Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,208,331 A→G S404S (TCA→TCG)  b2122 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,208,3310AG99.1% 327.8 / ‑4.4 105S404S (TCA→TCG) b2122hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (30/74);  total (30/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

Only 100 of 128 total aligned reads displayed.

GCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG  >  NC_000913/2208241‑2208420
                                                                                          |                                                                                         
gCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTcgca                                                                                         <  2:2545722/93‑1 (MQ=255)
 ccGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCaa                                                                                        <  1:1686054/93‑1 (MQ=255)
  cgcgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAag                                                                                       <  2:4733474/93‑1 (MQ=255)
   gcgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAaga                                                                                      >  2:1662575/1‑92 (MQ=255)
    cgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAagca                                                                                     <  2:323795/93‑1 (MQ=255)
    cgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAagca                                                                                     <  1:2775173/93‑1 (MQ=255)
     gAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGGCAATTGTTTTGCcagcag                                                                                    >  2:227762/1‑93 (MQ=255)
     gAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCCAAATTTTTCGCaaaccg                                                                                    >  2:2123450/1‑89 (MQ=255)
     gAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGCGCAAATTGTTTCGCAaacag                                                                                    >  1:812963/1‑93 (MQ=255)
      aaGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAagcagc                                                                                   <  2:2710134/93‑1 (MQ=255)
        gTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCCAATTGGTTTGCAAGGAGCaa                                                                                 >  2:2203228/1‑93 (MQ=255)
        gTGAAAAGGAGAACTCGCCGGCAGATGCGCGTAGGCTGTTTAGTTTTCAGGCTGGCGCTCTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCaa                                                                                 <  2:496057/93‑1 (MQ=255)
        atGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCaa                                                                                 <  1:1380932/92‑1 (MQ=255)
           aaCAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGAGCACTGGCGGGCGGGCAAATTGCTTGGCAAGGAGCAAAAc                                                                              >  1:1860541/1‑93 (MQ=255)
            aCAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACg                                                                             >  2:383052/1‑93 (MQ=255)
              aGGAAACCGCACCTGCAGCGACGCGTCGGCGGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTGCCGCAAGCAGCAAAACgaa                                                                           <  2:3871744/93‑3 (MQ=255)
               ggAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGGCAATTGTTTCGCAAGCAGCAAAACGCAg                                                                          >  1:4329369/1‑93 (MQ=255)
               ggAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAg                                                                          <  2:3882838/93‑1 (MQ=255)
                gAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGt                                                                         <  1:3839263/93‑1 (MQ=255)
                gAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGt                                                                         <  1:3143576/93‑1 (MQ=255)
                gAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTCTCACGCTGGCGCACTGGCGGGCGGGCGAACTGTTTCTGCAGGCGCAAAACGCacg                                                                         >  1:2092184/1‑91 (MQ=255)
                 aaaaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTg                                                                        <  2:803726/93‑1 (MQ=255)
                 aaaaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTg                                                                        <  2:803725/93‑1 (MQ=255)
                 aaaaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTg                                                                        <  1:3276956/93‑1 (MQ=255)
                   aaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGcc                                                                      <  2:4685889/93‑1 (MQ=255)
                   aaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGcc                                                                      <  1:320995/93‑1 (MQ=255)
                   aaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAACAAAACGCAGTGcc                                                                      >  2:2298319/1‑93 (MQ=255)
                      tCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTTGCCAGCCACAAAACGCAGTGCCGAt                                                                   >  2:2015518/1‑93 (MQ=255)
                      tCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAt                                                                   <  2:3560684/93‑1 (MQ=255)
                      tCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAt                                                                   <  1:3570560/93‑1 (MQ=255)
                       cACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCCAGCAGCAAAAAGCAGTGCCGATg                                                                  >  1:1268676/1‑93 (MQ=255)
                       cACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATg                                                                  <  1:156968/93‑1 (MQ=255)
                         ccGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGc                                                                >  2:1375445/1‑93 (MQ=255)
                         ccGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGc                                                                <  1:1948796/93‑1 (MQ=255)
                           ggCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTTGCAAGCAGCAAAACGCAGTGCCGATGGCgg                                                              >  1:2566742/1‑92 (MQ=255)
                           ggCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGa                                                              <  2:545257/93‑1 (MQ=255)
                            gCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAg                                                             <  1:597015/93‑1 (MQ=255)
                            gCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGGCGTGCCGATGGCGAg                                                             >  2:3227303/1‑93 (MQ=255)
                             cAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAgc                                                            <  2:3267971/93‑1 (MQ=255)
                              aGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAgct                                                           <  1:3249557/93‑1 (MQ=255)
