Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,204,258	T→C	W182R (TGG→CGG)	yehM →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,204,258	0	T	C	100.0%	405.1 / NA	125	W182R (TGG→CGG)	yehM	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (62/63); total (62/63)

Only 100 of 140 total aligned reads displayed.

CCCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCTGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGC  >  NC_000913/2204168‑2204347

cccTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcgg                                                                                         >  1:1811281/1‑93 (MQ=255)

cccTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcgg                                                                                         >  1:1811280/1‑93 (MQ=255)

 ccTCTGCGCTGGCGCCCTATTTTGCCCAGCTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcgga                                                                                        <  2:1775233/93‑1 (MQ=255)

 ccTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcgga                                                                                        >  2:422321/1‑93 (MQ=255)

  ctctGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAGGCCTTTATGGCCCGCcggat                                                                                       >  1:1545348/1‑93 (MQ=255)

      gcgcTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcggattgga                                                                                   <  2:3520493/93‑1 (MQ=255)

       cgcTGGCGCAGTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcggattggat                                                                                  >  2:3056250/1‑93 (MQ=255)

       cgcTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcggattggat                                                                                  <  2:36375/93‑1 (MQ=255)

        gcTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATg                                                                                 <  1:3107690/93‑1 (MQ=255)

          tGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATggg                                                                               <  2:1187334/93‑1 (MQ=255)

           ggCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGc                                                                              >  2:713958/1‑93 (MQ=255)

              gcACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGGTGGGCGAt                                                                           >  1:3774029/1‑93 (MQ=255)

               ccgtATTTGGACCAACGGCGGGGCCACGCCTGCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATg                                                                          <  1:1951837/90‑1 (MQ=255)

                aCTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATgc                                                                         <  2:3924671/93‑1 (MQ=255)

                aCTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATgc                                                                         >  1:3583611/1‑93 (MQ=255)

                  tATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATgcag                                                                       >  1:3657712/1‑93 (MQ=255)

                  tATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATgcag                                                                       >  2:1617790/1‑93 (MQ=255)

                   aTTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATgcagc                                                                      >  1:4171838/1‑93 (MQ=255)

                   aTTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATgcagc                                                                      >  2:766927/1‑93 (MQ=255)

                       tGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCaaaa                                                                  >  1:3627516/1‑93 (MQ=255)

                        gCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAaat                                                                 >  2:4307708/1‑93 (MQ=255)

                        gCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAaat                                                                 <  1:604592/93‑1 (MQ=255)

                        gCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAaat                                                                 <  1:4033064/93‑1 (MQ=255)

                          ccAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAaataa                                                               <  2:1552571/93‑1 (MQ=255)

                          ccAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAaataa                                                               >  2:2076477/1‑93 (MQ=255)

                          ccAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAaataa                                                               >  1:919319/1‑93 (MQ=255)

                           cAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAaataat                                                              <  2:2650715/93‑1 (MQ=255)

                            aaCTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATg                                                             <  1:2837704/93‑1 (MQ=255)

                            aaCTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATg                                                             >  1:1973227/1‑93 (MQ=255)

                            aaCTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATg                                                             <  2:181886/93‑1 (MQ=255)

                             aCTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATgg                                                            <  2:4125191/93‑1 (MQ=255)

                              cTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGc                                                           <  2:660810/93‑1 (MQ=255)

                              cTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGc                                                           <  1:3174069/93‑1 (MQ=255)

                               tGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCg                                                          >  2:3901633/1‑93 (MQ=255)

                               tGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCg                                                          >  1:2697110/1‑93 (MQ=255)

                                 ctgggCGACGCCTCCGGCGATGCGCTCAATCGCCAGCCCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGAc                                                        <  2:1081147/91‑1 (MQ=255)

                                 cGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGAc                                                        <  2:1081148/93‑1 (MQ=255)

                                  ggggCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGAATGGCCGCGGCACCAAAATAATGCCCACg                                                       >  1:1457185/1‑93 (MQ=255)

                                   gggCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACgt                                                      <  1:3472411/93‑1 (MQ=255)

                                   gggCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACgt                                                      <  1:652488/93‑1 (MQ=255)

                                    ggCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACgtg                                                     <  2:4234000/93‑1 (MQ=255)

