Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,261,454 G→A A21V (GCG→GTG)  fruB ← PTS system, fructose‑specific IIA/fpr component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,261,4540GA99.6% 850.1 / NA 250A21V (GCG→GTG) fruBPTS system, fructose‑specific IIA/fpr component
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (115/132);  total (115/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 261 total aligned reads displayed.

GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACG  >  NC_000913/2261364‑2261543
                                                                                          |                                                                                         
gAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCAcc                                                                                         <  2:1979776/93‑1 (MQ=255)
 aGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACct                                                                                        <  1:510595/93‑1 (MQ=255)
 aGGATTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACct                                                                                        >  1:3924462/1‑93 (MQ=255)
  ggTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACctc                                                                                       >  2:3827305/1‑93 (MQ=255)
   gTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACctct                                                                                      <  1:4858288/93‑1 (MQ=255)
     ttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtcttc                                                                                    <  2:428460/93‑1 (MQ=255)
      tGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtcttct                                                                                   <  2:135180/93‑1 (MQ=255)
      tGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtcttct                                                                                   <  2:2169278/93‑1 (MQ=255)
       gCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTcttctt                                                                                  >  2:613209/1‑93 (MQ=255)
        cTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCttt                                                                                 >  1:6298981/1‑93 (MQ=255)
        cTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCttt                                                                                 <  2:4739401/93‑1 (MQ=255)
         tGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTg                                                                                >  2:3222262/1‑93 (MQ=255)
           ttCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGtc                                                                              <  1:3514975/93‑1 (MQ=255)
           ttCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGtc                                                                              <  1:1350755/93‑1 (MQ=255)
              gcgcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTcc                                                                           >  1:5515451/1‑93 (MQ=255)
                gcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCgg                                                                         >  2:6381501/1‑93 (MQ=255)
                 cgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGc                                                                        >  2:2981256/1‑93 (MQ=255)
                  gcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGcc                                                                       >  2:3308931/1‑93 (MQ=255)
                    cAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCtt                                                                     <  2:6622086/93‑1 (MQ=255)
                     aGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCttt                                                                    <  1:1904505/93‑1 (MQ=255)
                     aGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCttt                                                                    <  2:2308585/93‑1 (MQ=255)
                     aGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCttt                                                                    >  1:2793665/1‑93 (MQ=255)
                      gCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCtttt                                                                   >  2:6173995/1‑93 (MQ=255)
                      acATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCtttt                                                                   <  1:1188403/92‑1 (MQ=255)
                       gaTGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTc                                                                  <  2:2285509/92‑1 (MQ=255)
                        aTGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCg                                                                 >  1:414/1‑93 (MQ=255)
                         tGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGc                                                                >  2:1102465/1‑93 (MQ=255)
                          gCCATTGACGTAGCCTTCTGCTACATTACCCTCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCCGCCTTTTCGcc                                                               >  1:5879357/1‑93 (MQ=255)
                            cATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCg                                                             >  2:6334248/1‑93 (MQ=255)
                            cATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCg                                                             >  1:5819970/1‑93 (MQ=255)
                             aTTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgg                                                            <  2:3661855/93‑1 (MQ=255)
                             aTTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgg                                                            >  2:1165720/1‑93 (MQ=255)
                             aTTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgg                                                            <  1:766309/93‑1 (MQ=255)
                              ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgga                                                           <  1:6171122/93‑1 (MQ=255)
                               tGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggat                                                          <  2:5088476/93‑1 (MQ=255)
                                  cGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatgga                                                       <  1:6416981/93‑1 (MQ=255)
                                  cGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatgga                                                       <  1:2535915/93‑1 (MQ=255)
