Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,296,123 T→C T53A (ACC→GCC)  napC ← cytochrome c‑type protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,296,1230TC99.5% 704.7 / NA 216T53A (ACC→GCC) napCcytochrome c‑type protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (118/97);  total (118/98)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

CGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGTATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGAC  >  NC_000913/2296033‑2296212
                                                                                          |                                                                                         
 gccgcTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCAtt                                                                                        >  1:4684194/1‑93 (MQ=255)
  ccgcTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTg                                                                                       <  2:2125271/93‑1 (MQ=255)
  ccgcTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTg                                                                                       <  1:264459/93‑1 (MQ=255)
    gcTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGc                                                                                     <  2:153538/93‑1 (MQ=255)
    gcTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGc                                                                                     >  1:3059008/1‑93 (MQ=255)
      tACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCtt                                                                                   <  2:1325374/93‑1 (MQ=255)
      tACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCtt                                                                                   <  1:3440021/93‑1 (MQ=255)
      tACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCtt                                                                                   <  1:2008100/93‑1 (MQ=255)
       aCGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCttt                                                                                  >  1:1031172/1‑93 (MQ=255)
       aCGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCttt                                                                                  <  2:2368350/93‑1 (MQ=255)
       aCGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCttt                                                                                  <  2:5979295/93‑1 (MQ=255)
        cGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCtttt                                                                                 <  1:2084329/93‑1 (MQ=255)
        cGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCtttt                                                                                 <  2:372846/93‑1 (MQ=255)
        cGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCtttt                                                                                 <  2:1977421/93‑1 (MQ=255)
          gttgttGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTc                                                                               >  1:4950584/1‑93 (MQ=255)
           ttgttgTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTcc                                                                              >  2:6202698/1‑93 (MQ=255)
            tgttgtAGTGCACTGAATCCATGTATTCGTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCa                                                                             <  1:2675401/93‑1 (MQ=255)
            tgttgtAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCa                                                                             <  2:4168056/93‑1 (MQ=255)
             gttgtAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCAt                                                                            >  1:6040421/1‑93 (MQ=255)
              ttgtAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATc                                                                           >  1:2752725/1‑93 (MQ=255)
              ttgtAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATc                                                                           <  2:1797735/93‑1 (MQ=255)
              ttgtAGTGCACGGAATCCATGAATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATc                                                                           >  1:5247790/1‑93 (MQ=255)
               tgtAGTGCACGGAATCCATGTATTCCTGATACCCCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATcc                                                                          <  1:4854707/93‑1 (MQ=255)
                gtAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATccc                                                                         <  1:4140826/93‑1 (MQ=255)
                gtAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATccc                                                                         <  2:4798038/93‑1 (MQ=255)
                  aGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCgg                                                                       >  1:1914791/1‑93 (MQ=255)
                    tGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTGCATCCCGgtg                                                                     >  1:449636/1‑93 (MQ=255)
                    tGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGgtg                                                                     >  1:5653961/1‑93 (MQ=255)
                       aCGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTa                                                                  >  1:5812940/1‑93 (MQ=255)
                        cGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTaa                                                                 <  1:978654/93‑1 (MQ=255)
                         ggAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTaaa                                                                >  1:2050526/1‑93 (MQ=255)
                         ggAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTaaa                                                                >  2:6531721/1‑93 (MQ=255)
                           aaTCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGc                                                              >  1:548903/1‑93 (MQ=255)
                            aTCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGcc                                                             >  1:4512103/1‑93 (MQ=255)
                            aTCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGcc                                                             <  1:5565030/93‑1 (MQ=255)
                            aTCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGcc                                                             >  1:4006042/1‑93 (MQ=255)
                            aTCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGcc                                                             >  2:2830393/1‑93 (MQ=255)
                             tCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCa                                                            <  2:684669/93‑1 (MQ=255)
                              ccATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCAc                                                           >  1:833567/1‑93 (MQ=255)
                               cATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCAcc                                                          >  2:2994034/1‑93 (MQ=255)
                               cATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCAcc                                                          >  2:5527267/1‑93 (MQ=255)
                                aTGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCTGGGGTTAAacccaccc                                                         >  1:1105542/1‑93 (MQ=255)
                                aTGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCAccc                                                         >  1:746897/1‑93 (MQ=255)
