Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,246,351	T→C	S68G (AGT→GGT)	lysP ←	lysine‑specific permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,246,351	0	T	C	97.3%	457.7 / 0.4	149	S68G (AGT→GGT)	lysP	lysine‑specific permease
Reads supporting (aligned to +/- strand): ref base T (2/2); new base C (88/57); total (90/59)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 152 total aligned reads displayed.

GCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGG  >  NC_000913/2246261‑2246440

gcgcGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCgg                                                                                         >  2:4111180/1‑93 (MQ=255)

     aaGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGtcatc                                                                                    >  2:4020337/1‑93 (MQ=255)

     aaGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGtcatc                                                                                    >  1:2815254/1‑93 (MQ=255)

     aaGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGtcatc                                                                                    >  1:1196744/1‑93 (MQ=255)

     aaGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGtcatc                                                                                    <  1:4540850/93‑1 (MQ=255)

      aGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGtcatca                                                                                   >  2:1722767/1‑93 (MQ=255)

        ccAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAgg                                                                                 >  2:3743048/1‑93 (MQ=255)

        ccAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAgg                                                                                 <  1:3928308/93‑1 (MQ=255)

        ccAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAgg                                                                                 >  1:1306236/1‑93 (MQ=255)

         cAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGa                                                                                >  1:4238411/1‑93 (MQ=255)

         cAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGa                                                                                >  2:781000/1‑93 (MQ=255)

           aaGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAg                                                                              >  2:3477026/1‑93 (MQ=255)

            aGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGt                                                                             <  1:1086990/93‑1 (MQ=255)

            aGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGt                                                                             >  1:1773627/1‑93 (MQ=255)

             gCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTa                                                                            >  2:2427677/1‑93 (MQ=255)

             gCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTa                                                                            >  2:1545327/1‑93 (MQ=255)

              ccTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTAACCGAGACCGGTCATCAGGAAGTaa                                                                           >  2:3881195/1‑93 (MQ=255)

                ttcttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAAc                                                                         <  1:1882413/93‑1 (MQ=255)

                ttcttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAAc                                                                         >  2:1859947/1‑93 (MQ=255)

                ttcttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAAc                                                                         >  2:621493/1‑93 (MQ=255)

                   ttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCAt                                                                      <  2:3779305/93‑1 (MQ=255)

                    tcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATc                                                                     <  1:2378064/93‑1 (MQ=255)

                      aaCATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAg                                                                   <  2:4223357/93‑1 (MQ=255)

                       aCATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCTAGACCGGTCATCAGGAAGTAAACCATCAgg                                                                  >  2:4742461/1‑93 (MQ=255)

                        cATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGc                                                                 <  1:586157/93‑1 (MQ=255)

                        cATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGc                                                                 <  1:3512537/93‑1 (MQ=255)

                        cATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGc                                                                 <  1:1811509/93‑1 (MQ=255)

                          tAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCa                                                               >  1:3507441/1‑93 (MQ=255)

                            gTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAAt                                                             >  2:134536/1‑93 (MQ=255)

                            gTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAAt                                                             >  2:3012385/1‑93 (MQ=255)

                             ttCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGTAAGTAAACCATCAGGCCAATc                                                            <  1:549065/93‑1 (MQ=255)

                              tCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCa                                                           <  2:830263/93‑1 (MQ=255)

                              tCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCa                                                           <  2:2994977/93‑1 (MQ=255)

                              tCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCa                                                           <  1:1514257/93‑1 (MQ=255)

                               cTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAg                                                          <  1:272386/93‑1 (MQ=255)

                                tGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGc                                                         >  1:308384/1‑93 (MQ=255)

                                  aCCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCat                                                       <  1:321565/93‑1 (MQ=255)

                                  aCCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCat                                                       <  2:4670104/93‑1 (MQ=255)

                                  aCCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCat                                                       >  1:3211762/1‑93 (MQ=255)

                                   ccGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCata                                                      <  1:3796628/93‑1 (MQ=255)

                                   ccGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCata                                                      >  2:4136369/1‑93 (MQ=255)

                                   ccGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCata                                                      >  2:219614/1‑93 (MQ=255)

                                    cGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCatat                                                     <  1:1669353/93‑1 (MQ=255)

                                    cGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCatat                                                     >  1:2238254/1‑93 (MQ=255)

                                    cGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCatat                                                     >  1:4404549/1‑93 (MQ=255)

                                    cGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCatat                                                     <  2:505224/93‑1 (MQ=255)

