Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,246,351	T→C	S68G (AGT→GGT)	lysP ←	lysine‑specific permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,246,351	0	T	C	99.6%	856.6 / ‑4.8	265	S68G (AGT→GGT)	lysP	lysine‑specific permease
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (139/125); total (139/126)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.97e-01

Only 100 of 267 total aligned reads displayed.

GCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGG  >  NC_000913/2246263‑2246440

  gAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGtcat                                                                                     <  1:1139612/93‑1 (MQ=255)

  gAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGtcat                                                                                     >  2:5691333/1‑93 (MQ=255)

   aaGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGtcatc                                                                                    >  1:928124/1‑93 (MQ=255)

    aGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGtcatca                                                                                   <  1:145539/93‑1 (MQ=255)

    aGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGtcatca                                                                                   >  2:1991776/1‑93 (MQ=255)

      ccAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAgg                                                                                 >  2:5029804/1‑93 (MQ=255)

       cAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGa                                                                                >  1:1215467/1‑93 (MQ=255)

       cAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGa                                                                                >  2:6637121/1‑93 (MQ=255)

       cAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGa                                                                                >  2:4581483/1‑93 (MQ=255)

        aaaGCCTTTTTCAACATAGTTCTGACCGTAAGTGGCAAACGGACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGaa                                                                               >  1:6512295/1‑93 (MQ=255)

        aaaGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGaa                                                                               <  2:3114783/93‑1 (MQ=255)

        aaaGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGaa                                                                               >  1:2366017/1‑93 (MQ=255)

         aaGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAg                                                                              >  2:1031041/1‑93 (MQ=255)

         aaGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAg                                                                              >  2:380609/1‑93 (MQ=255)

          aGCCTTCTTCAACATAGTTCTGACCGTAAGTGACAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGt                                                                             >  1:772523/1‑93 (MQ=255)

            ccTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTaa                                                                           >  1:1020248/1‑93 (MQ=255)

             cttcttCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTaaa                                                                          >  2:1812223/1‑93 (MQ=255)

             cttcttCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTaaa                                                                          >  2:2332157/1‑93 (MQ=255)

             cttcttCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTaaa                                                                          >  1:3368705/1‑93 (MQ=255)

              ttcttcAACATAGTTCTGTCCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAAc                                                                         <  2:5526945/93‑1 (MQ=255)

              ttcttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAAc                                                                         <  1:986279/93‑1 (MQ=255)

              ttcttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAAc                                                                         >  2:860795/1‑93 (MQ=255)

               tcttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAAcc                                                                        <  2:6137823/93‑1 (MQ=255)

                cttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAAcaa                                                                       >  2:3058449/1‑91 (MQ=255)

                 ttcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCAt                                                                      >  1:3914690/1‑93 (MQ=255)

                  tcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATc                                                                     <  2:3316188/93‑1 (MQ=255)

                  tcAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATc                                                                     <  1:1798743/93‑1 (MQ=255)

                   cAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCa                                                                    <  2:2422990/93‑1 (MQ=255)

                   cAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCa                                                                    <  2:5283955/93‑1 (MQ=255)

                    aaCATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAg                                                                   >  1:5019587/1‑93 (MQ=255)

                    aaCATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAg                                                                   >  1:3711090/1‑93 (MQ=255)

                     aCATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAgg                                                                  <  2:6390139/93‑1 (MQ=255)

                        tAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCa                                                               >  1:1700503/1‑93 (MQ=255)

                        tAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCa                                                               >  2:4680144/1‑93 (MQ=255)

                          gTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAAt                                                             <  1:5032994/93‑1 (MQ=255)

                           ttCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATc                                                            >  1:1339582/1‑93 (MQ=255)

                           ttCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATc                                                            >  2:1385543/1‑93 (MQ=255)

                             cTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAg                                                          <  1:2692334/93‑1 (MQ=255)

                               gACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCa                                                        <  1:6408444/93‑1 (MQ=255)

