Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,250,142	T→C	R141R (CGT→CGC)	yeiI →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,250,142	0	T	C	98.7%	494.0 / ‑4.2	154	R141R (CGT→CGC)	yeiI	putative kinase
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (62/90); total (63/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

TTTGCTTTCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGA  >  NC_000913/2250052‑2250231

tttGCTTTCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGctt                                                                                         >  2:1649856/1‑93 (MQ=255)

tttGCTTTCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGctt                                                                                         >  1:18741/1‑93 (MQ=255)

  tGCTTTCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCACTTGc                                                                                       <  2:1648521/93‑1 (MQ=255)

    cTTTCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCAt                                                                                     <  2:3831610/93‑1 (MQ=255)

    cTTTCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCAt                                                                                     <  1:183702/93‑1 (MQ=255)

     tttCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATg                                                                                    >  2:1600163/1‑93 (MQ=255)

     tttCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATg                                                                                    >  1:3944735/1‑93 (MQ=255)

        cAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTc                                                                                 <  1:2728436/93‑1 (MQ=255)

        cAGTGATTGGCGATGACTTTT‑ATGGCGAAAGGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTc                                                                                 <  1:2728435/93‑1 (MQ=255)

         aGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATTGTCa                                                                                >  1:1100081/1‑93 (MQ=255)

          gTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaa                                                                               >  2:446527/1‑93 (MQ=255)

          gTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaa                                                                               >  1:3459665/1‑93 (MQ=255)

          gTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaa                                                                               >  2:3054990/1‑93 (MQ=255)

          gTGATTGGC‑ATGGTTTTTGATGGCAAAATGCTCGTGGAAGAAGCGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaa                                                                               <  2:1872859/93‑1 (MQ=255)

           tGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaaa                                                                              <  2:2489989/93‑1 (MQ=255)

           ggATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaaa                                                                              <  1:3908646/92‑1 (MQ=255)

            gATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAg                                                                             >  1:1561469/1‑93 (MQ=255)

               tGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGcac                                                                          <  2:4093306/93‑1 (MQ=255)

                 gCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACAt                                                                        <  2:3991842/93‑1 (MQ=255)

                  cGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATc                                                                       <  2:2633198/93‑1 (MQ=255)

                  cGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATc                                                                       <  2:1262720/93‑1 (MQ=255)

                  cGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATc                                                                       <  1:3788710/93‑1 (MQ=255)

                   gATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCg                                                                      >  1:4258060/1‑93 (MQ=255)

                   gATGACTTTT‑ATGGCGAAATGCTACTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCg                                                                      <  2:2587584/93‑1 (MQ=255)

                    aTGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGa                                                                     >  1:261723/1‑93 (MQ=255)

                     tGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGAc                                                                    <  1:653433/93‑1 (MQ=255)

                     tGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGAc                                                                    <  2:61011/93‑1 (MQ=255)

                     tGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGAc                                                                    <  1:3777370/93‑1 (MQ=255)

                      gACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACg                                                                   <  2:484767/93‑1 (MQ=255)

                      gACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACg                                                                   <  2:1393226/93‑1 (MQ=255)

                       aCTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGt                                                                  >  2:1712630/1‑93 (MQ=255)

                         tttt‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTAt                                                                >  2:149529/1‑93 (MQ=255)

                         tttt‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTAt                                                                >  2:796661/1‑93 (MQ=255)

                         tttt‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGCTTCCGGCTGCGTTCGTTTGCATGGTCACAGCACATCGACGTAt                                                                >  1:738400/1‑93 (MQ=255)

                          ttt‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATc                                                               <  2:440373/93‑1 (MQ=255)

                           tt‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCt                                                              <  1:2902166/93‑1 (MQ=255)

                            t‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTg                                                             >  1:2054382/1‑93 (MQ=255)

                                   gAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAAtt                                                       >  2:3260651/1‑93 (MQ=255)

                                   gAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAAtt                                                       >  1:3606087/1‑93 (MQ=255)

                                     aaTGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGc                                                     >  2:2129291/1‑93 (MQ=255)

                                        gCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCaa                                                  >  2:4459431/1‑93 (MQ=255)

                                        gCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCaa                                                  <  1:510821/93‑1 (MQ=255)

                                          tCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATc                                                <  1:1575197/93‑1 (MQ=255)

                                          tCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATc                                                >  1:737854/1‑93 (MQ=255)

                                          tCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATc                                                <  1:2998441/93‑1 (MQ=255)

                                          tCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATc                                                >  2:4642351/1‑93 (MQ=255)

                                            cTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCga                                              >  1:2572567/1‑93 (MQ=255)

                                            cTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCga                                              >  2:3445580/1‑93 (MQ=255)

                                            cTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCga                                              <  2:3257478/93‑1 (MQ=255)

