Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,250,142	T→C	R141R (CGT→CGC)	yeiI →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,250,142	0	T	C	100.0%	767.1 / NA	235	R141R (CGT→CGC)	yeiI	putative kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (103/132); total (103/132)

Only 100 of 248 total aligned reads displayed.

TTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGA  >  NC_000913/2250052‑2250231

tttGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCAGGCGTGAATGTCTCCGGCTGCGTTCGctt                                                                                         >  2:882591/1‑93 (MQ=255)

 ttGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTg                                                                                        <  1:715095/93‑1 (MQ=255)

    cTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCAt                                                                                     >  1:633602/1‑93 (MQ=255)

    cTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCAt                                                                                     >  1:3139055/1‑93 (MQ=255)

    cTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCAt                                                                                     >  1:5685497/1‑93 (MQ=255)

    cTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCAt                                                                                     <  1:2316301/93‑1 (MQ=255)

      ttCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATgg                                                                                   <  1:4927159/93‑1 (MQ=255)

       tCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGt                                                                                  <  1:4667044/93‑1 (MQ=255)

          gTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaa                                                                               <  2:3723683/93‑1 (MQ=255)

          gTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaa                                                                               >  2:5235338/1‑93 (MQ=255)

           tGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaaa                                                                              <  2:3170603/93‑1 (MQ=255)

           tGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaaa                                                                              <  2:1901223/93‑1 (MQ=255)

           tGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaaa                                                                              <  2:6604568/93‑1 (MQ=255)

           tGATTGGCAATGACTTTTACGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCaaa                                                                              >  1:6516809/1‑93 (MQ=255)

            gATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCCTGGTCAAAg                                                                             >  1:1453084/1‑93 (MQ=255)

            gATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAg                                                                             >  2:2233548/1‑93 (MQ=255)

             aTTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGc                                                                            <  1:3065969/93‑1 (MQ=255)

               tGGCCATCACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGcac                                                                          <  2:2377554/93‑1 (MQ=255)

                ggCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGcaca                                                                         <  1:2881586/93‑1 (MQ=255)

                ggCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGcaca                                                                         >  1:4253969/1‑93 (MQ=255)

                  cGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATc                                                                       >  1:5299901/1‑93 (MQ=255)

                  cGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATc                                                                       <  2:5314819/93‑1 (MQ=255)

                  cGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATc                                                                       <  2:6103687/93‑1 (MQ=255)

                   gATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCg                                                                      <  2:4069103/93‑1 (MQ=255)

                    aTGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGa                                                                     <  1:6213771/93‑1 (MQ=255)

                    aTGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGa                                                                     <  2:3959054/93‑1 (MQ=255)

                    aTGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGa                                                                     <  2:5273299/93‑1 (MQ=255)

                       aCTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGt                                                                  >  2:3454294/1‑93 (MQ=255)

                       aCTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGt                                                                  >  2:1634335/1‑93 (MQ=255)

                       aCTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGt                                                                  <  1:2577624/93‑1 (MQ=255)

                        cTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTa                                                                 <  1:1316985/93‑1 (MQ=255)

                         ttttATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTAt                                                                <  1:4208844/93‑1 (MQ=255)

                          tttATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATc                                                               <  2:6472835/93‑1 (MQ=255)

                           ttATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCt                                                              <  2:739505/93‑1 (MQ=255)

                              tGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGc                                                           >  1:1681798/1‑93 (MQ=255)

                              tGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGc                                                           >  1:5531857/1‑93 (MQ=255)

                                gCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCaa                                                         >  1:125312/1‑93 (MQ=255)

                                 cGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAAt                                                        <  1:1218581/93‑1 (MQ=255)

                                  gAAATGCTCCTGGAAGAAACGCGCCGTGCCGGAGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCCAACCACATCGACGTATATGGCGAtt                                                       >  2:1854253/1‑93 (MQ=255)

                                   aaaTGCTCGTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTg                                                      <  2:4977101/93‑1 (MQ=255)