                               gCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAgctg                                                          >  2:2562985/1‑93 (MQ=255)
                               gCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAgctg                                                          >  2:1622297/1‑93 (MQ=255)
                                    gcgTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGtt                                                     <  1:2038134/93‑1 (MQ=255)
                                    gcgTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGtt                                                     >  2:1907107/1‑93 (MQ=255)
                                      gTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAg                                                   >  1:3540352/1‑93 (MQ=255)
                                      gTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAACAGCAAAACGCAGTGCCGATCGCGAGCTGCTGTTAg                                                   >  2:760700/1‑93 (MQ=255)
                                       tCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGc                                                  <  1:3025961/93‑1 (MQ=255)
                                       tCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGc                                                  <  2:1188281/93‑1 (MQ=255)
                                        tggCTTTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCt                                                 <  2:503811/92‑1 (MQ=255)
                                        cGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCt                                                 >  1:1879235/1‑93 (MQ=255)
                                         ggCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTa                                                >  1:2974894/1‑93 (MQ=255)
                                           cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAGATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              >  2:1572475/1‑93 (MQ=255)
                                           cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              <  1:1890300/93‑1 (MQ=255)
                                           cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              <  1:2966041/93‑1 (MQ=255)
                                           cTGTTTAGTTTTCAGGCCGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              <  2:3124796/93‑1 (MQ=255)
                                            tGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTTTTAGCTActc                                             >  1:2296241/1‑91 (MQ=255)
                                            tGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAccc                                             <  1:1264757/93‑1 (MQ=255)
                                              tttAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGc                                           >  1:4677880/1‑93 (MQ=255)
                                                tAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCaa                                         <  1:1044258/93‑1 (MQ=255)
                                                    gttCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGc                                     <  2:2168559/92‑1 (MQ=255)
                                                     ttCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCt                                    <  1:4422786/93‑1 (MQ=255)
                                                     ttCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCt                                    <  1:4383110/93‑1 (MQ=255)
                                                        aGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTaa                                 >  1:1048430/1‑93 (MQ=255)
                                                        aGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTaa                                 <  1:938778/93‑1 (MQ=255)
                                                          gCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagc                               <  2:1678582/93‑1 (MQ=255)
                                                          gCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagc                               <  1:789848/93‑1 (MQ=255)
                                                            tGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcag                             <  2:3675756/93‑1 (MQ=255)
                                                             ggCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcagc                            <  1:3256042/93‑1 (MQ=255)
                                                               cgcACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGt                          <  1:4051306/93‑1 (MQ=255)
                                                                 cACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtg                        >  1:1073037/1‑93 (MQ=255)
                                                                  aCTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtgt                       >  1:1769961/1‑93 (MQ=255)
                                                                   cTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtgtg                      >  1:2744514/1‑93 (MQ=255)
                                                                    tGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGc                     >  1:1481373/1‑93 (MQ=255)
                                                                    tGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGc                     <  1:3754357/93‑1 (MQ=255)
                                                                       cgggcgggCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCGGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGc                  <  2:2827124/93‑1 (MQ=255)
                                                                       cgggcgggCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGc                  >  2:2336054/1‑93 (MQ=255)
                                                                       cgggcgggCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGc                  <  2:2369379/93‑1 (MQ=255)
                                                                        gggcgggcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAATCGCTTAAGCAGCGTTGTGCCGCt                 >  2:3241608/1‑93 (MQ=255)
                                                                        gggcgggcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCt                 <  2:2675504/93‑1 (MQ=255)
                                                                         ggcgggcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTg                >  2:222421/1‑93 (MQ=255)
                                                                          gcgggcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGt               <  1:158647/93‑1 (MQ=255)
                                                                          gcgggcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGt               <  2:3100328/93‑1 (MQ=255)
                                                                          gcgggcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGt               <  2:4033421/93‑1 (MQ=255)
                                                                           cgggcAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTc              <  1:3816851/93‑1 (MQ=255)
                                                                            gggcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCg             >  2:1337505/1‑93 (MQ=255)
                                                                              gcAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGcc           <  1:1111832/93‑1 (MQ=255)
