 (MQ=255)
                                                                                      aTGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGt   >  2:1013506/1‑93 (MQ=255)
                                                                                       tGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTa  >  2:5848056/1‑93 (MQ=255)
                                                                                       tGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTa  <  1:1748646/93‑1 (MQ=255)
                                                                                       tGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTa  >  1:2967869/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAACCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTA  >  NC_000913/2162264‑2162443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGAGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAACCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTATGTG  >  NC_000913/2162261‑2162447
                                                                                             |                                                                                             
TAGAGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAG                                                                                               <  SRR4302536.3893175/94‑1 (MQ=60)
 AGAGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGC                                                                                              <  SRR4302536.4539091/94‑1 (MQ=60)
 AGAGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGC                                                                                              >  SRR4302536.4466300/1‑94 (MQ=60)
  GAGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCC                                                                                             <  SRR4302536.4397735/94‑1 (MQ=60)
  GAGTTACCGCTGGAACGGCATTTACAGAGAGAAGTATGACTGAGTTACCAATCGACGAAAACACCCCGCGTATTTTGATCGTGGAAGATGAGCC                                                                                             <  SRR4302536.3152042/94‑1 (MQ=60)
    GTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGGGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGA                                                                                           >  SRR4302536.4439623/1‑94 (MQ=60)
     TTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTCTTTTGATCGTGGAAGATGAGCCGAA                                                                                          >  SRR4302536.2304772/1‑94 (MQ=60)
     TTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAG                                                                                          >  SRR4302536.2376983/1‑94 (MQ=60)
     TTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAA                                                                                          >  SRR4302536.787319/1‑94 (MQ=60)
      TACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTATCAATCGACGATAACACACTGCGTATTTTGATCGATGAAGATGAGCCGAAG                                                                                         >  SRR4302536.6925570/1‑94 (MQ=60)
      TACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAG                                                                                         >  SRR4302536.3496294/1‑94 (MQ=60)
      TACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAG                                                                                         >  SRR4302536.5576925/1‑94 (MQ=60)
        CCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCT                                                                                       <  SRR4302536.6630932/94‑1 (MQ=60)
         CGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTG                                                                                      <  SRR4302536.477724/94‑1 (MQ=60)
          GCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGG                                                                                     <  SRR4302536.4019251/94‑1 (MQ=60)
           ctgtaaggaGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGG                                                                                    <  SRR4302536.2679222/85‑1 (MQ=60)
            aggtacgtgatgTACAGAGAGAAGTATGACCGAGTTAGCAATCGACGAAAACACACCGCGTATCTTGATCGTGGAAGATGAGCCGAAGCTGGGG                                                                                   <  SRR4302536.104983/82‑1 (MQ=60)
              GAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCA                                                                                 <  SRR4302536.3207866/94‑1 (MQ=60)
              GAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCA                                                                                 <  SRR4302536.4577043/94‑1 (MQ=60)
               AACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAG                                                                                >  SRR4302536.1238197/1‑94 (MQ=60)
               AACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAG                                                                                >  SRR4302536.407025/1‑94 (MQ=60)
               AACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAG                                                                                >  SRR4302536.214747/1‑94 (MQ=60)
                 CGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTT                                                                              <  SRR4302536.6022616/94‑1 (MQ=60)
                  GGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTG                                                                             <  SRR4302536.2414756/94‑1 (MQ=60)
                  GGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTG                                                                             <  SRR4302536.934448/94‑1 (MQ=60)
                  GGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTG                                                                             >  SRR4302536.4749929/1‑94 (MQ=60)
                   GGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGC                                                                            >  SRR4302536.6374003/1‑94 (MQ=60)
                    GATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCT                                                                           >  SRR4302536.212633/1‑94 (MQ=60)
                     ATTTACAGAGAGAGGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTC                                                                          <  SRR4302536.4598302/94‑1 (MQ=60)
                     ATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTC                                                                          >  SRR4302536.1250212/1‑94 (MQ=60)
                     ATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTC                                                                          >  SRR4302536.4233300/1‑94 (MQ=60)
                      TTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCA                                                                         <  SRR4302536.4467067/94‑1 (MQ=60)
                      TTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCA                                                                         <  SRR4302536.5216006/94‑1 (MQ=60)
                      TTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCA                                                                         <  SRR4302536.4662521/94‑1 (MQ=60)
                       TTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCAT                                                                        >  SRR4302536.4042385/1‑94 (MQ=60)
                        TACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATT                                                                       <  SRR4302536.941860/94‑1 (MQ=60)
                          CAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGA                                                                     <  SRR4302536.3487589/94‑1 (MQ=60)
                           AGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGAT                                                                    >  SRR4302536.73450/1‑94 (MQ=60)
                            GAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATT                                                                   <  SRR4302536.3434422/94‑1 (MQ=60)
                             AGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTA                                                                  <  SRR4302536.6547457/94‑1 (MQ=60)
