Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,296,123 T→C T53A (ACC→GCC)  napC ← cytochrome c‑type protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,296,1230TC98.6% 455.3 / ‑2.6 143T53A (ACC→GCC) napCcytochrome c‑type protein
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (63/78);  total (63/80)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01

Only 100 of 147 total aligned reads displayed.

CGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGTATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGAC  >  NC_000913/2296033‑2296212
                                                                                          |                                                                                         
  ccgcTACGGTTGTTTTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTg                                                                                       <  2:3918512/93‑1 (MQ=255)
  ccgcTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGTATTg                                                                                       <  2:3794442/93‑1 (MQ=255)
  ccgcTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTg                                                                                       <  2:1874766/93‑1 (MQ=255)
  ccgcTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTg                                                                                       <  1:1605876/93‑1 (MQ=255)
  ccgcTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTg                                                                                       <  1:2963082/93‑1 (MQ=255)
  ccgcTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTg                                                                                       <  1:2756857/93‑1 (MQ=255)
  ccgcTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTg                                                                                       <  1:2341456/93‑1 (MQ=255)
   cgcTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTgg                                                                                      <  2:1952042/93‑1 (MQ=255)
    gcTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGc                                                                                     <  2:2807144/93‑1 (MQ=255)
    gcTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGc                                                                                     >  2:2388307/1‑93 (MQ=255)
      tACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCtt                                                                                   <  2:2843695/93‑1 (MQ=255)
       aCGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCttt                                                                                  >  2:1224510/1‑93 (MQ=255)
       aCGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCttt                                                                                  >  1:3677573/1‑93 (MQ=255)
       aCGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCttt                                                                                  >  1:4210409/1‑93 (MQ=255)
        cGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGGTACACCGTGTTGCGCATTTGGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCtttt                                                                                 >  1:2085983/1‑93 (MQ=255)
        cGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCtttt                                                                                 <  1:1084431/93‑1 (MQ=255)
        cGGTTGTTGTAGGGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCtttt                                                                                 <  1:2354737/93‑1 (MQ=255)
         ggTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCttttt                                                                                >  2:601862/1‑93 (MQ=255)
         ggTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCttttt                                                                                <  2:4124623/93‑1 (MQ=255)
          gttgttGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTc                                                                               <  2:798501/93‑1 (MQ=255)
          gttgttGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTc                                                                               <  1:4248532/93‑1 (MQ=255)
             gttgtAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCAt                                                                            <  2:4243584/93‑1 (MQ=255)
             gttgtAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCAt                                                                            >  1:2409762/1‑93 (MQ=255)
             gttgtAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCAt                                                                            <  2:421461/93‑1 (MQ=255)
             gttatAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGACCTCTTCGGCATTGGCTTTTTCCAt                                                                            <  1:104247/93‑1 (MQ=255)
              ttgtAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATc                                                                           <  2:4629556/93‑1 (MQ=255)
               tgtAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATcc                                                                          <  1:4569273/93‑1 (MQ=255)
                gtAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATccc                                                                         >  1:881949/1‑93 (MQ=255)
                 tAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCg                                                                        <  1:3590759/93‑1 (MQ=255)
                 tAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCg                                                                        <  2:563842/93‑1 (MQ=255)
                 tAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCg                                                                        >  1:4040526/1‑93 (MQ=255)
                   gTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGgt                                                                      <  2:3694894/93‑1 (MQ=255)
                   gTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGgt                                                                      <  2:388182/93‑1 (MQ=255)
                       aCGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTa                                                                  <  2:2489143/93‑1 (MQ=255)
                        cGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTaa                                                                 <  2:3759800/93‑1 (MQ=255)
                        cGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTaa                                                                 <  1:498653/93‑1 (MQ=255)
                           aaTCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGc                                                              >  1:4419755/1‑93 (MQ=255)