                                 tGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCAccac                                                        >  1:2446831/1‑91 (MQ=255)
                                  gTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCa                                                       >  2:5665721/1‑93 (MQ=255)
                                  gTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCa                                                       >  2:5790042/1‑93 (MQ=255)
                                   tATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCag                                                      >  2:4114928/1‑93 (MQ=255)
                                   tATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCag                                                      >  2:3229711/1‑93 (MQ=255)
                                     ttCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCagaa                                                    >  1:1219230/1‑93 (MQ=255)
                                     ttCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCagaa                                                    <  2:2014346/93‑1 (MQ=255)
                                      tCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCagaag                                                   <  2:4655561/93‑1 (MQ=255)
                                       ccTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCagaaga                                                  <  2:4373990/93‑1 (MQ=255)
                                       ccTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCagaaga                                                  <  2:4225954/93‑1 (MQ=255)
                                        cTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGAc                                                 >  1:5428755/1‑93 (MQ=255)
                                        cTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGAc                                                 <  1:3767811/93‑1 (MQ=255)
                                          gATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGa                                               <  2:1213112/93‑1 (MQ=255)
                                          gATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGa                                               >  1:1356183/1‑93 (MQ=255)
                                           aTACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGAt                                              >  2:1617803/1‑93 (MQ=255)
                                           aTACACCGTGTTGCGCATTACGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGAt                                              >  1:6558009/1‑93 (MQ=255)
                                            tACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATg                                             >  1:6049448/1‑93 (MQ=255)
                                            tACACCGGGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATg                                             <  2:5213605/93‑1 (MQ=255)
                                             acacCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATgc                                            >  2:4288591/1‑93 (MQ=255)
                                              cacCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATgcc                                           <  1:2499730/93‑1 (MQ=255)
                                              cacCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATgcc                                           <  1:877026/93‑1 (MQ=255)
                                               acCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATgccg                                          >  1:1229041/1‑93 (MQ=255)
                                                   tgtTGCGCATTTCGTGGCAGCTAATGCAGACCTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCcaa                                      <  1:2846943/93‑1 (MQ=255)
                                                    gtTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCcaac                                     >  2:4371340/1‑93 (MQ=255)
                                                    gtTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCcaac                                     >  2:2771476/1‑93 (MQ=255)
                                                    gtTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCcaac                                     >  2:3189379/1‑93 (MQ=255)
                                                     ttGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCcaaca                                    >  2:4064774/1‑93 (MQ=255)
                                                      tGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCcaacaa                                   <  2:2034095/93‑1 (MQ=255)
                                                         gcATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAAc                                >  2:6383449/1‑93 (MQ=255)
                                                         gcATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAAc                                <  2:2522009/93‑1 (MQ=255)
                                                          cATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAAcc                               <  1:2248018/93‑1 (MQ=255)
                                                            tttCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGa                             >  2:5333451/1‑93 (MQ=255)
                                                              tCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATc                           <  2:1084258/93‑1 (MQ=255)
                                                              tCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATc                           <  1:148751/93‑1 (MQ=255)
                                                               cGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCa                          <  2:2690355/93‑1 (MQ=255)
                                                               cGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCa                          <  2:6270819/93‑1 (MQ=255)
                                                                 tGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAAc                        >  1:3187920/1‑93 (MQ=255)
                                                                   gCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAAcag                      >  2:6656232/1‑93 (MQ=255)
                                                                      gCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAAcagcag                   <  1:2560629/93‑1 (MQ=255)
                                                                         aaTGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGt                <  2:3521705/93‑1 (MQ=255)
                                                                         aaTGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGt                >  2:1937571/1‑93 (MQ=255)
                                                                           tGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTcc              >  1:4363884/1‑93 (MQ=255)
                                                                           tGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTcc              >  2:3754996/1‑93 (MQ=255)
                                                                            gCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTccc             <  2:4771395/93‑1 (MQ=255)
                                                                            gCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTccc             >  2:4475018/1‑93 (MQ=255)
                                                                              aGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAAAAGCAGCGTCCCCa           >  2:1709370/1‑93 (MQ=255)
                                                                              aGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGACACACCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTcccaa           >  2:1133346/1‑91 (MQ=255)