                               gACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCa                                                        >  2:1598893/1‑93 (MQ=255)

                                aCCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCat                                                       <  2:6061890/93‑1 (MQ=255)

                                aCCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCat                                                       <  1:1288072/93‑1 (MQ=255)

                                  cGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCatat                                                     >  2:1701533/1‑93 (MQ=255)

                                   gTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCAtata                                                    >  1:3003843/1‑93 (MQ=255)

                                    tAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATAc                                                   <  1:2957995/93‑1 (MQ=255)

                                    tAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATAc                                                   <  1:1305138/93‑1 (MQ=255)

                                      aGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACga                                                 <  1:5744430/93‑1 (MQ=255)

                                      aGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACga                                                 <  1:6578334/93‑1 (MQ=255)

                                        tGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACgaga                                               <  2:3030675/93‑1 (MQ=255)

                                         ggCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGagag                                              <  2:128175/93‑1 (MQ=255)

                                         ggCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGagag                                              <  2:75581/93‑1 (MQ=255)

                                         ggCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGagag                                              >  2:2690979/1‑93 (MQ=255)

                                          gCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGc                                             <  2:5438108/93‑1 (MQ=255)

                                          gCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGc                                             <  1:3624431/93‑1 (MQ=255)

                                          gCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGc                                             <  1:4665887/93‑1 (MQ=255)

                                              aCGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATg                                         >  1:2910775/1‑93 (MQ=255)

                                                gAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGcc                                       <  2:3870250/93‑1 (MQ=255)

                                                gAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGcc                                       <  1:4059686/93‑1 (MQ=255)

                                                 aaCCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGccc                                      <  1:2201363/93‑1 (MQ=255)

                                                 aaCCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGccc                                      >  1:4749809/1‑93 (MQ=255)

                                                    cGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCcccgc                                   >  1:2528598/1‑93 (MQ=255)

                                                     ggAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCcccgcc                                  >  2:3517346/1‑93 (MQ=255)

                                                     ggAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCcccgcc                                  >  1:1942/1‑93 (MQ=255)

                                                        aaCCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCgg                               >  2:4166919/1‑93 (MQ=255)

                                                          ccGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAc                             >  2:2152150/1‑93 (MQ=255)

                                                          ccGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAc                             <  2:4133575/93‑1 (MQ=255)

                                                            ggCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcct                           >  2:3212833/1‑93 (MQ=255)

                                                             gCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctg                          >  2:6455/1‑93 (MQ=255)

                                                               atatatGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcc                        >  1:2868775/1‑93 (MQ=255)

                                                               atatatGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATAAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcc                        <  2:4322105/93‑1 (MQ=255)

                                                                tatatGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcct                       <  2:6152738/93‑1 (MQ=255)

                                                                tatatGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcct                       <  2:3688112/93‑1 (MQ=255)

                                                                tatatGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcct                       >  1:6082499/1‑93 (MQ=255)

                                                                tatatGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAcctgcct                       >  1:5400008/1‑93 (MQ=255)

                                                                   atGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTgag                    <  2:5749514/93‑1 (MQ=255)

                                                                    tGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTgaga                   <  2:2086351/93‑1 (MQ=255)

                                                                    tGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTgaga                   >  2:4467762/1‑93 (MQ=255)

                                                                      cAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCACCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGaaa                 <  2:1768889/93‑1 (MQ=255)

                                                                        gCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGGCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATc               >  1:5236528/1‑93 (MQ=255)

                                                                        gCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATc               >  1:558991/1‑93 (MQ=255)

                                                                        gCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATc               >  1:4704193/1‑93 (MQ=255)

                                                                        gCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATc               >  1:4089536/1‑93 (MQ=255)

                                                                        gCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATc               <  2:2242506/93‑1 (MQ=255)

                                                                          cAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGt             <  1:5543482/93‑1 (MQ=255)