                                      tAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATAc                                                   <  2:2838538/93‑1 (MQ=255)

                                      tAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATAc                                                   >  1:2885094/1‑93 (MQ=255)

                                        aGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACga                                                 <  1:4648820/93‑1 (MQ=255)

                                        aGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACga                                                 >  1:1691699/1‑93 (MQ=255)

                                         gTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACgag                                                <  1:1076483/93‑1 (MQ=255)

                                         gTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACAGAGACAGGTCATCAGGAAGGAAACCATCAAGCCAATCAGCATATACgag                                                >  2:3328998/1‑93 (MQ=255)

                                          tGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACgaga                                               <  2:4681846/93‑1 (MQ=255)

                                            gCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGc                                             >  2:272162/1‑93 (MQ=255)

                                            gCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGc                                             <  1:2388086/93‑1 (MQ=255)

                                             cAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCa                                            >  1:934077/1‑93 (MQ=255)

                                              aaaCGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCaa                                           <  1:4453111/93‑1 (MQ=255)

                                               aaCGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCaag                                          >  1:1361067/1‑92 (MQ=255)

                                               aaCGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAt                                          >  1:1233485/1‑93 (MQ=255)

                                               aaCGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAt                                          >  1:1977697/1‑93 (MQ=255)

                                               aaCGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAt                                          >  1:1977698/1‑93 (MQ=255)

                                               aaCGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAt                                          <  2:1692355/93‑1 (MQ=255)

                                               aaCGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAt                                          <  1:2976663/93‑1 (MQ=255)

                                                   aaCCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGccc                                      >  2:4364153/1‑93 (MQ=255)

                                                   aaCCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGccc                                      <  2:801370/93‑1 (MQ=255)

                                                    aCCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGcccc                                     <  1:2345068/93‑1 (MQ=255)

                                                       ggAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCcccgcc                                  <  2:1313878/93‑1 (MQ=255)

                                                          aaCCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCgg                               >  2:2452456/1‑93 (MQ=255)

                                                           aCCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGa                              >  2:138651/1‑93 (MQ=255)

                                                            ccGGCATATATGCAGCCAGTTCACCGATACCGGTCATCAGGAAGTAAACCATCAAGCCAATCAGCATATACGAGAGCAATCGCCAGCCCGGAc                             >  2:3570069/1‑93 (MQ=255)

                                                            ccGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAc                             >  2:3432641/1‑93 (MQ=255)

                                                            ccGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAc                             >  1:2259186/1‑93 (MQ=255)

                                                             cGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcc                            <  2:2079918/93‑1 (MQ=255)

                                                               gCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctg                          >  1:368233/1‑93 (MQ=255)

                                                               gCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctg                          >  2:3401234/1‑93 (MQ=255)

                                                                cATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgc                         >  2:1133847/1‑93 (MQ=255)

                                                                cATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgc                         >  1:119138/1‑93 (MQ=255)

                                                                  tatatGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcct                       >  1:592726/1‑93 (MQ=255)

                                                                    tttGCAGCCAGTTCACCGAGACCGATCACCAGGAAGTAAACCATCAGGCCAGTCAGCATATACGAGAGACACGCCCCGCGCGGACCTGCTTga                     <  2:4193176/91‑1 (MQ=255)

                                                                    tatGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTga                     >  1:4649428/1‑93 (MQ=255)

                                                                    tatGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTga                     <  1:1396635/93‑1 (MQ=255)

                                                                         aGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAAt                >  1:2021653/1‑93 (MQ=255)

                                                                          gCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATc               <  2:3761016/93‑1 (MQ=255)

                                                                            cAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGt             >  2:2797140/1‑93 (MQ=255)

                                                                              gTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTg           >  2:4661780/1‑93 (MQ=255)

                                                                              gTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTg           <  2:2556113/93‑1 (MQ=255)

                                                                              gTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTg           <  2:4318422/93‑1 (MQ=255)

                                                                                 cACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTgcgc        >  1:3806156/1‑93 (MQ=255)

                                                                                 cACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTgcgc        >  1:1262375/1‑93 (MQ=255)

                                                                                  aCCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGcc       <  2:1926952/93‑1 (MQ=255)

                                                                                  aCCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGcc       >  2:3930268/1‑93 (MQ=255)

                                                                                  aCCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACATGCCTGAGAAATCGTTGCGcc       >  2:1521993/1‑93 (MQ=255)

                                                                                   ccGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCa      <  1:4050814/93‑1 (MQ=255)

                                                                                   ccGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCa      >  1:2561490/1‑93 (MQ=255)