                             AGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTA                                                                  >  SRR4302536.3897158/1‑94 (MQ=60)
                                GAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCT                                                               >  SRR4302536.1323255/1‑94 (MQ=60)
                                 AAGTATGACCGAGTTACCAATCGACGAAATCACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTG                                                              >  SRR4302536.4389327/1‑94 (MQ=60)
                                 AAGTATGACCGAGTTACCAATCGACGAAAACGCACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTG                                                              <  SRR4302536.4507398/94‑1 (MQ=60)
                                 AAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTG                                                              >  SRR4302536.6658081/1‑94 (MQ=60)
                                 AAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTG                                                              >  SRR4302536.1759849/1‑94 (MQ=60)
                                  AGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGC                                                             <  SRR4302536.3809903/94‑1 (MQ=60)
                                  AGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGC                                                             <  SRR4302536.5564817/94‑1 (MQ=60)
                                   GTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCG                                                            <  SRR4302536.468744/94‑1 (MQ=60)
                                      TGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGC                                                         >  SRR4302536.967838/1‑94 (MQ=60)
                                       GACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTAGCTCATTGATTATCTGCGTGCT                                                        >  SRR4302536.1680426/1‑94 (MQ=60)
                                        ACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTG                                                       <  SRR4302536.4546220/94‑1 (MQ=60)
                                        ACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTG                                                       >  SRR4302536.560618/1‑94 (MQ=60)
                                         cctgGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGC                                                      <  SRR4302536.3058939/90‑1 (MQ=60)
                                         CCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGC                                                      <  SRR4302536.437985/94‑1 (MQ=60)
                                         CCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGC                                                      <  SRR4302536.308153/94‑1 (MQ=60)
                                          CGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCG                                                     >  SRR4302536.4250115/1‑94 (MQ=60)
                                           GAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGA                                                    <  SRR4302536.2775622/94‑1 (MQ=60)
                                            AGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAG                                                   >  SRR4302536.5770925/1‑94 (MQ=60)
                                             GTTACCAATCGCCGAAAACACACCGCGTATTTTGATCGTGGCAGATGAGCCGAACCTGGGGCGGCTGCTCATTGATTATCTGCGTGCTGCGAGT                                                  >  SRR4302536.1124994/1‑94 (MQ=60)
                                              TTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCT                                                 <  SRR4302536.6578965/94‑1 (MQ=60)
                                              TTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCT                                                 >  SRR4302536.1322950/1‑94 (MQ=60)
                                               TACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTA                                                >  SRR4302536.2558162/1‑94 (MQ=60)
                                               TACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTA                                                >  SRR4302536.1253222/1‑94 (MQ=60)
                                                 CCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATG                                              >  SRR4302536.5007768/1‑94 (MQ=60)
                                                 CCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATG                                              >  SRR4302536.1669500/1‑94 (MQ=60)
                                                 CCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATG                                              <  SRR4302536.1977525/94‑1 (MQ=60)
                                                  CAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGC                                             >  SRR4302536.3364401/1‑94 (MQ=60)
                                                   AATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCG                                            >  SRR4302536.4999182/1‑94 (MQ=60)
                                                   AATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCG                                            <  SRR4302536.3984618/94‑1 (MQ=60)
                                                    ATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGC                                           <  SRR4302536.6641319/94‑1 (MQ=60)
                                                    ATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGC                                           >  SRR4302536.798318/1‑94 (MQ=60)
                                                      CGACGAAAACACACCGCGTATTTTGATCGCGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGTTTATTCCCGTGCTGCGCGCTATGCGCCG                                         >  SRR4302536.3081129/1‑94 (MQ=60)
                                                         CGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACG                                      >  SRR4302536.1268214/1‑94 (MQ=60)
                                                         CGAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACG                                      <  SRR4302536.117663/94‑1 (MQ=60)
                                                          GAAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGC                                     >  SRR4302536.6028694/1‑94 (MQ=60)
                                                           AAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCT                                    <  SRR4302536.3754581/94‑1 (MQ=60)
                                                           AAAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCT                                    <  SRR4302536.5481953/94‑1 (MQ=60)
                                                            AAACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTT                                   >  SRR4302536.1674321/1‑94 (MQ=60)
                                                             AACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGTCGCTTA                                  >  SRR4302536.1548637/1‑94 (MQ=60)
                                                             AACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTA                                  <  SRR4302536.3035652/94‑1 (MQ=60)
                                                             AACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTA                                  >  SRR4302536.498284/1‑94 (MQ=60)
                                                              ACACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTAT                                 >  SRR4302536.6549834/1‑94 (MQ=60)
                                                               CACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATC                                <  SRR4302536.5429299/94‑1 (MQ=60)
                                                               CACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATC                                >  SRR4302536.1671248/1‑94 (MQ=60)
                                                               CACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATC                                >  SRR4302536.1951634/1‑94 (MQ=60)
                                                                ACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCA                               <  SRR4302536.6508158/94‑1 (MQ=60)
