Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,165,874 T→C L50L (TTA→TTG)  b2084 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,165,8740TC98.8% 551.6 / ‑2.8 170L50L (TTA→TTG) b2084hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (101/67);  total (103/67)
Fisher's exact test for biased strand distribution p-value = 5.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 171 total aligned reads displayed.

TCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGC  >  NC_000913/2165784‑2165963
                                                                                          |                                                                                         
 ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAAc                                                                                        <  2:3598046/93‑1 (MQ=255)
  cGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAAcc                                                                                       <  2:3323754/93‑1 (MQ=255)
    tgtAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGt                                                                                     <  2:3933782/93‑1 (MQ=255)
     gtAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTc                                                                                    <  2:633845/93‑1 (MQ=255)
      tAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCt                                                                                   >  1:2182987/1‑93 (MQ=255)
        aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTc                                                                                 >  2:2014441/1‑93 (MQ=255)
        aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTc                                                                                 >  2:3015079/1‑93 (MQ=255)
        aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTc                                                                                 <  2:3826943/93‑1 (MQ=255)
         atatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTcc                                                                                <  1:1751746/93‑1 (MQ=255)
           atCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCAt                                                                              >  2:1752481/1‑93 (MQ=255)
           atCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCAt                                                                              <  2:1643146/93‑1 (MQ=255)
            tCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCAtt                                                                             <  1:4440112/93‑1 (MQ=255)
                  cGACCGGTAGCCATGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACcac                                                                       <  2:1585101/93‑1 (MQ=255)
                  cGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACcac                                                                       >  1:491454/1‑93 (MQ=255)
                   gACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACcaca                                                                      <  1:164382/93‑1 (MQ=255)
                   gACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACcaca                                                                      >  1:614142/1‑93 (MQ=255)
                    aCCGTTAGCTAGGCTGATGGATAACTCTGCTACACGGGCTTATTTTTTTTGGCATAAGCTCTATCTCTGGCAATCGTCTTTCATTGACTACAt                                                                     >  2:4194672/1‑93 (MQ=255)
                    aCCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACAt                                                                     <  2:2179847/93‑1 (MQ=255)
                    aCCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACAt                                                                     >  1:3530619/1‑93 (MQ=255)
                     ccGGTAGCCAGGCGGATGTAGAACTCTGCTACACGTGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCATCCGTCTTCCATTGACCACATc                                                                    <  2:607539/93‑1 (MQ=255)
                      cGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCg                                                                   >  1:4160209/1‑93 (MQ=255)
                       ggTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGa                                                                  >  2:1035425/1‑93 (MQ=255)
                       ggTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGa                                                                  <  1:805407/93‑1 (MQ=255)
                        gTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGAt                                                                 >  2:3514712/1‑93 (MQ=255)
                        gTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGAt                                                                 <  2:4321207/93‑1 (MQ=255)
                         tAGCCAGGCTGACGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATa                                                                >  1:4280055/1‑93 (MQ=255)
                          aGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAg                                                               <  2:4185089/93‑1 (MQ=255)
                           gCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGa                                                              >  1:829278/1‑93 (MQ=255)
                           gCCAGGCTGATGGAGAACTCTGCGACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGa                                                              <  2:3802968/93‑1 (MQ=255)
                             cAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAt                                                            >  2:1926590/1‑93 (MQ=255)
                             cAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAt                                                            >  1:1044122/1‑93 (MQ=255)
                             cAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAt                                                            >  1:1541710/1‑93 (MQ=255)
                             cAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAt                                                            >  2:3090845/1‑93 (MQ=255)
                              aGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtc                                                           >  1:3995661/1‑93 (MQ=255)
                                gCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtcct                                                         <  2:2846884/93‑1 (MQ=255)
                                 cTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtcctc                                                        <  2:234322/93‑1 (MQ=255)
                                     tGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTc                                                    <  1:3388641/93‑1 (MQ=255)
                                     tGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTc                                                    <  1:3343846/93‑1 (MQ=255)
                                       gagaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCAt                                                  >  1:3215129/1‑93 (MQ=255)
                                        agaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATa                                                 <  1:265651/93‑1 (MQ=255)
                                        agaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATa                                                 <  2:4488788/93‑1 (MQ=255)
                                        agaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCACTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATa                                                 >  2:3738829/1‑93 (MQ=255)
                                         gaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCTATATTATTCTCCTTCacat                                                >  1:3097730/1‑90 (MQ=255)
                                         gaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAg                                                <  2:2659542/93‑1 (MQ=255)
                                         gaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAg                                                >  2:1063412/1‑93 (MQ=255)
                                           aCTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCa                                              >  1:1284923/1‑93 (MQ=255)
                                             tctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACg                                            >  2:4526538/1‑93 (MQ=255)
                                             tctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACg                                            >  1:3941497/1‑93 (MQ=255)