                                                                                aaaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTg         >  1:1783191/1‑93 (MQ=255)
                                                                                aaaTTGTTTCGAAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTg         <  1:4303827/93‑1 (MQ=255)
                                                                                 aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        >  2:2719767/1‑93 (MQ=255)
                                                                                 aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        >  1:1605920/1‑93 (MQ=255)
                                                                                  aTTGTTTCGCAAGCAGCAAAACGCAGTGCGGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAt       <  2:1971876/93‑1 (MQ=255)
                                                                                   ttGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATg      >  2:1185448/1‑93 (MQ=255)
                                                                                   ttGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATg      >  2:3054362/1‑93 (MQ=255)
                                                                                   ttGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATg      <  1:2639220/93‑1 (MQ=255)
                                                                                     gTTTCGCAAGCAGCACACCGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    <  2:4204615/93‑1 (MQ=255)
                                                                                      tttCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCTTTGTGCCGCTGTCGCCTGATGCCt   <  2:4686810/93‑1 (MQ=255)
                                                                                      tttCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCt   <  2:4257414/93‑1 (MQ=255)
                                                                                       ttCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTg  <  1:498685/93‑1 (MQ=255)
                                                                                       ttCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTg  <  1:3573/93‑1 (MQ=255)
                                                                                       ttCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTg  <  2:1921225/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG  >  NC_000913/2208241‑2208420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGG  >  NC_000913/2208238‑2208421
                                                                                             |                                                                                          
tttgtctCGAAGTTAACAGGAAAACTCACCGGGAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCG                                                                                            <  SRR4302544.1665873/87‑1 (MQ=60)
 TGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGC                                                                                           >  SRR4302544.2579994/1‑94 (MQ=60)
    CCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAG                                                                                        <  SRR4302544.1890763/94‑1 (MQ=60)
    CCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAG                                                                                        <  SRR4302544.1702675/94‑1 (MQ=60)
       CGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAG                                                                                     <  SRR4302544.2801865/94‑1 (MQ=60)
       CGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGCGCAAATTGTTTCGCAAACAG                                                                                     >  SRR4302544.820899/1‑94 (MQ=60)
         AAGTCAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTTGCAAGCAGCA                                                                                   >  SRR4302544.1178805/1‑94 (MQ=60)
           GTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAA                                                                                 <  SRR4302544.4428414/94‑1 (MQ=60)
           ATGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAA                                                                                 <  SRR4302544.1394719/94‑1 (MQ=60)
             GAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGAGCACTGGCGGGCGGGCAAATTGCTTGGCAAGGAGCAAAAC                                                                               >  SRR4302544.1878528/1‑94 (MQ=60)
                 AGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGGCAATTGTTTCGCAAGCAGCAAAACGCAG                                                                           >  SRR4302544.4373457/1‑94 (MQ=60)
                   GAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTG                                                                         <  SRR4302544.3877344/94‑1 (MQ=60)
                   GAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTG                                                                         <  SRR4302544.3173462/94‑1 (MQ=60)
                    AAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGC                                                                        <  SRR4302544.3308187/94‑1 (MQ=60)
                      AACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCG                                                                      <  SRR4302544.324330/94‑1 (MQ=60)
                         TCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCCAGCAGCAAAAAGCAGTGCCGATG                                                                   >  SRR4302544.1281153/1‑94 (MQ=60)
                         TCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATG                                                                   <  SRR4302544.3604798/94‑1 (MQ=60)
                          CACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGG                                                                  <  SRR4302544.158506/94‑1 (MQ=60)
                            CCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCG                                                                <  SRR4302544.1967669/94‑1 (MQ=60)
                             CGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTTGCAAGCAGCAAAACGCAGTGCCGATGGCGG                                                               >  SRR4302544.2591403/1‑94 (MQ=60)
                              cggaggTACGCGTCTGCTGTTTAGTGTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAG                                                              <  SRR4302544.1657146/88‑1 (MQ=60)
                              GGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAG                                                              <  SRR4302544.1545964/94‑1 (MQ=60)
                              GGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAG                                                              <  SRR4302544.1657145/94‑1 (MQ=60)
                               GCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGC                                                             <  SRR4302544.602866/94‑1 (MQ=60)
                                 AGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTG                                                           <  SRR4302544.3280650/94‑1 (MQ=60)
                                   CTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCT                                                         >  SRR4302544.3410728/1‑94 (MQ=60)
                                     ACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGT                                                       <  SRR4302544.1994886/94‑1 (MQ=60)
