Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,208,505 G→A R462R (CGG→CGA)  b2122 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,208,5050GA99.6% 823.5 / ‑5.2 231R462R (CGG→CGA) b2122hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (107/123);  total (107/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.68e-01

Only 100 of 238 total aligned reads displayed.

TGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGC  >  NC_000913/2208415‑2208594
                                                                                          |                                                                                         
 gCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACct                                                                                        >  2:1181790/1‑93 (MQ=255)
 gCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACct                                                                                        >  1:6539447/1‑93 (MQ=255)
 gCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACct                                                                                        <  2:324525/93‑1 (MQ=255)
  ccTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACctc                                                                                       <  2:6703797/93‑1 (MQ=255)
  ccTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACctc                                                                                       <  2:4793528/93‑1 (MQ=255)
  ccTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACctc                                                                                       >  1:2335293/1‑93 (MQ=255)
   cTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACctct                                                                                      <  2:4505864/93‑1 (MQ=255)
   cTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACctct                                                                                      >  1:916329/1‑93 (MQ=255)
         aaTGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCa                                                                                <  2:2688445/93‑1 (MQ=255)
          aTGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAg                                                                               <  1:2102172/93‑1 (MQ=255)
           tGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAgg                                                                              <  2:6582127/93‑1 (MQ=255)
           tGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAgg                                                                              >  2:1759427/1‑93 (MQ=255)
            gtggAGCGCGCCTTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGt                                                                             <  1:1162064/90‑1 (MQ=255)
            gTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGt                                                                             >  2:5189863/1‑93 (MQ=255)
            gTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGt                                                                             <  1:1072022/93‑1 (MQ=255)
            gTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGt                                                                             <  1:3920095/93‑1 (MQ=255)
               gAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGa                                                                          <  2:2301153/93‑1 (MQ=255)
                aGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGAt                                                                         >  2:3699227/1‑93 (MQ=255)
                aGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGAt                                                                         <  1:3314785/93‑1 (MQ=255)
                 gcgcgcCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATa                                                                        <  2:5493650/93‑1 (MQ=255)
                   gcgcCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAAc                                                                      >  2:5550990/1‑93 (MQ=255)
                   gcgcCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAAc                                                                      <  1:1548170/93‑1 (MQ=255)
                   gcgcCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAAc                                                                      <  2:2151069/93‑1 (MQ=255)
                    cgccgcTACTCCAGCCGGGCATTGTGGCTTGGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACt                                                                     <  1:639652/93‑1 (MQ=255)
                    cgcCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACt                                                                     <  1:3512840/93‑1 (MQ=255)
                      ctgTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACtta                                                                   <  2:2057729/91‑1 (MQ=255)
                      ccGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACtta                                                                   <  2:5864842/93‑1 (MQ=255)
                       cGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttat                                                                  <  2:4296342/93‑1 (MQ=255)
                        gTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttatt                                                                 >  2:5698444/1‑93 (MQ=255)
                        gTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttatt                                                                 <  2:5601453/93‑1 (MQ=255)
                        gTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttatt                                                                 <  2:2761369/93‑1 (MQ=255)
                        gTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttatt                                                                 <  2:3201192/93‑1 (MQ=255)
                        gTTACGCCACGCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATAGGACCTCTTAATCAGGTCGATAACttatt                                                                 <  2:309344/93‑1 (MQ=255)
                          tACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttattta                                                               <  1:5457288/93‑1 (MQ=255)
                           cccccAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACttatttat                                                              <  1:4764290/90‑1 (MQ=255)
                           aCGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACtaatttat                                                              >  1:3817199/1‑93 (MQ=255)
                            cGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTtatttatt                                                             >  2:4809497/1‑93 (MQ=255)
                            cGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTtatttatt                                                             <  1:401555/93‑1 (MQ=255)