                                                                               gAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAg          >  1:627088/1‑93 (MQ=255)
                                                                               gAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAg          >  1:3992019/1‑93 (MQ=255)
                                                                               gAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAg          >  2:5312547/1‑93 (MQ=255)
                                                                               gAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAg          >  1:1734774/1‑93 (MQ=255)
                                                                                 aCTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCGCCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCTgcg        >  2:4951788/1‑93 (MQ=255)
                                                                                  ctctTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAgcgc       >  1:1547348/1‑93 (MQ=255)
                                                                                   tctTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGcc      >  2:3342646/1‑93 (MQ=255)
                                                                                   tctTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGcc      >  2:6158748/1‑93 (MQ=255)
                                                                                     ttCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAg    <  1:5538965/93‑1 (MQ=255)
                                                                                       cGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGAc  <  1:4385654/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGTATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGAC  >  NC_000913/2296033‑2296212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGACGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGTATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCT  >  NC_000913/2296030‑2296216
                                                                                             |                                                                                             
GGACGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGC                                                                                               >  SRR4302536.6220251/1‑94 (MQ=60)
CGACGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGC                                                                                               <  SRR4302536.6203746/94‑1 (MQ=60)
 GACGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCA                                                                                              >  SRR4302536.6694604/1‑94 (MQ=60)
   CGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATT                                                                                            >  SRR4302536.4825055/1‑94 (MQ=60)
   CGCCGCTACGGTTGTTGTAGTGCACCGAATCCATGTATTCCTGATTCACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAGCTCTTCGGCATT                                                                                            <  SRR4302536.5568588/94‑1 (MQ=60)
     CCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGG                                                                                          <  SRR4302536.271510/94‑1 (MQ=60)
      CGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGC                                                                                         >  SRR4302536.3148789/1‑94 (MQ=60)
       GCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCT                                                                                        >  SRR4302536.4510116/1‑94 (MQ=60)
       GCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCT                                                                                        <  SRR4302536.1570953/94‑1 (MQ=60)
         TACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTT                                                                                      >  SRR4302536.1061720/1‑94 (MQ=60)
         TACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTT                                                                                      <  SRR4302536.4115903/94‑1 (MQ=60)
         TACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTT                                                                                      <  SRR4302536.2068535/94‑1 (MQ=60)
         TACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTT                                                                                      <  SRR4302536.5300889/94‑1 (MQ=60)
         TACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTC                                                                                      <  SRR4302536.3542469/94‑1 (MQ=60)
          ACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTT                                                                                     >  SRR4302536.3208232/1‑94 (MQ=60)
           CGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTT                                                                                    <  SRR4302536.2147061/94‑1 (MQ=60)
           CGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTT                                                                                    <  SRR4302536.1253721/94‑1 (MQ=60)
            GGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTC                                                                                   >  SRR4302536.5099318/1‑94 (MQ=60)
              TTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCA                                                                                 <  SRR4302536.6742446/94‑1 (MQ=60)
               TGTTGTAGTGCACTGAATCCATGTATTCGTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCAT                                                                                <  SRR4302536.2755368/94‑1 (MQ=60)
               TGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCAT                                                                                >  SRR4302536.6220574/1‑94 (MQ=60)
               TGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCAT                                                                                >  SRR4302536.5710644/1‑94 (MQ=60)
                GTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATC                                                                               >  SRR4302536.2834665/1‑94 (MQ=60)
                GTTGTAGTGCACGGAATCCATGAATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATC                                                                               >  SRR4302536.5404175/1‑94 (MQ=60)
                GTTGGAGTGCACCGAATCCCTGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGGTAATGCAGAACTCTTCAGCATTGGCTTTTTCCATC                                                                               >  SRR4302536.5997184/1‑94 (MQ=60)
                  TGTAGTGCACGGAATCCATGTATTCCTGATACCCCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCC                                                                             <  SRR4302536.5000802/94‑1 (MQ=60)
                   GTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCG                                                                            <  SRR4302536.4263654/94‑1 (MQ=60)
                   GTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCG                                                                            <  SRR4302536.1262614/94‑1 (MQ=60)
                    TAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGG                                                                           >  SRR4302536.1972378/1‑94 (MQ=60)
                     AGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCCGT                                                                          >  SRR4302536.2077466/1‑94 (MQ=60)
                      GTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTGCATCCCGGTG                                                                         >  SRR4302536.461453/1‑94 (MQ=60)