                               cATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCAcc                                                          >  1:4480574/1‑93 (MQ=255)
                                aTGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCAccc                                                         >  1:641420/1‑93 (MQ=255)
                                 tGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCAcccc                                                        >  1:3544818/1‑93 (MQ=255)
                                  gTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCa                                                       >  1:1435737/1‑93 (MQ=255)
                                  gTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCa                                                       <  2:2141732/93‑1 (MQ=255)
                                    tttCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCaga                                                     <  2:3729633/92‑1 (MQ=255)
                                     ttCCTGATCCACCGTTTTGCGCATTTCGTGTGAGCTAATTCAGAACTCTTCGGCATTGGCTGTTTCCATCACGGTGTTAAAGCCACCCCagaa                                                    <  1:848610/93‑1 (MQ=255)
                                     ttCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCagaa                                                    <  1:3168334/93‑1 (MQ=255)
                                       ccTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCagaaga                                                  <  1:2409097/93‑1 (MQ=255)
                                       ccTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCagaaga                                                  <  1:4456807/93‑1 (MQ=255)
                                        cTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGAc                                                 <  2:2070998/93‑1 (MQ=255)
                                        cTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGAc                                                 >  2:4477947/1‑93 (MQ=255)
                                         tGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACg                                                >  1:2144759/1‑93 (MQ=255)
                                          gATACACCCGGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGa                                               <  1:1600498/93‑1 (MQ=255)
                                           aTACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGAt                                              <  2:4142902/93‑1 (MQ=255)
                                            tACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATg                                             >  1:4243100/1‑93 (MQ=255)
                                            tACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATg                                             <  1:2172411/93‑1 (MQ=255)
                                            tACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAAAACTCTTCGGCATTGGCTTTTTCCATCACTGTGTTTAATCCACCCCAGAATACGATg                                             >  2:1942224/1‑93 (MQ=255)
                                              cacCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATgcc                                           <  2:799058/93‑1 (MQ=255)
                                              cacCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATgcc                                           >  1:2162836/1‑93 (MQ=255)
                                                ccGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATgccgc                                         >  1:4749575/1‑93 (MQ=255)
                                                ccGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATgccgc                                         <  1:3069195/93‑1 (MQ=255)
                                                  gtgtTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCGACGCCAGAAGACGATGCCGCca                                       >  1:4700196/1‑93 (MQ=255)
                                                  gtgtTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCca                                       <  2:1409885/93‑1 (MQ=255)
                                                  gtgtTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCca                                       <  2:3090190/93‑1 (MQ=255)
                                                  gtgtTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTACATCCCGGTGTTAAAGCCAACCCAGAAGACGATGCCGCca                                       >  2:2227741/1‑93 (MQ=255)
                                                   tgtTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCcaa                                      >  2:4592745/1‑93 (MQ=255)
                                                   tattGCGCAGTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCcaa                                      <  2:1814786/91‑1 (MQ=255)
                                                     ttGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCcaaca                                    >  1:1700515/1‑93 (MQ=255)
                                                       gcgcATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACaaa                                  >  1:3908679/1‑93 (MQ=255)
                                                       gcgcATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACaaa                                  >  2:4454882/1‑93 (MQ=255)
                                                       gcgcATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACaaa                                  >  1:9793/1‑93 (MQ=255)
                                                         gcATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAAc                                <  1:1168745/93‑1 (MQ=255)
                                                           aTTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCg                              >  1:3965190/1‑93 (MQ=255)
                                                             ttCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCTCCCCAGAAGACGATGCCGCCAACAAAACCGAt                            <  2:3945412/93‑1 (MQ=255)
                                                              tCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATc                           <  2:3182081/93‑1 (MQ=255)
                                                               cGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCa                          <  1:917618/93‑1 (MQ=255)
                                                                 tGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAAc                        <  1:2685702/93‑1 (MQ=255)
                                                                  ggCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAAca                       <  1:3408485/93‑1 (MQ=255)
                                                                   gCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAAcag                      >  2:121367/1‑93 (MQ=255)
                                                                    cAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAAcagc                     <  2:2696884/93‑1 (MQ=255)