                                                                          cAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGt             <  2:5777145/93‑1 (MQ=255)

                                                                          cAGTCCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGt             <  1:5291363/93‑1 (MQ=255)

                                                                           aGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGtt            <  1:3484482/93‑1 (MQ=255)

                                                                           aGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGtt            <  1:3220013/93‑1 (MQ=255)

                                                                            gTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTg           >  1:207635/1‑93 (MQ=255)

                                                                              tCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTgcc         >  1:6099417/1‑92 (MQ=255)

                                                                               cACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTgcgc        >  1:3021030/1‑93 (MQ=255)

                                                                               cACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTgcgc        <  2:3427119/93‑1 (MQ=255)

                                                                                aCCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGcc       >  1:4382187/1‑93 (MQ=255)

                                                                                aCCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGcc       >  2:652330/1‑93 (MQ=255)

                                                                                 ccGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCa      >  2:5903256/1‑93 (MQ=255)

                                                                                   gagaCCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCaga    >  2:6626450/1‑93 (MQ=255)

                                                                                    agaCCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCagag   >  1:5102760/1‑93 (MQ=255)

                                                                                    agaCCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCagag   >  1:3303047/1‑93 (MQ=255)

                                                                                     gaCCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAgg  >  2:4941185/1‑93 (MQ=255)

                                                                                     gaCCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAgg  >  2:2816069/1‑93 (MQ=255)

GCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGG  >  NC_000913/2246263‑2246440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAAC  >  NC_000913/2246258‑2246444

CCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACC                                                                                               <  SRR4302536.6850533/94‑1 (MQ=60)

CCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACC                                                                                               >  SRR4302536.1350831/1‑94 (MQ=60)

 CAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCG                                                                                              <  SRR4302536.667191/94‑1 (MQ=60)

      CGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCAT                                                                                         >  SRR4302536.2472418/1‑94 (MQ=60)

       GAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATC                                                                                        <  SRR4302536.1010537/94‑1 (MQ=60)

       GAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATC                                                                                        >  SRR4302536.955474/1‑94 (MQ=60)

       GAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATC                                                                                        <  SRR4302536.1173874/94‑1 (MQ=60)

       GAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATC                                                                                        >  SRR4302536.4161479/1‑94 (MQ=60)

        AAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCA                                                                                       <  SRR4302536.4205330/94‑1 (MQ=60)

         AGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAG                                                                                      >  SRR4302536.4870697/1‑94 (MQ=60)

         AGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAG                                                                                      <  SRR4302536.149337/94‑1 (MQ=60)

          GCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGG                                                                                     >  SRR4302536.5229461/1‑94 (MQ=60)

          GCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGG                                                                                     >  SRR4302536.327324/1‑94 (MQ=60)

           CCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGA                                                                                    >  SRR4302536.1251982/1‑94 (MQ=60)

            CAAAGCCTTTTTCAACATAGTTCTGACCGTAAGTGGCAAACGGACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAA                                                                                   >  SRR4302536.6706348/1‑94 (MQ=60)

            CAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAA                                                                                   >  SRR4302536.2437722/1‑94 (MQ=60)

            CAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAA                                                                                   >  SRR4302536.2199231/1‑94 (MQ=60)

            CAAAGCCTTCTTCAACATAGTCCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAA                                                                                   <  SRR4302536.2981685/94‑1 (MQ=60)

             AAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAG                                                                                  <  SRR4302536.1768822/94‑1 (MQ=60)

              AAGCCTTCTTCAACATAGTTCTGACCGTAAGTGACAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGT                                                                                 >  SRR4302536.795175/1‑94 (MQ=60)

                GCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAA                                                                               >  SRR4302536.515141/1‑94 (MQ=60)

                GCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAA                                                                               >  SRR4302536.1050451/1‑94 (MQ=60)

                 CCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAA                                                                              >  SRR4302536.3468781/1‑94 (MQ=60)

