Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,208,505 G→A R462R (CGG→CGA)  b2122 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,208,5050GA98.5% 460.0 / ‑4.6 136R462R (CGG→CGA) b2122hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (62/72);  total (64/72)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01

Only 100 of 140 total aligned reads displayed.

TGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAT  >  NC_000913/2208415‑2208592
                                                                                          |                                                                                       
tGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGAcc                                                                                       >  2:1664161/1‑93 (MQ=255)
  ccTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGTAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACctc                                                                                     <  2:2036706/93‑1 (MQ=255)
   cTGGCAGATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACctct                                                                                    <  1:3108818/93‑1 (MQ=255)
   cTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACctct                                                                                    <  1:2956821/93‑1 (MQ=255)
    tGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCtt                                                                                   >  1:321626/1‑93 (MQ=255)
      gCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTaa                                                                                 <  2:3811764/93‑1 (MQ=255)
      gCAAATGTGGAGCGCGCCGTTACGCCAGCCGGGGATTGTGCCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTaa                                                                                 <  2:1756485/93‑1 (MQ=255)
       cAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAAt                                                                                <  1:3453464/93‑1 (MQ=255)
       cAAAGGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAAt                                                                                <  1:160093/93‑1 (MQ=255)
           tGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAgg                                                                            <  1:2203228/93‑1 (MQ=255)
           tGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAgg                                                                            >  2:2946849/1‑93 (MQ=255)
            tttGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGt                                                                           <  2:3832668/92‑1 (MQ=255)
            gTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGt                                                                           <  1:281262/93‑1 (MQ=255)
                agggcgcCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGAt                                                                       <  1:1240459/90‑1 (MQ=255)
                aGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGAt                                                                       >  2:2944310/1‑93 (MQ=255)
                 gcgcgcCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATa                                                                      >  2:1678302/1‑93 (MQ=255)
                 gcgcgcCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATa                                                                      >  1:3908466/1‑93 (MQ=255)
                   gcgcCGTTCCGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAAc                                                                    <  2:1268676/93‑1 (MQ=255)
                   gcgcCGTTACGCCAGCCGGGCATTGTGGCTTTGCTGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAAc                                                                    <  2:1541294/93‑1 (MQ=255)
                   gcgcCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAAc                                                                    <  1:2562985/93‑1 (MQ=255)
                   cccccGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAAc                                                                    <  1:1622297/90‑1 (MQ=255)
                      ccGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACtta                                                                 <  2:4156408/93‑1 (MQ=255)
                      ccGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACtta                                                                 >  1:1368745/1‑93 (MQ=255)
                       cGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttat                                                                >  1:1499607/1‑93 (MQ=255)
                        gTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttatt                                                               <  2:4677880/93‑1 (MQ=255)
                          tACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttattta                                                             <  2:4456326/93‑1 (MQ=255)
                           aCGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttatttat                                                            <  2:4329369/93‑1 (MQ=255)
                             gCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTc                                                          <  1:66730/93‑1 (MQ=255)
                             gCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTc                                                          <  1:3494099/93‑1 (MQ=255)
                              ccAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCt                                                         <  1:2600985/93‑1 (MQ=255)
                              ccAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCt                                                         <  2:2223602/93‑1 (MQ=255)
                                aGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGc                                                       >  1:282447/1‑93 (MQ=255)
                                aGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGc                                                       <  2:1642796/93‑1 (MQ=255)
                                   cGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCgg                                                    >  1:2586248/1‑93 (MQ=255)
                                   cGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCgg                                                    >  1:182159/1‑93 (MQ=255)
                                   cGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCgg                                                    >  2:136801/1‑93 (MQ=255)
                                   cGGGCATTGTGGCTTTGCGGAATTATTTCCGCCAGCTCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCgg                                                    <  1:3371026/93‑1 (MQ=255)
                                    gggCATTGGGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGt                                                   <  1:3508404/93‑1 (MQ=255)
                                     ggCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTc                                                  >  1:3427556/1‑93 (MQ=255)
                                     ggCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTc                                                  >  2:3015360/1‑93 (MQ=255)
                                      gCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCg                                                 <  1:2997777/93‑1 (MQ=255)
                                      gCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCg                                                 >  2:1384741/1‑93 (MQ=255)
                                          tgtgGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGa                                             <  1:4342753/93‑1 (MQ=255)
