Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,209,048 A→G intergenic (+84/‑185) b2122 → / → yehR hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,209,0480AG99.4% 531.0 / ‑5.1 165intergenic (+84/‑185)b2122/yehRhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (74/89);  total (75/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 184 total aligned reads displayed.

GGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGA  >  NC_000913/2208958‑2209140
                                                                                          |                                                                                            
ggCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTTGGACAATTTCGTAATACAGAAAGCGAGGcgcg                                                                                            >  2:1228275/1‑93 (MQ=255)
ggCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCTAGGcgcg                                                                                            >  2:3139819/1‑93 (MQ=255)
ggCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGcgcg                                                                                            >  2:792587/1‑93 (MQ=255)
ggCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGcgcg                                                                                            >  2:1486655/1‑93 (MQ=255)
  cATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCggg                                                                                          >  1:2975636/1‑93 (MQ=255)
    tAGTTGCTGGCGAATGTTCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGGGTTGGGACAAATTCGTAATACAGAAAGCGAGGCGCggggt                                                                                        >  2:4205507/1‑91 (MQ=255)
    tAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCgggga                                                                                        >  1:2823222/1‑91 (MQ=255)
    tAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTa                                                                                        >  2:353449/1‑93 (MQ=255)
    tAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTa                                                                                        <  2:2570031/93‑1 (MQ=255)
    tAGTGGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTa                                                                                        <  1:692228/93‑1 (MQ=255)
     aGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAg                                                                                       >  2:4692711/1‑93 (MQ=255)
      gTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGa                                                                                      <  2:420152/93‑1 (MQ=255)
      gTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGa                                                                                      >  1:2781301/1‑93 (MQ=255)
       ttGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGaa                                                                                     <  1:3221806/93‑1 (MQ=255)
         gCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGc                                                                                   <  1:38495/93‑1 (MQ=255)
          cTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCa                                                                                  >  1:4688525/1‑93 (MQ=255)
           tGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTGATACAGAAAGCGAGGCGCGGGTAGAAGcga                                                                                 <  2:3605854/93‑3 (MQ=255)
           tGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCaa                                                                                 <  1:3904688/93‑1 (MQ=255)
           tGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTAGTAATACAGAAAGCGAGGCGCGGGGAGaaaaca                                                                                 >  2:1835279/1‑89 (MQ=255)
            ggagAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAACAAt                                                                                >  1:1208917/4‑93 (MQ=255)
            ggCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCaaa                                                                                >  1:1937391/1‑92 (MQ=255)
            ggCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAAt                                                                                >  1:3660126/1‑93 (MQ=255)
                aaTGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAAt                                                                            <  2:531875/93‑1 (MQ=255)
                aaTGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAAt                                                                            >  1:3387655/1‑93 (MQ=255)
                aaTGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAAt                                                                            <  2:1930172/93‑1 (MQ=255)
                aaTGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAAt                                                                            <  2:2707139/93‑1 (MQ=255)
                 aTGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATa                                                                           <  1:2154782/93‑1 (MQ=255)
                   gACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAGAGCGAGGCGCGGGTAGAAGCAATGAATAAc                                                                         <  1:113914/93‑1 (MQ=255)
                   gACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAAc                                                                         >  2:2012505/1‑93 (MQ=255)
                    aCCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACg                                                                        <  1:4072232/93‑1 (MQ=255)
                      cTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGgt                                                                      <  1:97129/93‑1 (MQ=255)
                        cACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGgtgt                                                                    <  1:2882750/93‑1 (MQ=255)
                         aCCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGtt                                                                   <  2:4321829/93‑1 (MQ=255)
                         aCCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGGAGAAGCAATGAATAACGGTGtt                                                                   >  1:4270410/1‑93 (MQ=255)
                          ccGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGttt                                                                  >  1:1471533/1‑93 (MQ=255)
                          ccGCGGTGGGTTTGTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGttt                                                                  <  2:3335150/93‑1 (MQ=255)
                           cgcgGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGtttt                                                                 <  1:770803/93‑1 (MQ=255)
                              ggTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGAGAGGCGCGGGTAGAAGGAATGAATAACGGGGTtttgct                                                              >  1:3694564/1‑93 (MQ=255)
