Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,324,469	(G)_6→7	coding (341/1185 nt)	atoB →	acetyl‑CoA acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,324,463	1	.	G	98.9%	289.7 / ‑3.4	91	coding (335/1185 nt)	atoB	acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (34/56); total (35/56)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.45e-01

Only 100 of 129 total aligned reads displayed.

TCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTA  >  NC_000913/2324373‑2324550

tCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑gg                                                                                       <  2:1482172/93‑1 (MQ=255)

tCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑gg                                                                                       <  1:2218396/93‑1 (MQ=255)

tCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑gg                                                                                       >  1:3809413/1‑93 (MQ=255)

 cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCGCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑ggg                                                                                      <  1:3361701/93‑1 (MQ=255)

  aCGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCATGCGCAGAGCATTGTGGC‑‑gggg                                                                                     <  2:1885636/93‑1 (MQ=255)

  aCGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑gggg                                                                                     <  1:4381870/93‑1 (MQ=255)

   cGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGGCGCCCAGGGCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑ggggg                                                                                    >  1:2116390/1‑93 (MQ=255)

   cGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑ggggg                                                                                    <  1:2598153/93‑1 (MQ=255)

   cGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑ggggg                                                                                    <  1:1717257/93‑1 (MQ=255)

   cGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑ggggg                                                                                    >  2:1615478/1‑93 (MQ=255)

    ggTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTTGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGGATTGTGgg‑‑gggggg                                                                                   >  1:1846021/1‑93 (MQ=255)

    ggTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑gggggg                                                                                   >  2:2443037/1‑93 (MQ=255)

     gtaaaTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑ggggggg                                                                                  >  1:1022986/4‑92 (MQ=255)

     gTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑ggggggg                                                                                  >  2:4036074/1‑92 (MQ=255)

     gTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑ggggggg                                                                                  <  1:1087505/93‑2 (MQ=255)

        aaTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑gggggggtat                                                                               <  2:252326/93‑5 (MQ=255)

             aGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGaaa                                                                           >  2:4572809/1‑93 (MQ=255)

             aGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGaaa                                                                           >  2:131648/1‑93 (MQ=255)

             aGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGGGGGATGGgaaa                                                                          >  1:3047256/1‑93 (MQ=255)

              gTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGaaaa                                                                          <  1:1323294/93‑1 (MQ=255)

               tATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAat                                                                         <  2:3037628/93‑1 (MQ=255)

                   tgGTTCGGGTCTTAAAAGTGTGGCTCTTGCCGCCCAGGCCATGCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGa                                                                     <  2:3137895/93‑1 (MQ=255)

                   tgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGa                                                                     <  1:2491263/93‑1 (MQ=255)

                   tgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGa                                                                     >  1:3862182/1‑93 (MQ=255)

                      ttCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGtt                                                                  <  2:3084658/93‑1 (MQ=255)

                      ttCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGtt                                                                  <  1:4045853/93‑1 (MQ=255)

                       tCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGttt                                                                 <  1:790995/93‑1 (MQ=255)

                       tCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGttt                                                                 <  1:790994/93‑1 (MQ=255)

                       tCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGttt                                                                 <  2:3210913/93‑1 (MQ=255)

                        cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTa                                                                <  2:827719/93‑1 (MQ=255)

                        cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGGAAATATGAGTTTa                                                                >  2:1569332/1‑93 (MQ=255)

                         gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGAGTTTAg                                                               >  1:3633020/1‑93 (MQ=255)

                          ggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGc                                                              <  1:1183269/93‑1 (MQ=255)

                          ggTCTTAAAAGTGTGGCGCTTGCAGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGc                                                              <  2:290571/93‑1 (MQ=255)

                            tCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGccc                                                            >  2:3785424/1‑93 (MQ=255)

                             cTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGcccc                                                           <  2:197115/93‑1 (MQ=255)

                             cTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGAGGTTAGcccc                                                           >  1:2868231/1‑93 (MQ=255)

