Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,261,454	G→A	A21V (GCG→GTG)	fruB ←	PTS system, fructose‑specific IIA/fpr component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,261,454	0	G	A	99.4%	515.2 / NA	155	A21V (GCG→GTG)	fruB	PTS system, fructose‑specific IIA/fpr component
Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (95/57); minor base C (0/1); total (95/60)
Fisher's exact test for biased strand distribution p-value = 3.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

AGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACG  >  NC_000913/2261365‑2261543

 ggTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACctc                                                                                       <  1:1262693/93‑1 (MQ=255)

  gTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACctct                                                                                      >  1:1174265/1‑93 (MQ=255)

   tttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtctt                                                                                     >  2:178416/1‑93 (MQ=255)

     tGCTGTTCGTGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtcttct                                                                                   <  2:2681631/93‑1 (MQ=255)

     tGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtcttct                                                                                   >  2:1093167/1‑93 (MQ=255)

     tGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtcttct                                                                                   <  2:230477/93‑1 (MQ=255)

     tGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtcttct                                                                                   >  2:3914292/1‑93 (MQ=255)

      gCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTcttctt                                                                                  >  1:2607203/1‑93 (MQ=255)

       cTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCttt                                                                                 <  1:3174713/93‑1 (MQ=255)

        tGTTCGCGCGCCAACATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTg                                                                                >  1:2966704/1‑93 (MQ=255)

           tCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGtct                                                                             <  2:3887025/93‑1 (MQ=255)

            cgcgcgCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGtctc                                                                            <  2:3698967/93‑1 (MQ=255)

            cgcgcgCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGtctc                                                                            <  2:1333504/93‑1 (MQ=255)

             gcgcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTcc                                                                           <  2:4318577/93‑1 (MQ=255)

               gcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCgg                                                                         >  1:1897727/1‑93 (MQ=255)

                 gcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGcc                                                                       <  2:3251088/93‑1 (MQ=255)

                  ccAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCt                                                                      >  1:4081093/1‑93 (MQ=255)

                    aGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCttt                                                                    >  2:1674686/1‑93 (MQ=255)

                    aGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCttt                                                                    <  1:1409491/93‑1 (MQ=255)

                    aGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCttt                                                                    >  1:3027955/1‑93 (MQ=255)

                    aGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCttt                                                                    >  1:3913758/1‑93 (MQ=255)

                    aGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCCCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCttt                                                                    >  2:1674685/1‑93 (MQ=255)

                     gCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCtttt                                                                   >  1:2969581/1‑93 (MQ=255)

                      cATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTc                                                                  <  2:3220189/93‑1 (MQ=255)

                       aTGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCg                                                                 >  1:1620792/1‑93 (MQ=255)

                           cATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCg                                                             >  1:347385/1‑93 (MQ=255)

                            aTTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgg                                                            >  2:2785235/1‑93 (MQ=255)

                             ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgga                                                           >  1:2124147/1‑93 (MQ=255)

                              tGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAACCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggat                                                          <  2:4694930/93‑1 (MQ=255)

                                aCGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatgg                                                        >  2:4450240/1‑93 (MQ=255)

                                 cGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatgga                                                       >  1:3294507/1‑93 (MQ=255)

                                 cGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatgga                                                       <  2:3428925/93‑1 (MQ=255)

                                  gTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatggat                                                      >  2:2141923/1‑93 (MQ=255)

                                   tAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGgatggatg                                                     <  1:640305/93‑1 (MQ=255)

                                   tAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTACGGCCTTTTCGCCCGgatggatg                                                     >  1:2463652/1‑93 (MQ=255)

                                    aGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGt                                                    >  1:2550566/1‑93 (MQ=255)

                                     gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTc                                                   >  1:3889904/1‑93 (MQ=255)

                                      ccTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTcc                                                  <  1:86439/93‑1 (MQ=255)

                                      ccTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTcc                                                  <  2:2111696/93‑1 (MQ=255)

                                        ttCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTg                                                <  2:2895530/93‑1 (MQ=255)

                                        ttCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTg                                                >  2:4595617/1‑93 (MQ=255)

                                        ttCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTg                                                >  1:3650370/1‑93 (MQ=255)

                                        ttCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTg                                                <  1:1848819/93‑1 (MQ=255)

                                         tCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGt                                               >  2:1387463/1‑93 (MQ=255)

                                           tGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAc                                             >  2:2457868/1‑93 (MQ=255)

                                           tGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAc                                             >  2:2054426/1‑93 (MQ=255)

                                           tGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAc                                             >  1:1615109/1‑93 (MQ=255)

                                           tGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAc                                             <  1:4581968/93‑1 (MQ=255)