                                aGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGc                                                         <  1:32031/93‑1 (MQ=255)
                                 gCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGcc                                                        >  1:5640683/1‑93 (MQ=255)
                                 gCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGcc                                                        >  2:6461980/1‑93 (MQ=255)
                                 gCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGcc                                                        >  1:3274070/1‑93 (MQ=255)
                                 gCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCACCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGcc                                                        >  1:596401/1‑93 (MQ=255)
                                   cGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCgg                                                      <  1:460100/93‑1 (MQ=255)
                                    gggCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGt                                                     >  1:5868225/1‑93 (MQ=255)
                                     ggagttGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTc                                                    <  1:6144420/89‑1 (MQ=255)
                                     ggCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTc                                                    <  1:4849438/93‑1 (MQ=255)
                                       cATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGc                                                  >  2:4515686/1‑93 (MQ=255)
                                       cATTGTGGCTTTGCGGAAATATTTGCGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGc                                                  <  2:433921/93‑1 (MQ=255)
                                        aTTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCt                                                 >  1:6617563/1‑93 (MQ=255)
                                        aTTGTGGCTTTGCGGGAATATTTACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCt                                                 <  1:4588225/93‑1 (MQ=255)
                                         ttGTGGCTTTGCGGGATTATTTACGACAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTg                                                <  2:200109/93‑1 (MQ=255)
                                         ttGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTg                                                >  2:1512452/1‑93 (MQ=255)
                                           gtgGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAg                                              <  2:2357671/93‑1 (MQ=255)
                                             ggCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAgtg                                            <  2:1054158/93‑1 (MQ=255)
                                             ggCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAgtg                                            >  2:1636290/1‑93 (MQ=255)
                                               cTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTa                                          >  2:1835935/1‑93 (MQ=255)
                                               cTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTa                                          >  1:422040/1‑93 (MQ=255)
                                                tttGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAt                                         <  2:3961146/93‑1 (MQ=255)
                                                 ttGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAtt                                        <  2:814929/93‑1 (MQ=255)
                                                 ttGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAtt                                        <  2:5055398/93‑1 (MQ=255)
                                                 ttGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAtt                                        <  2:6389194/93‑1 (MQ=255)
                                                  tGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAttt                                       <  1:1852751/93‑1 (MQ=255)
                                                   gCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTc                                      <  1:2634529/93‑1 (MQ=255)
                                                    cGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCg                                     <  1:1797531/93‑1 (MQ=255)
                                                     gggAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTNTTTCGc                                    >  2:5686189/1‑93 (MQ=255)
                                                      ggAATATTTACGCCTGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCt                                   >  1:4068065/1‑93 (MQ=255)
                                                       gAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTTGCTGAGTGTATTTCGCTc                                  <  1:3130951/93‑1 (MQ=255)
                                                         atatTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCgg                                >  1:4148291/1‑93 (MQ=255)
                                                          tatTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGt                               <  1:5913636/93‑1 (MQ=255)
                                                          tatTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGt                               <  2:5177885/93‑1 (MQ=255)
                                                          tatTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGt                               >  1:1447527/1‑93 (MQ=255)
                                                            tttACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTg                             >  1:1507273/1‑93 (MQ=255)
                                                                cGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAc                         >  1:2647407/1‑93 (MQ=255)
                                                                  cccgcgcCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcag                       <  1:3460528/90‑1 (MQ=255)
                                                                   cAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcagc                      >  1:2199105/1‑93 (MQ=255)
                                                                    aGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcagca                     <  2:2492680/93‑1 (MQ=255)
                                                                    aGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAcagca                     >  1:5034189/1‑93 (MQ=255)
                                                                      gctCCCCCCGCCTGCATACGTCCTCGTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACagcagc                   <  1:3006911/91‑1 (MQ=255)