                                              ctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGa                                           <  2:90719/93‑1 (MQ=255)
                                              ctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGa                                           <  1:785153/93‑1 (MQ=255)
                                                gCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATg                                         >  1:1091946/1‑93 (MQ=255)
                                                 cTACCCCGGCTTATTGGTTTAGGCATAAGCCCTAGCTCTGGCAACCGTCTGCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGc                                        <  1:4177710/93‑1 (MQ=255)
                                                  tACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGcc                                       >  1:1215752/1‑93 (MQ=255)
                                                  tACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGcc                                       <  1:1757995/93‑1 (MQ=255)
                                                  tACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGcc                                       >  1:2299882/1‑93 (MQ=255)
                                                   acacGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCt                                      <  2:2428746/93‑1 (MQ=255)
                                                      cGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTc                                   <  2:1280284/93‑1 (MQ=255)
                                                       gggCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCa                                  >  1:2955669/1‑93 (MQ=255)
                                                       gggCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCa                                  >  1:3705364/1‑93 (MQ=255)
                                                       gggCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCATCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGACTTTCa                                  >  1:991781/1‑93 (MQ=255)
                                                        ggCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGCTAGAATCCTCCTTCATAGCACGATGCCTTTCAc                                 >  1:4590607/1‑93 (MQ=255)
                                                        ggCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCAc                                 <  2:823407/93‑1 (MQ=255)
                                                          cTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACtt                               >  1:4515184/1‑93 (MQ=255)
                                                           ttATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTa                              >  2:336868/1‑93 (MQ=255)
                                                           ttATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTa                              <  2:588699/93‑1 (MQ=255)
                                                            tATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTAt                             <  2:442023/93‑1 (MQ=255)
                                                            tATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTAt                             >  1:3828897/1‑93 (MQ=255)
                                                            tATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTAt                             >  1:1728886/1‑93 (MQ=255)
                                                             aTTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATc                            >  2:4451296/1‑93 (MQ=255)
                                                             aTTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATc                            <  1:2177938/93‑1 (MQ=255)
                                                              tttttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCg                           <  2:2264475/93‑1 (MQ=255)
                                                              tttttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCg                           <  1:483032/93‑1 (MQ=255)
                                                                tttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGc                         >  1:1885810/1‑93 (MQ=255)
                                                                 ttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCa                        >  1:3315782/1‑93 (MQ=255)
                                                                  tttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCAt                       <  1:2289577/93‑1 (MQ=255)
                                                                  tttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCAt                       >  1:734590/1‑93 (MQ=255)
                                                                     aTGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGt                    >  2:1288792/1‑93 (MQ=255)
                                                                         aTAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTc                >  1:2654358/1‑93 (MQ=255)
                                                                          tAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTcc               >  1:408573/1‑93 (MQ=255)
                                                                          tAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTcc               <  2:3677135/93‑1 (MQ=255)
                                                                           aaGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTccc              >  1:380038/1‑93 (MQ=255)
                                                                               ccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAg          >  1:71594/1‑93 (MQ=255)
                                                                               ccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAg          >  2:3998256/1‑93 (MQ=255)
                                                                                cTATCTCTGGCAACCGTGTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAgg         >  1:2362425/1‑93 (MQ=255)
                                                                                cTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAgg         >  2:1864999/1‑93 (MQ=255)
                                                                                cTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAgg         >  2:4235421/1‑93 (MQ=255)
                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt        >  1:1650361/1‑93 (MQ=255)
                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt        >  2:1372687/1‑93 (MQ=255)
                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt        >  1:3501977/1‑93 (MQ=255)
                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt        >  1:2394578/1‑93 (MQ=255)
                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt        >  1:2497455/1‑93 (MQ=255)
                                                                                  aTCTCTGGCGACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTTCTACCACAGGtt       >  1:4611791/1‑93 (MQ=255)
                                                                                  aTCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCAGCGCGCTCCCACAGGtt       >  1:4196077/1‑93 (MQ=255)
                                                                                    ctctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTcc     <  1:1693443/93‑1 (MQ=255)
                                                                                    ctctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTcc     <  2:4358645/93‑1 (MQ=255)
                                                                                     tctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCg    >  1:1337214/1‑93 (MQ=255)
                                                                                     tctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCg    >  2:1574794/1‑93 (MQ=255)
                                                                                     tctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCg    >  2:2938298/1‑93 (MQ=255)
                                                                                     tctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCg    <  2:1861790/93‑1 (MQ=255)
                                                                                       tGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGc  <  2:3451788/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGC  >  NC_000913/2165784‑2165963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  >  NC_000913/2165781‑2165967
