Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,321,092 G→A A403T (GCC→ACC)  atoC → ornithine decarboxylase antizyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,321,0920GA97.9% 623.6 / ‑1.0 195A403T (GCC→ACC) atoCornithine decarboxylase antizyme
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (87/101);  total (89/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01

Only 100 of 206 total aligned reads displayed.

GTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAAC  >  NC_000913/2321002‑2321181
                                                                                          |                                                                                         
gtcgtGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAAcc                                                                                         <  1:2555974/93‑1 (MQ=255)
 tcgtGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAAccc                                                                                        <  1:3772029/93‑1 (MQ=255)
  cgtGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAAcccc                                                                                       <  2:1604126/93‑1 (MQ=255)
   gtGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCt                                                                                      >  1:1317989/1‑93 (MQ=255)
   gtGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCt                                                                                      >  1:1717003/1‑93 (MQ=255)
    tgatgaATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTg                                                                                     >  1:4431590/1‑93 (MQ=255)
    tgatgaATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTg                                                                                     <  1:753455/93‑1 (MQ=255)
     gatgaATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGt                                                                                    <  2:1175240/93‑1 (MQ=255)
     gatgaATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGt                                                                                    <  1:1843889/93‑1 (MQ=255)
     gatgaATTCAGGCATGACATTTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGt                                                                                    <  1:4032732/93‑1 (MQ=255)
       tgaATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCg                                                                                  <  1:1104015/93‑1 (MQ=255)
        gaATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCgg                                                                                 <  1:2722220/93‑1 (MQ=255)
        gaATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCAACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCgg                                                                                 >  2:1067427/1‑93 (MQ=255)
          aTTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTg                                                                               <  1:1909662/93‑1 (MQ=255)
           ttCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGa                                                                              <  2:469737/93‑1 (MQ=255)
             cAGGCCCGATCATTTTTTCTGAGGACCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGc                                                                            <  2:617510/93‑1 (MQ=255)
                  ccGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAt                                                                       <  1:1289158/93‑1 (MQ=255)
                  ccGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAt                                                                       >  1:715544/1‑93 (MQ=255)
                   cGATCTGTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAtt                                                                      <  1:2558843/93‑1 (MQ=255)
                   cGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAtt                                                                      >  1:4457457/1‑93 (MQ=255)
                   cGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAtt                                                                      >  1:540466/1‑93 (MQ=255)
                   cGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAtt                                                                      >  2:2835671/1‑93 (MQ=255)
                   cGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAtt                                                                      <  2:4074627/93‑1 (MQ=255)
                   cGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAtt                                                                      >  1:922894/1‑93 (MQ=255)
                     aTCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTa                                                                    >  1:1516537/1‑93 (MQ=255)
                      tCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTaa                                                                   >  2:2482181/1‑93 (MQ=255)
                        aTTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAACGCTGGCGAGGTAAAAATAACTTCTGTCGGGGAGCGTAATTTaaaa                                                                 >  1:4227090/1‑93 (MQ=255)
                          tttgtCTGAGGAGCTGCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAaga                                                               <  1:2026685/93‑1 (MQ=255)
                           ttttCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAagag                                                              <  2:1664706/93‑1 (MQ=255)
                             ttCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGa                                                            <  1:4120792/93‑1 (MQ=255)
                              tCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGaa                                                           <  2:1912290/93‑1 (MQ=255)
                                  aGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTa                                                       >  1:3876071/1‑93 (MQ=255)
                                   ggATCTCCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTaa                                                      <  2:583335/93‑1 (MQ=255)
                                    gATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGTGTAATTTAAAAGAGGAAATTaaa                                                     >  2:2584062/1‑93 (MQ=255)
                                     aTCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAAc                                                    <  1:681729/93‑1 (MQ=255)
                                      tCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAAcg                                                   <  1:4428060/93‑1 (MQ=255)
                                        ttCCGCCACAGTTATACCAGACAGTCTGTAATGCTGGCGAGGTAAAAACATCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAAcgcg                                                 <  2:3562401/93‑1 (MQ=255)
                                        ttCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAAcgcg                                                 >  2:2245627/1‑93 (MQ=255)
                                        ttCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAAcgcg                                                 >  1:624868/1‑93 (MQ=255)
                                           cGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCg                                              <  1:3460976/93‑1 (MQ=255)
                                           cGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCg                                              <  1:3060041/93‑1 (MQ=255)
                                            gCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGa                                             >  1:1955198/1‑93 (MQ=255)
                                            cccACAGATTCGCCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGa                                             <  2:3916672/92‑1 (MQ=255)