                               gTGGGTTTTTATCGGCTGGCACATGTGTTGGGGCAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACCGTGAttttctt                                                             >  2:1331975/1‑93 (MQ=255)
                               gTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTtttgctt                                                             >  1:2640019/1‑93 (MQ=255)
                               gTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTtttgctt                                                             <  1:3943181/93‑1 (MQ=255)
                               gTGGGTTTTTATCGGCTGGCACATGGATTGCGGCAATATCGTAATACAGAAAGCGAGGTGCGGGGAGAAGCAATGAAGTACGGTATtttgatt                                                             >  2:2531481/1‑93 (MQ=255)
                                tGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTtttgcttt                                                            <  1:1810211/93‑1 (MQ=255)
                                  ggTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTtttgctttgc                                                          <  2:1728750/93‑1 (MQ=255)
                                  ggTGTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTtttgctttgc                                                          <  1:3341006/93‑1 (MQ=255)
                                      tttATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAACAACGGTGTTTTGCTTTGCGAAc                                                      <  1:3460288/93‑1 (MQ=255)
                                       ttATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACa                                                     <  1:4364235/93‑1 (MQ=255)
                                          tCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAatt                                                  >  2:2947806/1‑93 (MQ=255)
                                             gCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAattatt                                               >  2:3186840/1‑93 (MQ=255)
                                              cTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTc                                              <  2:4559923/93‑1 (MQ=255)
                                               tGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTcc                                             >  1:3314231/1‑93 (MQ=255)
                                                ggCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCTGGTAGAAGCAATGAATAACGGCGTTTTGCTTTGCGAACAATTATTacc                                            >  1:1196401/1‑93 (MQ=255)
                                                 gCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTcccc                                           >  2:3109765/1‑93 (MQ=255)
                                                 gCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTcccc                                           >  2:2329785/1‑93 (MQ=255)
                                                   acaTGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCAt                                         <  1:2454843/93‑1 (MQ=255)
                                                   acaTGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCAt                                         >  2:2741689/1‑93 (MQ=255)
                                                   acaTGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCAt                                         <  1:3717022/93‑1 (MQ=255)
                                                    caTGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATg                                        >  1:4571333/1‑93 (MQ=255)
                                                    caTGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATg                                        >  1:2278863/1‑93 (MQ=255)
                                                     aTGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGTGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGc                                       >  1:3084175/1‑93 (MQ=255)
                                                     aTGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGc                                       >  2:4739829/1‑93 (MQ=255)
                                                      tgtgTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCt                                      >  2:2333230/1‑93 (MQ=255)
                                                      tgtgTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCt                                      >  1:1330615/1‑93 (MQ=255)
                                                      tgtgTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCt                                      >  1:985482/1‑93 (MQ=255)
                                                       gtgtTGGGACAATTTCGTAATACAGAAAGCGAGGCTCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCtt                                     >  2:1584276/1‑93 (MQ=255)
                                                          ttGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAg                                  >  1:2792254/1‑93 (MQ=255)
                                                           tGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGc                                 <  1:509367/93‑1 (MQ=255)
                                                            gggACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGca                                >  2:2781812/1‑93 (MQ=255)
                                                            gggACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGca                                >  1:4154109/1‑93 (MQ=255)
                                                            gggACAATTTCGTAATACAGAAAGCGAGGCGCGGCTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGca                                <  1:4669807/93‑1 (MQ=255)
                                                             ggACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGcaa                               >  2:3461286/1‑93 (MQ=255)
                                                             ggACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGcaa                               >  1:3414707/1‑93 (MQ=255)
                                                             ggACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGcaa                               <  1:2204894/93‑1 (MQ=255)
                                                             ggACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGcaa                               <  1:2260979/93‑1 (MQ=255)
                                                             ggACAATTTCGGAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGcaa                               >  2:4022678/1‑93 (MQ=255)
                                                                cAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGcaacaa                            <  2:2111440/93‑1 (MQ=255)
                                                                cAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGcaacaa                            <  1:922279/93‑1 (MQ=255)
                                                                 aaTTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTTAGCAACAAg                           >  2:3749866/1‑93 (MQ=255)
                                                                 aaTTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAg                           <  1:2016609/93‑1 (MQ=255)
