Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,209,048 A→G intergenic (+84/‑185) b2122 → / → yehR hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,209,0480AG99.6% 709.2 / ‑4.8 220intergenic (+84/‑185)b2122/yehRhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (90/129);  total (91/129)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

Only 100 of 236 total aligned reads displayed.

GGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTA  >  NC_000913/2208958‑2209137
                                                                                          |                                                                                         
ggCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGcgcg                                                                                         >  1:616566/1‑93 (MQ=255)
ggCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGcgcg                                                                                         >  1:4327166/1‑93 (MQ=255)
 gCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCgg                                                                                        <  2:5528048/93‑1 (MQ=255)
   aTAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCgggg                                                                                      >  1:4771620/1‑92 (MQ=255)
   aTAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCggga                                                                                      >  2:6435666/1‑92 (MQ=255)
   aTAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGt                                                                                      >  1:3183358/1‑93 (MQ=255)
   aTAGTTGCTGGCGAATGACCTCACCGCGGTGGGGTTTTATCGGCTGGCACATGTGTTGGGGCAATTTCGTAATACAGAAAACGAGGCGCgggg                                                                                      >  2:5864699/1‑92 (MQ=255)
    tAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCgggga                                                                                     >  1:1556823/1‑91 (MQ=255)
    tAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTa                                                                                     >  1:1824690/1‑93 (MQ=255)
    tAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTa                                                                                     <  2:6336927/93‑1 (MQ=255)
    tAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAAATTCGTAATACAGAAAGCGAGGCGCGGGTa                                                                                     >  2:751809/1‑93 (MQ=255)
       ttGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACCTGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGaa                                                                                  >  1:3305782/1‑93 (MQ=255)
       ttGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGaa                                                                                  <  2:4294688/93‑1 (MQ=255)
        tGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAg                                                                                 <  1:1190223/93‑1 (MQ=255)
        tGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAg                                                                                 >  2:297684/1‑93 (MQ=255)
         gCTGGCGAATGACCTCACCGCGGTGGGGTTTTATCGGCTGGGACATGTGTTGGGACAAATTCGTAATACAGAAAGCGAGGCGCGGGTTGAAga                                                                                >  1:6094853/1‑92 (MQ=255)
           tGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCaa                                                                              <  1:2834241/93‑1 (MQ=255)
            ggCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAAt                                                                             >  1:1519324/1‑93 (MQ=255)
            ggCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAAt                                                                             <  1:509925/93‑1 (MQ=255)
            ggCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAAt                                                                             <  2:5319125/93‑1 (MQ=255)
              cGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGa                                                                           <  1:5117498/93‑1 (MQ=255)
               gAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGaa                                                                          <  1:3219513/93‑1 (MQ=255)
                aaTGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAAt                                                                         <  1:668899/93‑1 (MQ=255)
                 aTGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATa                                                                        <  1:5062391/93‑1 (MQ=255)
                  tGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATaa                                                                       >  1:650874/1‑93 (MQ=255)
                   gACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAAc                                                                      <  1:3473978/93‑1 (MQ=255)
                   gACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAAc                                                                      <  1:6268191/93‑1 (MQ=255)
                     ccTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACgg                                                                    <  1:605280/93‑1 (MQ=255)
                     ccTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACgg                                                                    <  2:5070394/93‑1 (MQ=255)
                     ccTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACgg                                                                    <  2:2000444/93‑1 (MQ=255)
                     ccTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACgg                                                                    <  1:3645771/93‑1 (MQ=255)
                      cTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACggg                                                                   >  2:1934832/1‑92 (MQ=255)
                      cTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGgt                                                                   <  1:2262401/93‑1 (MQ=255)
                       tCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGgtg                                                                  >  2:5496724/1‑93 (MQ=255)
                          ccGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGttt                                                               <  1:5619399/93‑1 (MQ=255)
                           cgcgGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGtttt                                                              <  2:1069022/93‑1 (MQ=255)
                            gcgGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTtttg                                                             >  1:1659653/1‑93 (MQ=255)
                            gcgGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTtttg                                                             >  2:1124946/1‑93 (MQ=255)
                            gcgGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGGGTtttg                                                             >  1:855768/1‑93 (MQ=255)
                             cgGTGGTTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTtttgc                                                            <  1:5679947/93‑1 (MQ=255)