                                            cTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCga                                              <  1:2159047/93‑1 (MQ=255)

                                            cTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCga                                              >  1:4401014/1‑93 (MQ=255)

                                            cTGGAAGAAACGCGCCGTGACGGCGTGAATGGCTCCGGCTGCGTTCGCTTGCATGGTCAGAGCACATCGACGTATCTGGCAATTGCCAATCga                                              <  1:1646268/93‑1 (MQ=255)

                                             tGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCgag                                             <  2:3565749/93‑1 (MQ=255)

                                             tGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCgag                                             <  2:2506909/93‑1 (MQ=255)

                                               gaagaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGAc                                           <  2:429227/93‑1 (MQ=255)

                                               gaagaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGAc                                           >  2:2673174/1‑93 (MQ=255)

                                               gaagaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGAc                                           <  1:4112858/93‑1 (MQ=255)

                                                 agaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACgc                                         >  1:3083261/1‑92 (MQ=255)

                                                 agaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGa                                         <  1:2344791/93‑1 (MQ=255)

                                                  gaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGAt                                        <  1:500045/93‑1 (MQ=255)

                                                  gaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGAt                                        <  2:454968/93‑1 (MQ=255)

                                                  gaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCGGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGAt                                        <  1:4572417/93‑1 (MQ=255)

                                                   aaaCGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATc                                       <  1:3818821/93‑1 (MQ=255)

                                                      cgcgCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAACCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCaaa                                    <  2:3111621/93‑1 (MQ=255)

                                                       gcgcCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAAc                                   <  2:3065592/93‑1 (MQ=255)

                                                        cgcCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAAcc                                  <  2:612681/93‑1 (MQ=255)

                                                        cgcCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAAcc                                  <  1:2383620/93‑1 (MQ=255)

                                                        cgcCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAAcc                                  <  1:1705438/93‑1 (MQ=255)

                                                         gcCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCg                                 >  2:2709756/1‑93 (MQ=255)

                                                           cGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTg                               >  1:1053818/1‑93 (MQ=255)

                                                            gTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGc                              <  2:2269597/93‑1 (MQ=255)

                                                             tGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCt                             >  2:3529375/1‑93 (MQ=255)

                                                              gCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTg                            <  2:3502831/93‑1 (MQ=255)

                                                               ccGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTgg                           <  1:2375050/93‑1 (MQ=255)

                                                                cGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGc                          <  1:2677221/93‑1 (MQ=255)

                                                                cGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGc                          >  2:4269799/1‑93 (MQ=255)

                                                                    gTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGAtt                      >  1:2102467/1‑93 (MQ=255)

                                                                    gTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGAtt                      >  2:215263/1‑93 (MQ=255)

                                                                     tGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTa                     >  1:4491333/1‑93 (MQ=255)

                                                                        aTGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACg                  >  2:3832610/1‑93 (MQ=255)

                                                                         tGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGa                 <  1:3520205/93‑1 (MQ=255)

                                                                         tGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGa                 <  1:1722893/93‑1 (MQ=255)

                                                                            ctcCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAc              <  1:788680/93‑1 (MQ=255)

                                                                            ctcCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAc              <  2:3448040/93‑1 (MQ=255)

                                                                             tcCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAcc             >  2:1253842/1‑93 (MQ=255)

                                                                             tcCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAcc             >  1:3454591/1‑93 (MQ=255)

                                                                              ccGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAccc            <  2:1995540/93‑1 (MQ=255)

                                                                              ccGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAccc            <  1:2953895/93‑1 (MQ=255)

                                                                               cGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCa           <  2:529725/93‑1 (MQ=255)

                                                                                 gCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATc         <  2:1563331/93‑1 (MQ=255)

                                                                                 gCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATc         <  1:2760235/93‑1 (MQ=255)

                                                                                 gCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATc         <  1:3976318/93‑1 (MQ=255)

                                                                                  cTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATct        <  2:950298/93‑1 (MQ=255)

                                                                                  cTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATct        <  2:929465/93‑1 (MQ=255)

                                                                                     cgttcgctTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATctgct     >  1:1304821/1‑93 (MQ=255)

                                                                                      gttcgctTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATctgctg    >  1:4238156/1‑93 (MQ=255)

                                                                                      gttcgctTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATctgctg    >  2:2094081/1‑93 (MQ=255)

                                                                                       ttcgctTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTgg   >  1:253580/1‑93 (MQ=255)

                                                                                       ttcgctTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTgg   >  1:539671/1‑93 (MQ=255)

                                                                                        tcgctTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGa  >  1:4088467/1‑93 (MQ=255)

TTTGCTTTCAGTGATTGGCGATGACTTTT‑ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGA  >  NC_000913/2250052‑2250231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAG  >  NC_000913/2250050‑2250235

CATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCT                                                                                              >  SRR4302544.2909869/1‑94 (MQ=60)

 ATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTT                                                                                             >  SRR4302544.4097737/1‑94 (MQ=60)

 ATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTT                                                                                             <  SRR4302544.3253347/94‑1 (MQ=60)

 ATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTT                                                                                             >  SRR4302544.18927/1‑94 (MQ=60)

  TTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTG                                                                                            >  SRR4302544.3827326/1‑94 (MQ=60)

     GCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCAT                                                                                         <  SRR4302544.220565/94‑1 (MQ=60)

      CTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATG                                                                                        >  SRR4302544.3983929/1‑94 (MQ=60)

      CTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATG                                                                                        <  SRR4302544.185524/94‑1 (MQ=60)

          CAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATTGTCA                                                                                    >  SRR4302544.1111035/1‑94 (MQ=60)

          CAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCA                                                                                    <  SRR4302544.2754725/94‑1 (MQ=60)

          CAGTGATTGGCGATGACTTTTATGGCGAAAGGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCA                                                                                    <  SRR4302544.2754724/94‑1 (MQ=60)

          CAGTGATGGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCA                                                                                    <  SRR4302544.1886162/94‑1 (MQ=60)

           AGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAA                                                                                   >  SRR4302544.2659975/1‑94 (MQ=60)

           AGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAA                                                                                   >  SRR4302544.3492687/1‑94 (MQ=60)

             TGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAG                                                                                 >  SRR4302544.1576973/1‑94 (MQ=60)

             TGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGGCCAAG                                                                                 >  SRR4302544.3544013/1‑94 (MQ=60)

             GGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAG                                                                                 <  SRR4302544.3947481/94‑1 (MQ=60)

               ATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCA                                                                               >  SRR4302544.3559880/1‑94 (MQ=60)

               ATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCA                                                                               >  SRR4302544.2167583/1‑94 (MQ=60)

                 TGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACA                                                                             >  SRR4302544.281384/1‑94 (MQ=60)

                 TGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACA                                                                             >  SRR4302544.3902493/1‑94 (MQ=60)

                   GCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATC                                                                           <  SRR4302544.2782431/94‑1 (MQ=60)

                   GCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATC                                                                           <  SRR4302544.3301423/94‑1 (MQ=60)

                    CGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCG                                                                          <  SRR4302544.3826226/94‑1 (MQ=60)

                    CGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCG                                                                          >  SRR4302544.4301475/1‑94 (MQ=60)

                     GATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGA                                                                         >  SRR4302544.264491/1‑94 (MQ=60)

                      ATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGAC                                                                        >  SRR4302544.4299641/1‑94 (MQ=60)

                       TGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACG                                                                       <  SRR4302544.3814796/94‑1 (MQ=60)

                       TGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACG                                                                       <  SRR4302544.659902/94‑1 (MQ=60)

                          CTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTTCATGGTCCAAGCACATCGACGTAT                                                                    >  SRR4302544.791786/1‑94 (MQ=60)

                          CTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTAT                                                                    >  SRR4302544.2401967/1‑94 (MQ=60)

                          CTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTAT                                                                    >  SRR4302544.4672329/1‑94 (MQ=60)

                          CTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGCTTCCGGCTGCGTTCGTTTGCATGGTCACAGCACATCGACGTAT                                                                    >  SRR4302544.745624/1‑94 (MQ=60)

                           TTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATC                                                                   >  SRR4302544.257331/1‑94 (MQ=60)

                             TTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTG                                                                 <  SRR4302544.2930111/94‑1 (MQ=60)

                             TTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTG                                                                 <  SRR4302544.2930110/94‑1 (MQ=60)

                             TTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTG                                                                 >  SRR4302544.2074153/1‑94 (MQ=60)

                              TATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGG                                                                <  SRR4302544.3750655/94‑1 (MQ=60)

                               ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGC                                                               >  SRR4302544.3991186/1‑94 (MQ=60)

                                   CGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATT                                                           >  SRR4302544.3641113/1‑94 (MQ=60)

                                         GCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAAT                                                     <  SRR4302544.595886/94‑1 (MQ=60)

                                         GCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAAT                                                     <  SRR4302544.515861/94‑1 (MQ=60)

                                          CTCCTGGAAGAAACGCGCCGTGCCGGCGTGTATGGCTCCGGCTGGGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATG                                                    >  SRR4302544.3429945/1‑94 (MQ=60)

                                          CTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATC                                                    >  SRR4302544.745074/1‑94 (MQ=60)

                                           TCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCG                                                   <  SRR4302544.1590772/94‑1 (MQ=60)

                                           TCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCG                                                   <  SRR4302544.3027192/94‑1 (MQ=60)

                                            CCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGA                                                  >  SRR4302544.4445487/1‑94 (MQ=60)

