Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,297,335 A→G G88G (GGT→GGC)  napH ← ferredoxin‑type protein: electron transfer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,297,3350AG99.2% 405.2 / NA 128G88G (GGT→GGC) napHferredoxin‑type protein: electron transfer
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (68/59);  minor base C (0/1);  total (68/60)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

CGCTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCACCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATA  >  NC_000913/2297245‑2297423
                                                                                          |                                                                                        
cGCTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATcaccgcgcc                                                                                        <  1:2130785/93‑1 (MQ=255)
  cTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATAGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCgg                                                                                      <  1:1174959/93‑1 (MQ=255)
   tAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGt                                                                                     <  1:1367303/93‑1 (MQ=255)
   tAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGt                                                                                     <  1:4316624/93‑1 (MQ=255)
     aGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCa                                                                                   >  2:1792325/1‑93 (MQ=255)
      gTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCaa                                                                                  >  2:3720394/1‑93 (MQ=255)
       tCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCTCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAAc                                                                                 <  1:202719/93‑1 (MQ=255)
       tCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAAc                                                                                 >  1:2294010/1‑93 (MQ=255)
       tCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAAc                                                                                 <  2:4556610/93‑1 (MQ=255)
       tCGATAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAAc                                                                                 <  2:200858/93‑1 (MQ=255)
         ggTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGc                                                                               >  1:3073808/1‑93 (MQ=255)
         ggTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGc                                                                               >  2:3453132/1‑93 (MQ=255)
          gTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGcc                                                                              >  2:2749370/1‑93 (MQ=255)
            aaTCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCAc                                                                            <  1:3835272/93‑1 (MQ=255)
            aaTCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCACTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCAc                                                                            >  1:4475850/1‑93 (MQ=255)
             aTCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCAcc                                                                           >  1:3787154/1‑93 (MQ=255)
             aTCGGGTGCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCAcc                                                                           <  1:4468546/93‑1 (MQ=255)
              tCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGTTCAACGCCACCg                                                                          >  2:3311908/1‑93 (MQ=255)
              tCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCg                                                                          >  1:2711353/1‑93 (MQ=255)
               cGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGt                                                                         >  2:250112/1‑93 (MQ=255)
                gggTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTg                                                                        >  1:3155069/1‑93 (MQ=255)
                     cAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTggcacg                                                                   >  2:2384810/1‑91 (MQ=255)
                      aGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTggcaggc                                                                  >  2:3129807/1‑93 (MQ=255)
                      aGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTggcaggc                                                                  >  1:2266684/1‑93 (MQ=255)
                      aGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTggcaggc                                                                  <  2:3824249/93‑1 (MQ=255)
                       gCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTggcaggca                                                                 <  1:4557310/93‑1 (MQ=255)
                        cGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGgcaggcag                                                                <  2:4487808/93‑1 (MQ=255)
                         gggCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGa                                                               <  1:169889/93‑1 (MQ=255)
                         gggCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGa                                                               <  1:3939210/93‑1 (MQ=255)
                         ggacAGAGCCAGCTGCAAAATACTCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGa                                                               <  2:596890/90‑1 (MQ=255)
                          ggCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGAt                                                              >  1:1332949/1‑93 (MQ=255)
                           gCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATg                                                             >  2:3728261/1‑93 (MQ=255)
                           gCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGACACCGTGGCAGGCAGAta                                                             >  1:287178/1‑92 (MQ=255)
                            cAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGa                                                            <  2:189878/93‑1 (MQ=255)
                            cAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGa                                                            <  2:1785190/93‑1 (MQ=255)
                            cAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGa                                                            <  2:1506509/93‑1 (MQ=255)
                              gACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGAcc                                                          >  1:2160076/1‑93 (MQ=255)
                              gACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGAcc                                                          >  1:1661626/1‑93 (MQ=255)
                              gACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGAcc                                                          >  1:27889/1‑93 (MQ=255)
                               aCCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCa                                                         >  1:3380220/1‑93 (MQ=255)
                                cccAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCAc                                                        >  2:755911/1‑93 (MQ=255)
                                  cAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTg                                                      >  2:152231/1‑93 (MQ=255)
                                   aGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTgg                                                     <  1:3332401/93‑1 (MQ=255)
                                         aaaaTAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAATAc                                               >  2:3606999/1‑93 (MQ=255)