                                            tgGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAgt                                           <  2:1048430/93‑1 (MQ=255)
                                             ggCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAgtg                                          >  2:22491/1‑93 (MQ=255)
                                              gCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTATGCCGGTCGCTGAgtgt                                         <  1:475165/93‑1 (MQ=255)
                                               cTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTa                                        <  1:227762/93‑1 (MQ=255)
                                                tttGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAt                                       >  1:3414325/1‑93 (MQ=255)
                                                tttGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAt                                       <  1:1375445/93‑1 (MQ=255)
                                                tttGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAt                                       <  2:4171011/93‑1 (MQ=255)
                                                 ttGCGGGAATATTTCCGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAtt                                      <  2:3378481/93‑1 (MQ=255)
                                                     gggAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGc                                  >  1:1977861/1‑93 (MQ=255)
                                                     gggAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATGTCGc                                  >  1:2058145/1‑93 (MQ=255)
                                                      ggAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCt                                 <  1:4053518/93‑1 (MQ=255)
                                                      ggAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCt                                 >  2:148333/1‑93 (MQ=255)
                                                      ggAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCt                                 <  1:744921/93‑1 (MQ=255)
                                                         atatTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCTGTCGCTGAGTGTATTTCGCTCgg                              >  2:2608368/1‑93 (MQ=255)
                                                         atatTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCgg                              <  1:3780704/93‑1 (MQ=255)
                                                         atatTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCgg                              <  1:2204895/93‑1 (MQ=255)
                                                          tatTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGt                             <  1:733370/93‑1 (MQ=255)
                                                          tatTTACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGt                             <  1:4101415/93‑1 (MQ=255)
                                                           atTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGtt                            <  1:3922633/93‑1 (MQ=255)
                                                           atTTACGCCAGCGTCCCCCCGACTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGtt                            >  2:3990608/1‑93 (MQ=255)
                                                            tttACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTACTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTg                           >  1:4572293/1‑93 (MQ=255)
                                                               aCGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGa                        >  2:1882763/1‑93 (MQ=255)
                                                                cGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAc                       <  2:2566742/93‑1 (MQ=255)
                                                                cGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAc                       <  1:52440/93‑1 (MQ=255)
                                                                 ccccgcgcCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTTGCTCGGTTGGGAca                      <  2:3564581/89‑1 (MQ=255)
                                                                   cAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcagc                    <  2:103186/93‑1 (MQ=255)
                                                                    aGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGGTGGGAcagca                   >  1:1802590/1‑93 (MQ=255)
                                                                     gCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcagcag                  >  2:2014741/1‑93 (MQ=255)
                                                                     gCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcagcag                  >  1:4680905/1‑93 (MQ=255)
                                                                     gCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcagcag                  >  1:1951361/1‑93 (MQ=255)
                                                                      cgcccccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACagcagc                 >  2:1009051/4‑93 (MQ=255)
                                                                      cGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACagcagc                 <  2:571506/93‑1 (MQ=255)
                                                                       gTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAgcc                >  2:4392569/1‑93 (MQ=255)
                                                                          cccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAgccgcc             >  2:1809345/1‑93 (MQ=255)
                                                                          cccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAgccgcc             >  2:399698/1‑93 (MQ=255)
                                                                           ccccTCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCa            <  2:3097075/93‑1 (MQ=255)
                                                                           ccccGCCTGCATCCGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCATCCGCCa            <  2:3097074/93‑1 (MQ=255)
                                                                           ccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCa            <  1:215748/93‑1 (MQ=255)
                                                                            cccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAg           >  1:4678614/1‑93 (MQ=255)
                                                                             ccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGa          >  1:4359957/1‑93 (MQ=255)
                                                                             ccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGCTTGCGACAGCAGCCGCCAGa          >  2:1267415/1‑93 (MQ=255)
                                                                              cGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGAc         <  1:1518705/93‑1 (MQ=255)
                                                                               gCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACg        >  2:3756997/1‑93 (MQ=255)
                                                                               gCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACg        >  2:4088146/1‑93 (MQ=255)
                                                                                 cTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCt      >  1:1554759/1‑93 (MQ=255)
                                                                                 cTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCt      >  1:2554795/1‑93 (MQ=255)
                                                                                 cTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCt      >  1:3245043/1‑93 (MQ=255)
