Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,165,874 T→C L50L (TTA→TTG)  b2084 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,165,8740TC100.0% 810.7 / NA 249L50L (TTA→TTG) b2084hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (113/136);  total (113/136)

Only 100 of 250 total aligned reads displayed.

TCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGC  >  NC_000913/2165784‑2165963
                                                                                          |                                                                                         
tCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCaa                                                                                         <  2:3294336/93‑1 (MQ=255)
 ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAAc                                                                                        <  2:2467881/93‑1 (MQ=255)
   gtgtAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCg                                                                                      >  1:5133102/1‑93 (MQ=255)
     gtAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTc                                                                                    <  2:6396518/93‑1 (MQ=255)
     gtAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTc                                                                                    >  1:5530906/1‑93 (MQ=255)
      tAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCt                                                                                   <  2:2483279/93‑1 (MQ=255)
        aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTc                                                                                 >  2:5482454/1‑93 (MQ=255)
         atatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTcc                                                                                <  2:5839097/93‑1 (MQ=255)
          tatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCa                                                                               <  1:1691223/93‑1 (MQ=255)
          tatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCa                                                                               >  1:1772811/1‑93 (MQ=255)
           atCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCTTCTTCCAt                                                                              <  2:173539/93‑1 (MQ=255)
           atCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCAt                                                                              <  2:5647601/93‑1 (MQ=255)
            tCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCAtt                                                                             >  2:3108958/1‑93 (MQ=255)
             cTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTg                                                                            >  2:2862403/1‑93 (MQ=255)
               tcccGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGAc                                                                          <  1:2275824/92‑1 (MQ=255)
                  cGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACcac                                                                       >  2:166548/1‑93 (MQ=255)
                    aCCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACAt                                                                     >  2:3162008/1‑93 (MQ=255)
                     ccGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATc                                                                    <  1:4052898/93‑1 (MQ=255)
                     ccGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATc                                                                    <  2:2572660/93‑1 (MQ=255)
                     ccGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATc                                                                    <  2:2171984/93‑1 (MQ=255)
                       ggTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGa                                                                  >  1:3210182/1‑93 (MQ=255)
                       ggTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGa                                                                  >  1:2619167/1‑93 (MQ=255)
                        gTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGAt                                                                 >  1:3484413/1‑93 (MQ=255)
                         tAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCTTTGACCACATCGATa                                                                <  1:784814/93‑1 (MQ=255)
                            ccAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGaa                                                             <  1:3404512/93‑1 (MQ=255)
                             cAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAt                                                            <  2:1537600/93‑1 (MQ=255)
                             cAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAt                                                            <  1:4903902/93‑1 (MQ=255)
                              aGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtc                                                           <  1:3663627/93‑1 (MQ=255)
                               ggCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtcc                                                          >  1:5677342/1‑93 (MQ=255)
                               ggCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtcc                                                          <  1:4529563/93‑1 (MQ=255)
                                gCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtcct                                                         <  1:3264859/93‑1 (MQ=255)
                                 cTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtcctc                                                        <  2:4338213/93‑1 (MQ=255)
                                  tGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtcctcc                                                       >  2:2905017/1‑93 (MQ=255)
                                   gATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATcctcct                                                      <  1:2770091/93‑1 (MQ=255)
                                   gATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATcctcct                                                      <  2:4230793/93‑1 (MQ=255)
                                     tGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATACGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTc                                                    >  2:4819384/1‑93 (MQ=255)
                                     tGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTc                                                    <  2:1981554/93‑1 (MQ=255)
                                       gagaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCAt                                                  >  2:3557793/1‑93 (MQ=255)
                                        agaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATa                                                 >  2:2727887/1‑93 (MQ=255)