                 CCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAA                                                                              >  SRR4302536.2287321/1‑94 (MQ=60)

                   TTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACC                                                                            <  SRR4302536.1015382/94‑1 (MQ=60)

                     CTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCAT                                                                          >  SRR4302536.4031062/1‑94 (MQ=60)

                     CTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCAT                                                                          >  SRR4302536.3742423/1‑94 (MQ=60)

                       TCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCA                                                                        <  SRR4302536.1853038/94‑1 (MQ=60)

                       TCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCA                                                                        <  SRR4302536.2799748/94‑1 (MQ=60)

                        CAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAG                                                                       >  SRR4302536.5170059/1‑94 (MQ=60)

                        CAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAG                                                                       >  SRR4302536.3973892/1‑94 (MQ=60)

                        CAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAG                                                                       >  SRR4302536.3822032/1‑94 (MQ=60)

                         AACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGG                                                                      >  SRR4302536.3528290/1‑94 (MQ=60)

                         AACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGG                                                                      <  SRR4302536.2825711/94‑1 (MQ=60)

                            ATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCA                                                                   >  SRR4302536.1749713/1‑94 (MQ=60)

                            ATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCA                                                                   <  SRR4302536.6773696/94‑1 (MQ=60)

                             TAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAA                                                                  <  SRR4302536.147041/94‑1 (MQ=60)

                               GTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATC                                                                <  SRR4302536.5183859/94‑1 (MQ=60)

                               GTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATC                                                                >  SRR4302536.1379534/1‑94 (MQ=60)

                                 TCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAG                                                              <  SRR4302536.5503870/94‑1 (MQ=60)

                                 TCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAG                                                              <  SRR4302536.5341671/94‑1 (MQ=60)

                                  CTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGC                                                             <  SRR4302536.2772705/94‑1 (MQ=60)

                                    GACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCAT                                                           <  SRR4302536.3053759/94‑1 (MQ=60)

                                    GACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCAT                                                           <  SRR4302536.6611375/94‑1 (MQ=60)

                                    GACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCAT                                                           <  SRR4302536.6599851/94‑1 (MQ=60)

                                     ACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATA                                                          >  SRR4302536.796474/1‑94 (MQ=60)

                                     ACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATA                                                          <  SRR4302536.1326604/94‑1 (MQ=60)

                                      CCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATAT                                                         <  SRR4302536.5857695/94‑1 (MQ=60)

                                      CCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATAT                                                         <  SRR4302536.1200503/94‑1 (MQ=60)

                                      CCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATAT                                                         >  SRR4302536.6271871/1‑94 (MQ=60)

                                       CGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATA                                                        >  SRR4302536.3092175/1‑94 (MQ=60)

                                        GTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATAC                                                       <  SRR4302536.5822025/94‑1 (MQ=60)

                                        GTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATAC                                                       >  SRR4302536.430910/1‑94 (MQ=60)

                                        GTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATAC                                                       >  SRR4302536.3231652/1‑94 (MQ=60)

                                        CTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATAC                                                       <  SRR4302536.4165186/94‑1 (MQ=60)

                                         TAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACG                                                      <  SRR4302536.3045146/94‑1 (MQ=60)

                                         TAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACG                                                      <  SRR4302536.1344113/94‑1 (MQ=60)

                                         TAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACG                                                      <  SRR4302536.5679402/94‑1 (MQ=60)

                                          AAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGA                                                     >  SRR4302536.1800978/1‑94 (MQ=60)

                                          AAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGA                                                     >  SRR4302536.142564/1‑94 (MQ=60)

                                           AGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAG                                                    <  SRR4302536.5915306/94‑1 (MQ=60)

                                           AGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAG                                                    <  SRR4302536.6774101/94‑1 (MQ=60)

                                           AGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAG                                                    <  SRR4302536.2399084/94‑1 (MQ=60)

                                           AGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAG                                                    >  SRR4302536.6824809/1‑94 (MQ=60)