                                                                   tttCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGa                         <  1:1037046/93‑1 (MQ=255)
                                                                      cGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGa                      <  2:1164537/93‑1 (MQ=255)
                                                                       gTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGaa                     >  2:3909002/1‑93 (MQ=255)
                                                                       gTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGaa                     <  2:619481/93‑1 (MQ=255)
                                                                        tAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGaaa                    <  2:2865210/93‑1 (MQ=255)
                                                                        tAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGaaa                    <  1:825904/93‑1 (MQ=255)
                                                                        tAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAAGATGaaa                    >  1:4153259/1‑93 (MQ=255)
                                                                         aaTACAGAAAGCGAGGCGCGGGTCGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAAt                   <  2:2580974/93‑1 (MQ=255)
                                                                         aaTACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAAt                   >  2:4038632/1‑93 (MQ=255)
                                                                           tACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAg                 >  1:10941/1‑93 (MQ=255)
                                                                            aCAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTACCCATGCTTCAGCAACAAGGATGAAATAGt                >  2:181326/1‑93 (MQ=255)
                                                                               gAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCt             >  2:4099988/1‑93 (MQ=255)
                                                                               gAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCt             <  2:1556623/93‑1 (MQ=255)
                                                                               gAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCt             <  2:671236/93‑1 (MQ=255)
                                                                                aaaGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCtt            <  1:1612354/93‑1 (MQ=255)
                                                                                  aGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCtttt          >  2:1653251/1‑93 (MQ=255)
                                                                                  aGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCtttt          >  2:929858/1‑93 (MQ=255)
                                                                                    cGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTa        >  2:458257/1‑93 (MQ=255)
                                                                                      aGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACt      <  2:4341290/93‑1 (MQ=255)
                                                                                      aGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACt      >  1:4187399/1‑93 (MQ=255)
                                                                                       ggCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTa     >  1:2326111/1‑93 (MQ=255)
                                                                                          |                                                                                            
GGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGA  >  NC_000913/2208958‑2209140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAG  >  NC_000913/2208956‑2209141
                                                                                            |                                                                                             
CAGGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGC                                                                                              >  SRR4302544.2654196/1‑94 (MQ=60)
   GCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGG                                                                                           <  SRR4302544.4200477/94‑1 (MQ=60)
   GCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGG                                                                                           >  SRR4302544.3004180/1‑94 (MQ=60)
     ATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGGA                                                                                         >  SRR4302544.2850503/1‑94 (MQ=60)
     ATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGGGTTGGGACAATTTCGTAATACAGAAAAGGAGGCGCGGGTA                                                                                         >  SRR4302544.3888632/1‑94 (MQ=60)
      TAGTGGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAG                                                                                        <  SRR4302544.699126/94‑1 (MQ=60)
       AGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGA                                                                                       >  SRR4302544.2808073/1‑94 (MQ=60)
         TTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAG                                                                                     <  SRR4302544.3252561/94‑1 (MQ=60)
          TGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGcaga                                                                                    >  SRR4302544.1666262/1‑90 (MQ=60)
           GCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCA                                                                                   <  SRR4302544.38875/94‑1 (MQ=60)
           GCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCA                                                                                   >  SRR4302544.4734857/1‑94 (MQ=60)
             TGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAAT                                                                                 <  SRR4302544.3943482/94‑1 (MQ=60)
             TGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAAT                                                                                 >  SRR4302544.3696640/1‑94 (MQ=60)
             TGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAAA                                                                                 >  SRR4302544.1956146/1‑94 (MQ=60)
             TGGAGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAACAAT                                                                                 >  SRR4302544.1220891/1‑94 (MQ=60)
                CGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAA                                                                              <  SRR4302544.3582347/94‑1 (MQ=60)
                CGAATGACCTCACCGCGGCGGGTTTTTATCGGCTGGCACACGTGTTGGGACAATTTCGTAATAAAGAAAGCGAGGCGCGGGGAGAACGAATGAA                                                                              >  SRR4302544.80168/1‑94 (MQ=60)
                CGAATGACCTCACCGCGGCGGGTTTTTATCGGCTGGCAACTGTGTTGGGGCAATTTCGTAAAACAGAAAGCGAGGCGCGGGTAGAAGAAAAGAA                                                                              >  SRR4302544.80167/1‑94 (MQ=60)
