Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,254,317 T→C T347A (ACG→GCG)  yeiM ← putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,254,3170TC98.1% 490.4 / ‑2.8 157T347A (ACG→GCG) yeiMputative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (70/84);  total (72/85)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 162 total aligned reads displayed.

AAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGA  >  NC_000913/2254227‑2254405
                                                                                          |                                                                                        
aaaaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAgcgcgc                                                                                        >  1:4323992/1‑93 (MQ=255)
 aaaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCgcc                                                                                       <  1:1865161/93‑1 (MQ=255)
    tGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCgccgcc                                                                                    <  2:762400/93‑1 (MQ=255)
         cAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGttt                                                                               >  1:510632/1‑93 (MQ=255)
         cAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGttt                                                                               >  1:2184986/1‑93 (MQ=255)
          aaCGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTg                                                                              <  1:364984/93‑1 (MQ=255)
          aaCGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTg                                                                              >  2:4614864/1‑93 (MQ=255)
           aCGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGc                                                                             >  1:3132258/1‑93 (MQ=255)
           aCGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGc                                                                             >  1:2794148/1‑93 (MQ=255)
            cGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCa                                                                            <  1:4003297/93‑1 (MQ=255)
              acaacaCCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAgg                                                                          >  2:4481743/1‑93 (MQ=255)
              acaacaCCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAgg                                                                          <  1:4640324/93‑1 (MQ=255)
                aacaCCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTa                                                                        >  2:1282698/1‑93 (MQ=255)
                aacaCCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTa                                                                        >  2:3880158/1‑93 (MQ=255)
                 acacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAt                                                                       <  1:4284540/93‑1 (MQ=255)
                 acacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAt                                                                       <  1:2490595/93‑1 (MQ=255)
                 acacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAt                                                                       <  1:2762382/93‑1 (MQ=255)
                 acacCGATAGAACCAAAGTTAGCAAAACCACAAAGAGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAt                                                                       >  2:1091091/1‑93 (MQ=255)
                  cacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATg                                                                      <  2:2440540/93‑1 (MQ=255)
                  cacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATg                                                                      <  2:2802562/93‑1 (MQ=255)
                   acCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATgg                                                                     <  2:1942184/93‑1 (MQ=255)
                   acCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATgg                                                                     <  2:802273/93‑1 (MQ=255)
                   acCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATgg                                                                     <  1:1084873/93‑1 (MQ=255)
                    ccGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATggg                                                                    <  1:2820856/93‑1 (MQ=255)
                    ccGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATggg                                                                    <  1:4348885/93‑1 (MQ=255)
                    ccGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGCTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATggg                                                                    >  1:4274798/1‑93 (MQ=255)
                     cGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATgggg                                                                   <  2:2731037/93‑1 (MQ=255)
                      gATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATggggg                                                                  >  2:3490539/1‑92 (MQ=255)
                      gATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGga                                                                  >  2:3220215/1‑93 (MQ=255)
                       aTAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGgag                                                                 >  1:3525573/1‑93 (MQ=255)
                       aTAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGGTTGCAGGTATGGGgag                                                                 >  1:4222878/1‑93 (MQ=255)
                        tAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGgaga                                                                >  2:4540003/1‑93 (MQ=255)
                        tAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGgaga                                                                >  2:1716669/1‑93 (MQ=255)
                         aGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGaa                                                               >  1:4543411/1‑93 (MQ=255)
                          gAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGaaa                                                              <  1:1249645/93‑1 (MQ=255)
                            aCCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACt                                                            >  1:2912409/1‑93 (MQ=255)
                             ccAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTc                                                           <  1:3855502/93‑1 (MQ=255)
                             ccAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTc                                                           <  1:763936/93‑1 (MQ=255)
                              cAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCa                                                          <  1:1748492/93‑1 (MQ=255)
                              cAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCa                                                          >  1:905515/1‑93 (MQ=255)
                               aaaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAg                                                         <  2:2026877/93‑1 (MQ=255)
                                aaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCTTTTGCAGGTATGGGGAGAAACTCAgg                                                        >  1:2382603/1‑93 (MQ=255)
                                aaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAgg                                                        >  1:3058407/1‑93 (MQ=255)
                                  gTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTa                                                      <  1:1305206/93‑1 (MQ=255)
                                  gTTAGAAAAACCACAAAGCGCAAAGGAGATAAGCGCAATGGTTTTCACTTGCAGCGCGCCGCCCGTTTGCAGGTATGGTGAGATACTCAggga                                                      >  2:1644653/1‑91 (MQ=255)
                                   gtAGCAAAGCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTaa                                                     <  1:791184/92‑1 (MQ=255)
                                    tAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAg                                                    <  1:3389519/93‑1 (MQ=255)