                                            GTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGA                                                   >  SRR4302536.3097251/1‑94 (MQ=60)

                                             TGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAG                                                  <  SRR4302536.4830858/94‑1 (MQ=60)

                                             TGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAG                                                  >  SRR4302536.1206611/1‑94 (MQ=60)

                                               GCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCA                                                <  SRR4302536.3732821/94‑1 (MQ=60)

                                               GCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCA                                                <  SRR4302536.4806171/94‑1 (MQ=60)

                                                CAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAA                                               <  SRR4302536.5130263/94‑1 (MQ=60)

                                                CAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAA                                               >  SRR4302536.4706739/1‑94 (MQ=60)

                                                 AAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAT                                              <  SRR4302536.3475255/94‑1 (MQ=60)

                                                 AAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAT                                              >  SRR4302536.136880/1‑94 (MQ=60)

                                                 AAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAT                                              >  SRR4302536.551657/1‑94 (MQ=60)

                                                 AAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAT                                              <  SRR4302536.3034912/94‑1 (MQ=60)

                                                 AAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAAT                                              >  SRR4302536.1945055/1‑94 (MQ=60)

                                                  AACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATG                                             <  SRR4302536.3439022/94‑1 (MQ=60)

                                                  AACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATG                                             <  SRR4302536.901211/94‑1 (MQ=60)

                                                  AACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATG                                             >  SRR4302536.651468/1‑94 (MQ=60)

                                                  AACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATG                                             <  SRR4302536.3324748/94‑1 (MQ=60)

                                                  AACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATG                                             >  SRR4302536.2996697/1‑94 (MQ=60)

                                                    CGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCC                                           <  SRR4302536.6528559/94‑1 (MQ=60)

                                                     GAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCC                                          <  SRR4302536.4180019/94‑1 (MQ=60)

                                                     GAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCC                                          >  SRR4302536.4892757/1‑94 (MQ=60)

                                                     GAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCC                                          <  SRR4302536.5582926/94‑1 (MQ=60)

                                                      AACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATAGGAGAGAAATGCCCC                                         >  SRR4302536.780394/1‑94 (MQ=60)

                                                      AACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCC                                         <  SRR4302536.3864741/94‑1 (MQ=60)

                                                      AACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCC                                         <  SRR4302536.2267823/94‑1 (MQ=60)

                                                      AACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCC                                         >  SRR4302536.2229847/1‑94 (MQ=60)

                                                       ACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCG                                        <  SRR4302536.4716863/94‑1 (MQ=60)

                                                        CCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGC                                       >  SRR4302536.2604822/1‑94 (MQ=60)

                                                        CCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGC                                       <  SRR4302536.2039493/94‑1 (MQ=60)

                                                         CGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCC                                      >  SRR4302536.1996/1‑94 (MQ=60)

                                                         CGGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCC                                      >  SRR4302536.1966101/1‑94 (MQ=60)

                                                          GGAAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCC                                     >  SRR4302536.1299514/1‑94 (MQ=60)

                                                            AAACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCAAATACGAGGGCAATGCCCCGCCCGG                                   >  SRR4302536.1354333/1‑94 (MQ=60)

                                                              ACCGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGAC                                 <  SRR4302536.5387109/94‑1 (MQ=60)

                                                                CGGCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCT                               <  SRR4302536.5414343/94‑1 (MQ=60)

                                                                  GCATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGC                             <  SRR4302536.6661035/94‑1 (MQ=60)

                                                                   CATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCC                            >  SRR4302536.1019897/1‑94 (MQ=60)

                                                                   CATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCC                            >  SRR4302536.2749145/1‑94 (MQ=60)

                                                                   CATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCC                            >  SRR4302536.2953503/1‑94 (MQ=60)

                                                                    ATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCT                           >  SRR4302536.5561310/1‑94 (MQ=60)

                                                                    ATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCT                           >  SRR4302536.6263929/1‑94 (MQ=60)