                 GAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGCGGCGCGGGTAGAAGCAATGAAA                                                                             >  SRR4302544.713621/1‑94 (MQ=60)
                 GAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAAT                                                                             <  SRR4302544.3729305/94‑1 (MQ=60)
                 GAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAAT                                                                             >  SRR4302544.3420029/1‑94 (MQ=60)
                 GAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAAT                                                                             >  SRR4302544.4180424/1‑94 (MQ=60)
                 GAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAAA                                                                             >  SRR4302544.3420028/1‑94 (MQ=60)
                 GAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAAA                                                                             >  SRR4302544.130999/1‑94 (MQ=60)
                 GAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGGGTTGGGACAATTTCGTAATACAGAAAGCGGGGCGCGGGGAGAAGCAATGAAT                                                                             >  SRR4302544.1167271/1‑94 (MQ=60)
                   ATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAA                                                                           <  SRR4302544.2175270/94‑1 (MQ=60)
                    TGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAAC                                                                          >  SRR4302544.195243/1‑94 (MQ=60)
                     GACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAGAGCGAGGCGCGGGTAGAAGCAATGAATAACG                                                                         <  SRR4302544.114952/94‑1 (MQ=60)
                     GACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACG                                                                         <  SRR4302544.114951/94‑1 (MQ=60)
                      ACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGG                                                                        <  SRR4302544.4112758/94‑1 (MQ=60)
                       CCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGG                                                                       >  SRR4302544.3441667/1‑94 (MQ=60)
                        CTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTG                                                                      <  SRR4302544.98016/94‑1 (MQ=60)
                        CTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTG                                                                      >  SRR4302544.4297841/1‑94 (MQ=60)
                        CTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTG                                                                      <  SRR4302544.2488419/94‑1 (MQ=60)
                        CTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGGG                                                                      >  SRR4302544.3949559/1‑94 (MQ=60)
                          CACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTT                                                                    <  SRR4302544.2910559/94‑1 (MQ=60)
                          CACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGGAGAAGCAATGAATAACGGTGTT                                                                    >  SRR4302544.4313995/1‑94 (MQ=60)
                           ACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTT                                                                   >  SRR4302544.1486106/1‑94 (MQ=60)
                           ACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTT                                                                   <  SRR4302544.84098/94‑1 (MQ=60)
                             CGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTG                                                                 <  SRR4302544.778304/94‑1 (MQ=60)
                               CGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCT                                                               >  SRR4302544.2807974/1‑94 (MQ=60)
                               CGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGAGAGGCGCGGGTAGAAGGAATGAATAACGGGGTTTTGCT                                                               >  SRR4302544.3731369/1‑94 (MQ=60)
                                GGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTT                                                              >  SRR4302544.2665325/1‑94 (MQ=60)
                                GGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTT                                                              >  SRR4302544.2829799/1‑94 (MQ=60)
                                 GTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTT                                                             <  SRR4302544.3982360/94‑1 (MQ=60)
                                  TGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTG                                                            <  SRR4302544.1827892/94‑1 (MQ=60)
                                   GGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGC                                                           <  SRR4302544.3171491/94‑1 (MQ=60)
                                    GGTGTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCG                                                          <  SRR4302544.3372858/94‑1 (MQ=60)
                                     GTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGA                                                         >  SRR4302544.2943109/1‑94 (MQ=60)
                                        TTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAACAACGGTGTTTTGCTTTGCGAACA                                                      <  SRR4302544.3493312/94‑1 (MQ=60)
                                         TTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAA                                                     <  SRR4302544.4408510/94‑1 (MQ=60)
                                              GGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATT                                                >  SRR4302544.4413113/1‑94 (MQ=60)
                                              GGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATT                                                <  SRR4302544.4736784/94‑1 (MQ=60)
                                               GCTGGCACATGTGTTGGGACAATTGCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTC                                               <  SRR4302544.121003/94‑1 (MQ=60)
                                                CTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCC                                              >  SRR4302544.3345793/1‑94 (MQ=60)
                                                 TGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCTGGTAGAAGCAATGAATAACGGCGTTTTGCTTTGCGAACAATTATTACC                                             >  SRR4302544.1208260/1‑94 (MQ=60)
                                                 TGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCC                                             <  SRR4302544.4038709/94‑1 (MQ=60)