                                              cacaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaaa                                           >  2:1359575/1‑93 (MQ=255)
                                              cacaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaaa                                           <  1:4372030/93‑1 (MQ=255)
                                               acaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaaaa                                          >  2:1979906/1‑93 (MQ=255)
                                               acaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaaaa                                          >  2:3513495/1‑93 (MQ=255)
                                               acaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaaaa                                          >  2:2778696/1‑93 (MQ=255)
                                                caGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTTGaaaaa                                         <  2:4606773/93‑1 (MQ=255)
                                                caGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaaaaa                                         <  1:1608644/93‑1 (MQ=255)
                                                caGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaaaaa                                         <  2:434619/93‑1 (MQ=255)
                                                caGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaaaaa                                         <  1:2313474/93‑1 (MQ=255)
                                                 aGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAAc                                        >  1:3184584/1‑93 (MQ=255)
                                                 aGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGGAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAAc                                        >  2:536250/1‑93 (MQ=255)
                                                       gTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGcatca                                  <  2:2654825/93‑1 (MQ=255)
                                                       gTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGcatca                                  >  1:873239/1‑93 (MQ=255)
                                                       gTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGcatca                                  <  2:3738826/93‑1 (MQ=255)
                                                       gTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGcatca                                  >  1:795606/1‑93 (MQ=255)
                                                       gTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGcatca                                  <  2:785643/93‑1 (MQ=255)
                                                        tCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGcatcat                                 >  1:2167739/1‑93 (MQ=255)
                                                        tCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGcatcat                                 >  1:1215267/1‑93 (MQ=255)
                                                         cAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCAtt                                <  1:957219/93‑1 (MQ=255)
                                                         cAGCCAGGCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCAtt                                <  1:372881/93‑1 (MQ=255)
                                                           gCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTAt                              <  2:3252429/93‑1 (MQ=255)
                                                            ccAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATg                             <  2:3124983/93‑1 (MQ=255)
                                                             cAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTTTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATgg                            <  1:3354433/93‑1 (MQ=255)
                                                              aGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGa                           >  1:1470702/1‑93 (MQ=255)
                                                              aGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGa                           >  1:3982468/1‑93 (MQ=255)
                                                              aGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGa                           <  1:2408400/93‑1 (MQ=255)
                                                               gTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGaa                          <  1:583450/93‑1 (MQ=255)
                                                               gTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGaa                          >  2:3076278/1‑93 (MQ=255)
                                                                tCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAg                         <  2:2336014/93‑1 (MQ=255)
                                                                 cTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGt                        <  1:4677662/93‑1 (MQ=255)
                                                                  tGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGtt                       >  1:2263070/1‑92 (MQ=255)
                                                                   gTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGc                      >  1:4242280/1‑93 (MQ=255)
                                                                     aaTGCTGGCGAGGTAAAAACATCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTg                    <  2:888299/93‑1 (MQ=255)
                                                                     aaTGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTg                    >  2:1499092/1‑93 (MQ=255)
                                                                      aTGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTgg                   >  1:2126424/1‑93 (MQ=255)
                                                                      aTGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTga                   >  1:2366208/1‑92 (MQ=255)
                                                                       tGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGa                  >  2:1682388/1‑93 (MQ=255)
                                                                       tGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGa                  >  2:84058/1‑93 (MQ=255)
                                                                        gCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGaa                 >  1:1197819/1‑93 (MQ=255)
                                                                          tGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGaaca               >  2:2150839/1‑93 (MQ=255)
                                                                          tGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGaaca               >  1:1375748/1‑93 (MQ=255)
                                                                           ggCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGaacaa              >  2:4236230/1‑93 (MQ=255)
                                                                            gCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGaacaac             <  1:2715615/93‑1 (MQ=255)
                                                                             cGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGGATCATTATGGAAGTGCTGGAacaaca            <  2:3811833/93‑1 (MQ=255)
                                                                              gAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAAcaacaa           >  2:2194296/1‑93 (MQ=255)
                                                                              gAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAAcaacaa           <  2:4368593/93‑1 (MQ=255)
                                                                               aGGTAAAAACAACCTCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACaag          <  1:356168/93‑1 (MQ=255)
                                                                               aGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACaag          <  1:2486550/93‑1 (MQ=255)