                                                                        tCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACagcagcgg                 >  1:5418903/1‑91 (MQ=255)
                                                                        tCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAgccg                 >  2:384166/1‑93 (MQ=255)
                                                                         ccccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAgccgc                >  2:461306/1‑93 (MQ=255)
                                                                          cccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAgccgcc               >  2:1367252/1‑93 (MQ=255)
                                                                          cccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAgccgcc               <  2:3030883/93‑1 (MQ=255)
                                                                          cccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAgccgcc               <  1:6659185/93‑1 (MQ=255)
                                                                           ccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCa              >  2:4428488/1‑93 (MQ=255)
                                                                           ccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCa              >  1:2988106/1‑93 (MQ=255)
                                                                           ccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCa              >  1:827246/1‑93 (MQ=255)
                                                                           ccccGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCa              <  1:4393597/93‑1 (MQ=255)
                                                                              cGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGAc           <  1:4034977/93‑1 (MQ=255)
                                                                               gCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACg          >  2:6489093/1‑93 (MQ=255)
                                                                                ccTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGc         >  2:3347960/1‑93 (MQ=255)
                                                                                ccTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGc         >  1:1602168/1‑93 (MQ=255)
                                                                                ccTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGc         >  1:5735504/1‑93 (MQ=255)
                                                                                   gCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTgg      <  2:3464125/93‑1 (MQ=255)
                                                                                     aTACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAt    <  2:6030484/93‑1 (MQ=255)
                                                                                     aTACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAt    >  2:2945062/1‑93 (MQ=255)
                                                                                     aTACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGAt    <  1:6269703/93‑1 (MQ=255)
                                                                                      tACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATg   >  2:1447747/1‑93 (MQ=255)
                                                                                       aCGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATgc  <  1:2418668/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGC  >  NC_000913/2208415‑2208594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAG  >  NC_000913/2208412‑2208598
                                                                                             |                                                                                             
TGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGA                                                                                               >  SRR4302536.6005903/1‑94 (MQ=60)
  ATGCCTGGCAAATGTTGAGCGCCCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACC                                                                                             <  SRR4302536.1257455/94‑1 (MQ=60)
   TGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCT                                                                                            <  SRR4302536.3380448/94‑1 (MQ=60)
   TGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCT                                                                                            >  SRR4302536.3202904/1‑94 (MQ=60)
   TGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCT                                                                                            >  SRR4302536.6734213/1‑94 (MQ=60)
    GCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTC                                                                                           >  SRR4302536.6689295/1‑94 (MQ=60)
    GCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTC                                                                                           >  SRR4302536.2406148/1‑94 (MQ=60)
     CCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCT                                                                                          >  SRR4302536.6133951/1‑94 (MQ=60)
     CCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCT                                                                                          >  SRR4302536.943341/1‑94 (MQ=60)
      CTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTT                                                                                         >  SRR4302536.683095/1‑94 (MQ=60)
      CTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTT                                                                                         >  SRR4302536.853008/1‑94 (MQ=60)
        GGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAA                                                                                       >  SRR4302536.1360224/1‑94 (MQ=60)
         GGAAATGTTGAGCGCGCCGTTACGCCAGCGGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAAT                                                                                      <  SRR4302536.5676423/94‑1 (MQ=60)
          CAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATC                                                                                     <  SRR4302536.4738367/94‑1 (MQ=60)
             ATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGG                                                                                  <  SRR4302536.2165376/94‑1 (MQ=60)
             ATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGG                                                                                  >  SRR4302536.3616597/1‑94 (MQ=60)
              TGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGT                                                                                 >  SRR4302536.291478/1‑94 (MQ=60)
               GTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTC                                                                                <  SRR4302536.4036611/94‑1 (MQ=60)
               GTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTC                                                                                <  SRR4302536.1104102/94‑1 (MQ=60)
               GTGGAGCGCGCCTTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTC                                                                                <  SRR4302536.1197043/94‑1 (MQ=60)
                 TGAGCGCGCCGTTACGCCAGCCGGGCATTGAGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGA                                                                              >  SRR4302536.2298133/1‑94 (MQ=60)
                   AGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATA                                                                            <  SRR4302536.3412762/94‑1 (MQ=60)
                   AGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATA                                                                            <  SRR4302536.3917656/94‑1 (MQ=60)
                   AGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATA                                                                            <  SRR4302536.3762504/94‑1 (MQ=60)
                      GCGCGGTCACCCCAGCCGGGCATTGTGGCTTTGCGGGAATATTCCCGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACT                                                                         <  SRR4302536.6357714/94‑1 (MQ=60)
                      GCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACT                                                                         <  SRR4302536.1593681/94‑1 (MQ=60)
                      CCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGGCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACT                                                                         <  SRR4302536.3408296/94‑1 (MQ=60)
                       CGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTT                                                                        >  SRR4302536.4394337/1‑94 (MQ=60)
                       CGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTT                                                                        <  SRR4302536.3617729/94‑1 (MQ=60)
                       CGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTT                                                                        <  SRR4302536.1591539/94‑1 (MQ=60)
                       CGCCGCTACTCCAGCCGGGCATTGTGGCTTGGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTT                                                                        <  SRR4302536.658532/94‑1 (MQ=60)
                        GCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTA                                                                       >  SRR4302536.644616/1‑94 (MQ=60)
                        GCCGTTACGCCAGCCGGGCATTGTGGCTTTGC‑GGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTAT                                                                      >  SRR4302536.4680358/1‑94 (MQ=60)
                         CCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTAT                                                                      <  SRR4302536.1107448/94‑1 (MQ=60)
                         CCGTAACGCCAGCCGGGAATGGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTAT                                                                      <  SRR4302536.1679181/94‑1 (MQ=60)
                          cgtacgGCCAGCGGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATT                                                                     <  SRR4302536.3087241/88‑1 (MQ=60)
                          cgcgcCGCCAGCGGGGCTTTGTGGCTTTGCGGGAATATTAACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATT                                                                     <  SRR4302536.5989682/89‑1 (MQ=60)
                            TTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTA                                                                   <  SRR4302536.3320778/94‑1 (MQ=60)
                             TACGCCAGCGGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTAT                                                                  <  SRR4302536.5901625/94‑1 (MQ=60)
                             TACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTAT                                                                  <  SRR4302536.5620465/94‑1 (MQ=60)
                             TACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTAT                                                                  <  SRR4302536.4146146/94‑1 (MQ=60)
                             TACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTAATTTAT                                                                  >  SRR4302536.3930963/1‑94 (MQ=60)
                              cccCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATT                                                                 <  SRR4302536.4907771/91‑1 (MQ=60)
                              TCGCCAGCGGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATT                                                                 <  SRR4302536.4181928/94‑1 (MQ=60)
                              ACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATT                                                                 <  SRR4302536.2148838/94‑1 (MQ=60)
                               CGCCAGCGGGACATTGTGGCTTTGCGGGAATATTTACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTC                                                                <  SRR4302536.6170729/94‑1 (MQ=60)
                               CGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTC                                                                <  SRR4302536.412176/94‑1 (MQ=60)
                                GCCAGCGGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCT                                                               <  SRR4302536.6079954/94‑1 (MQ=60)
                                 CCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTG                                                              <  SRR4302536.1932612/94‑1 (MQ=60)
                                 CCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTG                                                              >  SRR4302536.4214219/1‑94 (MQ=60)
                                   AGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCC                                                            <  SRR4302536.32921/94‑1 (MQ=60)
                                   AGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCC                                                            >  SRR4302536.3370252/1‑94 (MQ=60)
                                   AGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCC                                                            >  SRR4302536.5808267/1‑94 (MQ=60)
                                   AGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCACCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCC                                                            >  SRR4302536.613029/1‑94 (MQ=60)
                                    GCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCG                                                           <  SRR4302536.5589269/94‑1 (MQ=60)
                                    GCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCG                                                           <  SRR4302536.967139/94‑1 (MQ=60)