                      GTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTG                                                                         >  SRR4302536.5821992/1‑94 (MQ=60)
                      GTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTG                                                                         <  SRR4302536.943769/94‑1 (MQ=60)
                        GCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTT                                                                       >  SRR4302536.1075748/1‑94 (MQ=60)
                         CACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTA                                                                      >  SRR4302536.5986095/1‑94 (MQ=60)
                         CACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTA                                                                      <  SRR4302536.2691003/94‑1 (MQ=60)
                           CGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAA                                                                    <  SRR4302536.1007530/94‑1 (MQ=60)
                           CGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAA                                                                    >  SRR4302536.2112279/1‑94 (MQ=60)
                           CGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAA                                                                    <  SRR4302536.2114576/94‑1 (MQ=60)
                           CGGAATCCATGTATTCCTGATACACCGTGTTGCGCAGTTCGTGGCAGCTAATGCAGATCTCTTCGGTATTGGCTTTTTCCATCCCGGTGTTAAA                                                                    <  SRR4302536.5000795/94‑1 (MQ=60)
                            GGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAG                                                                   <  SRR4302536.216973/94‑1 (MQ=60)
                             GAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGC                                                                  >  SRR4302536.562905/1‑94 (MQ=60)
                              AATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCC                                                                 >  SRR4302536.4647453/1‑94 (MQ=60)
                              AATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCC                                                                 >  SRR4302536.4124921/1‑94 (MQ=60)
                               ATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCA                                                                <  SRR4302536.6391508/94‑1 (MQ=60)
                               ATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCA                                                                <  SRR4302536.5730747/94‑1 (MQ=60)
                               ATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCA                                                                >  SRR4302536.1403506/1‑94 (MQ=60)
                                TCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCAC                                                               >  SRR4302536.858083/1‑94 (MQ=60)
                                  CATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCTGGGGTTAAACCCACCC                                                             >  SRR4302536.1138715/1‑94 (MQ=60)
                                  CATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCC                                                             <  SRR4302536.1680384/94‑1 (MQ=60)
                                  CATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCC                                                             >  SRR4302536.768836/1‑94 (MQ=60)
                                   ATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCAC                                                            >  SRR4302536.2520765/1‑94 (MQ=60)
                                   ATGTATTCCTGATACACCGTGTTGCGCACTTCGTGGCAGGTAATGCAGAAATCTTCGGCATTGGCTTTTTCCATCCCCGTGCTAAAGCCACCAC                                                            >  SRR4302536.4588387/1‑94 (MQ=60)
                                    TGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCA                                                           >  SRR4302536.3982938/1‑94 (MQ=60)
                                       ATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAA                                                        >  SRR4302536.1255848/1‑94 (MQ=60)
                                        TTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAG                                                       <  SRR4302536.5592828/94‑1 (MQ=60)
                                          CCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGAC                                                     <  SRR4302536.1383634/94‑1 (MQ=60)
                                          CCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGAC                                                     >  SRR4302536.3368101/1‑94 (MQ=60)
                                          CCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGAC                                                     >  SRR4302536.5591063/1‑94 (MQ=60)
                                          CCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGAC                                                     <  SRR4302536.2761988/94‑1 (MQ=60)
                                           CTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACG                                                    <  SRR4302536.3880314/94‑1 (MQ=60)
                                            TGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGA                                                   >  SRR4302536.1396598/1‑94 (MQ=60)
                                            TGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGA                                                   >  SRR4302536.5532292/1‑94 (MQ=60)
                                             GATACACCGTGTTGCGCATTACGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGAT                                                  >  SRR4302536.6753266/1‑94 (MQ=60)
                                              ATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATG                                                 >  SRR4302536.6229883/1‑94 (MQ=60)
                                                ACACCGCGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGCGTTAAAGCCACCTCAGAAGACGATGCC                                               >  SRR4302536.2381138/1‑94 (MQ=60)
                                                 CACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCG                                              <  SRR4302536.902861/94‑1 (MQ=60)
                                                 CACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCG                                              <  SRR4302536.2575115/94‑1 (MQ=60)
                                                 CACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCG                                              >  SRR4302536.1265941/1‑94 (MQ=60)
                                                  ACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCAACCCAAGAAGACGATGCCCC                                             >  SRR4302536.579668/1‑94 (MQ=60)
                                                   CCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCC                                            <  SRR4302536.920544/94‑1 (MQ=60)
                                                      TGTTGCGCATTTCGTGGCAGCTAATGCAGACCTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAAC                                         <  SRR4302536.2931133/94‑1 (MQ=60)
                                                       GTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACA                                        <  SRR4302536.1503385/94‑1 (MQ=60)
                                                        TTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAA                                       >  SRR4302536.5374549/1‑94 (MQ=60)
                                                           CGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAAC                                    <  SRR4302536.4139546/94‑1 (MQ=60)