                                                                                 gTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACaagaa        >  2:3079860/1‑93 (MQ=255)
                                                                                 gTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACaagaa        <  1:1278187/93‑1 (MQ=255)
                                                                                 gTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACaagaa        <  1:3291792/93‑1 (MQ=255)
                                                                                   aaaaaCAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAgg      <  2:682469/93‑1 (MQ=255)
                                                                                    aaaaCAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGa     <  1:1331347/93‑1 (MQ=255)
                                                                                    aaaaCAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGa     <  2:113207/93‑1 (MQ=255)
                                                                                     aaaCAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGaa    >  1:1006207/1‑93 (MQ=255)
                                                                                      aacaacCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGaaa   <  2:2538786/93‑1 (MQ=255)
                                                                                       aCAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAAc  <  2:2704945/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAAC  >  NC_000913/2321002‑2321181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGA  >  NC_000913/2321000‑2321184
                                                                                            |                                                                                            
CCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAAC                                                                                             <  SRR4302544.4584194/94‑1 (MQ=60)
  GTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCC                                                                                           <  SRR4302544.2580640/94‑1 (MQ=60)
  GTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCC                                                                                           <  SRR4302544.2580515/94‑1 (MQ=60)
  GTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCAACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCC                                                                                           >  SRR4302544.3856981/1‑94 (MQ=60)
   TCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCC                                                                                          <  SRR4302544.3809415/94‑1 (MQ=60)
    CGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCT                                                                                         >  SRR4302544.3084695/1‑94 (MQ=60)
    CGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCT                                                                                         >  SRR4302544.1733882/1‑94 (MQ=60)
    CGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCT                                                                                         >  SRR4302544.1330947/1‑94 (MQ=60)
     GTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTG                                                                                        >  SRR4302544.4476299/1‑94 (MQ=60)
      TGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGT                                                                                       <  SRR4302544.760812/94‑1 (MQ=60)
       gatgaattcaggcatgacatTTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTC                                                                                      <  SRR4302544.4072903/74‑1 (MQ=60)
       GATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTC                                                                                      <  SRR4302544.1861759/94‑1 (MQ=60)
       GATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTC                                                                                      <  SRR4302544.3772666/94‑1 (MQ=60)
        ATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCG                                                                                     <  SRR4302544.4007014/94‑1 (MQ=60)
         TGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGG                                                                                    <  SRR4302544.1115009/94‑1 (MQ=60)
          GAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGT                                                                                   <  SRR4302544.2748429/94‑1 (MQ=60)
           AATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTG                                                                                  >  SRR4302544.2541624/1‑94 (MQ=60)
            ATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGA                                                                                 <  SRR4302544.1928119/94‑1 (MQ=60)
              TCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGC                                                                               <  SRR4302544.1344795/94‑1 (MQ=60)
                  GCCCGATCATTTTTTCGGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAA                                                                           <  SRR4302544.1482713/94‑1 (MQ=60)
                   CCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAT                                                                          <  SRR4302544.4046329/94‑1 (MQ=60)
                   CCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAT                                                                          >  SRR4302544.722617/1‑94 (MQ=60)
                    CCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATT                                                                         >  SRR4302544.2171477/1‑94 (MQ=60)
                    CCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATT                                                                         >  SRR4302544.4502338/1‑94 (MQ=60)
                    CCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATT                                                                         <  SRR4302544.3597291/94‑1 (MQ=60)
                    CCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATT                                                                         >  SRR4302544.1032386/1‑94 (MQ=60)
                    CCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATT                                                                         <  SRR4302544.1301855/94‑1 (MQ=60)
                    CCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATT                                                                         >  SRR4302544.931992/1‑94 (MQ=60)
                    CCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATT                                                                         >  SRR4302544.545791/1‑94 (MQ=60)
                     ctttttttcttctcgaggaccttccgccctaggTTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTT                                                                        <  SRR4302544.3786581/61‑1 (MQ=60)
                     CGATCTGTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTT                                                                        <  SRR4302544.2583420/94‑1 (MQ=60)
                     CGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGACATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTT                                                                        >  SRR4302544.2742431/1‑94 (MQ=60)
                      GATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTA                                                                       >  SRR4302544.1531583/1‑94 (MQ=60)
                         CATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAACGCTGGCGAGGTAAAAATAACTTCTGTCGGGGAGCGTAATTTAAAA                                                                    >  SRR4302544.4270136/1‑94 (MQ=60)
                            TTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAG                                                                 >  SRR4302544.896429/1‑94 (MQ=60)