                              ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGccccc                                                          <  2:3045680/93‑1 (MQ=255)

                              ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGccccc                                                          <  1:1860700/93‑1 (MQ=255)

                               tAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCt                                                         <  2:3052260/93‑1 (MQ=255)

                                aaaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCtc                                                        >  1:3153924/1‑92 (MQ=255)

                                 aaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCtac                                                       <  2:2128493/93‑1 (MQ=255)

                                 aaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGGTGGGAAATATTAGGTTATCCCCCtac                                                       >  2:3423442/1‑93 (MQ=255)

                                 aaaGTGTGGCGCTTGCAGCCCAGGCCATTCAGGTAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCtac                                                       <  1:4737199/93‑1 (MQ=255)

                                  aaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCtact                                                      >  2:1465300/1‑93 (MQ=255)

                                  aaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGAGGTTAGCCCCCtact                                                      >  2:3124702/1‑93 (MQ=255)

                                   aGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGGAAATATGAGTTTAGCCCCCtactt                                                     >  2:94260/1‑93 (MQ=255)

                                    gtgtGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCtactta                                                    <  2:3253228/93‑1 (MQ=255)

                                    gtgtGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGGTGGAAAATATGAGTTTAGCCCCCtactta                                                    >  2:891956/1‑93 (MQ=255)

                                     tgtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCtacttac                                                   <  1:3930182/93‑1 (MQ=255)

                                     tgtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCtacttac                                                   <  1:1322446/93‑1 (MQ=255)

                                     tgtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGCG‑‑GGGGGGGATGGGAAATATGGGGTTTGGCCCCtacttact                                                  >  2:4703881/1‑93 (MQ=255)

                                      gtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGGAAATATGAGTTTAGCCCCCtacttact                                                  >  2:4491780/1‑93 (MQ=255)

                                      gtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGGCCCCaactttct                                                  >  2:2919781/1‑93 (MQ=255)

                                       tgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTc                                                 <  1:2398248/93‑1 (MQ=255)

                                         gcgcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGa                                               <  1:4467590/93‑1 (MQ=255)

                                          ggcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGAt                                              <  1:2617266/92‑1 (MQ=255)

                                            cTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGc                                            <  1:3960444/93‑1 (MQ=255)

                                                ccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCaaaa                                        <  2:1442547/93‑1 (MQ=255)

                                                ccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCaaaa                                        <  1:3764553/93‑1 (MQ=255)

                                                ccccccAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGGGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCaaaa                                        <  1:2056410/90‑1 (MQ=255)

                                                     cAGGCCATTCAGTCATGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACg                                   <  1:896375/93‑1 (MQ=255)

                                                     cAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACg                                   <  1:1821958/93‑1 (MQ=255)

                                                     cAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGGAAATATGAGTTTAGCCCCCTACTTATTCGATGCAAAAGAACg                                   >  1:1057746/1‑93 (MQ=255)

                                                     cAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGGAAACATGAGGTTAGGCCCCAACTTACCCGATGCAAAAGCaag                                   >  2:1820627/1‑91 (MQ=255)

                                                     cAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACg                                   >  2:3064870/1‑93 (MQ=255)

                                                      aGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGc                                  <  1:2216855/93‑1 (MQ=255)

                                                       ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGGATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGAAAAAGCACGc                                  >  1:3983712/1‑93 (MQ=255)

                                                       ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGCACGct                                 >  2:2531303/1‑93 (MQ=255)

                                                        gCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGctc                                <  1:4462065/93‑1 (MQ=255)

                                                        gCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGctc                                <  1:4462066/93‑1 (MQ=255)

                                                        gCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGctc                                <  2:1263190/93‑1 (MQ=255)

                                                        gCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGCACGctc                                >  2:780207/1‑93 (MQ=255)

                                                         ccATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGCACGctct                               >  1:4143412/1‑93 (MQ=255)