                                                                     aGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAAcagca                    <  1:2237831/93‑1 (MQ=255)
                                                                     aGCTAATGCAGAACTCTACGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAAcagca                    <  1:4222439/93‑1 (MQ=255)
                                                                      gCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAAcagcag                   >  1:3262629/1‑93 (MQ=255)
                                                                        tAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCg                 >  1:1979349/1‑93 (MQ=255)
                                                                         aaTGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGt                >  1:1984809/1‑93 (MQ=255)
                                                                         aaTGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGt                >  1:2862551/1‑93 (MQ=255)
                                                                             cAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTcccc            <  2:4006307/93‑1 (MQ=255)
                                                                               gAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAg          >  1:1686384/1‑93 (MQ=255)
                                                                               gAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAAAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAg          <  2:430732/93‑1 (MQ=255)
                                                                                aaCTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAgc         <  2:1867284/93‑1 (MQ=255)
                                                                                aaCTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAgc         >  1:4320403/1‑93 (MQ=255)
                                                                                 aCTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAgcg        >  1:2993651/1‑93 (MQ=255)
                                                                                 aCTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAgcg        >  1:4011395/1‑93 (MQ=255)
                                                                                   tctTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGcc      <  1:1904434/93‑1 (MQ=255)
                                                                                   tctTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGcc      >  1:1058298/1‑93 (MQ=255)
                                                                                    ctTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCa     <  1:2828290/93‑1 (MQ=255)
                                                                                    ctTCGGCATTGGCTTTTTCCATCACGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCa     <  2:2623710/93‑1 (MQ=255)
                                                                                     ttCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAg    >  2:4574987/1‑93 (MQ=255)
                                                                                     ttCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAg    >  1:3801201/1‑93 (MQ=255)
                                                                                     ttCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGACACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCaa    >  1:1900040/1‑92 (MQ=255)
                                                                                       cGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGAc  >  2:68889/1‑93 (MQ=255)
                                                                                       cGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGAc  <  1:4141998/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGTATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGAC  >  NC_000913/2296033‑2296212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGACGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGTATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCT  >  NC_000913/2296030‑2296216
                                                                                             |                                                                                             
GGACGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGC                                                                                               <  SRR4302544.3341697/94‑1 (MQ=60)
 GACGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCA                                                                                              <  SRR4302544.2075594/94‑1 (MQ=60)
 GACGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCA                                                                                              <  SRR4302544.1388450/94‑1 (MQ=60)
 GACGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCA                                                                                              <  SRR4302544.2685560/94‑1 (MQ=60)
   CGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATT                                                                                            <  SRR4302544.916127/94‑1 (MQ=60)
     CCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGG                                                                                          <  SRR4302544.1621706/94‑1 (MQ=60)
     CCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGG                                                                                          <  SRR4302544.2363637/94‑1 (MQ=60)
     CCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGG                                                                                          <  SRR4302544.2783392/94‑1 (MQ=60)
     CCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGG                                                                                          <  SRR4302544.2991520/94‑1 (MQ=60)
        CTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTT                                                                                       >  SRR4302544.743485/1‑94 (MQ=60)
         TACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTT                                                                                      >  SRR4302544.4252761/1‑94 (MQ=60)
         TACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTT                                                                                      >  SRR4302544.3714254/1‑94 (MQ=60)
          ACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGGTACACCGTGTTGCGCATTTGGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTT                                                                                     >  SRR4302544.2106049/1‑94 (MQ=60)
           CGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTT                                                                                    <  SRR4302544.1095218/94‑1 (MQ=60)
           CGGTTGTTGTAGGGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTT                                                                                    <  SRR4302544.2377046/94‑1 (MQ=60)