                                           tGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAc                                             >  1:606777/1‑93 (MQ=255)

                                            gCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACg                                            >  2:1149311/1‑93 (MQ=255)

                                             cTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACgg                                           <  1:2115971/93‑1 (MQ=255)

                                              tACATTACCGACCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGa                                          <  1:1970892/93‑1 (MQ=255)

                                                 aTTACCGGCCTGCCCCAGCTCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATaa                                       <  2:3861627/93‑1 (MQ=255)

                                                  ttACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAc                                      >  1:4269980/1‑93 (MQ=255)

                                                  ttACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAc                                      >  1:4269981/1‑93 (MQ=255)

                                                  ttACCGGCCTGCACCAGCGCCGCAGCCACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAc                                      <  1:1039714/93‑1 (MQ=255)

                                                    aCCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTg                                    <  2:2905256/93‑1 (MQ=255)

                                                     ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTgg                                   <  2:2950419/93‑1 (MQ=255)

                                                       ggCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGaa                                 >  1:1043831/1‑93 (MQ=255)

                                                        gCCTGCCCCAGCGCCGCAGCGACCTGGCGAATCACCTCTAATTTGTCTCCGGCCTTTTCGGTCGGATGGATGTCCTGTACGGATAACTGGAAc                                <  2:1475720/93‑1 (MQ=255)

                                                          cTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACAt                              >  2:4673657/1‑93 (MQ=255)

                                                           tGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATa                             >  1:2389214/1‑93 (MQ=255)

                                                            gCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAg                            <  2:3849530/93‑1 (MQ=255)

                                                             cACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGt                           <  2:2854715/93‑1 (MQ=255)

                                                             cACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGt                           <  1:1348646/93‑1 (MQ=255)

                                                              aCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGtt                          >  1:911260/1‑93 (MQ=255)

                                                              aCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGtt                          >  2:3099784/1‑93 (MQ=255)

                                                               ccAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtc                         >  1:1394036/1‑93 (MQ=255)

                                                               ccAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtc                         <  1:3718990/93‑1 (MQ=255)

                                                               ccAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtc                         <  2:278471/93‑1 (MQ=255)

                                                                cAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGGCTCCGGCGTGTTCGCCCGGATGGCTGTCCTGTACGGATAACTGGATCCTCGTtct                        >  1:2576598/1‑93 (MQ=255)

                                                                 aGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtctc                       >  2:1189784/1‑93 (MQ=255)

                                                                 aGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtctc                       <  2:818324/93‑1 (MQ=255)

                                                                  gcgcCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcc                      >  1:430350/1‑93 (MQ=255)

                                                                     ccgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctct                   >  2:889049/1‑93 (MQ=255)

                                                                      cgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctc                  <  2:453264/93‑1 (MQ=255)

                                                                      cgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctc                  <  1:3707256/93‑1 (MQ=255)

                                                                       gcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctct                 >  1:1670004/1‑93 (MQ=255)

                                                                       gcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctct                 >  1:4045240/1‑93 (MQ=255)

                                                                       gcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctct                 >  1:1271118/1‑93 (MQ=255)

                                                                        cAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCtt                >  2:331850/1‑93 (MQ=255)

                                                                         aGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTg               >  2:2685566/1‑93 (MQ=255)

                                                                         aGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTg               >  1:2321618/1‑93 (MQ=255)

                                                                          gCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGc              >  2:3161465/1‑93 (MQ=255)

                                                                           cGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCt             >  1:4152031/1‑93 (MQ=255)

                                                                           cGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCt             >  1:1139845/1‑93 (MQ=255)

                                                                            gACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTg            >  2:3795080/1‑93 (MQ=255)

                                                                               cTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAt         <  1:1734961/93‑1 (MQ=255)

                                                                                tGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAtt        <  1:758305/93‑1 (MQ=255)

                                                                                tGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAtt        <  1:1115119/93‑1 (MQ=255)

                                                                                cggCGAATCTCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAtt        <  2:3891002/92‑1 (MQ=255)

                                                                                cggCGAATCACCTCTTCTTTGTCCCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAtt        <  2:3961857/92‑1 (MQ=255)

                                                                                 ggCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGCTCTCCTCTCTTGCTGAATTg       >  1:4398372/1‑93 (MQ=255)

                                                                                  gCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGa      >  1:813223/1‑93 (MQ=255)

                                                                                  gCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGa      <  1:3460110/93‑1 (MQ=255)

                                                                                  gCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGa      >  1:813222/1‑93 (MQ=255)

                                                                                   cGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaa     <  2:532362/93‑1 (MQ=255)

                                                                                    gAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa    >  1:3969204/1‑93 (MQ=255)

                                                                                    gAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa    <  1:485160/93‑1 (MQ=255)