                                                          cATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTg                              <  2:394901/93‑1 (MQ=255)

                                                          cATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTgg                             >  2:4527932/1‑93 (MQ=255)

                                                            ttCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGt                            >  2:947185/1‑93 (MQ=255)

                                                                ggcaggTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATc                        <  1:2328697/93‑1 (MQ=255)

                                                                ggcaggTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACCCTCTGGTTATc                        >  1:909958/1‑93 (MQ=255)

                                                                  caggTCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGt                      >  2:2989566/1‑93 (MQ=255)

                                                                   aggTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATTGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACCCTCTGGTTATCGTc                     >  2:1989667/1‑93 (MQ=255)

                                                                   aggTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTc                     <  2:1428748/93‑1 (MQ=255)

                                                                   aggTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTc                     >  1:4596525/1‑93 (MQ=255)

                                                                    ggTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCt                    <  2:960214/93‑1 (MQ=255)

                                                                     gTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCtt                   <  2:1914822/93‑1 (MQ=255)

                                                                     gTCAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCtt                   <  2:1833509/93‑1 (MQ=255)

                                                                      tCAGGCGCAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTg                  >  2:4146712/1‑93 (MQ=255)

                                                                       cAGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTgg                 <  1:4742225/93‑1 (MQ=255)

                                                                        aGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGga                <  1:3916306/93‑1 (MQ=255)

                                                                        aGGCGCAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAACACGCTCTGGTTATCGTCTTTga                >  2:2664526/1‑93 (MQ=255)

                                                                           cgcAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGAc             <  1:3267814/93‑1 (MQ=255)

                                                                            gcAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACg            >  2:3568028/1‑93 (MQ=255)

                                                                            gcAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACg            >  1:2866515/1‑93 (MQ=255)

                                                                            gcAGAGCATTGTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACg            >  2:3271233/1‑93 (MQ=255)

                                                                            gcAGAGCATTGGGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACg            <  1:1296134/93‑1 (MQ=255)

                                                                             cAGAGCATTGTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACgg           <  2:1559649/93‑1 (MQ=255)

                                                                              agagCATTGTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGa          >  1:2121371/1‑93 (MQ=255)

                                                                               gagCATTGTGGCG‑GGGGGGGATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGTACGCTCTGGGTATCGTCTTGGAGACGGAc         >  2:419060/1‑93 (MQ=255)

                                                                                      gtgGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTa  <  1:3305190/93‑1 (MQ=255)

                                                                                      gtgGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGGGACGGACAGGTTTa  >  2:1439665/1‑93 (MQ=255)

TCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTA  >  NC_000913/2324373‑2324550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  >  NC_000913/2324376‑2324562

CGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTTGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGGATTGTGGG‑‑GGGG‑GG                                                                                               >  SRR4302544.1863903/1‑94 (MQ=60)

CGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑GG                                                                                               <  SRR4302544.1734141/94‑1 (MQ=60)

CGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑GG                                                                                               <  SRR4302544.2623130/94‑1 (MQ=60)

 GGTAAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑GGG                                                                                              >  SRR4302544.1033203/1‑94 (MQ=60)

  GTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑GGgt                                                                                             <  SRR4302544.2865039/94‑3 (MQ=60)

  GTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑GGgt                                                                                             <  SRR4302544.1098318/94‑3 (MQ=60)

    CAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑GGggag                                                                                           >  SRR4302544.4563485/1‑90 (MQ=60)

     AATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑GGggatg                                                                                          >  SRR4302544.825087/1‑89 (MQ=60)

     AATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑GGggacg                                                                                          >  SRR4302544.825086/1‑89 (MQ=60)

     AATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGAC‑‑GGGG‑GGggatg                                                                                          >  SRR4302544.825085/1‑89 (MQ=60)

       TAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑GGggatgga                                                                                        >  SRR4302544.496097/1‑87 (MQ=60)

       TAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑GGggatgga                                                                                        >  SRR4302544.2181313/1‑87 (MQ=60)

         AAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑GGGATGGGAAA                                                                                      >  SRR4302544.3076384/1‑94 (MQ=60)

           GTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAAT                                                                                     <  SRR4302544.1336311/94‑1 (MQ=60)

           GTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑GGggatgggaaaa                                                                                    >  SRR4302544.4390039/1‑83 (MQ=60)

               GTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGGATGGAAAATATGA                                                                                 >  SRR4302544.3900526/1‑94 (MQ=60)

                TGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAG                                                                                <  SRR4302544.2515123/94‑1 (MQ=60)

                   TTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTT                                                                             <  SRR4302544.4086153/94‑1 (MQ=60)

                    TCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTA                                                                            <  SRR4302544.798652/94‑1 (MQ=60)

                    TCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTA                                                                            <  SRR4302544.798651/94‑1 (MQ=60)

                     CGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAG                                                                           <  SRR4302544.2494578/94‑1 (MQ=60)

                     CGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGGATGGGAAATATGAGTTTAG                                                                           >  SRR4302544.4001149/1‑94 (MQ=60)

                     CGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGGATGGAAAATATGAGTTTAG                                                                           >  SRR4302544.3669243/1‑94 (MQ=60)

                      GGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGC                                                                          <  SRR4302544.1606896/94‑1 (MQ=60)

                       GGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCC                                                                         <  SRR4302544.1195037/94‑1 (MQ=60)

                         TCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGGATGGAAAATATGAGGTTAGCCCC                                                                       >  SRR4302544.2895919/1‑94 (MQ=60)

                           TTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCT                                                                     <  SRR4302544.1878688/94‑1 (MQ=60)

                            TAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGGATGGAAAATATGAGTTTAGCCCCCTC                                                                    >  SRR4302544.3183923/1‑94 (MQ=60)

                              AAAGTGTGGCGCTTGCAGCCCAGGCCATTCAGGTAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACT                                                                  <  SRR4302544.4783897/94‑1 (MQ=60)

                                  TGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACT                                                              <  SRR4302544.1335459/94‑1 (MQ=60)

                                  TGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACT                                                              <  SRR4302544.3969216/94‑1 (MQ=60)

                                    TGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCG                                                            <  SRR4302544.2420995/94‑1 (MQ=60)

                                      GCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGAT                                                          <  SRR4302544.4512530/94‑1 (MQ=60)

                                       GGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATG                                                         <  SRR4302544.2642383/94‑1 (MQ=60)

                                       CGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGGATGGGAAATATGAGTTTAGCCCCCTCCTTACTCGATG                                                         >  SRR4302544.126613/1‑94 (MQ=60)

                                       CGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGGATGGAAAATATGAGTTTAGGCCCCTACTTACTCGCTG                                                         >  SRR4302544.3542422/1‑94 (MQ=60)

                                         CTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCA                                                       <  SRR4302544.3999828/94‑1 (MQ=60)

                                             CCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG                                                   <  SRR4302544.3801903/94‑1 (MQ=60)

                                             CCCCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGGGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG                                                   <  SRR4302544.2076194/94‑1 (MQ=60)

                                                 CCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGGATGGGAAATATGAGTTTAGCCCCCTACTTATTCGATGCAAAAGAACG                                               >  SRR4302544.1068247/1‑94 (MQ=60)

                                                 CCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGGGGCG‑GGGG‑GGGATGGGAAAAATGAGGTTAGCCCCCTACCTACCCGGTGCAAAAGgggc                                               >  SRR4302544.701840/1‑90 (MQ=39)

                                                  CAGGCCATTCAGTCATGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGC                                              <  SRR4302544.905242/94‑1 (MQ=60)

                                                  CAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGC                                              <  SRR4302544.1839719/94‑1 (MQ=60)

                                                   AGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGGATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGAAAAAGCACGC                                              >  SRR4302544.4023364/1‑94 (MQ=60)