             GTTGTTGTAGTGCACGGAATCCATGTATTGCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGTATTGGCTTTTTCC                                                                                  <  SRR4302544.3279923/94‑1 (MQ=60)
             GTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCC                                                                                  <  SRR4302544.4291851/94‑1 (MQ=60)
               TGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCAT                                                                                >  SRR4302544.2432662/1‑94 (MQ=60)
                GTTATAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGACCTCTTCGGCATTGGCTTTTTCCATC                                                                               <  SRR4302544.105204/94‑1 (MQ=60)
                  TGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCC                                                                             <  SRR4302544.4614820/94‑1 (MQ=60)
                  TGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCC                                                                             >  SRR4302544.890689/1‑94 (MQ=60)
                   GTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCG                                                                            >  SRR4302544.4080763/1‑94 (MQ=60)
                    TAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGG                                                                           <  SRR4302544.3625239/94‑1 (MQ=60)
                     AGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGT                                                                          <  SRR4302544.2939282/94‑1 (MQ=60)
                       TGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGT                                                                        >  SRR4302544.3720103/1‑94 (MQ=60)
                        GCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTT                                                                       <  SRR4302544.1959732/94‑1 (MQ=60)
                           CGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAA                                                                    <  SRR4302544.4486272/94‑1 (MQ=60)
                           CGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAA                                                                    <  SRR4302544.4281271/94‑1 (MQ=60)
                           CGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAA                                                                    <  SRR4302544.503551/94‑1 (MQ=60)
                            GGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAG                                                                   <  SRR4302544.4234749/94‑1 (MQ=60)
                             GAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGC                                                                  >  SRR4302544.4464357/1‑94 (MQ=60)
                              AATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCC                                                                 <  SRR4302544.2172577/94‑1 (MQ=60)
                                 CCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACC                                                              >  SRR4302544.4525612/1‑94 (MQ=60)
                                  CATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCC                                                             >  SRR4302544.647750/1‑94 (MQ=60)
                                   ATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCC                                                            >  SRR4302544.3069640/1‑94 (MQ=60)
                                   ATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCC                                                            >  SRR4302544.3578758/1‑94 (MQ=60)
                                    TGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCA                                                           >  SRR4302544.1450029/1‑94 (MQ=60)
                                     GTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAG                                                          <  SRR4302544.4307629/94‑1 (MQ=60)
                                        TTCCTGATCCACCGTTTTGCGCATTTCGTGTGAGCTAATTCAGAACTCTTCGGCATTGGCTGTTTCCATCACGGTGTTAAAGCCACCCCAGAAG                                                       <  SRR4302544.857001/94‑1 (MQ=60)
                                        TTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAG                                                       <  SRR4302544.3198486/94‑1 (MQ=60)
                                          CCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGAC                                                     >  SRR4302544.4078875/1‑94 (MQ=60)
                                          CCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGAC                                                     <  SRR4302544.4501685/94‑1 (MQ=60)
                                          CCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGAC                                                     <  SRR4302544.2431985/94‑1 (MQ=60)
                                           CTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACG                                                    >  SRR4302544.2165208/1‑94 (MQ=60)
                                            TGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGA                                                   >  SRR4302544.1892773/1‑94 (MQ=60)
                                             GATACACCCGGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGAT                                                  <  SRR4302544.1616284/94‑1 (MQ=60)
                                              ATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATG                                                 >  SRR4302544.4286357/1‑94 (MQ=60)
                                               TACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGC                                                <  SRR4302544.2193004/94‑1 (MQ=60)
                                                ACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCC                                               >  SRR4302544.2183368/1‑94 (MQ=60)
                                                  ACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGC                                             >  SRR4302544.4796354/1‑94 (MQ=60)
                                                   CCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCC                                            <  SRR4302544.3098444/94‑1 (MQ=60)
                                                    CGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCGACGCCAGAAGACGATGCCGCCA                                           >  SRR4302544.4746633/1‑94 (MQ=60)
                                                     GTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAA                                          >  SRR4302544.4003292/1‑94 (MQ=60)
                                                       GTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACA                                        >  SRR4302544.1717296/1‑94 (MQ=60)