                                   aaaTGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTg                                                      <  2:1448777/93‑1 (MQ=255)

                                    aaTGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGc                                                     <  1:979951/93‑1 (MQ=255)

                                     aTGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGcc                                                    <  1:523246/93‑1 (MQ=255)

                                      tGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCGTGGTCAAAGCACATCGACGTATCTGGCAATTGCCa                                                   >  2:3085268/1‑93 (MQ=255)

                                      tGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCa                                                   >  1:6367474/1‑93 (MQ=255)

                                      tGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCa                                                   <  1:204283/93‑1 (MQ=255)

                                        cTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAAt                                                 <  2:366037/93‑1 (MQ=255)

                                          ccTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCg                                               <  2:6050192/93‑1 (MQ=255)

                                           cTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCga                                              <  1:6027421/93‑1 (MQ=255)

                                           cTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCga                                              <  2:2634025/93‑1 (MQ=255)

                                            tGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCgag                                             >  2:2167576/1‑93 (MQ=255)

                                             ggAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCgaga                                            >  2:1217172/1‑93 (MQ=255)

                                             ggAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCgaga                                            <  2:6654935/93‑1 (MQ=255)

                                             ggAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCgaga                                            <  2:3949596/93‑1 (MQ=255)

                                              gaagaaACGCGCCGTGCCGGCGTGAATGTCTCCGTCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGAc                                           <  2:594534/93‑1 (MQ=255)

                                              gaagaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGAc                                           <  2:3887989/93‑1 (MQ=255)

                                              gaagaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGAc                                           >  2:6219369/1‑93 (MQ=255)

                                              gaagaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGAc                                           <  1:1842638/93‑1 (MQ=255)

                                                 gaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGAt                                        <  2:4525090/93‑1 (MQ=255)

                                                 gaaACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGAt                                        >  1:4142728/1‑93 (MQ=255)

                                                  aaaCGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATc                                       <  2:2696403/93‑1 (MQ=255)

                                                   aaCGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCa                                      >  2:1769837/1‑93 (MQ=255)

                                                   aaCGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCa                                      >  2:4028018/1‑93 (MQ=255)

                                                    aagcgcCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCaa                                     >  1:3991400/3‑93 (MQ=255)

                                                    aCGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCaa                                     >  2:2840679/1‑93 (MQ=255)

                                                    aCGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCaa                                     <  1:372210/93‑1 (MQ=255)

                                                     cgcgCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCaaa                                    >  1:2701137/1‑93 (MQ=255)

                                                       cgcCGTGCCGCCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAAcc                                  <  2:426436/93‑1 (MQ=255)

                                                        gcCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCg                                 >  2:1080517/1‑93 (MQ=255)

                                                           gttccGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGc                              <  2:1248853/90‑1 (MQ=255)

                                                            tGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCt                             <  1:3258909/93‑1 (MQ=255)

                                                             gCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTg                            <  2:3277129/93‑1 (MQ=255)

                                                               cGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGc                          <  1:343453/93‑1 (MQ=255)

                                                                 gCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGa                        <  1:2468696/93‑1 (MQ=255)

                                                                 gCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGa                        <  2:1056811/93‑1 (MQ=255)

                                                                  cGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGAt                       <  1:2205125/93‑1 (MQ=255)

                                                                   gTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGAtt                      >  1:1010011/1‑93 (MQ=255)

                                                                   gTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGAtt                      >  1:6090860/1‑93 (MQ=255)

                                                                   gTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGAtt                      <  2:250953/93‑1 (MQ=255)

                                                                    tGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTa                     >  2:3519526/1‑93 (MQ=255)

                                                                    tGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTa                     >  2:4856124/1‑93 (MQ=255)

                                                                      aaTGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAAc                   <  1:3816256/93‑1 (MQ=255)

                                                                      aaTGTCTCCGGCTGCGCTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTAGCGATTAAc                   >  1:4504176/1‑93 (MQ=255)

                                                                       aTGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACg                  >  2:1010730/1‑93 (MQ=255)