                                       GCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTA                                                     <  SRR4302544.2057820/94‑1 (MQ=60)
                                        CGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAG                                                    >  SRR4302544.3574234/1‑94 (MQ=60)
                                          TCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCT                                                  >  SRR4302544.1897406/1‑94 (MQ=60)
                                          TCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCT                                                  <  SRR4302544.3054951/94‑1 (MQ=60)
                                           CGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGGTGGCGAGGTGCTGTTAGCTA                                                 >  SRR4302544.695308/1‑94 (MQ=60)
                                           CGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTA                                                 >  SRR4302544.3003431/1‑94 (MQ=60)
                                              CTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTTTTAGCTACTC                                              >  SRR4302544.2317893/1‑94 (MQ=60)
                                              CTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCC                                              <  SRR4302544.2994498/94‑1 (MQ=60)
                                              CTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCC                                              <  SRR4302544.1908562/94‑1 (MQ=60)
                                               TGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCG                                             <  SRR4302544.1277203/94‑1 (MQ=60)
                                               TGTTTAGTTTTCAGGCTAGCGCACTGGCGGTCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAGGGCGAGCTGCTGTTAGCTACCCG                                             <  SRR4302544.1403316/94‑1 (MQ=60)
                                                GTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGC                                            >  SRR4302544.4724125/1‑94 (MQ=60)
                                                GTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGCTTCGCCAGCAGCAAAACGCAGTGCCGATTGCGAGCTGCTGTTAGCTACCCGC                                            >  SRR4302544.1948060/1‑94 (MQ=60)
                                                   TAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAAC                                         <  SRR4302544.1054617/94‑1 (MQ=60)
                                                    AGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTGCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACC                                        <  SRR4302544.2885677/94‑1 (MQ=60)
                                                     GTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCG                                       <  SRR4302544.3234570/94‑1 (MQ=60)
                                                        TTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTT                                    <  SRR4302544.4427500/94‑1 (MQ=60)
                                                        TTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTT                                    <  SRR4302544.4467407/94‑1 (MQ=60)
                                                          CAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAA                                  >  SRR4302544.1058820/1‑94 (MQ=60)
                                                           AGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAG                                 <  SRR4302544.948025/94‑1 (MQ=60)
                                                             GCTGGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCA                               <  SRR4302544.797487/94‑1 (MQ=60)
                                                                GGCGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCG                            <  SRR4302544.3287172/94‑1 (MQ=60)
                                                                  CGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTT                          <  SRR4302544.4091653/94‑1 (MQ=60)
                                                                  CGCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTT                          <  SRR4302544.4234775/94‑1 (MQ=60)
                                                                   GCACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTG                         >  SRR4302544.1083706/1‑94 (MQ=60)
                                                                    CACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGT                        >  SRR4302544.1787285/1‑94 (MQ=60)
                                                                     ACTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTG                       >  SRR4302544.2770962/1‑94 (MQ=60)
                                                                      CTGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGC                      >  SRR4302544.1496011/1‑94 (MQ=60)
                                                                       TGGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCC                     <  SRR4302544.3791632/94‑1 (MQ=60)
                                                                        GGCGGGCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCG                    >  SRR4302544.2654699/1‑94 (MQ=60)
                                                                            GCCGGGCAAATGGTTTCGCAAGCAGCAAAACGCAGTGACGATGGCGAGCTGCTGTTAGCTCCCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGT                <  SRR4302544.2903874/94‑1 (MQ=60)
                                                                             GCGGGCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTC               <  SRR4302544.160199/94‑1 (MQ=60)
                                                                              CGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCG              <  SRR4302544.3854687/94‑1 (MQ=60)
                                                                                 GCAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCT           <  SRR4302544.1122924/94‑1 (MQ=60)
                                                                                  CAAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTG          >  SRR4302544.1800629/1‑94 (MQ=60)
                                                                                   AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGA         >  SRR4302544.1621750/1‑94 (MQ=60)
                                                                                   AAATTGTTTCGAAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGA         <  SRR4302544.4347722/94‑1 (MQ=60)
                                                                                      TTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGC      <  SRR4302544.2664519/94‑1 (MQ=60)
                                                                                      TTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGC      <  SRR4302544.3648574/94‑1 (MQ=60)
                                                                                          TTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGG  <  SRR4302544.503584/94‑1 (MQ=60)
                                                                                          TTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGG  <  SRR4302544.3604/94‑1 (MQ=60)
                                                                                             |                                                                                          
TTGGCCGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTGG  >  NC_000913/2208238‑2208421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