                                                   AGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCT                                             <  SRR4302544.2237707/94‑1 (MQ=60)

                                                     GCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCT                                           <  SRR4302544.4506974/94‑1 (MQ=60)

                                                     GCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCT                                           <  SRR4302544.4506973/94‑1 (MQ=60)

                                                     GCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGGATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCT                                           >  SRR4302544.4184430/1‑94 (MQ=60)

                                                        ATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGGATGGAAAATATGAGGTTAGGCCCCTACTTACTCGATGCAAAAGCACGCTCTGGT                                        >  SRR4302544.4215002/1‑94 (MQ=60)

                                                            AGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC                                    <  SRR4302544.1906890/94‑1 (MQ=60)

                                                            AGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACCCTCTGGTTATC                                    >  SRR4302544.918954/1‑94 (MQ=60)

                                                             GGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCG                                   <  SRR4302544.2350776/94‑1 (MQ=60)

                                                               CAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTC                                 >  SRR4302544.4642238/1‑94 (MQ=60)

                                                                AGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑GGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCT                                >  SRR4302544.3293279/1‑94 (MQ=60)

                                                                  GTCAGGCGCAGGGCATTGTGGCG‑GGGG‑GGGATTGAAAATATGCGTTTAGCCCCCTACTTACTCGATGCAAAAGCACCCTCTGGTTATCGTCTTG                              >  SRR4302544.2278332/1‑94 (MQ=60)

                                                                    CAGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGA                            <  SRR4302544.4788964/94‑1 (MQ=60)

                                                                     AGGCGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAG                           <  SRR4302544.3955205/94‑1 (MQ=60)

                                                                        CGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACG                        >  SRR4302544.2894191/1‑94 (MQ=60)

                                                                        CGCAGAGCATTGTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACG                        <  SRR4302544.3298997/94‑1 (MQ=60)

                                                                         GCAGAGCATTGGGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGG                       <  SRR4302544.1308897/94‑1 (MQ=60)

                                                                          CAGAGCATTGTGGCG‑GGGG‑GGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGA                      >  SRR4302544.2141735/1‑94 (MQ=60)

                                                                             AGCATTGTGGCG‑GGGG‑GGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAG                   >  SRR4302544.3951402/1‑94 (MQ=60)

                                                                                 TTGTGGCGGGGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTT                <  SRR4302544.2614849/94‑1 (MQ=60)

                                                                                   GTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTAT             <  SRR4302544.3336675/94‑1 (MQ=60)

                                                                                   GTGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTAT             >  SRR4302544.3464307/1‑94 (MQ=60)

                                                                                    TGGCG‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATG            >  SRR4302544.223752/1‑94 (MQ=60)

                                                                                      GC‑‑GGGGTGGTATGGAAAATATGAGTTAAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGAC          <  SRR4302544.2601367/94‑1 (MQ=60)

                                                                                           GGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAA      <  SRR4302544.3295495/94‑1 (MQ=60)

                                                                                           GGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAA      >  SRR4302544.3670486/1‑94 (MQ=60)

                                                                                           GGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAA      >  SRR4302544.2754347/1‑94 (MQ=60)

                                                                                            GG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAAT     >  SRR4302544.3598229/1‑94 (MQ=60)

                                                                                            GG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAAT     <  SRR4302544.2458102/94‑1 (MQ=60)

                                                                                             G‑GGTATTGAAAATATGAGTGTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATC    <  SRR4302544.3971478/94‑1 (MQ=60)

                                                                                             G‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATC    <  SRR4302544.3377336/94‑1 (MQ=60)

                                                                                             G‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATC    <  SRR4302544.3971479/94‑1 (MQ=60)

                                                                                               GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCC   <  SRR4302544.4087419/94‑1 (MQ=60)

                                                                                                GTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  <  SRR4302544.501641/94‑1 (MQ=60)

                                                                                                GTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  <  SRR4302544.4514832/94‑1 (MQ=60)

CGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑GGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  >  NC_000913/2324376‑2324562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`