                                                                                      aTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACg  >  1:4345535/1‑93 (MQ=255)

AGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACG  >  NC_000913/2261365‑2261543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  NC_000913/2261361‑2261547

GTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCA                                                                                               >  SRR4302544.4547845/1‑94 (MQ=60)

GTTGAGGGTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCA                                                                                               >  SRR4302544.2597843/1‑94 (MQ=60)

   GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCT                                                                                            >  SRR4302544.2158586/1‑94 (MQ=60)

    AGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCCGCGACCTGGCGAATCACCTC                                                                                           >  SRR4302544.4309820/1‑94 (MQ=60)

    AGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTC                                                                                           <  SRR4302544.938230/94‑1 (MQ=60)

     GGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCT                                                                                          >  SRR4302544.1185978/1‑94 (MQ=60)

     GGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCT                                                                                          <  SRR4302544.1275119/94‑1 (MQ=60)

       TTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTC                                                                                        <  SRR4302544.1660828/94‑1 (MQ=60)

         TGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTT                                                                                      >  SRR4302544.2632244/1‑94 (MQ=60)

           CTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTG                                                                                    <  SRR4302544.3204921/94‑1 (MQ=60)

           CTGTTCGCGCGCCAACATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTG                                                                                    >  SRR4302544.2995168/1‑94 (MQ=60)

             GTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTC                                                                                  >  SRR4302544.4322719/1‑94 (MQ=60)

             GTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTC                                                                                  >  SRR4302544.183799/1‑94 (MQ=60)

              TTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCT                                                                                 <  SRR4302544.1871234/94‑1 (MQ=60)

                CGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCC                                                                               <  SRR4302544.2130192/94‑1 (MQ=60)

                  CGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGG                                                                             >  SRR4302544.1916068/1‑94 (MQ=60)

                     GCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCT                                                                          >  SRR4302544.4121694/1‑94 (MQ=60)

                     GCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCT                                                                          >  SRR4302544.2596172/1‑94 (MQ=60)

                       CAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTT                                                                        >  SRR4302544.3056966/1‑94 (MQ=60)

                       CAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTT                                                                        >  SRR4302544.3952639/1‑94 (MQ=60)

                        AGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTT                                                                       <  SRR4302544.1423523/94‑1 (MQ=60)

                        AGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTT                                                                       >  SRR4302544.2998057/1‑94 (MQ=60)

                         GCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTC                                                                      >  SRR4302544.3687527/1‑94 (MQ=60)

                          CATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCG                                                                     >  SRR4302544.1636755/1‑94 (MQ=60)

                              CCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCG                                                                 >  SRR4302544.1167564/1‑94 (MQ=60)

                              CCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCG                                                                 >  SRR4302544.350960/1‑94 (MQ=60)

                               CATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGG                                                                >  SRR4302544.1238215/1‑94 (MQ=60)

                                ATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGA                                                               >  SRR4302544.2144521/1‑94 (MQ=60)

                                 TTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGAT                                                              <  SRR4302544.556990/94‑1 (MQ=60)

                                    ACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGA                                                           >  SRR4302544.3325887/1‑94 (MQ=60)

                                     CGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGAT                                                          <  SRR4302544.4438653/94‑1 (MQ=60)

                                      GTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTACGGCCTTTTCGCCCGGATGGATG                                                         >  SRR4302544.2487178/1‑94 (MQ=60)

                                       TAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGT                                                        >  SRR4302544.2575053/1‑94 (MQ=60)

                                       TAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGT                                                        <  SRR4302544.646620/94‑1 (MQ=60)

                                        AGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTC                                                       >  SRR4302544.2343799/1‑94 (MQ=60)

                                        AGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTC                                                       >  SRR4302544.3928540/1‑94 (MQ=60)

                                          CCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCT                                                     <  SRR4302544.87253/94‑1 (MQ=60)

                                           CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGTTGGATGTCCTG                                                    >  SRR4302544.1358849/1‑94 (MQ=60)

                                           CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    >  SRR4302544.3686793/1‑94 (MQ=60)

                                            TTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGT                                                   <  SRR4302544.1866716/94‑1 (MQ=60)

                                            TTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGT                                                   >  SRR4302544.3516359/1‑94 (MQ=60)

                                              CTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAC                                                 >  SRR4302544.1631029/1‑94 (MQ=60)

                                              CTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAC                                                 >  SRR4302544.612733/1‑94 (MQ=60)

                                               TGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACG                                                <  SRR4302544.4627600/94‑1 (MQ=60)

                                                 CTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGA                                              <  SRR4302544.2136299/94‑1 (MQ=60)

                                                  TACATTACCGACCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAT                                             <  SRR4302544.1990038/94‑1 (MQ=60)