                                      CGGGCTTTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGT                                                         <  SRR4302536.408323/94‑1 (MQ=60)
                                      CGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGT                                                         >  SRR4302536.3530266/1‑94 (MQ=60)
                                      CGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGT                                                         >  SRR4302536.6043080/1‑94 (MQ=60)
                                      CGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGT                                                         <  SRR4302536.472125/94‑1 (MQ=60)
                                        ggagTTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCG                                                       <  SRR4302536.6327497/90‑1 (MQ=60)
                                        GGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCG                                                       <  SRR4302536.4995358/94‑1 (MQ=60)
                                        GGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCG                                                       >  SRR4302536.1515605/1‑94 (MQ=60)
                                        GGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCG                                                       >  SRR4302536.422215/1‑94 (MQ=60)
                                          CATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCT                                                     >  SRR4302536.6814309/1‑94 (MQ=60)
                                           ATTGTGGCTTTGCGGGAATATTTACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTG                                                    <  SRR4302536.4725961/94‑1 (MQ=60)
                                             TGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAG                                                  >  SRR4302536.3089173/1‑94 (MQ=60)
                                              GTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGT                                                 <  SRR4302536.6752190/94‑1 (MQ=60)
                                                GGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGT                                               <  SRR4302536.4868642/94‑1 (MQ=60)
                                                 GCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTA                                              >  SRR4302536.433169/1‑94 (MQ=60)
                                                  CTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAT                                             >  SRR4302536.185861/1‑94 (MQ=60)
                                                  CTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAT                                             >  SRR4302536.5996050/1‑94 (MQ=60)
                                                  CTTTGCGGGAATATTTACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTAT                                             <  SRR4302536.2238458/94‑1 (MQ=60)
                                                    TTGAGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTT                                           <  SRR4302536.4282021/94‑1 (MQ=60)
                                                     TGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTC                                          <  SRR4302536.4850037/94‑1 (MQ=60)
                                                     TGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTC                                          <  SRR4302536.1908592/94‑1 (MQ=60)
                                                      GCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCG                                         <  SRR4302536.2713538/94‑1 (MQ=60)
                                                       CGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGC                                        <  SRR4302536.1851792/94‑1 (MQ=60)
                                                        GGGAATATTTACGCCTGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCT                                       >  SRR4302536.4188641/1‑94 (MQ=60)
                                                         GGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTC                                      <  SRR4302536.6056220/94‑1 (MQ=60)
                                                          GAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTTGCTGAGTGTATTTCGCTCG                                     <  SRR4302536.3222495/94‑1 (MQ=60)
                                                          GAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCG                                     >  SRR4302536.3247676/1‑94 (MQ=60)
                                                          GAATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCG                                     <  SRR4302536.1654828/94‑1 (MQ=60)
                                                          GAATATTTACGCCAGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCG                                     <  SRR4302536.686801/94‑1 (MQ=60)
                                                           AATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGG                                    >  SRR4302536.4077405/1‑94 (MQ=60)
                                                           AATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGG                                    >  SRR4302536.4271383/1‑94 (MQ=60)
                                                            ATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGT                                   >  SRR4302536.1490420/1‑94 (MQ=60)
                                                            ATATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGT                                   >  SRR4302536.152914/1‑94 (MQ=60)
                                                             TATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTT                                  <  SRR4302536.6089776/94‑1 (MQ=60)
                                                             TATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTT                                  <  SRR4302536.3033369/94‑1 (MQ=60)
                                                              ATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTG                                 >  SRR4302536.5179001/1‑94 (MQ=60)
                                                              ATTTACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTG                                 >  SRR4302536.1551686/1‑94 (MQ=60)
                                                                 TACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGA                              >  SRR4302536.5772016/1‑94 (MQ=60)
                                                                  ACGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGAC                             >  SRR4302536.2726720/1‑94 (MQ=60)
                                                                   CGCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACA                            >  SRR4302536.5469928/1‑94 (MQ=60)