                                                         TGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAA                                      >  SRR4302544.3947516/1‑94 (MQ=60)
                                                         TGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAA                                      >  SRR4302544.9882/1‑94 (MQ=60)
                                                            GCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACC                                   <  SRR4302544.2420653/94‑1 (MQ=60)
                                                            GCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACC                                   <  SRR4302544.1180400/94‑1 (MQ=60)
                                                             CATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCG                                  <  SRR4302544.3537104/94‑1 (MQ=60)
                                                             CATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCG                                  >  SRR4302544.4004639/1‑94 (MQ=60)
                                                             CATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCG                                  >  SRR4302544.454313/1‑94 (MQ=60)
                                                              ATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCCGAAGACGATGCCGCCAACAAAACCGT                                 >  SRR4302544.1165061/1‑94 (MQ=60)
                                                                  CGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAA                             <  SRR4302544.926678/94‑1 (MQ=60)
                                                                    TGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACA                           <  SRR4302544.2711439/94‑1 (MQ=60)
                                                                    TGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACA                           >  SRR4302544.1650575/1‑94 (MQ=60)
                                                                     GGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAG                          <  SRR4302544.3441075/94‑1 (MQ=60)
                                                                       CAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGGCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCA                        <  SRR4302544.2007469/94‑1 (MQ=60)
                                                                        CGCTAATGTAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAG                       <  SRR4302544.2258888/94‑1 (MQ=60)
                                                                        AGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAG                       <  SRR4302544.492684/94‑1 (MQ=60)
                                                                        AGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAG                       <  SRR4302544.2258887/94‑1 (MQ=60)
                                                                        AGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAG                       >  SRR4302544.3293788/1‑94 (MQ=60)
                                                                        AGCTAATGCAGAACTCTACGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAG                       <  SRR4302544.4265283/94‑1 (MQ=60)
                                                                          CTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCG                     >  SRR4302544.1998577/1‑94 (MQ=60)
                                                                           TAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGT                    >  SRR4302544.2004078/1‑94 (MQ=60)
                                                                           TAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGT                    >  SRR4302544.2890178/1‑94 (MQ=60)
                                                                            AATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTC                   >  SRR4302544.2460034/1‑94 (MQ=60)
                                                                             ATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCC                  <  SRR4302544.279195/94‑1 (MQ=60)
                                                                                 AGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAG              >  SRR4302544.1703008/1‑94 (MQ=60)
                                                                                  GAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGC             >  SRR4302544.4364411/1‑94 (MQ=60)
                                                                                   AACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCG            >  SRR4302544.4051297/1‑94 (MQ=60)
                                                                                   AACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCG            >  SRR4302544.3022354/1‑94 (MQ=60)
                                                                                     CTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCC          >  SRR4302544.1068805/1‑94 (MQ=60)
                                                                                      TCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCA         <  SRR4302544.1922843/94‑1 (MQ=60)
                                                                                      TCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCA         <  SRR4302544.1572660/94‑1 (MQ=60)
                                                                                       CTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAG        <  SRR4302544.2855614/94‑1 (MQ=60)
                                                                                       CTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAG        >  SRR4302544.3838855/1‑94 (MQ=60)
                                                                                       CTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGACACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAA        >  SRR4302544.1918399/1‑94 (MQ=60)
                                                                                          CGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACG     <  SRR4302544.4183002/94‑1 (MQ=60)
                                                                                           GGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGG    >  SRR4302544.1743435/1‑94 (MQ=60)
                                                                                             CATTGGCTTTTTCGATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCG  >  SRR4302544.3633628/1‑94 (MQ=60)
                                                                                             CATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCT  >  SRR4302544.569056/1‑94 (MQ=60)
                                                                                             |                                                                                             
GGACGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGTATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCT  >  NC_000913/2296030‑2296216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