                                          aaCTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGc                                               <  2:3685884/93‑1 (MQ=255)
                                           aCTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCa                                              >  2:1461336/1‑93 (MQ=255)
                                           aCTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCa                                              <  1:594930/93‑1 (MQ=255)
                                            ctctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCAc                                             >  1:1854595/1‑93 (MQ=255)
                                              ctgctgGACGGGCTTATTTGTGTATGCATAAGGCCTATCTCTGGCAACCGTCTTCGATTGACCGCATCGATAGAATCCTCCTTCATAGCACgc                                           <  1:1922586/93‑2 (MQ=255)
                                              ctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGa                                           <  1:2578806/93‑1 (MQ=255)
                                               tGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGAt                                          <  1:2246659/93‑1 (MQ=255)
                                               tGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGAt                                          >  2:5522438/1‑93 (MQ=255)
                                                  tACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGcc                                       >  2:4373373/1‑93 (MQ=255)
                                                  tACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGcc                                       >  1:1208856/1‑93 (MQ=255)
                                                  tACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGcc                                       <  2:6690442/93‑1 (MQ=255)
                                                      cGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTc                                   >  1:4379576/1‑93 (MQ=255)
                                                      cGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTc                                   <  1:3134714/93‑1 (MQ=255)
                                                      cGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTc                                   <  1:278964/93‑1 (MQ=255)
                                                        ggCTTATTTTTTTATGCATAAGCGCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCAc                                 >  2:5935536/1‑93 (MQ=255)
                                                        ggCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCAc                                 >  1:4654480/1‑93 (MQ=255)
                                                        ggCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCAc                                 <  2:1035815/93‑1 (MQ=255)
                                                        ggCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCAc                                 <  1:3008935/93‑1 (MQ=255)
                                                          cTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACtt                               <  2:2390161/93‑1 (MQ=255)
                                                           ttATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTa                              >  2:463604/1‑93 (MQ=255)
                                                           ttATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTa                              <  2:6232182/93‑1 (MQ=255)
                                                            tATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTAt                             >  2:5418998/1‑93 (MQ=255)
                                                             aTTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATc                            >  2:3906302/1‑93 (MQ=255)
                                                             aTTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATc                            <  2:5014301/93‑1 (MQ=255)
                                                             aTTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATc                            <  2:2987533/93‑1 (MQ=255)
                                                              tttttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCg                           >  2:937897/1‑93 (MQ=255)
                                                              tttttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCg                           <  1:651546/93‑1 (MQ=255)
                                                              tttttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCg                           <  1:3480749/93‑1 (MQ=255)
                                                              tttttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTAACTTATCg                           <  1:6553428/93‑1 (MQ=255)
                                                               ttttttATGCATAAGCACTATCTCGGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCgg                          <  1:1529949/93‑1 (MQ=255)
                                                                tttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGc                         <  1:3596185/93‑1 (MQ=255)
                                                                 ttttATGCATAAGCCCTGTCTCTGGCAACCGTCTTCCATTGACCACATGGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCa                        <  1:4931092/93‑1 (MQ=255)
                                                                   ttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATc                      <  1:2301057/93‑1 (MQ=255)
                                                                    tATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCg                     <  1:1024422/93‑1 (MQ=255)
                                                                    tATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCg                     <  2:6650763/93‑1 (MQ=255)
                                                                     aTGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGt                    >  1:1371434/1‑93 (MQ=255)
                                                                       gCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTgt                  >  2:2062638/1‑92 (MQ=255)
                                                                       gCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGc                  <  1:2339941/93‑1 (MQ=255)
                                                                        cATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCt                 >  2:5275084/1‑93 (MQ=255)
                                                                        cATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCt                 <  2:4489719/93‑1 (MQ=255)
                                                                         aTAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTc                >  2:5622617/1‑93 (MQ=255)
                                                                         aTAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTc                >  1:2023822/1‑93 (MQ=255)