                                                                    ATATATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCT                           <  SRR4302536.4387018/94‑1 (MQ=60)

                                                                       TATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAG                        <  SRR4302536.5127253/94‑1 (MQ=60)

                                                                        ATGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGA                       >  SRR4302536.1541909/1‑94 (MQ=60)

                                                                         TGCAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAA                      >  SRR4302536.2721269/1‑94 (MQ=60)

                                                                           CAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAAT                    >  SRR4302536.1332740/1‑94 (MQ=60)

                                                                           CAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAAT                    >  SRR4302536.3020371/1‑94 (MQ=60)

                                                                           CAGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAAT                    >  SRR4302536.331823/1‑94 (MQ=60)

                                                                            AGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGGCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATC                   >  SRR4302536.5392546/1‑94 (MQ=60)

                                                                            AGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATC                   >  SRR4302536.5212364/1‑94 (MQ=60)

                                                                            AGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATC                   >  SRR4302536.4210779/1‑94 (MQ=60)

                                                                            AGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATC                   >  SRR4302536.573232/1‑94 (MQ=60)

                                                                            AGCCAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATC                   >  SRR4302536.4845653/1‑94 (MQ=60)

                                                                               CAGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTT                <  SRR4302536.5708673/94‑1 (MQ=60)

                                                                               CAGTCCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTT                <  SRR4302536.5449142/94‑1 (MQ=60)

                                                                                AGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTG               >  SRR4302536.213112/1‑94 (MQ=60)

                                                                                AGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTG               <  SRR4302536.1062335/94‑1 (MQ=60)

                                                                                AGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTG               >  SRR4302536.1252876/1‑94 (MQ=60)

                                                                                AGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTG               <  SRR4302536.3314437/94‑1 (MQ=60)

                                                                                AGTTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTG               <  SRR4302536.3588396/94‑1 (MQ=60)

                                                                                AGTGCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTG               <  SRR4302536.5945002/94‑1 (MQ=60)

                                                                                  TTCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCC             >  SRR4302536.6281276/1‑94 (MQ=60)

                                                                                   TCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGC            >  SRR4302536.3067631/1‑94 (MQ=60)

                                                                                   TCACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGC            >  SRR4302536.3109796/1‑94 (MQ=60)

                                                                                    CACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCC           <  SRR4302536.6920623/94‑1 (MQ=60)

                                                                                    CACCGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCC           >  SRR4302536.4513025/1‑94 (MQ=60)

                                                                                       CGAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGA        >  SRR4302536.5737179/1‑94 (MQ=60)

                                                                                        GAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAG       >  SRR4302536.3609351/1‑94 (MQ=60)

                                                                                        GAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAG       <  SRR4302536.4887099/94‑1 (MQ=60)

                                                                                        GAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAG       >  SRR4302536.3400501/1‑94 (MQ=60)

                                                                                        GAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAG       >  SRR4302536.5255320/1‑94 (MQ=60)

                                                                                        GAGACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAG       >  SRR4302536.3609301/1‑94 (MQ=60)

                                                                                          GACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGC     <  SRR4302536.6012448/94‑1 (MQ=60)

                                                                                           ACCGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCA    >  SRR4302536.3811352/1‑94 (MQ=60)

                                                                                             CGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAAC  <  SRR4302536.560131/94‑1 (MQ=60)

                                                                                             CGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAAC  <  SRR4302536.5011479/94‑1 (MQ=60)

                                                                                             CGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAAC  >  SRR4302536.2654879/1‑94 (MQ=60)

CCAGCGCGAAGCCAAAGCCTTCTTCAACATAGTTCTGACCGTAAGTGGCAAACGAACCGGAAACCGGCATATATGCAGCCAGTTCACCGAGACTGGTCATCAGGAAGTAAACCATCAGGCCAATCAGCATATACGAGAGCAATGCCCCGCCCGGACCTGCCTGAGAAATCGTTGCGCCAGAGGCAAC  >  NC_000913/2246258‑2246444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`