                                   gTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatggat                                                      <  2:3481073/93‑1 (MQ=255)
                                     aGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatggatat                                                    >  2:4074289/1‑91 (MQ=255)
                                     aGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGt                                                    <  2:420113/93‑1 (MQ=255)
                                      tccTTCTGCTACATTACCGGCCTGCCCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTc                                                   <  1:3632784/92‑1 (MQ=255)
                                      gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTc                                                   >  2:1703106/1‑93 (MQ=255)
                                       ccTTCTGCTGCATTACCGGCCTGCACCATCGCCGCAGCGACCTGGCGAATCCCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTcc                                                  <  1:2453946/93‑1 (MQ=255)
                                       ccTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTcc                                                  <  2:6030180/93‑1 (MQ=255)
                                        cTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCt                                                 <  1:865040/93‑1 (MQ=255)
                                        cTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCt                                                 <  2:2113745/93‑1 (MQ=255)
                                        cTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCt                                                 <  1:3744142/93‑1 (MQ=255)
                                         ttCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTg                                                <  2:4311354/93‑1 (MQ=255)
                                         ttCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTg                                                <  1:1700785/93‑1 (MQ=255)
                                         ttCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTg                                                >  2:4777495/1‑93 (MQ=255)
                                         ttCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTg                                                >  1:3103754/1‑93 (MQ=255)
                                         ttCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTg                                                >  1:6006550/1‑93 (MQ=255)
                                           cTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTa                                              <  2:6681284/93‑1 (MQ=255)
                                               tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGa                                          >  2:3958245/1‑93 (MQ=255)
                                               tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGa                                          >  1:2334716/1‑93 (MQ=255)
                                               tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGa                                          >  2:6013406/1‑93 (MQ=255)
                                                aCATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAt                                         <  2:5420077/93‑1 (MQ=255)
                                                 cATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATa                                        <  2:6299071/93‑1 (MQ=255)
                                                 cATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATa                                        >  1:2031846/1‑93 (MQ=255)
                                                 cATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATa                                        >  2:4893829/1‑93 (MQ=255)
                                                  aTTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATaa                                       >  2:371592/1‑93 (MQ=255)
                                                  aTTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATaa                                       >  2:2884470/1‑93 (MQ=255)
                                                     aCCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTg                                    >  2:441176/1‑93 (MQ=255)
                                                       cGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGa                                  <  2:891019/93‑1 (MQ=255)
                                                        ggCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGaa                                 >  2:1741695/1‑93 (MQ=255)
                                                          ccTGCCCCAGCGCCGCAGCGACCTGGCGAATCCCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACa                               <  1:3693312/93‑1 (MQ=255)
                                                           cagCACCAGCCCTGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCGTTTCGCCCGGATGGATGTCCTGTACCGATAACTGGAACAt                              <  1:4500763/91‑1 (MQ=255)
                                                            tGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATa                             <  1:1098392/93‑1 (MQ=255)
                                                              cACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGt                           <  2:6504658/93‑1 (MQ=255)
                                                               cccAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGtt                          <  2:2599814/92‑1 (MQ=255)
                                                               aCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGtt                          >  2:4981563/1‑93 (MQ=255)
                                                               aCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGtt                          >  1:154039/1‑93 (MQ=255)
                                                               aCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGtt                          >  1:532508/1‑93 (MQ=255)
                                                                 cAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtct                        <  1:1879073/93‑1 (MQ=255)
                                                                  aGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtctc                       <  2:1548844/93‑1 (MQ=255)
                                                                  aGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtctc                       >  1:300125/1‑93 (MQ=255)
                                                                   gcgcCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcc                      <  2:2542742/93‑1 (MQ=255)
                                                                     gccgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcctc                    >  2:2487709/1‑93 (MQ=255)