                                                                                    cGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCag     >  1:4362758/1‑93 (MQ=255)

                                                                                     gagaCCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCaga    >  1:980223/1‑93 (MQ=255)

                                                                                     gagaCCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCaga    >  1:4146973/1‑93 (MQ=255)

                                                                                      agaCCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCagag   >  1:3682793/1‑93 (MQ=255)

                                                                                       gaCCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAgg  <  1:1907393/93‑1 (MQ=255)

                                                                                       gaCCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAgg  >  1:2091437/1‑93 (MQ=255)

GCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGG  >  NC_000913/2246261‑2246440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAAC  >  NC_000913/2246258‑2246444

CCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACC                                                                                               >  SRR4302544.3481727/1‑94 (MQ=60)

 CAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCG                                                                                              <  SRR4302544.2922882/94‑1 (MQ=60)

      CGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCAT                                                                                         <  SRR4302544.1003863/94‑1 (MQ=60)

      CGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCAT                                                                                         >  SRR4302544.2406953/1‑94 (MQ=60)

       GAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATC                                                                                        >  SRR4302544.2842427/1‑94 (MQ=60)

       GAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATC                                                                                        >  SRR4302544.1208609/1‑94 (MQ=60)

        AAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCA                                                                                       <  SRR4302544.4586233/94‑1 (MQ=60)

        AAGCCAAAGCCTTCTTCAACAGAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCA                                                                                       <  SRR4302544.2145417/94‑1 (MQ=60)

          GCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGG                                                                                     >  SRR4302544.1319074/1‑94 (MQ=60)

           CCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGA                                                                                    >  SRR4302544.4281607/1‑94 (MQ=60)

           CCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGA                                                                                    >  SRR4302544.3596672/1‑94 (MQ=60)

           CCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGA                                                                                    <  SRR4302544.3967322/94‑1 (MQ=60)

             AAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAG                                                                                  >  SRR4302544.3387017/1‑94 (MQ=60)

             AAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAG                                                                                  >  SRR4302544.446469/1‑94 (MQ=60)

              AAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGT                                                                                 >  SRR4302544.1790987/1‑94 (MQ=60)

               AGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTA                                                                                <  SRR4302544.1097799/94‑1 (MQ=60)

                GCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAA                                                                               >  SRR4302544.53870/1‑94 (MQ=60)

                GCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAA                                                                               >  SRR4302544.1789604/1‑94 (MQ=60)

                  CTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAAC                                                                             >  SRR4302544.643438/1‑94 (MQ=60)

                   TTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACC                                                                            <  SRR4302544.1900610/94‑1 (MQ=60)

                   TTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACC                                                                            <  SRR4302544.3746161/94‑1 (MQ=60)

                    TCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCA                                                                           >  SRR4302544.1199135/1‑94 (MQ=60)

                       TCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCA                                                                        <  SRR4302544.2400582/94‑1 (MQ=60)

                       TCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTAACCGAGACCGGTCATCAGGAAGTAAACCATCA                                                                        <  SRR4302544.137991/94‑1 (MQ=60)

                           CATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCC                                                                    <  SRR4302544.591907/94‑1 (MQ=60)

                           CATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCC                                                                    <  SRR4302544.3546047/94‑1 (MQ=60)

                           CATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCC                                                                    <  SRR4302544.1829199/94‑1 (MQ=60)

                            ATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCA                                                                   >  SRR4302544.3540896/1‑94 (MQ=60)

                             TAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAA                                                                  <  SRR4302544.2920551/94‑1 (MQ=60)

                               GTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATC                                                                <  SRR4302544.2768228/94‑1 (MQ=60)

                                TTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGTAAGTAAACCATCAGGCCAATCA                                                               <  SRR4302544.554446/94‑1 (MQ=60)

                                TTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCA                                                               >  SRR4302544.4599783/1‑94 (MQ=60)

                                 TCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAG                                                              <  SRR4302544.1529271/94‑1 (MQ=60)

                                 GCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAG                                                              <  SRR4302544.2289923/94‑1 (MQ=60)

                                  CTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGC                                                             >  SRR4302544.311585/1‑94 (MQ=60)

                                  CTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGC                                                             <  SRR4302544.275250/94‑1 (MQ=60)

                                    GACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCAT                                                           >  SRR4302544.3242391/1‑94 (MQ=60)

                                     ACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATA                                                          <  SRR4302544.324905/94‑1 (MQ=60)

                                      CCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATAT                                                         >  SRR4302544.2259315/1‑94 (MQ=60)