                                       cAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcga                                                 <  2:73477/93‑1 (MQ=255)
                                           aCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGGGTGCCGCCCGTTTGCAGGAATGGGGGGAAACTCAGGAAAGCGACGaa                                             >  1:4495790/1‑93 (MQ=255)
                                           aCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGaa                                             >  1:1587881/1‑93 (MQ=255)
                                           aCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGaa                                             <  1:31074/93‑1 (MQ=255)
                                            ccACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAt                                            >  1:1060976/1‑93 (MQ=255)
                                              acaAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTc                                          <  1:3239670/93‑1 (MQ=255)
                                               caAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCg                                         <  2:3799962/93‑1 (MQ=255)
                                                aaaGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGt                                        >  1:64088/1‑93 (MQ=255)
                                                aaaGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGt                                        <  1:3967395/93‑1 (MQ=255)
                                                aaaGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGt                                        <  1:4045439/93‑1 (MQ=255)
                                                aaaGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCAGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGt                                        >  2:695914/1‑93 (MQ=255)
                                                 aaGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGtt                                       <  1:959576/93‑1 (MQ=255)
                                                  aGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTa                                      <  2:314669/93‑1 (MQ=255)
                                                    cgcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAAt                                    <  1:2908915/93‑1 (MQ=255)
                                                     gcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                   <  2:580168/93‑1 (MQ=255)
                                                     gcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                   >  2:4597266/1‑93 (MQ=255)
                                                     gcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                   <  1:1129018/93‑1 (MQ=255)
                                                     gcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                   <  2:2261400/93‑1 (MQ=255)
                                                       aaaGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGc                                 >  2:1451251/1‑93 (MQ=255)
                                                        aaGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGcc                                <  1:1533856/93‑1 (MQ=255)
                                                        aaGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGcc                                >  2:2486397/1‑93 (MQ=255)
                                                         aGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCa                               <  1:3745264/93‑1 (MQ=255)
                                                         aGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCa                               >  2:369408/1‑93 (MQ=255)
                                                         aGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCa                               <  2:3246020/93‑1 (MQ=255)
                                                         aGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCa                               <  2:3149916/93‑1 (MQ=255)
                                                           gagaTAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGt                             <  2:1370755/93‑1 (MQ=255)
                                                             gaTAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGttt                           >  1:4135444/1‑93 (MQ=255)
                                                             gaTAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGttt                           >  1:3269933/1‑93 (MQ=255)
                                                                aaTCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTg                        <  1:2335353/93‑1 (MQ=255)
                                                                 aTCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGc                       >  1:561911/1‑93 (MQ=255)
                                                                 aTCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGc                       >  2:2020886/1‑93 (MQ=255)
                                                                  tCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTTGTTAATCGCCAGTTTCTGcc                      <  2:3740544/93‑1 (MQ=255)
                                                                     cAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTNGTTAATCGCCAGTTTCTGCCCaa                   >  1:2466014/1‑93 (MQ=255)
                                                                     cAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCaa                   <  2:858210/93‑1 (MQ=255)
                                                                     cAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCaa                   >  2:1521030/1‑93 (MQ=255)
                                                                      aaTGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAAt                  <  2:2415158/93‑1 (MQ=255)
                                                                         ttttttCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAg               <  1:3382205/91‑1 (MQ=255)
                                                                         ggTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAg               <  1:4523379/93‑1 (MQ=255)
                                                                         ggTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAg               <  1:1263473/93‑1 (MQ=255)
                                                                          gTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAgg              >  2:4422231/1‑93 (MQ=255)
                                                                           ttctCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGc             <  2:217523/90‑1 (MQ=255)
                                                                            tttCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCt            >  2:3518050/1‑93 (MQ=255)
                                                                            tttCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCt            <  2:4041466/93‑1 (MQ=255)
                                                                            tttCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCt            <  2:447742/93‑1 (MQ=255)
                                                                            tttCACTGCCAGCCCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCt            <  2:2267840/93‑1 (MQ=255)
                                                                                aCTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAccc        >  2:1277030/1‑93 (MQ=255)
                                                                                aCTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAccc        <  1:1054336/93‑1 (MQ=255)
                                                                                aCTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAccc        >  2:3028062/1‑93 (MQ=255)
                                                                                 cTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCg       >  2:1651505/1‑93 (MQ=255)
                                                                                  ttCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGc      >  1:1918832/1‑93 (MQ=255)
                                                                                  ttCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGc      <  2:1780173/93‑1 (MQ=255)