                                            CCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGA                                                  >  SRR4302544.720691/1‑94 (MQ=60)

                                            CCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGA                                                  >  SRR4302544.12245/1‑94 (MQ=60)

                                            CCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGA                                                  >  SRR4302544.2597283/1‑94 (MQ=60)

                                             CTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAG                                                 <  SRR4302544.2179560/94‑1 (MQ=60)

                                             CTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAG                                                 <  SRR4302544.1662502/94‑1 (MQ=60)

                                             CTGGAAGAAACGCGCCGTGACGGCGTGAATGGCTCCGGCTGCGTTCGCTTGCATGGTCAGAGCACATCGACGTATCTGGCAATTGCCAATCGAG                                                 <  SRR4302544.1662501/94‑1 (MQ=60)

                                               GGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGAC                                               >  SRR4302544.2498089/1‑94 (MQ=60)

                                                GAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACG                                              <  SRR4302544.4153727/94‑1 (MQ=60)

                                                 AAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGC                                             >  SRR4302544.3112633/1‑94 (MQ=60)

                                                  AGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGAT                                            <  SRR4302544.2367005/94‑1 (MQ=60)

                                                   GAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATC                                           <  SRR4302544.504958/94‑1 (MQ=60)

                                                   GAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCGGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATC                                           <  SRR4302544.4617984/94‑1 (MQ=60)

                                                    AAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCA                                          <  SRR4302544.3856679/94‑1 (MQ=60)

                                                         CGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCG                                     <  SRR4302544.1722254/94‑1 (MQ=60)

                                                         CGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCG                                     <  SRR4302544.2406191/94‑1 (MQ=60)

                                                           CCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTG                                   >  SRR4302544.1064263/1‑94 (MQ=60)

                                                              TGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTG                                <  SRR4302544.275115/94‑1 (MQ=60)

                                                               GCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGG                               <  SRR4302544.536974/94‑1 (MQ=60)

                                                                CCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGC                              <  SRR4302544.2397533/94‑1 (MQ=60)

                                                                 CGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCG                             <  SRR4302544.2702878/94‑1 (MQ=60)

                                                                    CGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATT                          >  SRR4302544.2122700/1‑94 (MQ=60)

                                                                     GTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTA                         >  SRR4302544.3054321/1‑94 (MQ=60)

                                                                     GTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTA                         >  SRR4302544.4536423/1‑94 (MQ=60)

                                                                        AATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACG                      <  SRR4302544.1952024/94‑1 (MQ=60)

                                                                          TGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCTACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGAT                    <  SRR4302544.1327066/94‑1 (MQ=60)

                                                                          TGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGAT                    >  SRR4302544.4341799/1‑94 (MQ=60)

                                                                          TGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGAT                    <  SRR4302544.3553822/94‑1 (MQ=60)

                                                                          TGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGAT                    <  SRR4302544.1739809/94‑1 (MQ=60)

                                                                           GTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATA                   >  SRR4302544.1794646/1‑94 (MQ=60)

                                                                             CTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACC                 <  SRR4302544.796307/94‑1 (MQ=60)

                                                                             CTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACC                 >  SRR4302544.3487570/1‑94 (MQ=60)

                                                                               CCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCA               <  SRR4302544.2982268/94‑1 (MQ=60)

                                                                                CGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTCTCTGGCAATTGCCAATCGAGACGATCAAACCTGGCTGGCGATTAACCATACGCAT              >  SRR4302544.2506139/1‑94 (MQ=60)

                                                                                 GGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATC             >  SRR4302544.1639472/1‑94 (MQ=60)

                                                                                  GCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCT            <  SRR4302544.4015883/94‑1 (MQ=60)

                                                                                  GCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCT            <  SRR4302544.2786794/94‑1 (MQ=60)

                                                                                   CTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTG           <  SRR4302544.4544456/94‑1 (MQ=60)

                                                                                     GCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCT         >  SRR4302544.1317645/1‑94 (MQ=60)

                                                                                      CGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTG        >  SRR4302544.4281351/1‑94 (MQ=60)

                                                                                      CGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTG        >  SRR4302544.4281350/1‑94 (MQ=60)

                                                                                       GTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGG       >  SRR4302544.544994/1‑94 (MQ=60)

                                                                                       GTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGG       >  SRR4302544.256250/1‑94 (MQ=60)

                                                                                        TTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGA      >  SRR4302544.4129126/1‑94 (MQ=60)

                                                                                          CGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAAC    >  SRR4302544.1927873/1‑94 (MQ=60)

                                                                                          CGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAAC    >  SRR4302544.1541240/1‑94 (MQ=60)

                                                                                            CTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAG  <  SRR4302544.2413195/94‑1 (MQ=60)

CATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAG  >  NC_000913/2250050‑2250235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`