                                                                                             |                                                                                             
GTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGC                                                                                               >  SRR4302544.2195388/1‑94 (MQ=60)
GTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGC                                                                                               <  SRR4302544.1123223/94‑1 (MQ=60)
GTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGC                                                                                               >  SRR4302544.2195389/1‑94 (MQ=60)
 TTTCCGTGTAAATATCTGCCCGGCCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCA                                                                                              <  SRR4302544.657130/94‑1 (MQ=60)
  TTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAA                                                                                             >  SRR4302544.4547238/1‑94 (MQ=60)
   TCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAAC                                                                                            >  SRR4302544.3923664/1‑94 (MQ=60)
        GTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCT                                                                                       >  SRR4302544.2203648/1‑94 (MQ=60)
            ATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCA                                                                                   <  SRR4302544.1768893/94‑1 (MQ=60)
               TCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTG                                                                                <  SRR4302544.173260/94‑1 (MQ=60)
               TCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTG                                                                                <  SRR4302544.4484880/94‑1 (MQ=60)
               TCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTG                                                                                <  SRR4302544.1786578/94‑1 (MQ=60)
                   CCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCA                                                                            >  SRR4302544.204555/1‑94 (MQ=60)
                    CCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCAC                                                                           >  SRR4302544.496281/1‑94 (MQ=60)
                     CGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACA                                                                          >  SRR4302544.620164/1‑94 (MQ=60)
                     CGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACA                                                                          >  SRR4302544.644543/1‑94 (MQ=60)
                      GACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACAT                                                                         >  SRR4302544.3564385/1‑94 (MQ=60)
                      GACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACAT                                                                         <  SRR4302544.165993/94‑1 (MQ=60)
                        CCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCG                                                                       >  SRR4302544.4201418/1‑94 (MQ=60)
                          GGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGAT                                                                     <  SRR4302544.813258/94‑1 (MQ=60)
                          GGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGAT                                                                     <  SRR4302544.813260/94‑1 (MQ=60)
                           GTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATA                                                                    >  SRR4302544.1672460/1‑94 (MQ=60)
                           GTAGCCAGGCTGACGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATA                                                                    >  SRR4302544.4323763/1‑94 (MQ=60)
                            TAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAG                                                                   <  SRR4302544.1264383/94‑1 (MQ=60)
                             AGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGA                                                                  >  SRR4302544.837422/1‑94 (MQ=60)
                               CCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAT                                                                >  SRR4302544.1054481/1‑94 (MQ=60)
                               CCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAT                                                                >  SRR4302544.1557041/1‑94 (MQ=60)
                               CCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAT                                                                >  SRR4302544.1557042/1‑94 (MQ=60)
                                CAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATC                                                               >  SRR4302544.4035434/1‑94 (MQ=60)
                                 AGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCC                                                              <  SRR4302544.3349304/94‑1 (MQ=60)
                                 AGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCC                                                              >  SRR4302544.1749243/1‑94 (MQ=60)
                                    CTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCC                                                           >  SRR4302544.3400841/1‑94 (MQ=60)
                                    CTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCC                                                           >  SRR4302544.1185577/1‑94 (MQ=60)
                                      GATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTT                                                         >  SRR4302544.233715/1‑94 (MQ=60)
                                        TGGAGAATTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCA                                                       >  SRR4302544.4274353/1‑94 (MQ=60)
                                        TGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCA                                                       <  SRR4302544.3421024/94‑1 (MQ=60)
                                        TGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCA                                                       <  SRR4302544.3375734/94‑1 (MQ=60)
                                         GGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCAT                                                      >  SRR4302544.245370/1‑94 (MQ=60)
                                         GGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCAT                                                      >  SRR4302544.3245799/1‑94 (MQ=60)
                                          GAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATA                                                     >  SRR4302544.4420589/1‑94 (MQ=60)
                                           AGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCtatattattctccttcacat                                                    >  SRR4302544.3127229/1‑74 (MQ=60)
                                           AGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAG                                                    <  SRR4302544.268457/94‑1 (MQ=60)
                                            GAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGC                                                   >  SRR4302544.2309235/1‑94 (MQ=60)
                                             AACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCA                                                  >  SRR4302544.1297580/1‑94 (MQ=60)
                                               CTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACG                                                >  SRR4302544.3980655/1‑94 (MQ=60)
                                               CTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACG                                                >  SRR4302544.4707848/1‑94 (MQ=60)
                                                 CTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGAT                                              <  SRR4302544.792747/94‑1 (MQ=60)
                                                  TGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATG                                             >  SRR4302544.731149/1‑94 (MQ=60)
                                                  TGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATG                                             >  SRR4302544.1102810/1‑94 (MQ=60)