                                                                                    ccAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCaa    <  2:3385559/93‑1 (MQ=255)
                                                                                      aGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGa  <  1:1803603/93‑1 (MQ=255)
                                                                                          |                                                                                        
AAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGA  >  NC_000913/2254227‑2254405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTGG  >  NC_000913/2254226‑2254409
                                                                                           |                                                                                            
GAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGC                                                                                            <  SRR4302544.1877395/94‑1 (MQ=60)
GAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGC                                                                                            >  SRR4302544.4368029/1‑94 (MQ=60)
 AAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCACAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCC                                                                                           >  SRR4302544.4566143/1‑94 (MQ=60)
  AAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCG                                                                                          <  SRR4302544.1883186/94‑1 (MQ=60)
         CCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTT                                                                                   >  SRR4302544.515671/1‑94 (MQ=60)
         CCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTT                                                                                   >  SRR4302544.2205652/1‑94 (MQ=60)
          CAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTG                                                                                  <  SRR4302544.4378521/94‑1 (MQ=60)
           AACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGC                                                                                 <  SRR4302544.368715/94‑1 (MQ=60)
           AACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGC                                                                                 >  SRR4302544.3162016/1‑94 (MQ=60)
           AACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGC                                                                                 >  SRR4302544.2821056/1‑94 (MQ=60)
           AACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGC                                                                                 >  SRR4302544.4703911/1‑94 (MQ=60)
             CGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAG                                                                               <  SRR4302544.4043137/94‑1 (MQ=60)
               ACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGT                                                                             <  SRR4302544.4686287/94‑1 (MQ=60)
                  ACACCGATAGAACCAAAGTTATCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATG                                                                          <  SRR4302544.406937/94‑1 (MQ=60)
                  ACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATG                                                                          <  SRR4302544.2788953/94‑1 (MQ=60)
                  ACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATG                                                                          <  SRR4302544.2514450/94‑1 (MQ=60)
                  ACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATG                                                                          <  SRR4302544.4328308/94‑1 (MQ=60)
                   CACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGG                                                                         <  SRR4302544.1683441/94‑1 (MQ=60)
                   CACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGG                                                                         >  SRR4302544.4352139/1‑94 (MQ=60)
                    ACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGG                                                                        <  SRR4302544.1095666/94‑1 (MQ=60)
                    ACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGCTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGG                                                                        >  SRR4302544.4318444/1‑94 (MQ=60)
                     CCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGG                                                                       >  SRR4302544.4270350/1‑94 (MQ=60)
                     CCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGG                                                                       <  SRR4302544.4393085/94‑1 (MQ=60)
                     CCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGG                                                                       <  SRR4302544.2848103/94‑1 (MQ=60)
                      CGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGA                                                                      >  SRR4302544.350777/1‑94 (MQ=60)
                       GATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAG                                                                     >  SRR4302544.2666891/1‑94 (MQ=60)
                       GATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAG                                                                     >  SRR4302544.3559262/1‑94 (MQ=60)
                       GATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGGTTGCAGGTATGGGGAG                                                                     >  SRR4302544.4265748/1‑94 (MQ=60)
                         TAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAA                                                                   >  SRR4302544.4588806/1‑94 (MQ=60)
                           GAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAAC                                                                 <  SRR4302544.1261944/94‑1 (MQ=60)
                            AACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCCGGTATGGGGAGAAACT                                                                >  SRR4302544.107653/1‑94 (MQ=60)
                            AACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACT                                                                >  SRR4302544.2940427/1‑94 (MQ=60)
                              CCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCA                                                              <  SRR4302544.3893781/94‑1 (MQ=60)
                              CCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCA                                                              >  SRR4302544.914475/1‑94 (MQ=60)
                              CCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCA                                                              <  SRR4302544.771382/94‑1 (MQ=60)
                              CCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCA                                                              <  SRR4302544.2875884/94‑1 (MQ=60)
                               CAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAG                                                             >  SRR4302544.2511514/1‑94 (MQ=60)
                               CAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAG                                                             <  SRR4302544.1765609/94‑1 (MQ=60)
                                AAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCTTTTGCAGGTATGGGGAGAAACTCAGG                                                            >  SRR4302544.2405163/1‑94 (MQ=60)
                                AAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGG                                                            <  SRR4302544.2032881/94‑1 (MQ=60)
                                AAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGG                                                            >  SRR4302544.3087596/1‑94 (MQ=60)
                                AAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGG                                                            <  SRR4302544.627840/94‑1 (MQ=60)
                                 AAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGT                                                           >  SRR4302544.124539/1‑94 (MQ=60)