                                                                          tAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTcc               >  2:1334235/1‑93 (MQ=255)
                                                                          tAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTcc               <  1:3962275/93‑1 (MQ=255)
                                                                               ccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAg          >  1:6232589/1‑93 (MQ=255)
                                                                               ccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAg          >  2:4580263/1‑93 (MQ=255)
                                                                                cTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAgg         <  1:3894223/93‑1 (MQ=255)
                                                                                cTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAgg         <  1:5748808/93‑1 (MQ=255)
                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt        >  2:5135707/1‑93 (MQ=255)
                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt        <  1:5730516/93‑1 (MQ=255)
                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt        >  1:3501586/1‑93 (MQ=255)
                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt        >  1:3814334/1‑93 (MQ=255)
                                                                                  aTCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGtt       >  2:6490063/1‑93 (MQ=255)
                                                                                  aTCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGtt       >  1:6438995/1‑93 (MQ=255)
                                                                                   tctcTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTc      >  1:5371402/1‑93 (MQ=255)
                                                                                    ctctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTcc     <  2:604793/93‑1 (MQ=255)
                                                                                    ctctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTcc     <  1:6390393/93‑1 (MQ=255)
                                                                                     tctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCg    >  1:4112741/1‑93 (MQ=255)
                                                                                       tGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGc  <  1:2476661/93‑1 (MQ=255)
                                                                                       tGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGc  <  2:835093/93‑1 (MQ=255)
                                                                                       tGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGc  <  1:6642044/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGC  >  NC_000913/2165784‑2165963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  >  NC_000913/2165782‑2165967
                                                                                            |                                                                                             
TTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCA                                                                                              <  SRR4302536.3212506/94‑1 (MQ=60)
TTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCA                                                                                              <  SRR4302536.748527/94‑1 (MQ=60)
 TTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAA                                                                                             <  SRR4302536.3144145/94‑1 (MQ=60)
  TCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAAC                                                                                            >  SRR4302536.5887974/1‑94 (MQ=60)
    CGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCG                                                                                          >  SRR4302536.5286398/1‑94 (MQ=60)
      TGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTC                                                                                        >  SRR4302536.6521999/1‑94 (MQ=60)
      TGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTC                                                                                        >  SRR4302536.5695791/1‑94 (MQ=60)
        TAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTT                                                                                      <  SRR4302536.6614934/94‑1 (MQ=60)
        TAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTT                                                                                      >  SRR4302536.3584887/1‑94 (MQ=60)
        TAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTT                                                                                      <  SRR4302536.5131089/94‑1 (MQ=60)
         AAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGCACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTC                                                                                     >  SRR4302536.1463251/1‑94 (MQ=60)
           ATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCA                                                                                   >  SRR4302536.1826435/1‑94 (MQ=60)
            TATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCAT                                                                                  <  SRR4302536.1740194/94‑1 (MQ=60)
            TATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCAT                                                                                  <  SRR4302536.4588431/94‑1 (MQ=60)
             ATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATT                                                                                 <  SRR4302536.1237275/94‑1 (MQ=60)
                TGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGAC                                                                              <  SRR4302536.4327820/94‑1 (MQ=60)
                TGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGAC                                                                              <  SRR4302536.357320/94‑1 (MQ=60)
                 TCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACC                                                                             <  SRR4302536.2344794/94‑1 (MQ=60)
                    CGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACA                                                                          <  SRR4302536.3263521/94‑1 (MQ=60)
                      ACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATC                                                                        >  SRR4302536.5353450/1‑94 (MQ=60)
                      ACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATC                                                                        <  SRR4302536.790289/94‑1 (MQ=60)
                      ACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATC                                                                        <  SRR4302536.865655/94‑1 (MQ=60)