                                                                       cgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctc                  <  2:680133/93‑1 (MQ=255)
                                                                       cgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctc                  <  2:4582236/93‑1 (MQ=255)
                                                                       cgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctc                  <  1:1580389/93‑1 (MQ=255)
                                                                       cgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctc                  <  1:3324081/93‑1 (MQ=255)
                                                                        gcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctct                 <  2:1957068/93‑1 (MQ=255)
                                                                        gcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctct                 >  1:4604290/1‑93 (MQ=255)
                                                                        gcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctct                 >  1:381219/1‑93 (MQ=255)
                                                                         cAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCtt                >  2:5716903/1‑93 (MQ=255)
                                                                         cAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCtt                <  1:6711070/93‑1 (MQ=255)
                                                                           gCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGc              >  2:2672544/1‑93 (MQ=255)
                                                                           gCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGc              <  1:1409300/93‑1 (MQ=255)
                                                                            cGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCt             >  1:4745764/1‑93 (MQ=255)
                                                                              gccTGGCGAATCACCTCTTCTTTGTCTCCGGCCTGTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGa           <  1:4294976/92‑1 (MQ=255)
                                                                              aCCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGa           >  2:2062128/1‑93 (MQ=255)
                                                                               ccTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGaa          <  2:2909816/93‑1 (MQ=255)
                                                                                cTGACGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAt         >  2:5546893/1‑93 (MQ=255)
                                                                                 tGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAtt        >  1:2926356/1‑93 (MQ=255)
                                                                                  ggCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTGCTCTCTTGCTGAATTg       >  2:2738188/1‑93 (MQ=255)
                                                                                  ggCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTg       <  1:2129471/93‑1 (MQ=255)
                                                                                      aaTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAc   <  1:4046209/93‑1 (MQ=255)
                                                                                      aaTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAc   >  2:6428555/1‑93 (MQ=255)
                                                                                       aTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACg  >  2:18015/1‑93 (MQ=255)
                                                                                          |                                                                                         
GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACG  >  NC_000913/2261364‑2261543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  NC_000913/2261361‑2261547
                                                                                             |                                                                                             
GTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCA                                                                                               >  SRR4302536.516979/1‑94 (MQ=60)
GTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCA                                                                                               >  SRR4302536.704770/1‑94 (MQ=60)
 TTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCAC                                                                                              <  SRR4302536.3393783/94‑1 (MQ=60)
  TGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACC                                                                                             <  SRR4302536.3241110/94‑1 (MQ=60)
   GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGTCGAATCACCT                                                                                            >  SRR4302536.1688252/1‑94 (MQ=60)
   GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCT                                                                                            >  SRR4302536.2881126/1‑94 (MQ=60)
   GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCT                                                                                            >  SRR4302536.473867/1‑94 (MQ=60)
   GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCT                                                                                            <  SRR4302536.1609341/94‑1 (MQ=60)
   GAGGATTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCT                                                                                            >  SRR4302536.4041081/1‑94 (MQ=60)
    AGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTC                                                                                           <  SRR4302536.523693/94‑1 (MQ=60)
     GGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCT                                                                                          >  SRR4302536.1507112/1‑94 (MQ=60)
     GGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCT                                                                                          >  SRR4302536.6638429/1‑94 (MQ=60)
      GTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTT                                                                                         <  SRR4302536.5004497/94‑1 (MQ=60)
       TTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTC                                                                                        <  SRR4302536.6325147/94‑1 (MQ=60)
         TGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTT                                                                                      <  SRR4302536.3853405/94‑1 (MQ=60)
         TGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTT                                                                                      >  SRR4302536.1922123/1‑94 (MQ=60)
          GCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTT                                                                                     <  SRR4302536.6572317/94‑1 (MQ=60)
          GCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTT                                                                                     >  SRR4302536.6486626/1‑94 (MQ=60)
          GCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTT                                                                                     >  SRR4302536.3767837/1‑94 (MQ=60)
          GCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTT                                                                                     <  SRR4302536.3809650/94‑1 (MQ=60)
          GCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTT                                                                                     <  SRR4302536.2166469/94‑1 (MQ=60)
          GCTGTACGCGCGCCCGCATACCATTGACGTACCTTTCTGCTACATTACCGGCCTGCACCAGCACCGCAGCGACGTGGCGAATCATCTCTTCTTT                                                                                     <  SRR4302536.3515151/94‑1 (MQ=53)
           CTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTG                                                                                    >  SRR4302536.6065190/1‑94 (MQ=60)
              TTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCT                                                                                 <  SRR4302536.3619938/94‑1 (MQ=60)
              TTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCT                                                                                 <  SRR4302536.1391031/94‑1 (MQ=60)
                CGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTTGCGAATAACCTCTTCTTTGgcttc                                                                               >  SRR4302536.2662617/1‑89 (MQ=60)
                CGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCC                                                                               >  SRR4302536.4697042/1‑94 (MQ=60)
                CGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCC                                                                               >  SRR4302536.5679939/1‑94 (MQ=60)
                 GCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCG                                                                              <  SRR4302536.1353116/94‑1 (MQ=60)
                  CGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGG                                                                             <  SRR4302536.1522291/94‑1 (MQ=60)
                  CGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGG                                                                             >  SRR4302536.532954/1‑94 (MQ=60)
                   GCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGC                                                                            >  SRR4302536.3682721/1‑94 (MQ=60)
                   GCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGC                                                                            >  SRR4302536.5147403/1‑94 (MQ=60)
                    CGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCC                                                                           <  SRR4302536.691191/94‑1 (MQ=60)
                     GCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCT                                                                          <  SRR4302536.4503658/94‑1 (MQ=60)
                     GCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCACCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCT                                                                          <  SRR4302536.3195105/94‑1 (MQ=60)
                      CCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTT                                                                         <  SRR4302536.1146374/94‑1 (MQ=60)
                      CCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTT                                                                         <  SRR4302536.5603814/94‑1 (MQ=60)
                       CAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTT                                                                        >  SRR4302536.2876567/1‑94 (MQ=60)
                        acatTGCCATTAACGTAGCCTTCTGCTACATTACCGCCCTGCACCAGCCCCCCACCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTT                                                                       >  SRR4302536.5058209/5‑94 (MQ=60)
                        AGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTT                                                                       <  SRR4302536.1961849/94‑1 (MQ=60)
                         ACATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTC                                                                      <  SRR4302536.1224146/94‑1 (MQ=60)
                          CATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCG                                                                     >  SRR4302536.424/1‑94 (MQ=60)
                            TGCCATTGACGTAGCCTTCTGCTACATTACCCTCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCCGCCTTTTCGCC                                                                   >  SRR4302536.6054492/1‑94 (MQ=60)
                             GCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCC                                                                  >  SRR4302536.3185738/1‑94 (MQ=60)
                              CCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCG                                                                 >  SRR4302536.5993352/1‑94 (MQ=60)
                              CCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCG                                                                 >  SRR4302536.853659/1‑94 (MQ=60)
                               CATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGG                                                                >  SRR4302536.573431/1‑94 (MQ=60)
                               CATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGG                                                                >  SRR4302536.4285757/1‑94 (MQ=60)
                                ATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGA                                                               <  SRR4302536.788768/94‑1 (MQ=60)
                                 TTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGAT                                                              <  SRR4302536.6354925/94‑1 (MQ=60)
                                    ACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGA                                                           <  SRR4302536.4915478/94‑1 (MQ=60)