                                                                                 cTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCt      <  1:373191/93‑1 (MQ=255)
                                                                                 cTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCt      <  1:1185448/93‑1 (MQ=255)
                                                                                  tGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTg     <  2:2555457/93‑1 (MQ=255)
                                                                                  tGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTg     >  1:2694368/1‑93 (MQ=255)
                                                                                   gCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTgg    >  1:2420509/1‑93 (MQ=255)
                                                                                   gCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTgg    >  2:3625926/1‑93 (MQ=255)
                                                                                   gCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTgg    <  1:1156094/93‑1 (MQ=255)
                                                                                     aTACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAt  <  1:2298319/93‑1 (MQ=255)
                                                                                     aTACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAt  <  2:3540352/93‑1 (MQ=255)
                                                                                     aTACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAt  >  2:1293356/1‑93 (MQ=255)
                                                                                          |                                                                                       
TGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAT  >  NC_000913/2208415‑2208592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATG  >  NC_000913/2208417‑2208593
                                                                                        |                                                                                        
GCTGGGAAATGTTGAGCGCGCCGTTAGACCAGCCGGGCATTGTGGCTTTGCGGGAATCTTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCT                                                                                     <  SRR4302544.851293/94‑1 (MQ=60)
CCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCT                                                                                     <  SRR4302544.132993/94‑1 (MQ=60)
CCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCT                                                                                     >  SRR4302544.4171350/1‑94 (MQ=60)
 CTGGCAGATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTT                                                                                    <  SRR4302544.3138403/94‑1 (MQ=60)
 CTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTT                                                                                    <  SRR4302544.2985214/94‑1 (MQ=60)
 CTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTT                                                                                    >  SRR4302544.324966/1‑94 (MQ=60)
    GCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAAT                                                                                 >  SRR4302544.2103549/1‑94 (MQ=60)
     CAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATC                                                                                <  SRR4302544.3486434/94‑1 (MQ=60)
     CAAAGGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATC                                                                                <  SRR4302544.161657/94‑1 (MQ=60)
         TGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGT                                                                            <  SRR4302544.2223980/94‑1 (MQ=60)
          GTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTC                                                                           <  SRR4302544.284195/94‑1 (MQ=60)
              AGGGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATA                                                                       <  SRR4302544.1252707/94‑1 (MQ=60)
              AGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATA                                                                       >  SRR4302544.3947300/1‑94 (MQ=60)
              AGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAACCAGGTCGATA                                                                       <  SRR4302544.2631672/94‑1 (MQ=60)
                CGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAAC                                                                     <  SRR4302544.2393103/94‑1 (MQ=60)
                 cccCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACT                                                                    <  SRR4302544.1638281/91‑1 (MQ=60)
                 GCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACT                                                                    <  SRR4302544.2587612/94‑1 (MQ=60)
                   GCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTA                                                                  >  SRR4302544.1382354/1‑94 (MQ=60)
                    CCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTAT                                                                 >  SRR4302544.1514436/1‑94 (MQ=60)
                     CGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATT                                                                <  SRR4302544.2963422/94‑1 (MQ=60)
                      GTTGCGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTT                                                               <  SRR4302544.3093373/94‑1 (MQ=60)
                           GCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCT                                                          <  SRR4302544.67398/94‑1 (MQ=60)
                           GCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCT                                                          <  SRR4302544.3527415/94‑1 (MQ=60)
                            CCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTG                                                         <  SRR4302544.2625984/94‑1 (MQ=60)
                             CAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGC                                                        >  SRR4302544.285395/1‑94 (MQ=60)
                                CCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGG                                                     >  SRR4302544.183960/1‑94 (MQ=60)
                                CCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGG                                                     >  SRR4302544.2611122/1‑94 (MQ=60)
                                 CGGGCATTGTGGCTTTGCGGAATTATTTCCGCCAGCTCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGT                                                    <  SRR4302544.3403211/94‑1 (MQ=60)
                                  GGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTC                                                   >  SRR4302544.3460323/1‑94 (MQ=60)
                                  GGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTC                                                   <  SRR4302544.69628/94‑1 (MQ=60)
                                  GGGCATTGGGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTC                                                   <  SRR4302544.3541875/94‑1 (MQ=60)
                                    GCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGC                                                 <  SRR4302544.3026521/94‑1 (MQ=60)
                                       TTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGA                                              >  SRR4302544.1479348/1‑94 (MQ=60)
                                        TGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAG                                             <  SRR4302544.4386925/94‑1 (MQ=60)