                       CCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCG                                                                       <  SRR4302536.4173050/94‑1 (MQ=60)
                       CCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCG                                                                       <  SRR4302536.6665346/94‑1 (MQ=60)
                        CGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGA                                                                      >  SRR4302536.2697730/1‑94 (MQ=60)
                        CGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGA                                                                      >  SRR4302536.3304344/1‑94 (MQ=60)
                         GGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGAT                                                                     >  SRR4302536.3588325/1‑94 (MQ=60)
                           TAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCTTTGACCACATCGATAG                                                                   <  SRR4302536.807793/94‑1 (MQ=60)
                           TAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAG                                                                   <  SRR4302536.5917388/94‑1 (MQ=60)
                             GCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAA                                                                 >  SRR4302536.2786037/1‑94 (MQ=60)
                              CCAGGCTGATGGAGAGCTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAT                                                                <  SRR4302536.4064241/94‑1 (MQ=60)
                              CCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAT                                                                <  SRR4302536.3505770/94‑1 (MQ=60)
                              CCAGGCTGATGGAGAACTCTGCTACACGAGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAT                                                                <  SRR4302536.2076787/94‑1 (MQ=60)
                               CAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATC                                                               >  SRR4302536.33062/1‑94 (MQ=60)
                               CAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATC                                                               <  SRR4302536.5051346/94‑1 (MQ=60)
                                AGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCC                                                              <  SRR4302536.302570/94‑1 (MQ=60)
                                AGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCC                                                              <  SRR4302536.3773158/94‑1 (MQ=60)
                                AGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCC                                                              >  SRR4302536.5846138/1‑94 (MQ=60)
                                 GGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCT                                                             >  SRR4302536.5939415/1‑94 (MQ=60)
                                 GGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCT                                                             <  SRR4302536.4665354/94‑1 (MQ=60)
                                  GCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTC                                                            <  SRR4302536.6317455/94‑1 (MQ=60)
                                  GCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTC                                                            <  SRR4302536.3360677/94‑1 (MQ=60)
                                   CTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCC                                                           >  SRR4302536.5616343/1‑94 (MQ=60)
                                   CTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCC                                                           >  SRR4302536.4180051/1‑94 (MQ=60)
                                    TGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCT                                                          <  SRR4302536.6785951/94‑1 (MQ=60)
                                    TGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCT                                                          >  SRR4302536.3867095/1‑94 (MQ=60)
                                     GATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTT                                                         <  SRR4302536.2852429/94‑1 (MQ=60)
                                      ATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTC                                                        >  SRR4302536.2351480/1‑94 (MQ=60)
                                        GGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCAT                                                      >  SRR4302536.4151003/1‑94 (MQ=60)
                                          AGAACTGTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAG                                                    <  SRR4302536.3655201/94‑1 (MQ=60)
                                          AGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAG                                                    <  SRR4302536.261123/94‑1 (MQ=60)
                                          AGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAG                                                    <  SRR4302536.2339173/94‑1 (MQ=60)
                                          AGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAG                                                    >  SRR4302536.3537009/1‑94 (MQ=60)
                                             ACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCAC                                                 <  SRR4302536.611276/94‑1 (MQ=60)
                                             ACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCAC                                                 >  SRR4302536.1910478/1‑94 (MQ=60)
                                              CTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACG                                                <  SRR4302536.2322694/94‑1 (MQ=60)
                                                CTGCTGGACGGGCTTATTTGTGTATGCATAAGGCCTATCTCTGGCAACCGTCTTCGATTGACCGCATCGATAGAATCCTCCTTCATAGCACGCT                                              <  SRR4302536.1980415/94‑1 (MQ=60)
                                                CTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGAT                                              <  SRR4302536.2656331/94‑1 (MQ=60)
                                                 TGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATG                                             >  SRR4302536.4192587/1‑94 (MQ=60)
                                                 TGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATG                                             <  SRR4302536.2314664/94‑1 (MQ=60)
                                                 TGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATG                                             <  SRR4302536.4879669/94‑1 (MQ=60)
                                                   CTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCC                                           >  SRR4302536.1245181/1‑94 (MQ=60)