                                                                    GCCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAAATTATTTATTCTGCCGGTCGCTGAGTGGATTTCGCTCCGTTCGGACAA                           >  SRR4302536.4005382/1‑94 (MQ=60)
                                                                     CCCGCGCCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGC                          <  SRR4302536.3563643/94‑1 (MQ=60)
                                                                     CCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGC                          >  SRR4302536.789136/1‑94 (MQ=60)
                                                                     CCAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGC                          >  SRR4302536.2265491/1‑94 (MQ=60)
                                                                      CAGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCA                         >  SRR4302536.5185083/1‑94 (MQ=60)
                                                                       agctcCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAG                        <  SRR4302536.4087270/89‑1 (MQ=60)
                                                                       CGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAG                        <  SRR4302536.1620177/94‑1 (MQ=60)
                                                                       AGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAG                        <  SRR4302536.6555783/94‑1 (MQ=60)
                                                                       AGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAG                        <  SRR4302536.5294628/94‑1 (MQ=60)
                                                                       AGCGTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGGTGGGACAGCAG                        >  SRR4302536.6405550/1‑94 (MQ=60)
                                                                       AGCGGCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAG                        <  SRR4302536.3146784/94‑1 (MQ=60)
                                                                         gcTCCCCCCGCCTGCATACGTCCTCGTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCC                      <  SRR4302536.3095308/92‑1 (MQ=60)
                                                                          GTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCGG                     >  SRR4302536.5580890/1‑94 (MQ=60)
                                                                          GTCCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCG                     >  SRR4302536.2425818/1‑94 (MQ=60)
                                                                            CCCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGACGCC                   >  SRR4302536.4916894/1‑94 (MQ=60)
                                                                             CCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCA                  >  SRR4302536.3076032/1‑94 (MQ=60)
                                                                             CCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCA                  >  SRR4302536.851600/1‑94 (MQ=60)
                                                                             CCCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCA                  <  SRR4302536.6857094/94‑1 (MQ=60)
                                                                              CCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAG                 >  SRR4302536.1019733/1‑94 (MQ=60)
                                                                              CCCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAG                 <  SRR4302536.4524843/94‑1 (MQ=60)
                                                                               CCCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCagcgaccggcagaataaataag                <  SRR4302536.2982147/94‑23 (MQ=60)
                                                                                CCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGAC               <  SRR4302536.2465421/94‑1 (MQ=60)
                                                                                CCGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGAC               <  SRR4302536.4507058/94‑1 (MQ=60)
                                                                                 CGCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACG              <  SRR4302536.4154634/94‑1 (MQ=60)
                                                                                  GCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGC             >  SRR4302536.1648921/1‑94 (MQ=60)
                                                                                  GCCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGC             >  SRR4302536.5906108/1‑94 (MQ=60)
                                                                                   CCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCT            <  SRR4302536.5633900/94‑1 (MQ=60)
                                                                                   CCTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCT            >  SRR4302536.2717105/1‑94 (MQ=60)
                                                                                    CTGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTG           >  SRR4302536.2542520/1‑94 (MQ=60)
                                                                                     TGCATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGG          >  SRR4302536.6721495/1‑94 (MQ=60)
                                                                                        ATACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATG       <  SRR4302536.6456500/94‑1 (MQ=60)
                                                                                          ACGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCG     <  SRR4302536.2491818/94‑1 (MQ=60)
                                                                                           CGACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGC    >  SRR4302536.2679406/1‑94 (MQ=60)
                                                                                             ACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAG  >  SRR4302536.6464521/1‑94 (MQ=60)
                                                                                             ACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAG  >  SRR4302536.692498/1‑94 (MQ=60)
                                                                                             ACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAG  <  SRR4302536.534732/94‑1 (MQ=60)
                                                                                             ACCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGACGCCAGACGCTGGATGCGCAG  >  SRR4302536.3193011/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGATGCCTGGCAAATGTTGAGCGCGCCGTTACGCCAGCCGGGCATTGTGGCTTTGCGGGAATATTTACGCCAGCGTCCCCCCGCCTGCATACGGCCTCTTAATCAGGTCGATAACTTATTTATTCTGCCGGTCGCTGAGTGTATTTCGCTCGGTTGGGACAGCAGCCGCCAGACGCTGGATGCGCAG  >  NC_000913/2208412‑2208598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