                                           GGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGT                                          <  SRR4302544.3302195/94‑1 (MQ=60)
                                           GGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGT                                          >  SRR4302544.1603171/1‑94 (MQ=60)
                                            GCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTATGCCGGTCGCTGAGTGTA                                         <  SRR4302544.479815/94‑1 (MQ=60)
                                             CTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAT                                        >  SRR4302544.3446958/1‑94 (MQ=60)
                                             CTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAT                                        <  SRR4302544.230125/94‑1 (MQ=60)
                                              TTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATT                                       <  SRR4302544.1389155/94‑1 (MQ=60)
                                                 GCGGGAATATTTACGCCGGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCG                                    <  SRR4302544.224722/94‑1 (MQ=60)
                                                 GCGGGAATATTTACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCG                                    <  SRR4302544.866219/94‑1 (MQ=60)
                                                  CGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGC                                   >  SRR4302544.1997080/1‑94 (MQ=60)
                                                  CGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATGTCGC                                   >  SRR4302544.2077944/1‑94 (MQ=60)
                                                   GGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCT                                  <  SRR4302544.3530413/94‑1 (MQ=60)
                                                    GGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTC                                 <  SRR4302544.752212/94‑1 (MQ=60)
                                                    GGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTC                                 <  SRR4302544.4093887/94‑1 (MQ=60)
                                                       ATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGT                              >  SRR4302544.4692152/1‑94 (MQ=60)
                                                       ATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGT                              <  SRR4302544.2225659/94‑1 (MQ=60)
                                                       ATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGT                              <  SRR4302544.3818150/94‑1 (MQ=60)
                                                        TATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTT                             <  SRR4302544.740539/94‑1 (MQ=60)
                                                        TATTTACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTT                             <  SRR4302544.4142222/94‑1 (MQ=60)
                                                         ATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTG                            <  SRR4302544.3961597/94‑1 (MQ=60)
                                                         ATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTACTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTG                            >  SRR4302544.4617860/1‑94 (MQ=60)
                                                           TTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGG                          >  SRR4302544.3806927/1‑94 (MQ=60)
                                                              CGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACA                       <  SRR4302544.52971/94‑1 (MQ=60)
                                                                CCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGC                     >  SRR4302544.346092/1‑94 (MQ=60)
                                                                 CAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGGTGGGACAGCA                    >  SRR4302544.1820198/1‑94 (MQ=60)
                                                                  AGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAG                   >  SRR4302544.4727185/1‑94 (MQ=60)
                                                                  AGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAG                   >  SRR4302544.1984551/1‑94 (MQ=60)
                                                                  AGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAG                   >  SRR4302544.1970280/1‑94 (MQ=60)
                                                                     GTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCG                <  SRR4302544.261134/94‑1 (MQ=60)
                                                                       CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCTGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCC              >  SRR4302544.3984009/1‑94 (MQ=60)
                                                                         CCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAG            <  SRR4302544.217973/94‑1 (MQ=60)
                                                                         CCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAG            >  SRR4302544.4724869/1‑94 (MQ=60)
                                                                          CCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGA           >  SRR4302544.4404205/1‑94 (MQ=60)
                                                                            CGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACG         <  SRR4302544.1533780/94‑1 (MQ=60)
                                                                              CCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCT       >  SRR4302544.1570227/1‑94 (MQ=60)
                                                                              CCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCT       >  SRR4302544.2579324/1‑94 (MQ=60)
                                                                              CCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCT       >  SRR4302544.3276110/1‑94 (MQ=60)
                                                                               CTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTG      <  SRR4302544.377005/94‑1 (MQ=60)
                                                                               CTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTG      >  SRR4302544.1806066/1‑94 (MQ=60)
                                                                               CTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTG      >  SRR4302544.2720234/1‑94 (MQ=60)
                                                                               CTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTG      <  SRR4302544.1197233/94‑1 (MQ=60)
                                                                                TGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGG     >  SRR4302544.2443560/1‑94 (MQ=60)
                                                                                 GCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGA    <  SRR4302544.1167598/94‑1 (MQ=60)
                                                                                   ATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATG  <  SRR4302544.2320007/94‑1 (MQ=60)
                                                                                   ATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATG  <  SRR4302544.4773822/94‑1 (MQ=60)
                                                                                        |                                                                                        
CCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATG  >  NC_000913/2208417‑2208593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