                             cgGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTtttgc                                                            >  2:408693/1‑93 (MQ=255)
                              ggTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTtttgct                                                           <  1:5918009/93‑1 (MQ=255)
                               gTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTtttgctt                                                          >  2:124364/1‑93 (MQ=255)
                               gTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTtttgctt                                                          >  2:2998968/1‑93 (MQ=255)
                               gTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTtttgctt                                                          <  1:4526511/93‑1 (MQ=255)
                                tGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTtttgcttt                                                         >  1:5416264/1‑93 (MQ=255)
                                 gggTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTtttgctttg                                                        <  2:6455926/93‑1 (MQ=255)
                                   gTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCg                                                      <  1:3423654/93‑1 (MQ=255)
                                    tttttATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGa                                                     <  1:88868/93‑1 (MQ=255)
                                       ttATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACa                                                  <  1:411910/93‑1 (MQ=255)
                                          tCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAatt                                               >  1:6599640/1‑93 (MQ=255)
                                          tCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAatt                                               <  2:5578134/93‑1 (MQ=255)
                                            ggCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAattat                                             <  2:3102181/93‑1 (MQ=255)
                                               tGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTcc                                          <  2:1750963/93‑1 (MQ=255)
                                                ggCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTccc                                         >  2:3706144/1‑93 (MQ=255)
                                                  cacaTGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCa                                       <  2:794687/93‑1 (MQ=255)
                                                  cacaTGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCa                                       >  2:2372248/1‑93 (MQ=255)
                                                   acaTGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCAt                                      >  1:2765275/1‑93 (MQ=255)
                                                       gtgtTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCtt                                  <  2:3242575/93‑1 (MQ=255)
                                                        tgtTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTc                                 <  2:5257281/93‑1 (MQ=255)
                                                         gtTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCa                                <  2:4080119/93‑1 (MQ=255)
                                                         gtTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCa                                >  1:3979078/1‑93 (MQ=255)
                                                         gtTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCa                                >  1:4430913/1‑93 (MQ=255)
                                                           tGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGc                              <  2:6043022/93‑1 (MQ=255)
                                                            gggACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGca                             >  1:3086476/1‑93 (MQ=255)
                                                            gggACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGca                             >  1:4749727/1‑93 (MQ=255)
                                                               aCAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGcaaca                          >  1:5778203/1‑93 (MQ=255)
                                                                cAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGcaacaa                         <  1:6684675/93‑1 (MQ=255)
                                                                 aaTTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAGTAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAg                        >  2:3561177/1‑93 (MQ=255)
                                                                 aaTTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAg                        <  2:4810773/93‑1 (MQ=255)
                                                                 aaTTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAg                        <  2:5693575/93‑1 (MQ=255)
                                                                   tttCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGa                      <  2:1630375/93‑1 (MQ=255)
                                                                   tttCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGa                      <  1:3389803/93‑1 (MQ=255)
                                                                    ttCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGAt                     <  1:1158305/93‑1 (MQ=255)
                                                                     tCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATg                    <  1:3877302/93‑1 (MQ=255)
                                                                     tCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATg                    >  2:5944671/1‑93 (MQ=255)
                                                                     tCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATg                    <  1:1997683/93‑1 (MQ=255)
                                                                      cGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGa                   <  2:2711527/93‑1 (MQ=255)
                                                                       gTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGaa                  >  1:2845497/1‑93 (MQ=255)
                                                                       gTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGaa                  <  1:5299564/93‑1 (MQ=255)
                                                                        tAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGaaa                 <  2:6195899/93‑1 (MQ=255)
                                                                        tAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGaaa                 <  1:4275858/93‑1 (MQ=255)
                                                                        tAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGaaa                 >  2:4866008/1‑93 (MQ=255)
                                                                        tAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGaaa                 <  1:4884962/93‑1 (MQ=255)
                                                                          aTACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATa               >  2:2503405/1‑93 (MQ=255)