                                     CGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGAT                                                          <  SRR4302536.6608741/94‑1 (MQ=60)
                                     CGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGAT                                                          <  SRR4302536.2612311/94‑1 (MQ=60)
                                      GTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATG                                                         >  SRR4302536.5750009/1‑94 (MQ=60)
                                      GTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATG                                                         >  SRR4302536.6651992/1‑94 (MQ=60)
                                        AGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTC                                                       >  SRR4302536.2083560/1‑94 (MQ=60)
                                         TCCTTCTGCTACATTACCGGCCTGCCCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCC                                                      <  SRR4302536.3741444/94‑1 (MQ=60)
                                         GCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCC                                                      <  SRR4302536.6032164/94‑1 (MQ=60)
                                         GCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCC                                                      <  SRR4302536.3068233/94‑1 (MQ=60)
                                          CCTTCTGCTGCATTACCGGCCTGCACCATCGCCGCAGCGACCTGGCGAATCCCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCT                                                     <  SRR4302536.2528056/94‑1 (MQ=60)
                                           CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    <  SRR4302536.890472/94‑1 (MQ=60)
                                           CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    >  SRR4302536.6185621/1‑94 (MQ=60)
                                           CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    <  SRR4302536.3856072/94‑1 (MQ=60)
                                           CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    >  SRR4302536.3194331/1‑94 (MQ=60)
                                           CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    >  SRR4302536.3720193/1‑94 (MQ=60)
                                            TTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGT                                                   <  SRR4302536.1750004/94‑1 (MQ=60)
                                               TGCAACATTACCGGCCTGCACCAGCGCAGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACG                                                <  SRR4302536.4058097/94‑1 (MQ=60)
                                                 CTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGA                                              >  SRR4302536.2405558/1‑94 (MQ=60)
                                                  TACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAT                                             <  SRR4302536.2448867/94‑1 (MQ=60)
                                                   ACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATA                                            >  SRR4302536.2093036/1‑94 (MQ=60)
                                                     ATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAC                                          <  SRR4302536.4216187/94‑1 (MQ=60)
                                                     ATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAC                                          >  SRR4302536.3590091/1‑94 (MQ=60)
                                                         CCGGCCTGCACCAGCTCCGCAGCGACCTGGCGAATCCCCTCTTCGTTGTCTCCGGCCTTTTCGCCCGGATGTATGTCCTGTACGGATAACTGGA                                      <  SRR4302536.1459710/94‑1 (MQ=60)
                                                         CCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGA                                      >  SRR4302536.4559442/1‑94 (MQ=60)
                                                             CCTGCCCCAGCGCCGCAGCGACCTGGCGAATCCCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACAT                                  <  SRR4302536.3803766/94‑1 (MQ=60)
                                                             CCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAATGGAACAT                                  <  SRR4302536.3440525/94‑1 (MQ=60)
                                                              CAGCACCAGCCCTGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCGTTTCGCCCGGATGGATGTCCTGTACCGATAACTGGAACATA                                 <  SRR4302536.4635832/94‑1 (MQ=60)
                                                               TGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAG                                <  SRR4302536.1131328/94‑1 (MQ=60)
                                                                GCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGT                               <  SRR4302536.1404255/94‑1 (MQ=60)
                                                                 CACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTT                              <  SRR4302536.4297680/94‑1 (MQ=60)
                                                                 CACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTT                              >  SRR4302536.158020/1‑94 (MQ=60)
                                                                 CACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTT                              >  SRR4302536.546115/1‑94 (MQ=60)
                                                                    CAGCGCCGCAGCGACCTGGCGAATGACCTCTTCTTTGTCTCCGGCCTTTTCCCCCGGCTGGCTGTCCTGTACGGAGAACTGGAACATGGTTCTC                           >  SRR4302536.5377234/1‑94 (MQ=60)
                                                                    CAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTC                           <  SRR4302536.1935608/94‑1 (MQ=60)
                                                                    CAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTC                           >  SRR4302536.308093/1‑94 (MQ=60)
                                                                    CAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTC                           >  SRR4302536.3138943/1‑94 (MQ=60)
                                                                     AGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCC                          <  SRR4302536.4824686/94‑1 (MQ=60)
                                                                     AGCGCAGCACCGCCCTGGCGAATCACCTCTTCTTTGTCTCCGTCCTTTTCGCCCGGATGGATGTCCTGTCCGGATAACTGGAACATAGTTCTCC                          <  SRR4302536.2018925/94‑1 (MQ=60)
                                                                      GCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCT                         <  SRR4302536.2876724/94‑1 (MQ=60)
                                                                      GCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCT                         <  SRR4302536.5480670/94‑1 (MQ=60)