                                      CCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATAT                                                         >  SRR4302544.4449038/1‑94 (MQ=60)

                                      CCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATAT                                                         <  SRR4302544.3834222/94‑1 (MQ=60)

                                      CCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATAT                                                         <  SRR4302544.4157325/94‑1 (MQ=60)

                                       CGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATA                                                        <  SRR4302544.1685798/94‑1 (MQ=60)

                                        GTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATAC                                                       >  SRR4302544.2912916/1‑94 (MQ=60)

                                          AAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGA                                                     >  SRR4302544.1708393/1‑94 (MQ=60)

                                           AGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAG                                                    <  SRR4302544.4694837/94‑1 (MQ=60)

                                            GTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGA                                                   <  SRR4302544.1087185/94‑1 (MQ=60)

                                               GCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCA                                                <  SRR4302544.2410699/94‑1 (MQ=60)

                                               GCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCA                                                >  SRR4302544.943278/1‑94 (MQ=60)

                                                 AAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAT                                              >  SRR4302544.387985/1‑94 (MQ=60)

                                                 AAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAT                                              >  SRR4302544.1245683/1‑94 (MQ=60)

                                                 AAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAT                                              <  SRR4302544.4497965/94‑1 (MQ=60)

                                                 AAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAT                                              >  SRR4302544.1996916/1‑94 (MQ=60)

                                                 AAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAT                                              >  SRR4302544.1996917/1‑94 (MQ=60)

                                                 AAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAG                                              >  SRR4302544.1374582/1‑94 (MQ=60)

                                                  AACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATG                                             <  SRR4302544.3005215/94‑1 (MQ=60)

                                                   ACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCCGGCCAATCAGCATATACGAGAGCAATGC                                            <  SRR4302544.1245272/94‑1 (MQ=60)

                                                       ACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCG                                        <  SRR4302544.2367286/94‑1 (MQ=60)

                                                        CCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGC                                       <  SRR4302544.4349647/94‑1 (MQ=60)

                                                         CGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCC                                      <  SRR4302544.1308726/94‑1 (MQ=60)

                                                           GAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCG                                    <  SRR4302544.1683457/94‑1 (MQ=60)

                                                              ACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAC                                 >  SRR4302544.2280438/1‑94 (MQ=60)

                                                                 GGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTG                              >  SRR4302544.371996/1‑94 (MQ=60)

                                                                  GCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGC                             >  SRR4302544.1846891/1‑94 (MQ=60)

                                                                  GCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGC                             >  SRR4302544.120217/1‑94 (MQ=60)

                                                                    ATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCT                           >  SRR4302544.598546/1‑94 (MQ=60)

                                                                    ATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCT                           >  SRR4302544.2010149/1‑94 (MQ=60)

                                                                    ATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCT                           >  SRR4302544.569014/1‑94 (MQ=60)

                                                                      ATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGA                         >  SRR4302544.4695447/1‑94 (MQ=60)

                                                                       TATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAG                        <  SRR4302544.1410557/94‑1 (MQ=60)

                                                                         TGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAA                      <  SRR4302544.2503671/94‑1 (MQ=60)

                                                                           CAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAAT                    >  SRR4302544.2041270/1‑94 (MQ=60)

                                                                                AGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTG               >  SRR4302544.3161381/1‑94 (MQ=60)

                                                                                  TTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCG             <  SRR4302544.1991956/94‑1 (MQ=60)

                                                                                   TCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGC            >  SRR4302544.3843873/1‑94 (MQ=60)

                                                                                   TCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGC            >  SRR4302544.1274801/1‑94 (MQ=60)

                                                                                     ACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCA          >  SRR4302544.2586095/1‑94 (MQ=60)

                                                                                      CCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAG         <  SRR4302544.4091156/94‑1 (MQ=60)

                                                                                      CCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAG         >  SRR4302544.4407023/1‑94 (MQ=60)

                                                                                       CGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGA        >  SRR4302544.989826/1‑94 (MQ=60)

                                                                                       CGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGA        >  SRR4302544.4188031/1‑94 (MQ=60)

                                                                                        GAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAG       >  SRR4302544.3719515/1‑94 (MQ=60)

                                                                                         AGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGG      >  SRR4302544.2111563/1‑94 (MQ=60)

                                                                                          GACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGC     <  SRR4302544.1925836/94‑1 (MQ=60)

                                                                                           ACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCA    <  SRR4302544.1622799/94‑1 (MQ=60)

                                                                                             CGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAAC  >  SRR4302544.2441666/1‑94 (MQ=60)

CCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAAC  >  NC_000913/2246258‑2246444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`