                                                   CTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCC                                           <  SRR4302536.4756474/94‑1 (MQ=60)
                                                   CTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCC                                           <  SRR4302536.2407994/94‑1 (MQ=60)
                                                    TACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCT                                          >  SRR4302536.4017054/1‑94 (MQ=60)
                                                    TACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCT                                          <  SRR4302536.5132436/94‑1 (MQ=60)
                                                    TACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCT                                          <  SRR4302536.3667508/94‑1 (MQ=60)
                                                    TACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCT                                          >  SRR4302536.2648883/1‑94 (MQ=60)
                                                      CACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTT                                        <  SRR4302536.3940327/94‑1 (MQ=60)
                                                      CACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTT                                        >  SRR4302536.3749654/1‑94 (MQ=60)
                                                       ACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTC                                       >  SRR4302536.4510332/1‑94 (MQ=60)
                                                        CGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCA                                      <  SRR4302536.3226421/94‑1 (MQ=60)
                                                        CGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCA                                      <  SRR4302536.6106224/94‑1 (MQ=60)
                                                        CGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCA                                      <  SRR4302536.286385/94‑1 (MQ=60)
                                                         GGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCAC                                     <  SRR4302536.3252275/94‑1 (MQ=60)
                                                         GGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCAC                                     >  SRR4302536.4794420/1‑94 (MQ=60)
                                                          GGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACT                                    <  SRR4302536.1730000/94‑1 (MQ=60)
                                                          GGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACT                                    <  SRR4302536.3097388/94‑1 (MQ=60)
                                                              TATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATC                                >  SRR4302536.1667528/1‑94 (MQ=60)
                                                              TATTTTTTTATGCATAAGCCATATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATC                                <  SRR4302536.5780997/94‑1 (MQ=60)
                                                                TTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGG                              <  SRR4302536.670661/94‑1 (MQ=60)
                                                                TTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGG                              <  SRR4302536.3584522/94‑1 (MQ=60)
                                                                TTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTAACTTATCGG                              <  SRR4302536.6748583/94‑1 (MQ=60)
                                                                 TTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGC                             <  SRR4302536.5535031/94‑1 (MQ=60)
                                                                 TTTTTTATGCATAAGCACTATCTCGGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGC                             <  SRR4302536.1574966/94‑1 (MQ=60)
                                                                  TTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCA                            <  SRR4302536.1285972/94‑1 (MQ=60)
                                                                  TTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCA                            <  SRR4302536.3703732/94‑1 (MQ=60)
                                                                  TTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCA                            >  SRR4302536.505836/1‑94 (MQ=60)
                                                                   TTTTATGCATAAGCCCTGTCTCTGGCAACCGTCTTCCATTGACCACATGGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCAT                           <  SRR4302536.5079250/94‑1 (MQ=60)
                                                                    TTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATC                          >  SRR4302536.3124665/1‑94 (MQ=60)
                                                                     TTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCG                         <  SRR4302536.2370871/94‑1 (MQ=60)
                                                                      TATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGT                        <  SRR4302536.1054758/94‑1 (MQ=60)
                                                                      TATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGT                        >  SRR4302536.1412303/1‑94 (MQ=60)
                                                                      TATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGT                        <  SRR4302536.2781058/94‑1 (MQ=60)
                                                                      TATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGT                        >  SRR4302536.2866839/1‑94 (MQ=60)
                                                                       ATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTG                       >  SRR4302536.1407791/1‑94 (MQ=60)
                                                                         GCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCT                     <  SRR4302536.1878163/94‑1 (MQ=60)
                                                                         GCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCT                     <  SRR4302536.2410925/94‑1 (MQ=60)
                                                                         GCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCT                     <  SRR4302536.3374334/94‑1 (MQ=60)
                                                                         GCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCT                     <  SRR4302536.176306/94‑1 (MQ=60)
                                                                          CATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTC                    >  SRR4302536.2084766/1‑94 (MQ=60)