                                                    CACATGTGTTGGGACAATTTCGTAATACAGACAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAAACATTATTCCACAT                                          >  SRR4302544.2287732/1‑94 (MQ=60)
                                                     ACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATG                                         >  SRR4302544.4616896/1‑94 (MQ=60)
                                                     ACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATG                                         >  SRR4302544.2300325/1‑94 (MQ=60)
                                                     ACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATG                                         <  SRR4302544.3753999/94‑1 (MQ=60)
                                                     ACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATG                                         <  SRR4302544.3770873/94‑1 (MQ=60)
                                                     ACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATG                                         >  SRR4302544.2910430/1‑94 (MQ=60)
                                                     ACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATG                                         <  SRR4302544.2478261/94‑1 (MQ=60)
                                                      CATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGTGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGC                                        >  SRR4302544.3113556/1‑94 (MQ=60)
                                                       ATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCT                                       >  SRR4302544.995145/1‑94 (MQ=60)
                                                       ATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCT                                       >  SRR4302544.1343708/1‑94 (MQ=60)
                                                        TGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTT                                      >  SRR4302544.2330902/1‑94 (MQ=60)
                                                          TGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCA                                    <  SRR4302544.3331165/94‑1 (MQ=60)
                                                          TGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCA                                    >  SRR4302544.201303/1‑94 (MQ=60)
                                                           GTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAG                                   <  SRR4302544.1476768/94‑1 (MQ=60)
                                                           GTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAG                                   >  SRR4302544.2819141/1‑94 (MQ=60)
                                                             TGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCA                                 >  SRR4302544.4195252/1‑94 (MQ=60)
                                                             TGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCA                                 <  SRR4302544.514394/94‑1 (MQ=60)
                                                              GGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAA                                >  SRR4302544.3447343/1‑94 (MQ=60)
                                                              GGGACAATTTCGTAATACAGAAAGCGAGGCGCGGCTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAA                                <  SRR4302544.4715969/94‑1 (MQ=60)
                                                               GGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAAC                               <  SRR4302544.2225658/94‑1 (MQ=60)
                                                               GGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAAC                               <  SRR4302544.2282250/94‑1 (MQ=60)
                                                                 ACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAA                             <  SRR4302544.632460/94‑1 (MQ=60)
                                                                  CAATTTCGTAATACGGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAG                            <  SRR4302544.931372/94‑1 (MQ=60)
                                                                  CAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAG                            <  SRR4302544.931371/94‑1 (MQ=60)
                                                                  CAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAG                            <  SRR4302544.3099007/94‑1 (MQ=60)
                                                                   AATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGG                           <  SRR4302544.2036196/94‑1 (MQ=60)
                                                                     TTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGAT                         <  SRR4302544.1047357/94‑1 (MQ=60)
                                                                         GTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAAGATGAAA                     >  SRR4302544.4194392/1‑94 (MQ=60)
                                                                          TAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAAT                    <  SRR4302544.833999/94‑1 (MQ=60)
                                                                           AATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATA                   >  SRR4302544.2153272/1‑94 (MQ=60)
                                                                           AATACAGAAAGCGAGGCGCGGGGAGAAGCAATGAATAACGGTGTTTTGCTTTGCTAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATA                   <  SRR4302544.347773/94‑1 (MQ=60)
                                                                            ATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAG                  >  SRR4302544.11040/1‑94 (MQ=60)
                                                                                  AAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTT            <  SRR4302544.1628250/94‑1 (MQ=60)
                                                                                     GCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTA         <  SRR4302544.465613/94‑1 (MQ=60)
                                                                                      CGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTAC        <  SRR4302544.2415471/94‑1 (MQ=60)
                                                                                       GAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACT       >  SRR4302544.4228871/1‑94 (MQ=60)
                                                                                        AGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTA      >  SRR4302544.2348164/1‑94 (MQ=60)
                                                                                            GCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAG  <  SRR4302544.2574457/94‑1 (MQ=60)
                                                                                            ACGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAG  <  SRR4302544.2181843/94‑1 (MQ=60)
                                                                                            |                                                                                             
CAGGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAG  >  NC_000913/2208956‑2209141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