                                                                          aTACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATa               <  1:2518845/93‑1 (MQ=255)
                                                                            aCAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGt             <  1:4249307/93‑1 (MQ=255)
                                                                             cAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTg            >  2:611579/1‑93 (MQ=255)
                                                                             cAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTg            <  2:935993/93‑1 (MQ=255)
                                                                              aGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGc           <  2:1511861/93‑1 (MQ=255)
                                                                               gAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCt          <  1:4579785/93‑1 (MQ=255)
                                                                               gAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCt          <  1:6335567/93‑1 (MQ=255)
                                                                               gAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCt          >  1:97177/1‑93 (MQ=255)
                                                                                aaaGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCtt         <  2:4653814/93‑1 (MQ=255)
                                                                                   gCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCttttt      >  1:2690096/1‑93 (MQ=255)
                                                                                    cGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTa     <  2:343492/93‑1 (MQ=255)
                                                                                     gAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTAc    >  1:6725693/1‑93 (MQ=255)
                                                                                      aGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACt   >  2:5773882/1‑93 (MQ=255)
                                                                                      aGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACt   <  2:3917141/93‑1 (MQ=255)
                                                                                       ggCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTa  <  1:136522/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTA  >  NC_000913/2208958‑2209137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAG  >  NC_000913/2208956‑2209141
                                                                                            |                                                                                             
CAGGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACCATTTCGTAATACAGAAAGCGAGGCGC                                                                                              >  SRR4302536.2589739/1‑94 (MQ=60)
CAGGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGC                                                                                              >  SRR4302536.3028883/1‑94 (MQ=60)
 AGGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCG                                                                                             <  SRR4302536.4766257/94‑1 (MQ=60)
 AGGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCG                                                                                             >  SRR4302536.4456739/1‑94 (MQ=60)
 AGGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCG                                                                                             >  SRR4302536.634925/1‑94 (MQ=60)
 AGGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAACGAGGCGCG                                                                                             >  SRR4302536.6899455/1‑94 (MQ=60)
   GCATAGTTGCTGGCGCATCACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGG                                                                                           <  SRR4302536.3055735/94‑1 (MQ=60)
   GCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGG                                                                                           <  SRR4302536.1308420/94‑1 (MQ=60)
    CATAGTTGCTGGCGAATGACCTCACCGCGTTAGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTGCGTAATACAGAAAGCGAGGCGCGGGT                                                                                          <  SRR4302536.6425848/94‑1 (MQ=60)
    CATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGT                                                                                          >  SRR4302536.3276702/1‑94 (MQ=60)
    CATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGG                                                                                          >  SRR4302536.4915310/1‑94 (MQ=60)
     ATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGGCAATTTCGTAATACAGAAAGCGAGGCGCGGGTA                                                                                         >  SRR4302536.1198233/1‑94 (MQ=60)
     ATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTA                                                                                         >  SRR4302536.1879729/1‑94 (MQ=60)
     ATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGGA                                                                                         >  SRR4302536.335823/1‑94 (MQ=60)
     ATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGGA                                                                                         >  SRR4302536.1602545/1‑94 (MQ=60)
      TAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAG                                                                                        <  SRR4302536.3714223/94‑1 (MQ=60)
      TAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGGAG                                                                                        >  SRR4302536.6835036/1‑94 (MQ=60)
        GTTGCTGGCGAATGACCTCAGCACGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAAAAAGCGAGGCGCGGGTAGAA                                                                                      <  SRR4302536.3327160/94‑1 (MQ=60)
        GTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACCTGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAA                                                                                      >  SRR4302536.3403366/1‑94 (MQ=60)
        GTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAA                                                                                      <  SRR4302536.5340705/94‑1 (MQ=60)
          TGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGC                                                                                    <  SRR4302536.1226020/94‑1 (MQ=60)
          TGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGGAGAAGC                                                                                    >  SRR4302536.6108222/1‑94 (MQ=60)
          TGCTGGCGAATGACCTCACCGCGGTGGGGTTTTATCGGCTGGGACATGTGTTGGGACAAATTCGTAATACAGAAAGCGAGGCGCGGGTTGAAGA                                                                                    >  SRR4302536.6276585/1‑94 (MQ=60)
             TGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAAT                                                                                 <  SRR4302536.2918127/94‑1 (MQ=60)
             TGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGGAGAAGCAAA                                                                                 >  SRR4302536.1564048/1‑94 (MQ=60)
              GGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATG                                                                                <  SRR4302536.523000/94‑1 (MQ=60)
              GGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATG                                                                                <  SRR4302536.5071990/94‑1 (MQ=60)
               GCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGA                                                                               <  SRR4302536.2981544/94‑1 (MQ=60)
                CGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAA                                                                              <  SRR4302536.5270421/94‑1 (MQ=60)
                 GAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGGAATGAAT                                                                             >  SRR4302536.6068410/1‑94 (MQ=60)
                 GAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAAT                                                                             <  SRR4302536.3313923/94‑1 (MQ=60)
                  AATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATA                                                                            <  SRR4302536.688511/94‑1 (MQ=60)
                   ATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAA                                                                           >  SRR4302536.669976/1‑94 (MQ=60)
                   ATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAA                                                                           <  SRR4302536.5214023/94‑1 (MQ=60)
                    TGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAAC                                                                          >  SRR4302536.745862/1‑94 (MQ=60)
                     TACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACG                                                                         <  SRR4302536.5790302/94‑1 (MQ=60)
                     GACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACG                                                                         <  SRR4302536.407169/94‑1 (MQ=60)
                     GACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACG                                                                         <  SRR4302536.3577529/94‑1 (MQ=60)
                     GACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACG                                                                         <  SRR4302536.6454942/94‑1 (MQ=60)
                      ACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGG                                                                        >  SRR4302536.6278499/1‑94 (MQ=60)
                       CCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGT                                                                       <  SRR4302536.623267/94‑1 (MQ=60)
                       CCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGT                                                                       <  SRR4302536.2438557/94‑1 (MQ=60)
                       CCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGT                                                                       <  SRR4302536.3754765/94‑1 (MQ=60)
                        CTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTG                                                                      <  SRR4302536.2330925/94‑1 (MQ=60)
                          CACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCTGGTAGAAGCAATGAATAACGGTGTT                                                                    <  SRR4302536.6658459/94‑1 (MQ=60)
                           CCCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTT                                                                   <  SRR4302536.516621/94‑1 (MQ=60)
                            gggggttttttttggtgggcggggttgttgtgtgaaaataattaaaaaaaaaaaaagGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTT                                                                  <  SRR4302536.3930597/37‑1 (MQ=60)
                            CCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTT                                                                  <  SRR4302536.5786315/94‑1 (MQ=60)
                             CGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTG                                                                 <  SRR4302536.5268728/94‑1 (MQ=60)
                             CGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTG                                                                 >  SRR4302536.1707833/1‑94 (MQ=60)
                             CGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGGGTTTTG                                                                 >  SRR4302536.880928/1‑94 (MQ=60)
                              GCGGTGGGTTTTTATCGGCTGGCCCATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGC                                                                >  SRR4302536.4513557/1‑94 (MQ=60)
                              GCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGACGCAATGAATAACGGgtgtttgt                                                                >  SRR4302536.4371404/1‑86 (MQ=60)
                               CGGTGGTTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCG                                                               <  SRR4302536.5848819/94‑1 (MQ=60)
                                GGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTT                                                              <  SRR4302536.6094286/94‑1 (MQ=60)
                                 GTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTT                                                             >  SRR4302536.5578142/1‑94 (MQ=60)
                                 GTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTT                                                             <  SRR4302536.4662234/94‑1 (MQ=60)
                                 GTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTT                                                             <  SRR4302536.4093753/94‑1 (MQ=60)
                                  TGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTG                                                            <  SRR4302536.6290915/94‑1 (MQ=60)
                                   GGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGC                                                           <  SRR4302536.1590922/94‑1 (MQ=60)
                                    GGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCG                                                          <  SRR4302536.1021078/94‑1 (MQ=60)
                                    GGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCG                                                          >  SRR4302536.2030364/1‑94 (MQ=60)
                                     GTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGA                                                         <  SRR4302536.3525555/94‑1 (MQ=60)
                                      TTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAA                                                        <  SRR4302536.91244/94‑1 (MQ=60)
                                        TTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACA                                                      <  SRR4302536.768041/94‑1 (MQ=60)
                                         TTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAA                                                     <  SRR4302536.422776/94‑1 (MQ=60)