                            TTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAG                                                                 <  SRR4302544.2238331/94‑1 (MQ=60)
                            TTTGTCTGAGGAGCTGCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAG                                                                 <  SRR4302544.2046326/94‑1 (MQ=60)
                             TTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGG                                                                <  SRR4302544.192919/94‑1 (MQ=60)
                              TTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGA                                                               <  SRR4302544.1789546/94‑1 (MQ=60)
                               TTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAA                                                              <  SRR4302544.4161692/94‑1 (MQ=60)
                               TTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAA                                                              >  SRR4302544.2057491/1‑94 (MQ=60)
                               TTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAA                                                              >  SRR4302544.529006/1‑94 (MQ=60)
                               TTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAA                                                              <  SRR4302544.760931/94‑1 (MQ=60)
                                TCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAA                                                             >  SRR4302544.3756210/1‑94 (MQ=60)
                                 TTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAAT                                                            <  SRR4302544.71223/94‑1 (MQ=60)
                                 CTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAAT                                                            <  SRR4302544.562139/94‑1 (MQ=60)
                                   GAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTA                                                          >  SRR4302544.3914559/1‑94 (MQ=60)
                                      GATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAAC                                                       >  SRR4302544.933487/1‑94 (MQ=60)
                                       ATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACG                                                      <  SRR4302544.688505/94‑1 (MQ=60)
                                        TCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGC                                                     <  SRR4302544.4472738/94‑1 (MQ=60)
                                         CTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCG                                                    >  SRR4302544.631007/1‑94 (MQ=60)
                                         CTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCG                                                    >  SRR4302544.631008/1‑94 (MQ=60)
                                            CCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCG                                                 <  SRR4302544.128830/94‑1 (MQ=60)
                                            CCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCG                                                 <  SRR4302544.3473588/94‑1 (MQ=60)
                                             CGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGA                                                >  SRR4302544.4677140/1‑94 (MQ=60)
                                             CGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGA                                                <  SRR4302544.3089242/94‑1 (MQ=60)
                                             CGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGA                                                <  SRR4302544.3494009/94‑1 (MQ=60)
                                             CGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGA                                                >  SRR4302544.1974160/1‑94 (MQ=60)
                                                CACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAA                                             <  SRR4302544.4416355/94‑1 (MQ=60)
                                                  CAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAAC                                           >  SRR4302544.3214897/1‑94 (MQ=60)
                                                  CAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAAC                                           <  SRR4302544.1624504/94‑1 (MQ=60)
                                                  CAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAAC                                           <  SRR4302544.2335359/94‑1 (MQ=60)
                                                   AGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAATAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACG                                          <  SRR4302544.4103457/94‑1 (MQ=60)
                                                   AGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACG                                          <  SRR4302544.1488636/94‑1 (MQ=60)
                                                     ATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCA                                        >  SRR4302544.3347506/1‑94 (MQ=60)
                                                      TTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCAT                                       <  SRR4302544.423643/94‑1 (MQ=60)
                                                       tctgCAGCCAGTCGGTAATGCTGGCGACGTAAAAACAACCCCTGTTGGTGAGCTTAATTTAAAAGAGGAAATTAACCGCGTCGAAAAACGCATC                                      <  SRR4302544.2450629/90‑1 (MQ=60)
                                                       TCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATC                                      >  SRR4302544.726369/1‑94 (MQ=60)
                                                        CGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCA                                     >  SRR4302544.881901/1‑94 (MQ=60)
                                                        CGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCA                                     >  SRR4302544.803313/1‑94 (MQ=60)
                                                         GTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCAT                                    >  SRR4302544.1227295/1‑94 (MQ=60)
                                                         GTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCAT                                    >  SRR4302544.2188308/1‑94 (MQ=60)
                                                           CAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTA                                  <  SRR4302544.966627/94‑1 (MQ=60)
                                                           CAGCCAGGCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTA                                  <  SRR4302544.376692/94‑1 (MQ=60)
                                                            AGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTAT                                 <  SRR4302544.4339267/94‑1 (MQ=60)
                                                            AGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTAT                                 >  SRR4302544.4459549/1‑94 (MQ=60)
                                                              CCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGG                               <  SRR4302544.3545742/94‑1 (MQ=60)
                                                               CAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTTTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGA                              <  SRR4302544.3386442/94‑1 (MQ=60)
                                                               CAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGA                              >  SRR4302544.1485273/1‑94 (MQ=60)
                                                               CAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGA                              >  SRR4302544.4022103/1‑94 (MQ=60)