                                    ggCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACgtg                                                     <  1:1635276/93‑1 (MQ=255)

                                     gCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACgtgt                                                    <  2:105372/93‑1 (MQ=255)

                                      cGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGtt                                                   >  1:1152985/1‑93 (MQ=255)

                                       gACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTa                                                  >  2:3706028/1‑93 (MQ=255)

                                       gACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTa                                                  >  2:2697534/1‑93 (MQ=255)

                                       gACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTa                                                  <  2:2121941/93‑1 (MQ=255)

                                        aCGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAg                                                 >  1:1045207/1‑93 (MQ=255)

                                          gCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATTGGCGATGCAGCAAAATAATGGCGACGTGTTAGtt                                               >  2:4596529/1‑93 (MQ=255)

                                          gCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGtt                                               <  2:3465243/93‑1 (MQ=255)

                                           ccTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGGTAGTTg                                              >  2:1794383/1‑93 (MQ=255)

                                            cTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGt                                             >  1:3245896/1‑93 (MQ=255)

                                            cTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGt                                             >  2:4747890/1‑93 (MQ=255)

                                             tCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTc                                            <  1:480817/93‑1 (MQ=255)

                                             tCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTc                                            <  2:1928580/93‑1 (MQ=255)

                                             tCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTc                                            <  1:271341/93‑1 (MQ=255)

                                              ccGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCt                                           <  1:2094212/93‑1 (MQ=255)

                                                  cGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCgg                                       <  2:3425940/93‑1 (MQ=255)

                                                   gATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGt                                      <  2:940460/93‑1 (MQ=255)

                                                   gATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGt                                      <  1:3424208/93‑1 (MQ=255)

                                                   gATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGt                                      >  1:350564/1‑93 (MQ=255)

                                                    aTGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGtg                                     <  1:2371052/93‑1 (MQ=255)

                                                      gcgcTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGtggc                                   <  1:295652/93‑1 (MQ=255)

                                                         cTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGtggctgg                                >  1:3097090/1‑93 (MQ=255)

                                                          tCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGtggctggc                               >  2:216029/1‑93 (MQ=255)

                                                          tCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGtggctggc                               <  1:3904182/93‑1 (MQ=255)

                                                           cAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCa                              >  1:2485085/1‑93 (MQ=255)

                                                           cAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCa                              <  1:1875119/93‑1 (MQ=255)

                                                                gTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTc                         >  1:2379473/1‑93 (MQ=255)

                                                                gTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTc                         >  1:2552898/1‑93 (MQ=255)

                                                                  cAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCg                       >  1:2809882/1‑93 (MQ=255)

                                                                  cAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCg                       >  2:2832571/1‑93 (MQ=255)

                                                                   aGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCgg                      >  2:4730271/1‑93 (MQ=255)

                                                                   aGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCgg                      >  2:594847/1‑93 (MQ=255)

                                                                      gcgAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCAc                   <  1:3654440/93‑1 (MQ=255)

                                                                      gcgAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCAc                   <  2:2803962/93‑1 (MQ=255)

                                                                       cgAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACt                  <  1:2337245/93‑1 (MQ=255)

                                                                       cgAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACt                  <  2:4533130/93‑1 (MQ=255)

                                                                          aGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTgga               >  1:984180/1‑92 (MQ=255)

                                                                          aGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGc               >  2:1676846/1‑93 (MQ=255)

                                                                          aGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGc               >  2:1526779/1‑93 (MQ=255)

                                                                           gCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCa              >  1:3568497/1‑93 (MQ=255)

                                                                           gCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCa              <  2:4377443/93‑1 (MQ=255)

                                                                            ccTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCaa             >  2:4258673/1‑93 (MQ=255)

                                                                              tttATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAg           >  1:1914056/1‑93 (MQ=255)

                                                                              tttATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAg           >  1:1586069/1‑93 (MQ=255)

                                                                               ttATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGCCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGa          <  2:1121646/93‑1 (MQ=255)

                                                                                tATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGAt         <  2:1460032/93‑1 (MQ=255)

                                                                                 aTGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGAtg        <  1:1066584/93‑1 (MQ=255)

                                                                                 aTGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGAtg        >  2:2266129/1‑93 (MQ=255)