                                   GTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAA                                                         <  SRR4302544.1318038/94‑1 (MQ=60)
                                    GTAGCAAAGCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAG                                                        <  SRR4302544.798842/94‑1 (MQ=60)
                                     TAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGC                                                       <  SRR4302544.3421910/94‑1 (MQ=60)
                                        CAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGAC                                                    <  SRR4302544.1271417/94‑1 (MQ=60)
                                        CAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGAC                                                    >  SRR4302544.1923236/1‑94 (MQ=60)
                                        CAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGAC                                                    >  SRR4302544.3582400/1‑94 (MQ=60)
                                           AACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGGGTGCCGCCCGTTTGCAGGAATGGGGGGAAACTCAGGAAAGCGACGAA                                                 >  SRR4302544.4540913/1‑94 (MQ=60)
                                           AACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAA                                                 >  SRR4302544.1603551/1‑94 (MQ=60)
                                            ACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAT                                                <  SRR4302544.31370/94‑1 (MQ=60)
                                            ACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAT                                                >  SRR4302544.1071514/1‑94 (MQ=60)
                                              CACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTC                                              <  SRR4302544.4211942/94‑1 (MQ=60)
                                               ACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCG                                             <  SRR4302544.3270679/94‑1 (MQ=60)
                                               ACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCG                                             <  SRR4302544.3270680/94‑1 (MQ=60)
                                               ACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCG                                             <  SRR4302544.1777047/94‑1 (MQ=60)
                                                CAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGT                                            >  SRR4302544.64734/1‑94 (MQ=60)
                                                 AAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTT                                           <  SRR4302544.4006858/94‑1 (MQ=60)
                                                 AAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTT                                           <  SRR4302544.4085734/94‑1 (MQ=60)
                                                  AAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTA                                          <  SRR4302544.968999/94‑1 (MQ=60)
                                                  AAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTA                                          >  SRR4302544.2558276/1‑94 (MQ=60)
                                                     CGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATC                                       <  SRR4302544.2936904/94‑1 (MQ=60)
                                                      GCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCG                                      <  SRR4302544.1140274/94‑1 (MQ=60)
                                                         AAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCA                                   >  SRR4302544.687581/1‑94 (MQ=60)
                                                         AAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCA                                   <  SRR4302544.1549090/94‑1 (MQ=60)
                                                          AGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAG                                  <  SRR4302544.3782473/94‑1 (MQ=60)
                                                           GGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGT                                 <  SRR4302544.386459/94‑1 (MQ=60)
                                                            GAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTT                                >  SRR4302544.2991393/1‑94 (MQ=60)
                                                             AGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTT                               >  SRR4302544.3301124/1‑94 (MQ=60)
                                                             AGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTT                               >  SRR4302544.4176408/1‑94 (MQ=60)
                                                                TAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTG                            >  SRR4302544.3894861/1‑94 (MQ=60)
                                                                 AATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGC                           <  SRR4302544.2357489/94‑1 (MQ=60)
                                                                 AATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGC                           >  SRR4302544.567407/1‑94 (MQ=60)
                                                                   TCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCC                         <  SRR4302544.2831947/94‑1 (MQ=60)
                                                                     GCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTNGTTAATCGCCAGTTTCTGCCCAA                       >  SRR4302544.2489570/1‑94 (MQ=60)
                                                                      CAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAAT                      <  SRR4302544.1477420/94‑1 (MQ=60)
                                                                      CAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAAT                      >  SRR4302544.873472/1‑94 (MQ=60)
                                                                       AATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATC                     <  SRR4302544.4680041/94‑1 (MQ=60)
                                                                        ATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCA                    >  SRR4302544.3139671/1‑94 (MQ=60)
                                                                          ttTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGG                  <  SRR4302544.3414504/92‑1 (MQ=60)
                                                                          GGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGG                  <  SRR4302544.4568656/94‑1 (MQ=60)
                                                                          GGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGG                  <  SRR4302544.1275902/94‑1 (MQ=60)
                                                                              TTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAC              <  SRR4302544.2385295/94‑1 (MQ=60)
                                                                                 ACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCG           <  SRR4302544.1064789/94‑1 (MQ=60)
                                                                                 ACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCG           <  SRR4302544.3359856/94‑1 (MQ=60)
                                                                                  CTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGC          >  SRR4302544.1937373/1‑94 (MQ=60)
                                                                                       AGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAT     <  SRR4302544.1821223/94‑1 (MQ=60)
                                                                                          GCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTGG  >  SRR4302544.1321463/1‑94 (MQ=60)
                                                                                          GCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTGG  <  SRR4302544.3105490/94‑1 (MQ=60)
                                                                                           |                                                                                            
GAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTGG  >  NC_000913/2254226‑2254409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