                                                                        GCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCT                       >  SRR4302536.1613624/1‑94 (MQ=60)
                                                                        GCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCT                       <  SRR4302536.2182707/94‑1 (MQ=60)
                                                                         CCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTC                      >  SRR4302536.6538579/1‑94 (MQ=60)
                                                                         CCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTA                      <  SRR4302536.1759444/94‑1 (MQ=60)
                                                                          CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCT                     <  SRR4302536.1626681/94‑1 (MQ=60)
                                                                          CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCT                     >  SRR4302536.391263/1‑94 (MQ=60)
                                                                          CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCT                     >  SRR4302536.4742563/1‑94 (MQ=60)
                                                                          CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCT                     <  SRR4302536.3422420/94‑1 (MQ=60)
                                                                           GCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTT                    <  SRR4302536.2455219/94‑1 (MQ=60)
                                                                           GCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTT                    <  SRR4302536.6121217/94‑1 (MQ=60)
                                                                           GCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTT                    <  SRR4302536.2263884/94‑1 (MQ=60)
                                                                            CAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTC                   <  SRR4302536.6910321/94‑1 (MQ=60)
                                                                             AGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGC                  >  SRR4302536.3237325/1‑94 (MQ=60)
                                                                             AGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGC                  <  SRR4302536.3891185/94‑1 (MQ=60)
                                                                              GCGACCTGTCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCT                 <  SRR4302536.2546931/94‑1 (MQ=60)
                                                                              GCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCT                 <  SRR4302536.1451217/94‑1 (MQ=60)
                                                                              GCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCT                 >  SRR4302536.4888586/1‑94 (MQ=60)
                                                                               CGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTG                >  SRR4302536.4119502/1‑94 (MQ=60)
                                                                               CGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTG                <  SRR4302536.6464936/94‑1 (MQ=60)
                                                                                GACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGA               >  SRR4302536.864069/1‑94 (MQ=60)
                                                                                 GCCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTGTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAA              <  SRR4302536.4423334/94‑1 (MQ=60)
                                                                                  CCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAT             >  SRR4302536.1376001/1‑94 (MQ=60)
                                                                                   CTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATT            >  SRR4302536.3012718/1‑94 (MQ=60)
                                                                                    TGGCGAATCACTTCTTCTTTGTCTCCGTCCTTTTCGCCCGGATGGATGTCCTGTACGGATCACTGGAACAAAGTTCCGCTCTCTTGCTGAATTG           <  SRR4302536.3942242/94‑1 (MQ=60)
                                                                                    TGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTG           >  SRR4302536.6311667/1‑94 (MQ=60)
                                                                                     GGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGA          <  SRR4302536.2193413/94‑1 (MQ=60)
                                                                                      GCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAA         <  SRR4302536.3864859/94‑1 (MQ=60)
                                                                                        GAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAC       <  SRR4302536.2846529/94‑1 (MQ=60)
                                                                                         AATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACG      >  SRR4302536.1943988/1‑94 (MQ=60)
                                                                                         AATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACG      <  SRR4302536.4166155/94‑1 (MQ=60)
                                                                                          ATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGA     >  SRR4302536.6229936/1‑94 (MQ=60)
                                                                                          ATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGA     >  SRR4302536.787890/1‑94 (MQ=60)
                                                                                          ATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATATCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGA     >  SRR4302536.5595152/1‑94 (MQ=60)
                                                                                           TCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGAT    >  SRR4302536.4221866/1‑94 (MQ=60)
                                                                                            CACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATT   >  SRR4302536.5471549/1‑94 (MQ=60)
                                                                                            CACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATT   >  SRR4302536.3517682/1‑94 (MQ=60)
                                                                                             ACCTCTTCGTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  SRR4302536.5563049/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  NC_000913/2261361‑2261547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