                                         aaaaTAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGAc                                               <  1:2673838/93‑1 (MQ=255)
                                           aataatCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACtt                                             <  2:3895458/93‑1 (MQ=255)
                                           aataatCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACtt                                             <  2:3895575/93‑1 (MQ=255)
                                            ataatCTCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCCCCGCCCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACttt                                            <  2:3869553/93‑1 (MQ=255)
                                            ataatCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACttt                                            >  1:193378/1‑93 (MQ=255)
                                               atCCCTTTCCCGCCAGGGCGTAGCGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCa                                         <  1:2139629/93‑1 (MQ=255)
                                                 cGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGc                                       <  1:996641/93‑1 (MQ=255)
                                                  gCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCg                                      >  2:2376743/1‑93 (MQ=255)
                                                   gtttCTCGCCAGGGCGTAGAGCACAGTGATAATCACCGCGCCGGTCAACGCCGCCGTGGCAGGCAGATGAACACTGGCAAGACTTTGCAGCGt                                     <  2:3241773/92‑1 (MQ=255)
                                                    tttCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGtc                                    >  2:4116312/1‑93 (MQ=255)
                                                     ttCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGtca                                   >  2:3056206/1‑93 (MQ=255)
                                                       cccGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGtcatc                                 <  1:732246/93‑1 (MQ=255)
                                                       cccGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGtcatc                                 <  2:1252056/93‑1 (MQ=255)
                                                        ccGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGtcatca                                <  2:3583027/93‑1 (MQ=255)
                                                        ccGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGtcatca                                >  1:4125114/1‑93 (MQ=255)
                                                         cGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAg                               >  1:883711/1‑93 (MQ=255)
                                                         cGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAg                               <  2:1906234/93‑1 (MQ=255)
                                                           ccAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCg                             >  1:4685247/1‑93 (MQ=255)
                                                           ccAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCg                             >  1:2949396/1‑93 (MQ=255)
                                                            cAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCgg                            >  2:527292/1‑93 (MQ=255)
                                                             aGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGa                           >  1:1026568/1‑93 (MQ=255)
                                                              gggCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGAt                          >  1:3949624/1‑93 (MQ=255)
                                                              gggCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGAt                          <  2:4599816/93‑1 (MQ=255)
                                                              gggCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGAt                          <  2:3203953/93‑1 (MQ=255)
                                                                  gTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGt                      <  1:104916/93‑1 (MQ=255)
                                                                  gTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGt                      >  2:2985550/1‑93 (MQ=255)
                                                                  gTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGt                      <  1:104915/93‑1 (MQ=255)
                                                                   tAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTc                     >  1:1648560/1‑93 (MQ=255)
                                                                   tAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTc                     <  1:4393275/93‑1 (MQ=255)
                                                                    agagCACGGTGATAATCACCGTGCCGGTCAACGCCACCGTGGCAGGCAGATTATCATTGGCAAGACCTTGCAGCGTCATCAGCTGATCGGTCa                    >  2:4217143/1‑93 (MQ=255)
                                                                     gagCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAg                   >  1:3124026/1‑93 (MQ=255)
                                                                      agCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGc                  <  2:951885/93‑1 (MQ=255)
                                                                      agCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGc                  <  2:951886/93‑1 (MQ=255)
                                                                      agCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGc                  >  1:1746349/1‑93 (MQ=255)
                                                                      agCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGc                  >  1:861531/1‑93 (MQ=255)
                                                                          cGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGaa              <  2:589420/93‑1 (MQ=255)
                                                                          cGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGaa              >  1:752099/1‑93 (MQ=255)
                                                                           ggTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAAc             <  1:4072735/93‑1 (MQ=255)
                                                                             tGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACgg           <  2:2613316/93‑1 (MQ=255)
                                                                             tGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACgg           >  1:1544836/1‑93 (MQ=255)
                                                                             tGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACgg           <  2:143059/93‑1 (MQ=255)
                                                                             tGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACgg           <  2:2613315/93‑1 (MQ=255)
                                                                              gATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACggg          >  2:3631620/1‑92 (MQ=255)
                                                                              gATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACggg          >  2:2784447/1‑92 (MQ=255)
                                                                              gATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACggg          >  1:1104245/1‑92 (MQ=255)
                                                                              gATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGgt          <  1:4739876/93‑1 (MQ=255)
                                                                                tAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGTCAAGACTTTGCAGCTTCATCAGCGGAGCGGTCAGCGGAACGgtgt        <  1:735102/93‑1 (MQ=255)
                                                                                tAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGgtgt        <  1:936266/93‑1 (MQ=255)