                                                                ACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCA                               <  SRR4302536.5206099/94‑1 (MQ=60)
                                                                ACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCA                               >  SRR4302536.3741024/1‑94 (MQ=60)
                                                                ACACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCA                               <  SRR4302536.4754960/94‑1 (MQ=60)
                                                                 CACCGCGTATTTTGATCGTGGAAGATGAGCCGCAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAG                              >  SRR4302536.1645864/1‑94 (MQ=60)
                                                                 CACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAG                              <  SRR4302536.3316824/94‑1 (MQ=60)
                                                                  acagaGTATTGTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGC                             <  SRR4302536.1473400/89‑1 (MQ=60)
                                                                  ACCGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGC                             >  SRR4302536.169771/1‑94 (MQ=60)
                                                                    CGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCA                           >  SRR4302536.2899834/1‑94 (MQ=60)
                                                                    CGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCA                           <  SRR4302536.5428843/94‑1 (MQ=60)
                                                                    CGCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCA                           >  SRR4302536.5637958/1‑94 (MQ=60)
                                                                     GCGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCAC                          <  SRR4302536.6494541/94‑1 (MQ=60)
                                                                      CGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACG                         <  SRR4302536.5904268/94‑1 (MQ=60)
                                                                      CGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACG                         >  SRR4302536.1588532/1‑94 (MQ=60)
                                                                      CGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACG                         >  SRR4302536.5766984/1‑94 (MQ=60)
                                                                      CGTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACG                         <  SRR4302536.2606357/94‑1 (MQ=60)
                                                                       GTATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGG                        >  SRR4302536.4624934/1‑94 (MQ=60)
                                                                        TATTTTGATCGTGTAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGC                       <  SRR4302536.4277422/94‑1 (MQ=60)
                                                                        TATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGC                       <  SRR4302536.6898058/94‑1 (MQ=60)
                                                                        TATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGC                       >  SRR4302536.804128/1‑94 (MQ=60)
                                                                        TATTTGGATCGTGGAAGAGGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGC                       <  SRR4302536.1700336/94‑1 (MQ=60)
                                                                         ATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGGTCATTGAGTATCTtcacacagcaagctataggacgaaacttatcagctagtgct                      >  SRR4302536.5560347/1‑53 (MQ=52)
                                                                         ATTTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCG                      >  SRR4302536.5857732/1‑94 (MQ=60)
                                                                           TTTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGAT                    <  SRR4302536.6896918/94‑1 (MQ=60)
                                                                            TTGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATC                   <  SRR4302536.5883093/94‑1 (MQ=60)
                                                                             TGATCGTGGAAGATGAGCCGAAGCTGGGTCAGGTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCA                  >  SRR4302536.1990430/1‑94 (MQ=60)
                                                                             TGATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCA                  <  SRR4302536.465471/94‑1 (MQ=60)
                                                                              TATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAG                 <  SRR4302536.2188194/94‑1 (MQ=60)
                                                                              GATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAG                 >  SRR4302536.1297919/1‑94 (MQ=60)
                                                                              GATCGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAG                 >  SRR4302536.5625843/1‑94 (MQ=60)
                                                                                 CGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTA              >  SRR4302536.3016029/1‑94 (MQ=60)
                                                                                 CGTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTA              <  SRR4302536.5693081/94‑1 (MQ=60)
                                                                                  GTGGAAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTAC             >  SRR4302536.4800595/1‑94 (MQ=60)
                                                                                      AAGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCC         >  SRR4302536.3367205/1‑94 (MQ=60)
                                                                                       AGATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCG        >  SRR4302536.4960502/1‑94 (MQ=60)
                                                                                        GATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGT       >  SRR4302536.1085033/1‑94 (MQ=60)
                                                                                        GATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGT       <  SRR4302536.5521053/94‑1 (MQ=60)
                                                                                        GATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGT       <  SRR4302536.5521052/94‑1 (MQ=60)
                                                                                         ATGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTA      >  SRR4302536.3055289/1‑94 (MQ=60)
                                                                                          TGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTAT     >  SRR4302536.1444179/1‑94 (MQ=60)
                                                                                          TGAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTAT     <  SRR4302536.1799755/94‑1 (MQ=60)
                                                                                           GAGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTATG    <  SRR4302536.3096836/94‑1 (MQ=60)
                                                                                            AGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTATGT   <  SRR4302536.2297842/94‑1 (MQ=60)
                                                                                            AGCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTATGT   >  SRR4302536.2544181/1‑94 (MQ=60)
                                                                                             GCCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTATGTG  <  SRR4302536.2702447/94‑1 (MQ=60)
                                                                                             |                                                                                             
TAGAGTTACCGCTGGAACGGGATTTACAGAGAGAAGTATGACCGAGTTACCAATCGACGAAAACACACCGCGTATTTTGATCGTGGAAGATGAACCGAAGCTGGGGCAGTTGCTCATTGATTATCTGCGTGCTGCGAGCTATGCGCCGACGCTTATCAGCCACGGCGATCAGGTACTGCCGTATGTG  >  NC_000913/2162261‑2162447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