                                           ATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATT                                                   >  SRR4302536.6795933/1‑94 (MQ=60)
                                             CGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTAT                                                 <  SRR4302536.947140/94‑1 (MQ=60)
                                                 TGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCC                                             <  SRR4302536.4394493/94‑1 (MQ=60)
                                                 TGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCC                                             >  SRR4302536.3543369/1‑94 (MQ=60)
                                                 TGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCC                                             >  SRR4302536.5617530/1‑94 (MQ=60)
                                                   GCACATGTGTTGGGACAAGTTCGTCATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTATTCGAACAATTATTCCCCG                                           >  SRR4302536.4822580/1‑94 (MQ=60)
                                                    CACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCAT                                          <  SRR4302536.101223/94‑1 (MQ=60)
                                                    CACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCAT                                          >  SRR4302536.2847513/1‑94 (MQ=60)
                                                        TGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTT                                      >  SRR4302536.1497045/1‑94 (MQ=60)
                                                        TGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGCGCTTTGGTTTGTGATCAATTATTCCCCATACTT                                      >  SRR4302536.4945054/1‑94 (MQ=60)
                                                          TGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCA                                    >  SRR4302536.4097208/1‑94 (MQ=60)
                                                          TGTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCA                                    >  SRR4302536.4563753/1‑94 (MQ=60)
                                                           GTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAG                                   <  SRR4302536.4236099/94‑1 (MQ=60)
                                                           GTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAG                                   >  SRR4302536.1776690/1‑94 (MQ=60)
                                                           GTTGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAG                                   >  SRR4302536.2674203/1‑94 (MQ=60)
                                                             TGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCA                                 <  SRR4302536.3493222/94‑1 (MQ=60)
                                                             TGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCA                                 >  SRR4302536.3176748/1‑94 (MQ=60)
                                                             TGGGACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCA                                 >  SRR4302536.4892672/1‑94 (MQ=60)
                                                              GGGACAATTTCGTAATACAGATAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAA                                >  SRR4302536.2885654/1‑94 (MQ=60)
                                                              GGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAA                                >  SRR4302536.5221587/1‑94 (MQ=60)
                                                                GACAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACA                              >  SRR4302536.5950147/1‑94 (MQ=60)
                                                                  CAATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAG                            <  SRR4302536.6883217/94‑1 (MQ=60)
                                                                   AATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGG                           <  SRR4302536.2785038/94‑1 (MQ=60)
                                                                   AATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGG                           >  SRR4302536.3824453/1‑94 (MQ=60)
                                                                   AATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGG                           >  SRR4302536.6885858/1‑94 (MQ=60)
                                                                   AATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGG                           <  SRR4302536.4701234/94‑1 (MQ=60)
                                                                   AATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGG                           >  SRR4302536.340910/1‑94 (MQ=60)
                                                                    ATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGA                          >  SRR4302536.1658777/1‑94 (MQ=60)
                                                                    ATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGA                          <  SRR4302536.6790731/94‑1 (MQ=60)
                                                                    ATTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGA                          >  SRR4302536.5375308/1‑94 (MQ=60)
                                                                     TTTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGAT                         <  SRR4302536.3490625/94‑1 (MQ=60)
                                                                      TTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATG                        <  SRR4302536.140141/94‑1 (MQ=60)
                                                                      TTCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATG                        <  SRR4302536.1193172/94‑1 (MQ=60)
                                                                       TCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGA                       <  SRR4302536.329985/94‑1 (MQ=60)
                                                                       TCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGA                       <  SRR4302536.4611615/94‑1 (MQ=60)
                                                                       TCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGA                       <  SRR4302536.2057798/94‑1 (MQ=60)
                                                                       TCGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGA                       <  SRR4302536.3992664/94‑1 (MQ=60)
                                                                        CGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAA                      >  SRR4302536.3237164/1‑94 (MQ=60)
                                                                        CGTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAA                      >  SRR4302536.2929649/1‑94 (MQ=60)
                                                                         GTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAA                     >  SRR4302536.5468421/1‑94 (MQ=60)
                                                                         GTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAA                     <  SRR4302536.5457613/94‑1 (MQ=60)
                                                                         GTAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAA                     >  SRR4302536.6273739/1‑94 (MQ=60)