                                                                                tAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGgtgt        >  2:1959774/1‑93 (MQ=255)
                                                                                tAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGgtgt        >  2:786265/1‑93 (MQ=255)
                                                                                 aaTCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGgggtg       >  1:1688442/1‑92 (MQ=255)
                                                                                 aaTCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTc       <  1:4074522/93‑1 (MQ=255)
                                                                                     cccgcgccgGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAAt   <  2:2095417/92‑1 (MQ=255)
                                                                                     accgcgccgGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAAt   >  1:3753827/1‑93 (MQ=255)
                                                                                      ccgcgccgGTCAACGCCACCGTGGCAGGCAGCTGACCACTGGCAGGACTTTGCAGCGTCATCAGCGGATCAGTCAGCGGAACGTTGTCGAATa  <  2:1165685/93‑1 (MQ=255)
                                                                                      ccgcgccgGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATa  <  2:1481092/93‑1 (MQ=255)
                                                                                          |                                                                                        
CGCTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCACCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATA  >  NC_000913/2297245‑2297423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCACCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAACAG  >  NC_000913/2297245‑2297427
                                                                                          |                                                                                            
CGCTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCG                                                                                           <  SRR4302544.2151189/94‑1 (MQ=60)
  CTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATAGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGT                                                                                         <  SRR4302544.1186675/94‑1 (MQ=60)
   TAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTC                                                                                        <  SRR4302544.4360594/94‑1 (MQ=60)
   TAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTC                                                                                        <  SRR4302544.1380896/94‑1 (MQ=60)
      GTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAAC                                                                                     >  SRR4302544.2315634/1‑94 (MQ=60)
       TCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCTCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACG                                                                                    <  SRR4302544.204780/94‑1 (MQ=60)
        CGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGC                                                                                   >  SRR4302544.3103105/1‑94 (MQ=60)
         GGAAATCGGATTCCGAGGGCAGTCCCAGATGCAGAATAATCACATACCCGCCAGGGCGTAGAGCACGGTGATACTCACCGCGCCGGTAAACGCC                                                                                  <  SRR4302544.4245437/94‑1 (MQ=21)
           TAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCACTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCAC                                                                                >  SRR4302544.4520858/1‑94 (MQ=60)
            AATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACC                                                                               <  SRR4302544.3873300/94‑1 (MQ=60)
            AATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACC                                                                               >  SRR4302544.3824655/1‑94 (MQ=60)
             ATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCG                                                                              >  SRR4302544.2737429/1‑94 (MQ=60)
             ATCGGGTGCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCG                                                                              <  SRR4302544.4513493/94‑1 (MQ=60)
               CGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTG                                                                            >  SRR4302544.3185082/1‑94 (MQ=60)
                     CAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGC                                                                      >  SRR4302544.2288011/1‑94 (MQ=60)
                       GCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAG                                                                    <  SRR4302544.4602780/94‑1 (MQ=60)
                         GGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGAT                                                                  <  SRR4302544.171561/94‑1 (MQ=60)
                         GGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGAT                                                                  <  SRR4302544.3978336/94‑1 (MQ=60)
                         GGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGAT                                                                  >  SRR4302544.1346076/1‑94 (MQ=60)
                         GGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCACGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCcgccagag                                                                  >  SRR4302544.1346075/1‑86 (MQ=60)
                          GGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGACACCGTGGCAGGCAGATA                                                                 >  SRR4302544.290161/1‑94 (MQ=60)
                             AGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACC                                                              >  SRR4302544.2180595/1‑94 (MQ=60)
                             AGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACC                                                              >  SRR4302544.28156/1‑94 (MQ=60)
                             AGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACC                                                              >  SRR4302544.1677989/1‑94 (MQ=60)
                             AGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACC                                                              >  SRR4302544.1910421/1‑94 (MQ=60)
                             AGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACC                                                              >  SRR4302544.1677990/1‑94 (MQ=60)
                              GACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCA                                                             >  SRR4302544.3412493/1‑94 (MQ=60)
                                  CAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGG                                                         >  SRR4302544.1641075/1‑94 (MQ=60)
                                   AGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGC                                                        <  SRR4302544.791170/94‑1 (MQ=60)
                                   AGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGC                                                        <  SRR4302544.3364135/94‑1 (MQ=60)
                                       GCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAACA                                                    >  SRR4302544.2816932/1‑94 (MQ=60)
                                         AAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACT                                                  <  SRR4302544.2699469/94‑1 (MQ=60)
                                           AATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTT                                                >  SRR4302544.195324/1‑94 (MQ=60)
                                              AATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCA                                             >  SRR4302544.2469665/1‑94 (MQ=60)