                                                            GCATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACC                                   >  SRR4302536.4637537/1‑94 (MQ=60)
                                                             CATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCG                                  <  SRR4302536.2316065/94‑1 (MQ=60)
                                                             CATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACACCAGAAGACGATGCCGCCAACAAAACCA                                  >  SRR4302536.4529554/1‑94 (MQ=60)
                                                              ATTTCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGA                                 >  SRR4302536.3839220/1‑94 (MQ=60)
                                                                 TCGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCA                              <  SRR4302536.152619/94‑1 (MQ=60)
                                                                  CGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAA                             >  SRR4302536.3011357/1‑94 (MQ=60)
                                                                  CGTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAA                             >  SRR4302536.1986815/1‑94 (MQ=60)
                                                                   GTGGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAAC                            >  SRR4302536.3281388/1‑94 (MQ=60)
                                                                     GGCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAG                          <  SRR4302536.5663274/94‑1 (MQ=60)
                                                                      GCAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGC                         <  SRR4302536.4364546/94‑1 (MQ=60)
                                                                       CAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCA                        >  SRR4302536.3097689/1‑94 (MQ=60)
                                                                       CAGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCA                        <  SRR4302536.89140/94‑1 (MQ=60)
                                                                        AGCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCAGCCAACAAAACCGATCAACAGCAG                       >  SRR4302536.4614154/1‑94 (MQ=60)
                                                                         GCTAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGC                      <  SRR4302536.2637692/94‑1 (MQ=60)
                                                                         GCTAATGCAGAACTCTTCGGCATTGGCTTTTTCAATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGC                      <  SRR4302536.6224527/94‑1 (MQ=60)
                                                                           TAATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGT                    >  SRR4302536.1903626/1‑94 (MQ=60)
                                                                            AATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTC                   >  SRR4302536.827259/1‑94 (MQ=60)
                                                                             ATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCC                  <  SRR4302536.255315/94‑1 (MQ=60)
                                                                             ATGCAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCC                  >  SRR4302536.4494292/1‑94 (MQ=60)
                                                                                CAGAACTCTTCGGCATTGGCTTTTTCTATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCA               >  SRR4302536.477245/1‑94 (MQ=60)
                                                                                CAGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGTCGCCAACAAAACCGATCAACAGCAGCGTCCCCA               >  SRR4302536.2078054/1‑94 (MQ=60)
                                                                                 AGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAG              >  SRR4302536.4110494/1‑94 (MQ=60)
                                                                                 AGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAG              >  SRR4302536.1784812/1‑94 (MQ=60)
                                                                                 AGAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAG              >  SRR4302536.645681/1‑94 (MQ=60)
                                                                                  GAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGC             >  SRR4302536.5742011/1‑94 (MQ=60)
                                                                                  GAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGC             <  SRR4302536.6885410/94‑1 (MQ=60)
                                                                                  GAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGC             <  SRR4302536.5833247/94‑1 (MQ=60)
                                                                                  GAACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCACCGTCCCCAGC             >  SRR4302536.5054203/1‑94 (MQ=60)
                                                                                    ACTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGC           >  SRR4302536.1592831/1‑94 (MQ=60)
                                                                                     CTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCC          >  SRR4302536.6401660/1‑94 (MQ=60)
                                                                                     CTCTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAAACGATCAACAGCAGCGTCCCCAGAGCC          >  SRR4302536.1718838/1‑94 (MQ=60)
                                                                                       CTTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAG        >  SRR4302536.2626814/1‑94 (MQ=60)
                                                                                        TTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGA       <  SRR4302536.5704040/94‑1 (MQ=60)
                                                                                        TTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGA       >  SRR4302536.2901786/1‑94 (MQ=60)
                                                                                        TTCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGA       >  SRR4302536.4074500/1‑94 (MQ=60)
                                                                                         TCGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGAC      >  SRR4302536.2749183/1‑94 (MQ=60)
                                                                                          CGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACG     <  SRR4302536.4516615/94‑1 (MQ=60)
                                                                                          CGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACG     <  SRR4302536.6090992/94‑1 (MQ=60)
                                                                                          CGGCATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACG     >  SRR4302536.2175013/1‑94 (MQ=60)
                                                                                             CATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCT  <  SRR4302536.1965431/94‑1 (MQ=60)
                                                                                             CATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCT  >  SRR4302536.5294152/1‑94 (MQ=60)
                                                                                             |                                                                                             
GGACGCCGCTACGGTTGTTGTAGTGCACGGAATCCATGTATTCCTGATACACCGTGTTGCGCATTTCGTGGCAGCTAATGCAGAACTCTTCGGTATTGGCTTTTTCCATCCCGGTGTTAAAGCCACCCCAGAAGACGATGCCGCCAACAAAACCGATCAACAGCAGCGTCCCCAGCGCCAGACGGCT  >  NC_000913/2296030‑2296216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