                                                                        tGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGa                 <  1:5593639/93‑1 (MQ=255)

                                                                         gTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGAt                >  2:5802204/1‑93 (MQ=255)

                                                                         gTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGAt                <  1:4817482/93‑1 (MQ=255)

                                                                           ctcCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAc              >  1:1562666/1‑93 (MQ=255)

                                                                            tcCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAcc             <  2:3017908/93‑1 (MQ=255)

                                                                             ccGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAccc            <  2:5834454/93‑1 (MQ=255)

                                                                             ccGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAccc            <  1:3973539/93‑1 (MQ=255)

                                                                             ccGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAccc            <  2:2814633/93‑1 (MQ=255)

                                                                             ccGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAccc            <  2:4873486/93‑1 (MQ=255)

                                                                                gCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATc         >  2:5715374/1‑93 (MQ=255)

                                                                                gCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATc         <  2:1710787/93‑1 (MQ=255)

                                                                                gCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATc         >  1:5783193/1‑93 (MQ=255)

                                                                                   gCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATctgc      <  1:3530410/93‑1 (MQ=255)

                                                                                   gCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATctgc      <  1:6253446/93‑1 (MQ=255)

                                                                                     gttcgctTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATctgctg    >  2:2048675/1‑93 (MQ=255)

                                                                                     gttcgctTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATctgctg    <  1:210898/93‑1 (MQ=255)

TTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGA  >  NC_000913/2250052‑2250231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAG  >  NC_000913/2250049‑2250235

CCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGC                                                                                               >  SRR4302536.6466304/1‑94 (MQ=60)

CCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGC                                                                                               >  SRR4302536.4469904/1‑94 (MQ=60)

 CATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCT                                                                                              >  SRR4302536.4789349/1‑94 (MQ=60)

 CATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCT                                                                                              <  SRR4302536.1716534/94‑1 (MQ=60)

 CATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCT                                                                                              >  SRR4302536.2953662/1‑94 (MQ=60)

 CATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGGTCGCT                                                                                              >  SRR4302536.5938853/1‑94 (MQ=60)

  tttctcttgacggtcttgctgataactttattgtcaatctgctcctataacaacCGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGATT                                                                                             <  SRR4302536.5785484/40‑1 (MQ=60)

  attgtCTTTCAGTGATTGGCGATGACTTTTATGGCTAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTT                                                                                             <  SRR4302536.130578/89‑1 (MQ=60)

  ATTTGCTTTCAGTGCTTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTT                                                                                             >  SRR4302536.6627548/1‑94 (MQ=60)

    TTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGC                                                                                           >  SRR4302536.3948480/1‑94 (MQ=60)

    TTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGC                                                                                           <  SRR4302536.736006/94‑1 (MQ=60)

    TTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGC                                                                                           >  SRR4302536.6583010/1‑94 (MQ=60)

     TGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCA                                                                                          >  SRR4302536.4364924/1‑94 (MQ=60)

      GCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCAT                                                                                         <  SRR4302536.4878079/94‑1 (MQ=60)

      GCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCAT                                                                                         >  SRR4302536.5854555/1‑94 (MQ=60)

      GCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCAT                                                                                         >  SRR4302536.3230933/1‑94 (MQ=60)

      GCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCAT                                                                                         >  SRR4302536.652341/1‑94 (MQ=60)

       CTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATG                                                                                        <  SRR4302536.2386599/94‑1 (MQ=60)

         TTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGT                                                                                      <  SRR4302536.5075200/94‑1 (MQ=60)

         TTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGGTCGCTTGCATGGT                                                                                      >  SRR4302536.3039204/1‑94 (MQ=60)

          TCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTC                                                                                     <  SRR4302536.4807369/94‑1 (MQ=60)

           CAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGGCA                                                                                    >  SRR4302536.6527200/1‑94 (MQ=60)

             GTGATTGGCAATGACTTTTACGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAA                                                                                  >  SRR4302536.6710974/1‑94 (MQ=60)

              TGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCCTGGTCAAAG                                                                                 >  SRR4302536.1496124/1‑94 (MQ=60)

              TGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAG                                                                                 <  SRR4302536.714347/94‑1 (MQ=60)

               GATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGC                                                                                >  SRR4302536.5766428/1‑94 (MQ=60)

                ATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCA                                                                               <  SRR4302536.3155859/94‑1 (MQ=60)

                  TGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACA                                                                             >  SRR4302536.4380704/1‑94 (MQ=60)

                   GGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACAT                                                                            <  SRR4302536.4571359/94‑1 (MQ=60)

                   GGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACAT                                                                            <  SRR4302536.2966632/94‑1 (MQ=60)

                   GGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACAT                                                                            >  SRR4302536.2526814/1‑94 (MQ=60)

                    GCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATC                                                                           >  SRR4302536.5457966/1‑94 (MQ=60)

                    GCGATGACTTATAGGGCAAAATGCTCCTGGAAGAAGCGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATC                                                                           <  SRR4302536.3882350/94‑1 (MQ=60)

                      GATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGGCAAAGCACATCGA                                                                         >  SRR4302536.2713743/1‑94 (MQ=60)

                       ATGGCTATTATGCCGAAATGATCCTGAAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGAC                                                                        <  SRR4302536.5855653/94‑1 (MQ=60)

                       ATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGAC                                                                        <  SRR4302536.6398904/94‑1 (MQ=60)

                       ATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGAC                                                                        >  SRR4302536.2839708/1‑94 (MQ=60)

                          ACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTA                                                                     <  SRR4302536.1486304/94‑1 (MQ=60)

                          ACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTA                                                                     <  SRR4302536.2655116/94‑1 (MQ=60)

                           CTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTAT                                                                    <  SRR4302536.1356300/94‑1 (MQ=60)

                           CTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTAT                                                                    <  SRR4302536.1950715/94‑1 (MQ=60)

                           CTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTAT                                                                    >  SRR4302536.3563965/1‑94 (MQ=60)

                            TTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATC                                                                   <  SRR4302536.3184165/94‑1 (MQ=60)

                            TTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATC                                                                   <  SRR4302536.4333948/94‑1 (MQ=60)

                             TTTATGGCGAAATGCTCCTGGAAGAAGCGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCT                                                                  <  SRR4302536.4102028/94‑1 (MQ=60)

                             TTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCT                                                                  <  SRR4302536.1500711/94‑1 (MQ=60)

                              TTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTG                                                                 >  SRR4302536.2755563/1‑94 (MQ=60)

                              TTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTG                                                                 <  SRR4302536.1347744/94‑1 (MQ=60)

                                ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGC                                                               >  SRR4302536.5696761/1‑94 (MQ=60)

                                ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGC                                                               >  SRR4302536.1730536/1‑94 (MQ=60)

                                ATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGC                                                               >  SRR4302536.2319480/1‑94 (MQ=60)

                                 TGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCA                                                              >  SRR4302536.928510/1‑94 (MQ=60)

                                  GGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAA                                                             >  SRR4302536.128616/1‑94 (MQ=60)

                                    CGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATT                                                           <  SRR4302536.6015137/94‑1 (MQ=60)

                                    CGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATT                                                           <  SRR4302536.1255180/94‑1 (MQ=60)

                                     GAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTG                                                          >  SRR4302536.6026736/1‑94 (MQ=60)

                                     GAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTG                                                          <  SRR4302536.6411070/94‑1 (MQ=60)

                                      AAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGC                                                         >  SRR4302536.6489549/1‑94 (MQ=60)

                                       AATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCC                                                        <  SRR4302536.553908/94‑1 (MQ=60)

                                       AATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCC                                                        <  SRR4302536.1008880/94‑1 (MQ=60)

                                       AATGCGCCTGGAAGAACCCCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCC                                                        <  SRR4302536.1124067/94‑1 (MQ=60)

                                        ATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCA                                                       >  SRR4302536.6557219/1‑94 (MQ=60)

                                        ATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCA                                                       <  SRR4302536.536647/94‑1 (MQ=60)

                                         TGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAA                                                      <  SRR4302536.209659/94‑1 (MQ=60)