                                                                                 aTGGCCCGACGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGAtg        >  2:3092323/1‑93 (MQ=255)

                                                                                   ggCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGAtgtg      <  2:432737/93‑1 (MQ=255)

                                                                                   ggCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGAtgtg      >  2:2984395/1‑93 (MQ=255)

                                                                                   ggCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGAtgtg      >  1:4478305/1‑93 (MQ=255)

                                                                                   ggCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGAtgtg      >  1:128243/1‑93 (MQ=255)

                                                                                    gCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTgg     >  1:3078748/1‑93 (MQ=255)

                                                                                    gCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTgg     >  2:3296069/1‑93 (MQ=255)

                                                                                    gCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTgg     <  1:4724466/93‑1 (MQ=255)

                                                                                     cccGCCTGATTTGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGgc    <  2:3551472/93‑1 (MQ=255)

                                                                                       cGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGgcgc  >  1:639361/1‑93 (MQ=255)

CCCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCTGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGC  >  NC_000913/2204168‑2204347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TACCCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCTGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGCGAAT  >  NC_000913/2204166‑2204351

TACCCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCG                                                                                              <  SRR4302544.3475018/94‑1 (MQ=60)

 ACCCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGG                                                                                             <  SRR4302544.4760772/94‑1 (MQ=60)

 ACCCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGG                                                                                             >  SRR4302544.1828969/1‑94 (MQ=60)

 ACCCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGG                                                                                             >  SRR4302544.1828970/1‑94 (MQ=60)

  CCCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGNNAAGCCTTTATGGCCCGCCNGA                                                                                            >  SRR4302544.528242/1‑94 (MQ=60)

   CCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAGGCCTTTATGGCCCGCCGGAT                                                                                           >  SRR4302544.1560739/1‑94 (MQ=60)

          GCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGG                                                                                    <  SRR4302544.3137269/94‑1 (MQ=60)

            TGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGC                                                                                  <  SRR4302544.3539275/94‑1 (MQ=60)

               CGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGGTGGGCGAT                                                                               >  SRR4302544.3811431/1‑94 (MQ=60)

                 ccgtatttggaCCAACGGCGGGGCCACGCCTGCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGC                                                                             <  SRR4302544.1970762/83‑1 (MQ=60)

                 CACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGC                                                                             <  SRR4302544.1970767/94‑1 (MQ=60)

                 CACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGC                                                                             >  SRR4302544.3618000/1‑94 (MQ=60)

                   CTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAG                                                                           >  SRR4302544.3628835/1‑94 (MQ=60)

                   CTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAG                                                                           >  SRR4302544.3694200/1‑94 (MQ=60)

                   CTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAG                                                                           >  SRR4302544.4050169/1‑94 (MQ=60)

                    TATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGC                                                                          >  SRR4302544.4213163/1‑94 (MQ=60)

                    TATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGC                                                                          <  SRR4302544.3040660/94‑1 (MQ=60)

                        TTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAA                                                                      >  SRR4302544.3663655/1‑94 (MQ=60)

                         TGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAAT                                                                     >  SRR4302544.2432553/1‑94 (MQ=60)

                          GCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATA                                                                    <  SRR4302544.4073238/94‑1 (MQ=60)

                          GCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATA                                                                    <  SRR4302544.610525/94‑1 (MQ=60)

                           CCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAA                                                                   >  SRR4302544.928395/1‑94 (MQ=60)

                             CAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATG                                                                 >  SRR4302544.1992406/1‑94 (MQ=60)

                              AAGTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGAGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGG                                                                <  SRR4302544.2865108/94‑1 (MQ=60)

                              AACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGG                                                                <  SRR4302544.2865109/94‑1 (MQ=60)

                                CTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCG                                                              <  SRR4302544.3204274/94‑1 (MQ=60)

                                CTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCG                                                              >  SRR4302544.2723016/1‑94 (MQ=60)

                                   CGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGaatggccgcggcaccaaaataatgcccacg                                                           >  SRR4302544.1471655/1‑64 (MQ=60)

                                   CGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACG                                                           >  SRR4302544.1471656/1‑94 (MQ=60)

                                     GGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTG                                                         <  SRR4302544.658948/94‑1 (MQ=60)

                                     GGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTG                                                         <  SRR4302544.3505528/94‑1 (MQ=60)