                                                                           ATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCC                   >  SRR4302536.3485596/1‑94 (MQ=60)
                                                                            TAAGCCTTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCC                  <  SRR4302536.6811230/94‑1 (MQ=60)
                                                                            TAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCC                  <  SRR4302536.4079918/94‑1 (MQ=60)
                                                                             TAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCA                 >  SRR4302536.2061976/1‑94 (MQ=60)
                                                                             AAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCA                 <  SRR4302536.2890339/94‑1 (MQ=60)
                                                                              AGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCAC                >  SRR4302536.2429851/1‑94 (MQ=60)
                                                                               GCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACA               >  SRR4302536.651393/1‑94 (MQ=60)
                                                                               GCCCTATCTCTGGCAACCGTATTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACA               >  SRR4302536.6737728/1‑94 (MQ=60)
                                                                                CCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACCG              >  SRR4302536.2408538/1‑94 (MQ=60)
                                                                                CCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAG              >  SRR4302536.6418285/1‑94 (MQ=60)
                                                                                CCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAG              >  SRR4302536.5895328/1‑94 (MQ=60)
                                                                                ACCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCACACAG              >  SRR4302536.2657782/1‑94 (MQ=60)
                                                                                 CCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGG             >  SRR4302536.3600246/1‑94 (MQ=60)
                                                                                 CCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGG             >  SRR4302536.216580/1‑94 (MQ=60)
                                                                                  CTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGT            >  SRR4302536.4825406/1‑94 (MQ=60)
                                                                                  CTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGT            <  SRR4302536.4010007/94‑1 (MQ=60)
                                                                                  CTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGT            >  SRR4302536.3928018/1‑94 (MQ=60)
                                                                                  CTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGT            >  SRR4302536.3606073/1‑94 (MQ=60)
                                                                                  CTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGT            <  SRR4302536.5919815/94‑1 (MQ=60)
                                                                                   TATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTT           >  SRR4302536.829840/1‑94 (MQ=60)
                                                                                   TATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTT           <  SRR4302536.1972284/94‑1 (MQ=60)
                                                                                   TATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTT           <  SRR4302536.5900961/94‑1 (MQ=60)
                                                                                   TATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTT           >  SRR4302536.6631406/1‑94 (MQ=60)
                                                                                   TATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTT           <  SRR4302536.3912728/94‑1 (MQ=60)
                                                                                    ATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTC          >  SRR4302536.5531641/1‑94 (MQ=60)
                                                                                    ATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCGTTCACTTATCGGCATCGGGCTCCCACACGTTC          >  SRR4302536.1872078/1‑94 (MQ=60)
                                                                                     TCTCTGGCAACCGTCTTGCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTAGCGGCATCGTGCTCCCACAGGTTCC         <  SRR4302536.5330464/94‑1 (MQ=60)
                                                                                      CTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCG        >  SRR4302536.6687139/1‑94 (MQ=60)
                                                                                      CTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCG        >  SRR4302536.4614971/1‑94 (MQ=60)
                                                                                      CTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCG        >  SRR4302536.4234738/1‑94 (MQ=60)
                                                                                      CTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCG        <  SRR4302536.6581069/94‑1 (MQ=60)
                                                                                         TGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCT     <  SRR4302536.6839545/94‑1 (MQ=60)
                                                                                         TGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCT     <  SRR4302536.2551417/94‑1 (MQ=60)
                                                                                          GGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTA    >  SRR4302536.4702271/1‑94 (MQ=60)
                                                                                           GAAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTAC   <  SRR4302536.6126476/94‑1 (MQ=60)
                                                                                            CAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  >  SRR4302536.1777784/1‑94 (MQ=60)
                                                                                            CAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  >  SRR4302536.292060/1‑94 (MQ=60)
                                                                                            CAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  <  SRR4302536.4738387/94‑1 (MQ=60)
                                                                                            |                                                                                             
TTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  >  NC_000913/2165782‑2165967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