                                          GCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAAT                                                     <  SRR4302536.472640/94‑1 (MQ=60)

                                          GCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAAT                                                     <  SRR4302536.5788678/94‑1 (MQ=60)

                                           CTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATC                                                    >  SRR4302536.5432743/1‑94 (MQ=60)

                                           CTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATC                                                    >  SRR4302536.912248/1‑94 (MQ=60)

                                             CCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGA                                                  <  SRR4302536.4994649/94‑1 (MQ=60)

                                             CCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGA                                                  >  SRR4302536.3377427/1‑94 (MQ=60)

                                             CCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGA                                                  >  SRR4302536.3654252/1‑94 (MQ=60)

                                             CCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGA                                                  >  SRR4302536.1259259/1‑94 (MQ=60)

                                             CCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGGATCTGGCAATTGCCAATCGA                                                  >  SRR4302536.639210/1‑94 (MQ=60)

                                              CTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAG                                                 >  SRR4302536.5205915/1‑94 (MQ=60)

                                              CTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAG                                                 <  SRR4302536.6207130/94‑1 (MQ=60)

                                               TGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGGTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGGCAATCGAGA                                                >  SRR4302536.3220292/1‑94 (MQ=60)

                                               TGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGCTCGCTTGCATGGTCATAGCACATCGACGTATCTGGCAATTGCCACTCGAGA                                                >  SRR4302536.6519427/1‑94 (MQ=60)

                                               TGGAAGAAACGCGCCGGGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCAAGGTCATAGGACATCGACGTATCTGGCCATTGCCACTCGAGA                                                >  SRR4302536.3200954/1‑94 (MQ=60)

                                                GGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGAC                                               <  SRR4302536.2559956/94‑1 (MQ=60)

                                                 GAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACG                                              <  SRR4302536.1898167/94‑1 (MQ=60)

                                                  AAGAAACGCGCCGTGCCGGCGTGAAAGTCTCCGGCTACGTTCACCTGCATGGTCAAAGGACATCtactgatctggggatttctaatcgacacga                                             >  SRR4302536.1152151/1‑64 (MQ=32)

                                                   AGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGAT                                            <  SRR4302536.4187343/94‑1 (MQ=60)

                                                   AGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGAT                                            >  SRR4302536.4265609/1‑94 (MQ=60)

                                                   AGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGAT                                            <  SRR4302536.4187342/94‑1 (MQ=60)

                                                   AGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGAT                                            <  SRR4302536.1334272/94‑1 (MQ=60)

                                                     AAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCA                                          >  SRR4302536.2130226/1‑94 (MQ=60)

                                                     AAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCA                                          >  SRR4302536.3482265/1‑94 (MQ=60)

                                                     AAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCA                                          <  SRR4302536.568264/94‑1 (MQ=60)

                                                     AAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCCACGTATCTTGCAATTGTCCATCGAGACGATCA                                          >  SRR4302536.4736092/1‑94 (MQ=60)

                                                      AACGCGCGGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAA                                         <  SRR4302536.1809083/94‑1 (MQ=60)

                                                      AACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAA                                         <  SRR4302536.6313947/94‑1 (MQ=60)

                                                      AAAGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAA                                         >  SRR4302536.4109858/1‑94 (MQ=60)

                                                       ACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAA                                        >  SRR4302536.2781730/1‑94 (MQ=60)

                                                       ACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAA                                        <  SRR4302536.382028/94‑1 (MQ=60)

                                                        CGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAAC                                       >  SRR4302536.3994450/1‑94 (MQ=60)

                                                        CGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAAC                                       >  SRR4302536.3940009/1‑94 (MQ=60)

                                                         GCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACC                                      <  SRR4302536.623065/94‑1 (MQ=60)

                                                         GCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACC                                      <  SRR4302536.1517809/94‑1 (MQ=60)

                                                          CGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCG                                     >  SRR4302536.4328077/1‑94 (MQ=60)

                                                               TGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTG                                <  SRR4302536.4944111/94‑1 (MQ=60)