                                     GGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTG                                                         <  SRR4302544.3356646/94‑1 (MQ=60)

                                      GGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGT                                                        <  SRR4302544.1651372/94‑1 (MQ=60)

                                       GCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTT                                                       >  SRR4302544.1164448/1‑94 (MQ=60)

                                         GACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAG                                                     >  SRR4302544.1055573/1‑94 (MQ=60)

                                           ccccgCCGGCGATGCGCTCAATCGGCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTT                                                   <  SRR4302544.1540890/89‑1 (MQ=60)

                                             CCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGT                                                 >  SRR4302544.3276967/1‑94 (MQ=60)

                                               TCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCT                                               <  SRR4302544.274199/94‑1 (MQ=60)

                                               TCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCT                                               <  SRR4302544.4719792/94‑1 (MQ=60)

                                               TCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCT                                               <  SRR4302544.485524/94‑1 (MQ=60)

                                                CCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTG                                              <  SRR4302544.2114367/94‑1 (MQ=60)

                                                    CGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGT                                          >  SRR4302544.354158/1‑94 (MQ=60)

                                                     GATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTG                                         <  SRR4302544.3456943/94‑1 (MQ=60)

                                                      ATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGG                                        <  SRR4302544.2393488/94‑1 (MQ=60)

                                                        GCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCT                                      <  SRR4302544.298710/94‑1 (MQ=60)

                                                          GCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGG                                    >  SRR4302544.3126582/1‑94 (MQ=60)

                                                            TCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCA                                  >  SRR4302544.2508882/1‑94 (MQ=60)

                                                            TCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCA                                  <  SRR4302544.3942971/94‑1 (MQ=60)

                                                             CAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCAC                                 <  SRR4302544.1893247/94‑1 (MQ=60)

                                                                TCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCT                              <  SRR4302544.3169041/94‑1 (MQ=60)

                                                                 CGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCCCGCTC                             >  SRR4302544.3753290/1‑94 (MQ=60)

                                                                 CGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTC                             >  SRR4302544.2577407/1‑94 (MQ=60)

                                                                 CGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTC                             >  SRR4302544.2402005/1‑94 (MQ=60)

                                                                 CGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTC                             >  SRR4302544.2704262/1‑94 (MQ=60)

                                                                 CGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTATGCGGTGGCTGGCACGCTC                             >  SRR4302544.2704261/1‑94 (MQ=60)

                                                                   TCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCG                           >  SRR4302544.2836993/1‑94 (MQ=60)

                                                                    CAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGG                          <  SRR4302544.800668/94‑1 (MQ=60)

                                                                      GCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCA                        >  SRR4302544.2247554/1‑94 (MQ=60)

                                                                        GCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACT                      <  SRR4302544.3690905/94‑1 (MQ=60)

                                                                         CGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTG                     <  SRR4302544.2359394/94‑1 (MQ=60)

                                                                           AAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGA                   >  SRR4302544.993828/1‑94 (MQ=60)

                                                                            AGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCA                  >  SRR4302544.3602714/1‑94 (MQ=60)

                                                                               CTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAG               >  SRR4302544.1932552/1‑94 (MQ=60)

                                                                               CTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAG               >  SRR4302544.1601724/1‑94 (MQ=60)

                                                                                   ATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGT           <  SRR4302544.1077192/94‑1 (MQ=60)

                                                                                    TGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTG          >  SRR4302544.4523328/1‑94 (MQ=60)

                                                                                    TGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTG          <  SRR4302544.1710386/94‑1 (MQ=60)

                                                                                    TGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTG          >  SRR4302544.129424/1‑94 (MQ=60)

                                                                                     GGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGG         >  SRR4302544.3108079/1‑94 (MQ=60)

                                                                                     GGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGG         <  SRR4302544.1874709/94‑1 (MQ=60)

                                                                                      GCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGC        <  SRR4302544.4771103/94‑1 (MQ=60)

                                                                                       CCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCG       <  SRR4302544.1240508/94‑1 (MQ=60)

                                                                                        CCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGC      >  SRR4302544.645663/1‑94 (MQ=60)

                                                                                            CGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGCGAAT  >  SRR4302544.4373530/1‑94 (MQ=60)

                                                                                            CGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGCGAAT  <  SRR4302544.212740/94‑1 (MQ=60)

TACCCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCTGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGCGAAT  >  NC_000913/2204166‑2204351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`