                                                    CTACCCCGGCTTATTGGTTTAGGCATAAGCCCTAGCTCTGGCAACCGTCTGCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCC                                           <  SRR4302544.4219095/94‑1 (MQ=60)
                                                    CTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCC                                           <  SRR4302544.4219093/94‑1 (MQ=60)
                                                    CTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCC                                           <  SRR4302544.3162947/94‑1 (MQ=60)
                                                    CTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCC                                           >  SRR4302544.1227785/1‑94 (MQ=60)
                                                    CTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCC                                           >  SRR4302544.2321594/1‑94 (MQ=60)
                                                    CTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCC                                           >  SRR4302544.2321593/1‑94 (MQ=60)
                                                     TACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCT                                          <  SRR4302544.1775199/94‑1 (MQ=60)
                                                         CGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCA                                      >  SRR4302544.2984052/1‑94 (MQ=60)
                                                         CGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCA                                      >  SRR4302544.3742237/1‑94 (MQ=60)
                                                         CGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCATCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGACTTTCA                                      >  SRR4302544.1001571/1‑94 (MQ=60)
                                                          GGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGCTAGAATCCTCCTTCATAGCACGATGCCTTTCAC                                     >  SRR4302544.4636285/1‑94 (MQ=60)
                                                            GCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTT                                   >  SRR4302544.4560408/1‑94 (MQ=60)
                                                              TTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTAT                                 >  SRR4302544.1745839/1‑94 (MQ=60)
                                                              TTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTAT                                 >  SRR4302544.82162/1‑94 (MQ=60)
                                                              TTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTAT                                 >  SRR4302544.4198148/1‑94 (MQ=60)
                                                              TTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTAT                                 >  SRR4302544.3866866/1‑94 (MQ=60)
                                                                ATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCG                               <  SRR4302544.2198567/94‑1 (MQ=60)
                                                                ATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCG                               <  SRR4302544.3756742/94‑1 (MQ=60)
                                                                 TTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGG                              <  SRR4302544.487763/94‑1 (MQ=60)
                                                                  TTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGC                             >  SRR4302544.1904035/1‑94 (MQ=60)
                                                                   TTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCA                            >  SRR4302544.3347362/1‑94 (MQ=60)
                                                                    TTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCAT                           >  SRR4302544.741771/1‑94 (MQ=60)
                                                                     TTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATC                          <  SRR4302544.2311159/94‑1 (MQ=60)
                                                                        ATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTG                       >  SRR4302544.3091635/1‑94 (MQ=60)
                                                                           CATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTC                    <  SRR4302544.2547043/94‑1 (MQ=60)
                                                                           CATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTC                    >  SRR4302544.2679812/1‑94 (MQ=60)
                                                                            ATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCC                   >  SRR4302544.412731/1‑94 (MQ=60)
                                                                             TAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCC                  >  SRR4302544.383903/1‑94 (MQ=60)
                                                                              AAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCGA                 >  SRR4302544.3719242/1‑94 (MQ=60)
                                                                              AAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCA                 <  SRR4302544.553416/94‑1 (MQ=60)
                                                                               AGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCAC                >  SRR4302544.3547342/1‑94 (MQ=60)
                                                                                 CCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAG              >  SRR4302544.72288/1‑94 (MQ=60)
                                                                                  CCTATCTCTGGCAACCGTGTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGG             >  SRR4302544.2384794/1‑94 (MQ=60)
                                                                                   CTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGT            >  SRR4302544.3994425/1‑94 (MQ=60)
                                                                                   CTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGT            >  SRR4302544.1666640/1‑94 (MQ=60)
                                                                                   CTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGT            >  SRR4302544.2521390/1‑94 (MQ=60)
                                                                                   CTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGT            >  SRR4302544.2417277/1‑94 (MQ=60)
                                                                                   CTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGT            >  SRR4302544.3535384/1‑94 (MQ=60)
                                                                                    TATCTCTGGCGACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTTCTACCACAGGTT           >  SRR4302544.4657587/1‑94 (MQ=60)
                                                                                    TATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTT           >  SRR4302544.1541278/1‑94 (MQ=60)
                                                                                    TATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTT           >  SRR4302544.4657586/1‑94 (MQ=60)
                                                                                    TATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCAGCGCGCTCCCACAGGTT           >  SRR4302544.4237758/1‑94 (MQ=60)
                                                                                       CTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGTACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCG        >  SRR4302544.1350405/1‑94 (MQ=60)
                                                                                       CTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCG        <  SRR4302544.1710153/94‑1 (MQ=60)
                                                                                         CTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGC      >  SRR4302544.2906817/1‑94 (MQ=60)
                                                                                             CAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  >  SRR4302544.559202/1‑94 (MQ=60)
                                                                                             CAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  >  SRR4302544.3172999/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  >  NC_000913/2165781‑2165967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