                                                               TGCCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTG                                <  SRR4302536.3354439/94‑1 (MQ=60)

                                                                 CCGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGC                              >  SRR4302536.1616555/1‑94 (MQ=60)

                                                                  CGGCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCG                             <  SRR4302536.352561/94‑1 (MQ=60)

                                                                    GCGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGAT                           <  SRR4302536.2543226/94‑1 (MQ=60)

                                                                     CGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATT                          >  SRR4302536.6272497/1‑94 (MQ=60)

                                                                     CGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATT                          >  SRR4302536.1039802/1‑94 (MQ=60)

                                                                     CGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATT                          <  SRR4302536.2271704/94‑1 (MQ=60)

                                                                     CGTGAATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATT                          >  SRR4302536.2559137/1‑94 (MQ=60)

                                                                        GAATGTCTCCGGCTGCGCTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTAGCGATTAAC                       >  SRR4302536.4639337/1‑94 (MQ=60)

                                                                         AATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACG                      >  SRR4302536.2846253/1‑94 (MQ=60)

                                                                         AATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACG                      >  SRR4302536.3280395/1‑94 (MQ=60)

                                                                         AATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACG                      <  SRR4302536.3929986/94‑1 (MQ=60)

                                                                          ATGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGA                     <  SRR4302536.5903213/94‑1 (MQ=60)

                                                                           TGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGAT                    <  SRR4302536.5759943/94‑1 (MQ=60)

                                                                           TGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGAT                    <  SRR4302536.5281361/94‑1 (MQ=60)

                                                                           TGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGAT                    <  SRR4302536.4487072/94‑1 (MQ=60)

                                                                           TGTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGAT                    >  SRR4302536.3713626/1‑94 (MQ=60)

                                                                            GTCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATA                   <  SRR4302536.4962452/94‑1 (MQ=60)

                                                                             TCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAC                  >  SRR4302536.1608551/1‑94 (MQ=60)

                                                                             TCTCCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATAC                  >  SRR4302536.2251157/1‑94 (MQ=60)

                                                                                CCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCA               <  SRR4302536.2146822/94‑1 (MQ=60)

                                                                                CCGGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCA               <  SRR4302536.4091482/94‑1 (MQ=60)

                                                                                  GGCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATC             >  SRR4302536.5955318/1‑94 (MQ=60)

                                                                                   GCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATCCCCATCT            <  SRR4302536.1806073/94‑1 (MQ=60)

                                                                                   GCTGCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCT            <  SRR4302536.2330150/94‑1 (MQ=60)

                                                                                      GCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCT         <  SRR4302536.6439751/94‑1 (MQ=60)

                                                                                      GCGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCT         <  SRR4302536.3635901/94‑1 (MQ=60)

                                                                                       CGTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGGGCTGGCGATTAACGATAACCATCTGCTG        >  SRR4302536.5500004/1‑94 (MQ=60)

                                                                                        GTTCGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGG       <  SRR4302536.216489/94‑1 (MQ=60)

                                                                                           CGCTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGGGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAAC    <  SRR4302536.666833/94‑1 (MQ=60)

                                                                                            GCTTGCACGGTCAAAGCACATCGACCTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACA   <  SRR4302536.397011/94‑1 (MQ=60)

                                                                                             CTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAG  >  SRR4302536.4421502/1‑94 (MQ=60)

                                                                                             CTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAG  >  SRR4302536.3586318/1‑94 (MQ=60)

                                                                                             CTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAG  >  SRR4302536.6888087/1‑94 (MQ=60)

                                                                                             CTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAG  <  SRR4302536.5848128/94‑1 (MQ=60)

                                                                                             CTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAAAAG  >  SRR4302536.5527932/1‑94 (MQ=60)

                                                                                             CTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCCGCTGGAACAG  >  SRR4302536.3926341/1‑94 (MQ=60)

CCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACATCGACGTATCTGGCAATTGCCAATCGAGACGATCAAACCGTGCTGGCGATTAACGATACCCATCTGCTGGAACAG  >  NC_000913/2250049‑2250235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`