                                                                AGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAA                             <  SRR4302544.1541527/94‑1 (MQ=60)
                                                                AGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAA                             >  SRR4302544.1223969/1‑94 (MQ=60)
                                                                AGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAA                             <  SRR4302544.2431281/94‑1 (MQ=60)
                                                                AGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGGGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAA                             <  SRR4302544.2431280/94‑1 (MQ=60)
                                                                 GTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAG                            <  SRR4302544.1125357/94‑1 (MQ=60)
                                                                 GTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAG                            <  SRR4302544.589172/94‑1 (MQ=60)
                                                                   CTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTT                          >  SRR4302544.2284354/1‑94 (MQ=60)
                                                                   CTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTG                          <  SRR4302544.4723906/94‑1 (MQ=60)
                                                                   CTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTG                          <  SRR4302544.4488831/94‑1 (MQ=60)
                                                                   CTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTG                          >  SRR4302544.678062/1‑94 (MQ=60)
                                                                    TGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGC                         >  SRR4302544.4285524/1‑94 (MQ=60)
                                                                    TGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGC                         >  SRR4302544.3283080/1‑94 (MQ=60)
                                                                       AATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGG                      >  SRR4302544.2146813/1‑94 (MQ=60)
                                                                       AATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGA                      >  SRR4302544.2388598/1‑94 (MQ=60)
                                                                         TGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAA                    <  SRR4302544.427290/94‑1 (MQ=60)
                                                                         TGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAA                    >  SRR4302544.1209692/1‑94 (MQ=60)
                                                                           CTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACA                  >  SRR4302544.1389461/1‑94 (MQ=60)
                                                                              GCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACA               <  SRR4302544.2741751/94‑1 (MQ=60)
                                                                                 AGGTAAAAACAACCTCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGA            <  SRR4302544.359820/94‑1 (MQ=60)
                                                                                 AGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGA            <  SRR4302544.2510370/94‑1 (MQ=60)
                                                                                   GTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAG          <  SRR4302544.1290772/94‑1 (MQ=60)
                                                                                   GTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAG          <  SRR4302544.3323153/94‑1 (MQ=60)
                                                                                   GTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAG          <  SRR4302544.2622889/94‑1 (MQ=60)
                                                                                     AAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGA        >  SRR4302544.163940/1‑94 (MQ=60)
                                                                                      AAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAA       <  SRR4302544.2335462/94‑1 (MQ=60)
                                                                                      AAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAA       <  SRR4302544.1344446/94‑1 (MQ=60)
                                                                                      AAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAA       <  SRR4302544.1344443/94‑1 (MQ=60)
                                                                                      AAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAA       >  SRR4302544.1291819/1‑94 (MQ=60)
                                                                                      AAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAA       >  SRR4302544.1016211/1‑94 (MQ=60)
                                                                                       AAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAA      >  SRR4302544.1976965/1‑94 (MQ=60)
                                                                                       AAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAA      <  SRR4302544.4473905/94‑1 (MQ=60)
                                                                                       AAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAA      <  SRR4302544.678720/94‑1 (MQ=60)
                                                                                       AAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAA      >  SRR4302544.2309449/1‑94 (MQ=60)
                                                                                        AACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAAC     <  SRR4302544.3147402/94‑1 (MQ=60)
                                                                                        AACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAAC     <  SRR4302544.855116/94‑1 (MQ=60)
                                                                                         ACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACC    >  SRR4302544.2481279/1‑94 (MQ=60)
                                                                                         ACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACC    <  SRR4302544.2115795/94‑1 (MQ=60)
                                                                                         ACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACC    <  SRR4302544.1307437/94‑1 (MQ=60)
                                                                                         ACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACC    <  SRR4302544.1923891/94‑1 (MQ=60)
                                                                                          CAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCG   <  SRR4302544.3324016/94‑1 (MQ=60)
                                                                                          CAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCG   >  SRR4302544.2337230/1‑94 (MQ=60)
                                                                                           AACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGA  >  SRR4302544.3403304/1‑94 (MQ=60)
                                                                                           AACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGA  >  SRR4302544.3357435/1‑94 (MQ=60)
                                                                                           AACCCCTGTCGGGGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACAGA  >  SRR4302544.3012248/1‑94 (MQ=60)
                                                                                            |                                                                                            
CCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGA  >  NC_000913/2321000‑2321184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