                                               ATCTCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAG                                            <  SRR4302544.2077585/94‑1 (MQ=60)
                                               ATCCCTTTCCCGCCAGGGCGTAGCGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAG                                            <  SRR4302544.2160057/94‑1 (MQ=60)
                                                 ccctcTCCCGCCAGGGCGTAGAGCACGGGTATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACGGGCAAGACTTTGCAGCG                                          <  SRR4302544.1006500/89‑1 (MQ=60)
                                                 CGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCG                                          <  SRR4302544.1006501/94‑1 (MQ=60)
                                                      TCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATC                                     <  SRR4302544.497336/94‑1 (MQ=60)
                                                       CCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCA                                    >  SRR4302544.4166038/1‑94 (MQ=60)
                                                       CCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCA                                    <  SRR4302544.739408/94‑1 (MQ=60)
                                                        CCGCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAG                                   >  SRR4302544.892468/1‑94 (MQ=60)
                                                          GCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCG                                 >  SRR4302544.4731559/1‑94 (MQ=60)
                                                          GCCAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCG                                 >  SRR4302544.2977722/1‑94 (MQ=60)
                                                            CAGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGA                               >  SRR4302544.1036810/1‑94 (MQ=60)
                                                             AGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGAT                              >  SRR4302544.3988875/1‑94 (MQ=60)
                                                             AGGGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGAT                              <  SRR4302544.2182200/94‑1 (MQ=60)
                                                               GGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCG                            <  SRR4302544.3503384/94‑1 (MQ=60)
                                                               GGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCG                            <  SRR4302544.3959893/94‑1 (MQ=60)
                                                               GGCGTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCG                            <  SRR4302544.4461016/94‑1 (MQ=60)
                                                                  GTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTC                         >  SRR4302544.1664825/1‑94 (MQ=60)
                                                                  GTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTC                         <  SRR4302544.105879/94‑1 (MQ=60)
                                                                  GTAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTC                         <  SRR4302544.105880/94‑1 (MQ=60)
                                                                   TAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCA                        <  SRR4302544.333428/94‑1 (MQ=60)
                                                                   TAGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCA                        <  SRR4302544.4437710/94‑1 (MQ=60)
                                                                    AGAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAG                       >  SRR4302544.3153721/1‑94 (MQ=60)
                                                                     GAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGC                      >  SRR4302544.870054/1‑94 (MQ=60)
                                                                     GAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGC                      <  SRR4302544.4715720/94‑1 (MQ=60)
                                                                     GAGCACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGC                      >  SRR4302544.1763447/1‑94 (MQ=60)
                                                                         ACGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAA                  >  SRR4302544.759441/1‑94 (MQ=60)
                                                                          CGGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAAC                 <  SRR4302544.4092881/94‑1 (MQ=60)
                                                                           GGTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACG                <  SRR4302544.4113264/94‑1 (MQ=60)
                                                                            GTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGG               >  SRR4302544.111229/1‑94 (MQ=60)
                                                                            GTGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGG               >  SRR4302544.1560221/1‑94 (MQ=60)
                                                                             TGATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGG              >  SRR4302544.1115241/1‑94 (MQ=60)
                                                                              GATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTG             <  SRR4302544.4786592/94‑1 (MQ=60)
                                                                              GATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTG             <  SRR4302544.3037904/94‑1 (MQ=60)
                                                                               ATAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGT            <  SRR4302544.895312/94‑1 (MQ=60)
                                                                                TAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGTCAAGACTTTGCAGCTTCATCAGCGGAGCGGTCAGCGGAACGGTGTC           <  SRR4302544.742290/94‑1 (MQ=60)
                                                                                TAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGggtg           >  SRR4302544.1705096/1‑90 (MQ=60)
                                                                                TAATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTC           <  SRR4302544.945493/94‑1 (MQ=60)
                                                                                 AATCACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCG          <  SRR4302544.4115071/94‑1 (MQ=60)
                                                                                    CACCGCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAAT       >  SRR4302544.3791101/1‑94 (MQ=60)
                                                                                        GCGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAACA   <  SRR4302544.1830073/94‑1 (MQ=60)
                                                                                         CGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGTATCGGTCAGCGGAACGGTGTCGAATAACAG  <  SRR4302544.1760638/94‑1 (MQ=60)
                                                                                         CGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAACAG  >  SRR4302544.3991999/1‑94 (MQ=60)
                                                                                         CGCCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAACAG  >  SRR4302544.3088638/1‑94 (MQ=60)
                                                                                          |                                                                                            
CGCTAAGTCGGTAATCGGGTTCAGCGGGCAGACCCAGCTGCAAAATAATCGCTTTCCCGCCAGGGCGTAGAGCACGGTGATAATCACCGCACCGGTCAACGCCACCGTGGCAGGCAGATGACCACTGGCAAGACTTTGCAGCGTCATCAGCGGATCGGTCAGCGGAACGGTGTCGAATAACAG  >  NC_000913/2297245‑2297427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