                                                                          TAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAAT                    <  SRR4302536.4403312/94‑1 (MQ=60)
                                                                          TAATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAAT                    <  SRR4302536.5031909/94‑1 (MQ=60)
                                                                            ATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAG                  <  SRR4302536.3531892/94‑1 (MQ=60)
                                                                            ATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAG                  <  SRR4302536.2594786/94‑1 (MQ=60)
                                                                            ATACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAG                  <  SRR4302536.6034296/94‑1 (MQ=60)
                                                                              ACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTG                <  SRR4302536.4375882/94‑1 (MQ=60)
                                                                              ACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTG                <  SRR4302536.5898344/94‑1 (MQ=60)
                                                                              ACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTG                >  SRR4302536.90031/1‑94 (MQ=60)
                                                                              ACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTG                <  SRR4302536.3370719/94‑1 (MQ=60)
                                                                              ACAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTG                >  SRR4302536.4416288/1‑94 (MQ=60)
                                                                               CAGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGC               >  SRR4302536.187195/1‑94 (MQ=60)
                                                                                AGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCT              >  SRR4302536.4477758/1‑94 (MQ=60)
                                                                                AGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCT              >  SRR4302536.99758/1‑94 (MQ=60)
                                                                                AGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCT              >  SRR4302536.2199698/1‑94 (MQ=60)
                                                                                AGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCT              >  SRR4302536.487342/1‑94 (MQ=60)
                                                                                AGAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCT              <  SRR4302536.2881364/94‑1 (MQ=60)
                                                                                 GAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTT             <  SRR4302536.6524248/94‑1 (MQ=60)
                                                                                 GAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTT             <  SRR4302536.2333937/94‑1 (MQ=60)
                                                                                 GAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTT             >  SRR4302536.6844075/1‑94 (MQ=60)
                                                                                 GAAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTT             <  SRR4302536.4717261/94‑1 (MQ=60)
                                                                                  AAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTT            >  SRR4302536.555522/1‑94 (MQ=60)
                                                                                  AAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTT            >  SRR4302536.4783813/1‑94 (MQ=60)
                                                                                  AAAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATAAAATAGTGCTTT            >  SRR4302536.4276614/1‑94 (MQ=60)
                                                                                   AAGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTT           <  SRR4302536.2922466/94‑1 (MQ=60)
                                                                                    AGCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTT          >  SRR4302536.2770409/1‑94 (MQ=60)
                                                                                     GCGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTCGCGAACAATTATTCCCCATGCTTCAGCACCAAGGATGAAATAGTGCTTTTTA         <  SRR4302536.654828/94‑1 (MQ=60)
                                                                                      CGAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTAC        >  SRR4302536.6925476/1‑94 (MQ=60)
                                                                                       GAGGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACT       >  SRR4302536.6506711/1‑94 (MQ=60)
                                                                                         GGCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAA     <  SRR4302536.140090/94‑1 (MQ=60)
                                                                                          GCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAG    <  SRR4302536.4508995/94‑1 (MQ=60)
                                                                                          GCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAG    <  SRR4302536.1227406/94‑1 (MQ=60)
                                                                                          GCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAG    <  SRR4302536.6271789/94‑1 (MQ=60)
                                                                                          GCGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATAAAATAGTGCTTTTTACTAAG    <  SRR4302536.6720374/94‑1 (MQ=60)
                                                                                           CGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGA   >  SRR4302536.747300/1‑94 (MQ=60)
                                                                                           CGCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGA   <  SRR4302536.3800066/94‑1 (MQ=60)
                                                                                            GCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCTCATGCTTCTGCAACAAGGATGAAATAGTGCTTCTTACTAAGAG  >  SRR4302536.6923005/1‑94 (MQ=60)
                                                                                            GCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAG  >  SRR4302536.2442448/1‑94 (MQ=60)
                                                                                            GCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAG  >  SRR4302536.3177079/1‑94 (MQ=60)
                                                                                            GCGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAG  <  SRR4302536.5173195/94‑1 (MQ=60)
                                                                                            |                                                                                             
CAGGCATAGTTGCTGGCGAATGACCTCACCGCGGTGGGTTTTTATCGGCTGGCACATGTGTTGGGACAATTTCGTAATACAGAAAGCGAGGCACGGGTAGAAGCAATGAATAACGGTGTTTTGCTTTGCGAACAATTATTCCCCATGCTTCAGCAACAAGGATGAAATAGTGCTTTTTACTAAGAG  >  NC_000913/2208956‑2209141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