                                                   ACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATA                                            >  SRR4302544.4012826/1‑94 (MQ=60)

                                                   ACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATA                                            >  SRR4302544.2529677/1‑94 (MQ=60)

                                                     ATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAC                                          >  SRR4302544.4313557/1‑94 (MQ=60)

                                                     ATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAC                                          >  SRR4302544.4313558/1‑94 (MQ=60)

                                                      TTACCGGCCTGCACCAGCGCCGCAGCCACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACT                                         <  SRR4302544.1050039/94‑1 (MQ=60)

                                                       TACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTG                                        <  SRR4302544.3804996/94‑1 (MQ=60)

                                                        ACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGG                                       >  SRR4302544.4001916/1‑94 (MQ=60)

                                                         CCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGA                                      <  SRR4302544.3429577/94‑1 (MQ=60)

                                                          CGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAA                                     >  SRR4302544.1054188/1‑94 (MQ=60)

                                                              CTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATA                                 >  SRR4302544.2411839/1‑94 (MQ=60)

                                                                 CACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTT                              <  SRR4302544.1081665/94‑1 (MQ=60)

                                                                 CACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTT                              >  SRR4302544.920271/1‑94 (MQ=60)

                                                                 CACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTT                              <  SRR4302544.1362013/94‑1 (MQ=60)

                                                                  ACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTC                             >  SRR4302544.1407946/1‑94 (MQ=60)

                                                                   CCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCT                            <  SRR4302544.1472222/94‑1 (MQ=60)

                                                                   CCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCT                            <  SRR4302544.3755979/94‑1 (MQ=60)

                                                                   CCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCT                            <  SRR4302544.4564629/94‑1 (MQ=60)

                                                                   CCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGGCTCCGGCGTGTTCGCCCGGATGGCTGTCCTGTACGGATAACTGGAtcctcgttct                            >  SRR4302544.2601350/1‑84 (MQ=60)

                                                                    CAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTC                           >  SRR4302544.857073/1‑94 (MQ=60)

                                                                     AGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCC                          >  SRR4302544.434712/1‑94 (MQ=60)

                                                                       CGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTC                        <  SRR4302544.3026262/94‑1 (MQ=60)

                                                                          CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCT                     >  SRR4302544.4085535/1‑94 (MQ=60)

                                                                          CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCT                     >  SRR4302544.4191968/1‑94 (MQ=60)

                                                                          CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCT                     >  SRR4302544.1283621/1‑94 (MQ=60)

                                                                          CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCT                     <  SRR4302544.3744144/94‑1 (MQ=60)

                                                                          CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCT                     >  SRR4302544.1686460/1‑94 (MQ=60)

                                                                           GCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTT                    >  SRR4302544.3113532/1‑94 (MQ=60)

                                                                            CAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTG                   >  SRR4302544.652324/1‑94 (MQ=60)

                                                                            CAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTG                   >  SRR4302544.2343619/1‑94 (MQ=60)

                                                                              GCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCT                 >  SRR4302544.4193152/1‑94 (MQ=60)

                                                                              GCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCT                 >  SRR4302544.1151180/1‑94 (MQ=60)

                                                                                  CCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAT             >  SRR4302544.816285/1‑94 (MQ=60)

                                                                                  CCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAT             <  SRR4302544.2987987/94‑1 (MQ=60)

                                                                                   CTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATT            <  SRR4302544.1751962/94‑1 (MQ=60)

                                                                                    TGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTG           <  SRR4302544.1126248/94‑1 (MQ=60)

                                                                                    TGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTG           <  SRR4302544.765705/94‑1 (MQ=60)

                                                                                    TGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGCTCTCCTCTCTTGCTGAATTG           >  SRR4302544.4442835/1‑94 (MQ=60)

                                                                                     GGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGA          >  SRR4302544.821160/1‑94 (MQ=60)

                                                                                     GGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGA          >  SRR4302544.821159/1‑94 (MQ=60)

                                                                                      GCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAA         <  SRR4302544.3493134/94‑1 (MQ=60)

                                                                                       CGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAA        >  SRR4302544.4008682/1‑94 (MQ=60)

                                                                                        GAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAC       <  SRR4302544.489910/94‑1 (MQ=60)

                                                                                        GAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAC       <  SRR4302544.1414835/94‑1 (MQ=60)

                                                                                         AATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACG      >  SRR4302544.4389714/1‑94 (MQ=60)

                                                                                           TCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGAT    <  SRR4302544.606980/94‑1 (MQ=60)

                                                                                             CCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  <  SRR4302544.3052574/94‑1 (MQ=60)

                                                                                             ACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  <  SRR4302544.2492365/94‑1 (MQ=60)

                                                                                             ACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  SRR4302544.3511240/1‑94 (MQ=60)

GTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  NC_000913/2261361‑2261547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`